Journal of bioscience and bioengineering最新文献_第10页

Recent advances in plant-based bioproduction 植物生物生产的最新进展。

IF 2.8 4区生物学

Journal of bioscience and bioengineering Pub Date : 2024-04-13 DOI: 10.1016/j.jbiosc.2024.01.007

Kazuhito Fujiyama , Toshiya Muranaka , Atsushi Okazawa , Hikaru Seki , Goro Taguchi , Shuhei Yasumoto

引用次数: 0

Characteristics of kopyor coconut (Cocos nucifera L.) using sensory analysis and metabolomics-based approach 利用感官分析和代谢组学方法分析椰子（Cocos nucifera L.）的特征。

IF 2.8 4区生物学

Journal of bioscience and bioengineering Pub Date : 2024-04-12 DOI: 10.1016/j.jbiosc.2024.02.008

Mercy Bientri Yunindanova , Sastia Prama Putri , Hengky Novarianto , Eiichiro Fukusaki

{"title":"Characteristics of kopyor coconut (Cocos nucifera L.) using sensory analysis and metabolomics-based approach","authors":"Mercy Bientri Yunindanova , Sastia Prama Putri , Hengky Novarianto , Eiichiro Fukusaki","doi":"10.1016/j.jbiosc.2024.02.008","DOIUrl":"10.1016/j.jbiosc.2024.02.008","url":null,"abstract":"<div>Kopyor is a coconut with unique characteristics from Indonesia, one of the largest coconut producers in the world. Kopyor is an edible mature coconut with soft endosperm. Although this fruit is one of the most popular coconuts in the world, there are limited studies on its properties, including its sensory attributes and metabolite profiles. This study investigates the characteristics of kopyor using sensory evaluation, a widely targeted metabolomics approach, and multivariate analysis. The liquid (water) and solid (flesh) endosperms were collected as the samples. The results showed that kopyor has characteristics that distinguish it from normal mature and young coconuts. Kopyor water has a milky, creamy, nutty, bitter, and astringent taste with an oily aftertaste and mouthfeel. Kopyor flesh is soft and moist and gives a sandy mouth feel. This study analyzed the sensory attributes of the kopyor endosperm for the first time and compared it with those of normal mature and young coconuts. A gas chromatography mass spectrometry analysis showed that kopyor contained wider variety of metabolites than normal coconuts of the same age. Based on the differential analysis and orthogonal projections to latent structures-regression, kopyor water was characterized by the accumulation of flavor-related metabolites, such as amino acids and organic acids, which contributed to its sensory complexity. This study solidified the effects of maturation and endosperm type on metabolite accumulation in kopyor endosperm. This pioneering information will lead to the future use of kopyor and other unique coconuts worldwide for food, contributing to the sustainability of the coconut industry.</div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"138 1","pages":"Pages 44-53"},"PeriodicalIF":2.8,"publicationDate":"2024-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140759897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bone morphogenetic protein signaling inhibitor improves differentiation and function of 3D muscle construct fabricated using C2C12 骨形态发生蛋白信号抑制剂改善了使用 C2C12 制作的三维肌肉结构的分化和功能。

IF 2.8 4区生物学

Journal of bioscience and bioengineering Pub Date : 2024-04-10 DOI: 10.1016/j.jbiosc.2024.03.005

Ran Ding , Yuan Xi , Akira Ito , Kazunori Shimizu , Eiji Nagamori , Hideaki Fujita , Takuo Kawamoto , Masanobu Horie

{"title":"Bone morphogenetic protein signaling inhibitor improves differentiation and function of 3D muscle construct fabricated using C2C12","authors":"Ran Ding , Yuan Xi , Akira Ito , Kazunori Shimizu , Eiji Nagamori , Hideaki Fujita , Takuo Kawamoto , Masanobu Horie","doi":"10.1016/j.jbiosc.2024.03.005","DOIUrl":"10.1016/j.jbiosc.2024.03.005","url":null,"abstract":"<div>Functional tissue-engineered artificial skeletal muscle tissue has great potential for pharmacological and academic applications. This study demonstrates an in vitro tissue engineering system to construct functional artificial skeletal muscle tissues using self-organization and signal inhibitors. To induce efficient self-organization, we optimized the substrate stiffness and extracellular matrix (ECM) coatings. We modified the tissue morphology to be ring-shaped under optimized self-organization conditions. A bone morphogenetic protein (BMP) inhibitor was added to improve overall myogenic differentiation. This supplementation enhanced the myogenic differentiation ratio and myotube hypertrophy in two-dimensional cell cultures. Finally, we found that myotube hypertrophy was enhanced by a combination of self-organization with ring-shaped tissue and a BMP inhibitor. BMP inhibitor treatment significantly improved myogenic marker expression and contractile force generation in the self-organized tissue. These observations indicated that this procedure may provide a novel and functional artificial skeletal muscle for pharmacological studies.</div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"137 6","pages":"Pages 480-486"},"PeriodicalIF":2.8,"publicationDate":"2024-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140779158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of microbial dynamics in the soybean root-associated environments from community to single-cell levels 从群落到单细胞水平分析大豆根相关环境中的微生物动态。

