Journal of bioscience and bioengineering最新文献

{"title":"Preparation of microcapsules and evaluation of their biocontrol efficacy","authors":"","doi":"10.1016/j.jbiosc.2024.05.007","DOIUrl":"10.1016/j.jbiosc.2024.05.007","url":null,"abstract":"<div><p>In this study, a combination of <span><span>Serratia</span><em> nematophila</em></span> L2 and <span><em>Bacillus</em><em> velezensis</em></span> W24 was used to biocontrol <span><span>Sclerotinia sclerotiorum</span></span>. When the mixed ratio of L2 to W24 was 1:1, the inhibition rate on the growth of <em>S. sclerotiorum</em><span><span> was 88.1 %. To gain a large number of bacteria, the culture medium and conditions were optimized. When the medium formula involved molasses (8.890 g/L), </span>soy<span><span> peptone (6.826 g/L), and NaCl (6.865 g/L), and the culture conditions were 32 °C, inoculum 4%, rotation speed 200 rpm, and pH 7, the maximum amounts of bacterial cells obtained. In order to prepare microcapsules, spray drying conditions were optimized. These conditions included the soluble starch concentration of 30 g/100 mL, the inlet air temperature of 160 °C, and the feed flow rate of 450 mL/h. Under these optimized conditions to prepare microcapsules, the mixed strain (L2 and W24) exhibited a </span>survival rate of 93.9 ± 0.9% and a viable bacterial count of 6.4 × 10</span></span>¹² cfu/g. In addition, microcapsules (GW24Ms) which contained strains L2 and W24 had good storage stability. In the pot experiment, GW24Ms could effectively reduce the disease of soybean plants and the control effect was 88.4%. Thus, the microbial agent represents a promising biocontrol solution for managing <span><em>Sclerotinia</em></span> in soybean.</p></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"138 4","pages":"Pages 328-337"},"PeriodicalIF":2.3,"publicationDate":"2024-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141599922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring gingerol glucosides with enhanced anti-inflammatory activity through a newly identified α-glucosidase (ArG) from Agrobacterium radiobacter DSM 30147","authors":"","doi":"10.1016/j.jbiosc.2024.06.004","DOIUrl":"10.1016/j.jbiosc.2024.06.004","url":null,"abstract":"<div><p><span>Gingerols are phenolic biomedical compounds found in ginger (</span><span><span>Zingiber officinale</span></span>) whose low aqueous solubility limits their medical application. To improve their solubility and produce novel glucosides, an <em>α</em>-glucosidase (glycoside hydrolase) from <span><span>Agrobacterium radiobacter</span></span> DSM 30147 (<em>Ar</em>G) was subcloned, expressed, purified, and then confirmed to have additional <em>α</em>-glycosyltransferase activity. After optimization, the <em>Ar</em>G could glycosylate gingerols into three mono-glucosides based on the length of their acyl side chains. Compound <strong>1</strong> yielded 63.0 %, compound <strong>2</strong> yielded 26.9 %, and compound <strong>3</strong><span> yielded 4.37 %. The production yield of the gingerol glucosides optimally increased in 50 mM phosphate buffer (pH 6) with 50 % (w/v) maltose and 1000 mM Li</span>⁺ at 40 °C for an 24-h incubation. The structures of purified compound <strong>1</strong> and compound <strong>2</strong> were determined as 6-gingerol-5-<em>O</em>-<em>α</em>-glucoside (<strong>1</strong>) and novel 8-gingerol-5-<em>O</em>-<em>α</em>-glucoside (<strong>2</strong><span><span>), respectively, using nucleic magnetic resonance and mass </span>spectral analyses. The aqueous solubility of the gingerol glucosides was greatly improved. Further assays showed that, unusually, 6-gingerol-5-</span><em>O</em>-<em>α</em>-glucoside had 10-fold higher anti-inflammatory activity (IC₅₀ value of 15.3 ± 0.5 μM) than 6-gingerol, while the novel 8-gingerol-5-<em>O</em>-<em>α</em>-glucoside retained 42.7 % activity (IC₅₀ value of 106 ± 4 μM) compared with 8-gingerol. The new <em>α</em>-glucosidase (<em>Ar</em>G) was confirmed to have acidic <em>α</em>-glycosyltransferase activity and could be applied in the production of <em>α</em>-glycosyl derivatives. The 6-gingerol-5-<em>O</em>-<em>α</em>-glucoside can be applied as a clinical drug for anti-inflammatory activity.