Journal of bioscience and bioengineering最新文献

In-situ collagen mineralization modulates metastatic properties of breast cancer cells. 原位胶原矿化可调节乳腺癌细胞的转移特性。

IF 2.3 4区生物学

Journal of bioscience and bioengineering Pub Date : 2024-11-22 DOI: 10.1016/j.jbiosc.2024.07.010

Jaya Thilakan, Sudhir Kumar Goel, Neha Arya

{"title":"In-situ collagen mineralization modulates metastatic properties of breast cancer cells.","authors":"Jaya Thilakan, Sudhir Kumar Goel, Neha Arya","doi":"10.1016/j.jbiosc.2024.07.010","DOIUrl":"https://doi.org/10.1016/j.jbiosc.2024.07.010","url":null,"abstract":"Bone metastasis is the leading cause of morbidity and mortality in advanced-stage breast cancer patients. While most studies focus on the cellular and genetic factors associated with breast cancer metastasis, the role of the extracellular matrix (ECM) of bone in breast cancer metastasis remains elusive. In this study, we recapitulated the bone microenvironment using in-situ mineralized collagen type-I hydrogels and utilized them to understand breast cancer metastasis. Our results indicated successful mineralization of collagen type-I based hydrogels in the presence of serum proteins, which increased as a function of time. There was no difference in the adhesion of breast cancer cells seeded on collagen and mineralized collagen surfaces. However, there was a marked reduction in cell proliferation, down-regulation of various metastatic markers, and decreased migratory phenotype with a concomitant increase in cleaved caspase-3 on mineralized collagen compared to collagen hydrogels. In conclusion, our results suggest an inverse relationship between bone mineralization and the metastatic propensity of breast cancer cells. We further speculate the role of other factors in the skeletal ecosystem for mediating preferential homing of breast cancer cells to the bone microenvironment.","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142695515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and characterization of a circular bacteriocin, garvicin SC, a novel garvicin ML variant, produced by Lactococcus garvieae ABG0038. 加维氏乳球菌（Lactococcus garvieae）ABG0038 产生的一种环状细菌毒素加维素 SC（一种新型加维素 ML 变体）的鉴定和特征描述。

IF 2.3 4区生物学

Journal of bioscience and bioengineering Pub Date : 2024-11-15 DOI: 10.1016/j.jbiosc.2024.10.008

Yumi Komori, Naoya Ozawa, Hiroshi Kuwahara, Takeshi Zendo, Mikio Aoki

引用次数: 0

Comparison of a new peak detection function for selecting a phase-appropriate multi-attribute method system. 用于选择相位合适的多属性方法系统的新峰值检测函数的比较。

IF 2.3 4区生物学

Journal of bioscience and bioengineering Pub Date : 2024-11-09 DOI: 10.1016/j.jbiosc.2024.10.005

Eriko Numao, Kumi Yanagisawa, Yuki Yagi, Daisuke Tsuchida, Katsuyoshi Yamazaki

{"title":"Comparison of a new peak detection function for selecting a phase-appropriate multi-attribute method system.","authors":"Eriko Numao, Kumi Yanagisawa, Yuki Yagi, Daisuke Tsuchida, Katsuyoshi Yamazaki","doi":"10.1016/j.jbiosc.2024.10.005","DOIUrl":"https://doi.org/10.1016/j.jbiosc.2024.10.005","url":null,"abstract":"The multi-attribute method (MAM) has been recognized as an optimal tool for quality control in biotherapeutics. New peak detection (NPD) is one of the functions of MAM for detecting unexpected differences in samples and is an essential feature required for replacing conventional methods with MAM. Not only used for release and stability testing, NPD is also considered valuable for evaluating comparability and identifying product quality attributes in the research phase. Although many researchers consider the processing parameter the key to NPD, the details of the decision-making process are unclear. Besides specific instruments and software packages has been used almost exclusively, yet the differences in NPD function between other choices have not been confirmed. Thus, this research aimed to confirm the applicability of our original decision-making approach for NPD processing parameters using two different systems. After optimization for each, under a condition that detected crucial differences and did not return false positives, they differed in the reproducibility of the results. To our knowledge, this was the first time the comparison of NPD results of different systems has been published, and the eligibility of processing methods was evaluated in light of the equivalency of conventional methods' detectability. The findings suggested that the capability of NPD is determined not only by the instrument's resolution but also by the software's capability. Our approach for optimizing the NPD processing parameter is deemed widely applicable and practical in developing therapeutic proteins. The revealed difference will help us select the fit-for-purpose system.","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142621135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plasma activated medium suppresses proliferation and migration of human lung cancer cells by regulating PI3K/AKT-Wnt signaling pathway. 血浆活化培养基通过调节 PI3K/AKT-Wnt 信号通路抑制人肺癌细胞的增殖和迁移