IF 2.8 4区生物学

Journal of bioscience and bioengineering Pub Date : 2024-04-04 DOI: 10.1016/j.jbiosc.2024.02.007

Masako Kifushi , Yohei Nishikawa , Masahito Hosokawa , Keigo Ide , Masato Kogawa , Toyoaki Anai , Haruko Takeyama

{"title":"Analysis of microbial dynamics in the soybean root-associated environments from community to single-cell levels","authors":"Masako Kifushi , Yohei Nishikawa , Masahito Hosokawa , Keigo Ide , Masato Kogawa , Toyoaki Anai , Haruko Takeyama","doi":"10.1016/j.jbiosc.2024.02.007","DOIUrl":"10.1016/j.jbiosc.2024.02.007","url":null,"abstract":"<div>Plant root-associated environments such as the rhizosphere, rhizoplane, and endosphere, are notably different from non-root-associated soil environments. However, the microbial dynamics in these spatially divided compartments remain unexplored. In this study, we propose a combinational analysis of single-cell genomics with 16S rRNA gene sequencing. This method enabled us to understand the entire soil microbiome and individual root-associated microorganisms. We applied this method to soybean microbiomes and revealed that their composition was different between the rhizoplane and rhizosphere in the early growth stages, but became more similar as growth progressed. In addition, a total of 610 medium- to high-quality single-amplified genomes (SAGs) were acquired, including plant growth-promoting rhizobacteria (PGPR) candidates while genomes with high GC content tended to be missed by SAGs. The whole-genome analyses of the SAGs suggested that rhizoplane-enriched Flavobacterium solubilizes organophosphate actively and Bacillus colonizes roots more efficiently. Single-cell genomics, together with 16S rRNA gene sequencing, enabled us to connect microbial taxonomy and function, and assess microorganisms at a strain resolution even in the complex soil microbiome.</div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"137 6","pages":"Pages 429-436"},"PeriodicalIF":2.8,"publicationDate":"2024-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140790684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and characterization of novel N-acylhomoserine lactonase from nonpathogenic Allorhizobium vitis, a candidate for biocontrol agent 非致病性葡萄全缘菌中新型 N-酰基高丝氨酸内酯酶的鉴定和特征描述，它是一种候选生物控制剂。

IF 2.8 4区生物学

Journal of bioscience and bioengineering Pub Date : 2024-04-04 DOI: 10.1016/j.jbiosc.2024.03.001

Tomohiro Morohoshi , Koki Hirose , Nobutaka Someya

{"title":"Identification and characterization of novel N-acylhomoserine lactonase from nonpathogenic Allorhizobium vitis, a candidate for biocontrol agent","authors":"Tomohiro Morohoshi , Koki Hirose , Nobutaka Someya","doi":"10.1016/j.jbiosc.2024.03.001","DOIUrl":"10.1016/j.jbiosc.2024.03.001","url":null,"abstract":"<div>Some strains of nonpathogenic Allorhizobium vitis can control crown gall disease in grapevines caused by pathogenic A. vitis and are considered candidates for biocontrol agents. Many plant pathogenic bacteria regulate the expression of their virulence genes via quorum sensing using N-acylhomoserine lactone (AHL) as a signaling compound. The eight nonpathogenic A. vitis strains used in this study showed AHL-degrading activity. The complete genome sequence of A. vitis MAFF 212306 contained three AHL lactonase gene homologs. When these genes were cloned and transformed into Escherichia coli DH5α, E. coli harboring the aiiV gene (RvVAR031_27660) showed AHL-degrading activity. The aiiV coding region was successfully amplified by polymerase chain reaction from the genomes of all eight strains of nonpathogenic A. vitis. Purified His-tagged AiiV exhibited AHL lactonase activity by hydrolyzing the lactone ring of AHL. AiiV had an optimal temperature of approximately 30 °C; however, its thermostability decreased above 40 °C. When the AiiV-expressing plasmid was transformed into Pectobacterium carotovorum subsp. carotovorum NBRC 3830, AHL production by NBRC 3830 decreased below the detection limit, and its maceration activity, which was controlled by quorum sensing, almost disappeared. These results suggest the potential use of AHL-degrading nonpathogenic A. vitis for the inhibition of crown gall disease in grapevines and other plant diseases controlled by quorum sensing.</div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"137 6","pages":"Pages 437-444"},"PeriodicalIF":2.8,"publicationDate":"2024-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140860259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Interpenetrating gelatin/alginate mixed hydrogel: The simplest method to prepare an autoclavable scaffold 互穿明胶/精氨酸混合水凝胶：制备可高压灭菌支架的最简单方法。

