Journal of bioscience and bioengineering最新文献

{"title":"Screening of Leuconostoc mesenteroides strains suitable for kimoto-style brewing of sake with high antioxidant capacity","authors":"Tomotsugu Noguchi,&nbsp;Keisuke Tobita","doi":"10.1016/j.jbiosc.2024.12.005","DOIUrl":"10.1016/j.jbiosc.2024.12.005","url":null,"abstract":"<div><div>Sake brewed using the <em>kimoto</em>-style exhibits high antioxidant capacity and is expected to inhibit the deterioration of sake quality due to oxidation. However, the antioxidant capacity of the added lactic acid bacteria has not been explored. We aimed to screen the lactic acid bacterium, <em>Leuconostoc mesenteroides</em>, with excellent brewing and antioxidant capacity, to develop sake with high antioxidant capacity. Three <em>Le. mesenteroides</em> strains (19-2, 19-5, and 19-23) were selected from eight screened strains based on their alcohol intolerance, growth performance in <em>koji</em> extract, aroma compound, and low biogenic amine production. Among these, <em>Le. mesenteroides</em> 19-23 exhibited a significantly higher hydrophilic-oxygen radical absorbance capacity (H-ORAC) in the culture medium than the control strain, <em>Le. mesenteroides</em> NBRC102481. In a medium-scale sake brewing test, sake prepared with <em>Le. mesenteroides</em> 19-23 had a significantly higher H-ORAC value than that prepared using the <em>sokujo</em>-style (without <em>Le. mesenteroides</em>). Additionally, metabolome analysis using capillary electrophoresis time-of-flight mass spectrometry identified ferulic acid, <em>p</em>-coumaric acid, 3-hydroxyanthranilic acid (3-HAA), and 6,8-thioctic acid in the main mash. High-performance liquid chromatography-based quantification revealed that antioxidants in the unrefined filtrates of the main mash prepared using the <em>kimoto</em>-style tended to be more abundant than in those prepared using the <em>sokujo</em>-style. Specifically, 3-HAA and ferulic acid were more concentrated in some unrefined filtrates of the <em>kimoto</em>-style than <em>sokujo</em>-style. In conclusion, the screened <em>Le. mesenteroides</em> strain demonstrated potential for brewing sake with high antioxidant capacity using the <em>kimoto</em>-style, offering a promising method for antioxidant-rich sake production.</div></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"139 3","pages":"Pages 213-218"},"PeriodicalIF":2.3,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142970919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization and application of Lachancea thermotolerans isolates for sake brewing","authors":"Miyu Nakatani ,&nbsp;Rina Ohtani ,&nbsp;Kiwamu Umezawa ,&nbsp;Taiyo Uchise ,&nbsp;Yoshifumi Matsuo ,&nbsp;Yasuhisa Fukuta ,&nbsp;Eri Obata ,&nbsp;Aruma Katabuchi ,&nbsp;Kento Kizaki ,&nbsp;Hana Kitazume ,&nbsp;Masataka Ohashi ,&nbsp;Katsuki Johzuka ,&nbsp;Atsushi Kurata ,&nbsp;Koichi Uegaki","doi":"10.1016/j.jbiosc.2024.10.004","DOIUrl":"10.1016/j.jbiosc.2024.10.004","url":null,"abstract":"<div><div>Non-conventional yeasts are increasingly being used in the production of fermented beverages owing to their ability to create unique and high-quality products. The yeast <em>Lachancea thermotolerans</em> is of great industrial significance, particularly in the production of <span>l</span>(+)-lactic acid, which is beneficial for acidifying wine, beer, and potentially sake. To explore its potential in sake brewing, three <em>L. thermotolerans</em> strains were isolated from natural environments and their physiological and fermentative characteristics were examined. The isolates surpassed the <em>L. thermotolerans</em> type strain (NBRC 1985) in lactic acid production under various culture conditions and exhibited comparable growth rates to that of <em>Saccharomyces cerevisiae</em> at 15–20 °C. Sake brewing tests using these isolates yielded approximately 3500 ppm of lactic acid, with a slightly lower production of aroma components compared to that produced by sake yeast, and an ethanol content of approximately 11–12 % was obtained. Reverse transcription-quantitative polymerase chain reaction revealed variable expression in putative lactate dehydrogenase genes depending on the culture conditions. Our findings suggest that <em>L. thermotolerans</em> strains can be used in sake brewing to produce unique sake.