IF 2.3 4区生物学

Journal of bioscience and bioengineering Pub Date : 2024-11-07 DOI: 10.1016/j.jbiosc.2024.10.002

Zhidan Sun, Chenglong Ding, Yuhan Wang, Han Zhou, Wencheng Song

{"title":"Plasma activated medium suppresses proliferation and migration of human lung cancer cells by regulating PI3K/AKT-Wnt signaling pathway.","authors":"Zhidan Sun, Chenglong Ding, Yuhan Wang, Han Zhou, Wencheng Song","doi":"10.1016/j.jbiosc.2024.10.002","DOIUrl":"https://doi.org/10.1016/j.jbiosc.2024.10.002","url":null,"abstract":"The main causes of high mortality in lung cancer patients are the malignant growth and migration of cancer cells. This study aims to investigate the underlying mechanisms of low-temperature plasma-activated medium (PAM) treating human lung cancer (HLC). Changes in the levels of reactive oxygen and nitrogen species both inside and outside the cells were evaluated. Our results showed that prolonged PAM exposure decreased cell viability, raised intracellular reactive oxygen species levels, and hindered cell migration while reducing mitochondrial membrane potential. Protein analysis revealed PAM increased GSK-3β and p-β-catenin expression but decreased PI3K, AKT, p-AKT, p-GSK-3β, Wnt, and β-catenin levels, thereby inhibiting the epithelial-mesenchymal transition. These findings suggest PAM suppresses HLC cells proliferation and migration by blocking the PI3K/AKT-Wnt pathway. The study will provide a valuable theoretical basis for future low-temperature plasma treatment, thereby improving the survival rates and prognosis of lung cancer.","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142621139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular mechanisms of complex-type N-glycan breakdown and metabolism by the human intestinal bacterium Barnesiella intestinihominis. 人类肠道细菌 Barnesiella intestinihominis 分解和代谢复合型 N-糖的分子机制。

IF 2.3 4区生物学

Journal of bioscience and bioengineering Pub Date : 2024-11-06 DOI: 10.1016/j.jbiosc.2024.10.006

Kanako Doi, Kazuki Mori, Misaki Komatsu, Akari Shinoda, Kosuke Tashiro, Yujiro Higuchi, Jiro Nakayama, Kaoru Takegawa

{"title":"Molecular mechanisms of complex-type N-glycan breakdown and metabolism by the human intestinal bacterium Barnesiella intestinihominis.","authors":"Kanako Doi, Kazuki Mori, Misaki Komatsu, Akari Shinoda, Kosuke Tashiro, Yujiro Higuchi, Jiro Nakayama, Kaoru Takegawa","doi":"10.1016/j.jbiosc.2024.10.006","DOIUrl":"https://doi.org/10.1016/j.jbiosc.2024.10.006","url":null,"abstract":"Intestinal bacteria play a crucial role in human health, for example, by maintaining immune and metabolic homeostasis and protecting against pathogens. Survival in the human intestine depends on the bacterium's ability to utilize complex carbohydrates. Some species are known to use host-derived glycans; for example, Bifidobacteria can utilize O-glycan of mucin. However, there are few studies on intestinal bacteria utilizing host-derived N-glycan. Here, we identified the mechanism underlying the breakdown and utilization of complex-type N-glycan by the human intestinal bacterium Barnesiella intestinihominis. A growth assay showed that B. intestinihominis can utilize complex-type N-glycan as a carbon source, while RNA-seq analysis identified enzymes and transporters involved in the mechanism of N-glycan breakdown. In particular, the expression of three genes encoding glycoside hydrolase 85 endo-β-N-acetylglucosaminidase (endo-BIN1, endo-BIN2, and endo-BIN3) rose markedly in bacterial cells cultured in complex-type N-glycoprotein medium. We also found that the susC and susD genes, encoding the SusC/SusD membrane complex, form a gene cluster with endo-BIN genes, suggesting that SusC/SusD is involved in transportation of the glycan into the cell. Other genes encoding exo-type glycoside hydrolase enzymes showed elevated expression in cells grown in complex-type N-glycoprotein medium, suggesting that these enzymes function in further degradation of glycan for metabolism by the bacterium. Collectively, these findings suggest the survival strategy of an intestinal bacterium that has a unique metabolic pathway to use host-derived complex-type N-glycan as a nutrient.","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142604889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Geobacter sulfurreducens strain 60473, a potent bioaugmentation agent for improving the performances of bioelectrochemical systems. Geobacter sulfurreducens 菌株 60473，一种用于提高生物电化学系统性能的强效生物增强剂。