IF 2.8 4区生物学

Journal of bioscience and bioengineering Pub Date : 2024-04-04 DOI: 10.1016/j.jbiosc.2024.01.015

Hideki Mori, Yaya Taketsuna, Kae Shimogama, Koki Nishi, Masayuki Hara

{"title":"Interpenetrating gelatin/alginate mixed hydrogel: The simplest method to prepare an autoclavable scaffold","authors":"Hideki Mori, Yaya Taketsuna, Kae Shimogama, Koki Nishi, Masayuki Hara","doi":"10.1016/j.jbiosc.2024.01.015","DOIUrl":"10.1016/j.jbiosc.2024.01.015","url":null,"abstract":"<div>The choice of sterilization method for hydrogels used for cell culture influences the ease of preparing the gel. We prepared interpenetrating gelatin/calcium alginate hydrogels containing 1% (w/v) alginate and 1–16% (w/v) gelatin by molding with the mixture of gelatin/sodium alginate solution, followed by the addition of calcium ions by incubation in calcium chloride solution. It is the simplest method to prepare autoclavable gelatin/sodium hydrogel. We measured various properties of the hydrogels including volume, Young's modulus in the compression test, storage modulus, and loss modulus in the dynamic viscoelasticity measurement. The gelatin/alginate hydrogel can be easily fabricated into any shape by this method. After autoclave treatment, the hydrogel was shrunk to smaller than the original shape in similar figures. The shape of the gelatin/alginate hydrogel can be designed into any shape with the reduction ratio of the volume. Human osteosarcoma (HOS) cells adhered to the gelatin/alginate hydrogel and then proliferated. Gelatin/calcium alginate hydrogels with a high concentration are considered to be autoclavable culture substrates because of their low deformation and gelatin elution rate after autoclaving and the high amount of cells attached to the hydrogels.</div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"137 6","pages":"Pages 463-470"},"PeriodicalIF":2.8,"publicationDate":"2024-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140764538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Non-pathogenic Heyndrickxia coagulans (Bacillus coagulans) 29-2E inhibits the virulence of pathogenic Salmonella Typhimurium by quorum-sensing regulation 非致病性凝结芽孢杆菌（Heyndrickxia coagulans）29-2E通过定量感应调控抑制致病性鼠伤寒沙门氏菌的毒力。

IF 2.8 4区生物学

Journal of bioscience and bioengineering Pub Date : 2024-03-28 DOI: 10.1016/j.jbiosc.2024.03.002

Masafumi Noda , Shino Noguchi , Narandalai Danshiitsoodol , Toshinori Hara , Masanori Sugiyama

{"title":"Non-pathogenic Heyndrickxia coagulans (Bacillus coagulans) 29-2E inhibits the virulence of pathogenic Salmonella Typhimurium by quorum-sensing regulation","authors":"Masafumi Noda , Shino Noguchi , Narandalai Danshiitsoodol , Toshinori Hara , Masanori Sugiyama","doi":"10.1016/j.jbiosc.2024.03.002","DOIUrl":"10.1016/j.jbiosc.2024.03.002","url":null,"abstract":"<div>Bacteria produce and release small signal molecules, autoinducers, as an indicator of their cell density. The system, called a quorum-sensing (QS) system, is used to control not only virulence factors but also antibiotic production, sporulation, competence, and biofilm formation in bacteria. Different from antibiotics, QS inhibitors are expected to specifically repress the virulence factors in pathogenic bacteria without inhibiting growth or bactericidal effects. Therefore, since QS inhibitors have little risk of antibiotic-resistant bacteria emergence, they have been proposed as promising anti-bacterial agents. In the present study, we aimed to find new QS inhibitors that prohibit the signaling cascade of autoinducer 3 (AI-3) recognized by a QseCB two-component system that regulates some virulence factors of pathogens, such as enterohemorrhagic Escherichia coli (EHEC) and Salmonella enterica subsp. enterica serovar Typhimurium. We have established the method for QS-inhibitor screening using a newly constructed plasmid pLES-AQSA. E. coli DH5α transformed with the pLES-AQSA can produce β-galactosidase that converts 5-bromo-4-chloro-3-indolyl β-d-galactopyranoside (X-gal) into blue pigment (5-bromo-4-chloro-indoxyl) under the control of the QseCB system. By screening, Heyndrickxia coagulans (formerly Bacillus coagulans) 29-2E was found to produce an exopolysaccharide (EPS)-like water-soluble polymer that prohibits QseCB-mediated β-galactosidase production without antibacterial activities. Further, the simultaneous injection of the 29-2E strain significantly improves the survival rate of Salmonella Typhimurium-infected silkworm larvae (from 0% to 83.3%), suggesting that the substance may be a promising inhibitor against the virulence of pathogens without risk of the emergence of antibiotic-resistant bacteria.</div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"137 6","pages":"Pages 445-452"},"PeriodicalIF":2.8,"publicationDate":"2024-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140326605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Physiological evaluation of yeast strains under anaerobic conditions using glucose, fructose, or sucrose as the carbon source 使用葡萄糖、果糖或蔗糖作为碳源，对厌氧条件下的酵母菌株进行生理学评估。

