Journal of bioscience and bioengineering最新文献

{"title":"Characterization of unique EDTA-insensitive methylthioalkylmalate synthase from Eutrema japonicum and its potential application in synthetic biology","authors":"Dheeradhach Medhanavyn ,&nbsp;Toshiya Muranaka ,&nbsp;Shuhei Yasumoto","doi":"10.1016/j.jbiosc.2024.02.009","DOIUrl":"10.1016/j.jbiosc.2024.02.009","url":null,"abstract":"<div><p>6-(Methylsulfinyl)hexyl isothiocyanate (6-MSITC), a derivative of glucosinolate with a six-carbon chain, is a compound found in wasabi and has diverse health-promoting properties. The biosynthesis of glucosinolates from methionine depends on a crucial step catalyzed methylthioalkylmalate synthases (MAMs), which are responsible for the generation of glucosinolates with varying chain lengths. In this study, our primary focus was the characterization of two methylthioalkyl malate synthases, MAM1-1 and MAM1-2, derived from <em>Eutrema japonicum</em>, commonly referred to as Japanese wasabi. <em>E</em><em>utrema</em> <em>japonicum</em> MAMs (EjMAMs) were expressed in an <em>E</em><em>scherichia</em> <em>coli</em> expression system, subsequently purified, and <em>in vitro</em> enzymatic activity was assayed. We explored the kinetic properties, optimal pH conditions, and cofactor preferences of EjMAMs and compared them with those of previously documented MAMs. Surprisingly, EjMAM1-2, categorized as a metallolyase family enzyme, displayed 20% of its maximum activity even in the absence of divalent metal cofactors or under high concentrations of EDTA. Additionally, we utilized AlphaFold2 to generate structural homology models of EjMAMs, and used <em>in silico</em> analysis and mutagenesis studies to investigate the key residues participating in catalytic activity. Moreover, we examined <em>in vivo</em> biosynthesis in <em>E. coli</em> containing <em>Arabidopsis thaliana</em> branched-chain amino acid transferase 3 (AtBCAT3) along with AtMAMs or EjMAMs and demonstrated that EjMAM1-2 exhibited the highest conversion rate among those MAMs, converting <span>l</span>-methionine to 2-(2-methylthio) ethyl malate (2-(2-MT)EM). EjMAM1-2 shows a unique property <em>in vitro</em> and highest activity on converting <span>l</span>-methionine to 2-(2-MT)EM <em>in vivo</em> which displays high potential for isothiocyanate biosynthesis in <em>E. coli</em> platform.</p></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"138 1","pages":"Pages 13-20"},"PeriodicalIF":2.8,"publicationDate":"2024-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140756220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overcoming the inhibitory effects of urea to improve the kinetics of microbial-induced calcium carbonate precipitation (MICCP) by Lysinibacillus sphaericus strain MB284","authors":"Seyed Ali Rahmaninezhad ,&nbsp;Mohammad Houshmand ,&nbsp;Amirreza Sadighi ,&nbsp;Kiana Ahmari ,&nbsp;Divya Kamireddi ,&nbsp;Reva M. Street ,&nbsp;Yaghoob (Amir) Farnam ,&nbsp;Caroline L. Schauer ,&nbsp;Ahmad Raeisi Najafi ,&nbsp;Christopher M. Sales","doi":"10.1016/j.jbiosc.2024.03.004","DOIUrl":"10.1016/j.jbiosc.2024.03.004","url":null,"abstract":"<div><p>Among different microbial-induced calcium carbonate precipitation (MICCP) mechanisms utilized for biomineralization, ureolysis leads to the greatest yields of calcium carbonate. Unfortunately, it is reported that urea-induced growth inhibition can delay urea hydrolysis but it is not clear how this affects MICCP kinetics. This study investigated the impact of urea addition on the MICCP performance of <em>Lysinibacillus sphaericus</em> MB284 not previously grown on urea (thereafter named bio-agents), compared with those previously cultured in urea-rich media (20 g/L) (hereafter named bio-agents⁺ or bio-agents-plus). While it was discovered that initial urea concentrations exceeding 3 g/L temporarily hindered cell growth and MICCP reactions for bio-agents, employing bio-agents⁺ accelerated the initiation of bacterial growth by 33% and led to a 1.46-fold increase in the initial yield of calcium carbonate in media containing 20 g/L of urea. The improved tolerance of bio-agents⁺ to urea is attributed to the presence of pre-produced endogenous urease, which serves to reduce the initial urea concentration, alleviate growth inhibition, and