</div></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"139 1","pages":"Pages 30-35"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142568685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioconversion of eicosapentaenoic acid into 5S,15S- and 5R,15R-dihydroxyeicosapentaenoic acids by double-dioxygenating 15S- and 15R-lipoxygenases","authors":"Jin Lee ,&nbsp;Hyun-Ah Park ,&nbsp;Kyung-Chul Shin ,&nbsp;Deok-Kun Oh","doi":"10.1016/j.jbiosc.2024.09.002","DOIUrl":"10.1016/j.jbiosc.2024.09.002","url":null,"abstract":"<div><div>Resolvin E series (Rvs), such as RvE4 (5<em>S</em>,15<em>S</em>-dihydroxyeicosapentaenoic acid) and its stereoselective enantiomer (5<em>R</em>,15<em>R</em>-dihydroxyeicosapentaenoic acid), play an important role in promoting the resolution of inflammation and are derived from eicosapentaenoic acid (EPA) by M2 macrophage in human. However, they have been synthesized using expensive and inefficient chemical methods. Here, we performed efficient quantitative production of RvE4 and its enantiomer from EPA using <em>Escherichia coli</em> expressing double-dioxygenating 15<em>S</em>-lipoxygenase (15<em>S</em>-LOX) from <em>Archangium violaceum</em> and double-dioxygenating 15<em>R</em>-LOX from <em>Sorangium cellulosum</em>, respectively, with solvent, polymer, and adsorbent resin. The cell density, substrate concentration, solvent types and concentrations, polymer types and concentrations, and resin concentration were optimized for the enhanced bioconversion of EPA into RvE4 and its enantiomer. Under the optimized conditions, <em>A. violaceum</em> 15<em>S</em>-LOX and <em>S. cellulosum</em> 15<em>R</em>-LOX expressed in <em>E. coli</em> converted 6.0 mM EPA into 4.3 mM (1.44 g/L) RvE4 and 5.8 mM (1.94 g/L) RvE4 enantiomer in 60 min, with productivities of 4.3 and 5.8 mM/h and molar conversions of 72% and 97%, respectively. To date, these are the highest concentrations, productivities, and conversions of RvE4 and its enantiomer. The concentrations of RvE4 and its enantiomer obtained from the conversion of EPA with solvent, polymer, and resin were 2.5- and 3.2-fold higher than those without the additives, respectively.</div></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"139 1","pages":"Pages 1-6"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142466296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aromatic residues in the oligonucleotide binding domain are essential to the function of the single-stranded DNA binding protein of Helicobacter pylori","authors":"Mon-Juan Lee ,&nbsp;Li-Kun Huang ,&nbsp;Wen-Hsin Huang ,&nbsp;Po-Yu Chan ,&nbsp;Zi-Sin Yang ,&nbsp;Ching-Ming Chien ,&nbsp;Ching-Chang Chieng ,&nbsp;Haimei Huang","doi":"10.1016/j.jbiosc.2024.09.003","DOIUrl":"10.1016/j.jbiosc.2024.09.003","url":null,"abstract":"<div><div>Single-stranded DNA-binding protein (SSB) is essential to DNA replication, DNA repair, and homologous genetic recombination. Our previous study on the crystal structure of a C-terminally truncated SSB from <em>Helicobacter pylori</em>, HpSSBc, in complex with single-stranded DNA (ssDNA) suggests that several aromatic residues, including Phe37, Phe50, Phe56, and Trp84, were involved in ssDNA binding. To investigate the importance of these aromatic residues, the binding activity of four site-directed HpSSB mutants, including F37A HpSSB, F50A HpSSB, F56A HpSSB, and W84A HpSSB, was compared to that of wild-type HpSSB and HpSSBc by means of electrophoresis mobility shift assay (EMSA), tryptophan quenching fluorescence titration, and surface plasmon resonance (SPR). Molecular docking and molecular dynamic (MD) simulation of a F37A and a quadruple mutation model of HpSSBc support that the ssDNA-HpSSBc complex was destabilized when either one or four of the aromatic residues were mutated. The findings of this study suggest that mutation of the phenylalanine and tryptophan residues within the oligonucleotide-binding domain significantly diminished the ssDNA binding capability of HpSSB, highlighting the crucial role these aromatic residues play in the binding of ssDNA by HpSSB.