IF 2.8 4区生物学

Journal of bioscience and bioengineering Pub Date : 2024-03-16 DOI: 10.1016/j.jbiosc.2024.02.003

Cinthia Aparecida de Andrade Silva , Marta Ligia Oka , Pedro Garcia Pereira da Silva , Janaina Mayumi Honma , Rodrigo Simões Ribeiro Leite , Gustavo Graciano Fonseca

{"title":"Physiological evaluation of yeast strains under anaerobic conditions using glucose, fructose, or sucrose as the carbon source","authors":"Cinthia Aparecida de Andrade Silva , Marta Ligia Oka , Pedro Garcia Pereira da Silva , Janaina Mayumi Honma , Rodrigo Simões Ribeiro Leite , Gustavo Graciano Fonseca","doi":"10.1016/j.jbiosc.2024.02.003","DOIUrl":"10.1016/j.jbiosc.2024.02.003","url":null,"abstract":"<div>The aim of this study was to evaluate the physiology of 13 yeast strains by assessing their kinetic parameters under anaerobic conditions. They included Saccharomyces cerevisiae CAT-1 and 12 isolated yeasts from different regions in Brazil. The study aimed to enhance understanding of the metabolism of these strains for more effective applications. Measurements included quantification of sugars, ethanol, glycerol, and organic acids. Various kinetic parameters were analyzed, such as specific substrate utilization rate (qS), maximum specific growth rate (μmax), doubling time, biomass yield, product yield, maximum cell concentration, ethanol productivity (PEth), biomass productivity, and CO2 concentration. S. cerevisiae CAT-1 exhibited the highest values in glucose for μmax (0.35 h−1), qS (3.06 h−1), and PEth (0.69 gEth L−1 h−1). Candida parapsilosis Recol 37 did not fully consume the substrate. In fructose, S. cerevisiae CAT-1 stood out with higher values for μmax (0.25 h−1), qS (2.24 h−1), and PEth (0.60 gEth L−1 h−1). Meyerozyma guilliermondii Recol 09 and C. parapsilosis Recol 37 had prolonged fermentation times and residual substrate. In sucrose, only S. cerevisiae CAT-1, S. cerevisiae BB9, and Pichia kudriavzevii Recol 39 consumed all the substrate, displaying higher PEth (0.72, 0.51, and 0.44 gEth L−1 h−1, respectively) compared to other carbon sources.</div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"137 6","pages":"Pages 420-428"},"PeriodicalIF":2.8,"publicationDate":"2024-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140140189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gene identification and enzymatic characterization of the initial enzyme in pyrimidine oxidative metabolism, uracil-thymine dehydrogenase 嘧啶氧化代谢初始酶--尿嘧啶-胸腺嘧啶脱氢酶的基因鉴定和酶学特征。

IF 2.8 4区生物学

Journal of bioscience and bioengineering Pub Date : 2024-03-13 DOI: 10.1016/j.jbiosc.2024.02.004

Chee-Leong Soong , Kengo Deguchi , Michiki Takeuchi , Syoko Kozono , Nobuyuki Horinouchi , Dayong Si , Makoto Hibi , Sakayu Shimizu , Jun Ogawa