expedite biomineralization. Notably, elevating the initial concentration of bio-agents⁺ from OD₆₀₀ of 0.01 to 1, housing a higher content of endogenous urease, accelerated the initiation of MICCP reactions and boosted the ultimate yield of biomineralization by 2.6 times while the media was supplemented with 20 g/L of urea. These results elucidate the advantages of employing bio-agents⁺ with higher initial cell concentrations to successfully mitigate the temporary inhibitory effects of urea on biomineralization kinetics, offering a promising strategy for accelerating the production of calcium carbonate for applications like bio self-healing of concrete.</p></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"138 1","pages":"Pages 63-72"},"PeriodicalIF":2.8,"publicationDate":"2024-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140775707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent advances in plant-based bioproduction","authors":"Kazuhito Fujiyama ,&nbsp;Toshiya Muranaka ,&nbsp;Atsushi Okazawa ,&nbsp;Hikaru Seki ,&nbsp;Goro Taguchi ,&nbsp;Shuhei Yasumoto","doi":"10.1016/j.jbiosc.2024.01.007","DOIUrl":"10.1016/j.jbiosc.2024.01.007","url":null,"abstract":"<div><p>Unable to move on their own, plants have acquired the ability to produce a wide variety of low molecular weight compounds to survive against various stresses. It is estimated that there are as many as one million different kinds. Plants also have the ability to accumulate high levels of proteins. Although plant-based bioproduction has traditionally relied on classical tissue culture methods, the attraction of bioproduction by plants is increasing with the development of omics and bioinformatics and other various technologies, as well as synthetic biology. This review describes the current status and prospects of these plant-based bioproduction from five advanced research topics, (i) <em>de novo</em> production of plant-derived high value terpenoids in engineered yeast, (ii) biotransformation of plant-based materials, (iii) genome editing technology for plant-based bioproduction, (iv) environmental effect of metabolite production in plant factory, and (v) molecular pharming.</p></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"138 1","pages":"Pages 1-12"},"PeriodicalIF":2.8,"publicationDate":"2024-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140787849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characteristics of kopyor coconut (Cocos nucifera L.) using sensory analysis and metabolomics-based approach","authors":"Mercy Bientri Yunindanova ,&nbsp;Sastia Prama Putri ,&nbsp;Hengky Novarianto ,&nbsp;Eiichiro Fukusaki","doi":"10.1016/j.jbiosc.2024.02.008","DOIUrl":"10.1016/j.jbiosc.2024.02.008","url":null,"abstract":"<div><p>Kopyor is a coconut with unique characteristics from Indonesia, one of the largest coconut producers in the world. Kopyor is an edible mature coconut with soft endosperm. Although this fruit is one of the most popular coconuts in the world, there are limited studies on its properties, including its sensory attributes and metabolite profiles. This study investigates the characteristics of kopyor using sensory evaluation, a widely targeted metabolomics approach, and multivariate analysis. The liquid (water) and solid (flesh) endosperms were collected as the samples. The results showed that kopyor has characteristics that distinguish it from normal mature and young coconuts. Kopyor water has a milky, creamy, nutty, bitter, and astringent taste with an oily aftertaste and mouthfeel. Kopyor flesh is soft and moist and gives a sandy mouth feel. This study analyzed the sensory attributes of the kopyor endosperm for the first time and compared it with those of normal mature and young coconuts. A gas chromatography mass spectrometry analysis showed that kopyor contained wider variety of metabolites than normal coconuts of the same age. Based on the differential analysis and orthogonal projections to latent structures-regression, kopyor water was characterized by the accumulation of flavor-related metabolites, such as amino acids and organic acids, which contributed to its sensory complexity. This study solidified the effects of maturation and endosperm type on metabolite accumulation in kopyor endosperm. This pioneering information will lead to the future use of kopyor and other unique coconuts worldwide for food, contributing to the sustainability of the coconut industry.