IF 2.3 4区生物学

Journal of bioscience and bioengineering Pub Date : 2024-11-06 DOI: 10.1016/j.jbiosc.2024.10.007

Tomoka Harada, Yohei Yamada, Mizuki Toda, Yuki Takamatsu, Keisuke Tomita, Kengo Inoue, Atsushi Kouzuma, Kazuya Watanabe

{"title":"Geobacter sulfurreducens strain 60473, a potent bioaugmentation agent for improving the performances of bioelectrochemical systems.","authors":"Tomoka Harada, Yohei Yamada, Mizuki Toda, Yuki Takamatsu, Keisuke Tomita, Kengo Inoue, Atsushi Kouzuma, Kazuya Watanabe","doi":"10.1016/j.jbiosc.2024.10.007","DOIUrl":"https://doi.org/10.1016/j.jbiosc.2024.10.007","url":null,"abstract":"Bioaugmentation with electrochemically active bacteria (EAB) has been suggested useful for improving the performance of bioelectrochemical systems (BESs) for sustainable energy generation, while its success is dependent on EAB introduced into the systems. Here we report on the isolation of a novel EAB, Geobacter sulfurreducens strain 60473, from microbes that colonized on an anode of a sediment microbial fuel cell. This strain is highly adhesive to graphite electrodes, forms dense biofilms on electrode surfaces, and generates high current densities in BESs. When microbial electrolysis cells (MECs) inoculated with paddy-field soil and fed starch as the major organic substrate were augmented with strain 60473, Geobacter bacteria predominantly colonized on anodes, and MEC performances, including current generation, hydrogen production and organics removal, were substantially improved compared to non-bioaugmented controls. Results suggest that bioaugmentation with electrode-adhesive EAB, such as strain 60473, is a promising approach for improving the performance of BESs, including MECs treating fermentable organics and biomass wastes.","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142604875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization and application of Lachancea thermotolerans isolates for sake brewing. 用于清酒酿造的 Lachancea thermotolerans 分离物的特征和应用。

IF 2.3 4区生物学

Journal of bioscience and bioengineering Pub Date : 2024-11-02 DOI: 10.1016/j.jbiosc.2024.10.004

Miyu Nakatani, Rina Ohtani, Kiwamu Umezawa, Taiyo Uchise, Yoshifumi Matsuo, Yasuhisa Fukuta, Eri Obata, Aruma Katabuchi, Kento Kizaki, Hana Kitazume, Masataka Ohashi, Katsuki Johzuka, Atsushi Kurata, Koichi Uegaki