</p></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"138 3","pages":"Pages 218-224"},"PeriodicalIF":2.3,"publicationDate":"2024-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141599921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cell-penetrating activity of a short-chain ε-poly-l-α-lysine","authors":"","doi":"10.1016/j.jbiosc.2024.06.006","DOIUrl":"10.1016/j.jbiosc.2024.06.006","url":null,"abstract":"<div><p>Bacteria produce polycationic homopoly(amino acid)s, which are characterized by isopeptide backbones. We previously demonstrated that two representative bacterial polycationic isopeptides, ε-poly-<span>l</span>-α-lysine consisting of 25–35 <span>l</span>-α-lysine residues (ε-PαL_25-35) and ε-poly-<span>l</span>-β-lysine consisting of <span>l</span>-β-lysine residues (ε-PβL_4-13), were internalized into mammalian cells by both energy-independent direct penetration and energy-dependent endocytosis/macropinocytosis, and then diffused throughout the cytosol. In this study, we investigated the cell-penetrating activity of an ε-PαL short-chain derivative consisting of 5–14 <span>l</span>-α-lysine residues (ε-PαL_5-14) to gain insight into the relationship between the isopeptide-chain length and the manner of cellular internalization. We prepared a conjugate of ε-PαL_5-14<span> and a fluorescent dye (FAM) by click chemistry, and incubated the resulting polymer, ε-PαL</span>_5-14-FAM, with HeLa cells. Unlike ε-PαL_25-35-FAM, ε-PαL_5-14-FAM was internalized into cells only by energy-dependent endocytosis/macropinocytosis. Furthermore, a high concentration (&gt;50 μM) was required for the internalization events. ε-PαL_5-14 has a chain length almost equal to that of the membrane permeable ε-PβL_4-13, which can enter cells at low concentrations. Considering that the basicity of the β-amino group is higher than that of α-amino acid at physiological pH, ε-PβL is expected to have a greater cell-penetrating capacity than ε-PαL, provided their isopeptide-chain lengths are similar, suggesting that a more extended chain derivative of ε-PβL would be more advantageous for cellular internalization of cargo proteins than ε-PαL_25-35.</p></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"138 3","pages":"Pages 249-253"},"PeriodicalIF":2.3,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141590422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biochemical characterization of l-asparagine synthetase from Streptococcus thermophilus and its application in the enzymatic synthesis of β-aspartyl compounds","authors":"","doi":"10.1016/j.jbiosc.2024.06.001","DOIUrl":"10.1016/j.jbiosc.2024.06.001","url":null,"abstract":"<div><p><span>β-Aspartyl compounds, such as β-aspartyl hydroxamate (serine racemase inhibitor), β-aspartyl-</span><span>l</span>-lysine (moisture retention), and β-aspartyl-<span>l</span><span>-tryptophan (immunomodulator) are physiologically active compounds. There is limited literature on the development of effective methods of production of β-aspartyl compounds. In this study, we describe the biochemical characterization of asparagine synthetase (AS) from </span><span><em>Streptococcus</em><em> thermophilus</em></span> NBRC 13957 (StAS) and the enzymatic synthesis of β-aspartyl compounds using StAS. Recombinant StAS was expressed in <span><em>Escherichia coli</em></span><span> BL21(DE3) and it displayed activity towards hydroxylamine<span><span>, methylamine, </span>ethylamine<span>, and ammonia, as acceptors of the β-aspartyl moiety. StAS exhibited higher activity toward hydroxylamine and ethylamine as acceptor substrates compared with the enzymes from </span></span></span><span><span>Lactobacillus delbrueckii</span></span> NBRC 13953, <span><span>Lactobacillus reuteri</span></span> NBRC 15892, and <em>E. coli</em><span>. The coupling of the synthesis of β-aspartyl compounds by StAS with an ATP-regeneration system using polyphosphate kinase from </span><span><span>Deinococcus</span><em> proteoliticus</em></span> NBRC 101906 displayed an approximately 2.5-fold increase in the production of β-aspartylhydroxamate from 1.06 mM to 2.53 mM after a 76-h reaction.