</p></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"138 1","pages":"Pages 44-53"},"PeriodicalIF":2.8,"publicationDate":"2024-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140759897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bone morphogenetic protein signaling inhibitor improves differentiation and function of 3D muscle construct fabricated using C2C12","authors":"Ran Ding ,&nbsp;Yuan Xi ,&nbsp;Akira Ito ,&nbsp;Kazunori Shimizu ,&nbsp;Eiji Nagamori ,&nbsp;Hideaki Fujita ,&nbsp;Takuo Kawamoto ,&nbsp;Masanobu Horie","doi":"10.1016/j.jbiosc.2024.03.005","DOIUrl":"10.1016/j.jbiosc.2024.03.005","url":null,"abstract":"<div><p>Functional tissue-engineered artificial skeletal muscle tissue has great potential for pharmacological and academic applications. This study demonstrates an <em>in vitro</em> tissue engineering system to construct functional artificial skeletal muscle tissues using self-organization and signal inhibitors. To induce efficient self-organization, we optimized the substrate stiffness and extracellular matrix (ECM) coatings. We modified the tissue morphology to be ring-shaped under optimized self-organization conditions. A bone morphogenetic protein (BMP) inhibitor was added to improve overall myogenic differentiation. This supplementation enhanced the myogenic differentiation ratio and myotube hypertrophy in two-dimensional cell cultures. Finally, we found that myotube hypertrophy was enhanced by a combination of self-organization with ring-shaped tissue and a BMP inhibitor. BMP inhibitor treatment significantly improved myogenic marker expression and contractile force generation in the self-organized tissue. These observations indicated that this procedure may provide a novel and functional artificial skeletal muscle for pharmacological studies.</p></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"137 6","pages":"Pages 480-486"},"PeriodicalIF":2.8,"publicationDate":"2024-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140779158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of microbial dynamics in the soybean root-associated environments from community to single-cell levels","authors":"Masako Kifushi ,&nbsp;Yohei Nishikawa ,&nbsp;Masahito Hosokawa ,&nbsp;Keigo Ide ,&nbsp;Masato Kogawa ,&nbsp;Toyoaki Anai ,&nbsp;Haruko Takeyama","doi":"10.1016/j.jbiosc.2024.02.007","DOIUrl":"10.1016/j.jbiosc.2024.02.007","url":null,"abstract":"<div><p>Plant root-associated environments such as the rhizosphere, rhizoplane, and endosphere, are notably different from non-root-associated soil environments. However, the microbial dynamics in these spatially divided compartments remain unexplored. In this study, we propose a combinational analysis of single-cell genomics with 16S rRNA gene sequencing. This method enabled us to understand the entire soil microbiome and individual root-associated microorganisms. We applied this method to soybean microbiomes and revealed that their composition was different between the rhizoplane and rhizosphere in the early growth stages, but became more similar as growth progressed. In addition, a total of 610 medium- to high-quality single-amplified genomes (SAGs) were acquired, including plant growth-promoting rhizobacteria (PGPR) candidates while genomes with high GC content tended to be missed by SAGs. The whole-genome analyses of the SAGs suggested that rhizoplane-enriched <em>Flavobacterium</em> solubilizes organophosphate actively and <em>Bacillus</em> colonizes roots more efficiently. Single-cell genomics, together with 16S rRNA gene sequencing, enabled us to connect microbial taxonomy and function, and assess microorganisms at a strain resolution even in the complex soil microbiome.