</div></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"139 1","pages":"Pages 7-13"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142568681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Positive impact of pyrocarbon and mechanical loading on cartilage-like tissue synthesis in a scaffold-free process","authors":"Imbert De Gaudemaris ,&nbsp;Amira Hannoun ,&nbsp;Rémy Gauthier ,&nbsp;Nina Attik ,&nbsp;Leyre Brizuela ,&nbsp;Saida Mebarek ,&nbsp;Michel Hassler ,&nbsp;Carole Bougault ,&nbsp;Ana-Maria Trunfio-Sfarghiu","doi":"10.1016/j.jbiosc.2024.09.005","DOIUrl":"10.1016/j.jbiosc.2024.09.005","url":null,"abstract":"<div><div>Aiming to build a tissue analogue engineered cartilage from differentiated chondrocytes, we investigated the potential of a pyrocarbon (PyC)-based and scaffold-free process, under mechanical stimulation. PyC biomaterial has shown promise in arthroplasty and implant strategies, and mechanical stimulation is recognized as an improvement in regeneration strategies. The objective was to maintain the cell phenotype to produce constructs with cartilage-like matrix composition and mechanical properties. Primary murine chondrocytes were deposited in drop form between two biomaterial surfaces expanded to 500 μm and a uniaxial cyclic compression was applied thanks to a handmade tribo-bioreactor (0.5 Hz, 100 μm of amplitude, 17 days). Histology and immunohistochemistry analysis showed that PyC biomaterial promoted expression of cartilage-like matrix components (glycosaminoglycans, type II collagen, aggrecan). Importantly, constructs obtained in dynamic conditions were denser and showed a cohesive and compact shape. The most promising condition was the combined use of PyC and dynamic stimulation, resulting in constructs of low elasticity and high viscosity, thus with an increased damping factor. We verified that no calcium deposits were detectable and that type X collagen was not expressed, suggesting that the cells had not undergone hypertrophic maturation. While most studies focus on the comparison of different biomaterials or on the effect of different mechanical stimuli separately, we demonstrated the value of combining the two approaches to get as close as possible to the biological and mechanical qualities of natural hyaline articular cartilage.</div></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"139 1","pages":"Pages 53-59"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142466308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular mechanisms of complex-type N-glycan breakdown and metabolism by the human intestinal bacterium Barnesiella intestinihominis","authors":"Kanako Doi,&nbsp;Kazuki Mori,&nbsp;Misaki Komatsu,&nbsp;Akari Shinoda,&nbsp;Kosuke Tashiro,&nbsp;Yujiro Higuchi,&nbsp;Jiro Nakayama,&nbsp;Kaoru Takegawa","doi":"10.1016/j.jbiosc.2024.10.006","DOIUrl":"10.1016/j.jbiosc.2024.10.006","url":null,"abstract":"<div><div>Intestinal bacteria play a crucial role in human health, for example, by maintaining immune and metabolic homeostasis and protecting against pathogens. Survival in the human intestine depends on the bacterium's ability to utilize complex carbohydrates. Some species are known to use host-derived glycans; for example, <em>Bifidobacteria</em> can utilize <em>O</em>-glycan of mucin. However, there are few studies on intestinal bacteria utilizing host-derived <em>N</em>-glycan. Here, we identified the mechanism underlying the breakdown and utilization of complex-type <em>N</em>-glycan by the human intestinal bacterium <em>Barnesiella intestinihominis</em>. A growth assay showed that <em>B. intestinihominis</em> can utilize complex-type <em>N</em>-glycan as a carbon source, while RNA-seq analysis identified enzymes and transporters involved in the mechanism of <em>N</em>-glycan breakdown. In particular, the expression of three genes encoding glycoside hydrolase 85 endo-β<em>-N</em>-acetylglucosaminidase (<em>endo-BIN1</em>, <em>endo-BIN2</em>, and <em>endo-BIN3</em>) rose markedly in bacterial cells cultured in complex-type N-glycoprotein medium. We also found that the <em>susC</em> and <em>susD</em> genes, encoding the SusC/SusD membrane complex, form a gene cluster with <em>endo-BIN</em> genes, suggesting that SusC/SusD is involved in transportation of the glycan into the cell. Other genes encoding exo-type glycoside hydrolase enzymes showed elevated expression in cells grown in complex-type N-glycoprotein medium, suggesting that these enzymes function in further degradation of glycan for metabolism by the bacterium. Collectively, these findings suggest the survival strategy of an intestinal bacterium that has a unique metabolic pathway to use host-derived complex-type <em>N</em>-glycan as a nutrient.