Journal of bioscience and bioengineering最新文献_第2页

Geobacter sulfurreducens strain 60473, a potent bioaugmentation agent for improving the performances of bioelectrochemical systems. Geobacter sulfurreducens 菌株 60473，一种用于提高生物电化学系统性能的强效生物增强剂。

IF 2.3 4区生物学

Journal of bioscience and bioengineering Pub Date : 2025-01-01 Epub Date: 2024-11-06 DOI: 10.1016/j.jbiosc.2024.10.007

Tomoka Harada, Yohei Yamada, Mizuki Toda, Yuki Takamatsu, Keisuke Tomita, Kengo Inoue, Atsushi Kouzuma, Kazuya Watanabe

{"title":"Geobacter sulfurreducens strain 60473, a potent bioaugmentation agent for improving the performances of bioelectrochemical systems.","authors":"Tomoka Harada, Yohei Yamada, Mizuki Toda, Yuki Takamatsu, Keisuke Tomita, Kengo Inoue, Atsushi Kouzuma, Kazuya Watanabe","doi":"10.1016/j.jbiosc.2024.10.007","DOIUrl":"10.1016/j.jbiosc.2024.10.007","url":null,"abstract":"Bioaugmentation with electrochemically active bacteria (EAB) has been suggested useful for improving the performance of bioelectrochemical systems (BESs) for sustainable energy generation, while its success is dependent on EAB introduced into the systems. Here we report on the isolation of a novel EAB, Geobacter sulfurreducens strain 60473, from microbes that colonized on an anode of a sediment microbial fuel cell. This strain is highly adhesive to graphite electrodes, forms dense biofilms on electrode surfaces, and generates high current densities in BESs. When microbial electrolysis cells (MECs) inoculated with paddy-field soil and fed starch as the major organic substrate were augmented with strain 60473, Geobacter bacteria predominantly colonized on anodes, and MEC performances, including current generation, hydrogen production and organics removal, were substantially improved compared to non-bioaugmented controls. Results suggest that bioaugmentation with electrode-adhesive EAB, such as strain 60473, is a promising approach for improving the performance of BESs, including MECs treating fermentable organics and biomass wastes.","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":" ","pages":"36-43"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142604875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolic analysis reveals the contribution of mechanosensitive channel MscM to extracellular release of glutamate in glycogen-deficient Synechococcus elongatus.

IF 2.3 4区生物学

Journal of bioscience and bioengineering Pub Date : 2024-12-20 DOI: 10.1016/j.jbiosc.2024.12.003

Yuichi Kato, Kouhei Kamasaka, Mami Matsuda, Hiroko Koizumi, Ryudo Ohbayashi, Hiroki Ashida, Akihiko Kondo, Tomohisa Hasunuma

{"title":"Metabolic analysis reveals the contribution of mechanosensitive channel MscM to extracellular release of glutamate in glycogen-deficient Synechococcus elongatus.","authors":"Yuichi Kato, Kouhei Kamasaka, Mami Matsuda, Hiroko Koizumi, Ryudo Ohbayashi, Hiroki Ashida, Akihiko Kondo, Tomohisa Hasunuma","doi":"10.1016/j.jbiosc.2024.12.003","DOIUrl":"https://doi.org/10.1016/j.jbiosc.2024.12.003","url":null,"abstract":"In bacteria, mechanosensitive channels mediate extracellular release of osmolytes, including glutamate, functioning as safety valves upon osmotic downshift. In cyanobacteria, the role of mechanosensitive channels has not been completely elucidated. Recently, the glycogen-deficient ΔglgC mutant of Synechococcus elongatus PCC 7942 was found to release glutamate extracellularly, giving rise to a hypothesis that the role of mechanosensitive channels in cyanobacteria is conserved. Using the ΔglgC mutant as the model, the present study aimed to examine whether the putative mechanosensitive channel protein MscM mediates the extracellular release of glutamate. Compared to the ΔglgC mutant, the ΔglgC ΔmscM mutant was found to release less glutamate and aspartate extracellularly. In addition, intracellular levels of these amino acids were significantly higher in the ΔglgC ΔmscM mutant than in the ΔglgC mutant. These results suggested that MscM mediates the extracellular release of glutamate and aspartate in glycogen-deficient cyanobacteria. Furthermore, the ΔglgC ΔmscM mutant exhibited more elongated cell shapes compared to the wild type and ΔglgC single mutant, suggesting that the deletion of the mscM gene intensified turgor pressure and/or that MscM is involved in cell division. Through metabolic analysis, the present study revealed that mechanosensitive channel MscM in cyanobacteria is involved in the extracellular release of amino acids.","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142872154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Specific hydroxylation and glucuronidation of 2'-hydroxyflavanone by Streptomyces coeruleorubidus NRRL B-2569.