</p></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"137 6","pages":"Pages 429-436"},"PeriodicalIF":2.8,"publicationDate":"2024-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140790684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and characterization of novel N-acylhomoserine lactonase from nonpathogenic Allorhizobium vitis, a candidate for biocontrol agent","authors":"Tomohiro Morohoshi ,&nbsp;Koki Hirose ,&nbsp;Nobutaka Someya","doi":"10.1016/j.jbiosc.2024.03.001","DOIUrl":"10.1016/j.jbiosc.2024.03.001","url":null,"abstract":"<div><p>Some strains of nonpathogenic <em>Allorhizobium vitis</em> can control crown gall disease in grapevines caused by pathogenic <em>A. vitis</em> and are considered candidates for biocontrol agents. Many plant pathogenic bacteria regulate the expression of their virulence genes via quorum sensing using <em>N</em>-acylhomoserine lactone (AHL) as a signaling compound. The eight nonpathogenic <em>A. vitis</em> strains used in this study showed AHL-degrading activity. The complete genome sequence of <em>A. vitis</em> MAFF 212306 contained three AHL lactonase gene homologs. When these genes were cloned and transformed into <em>Escherichia coli</em> DH5α, <em>E. coli</em> harboring the <em>aiiV</em> gene (RvVAR031_27660) showed AHL-degrading activity. The <em>aiiV</em> coding region was successfully amplified by polymerase chain reaction from the genomes of all eight strains of nonpathogenic <em>A. vitis</em>. Purified His-tagged AiiV exhibited AHL lactonase activity by hydrolyzing the lactone ring of AHL. AiiV had an optimal temperature of approximately 30 °C; however, its thermostability decreased above 40 °C. When the AiiV-expressing plasmid was transformed into <em>Pectobacterium carotovorum</em> subsp. <em>carotovorum</em> NBRC 3830, AHL production by NBRC 3830 decreased below the detection limit, and its maceration activity, which was controlled by quorum sensing, almost disappeared. These results suggest the potential use of AHL-degrading nonpathogenic <em>A. vitis</em> for the inhibition of crown gall disease in grapevines and other plant diseases controlled by quorum sensing.</p></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"137 6","pages":"Pages 437-444"},"PeriodicalIF":2.8,"publicationDate":"2024-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140860259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interpenetrating gelatin/alginate mixed hydrogel: The simplest method to prepare an autoclavable scaffold","authors":"Hideki Mori,&nbsp;Yaya Taketsuna,&nbsp;Kae Shimogama,&nbsp;Koki Nishi,&nbsp;Masayuki Hara","doi":"10.1016/j.jbiosc.2024.01.015","DOIUrl":"10.1016/j.jbiosc.2024.01.015","url":null,"abstract":"<div><p>The choice of sterilization method for hydrogels used for cell culture influences the ease of preparing the gel. We prepared interpenetrating gelatin/calcium alginate hydrogels containing 1% (w/v) alginate and 1–16% (w/v) gelatin by molding with the mixture of gelatin/sodium alginate solution, followed by the addition of calcium ions by incubation in calcium chloride solution. It is the simplest method to prepare autoclavable gelatin/sodium hydrogel. We measured various properties of the hydrogels including volume, Young's modulus in the compression test, storage modulus, and loss modulus in the dynamic viscoelasticity measurement. The gelatin/alginate hydrogel can be easily fabricated into any shape by this method. After autoclave treatment, the hydrogel was shrunk to smaller than the original shape in similar figures. The shape of the gelatin/alginate hydrogel can be designed into any shape with the reduction ratio of the volume. Human osteosarcoma (HOS) cells adhered to the gelatin/alginate hydrogel and then proliferated. Gelatin/calcium alginate hydrogels with a high concentration are considered to be autoclavable culture substrates because of their low deformation and gelatin elution rate after autoclaving and the high amount of cells attached to the hydrogels.</p></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"137 6","pages":"Pages 463-470"},"PeriodicalIF":2.8,"publicationDate":"2024-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140764538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Non-pathogenic Heyndrickxia coagulans (Bacillus coagulans) 29-2E inhibits the virulence of pathogenic Salmonella Typhimurium by quorum-sensing regulation","authors":"Masafumi Noda ,&nbsp;Shino Noguchi ,&nbsp;Narandalai Danshiitsoodol ,&nbsp;Toshinori Hara ,&nbsp;Masanori Sugiyama","doi":"10.1016/j.jbiosc.2024.03.002","DOIUrl":"10.1016/j.jbiosc.2024.03.002","url":null,"abstract":"<div><p>Bacteria produce and release small signal molecules, autoinducers, as an indicator of their