{"title":"Characterization and application of Lachancea thermotolerans isolates for sake brewing.","authors":"Miyu Nakatani, Rina Ohtani, Kiwamu Umezawa, Taiyo Uchise, Yoshifumi Matsuo, Yasuhisa Fukuta, Eri Obata, Aruma Katabuchi, Kento Kizaki, Hana Kitazume, Masataka Ohashi, Katsuki Johzuka, Atsushi Kurata, Koichi Uegaki","doi":"10.1016/j.jbiosc.2024.10.004","DOIUrl":"https://doi.org/10.1016/j.jbiosc.2024.10.004","url":null,"abstract":"Non-conventional yeasts are increasingly being used in the production of fermented beverages owing to their ability to create unique and high-quality products. The yeast Lachancea thermotolerans is of great industrial significance, particularly in the production of l(+)-lactic acid, which is beneficial for acidifying wine, beer, and potentially sake. To explore its potential in sake brewing, three L. thermotolerans strains were isolated from natural environments and their physiological and fermentative characteristics were examined. The isolates surpassed the L. thermotolerans type strain (NBRC 1985) in lactic acid production under various culture conditions and exhibited comparable growth rates to that of Saccharomyces cerevisiae at 15-20 °C. Sake brewing tests using these isolates yielded approximately 3500 ppm of lactic acid, with a slightly lower production of aroma components compared to that produced by sake yeast, and an ethanol content of approximately 11-12 % was obtained. Reverse transcription-quantitative polymerase chain reaction revealed variable expression in putative lactate dehydrogenase genes depending on the culture conditions. Our findings suggest that L. thermotolerans strains can be used in sake brewing to produce unique sake.","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142568685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metagenomic profiling of antibiotic resistance genes and their associations with the bacterial community along the Kanda River, an urban river in Japan. 日本城市河流神田川沿岸抗生素耐药性基因及其与细菌群落关系的元基因组分析。

IF 2.3 4区生物学

Journal of bioscience and bioengineering Pub Date : 2024-11-01 DOI: 10.1016/j.jbiosc.2024.09.006

Chang Xiao, Keigo Ide, Hiroko Matsunaga, Masato Kogawa, Ryota Wagatsuma, Haruko Takeyama

{"title":"Metagenomic profiling of antibiotic resistance genes and their associations with the bacterial community along the Kanda River, an urban river in Japan.","authors":"Chang Xiao, Keigo Ide, Hiroko Matsunaga, Masato Kogawa, Ryota Wagatsuma, Haruko Takeyama","doi":"10.1016/j.jbiosc.2024.09.006","DOIUrl":"https://doi.org/10.1016/j.jbiosc.2024.09.006","url":null,"abstract":"Antibiotic resistance genes (ARGs) present in urban rivers have the potential to disseminate antibiotic-resistant bacteria into other environments, posing significant threats to both ecological and public health. Although metagenomic analyses have been widely employed to detect ARGs in rivers, our understanding of their dynamics across different seasons in diverse watersheds remains limited. In this study, we performed a comprehensive genomic analysis of the Kanda River in Japan at 11 sites from upstream to estuary throughout the year to assess the spread of ARGs and their associations with bacterial communities. Analysis of 110 water samples using the 16S rRNA gene revealed variations in bacterial composition corresponding to seasonal changes in environmental parameters along the river. Shotgun metagenomics-based profiling of ARGs in 44 water samples indicated higher ARG abundance downstream, particularly during the summer. Weighted gene co-expression network analysis (WGCNA) linking bacterial lineages and ARGs revealed that 12 ARG subtypes co-occurred with 128 amplicon sequence variants (ASVs). WGCNA suggested potential hosts for ErmB, ErmF, ErmG, tetQ, tet (W/N/W), aadA2, and adeF, including gut-associated bacteria (e.g., Prevotella, Bacteroides, Arcobacter) and indigenous aquatic microbes (e.g., Limnohabitans and C39). In addition, Pseudarcobacter (a later synonym of Arcobater) was identified as a host for adeF, which was also confirmed by single cell genomics. This study shows that ARG distribution in urban rivers is affected by seasonal and geographical factors and demonstrates the importance of monitoring rivers using multiple types of genome sequencing, including 16S rRNA gene sequencing, metagenomics, and single cell genomics.","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142564516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mass spectrometry imaging of gamma-aminobutyric acid and glutamic acid decarboxylase reactions at various stages of banana ripening. 香蕉成熟不同阶段γ-氨基丁酸和谷氨酸脱羧酶反应的质谱成像。