</p></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"138 3","pages":"Pages 206-211"},"PeriodicalIF":2.3,"publicationDate":"2024-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141563469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolic heat based specific growth rate estimators: Does the choice of estimation model influence the state of bioprocesses?","authors":"","doi":"10.1016/j.jbiosc.2024.05.014","DOIUrl":"10.1016/j.jbiosc.2024.05.014","url":null,"abstract":"<div><p>Accurate and reliable estimation of specific growth rate (<span><math><mrow><mi>μ</mi></mrow></math></span><span><span><span><span>) in real-time is pivotal for reliable process monitoring of a bioprocess and subsequent implementation of advanced control strategies. </span>Gibbs free energy dissipation is imminent for any biological system, and the </span>metabolic heat </span>flow measurements (calorimetry) formed the basis for estimating </span><span><math><mrow><mi>μ</mi></mrow></math></span>. However, the rationale behind selecting a suitable <span><math><mrow><mi>μ</mi></mrow></math></span> estimator model based on calorimetric perspective remains unexplored. The present investigation addresses the notion behind the selection of an appropriate estimator for <span><math><mrow><mi>μ</mi></mrow></math></span> and the assessment of the estimator models was illustrated using different types of energy metabolism, namely, high exothermic and low exothermic processes. The results indicated that the <span><math><mrow><mi>μ</mi></mrow></math></span> values from the instantaneous heat flow estimator significantly deviated (10-fold higher) from the offline values for highly exothermic process. Notably, the cumulative heat-based estimator accurately estimated <span><math><mrow><mi>μ</mi></mrow></math></span> values on both types of energy metabolism with performance metrics &lt;0.005 h⁻¹.</p></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"138 3","pages":"Pages 239-248"},"PeriodicalIF":2.3,"publicationDate":"2024-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141563470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bilirubin oxidase expression and activity enhancement from Myrothecium verrucaria in Aspergillus species","authors":"","doi":"10.1016/j.jbiosc.2024.06.002","DOIUrl":"10.1016/j.jbiosc.2024.06.002","url":null,"abstract":"<div><p>We constructed a new <span><span>Aspergillus</span></span><span> expression vector<span><span> (pSENSU2512nid) under the control of the enolase promoter with 12 </span>tandem repeats of </span></span><em>cis</em><span>-acting elements (region III) and the heat shock protein 12 (</span><em>Hsp12</em><span><span><span>) 5′ untranslated region (UTR). </span>Bilirubin </span>oxidase (EC: 1.3.3.5) from </span><span><span>Myrothecium</span><em> verrucaria,</em></span><span><span> which catalyzes the oxidation<span> of bilirubin to </span></span>biliverdin, was overexpressed in </span><span><span>Aspergillus oryzae</span></span> and <em>A</em>. <em>niger</em><span>. The productivity was estimated to be approximately 1.2 g/L in the culture broth, which was approximately 6-fold higher than that of recombinant bilirubin oxidase (BOD) expressed in </span><span><span>Pichia pastoris</span></span> (<span><em>Komagataella</em><em> phaffii</em></span><span><span>). BOD was purified using hydrophobic interaction chromatography, followed by </span>ion exchange chromatography. The specific activity of the purified BOD against 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) substrate was 57.6 U/mg and 66.4 U/mg for </span><em>A. oryzae</em> and <em>A. niger</em>, respectively. <span>l</span>-Ascorbic acid (4 mM) addition and storage under deoxygenated conditions for 3–7 d increased the specific activity of these <span><em>Aspergillus</em></span><span>-expressed BODs approximately 2.3-fold (154.1 U/mg). The BOD specific activity was enhanced by incubation at higher temperature (30–50 °C). Further characterization of the enzyme<span> catalytic efficiency revealed that the </span></span><em>K</em>_m value remained unchanged, whereas the <em>k</em>_cat value improved 3-fold. In conclusion, this high-level of BOD expression meets the requirements for industrial-level production. Additionally, we identified an effective method to enhance the low specific activity during expression, making it advantageous for industrial applications.