IF 2.3 4区生物学

Journal of bioscience and bioengineering Pub Date : 2024-12-19 DOI: 10.1016/j.jbiosc.2024.11.004

Jie Ren, Kyle Jackson, Caleb Don Barton, Yu Huang, Jixun Zhan

{"title":"Specific hydroxylation and glucuronidation of 2'-hydroxyflavanone by Streptomyces coeruleorubidus NRRL B-2569.","authors":"Jie Ren, Kyle Jackson, Caleb Don Barton, Yu Huang, Jixun Zhan","doi":"10.1016/j.jbiosc.2024.11.004","DOIUrl":"https://doi.org/10.1016/j.jbiosc.2024.11.004","url":null,"abstract":"Flavonoids constitute a class of natural compounds with varied bioactivities. Nevertheless, the potential health benefits of flavonoids for humans are often compromised by their low water solubility and limited bioavailability. In this study, four derivatives, namely 2',5'-dihydroxyflavanone (2), 5'-dihydroxyflavone-2'-O-β-d-glucuronide (3), and two isomers of hydroxyflavanone-2'-O-β-d-glucuronide (4 and 5), were biosynthesized from substrate 2'-hydroxyflavanone (1) through the specific hydroxylation and glucuronidation using Streptomyces coeruleorubidus NRRL B-2569. Product 2 was identified as a known compound while products 3-5 were structurally characterized as new structures through extensive 1D and 2D NMR analysis. The water solubility of obtained products 3-5 were enhanced by 36-340 times compared to the substrate. Moreover, the antioxidant assay revealed that product 3 exhibited improved 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity compared to the substrate, decreasing the logIC50 from 10.77 ± 0.05 μM to 9.55 ± 0.05 μM. Compound 3 also displayed significantly higher anticancer activity than the substrate 2'-hydroxyflavanone against Glioblastoma 33 cancer stem cells (GSC33), decreasing the IC50 from 25.05 μM to 7.07 μM. Thus, S. coeruleorubidus NRRL B-2569 stands out as an effective tool for modifying flavonoids, thereby enhancing their water solubility and bioactivities.","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142872239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of in vitro hair pigmentation model using hair follicle organoids. 利用毛囊器官组织开发体外毛发色素沉着模型。

IF 2.3 4区生物学

Journal of bioscience and bioengineering Pub Date : 2024-12-12 DOI: 10.1016/j.jbiosc.2024.11.003

Shan Tu, Tatsuto Kageyama, Jieun Seo, Yinghui Zhou, Junji Fukuda

引用次数: 0

Efficient yeast breeding using a sake metabolome analysis for a strain evaluation.

IF 2.3 4区生物学

Journal of bioscience and bioengineering Pub Date : 2024-12-05 DOI: 10.1016/j.jbiosc.2024.10.010

Risako Kinoshita, Muneyoshi Kanai, Kaoru Takegawa, Kazuhiro Iwashita

{"title":"Efficient yeast breeding using a sake metabolome analysis for a strain evaluation.","authors":"Risako Kinoshita, Muneyoshi Kanai, Kaoru Takegawa, Kazuhiro Iwashita","doi":"10.1016/j.jbiosc.2024.10.010","DOIUrl":"https://doi.org/10.1016/j.jbiosc.2024.10.010","url":null,"abstract":"Breeding sake yeast typically involves generating several gene mutants through UV irradiation or mutagen treatment and selecting those with desired traits based on indicators such as analog resistance. However, this approach often alters traits beyond the target trait due to the random and numerous mutations introduced. To address this issue, we used a previously established metabolome analysis, a sake metabolome analysis, to evaluate the selected yeast strain. After screening for target traits, 110 sake yeast candidates were cultured in yeastnitrogen-based liquid medium using test tubes. The contents were extracted and subjected to comprehensive metabolite analysis through sake metabolome analysis. A phylogenetic tree was then constructed using the metabolome analysis data, enabling the selection of candidate yeasts with only the target traits modified and other traits similar to the parental strain. Selected 21 candidate strains underwent fermentation tests, and the resulting sakes were analyzed using liquid chromatography quadrupole/time-of-flight mass spectrometry (LC-Q/TOF-MS). The findings suggested that the metabolomic data of yeast extracts obtained by simple small-scale culture was similar to the data of resulting sake in the larger-scale fermentation tests. This underscores the utility of metabolome analysis data of yeast extracts in the yeast breeding process, marking the first report proposing the use of the sake metabolome analysis method for yeast breeding.","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142791816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Yeast diversity during the spontaneous fermentation of wine in a winery and in a laboratory using sterilized equipment.