{"title":"Gene identification and enzymatic characterization of the initial enzyme in pyrimidine oxidative metabolism, uracil-thymine dehydrogenase","authors":"Chee-Leong Soong , Kengo Deguchi , Michiki Takeuchi , Syoko Kozono , Nobuyuki Horinouchi , Dayong Si , Makoto Hibi , Sakayu Shimizu , Jun Ogawa","doi":"10.1016/j.jbiosc.2024.02.004","DOIUrl":"10.1016/j.jbiosc.2024.02.004","url":null,"abstract":"<div>Uracil-thymine dehydrogenase (UTDH), which catalyzes the irreversible oxidation of uracil to barbituric acid in oxidative pyrimidine metabolism, was purified from Rhodococcus erythropolis JCM 3132. The finding of unusual stabilizing conditions (pH 11, in the presence of NADP+ or NADPH) enabled the enzyme purification. The purified enzyme was a heteromer consisting of three different subunits. The enzyme catalyzed oxidation of uracil to barbituric acid with artificial electron acceptors such as methylene blue, phenazine methosulfate, benzoquinone, and α-naphthoquinone; however, NAD+, NADP+, flavin adenine dinucleotide, and flavin mononucleotide did not serve as electron acceptors. The enzyme acted not only on uracil and thymine but also on 5-halogen-substituted uracil and hydroxypyrimidine (pyrimidone), while dihydropyrimidine, which is an intermediate in reductive pyrimidine metabolism, and purine did not serve as substrates. The activity of UTDH was enhanced by cerium ions, and this activation was observed with all combinations of substrates and electron acceptors.</div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"137 6","pages":"Pages 413-419"},"PeriodicalIF":2.8,"publicationDate":"2024-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140131554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishment of a novel cell line, CHO-MK, derived from Chinese hamster ovary tissues for biologics manufacturing 从中国仓鼠卵巢组织中提取新型细胞系 CHO-MK，用于生物制剂生产。

IF 2.8 4区生物学

Journal of bioscience and bioengineering Pub Date : 2024-03-12 DOI: 10.1016/j.jbiosc.2024.02.005

Kenji Masuda , Michi Kubota , Yuto Nakazawa , Chigusa Iwama , Kazuhiko Watanabe , Naoto Ishikawa , Yumiko Tanabe , Satoru Kono , Hiroki Tanemura , Shinichi Takahashi , Tomohiro Makino , Takeshi Okumura , Takayuki Horiuchi , Koichi Nonaka , Sei Murakami , Masamichi Kamihira , Takeshi Omasa

{"title":"Establishment of a novel cell line, CHO-MK, derived from Chinese hamster ovary tissues for biologics manufacturing","authors":"Kenji Masuda , Michi Kubota , Yuto Nakazawa , Chigusa Iwama , Kazuhiko Watanabe , Naoto Ishikawa , Yumiko Tanabe , Satoru Kono , Hiroki Tanemura , Shinichi Takahashi , Tomohiro Makino , Takeshi Okumura , Takayuki Horiuchi , Koichi Nonaka , Sei Murakami , Masamichi Kamihira , Takeshi Omasa","doi":"10.1016/j.jbiosc.2024.02.005","DOIUrl":"10.1016/j.jbiosc.2024.02.005","url":null,"abstract":"<div>Chinese hamster ovary (CHO) cells are widely used as a host for producing recombinant therapeutic proteins due to advantages such as human-like post-translational modification, correct protein folding, higher productivity, and a proven track record in biopharmaceutical development. Much effort has been made to improve the process of recombinant protein production, in terms of its yield and productivity, using conventional CHO cell lines. However, to the best of our knowledge, no attempts have been made to acquire new CHO cell lines from Chinese hamster ovary. In this study, we established and characterized a novel CHO cell line, named CHO-MK, derived from freshly isolated Chinese hamster ovary tissues. Some immortalized cell lines were established via sub-culture derived from primary culture, one of which was selected for further development toward a unique expression system design. After adapting serum-free and suspension culture conditions, the resulting cell line exhibited a considerably shorter doubling time (approximately 10 h) than conventional CHO cell lines (approximately 20 h). Model monoclonal antibody (IgG1)-producing cells were generated, and the IgG1 concentration of fed-batch culture reached approximately 5 g/L on day 8 in a 200-L bioreactor. The cell bank of CHO-MK cells was prepared as a new host and assessed for contamination by adventitious agents, with the results indicating that it was free from any such contaminants, including infectious viruses. Taking these findings together, this study showed the potential of CHO-MK cells with a shorter doubling time/process time and enhanced productivity in biologics manufacturing.</div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"137 6","pages":"Pages 471-479"},"PeriodicalIF":2.8,"publicationDate":"2024-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140110341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0