</div></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"139 1","pages":"Pages 14-22"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142604889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of induced pluripotent stem cell differentiation into neural progenitor cell using Raman spectra derived from extracellular vesicles in culture supernatants","authors":"Kakuro Hirai ,&nbsp;Hikaru Saito ,&nbsp;Midori Kato ,&nbsp;Masaharu Kiyama ,&nbsp;Hiroko Hanzawa ,&nbsp;Atsushi Nakane ,&nbsp;Sayaka Sekiya ,&nbsp;Kenji Yoshida ,&nbsp;Akiyoshi Kishino ,&nbsp;Atsushi Ikeda ,&nbsp;Toru Kimura ,&nbsp;Jun Takahashi ,&nbsp;Shizu Takeda","doi":"10.1016/j.jbiosc.2024.09.004","DOIUrl":"10.1016/j.jbiosc.2024.09.004","url":null,"abstract":"<div><div>Non-invasive cell culture monitoring technology is crucial to improve the manufacturing efficiency of cell products. We have found that extracellular vesicles (EVs) are secreted into the culture supernatants in the differentiation process from human induced pluripotent stem cells (iPSCs) to dopaminergic progenitor cells, and that the composition of EVs changes in accordance with the differentiation processes. In this study, we hypothesized that it is possible to evaluate the cultured cellular states by detecting compositional changes of EVs secreted from cultured cells with label-free Raman spectroscopy in a non-invasive manner. Therefore, Raman signal analysis derived from EV fractions isolated from culture supernatants throughout the differentiation process was conducted. iPSCs cultures were simultaneously implemented under a standard condition (control) and an artificial deviation condition inducing reductions in pluripotency by depleting FGF2 in culture medium (-FGF2), which is indispensable for maintaining the pluripotency. Subsequently, the differentiation step was conducted for each iPSCs culture under the same condition. As a result, it was found that under -FGF2, the expression level of the pluripotency marker <em>NANOG</em> decreased compared to that of the control and correlated with the identification results based on Raman signals with a correlation coefficient of 0.77. Lipid-derived Raman signals were extracted as identification factors, suggesting that changes in the lipid component of EV occur depending on the cellular states. From the above, we have found that the change in composition of EVs in the culture supernatant by detecting Raman signals would be a monitoring index of the cellular state of differentiation and pluripotency.</div></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"139 1","pages":"Pages 44-52"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142466297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mass spectrometry imaging of gamma-aminobutyric acid and glutamic acid decarboxylase reactions at various stages of banana ripening","authors":"Shiho Ishimoto ,&nbsp;Eiichiro Fukusaki ,&nbsp;Shuichi Shimma","doi":"10.1016/j.jbiosc.2024.10.001","DOIUrl":"10.1016/j.jbiosc.2024.10.001","url":null,"abstract":"<div><div>Banana is the fourth most consumed crop worldwide, and its high economic value and health benefits have made it very popular. Bananas are climacteric fruits that ripen after harvesting. It has been reported that the endogenous substances in bananas change significantly during the ripening process. This study focused on levels of gamma-aminobutyric acid (GABA) and glutamic acid decarboxylase (GAD), an enzyme that catalyzes the synthesis of GABA, which reportedly fluctuates during the ripening stage. Previous studies have shown that GAD expression is associated with banana ripening; however, changes in its distribution during ripening have not