Conversion of arachidonic acid into 14,15,19-trihydroxyeicosa-5,8,11-trienoic acid by Torula dematia NBRC 6213.

IF 2.9 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of bioscience and bioengineering Pub Date : 2025-10-07 DOI:10.1016/j.jbiosc.2025.09.004

Wataru Fujii, Michiki Takeuchi, Si-Bum Park, Kazuki Yagi, Shunta Nakamura, Ei-Tora Yamamura, Jun Ogawa

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引用次数: 0

Abstract

Lipids are one of the three major nutrients that play important roles as sources of energy and as components of cell membranes. Fatty acid metabolites exhibit physiological activities. Among fatty acid metabolites, some hydroxy arachidonic acids (ARAs) have bioactive functions. In this study, we found that Torula dematia NBRC 6213 converts ARA into unknown fatty acids. Liquid chromatography-mass spectrometry (LC-MS), gas chromatography-mass spectrometry, and nuclear magnetic resonance (NMR) analyses were conducted to identify this unknown fatty acid. The unknown fatty acid was identified as 14,15,19-trihydroxyeicosa-5,8,11-trienoic acid. Its production was maximized when T. dematia was cultivated in potato dextrose broth (PDB) medium and reacted for 12 h at pH 7.0 and 28 °C. In addition, multiple intermediates were formed during the conversion of ARA to 14,15,19-trihydroxyeicosa-5,8,11-trienoic acid. LC-MS analysis revealed molecular weights of 320, 336, and 338. This suggests that ARA conversion occurs via the hydroxylation, epoxidation, and hydrolysis of the epoxy group. T. dematia also converts other unsaturated fatty acids into similarly oxidized fatty acids.

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花生四烯酸转化为14,15,19-三羟基二糖-5,8,11-三烯酸的研究。

脂质是三种主要营养素之一，作为能量来源和细胞膜成分起着重要作用。脂肪酸代谢产物具有生理活性。在脂肪酸代谢产物中，一些羟基花生四烯酸具有生物活性。在这项研究中，我们发现Torula dematia NBRC 6213将ARA转化为未知的脂肪酸。采用液相色谱-质谱（LC-MS）、气相色谱-质谱（gc - ms）和核磁共振（NMR）分析对该未知脂肪酸进行鉴定。未知脂肪酸鉴定为14,15,19-三羟基二糖-5,8,11-三烯酸。在马铃薯葡萄糖肉汤（PDB）培养基中培养，在pH 7.0、28℃条件下反应12 h，其产量最大。此外，ARA在转化为14,15,19-三羟基二糖-5,8,11-三烯酸的过程中形成了多个中间体。LC-MS分析显示分子量为320、336和338。这表明ARA转化通过羟基化、环氧化和环氧基水解发生。脱脂腺也将其他不饱和脂肪酸转化为类似的氧化脂肪酸。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of bioscience and bioengineering 生物-生物工程与应用微生物

CiteScore

5.90

自引率

3.60%

发文量

144

审稿时长

51 days

期刊介绍： The Journal of Bioscience and Bioengineering is a research journal publishing original full-length research papers, reviews, and Letters to the Editor. The Journal is devoted to the advancement and dissemination of knowledge concerning fermentation technology, biochemical engineering, food technology and microbiology.