</p></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"138 3","pages":"Pages 212-217"},"PeriodicalIF":2.3,"publicationDate":"2024-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141537947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating the ability of different chaperones in improving soluble expression of a triple-mutated human interferon gamma in Escherichia coli","authors":"","doi":"10.1016/j.jbiosc.2024.06.005","DOIUrl":"10.1016/j.jbiosc.2024.06.005","url":null,"abstract":"<div><p><span>Human interferon gamma<span> (hIFN-γ) plays a pivotal role as a soluble cytokine with diverse functions in both innate and adaptive immunity. In a previous investigation, we pinpointed three critical amino acid<span> residues, i.e., threonine<span> (T) 27, phenylalanine<span> (F) 29, and leucine (L) 30, on the IFN-γ structure, which are integral to the epitope recognized by anti-IFN-γ autoantibodies. It is crucial to impede the interaction between this epitope and autoantibodies for effective therapy in adult-onset immunodeficiency (AOID). However, the challenge arises from the diminished solubility of the T27AF29L30A mutant in </span></span></span></span></span><em>Escherichia coli</em> BL21(DE3). This study delves into a targeted strategy aimed at improving the soluble expression of IFN-γ T27AF29AL30A. This is achieved through the utilization of five chaperone plasmids: pG-KJE8, pKJE7, pGro7, pG-Tf2, and pTf16. These plasmids, encoding cytoplasmic chaperones, are co-expressed with the IFN-γ mutant in <em>E. coli</em><span> BL21(DE3), and we meticulously analyze the proteins in cell lysate and inclusion bodies using SDS-PAGE and Western blotting<span>. Our findings reveal the remarkable efficacy of pG-KJE8, which houses cytoplasmic chaperones DnaK-DnaJ-GrpE and GroEL-GroES, in significantly enhancing the solubility of IFN-γ T27AF29AL30A. Importantly, this co-expression not only addresses solubility concerns but also preserves the functional dimerized structure, as confirmed by sandwich ELISA. This promising outcome signifies a significant step forward in developing biologic strategies for AOID.</span></span></p></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"138 3","pages":"Pages 232-238"},"PeriodicalIF":2.3,"publicationDate":"2024-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141537948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Contributions of the anaplerotic reaction enzymes pyruvate carboxylase and phosphoenolpyruvate carboxylase to l-lysine production in Corynebacterium glutamicum","authors":"","doi":"10.1016/j.jbiosc.2024.05.015","DOIUrl":"10.1016/j.jbiosc.2024.05.015","url":null,"abstract":"<div><p><span>Anaplerotic reactions catalyzed by pyruvate carboxylase<span> (PC) and phosphoenolpyruvate carboxylase (PEPC) have important roles in the production of </span></span><span>l</span><span>-lysine to replenish oxaloacetic acid (OAA) in </span><span><span>Corynebacterium glutamicum</span></span><span>. However, the relative contributions of these enzymes to </span><span>l</span>-lysine production in <em>C. glutamicum</em><span> are not fully understood. In this study, using a parent strain (P) carrying a feedback inhibition-resistant aspartokinase with the T311I mutation, we constructed a PC gene-deleted mutant strain (PΔPC) and a PEPC gene-deleted mutant strain (PΔPEPC). Although the growth of both mutant strains was comparable to the growth of strain P, the maximum </span><span>l</span>-lysine production in strains PΔPC and PΔPEPC decreased by 14% and 49%, respectively, indicating that PEPC strongly contributed to OAA supply. <span>l</span>-Lysine production in strain PΔPC slightly decreased during the logarithmic phase, while production during the early stationary phase was comparable to production in strain P. By contrast, strain PΔPEPC produced <span>l</span>-lysine in an amount comparable to the production of strain P during the logarithmic phase; <span>l</span>-lysine production after the early stationary phase was completely stopped in strain PΔPEPC. These results indicate that OAA is supplied by both PC and PEPC during the logarithmic phase, while only PEPC can continuously supply OAA after the logarithmic phase.