IF 2.3 4区生物学

Journal of bioscience and bioengineering Pub Date : 2024-12-01 DOI: 10.1016/j.jbiosc.2024.11.001

Hideaki Shimizu, Aya Kamada, Takeshi Akao, Yoshiya Kanno, Kazuya Koyama, Kazuhiro Iwashita, Nami Goto-Yamamoto

{"title":"Yeast diversity during the spontaneous fermentation of wine in a winery and in a laboratory using sterilized equipment.","authors":"Hideaki Shimizu, Aya Kamada, Takeshi Akao, Yoshiya Kanno, Kazuya Koyama, Kazuhiro Iwashita, Nami Goto-Yamamoto","doi":"10.1016/j.jbiosc.2024.11.001","DOIUrl":"https://doi.org/10.1016/j.jbiosc.2024.11.001","url":null,"abstract":"A recent trend in some wineries is the return to using spontaneous fermentation, but it is not clear whether winery flora or vineyard microorganisms drive fermentation. We compared fungal communities during the spontaneous fermentation of wine produced in a winery and in a laboratory with sterilized equipment using three grape cultivars (Chardonnay, Merlot, and Muscat Bailey A) obtained from the same harvest. High-throughput sequencing analysis based on the ITS1 region showed that Saccharomyces cerevisiae was the dominant species in winery batches at the end of fermentation, but it was not always dominant in laboratory batches. The number of laboratory batches where S. cerevisiae reached more than 50% at the end of fermentation was only 10 of 26. Consistent with this, in the grape juice/must before fermentation, S. cerevisiae accounted for 1.71% of fungal species identified in winery batches and 0.04% in laboratory batches. In addition, in laboratory-based winemaking, juice clarification of Chardonnay and cold maceration of Merlot influenced the microbial communities observed during fermentation. Our findings suggest that S. cerevisiae present in the winery environment participates at an early stage of fermentation, leading to its dominance at the end in wine produced by spontaneous fermentation in a winery.","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142769289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Topical delivery of gel-in-oil emulsion cocktail with growth factors for the treatment of diabetic pressure ulcers. 用含有生长因子的油凝胶乳液鸡尾酒局部给药治疗糖尿病压疮。

IF 2.3 4区生物学

Journal of bioscience and bioengineering Pub Date : 2024-11-27 DOI: 10.1016/j.jbiosc.2024.10.011

Yi Zhang, Jannatul Fardous, Yu Zhou, Lichun Wu, Ryota Doi, Jia Hu, Yuya Hirota, Yasuhiro Ikegami, Shinichi Aishima, Hiroyuki Ijima

{"title":"Topical delivery of gel-in-oil emulsion cocktail with growth factors for the treatment of diabetic pressure ulcers.","authors":"Yi Zhang, Jannatul Fardous, Yu Zhou, Lichun Wu, Ryota Doi, Jia Hu, Yuya Hirota, Yasuhiro Ikegami, Shinichi Aishima, Hiroyuki Ijima","doi":"10.1016/j.jbiosc.2024.10.011","DOIUrl":"https://doi.org/10.1016/j.jbiosc.2024.10.011","url":null,"abstract":"Healing diabetic foot ulcers (DFUs) poses a serious challenge for many individuals with diabetes. The use of biomaterials applied locally for treating DFUs has recently garnered significant attention. Here, we present a gel-in-oil nanogel dispersion (G/O-NGD) capable of local delivery of six different growth factors (GFs) via the topical route, followed by an in-vivo evaluation in mice. Both macroscopic and microscopic changes in skin structure were evidented after topical application of GF-cocktail G/O-NGD, and changes in CD68 and CD31 levels and collagen content were measured. Expression and synthesis of Interleukin-6 (IL-6), transforming GF beta 1 (TGF-β1), and basic fibroblast GF (bFGF) were also analyzed. The results showed that a significant reduction in ulcer area, restoration of skin structure, increase in collagen content, angiogenesis, and suppression of inflammation were possible with GF-cocktail G/O-NGD, indicating that G/O-NGD is a prospective carrier for local delivery of GF, improving wound healing processes.","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142739786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation methods for decellularized tissues: A focus on human amniotic membrane. 脱细胞组织的评估方法：以人类羊膜为重点。