IF 2.3 4区生物学

Journal of bioscience and bioengineering Pub Date : 2024-10-30 DOI: 10.1016/j.jbiosc.2024.10.001

Shiho Ishimoto, Eiichiro Fukusaki, Shuichi Shimma

{"title":"Mass spectrometry imaging of gamma-aminobutyric acid and glutamic acid decarboxylase reactions at various stages of banana ripening.","authors":"Shiho Ishimoto, Eiichiro Fukusaki, Shuichi Shimma","doi":"10.1016/j.jbiosc.2024.10.001","DOIUrl":"https://doi.org/10.1016/j.jbiosc.2024.10.001","url":null,"abstract":"Banana is the fourth most consumed crop worldwide, and its high economic value and health benefits have made it very popular. Bananas are climacteric fruits that ripen after harvesting. It has been reported that the endogenous substances in bananas change significantly during the ripening process. This study focused on levels of gamma-aminobutyric acid (GABA) and glutamic acid decarboxylase (GAD), an enzyme that catalyzes the synthesis of GABA, which reportedly fluctuates during the ripening stage. Previous studies have shown that GAD expression is associated with banana ripening; however, changes in its distribution during ripening have not been verified. This study aimed to clarify the relationship between GABA and GAD during ripening of ethylene-treated bananas. Visualization of the localization of endogenous GABA and GAD was performed using mass spectrometry imaging. To visualize GAD reaction, a glutamate-d3 (labeled substrate) was supplied to the sample, and a GABA-d3 (labeled product) was regarded as the localization of the enzymatic reaction. Liquid chromatography-mass spectrometry was also used to confirm the amount of GABA and activity of the GAD. This will allow us to clarify the direct relationship between GABA and GAD and to understand the role of the GAD reaction in phytohormones.","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142557905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Aromatic residues in the oligonucleotide binding domain are essential to the function of the single-stranded DNA binding protein of Helicobacter pylori. 寡核苷酸结合域中的芳香残基对幽门螺旋杆菌单链 DNA 结合蛋白的功能至关重要。

IF 2.3 4区生物学

Journal of bioscience and bioengineering Pub Date : 2024-10-25 DOI: 10.1016/j.jbiosc.2024.09.003

Mon-Juan Lee, Li-Kun Huang, Wen-Hsin Huang, Po-Yu Chan, Zi-Sin Yang, Ching-Ming Chien, Ching-Chang Chieng, Haimei Huang

{"title":"Aromatic residues in the oligonucleotide binding domain are essential to the function of the single-stranded DNA binding protein of Helicobacter pylori.","authors":"Mon-Juan Lee, Li-Kun Huang, Wen-Hsin Huang, Po-Yu Chan, Zi-Sin Yang, Ching-Ming Chien, Ching-Chang Chieng, Haimei Huang","doi":"10.1016/j.jbiosc.2024.09.003","DOIUrl":"https://doi.org/10.1016/j.jbiosc.2024.09.003","url":null,"abstract":"Single-stranded DNA-binding protein (SSB) is essential to DNA replication, DNA repair, and homologous genetic recombination. Our previous study on the crystal structure of a C-terminally truncated SSB from Helicobacter pylori, HpSSBc, in complex with single-stranded DNA (ssDNA) suggests that several aromatic residues, including Phe37, Phe50, Phe56, and Trp84, were involved in ssDNA binding. To investigate the importance of these aromatic residues, the binding activity of four site-directed HpSSB mutants, including F37A HpSSB, F50A HpSSB, F56A HpSSB, and W84A HpSSB, was compared to that of wild-type HpSSB and HpSSBc by means of electrophoresis mobility shift assay (EMSA), tryptophan quenching fluorescence titration, and surface plasmon resonance (SPR). Molecular docking and molecular dynamic (MD) simulation of a F37A and a quadruple mutation model of HpSSBc support that the ssDNA-HpSSBc complex was destabilized when either one or four of the aromatic residues were mutated. The findings of this study suggest that mutation of the phenylalanine and tryptophan residues within the oligonucleotide-binding domain significantly diminished the ssDNA binding capability of HpSSB, highlighting the crucial role these aromatic residues play in the binding of ssDNA by HpSSB.","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142568681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0