</p></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"138 3","pages":"Pages 225-231"},"PeriodicalIF":2.3,"publicationDate":"2024-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141468222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lowering pH optimum of activity of SshEstI, a slightly alkaliphilic archaeal esterase of the hormone-sensitive lipase family","authors":"","doi":"10.1016/j.jbiosc.2024.05.010","DOIUrl":"10.1016/j.jbiosc.2024.05.010","url":null,"abstract":"<div><p><span><span>SshEstI, a carboxylesterase from the </span>thermoacidophilic archaeon </span><em>Saccharolobus shibatae</em><span><span>, is a member of the hormone-sensitive lipase family that displays slightly alkaliphilic activity with an optimum activity at pH 8.0. In this study, three distinct strategies were explored to confer acidophilic properties to SshEstI. The first strategy involved engineering the oxyanion hole by replacing Gly81 with </span>serine<span> or aspartic acid<span><span>. The G81S mutant showed optimum activity at pH 7.0, whereas the aspartic acid mutant (G81D) rendered the </span>enzyme slightly acidophilic with optimum activity observed at pH 6.0; however, </span></span></span><em>k</em>_cat and <em>k</em>_cat/<em>K</em>_m<span><span> values were reduced by these substitutions. The second strategy involved examining the effects of surfactant additives on the pH-activity profiles of SshEstI. The results showed that cetyltrimethylammonium bromide<span> (CTAB) enhanced wild-type enzyme (WT) activity at acidic pH values. In the presence of 0.1 mM CTAB, G81S and G81D were acidophilic enzymes with optimum activity at pH 6.0 and 4.0, respectively, although their enzyme activities were low. The third strategy involved engineering the active site to resemble that of kumamolisin-As (kuma-As), an acidophilic peptidase of the sedolisin family. The </span></span>catalytic triad of kuma-As was exchanged into SshEstI using site-directed mutagenesis. X-ray crystallographic analysis of the mutants (H274D and H274E) revealed that the potential hydrogen donor–acceptor distances around the active site of WT were fully maintained in these mutants. However, these mutants were inactive at pH 4–8.</span></p></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"138 3","pages":"Pages 188-195"},"PeriodicalIF":2.3,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141450617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Induction of antigen-specific immunity by mesoporous silica nanoparticles incorporating antigen peptides","authors":"","doi":"10.1016/j.jbiosc.2024.05.013","DOIUrl":"10.1016/j.jbiosc.2024.05.013","url":null,"abstract":"<div><p><span><span>Mesoporous silica nanoparticles<span> (MSNs) are physically and chemically stable inorganic nanomaterials<span><span> that have been attracting much attention as carriers for drug delivery systems in the field of </span>nanomedicine. In the present study, we investigated the potential of MSN vaccines that incorporate antigen peptides for use in cancer </span></span></span>immunotherapy. </span><em>In vitro</em> experiments demonstrated that fluorescently labeled MSNs accumulated in a line of mouse dendritic cells (DC2.4 cells), where the particles localized to the cytosol. These observations could suggest that MSNs have potential for use in delivering the loaded molecules into antigen-presenting cells, thereby stimulating the host acquired immune system. <em>In vivo</em><span> experiments demonstrated prolonged survival in mice implanted with ovalbumin<span> (OVA)-expressing lymphoma cells (E.G7-OVA cells) following subcutaneous inoculation with MSNs incorporating OVA antigen peptides. Furthermore, OVA-specific immunoglobulin G antibodies and cytotoxic T lymphocytes were detected in the serum and the spleen cells, respectively, of mice inoculated with an MSN-OVA vaccine, indicating the induction of antigen-specific responses in both the humoral and cellular immune systems. These results suggested that the MSN therapies incorporating antigen peptides may serve as novel vaccines for cancer immunotherapy.</span></span></p></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"138 3","pages":"Pages 254-260"},"PeriodicalIF":2.3,"publicationDate":"2024-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141419266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}