IF 2.3 4区生物学

Journal of bioscience and bioengineering Pub Date : 2024-11-25 DOI: 10.1016/j.jbiosc.2024.10.009

Miriam Guadalupe Salgado García, Néstor Fabián Díaz, Guadalupe García López, Ikuri Álvarez Maya, Claudia Hernández Jimenez, Yvonne Roman Maldonado, David José Mendoza Aguayo, Néstor Emmanuel Díaz Martínez

引用次数: 0

COP II-mediated ER-to-Golgi transport is a bottleneck for IgNAR-Fc production in the Chinese hamster ovary cell expression system. 在中国仓鼠卵巢细胞表达系统中，COP II 介导的 ER 至高尔基体转运是 IgNAR-Fc 生产的瓶颈。

IF 2.3 4区生物学

Journal of bioscience and bioengineering Pub Date : 2024-11-24 DOI: 10.1016/j.jbiosc.2024.10.012

Xiaofang Lyu, Noriko Yamano-Adachi, Yuichi Koga, Takeshi Omasa

{"title":"COP II-mediated ER-to-Golgi transport is a bottleneck for IgNAR-Fc production in the Chinese hamster ovary cell expression system.","authors":"Xiaofang Lyu, Noriko Yamano-Adachi, Yuichi Koga, Takeshi Omasa","doi":"10.1016/j.jbiosc.2024.10.012","DOIUrl":"https://doi.org/10.1016/j.jbiosc.2024.10.012","url":null,"abstract":"The novel heavy-chain antibody known as immunoglobulin new antigen receptor (IgNAR) is derived from cartilaginous fishes such as sharks. IgNAR, which binds to antigens with the high specificity and affinity of a conventional IgG antibody and exhibits high resistance to denaturation, has potential as a next-generation antibody in biopharmaceutical and biotechnological applications. High-level expression of recombinant IgNAR in animal cells has been challenging. In our previous study, IgNAR was expressed as a fusion protein with a human IgG Fc region (IgNAR-Fc) in Chinese hamster ovary (CHO) cells, but did not meet the production level required for further research and application. In this study, we sought to identify the production bottleneck in CHO cells as a first step toward achieving abundant production of IgNAR. Using an established IgG high-production CHO cell line as a comparator, we found that the amounts of intracellular dimeric IgNAR-Fc produced in CHO cells were similar to those of intracellular dimeric IgG. Furthermore, the majority of intracellular IgNAR-Fc was retained in the endoplasmic reticulum (ER) and strongly colocalized to ERGIC-53, the cargo receptor for coat protein complex II (COP II)-coated vesicles. These findings suggest that COP II-mediated ER-to-Golgi transport may represent a bottleneck for IgNAR-Fc production in the CHO cell expression system.","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142716202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In-situ collagen mineralization modulates metastatic properties of breast cancer cells. 原位胶原矿化可调节乳腺癌细胞的转移特性。

IF 2.3 4区生物学

Journal of bioscience and bioengineering Pub Date : 2024-11-22 DOI: 10.1016/j.jbiosc.2024.07.010

Jaya Thilakan, Sudhir Kumar Goel, Neha Arya

{"title":"In-situ collagen mineralization modulates metastatic properties of breast cancer cells.","authors":"Jaya Thilakan, Sudhir Kumar Goel, Neha Arya","doi":"10.1016/j.jbiosc.2024.07.010","DOIUrl":"https://doi.org/10.1016/j.jbiosc.2024.07.010","url":null,"abstract":"Bone metastasis is the leading cause of morbidity and mortality in advanced-stage breast cancer patients. While most studies focus on the cellular and genetic factors associated with breast cancer metastasis, the role of the extracellular matrix (ECM) of bone in breast cancer metastasis remains elusive. In this study, we recapitulated the bone microenvironment using in-situ mineralized collagen type-I hydrogels and utilized them to understand breast cancer metastasis. Our results indicated successful mineralization of collagen type-I based hydrogels in the presence of serum proteins, which increased as a function of time. There was no difference in the adhesion of breast cancer cells seeded on collagen and mineralized collagen surfaces. However, there was a marked reduction in cell proliferation, down-regulation of various metastatic markers, and decreased migratory phenotype with a concomitant increase in cleaved caspase-3 on mineralized collagen compared to collagen hydrogels. In conclusion, our results suggest an inverse relationship between bone mineralization and the metastatic propensity of breast cancer cells. We further speculate the role of other factors in the skeletal ecosystem for mediating preferential homing of breast cancer cells to the bone microenvironment.","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142695515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0