光遗传学和化学遗传学工具快速重新定位vimentin中间细丝。

IF 7.4 1区 生物学 Q1 CELL BIOLOGY
Journal of Cell Biology Pub Date : 2025-09-01 Epub Date: 2025-07-08 DOI:10.1083/jcb.202504004
Milena Pasolli, Joyce C M Meiring, James P Conboy, Gijsje H Koenderink, Anna Akhmanova
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引用次数: 0

摘要

中间丝(if)是细胞骨架的关键组成部分,对调节细胞力学、维持核完整性、细胞器定位和调节细胞信号传导至关重要。目前对中频功能的认识主要来自长期扰动的研究,如蛋白质消耗或突变。在这里,我们提出的工具,允许快速操作在整个细胞质或特定的亚细胞区域,通过诱导耦合到微管马达,药理学或使用光。vimentin的快速核周聚集对肌动蛋白或微管组织、细胞扩散或局灶黏附数没有重大的直接影响,但它降低了细胞的硬度。线粒体和内质网(ER)薄片由于波形蛋白聚集而重组,而溶酶体只是短暂移位并迅速恢复其正态分布。角蛋白在一些细胞系中随蛋白移动,但在另一些细胞系中保持完整。我们的工具有助于研究波形蛋白扰动的直接和局部影响,并确定波形蛋白与其他细胞结构的直接联系。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Optogenetic and chemical genetic tools for rapid repositioning of vimentin intermediate filaments.

Intermediate filaments (IFs) are a key component of the cytoskeleton, essential for regulating cell mechanics, maintaining nuclear integrity, organelle positioning, and modulating cell signaling. Current insights into IF function primarily come from studies using long-term perturbations, such as protein depletion or mutation. Here, we present tools that allow rapid manipulation of vimentin IFs in the whole cytoplasm or within specific subcellular regions by inducibly coupling them to microtubule motors, either pharmacologically or using light. Rapid perinuclear clustering of vimentin had no major immediate effects on the actin or microtubule organization, cell spreading, or focal adhesion number, but it reduced cell stiffness. Mitochondria and endoplasmic reticulum (ER) sheets were reorganized due to vimentin clustering, whereas lysosomes were only briefly displaced and rapidly regained their normal distribution. Keratin moved along with vimentin in some cell lines but remained intact in others. Our tools help to study the immediate and local effects of vimentin perturbation and identify direct links of vimentin to other cellular structures.

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来源期刊
Journal of Cell Biology
Journal of Cell Biology 生物-细胞生物学
CiteScore
12.60
自引率
2.60%
发文量
213
审稿时长
1 months
期刊介绍: The Journal of Cell Biology (JCB) is a comprehensive journal dedicated to publishing original discoveries across all realms of cell biology. We invite papers presenting novel cellular or molecular advancements in various domains of basic cell biology, along with applied cell biology research in diverse systems such as immunology, neurobiology, metabolism, virology, developmental biology, and plant biology. We enthusiastically welcome submissions showcasing significant findings of interest to cell biologists, irrespective of the experimental approach.
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