cell density. The system, called a quorum-sensing (QS) system, is used to control not only virulence factors but also antibiotic production, sporulation, competence, and biofilm formation in bacteria. Different from antibiotics, QS inhibitors are expected to specifically repress the virulence factors in pathogenic bacteria without inhibiting growth or bactericidal effects. Therefore, since QS inhibitors have little risk of antibiotic-resistant bacteria emergence, they have been proposed as promising anti-bacterial agents. In the present study, we aimed to find new QS inhibitors that prohibit the signaling cascade of autoinducer 3 (AI-3) recognized by a QseCB two-component system that regulates some virulence factors of pathogens, such as enterohemorrhagic <em>Escherichia coli</em> (EHEC) and <em>Salmonella enterica</em> subsp. <em>enterica</em> serovar Typhimurium. We have established the method for QS-inhibitor screening using a newly constructed plasmid pLES-AQSA. <em>E. coli</em> DH5α transformed with the pLES-AQSA can produce β-galactosidase that converts 5-bromo-4-chloro-3-indolyl β-d-galactopyranoside (X-gal) into blue pigment (5-bromo-4-chloro-indoxyl) under the control of the QseCB system. By screening, <em>Heyndrickxia coagulans</em> (formerly <em>Bacillus coagulans</em>) 29-2E was found to produce an exopolysaccharide (EPS)-like water-soluble polymer that prohibits QseCB-mediated β-galactosidase production without antibacterial activities. Further, the simultaneous injection of the 29-2E strain significantly improves the survival rate of <em>Salmonella</em> Typhimurium-infected silkworm larvae (from 0% to 83.3%), suggesting that the substance may be a promising inhibitor against the virulence of pathogens without risk of the emergence of antibiotic-resistant bacteria.</p></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"137 6","pages":"Pages 445-452"},"PeriodicalIF":2.8,"publicationDate":"2024-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140326605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physiological evaluation of yeast strains under anaerobic conditions using glucose, fructose, or sucrose as the carbon source","authors":"Cinthia Aparecida de Andrade Silva ,&nbsp;Marta Ligia Oka ,&nbsp;Pedro Garcia Pereira da Silva ,&nbsp;Janaina Mayumi Honma ,&nbsp;Rodrigo Simões Ribeiro Leite ,&nbsp;Gustavo Graciano Fonseca","doi":"10.1016/j.jbiosc.2024.02.003","DOIUrl":"10.1016/j.jbiosc.2024.02.003","url":null,"abstract":"<div><p>The aim of this study was to evaluate the physiology of 13 yeast strains by assessing their kinetic parameters under anaerobic conditions. They included <em>Saccharomyces cerevisiae</em> CAT-1 and 12 isolated yeasts from different regions in Brazil. The study aimed to enhance understanding of the metabolism of these strains for more effective applications. Measurements included quantification of sugars, ethanol, glycerol, and organic acids. Various kinetic parameters were analyzed, such as specific substrate utilization rate (<em>q</em>_S), maximum specific growth rate (<em>μ</em>_max), doubling time, biomass yield, product yield, maximum cell concentration, ethanol productivity (<em>P</em>_Eth), biomass productivity, and CO₂ concentration. <em>S. cerevisiae</em> CAT-1 exhibited the highest values in glucose for <em>μ</em>_max (0.35 h⁻¹), <em>q</em>_S (3.06 h⁻¹), and <em>P</em>_Eth (0.69 gEth L⁻¹ h⁻¹). <em>Candida parapsilosis</em> Recol 37 did not fully consume the substrate. In fructose, <em>S. cerevisiae</em> CAT-1 stood out with higher values for <em>μ</em>_max (0.25 h⁻¹), <em>q</em>_S (2.24 h⁻¹), and <em>P</em>_Eth (0.60 gEth L⁻¹ h⁻¹). <em>Meyerozyma guilliermondii</em> Recol 09 and <em>C. parapsilosis</em> Recol 37 had prolonged fermentation times and residual substrate. In sucrose, only <em>S. cerevisiae</em> CAT-1, <em>S. cerevisiae</em> BB9, and <em>Pichia kudriavzevii</em> Recol 39 consumed all the substrate, displaying higher <em>P</em>_Eth (0.72, 0.51, and 0.44 gEth L⁻¹ h⁻¹, respectively) compared to other carbon sources.</p></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"137 6","pages":"Pages 420-428"},"PeriodicalIF":2.8,"publicationDate":"2024-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140140189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}