been verified. This study aimed to clarify the relationship between GABA and GAD during ripening of ethylene-treated bananas. Visualization of the localization of endogenous GABA and GAD was performed using mass spectrometry imaging. To visualize GAD reaction, a glutamate-d₃ (labeled substrate) was supplied to the sample, and a GABA-d₃ (labeled product) was regarded as the localization of the enzymatic reaction. Liquid chromatography-mass spectrometry was also used to confirm the amount of GABA and activity of the GAD. This will allow us to clarify the direct relationship between GABA and GAD and to understand the role of the GAD reaction in phytohormones.</div></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"139 1","pages":"Pages 79-84"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142557905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plasma-activated medium suppresses proliferation and migration of human lung cancer cells by regulating PI3K/AKT-Wnt signaling pathway","authors":"Zhidan Sun ,&nbsp;Chenglong Ding ,&nbsp;Yuhan Wang ,&nbsp;Han Zhou ,&nbsp;Wencheng Song","doi":"10.1016/j.jbiosc.2024.10.002","DOIUrl":"10.1016/j.jbiosc.2024.10.002","url":null,"abstract":"<div><div>The main causes of high mortality in lung cancer patients are the malignant growth and migration of cancer cells. This study aims to investigate the underlying mechanisms of low-temperature plasma-activated medium (PAM) treating human lung cancer (HLC). Changes in the levels of reactive oxygen and nitrogen species both inside and outside the cells were evaluated. Our results showed that prolonged PAM exposure decreased cell viability, raised intracellular reactive oxygen species levels, and hindered cell migration while reducing mitochondrial membrane potential. Protein analysis revealed PAM increased GSK-3β and p-β-catenin expression but decreased PI3K, AKT, p-AKT, p-GSK-3β, Wnt, and β-catenin levels, thereby inhibiting the epithelial–mesenchymal transition. These findings suggest PAM suppresses HLC cells proliferation and migration by blocking the PI3K/AKT-Wnt pathway. The study will provide a valuable theoretical basis for future low-temperature plasma treatment, thereby improving the survival rates and prognosis of lung cancer.</div></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"139 1","pages":"Pages 60-69"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142621139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of a low acetate-producing strain of Tetragenococcus halophilus to soy sauce fermentation","authors":"Keita Higuchi ,&nbsp;Yuya Nukagawa ,&nbsp;Takura Wakinaka ,&nbsp;Jun Watanabe ,&nbsp;Yoshinobu Mogi","doi":"10.1016/j.jbiosc.2024.09.007","DOIUrl":"10.1016/j.jbiosc.2024.09.007","url":null,"abstract":"<div><div>In soy sauce brewing, the halophilic lactic acid bacterium, <em>Tetragenococcus halophilus</em> is used as a fermentation starter and contributes to the taste and aroma of soy sauce, mainly by producing lactate. By lowering the pH of the soy sauce mash, lactate serves as a suitable growth environment for the halotolerant yeast <em>Zygosaccharomyces rouxii</em>. Acetate, which is produced by <em>T. halophilus</em> via the citrate metabolic pathway, is a critical growth inhibitory factor for <em>Z. rouxii</em>. Therefore, a <em>T. halophilus</em> strain that lacks acetate production could be an ideal fermentation starter to enhance ethanol production. In this study, we obtained a derivative of <em>T. halophilus</em> containing an insertion sequence in <em>citC</em>, which is an essential gene for citrate metabolism, and validated its performance as a soy sauce fermentation starter. The derivative neither metabolized citrate nor produced excessive acetate in soy sauce mash, resulting in vigorous alcohol fermentation by <em>Z. rouxii</em>. This study provides insights into the application of a low acetate-producing strain of <em>T. halophilus</em> as a starter to produce soy sauce with high alcohol content and low sour aroma.</div></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"139 1","pages":"Pages 23-29"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142466295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}