Intraflagellar transport selectivity occurs within the proximal portion of the trypanosome flagellum.

IF 6.4 1区生物学 Q1 CELL BIOLOGY

Journal of Cell Biology Pub Date : 2025-08-13 DOI:10.1083/jcb.202501088

Aline Araujo Alves,Jamin Jung,Gaël Moneron,Humbeline Vaucelle,Cécile Fort,Johanna Buisson,Cataldo Schietroma,Philippe Bastin

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引用次数: 0

Abstract

Intraflagellar transport (IFT) trains move bidirectionally along the doublet microtubules (DMTs) of the axoneme within the flagellum. In Trypanosoma brucei, IFT trains predominantly associate with four of the nine DMTs. Using high-resolution microscopy, we reveal how this selective association is put in place. IFT proteins form a ring surrounding the 9 DMTs above the transition fibers. Volume electron microscopy revealed densities along all DMTs in the proximal portion of the flagellum, exhibiting thinner, shorter profiles with branches absent in mature IFT trains. As the axoneme extends within the flagellar pocket, IFT trains are detected but are often positioned outside DMTs 3-4/7-8. After the axoneme exits the flagellar pocket, IFT trains localize exclusively to DMTs 3-4 and 7-8. Super-resolution and expansion microscopy demonstrated that IFT proteins follow the same distribution as the IFT-like densities. This suggests they represent IFT trains undergoing assembly and/or disassembly and reveals their unexpected ability to shift from one DMT to another.

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鞭毛内转运选择性发生在锥虫鞭毛的近端。

鞭毛内鞭毛内运输（IFT）列车沿着轴突的双态微管（dmt）双向移动。在布鲁氏锥虫中，IFT序列主要与9个dmt中的4个相关。使用高分辨率显微镜，我们揭示了这种选择性关联是如何实现的。IFT蛋白在过渡纤维上方的9个DMTs周围形成一个环。体积电镜显示，在鞭毛近端部分沿所有DMTs的密度，显示出更薄，更短的轮廓，分枝在成熟的IFT序列中缺失。当轴突在鞭毛囊内延伸时，可以检测到IFT序列，但通常位于dmt 3-4/7-8之外。轴素离开鞭毛囊后，IFT序列只定位于dmt 3-4和dmt 7-8。超分辨率和扩增显微镜显示，IFT蛋白遵循与IFT样密度相同的分布。这表明它们代表了正在组装和/或拆卸的IFT列车，并揭示了它们从一个DMT转移到另一个DMT的意外能力。

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来源期刊

Journal of Cell Biology 生物-细胞生物学

CiteScore

12.60

自引率

2.60%

发文量

213

审稿时长

1 months

期刊介绍： The Journal of Cell Biology (JCB) is a comprehensive journal dedicated to publishing original discoveries across all realms of cell biology. We invite papers presenting novel cellular or molecular advancements in various domains of basic cell biology, along with applied cell biology research in diverse systems such as immunology, neurobiology, metabolism, virology, developmental biology, and plant biology. We enthusiastically welcome submissions showcasing significant findings of interest to cell biologists, irrespective of the experimental approach.