Journal of bioscience and bioengineering最新文献_第2页

Rapid and practical microbial detection method for brewery hygiene management using the EZ-Fluo system EZ-Fluo系统用于啤酒厂卫生管理的快速实用微生物检测方法。

IF 2.9 4区生物学

Journal of bioscience and bioengineering Pub Date : 2025-09-03 DOI: 10.1016/j.jbiosc.2025.08.002

Maika Kitazawa , Nobuchika Takesue , Kazumaru Iijima , Koji Suzuki , Masaki Shimokawa

{"title":"Rapid and practical microbial detection method for brewery hygiene management using the EZ-Fluo system","authors":"Maika Kitazawa , Nobuchika Takesue , Kazumaru Iijima , Koji Suzuki , Masaki Shimokawa","doi":"10.1016/j.jbiosc.2025.08.002","DOIUrl":"10.1016/j.jbiosc.2025.08.002","url":null,"abstract":"<div><div>The early detection of microorganisms in the brewery environment is crucial for taking quick corrective actions and minimizing the risk of microbial contamination. However, hygiene tests using traditional culture methods for visual detection have poor turnaround times. In this study, we optimized the test conditions for the EZ-Fluo Rapid Detection System for brewery hygiene management. In this system, microorganisms are trapped on a membrane filter, cultured on agar, and detected after brief incubation using fluorescence-based technology with a dedicated detector. Among several alternatives, the EZ-Fluo system was chosen for its cost-effectiveness. We determined the appropriate test conditions, including the incubation temperature, fluorescent reagent, and temperature for the fluorescence reaction, using two slow growing environmental indicator species, <em>Acetobacter pasteurianus</em> and <em>Pseudomonas fluorescens</em>. Using the EZ-Fluo system under our optimized detection conditions (hereafter referred to as the EZF-ODC method), nine indicator species common to breweries were detected at a recovery rate of 54.0–117.2 % after only 30 h of incubation. No significant difference was found in colony-forming units among the analysts (<em>p</em> > 0.05). In brewery hygiene tests, the EZF-ODC method (30-h incubation) and the traditional culture method (72-h incubation) yielded comparable detection profiles, with positive or negative results matching in 94.6 % of the 222 samples. The EZF-ODC method can provide results by the following day, representing a significant improvement over the 3 days needed for traditional methods and enabling the rapid implementation of hygiene control measures to maintain a clean brewery environment and possibly expedite production planning.</div></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"140 5","pages":"Pages 314-319"},"PeriodicalIF":2.9,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145000633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Locus heterogeneity in the mechanism underlying the superior fermentation performances of phylogenetically distant sake yeasts 系统发育较远的清酒酵母优越发酵性能的基因座异质性机制。

IF 2.9 4区生物学

Journal of bioscience and bioengineering Pub Date : 2025-09-02 DOI: 10.1016/j.jbiosc.2025.08.005

Kotaro Mori , Taisuke Seike , Takeshi Akao , Yoshifumi Takao , Tasuku Yamada , Toshinari Takahashi , Fumio Matsuda

{"title":"Locus heterogeneity in the mechanism underlying the superior fermentation performances of phylogenetically distant sake yeasts","authors":"Kotaro Mori , Taisuke Seike , Takeshi Akao , Yoshifumi Takao , Tasuku Yamada , Toshinari Takahashi , Fumio Matsuda","doi":"10.1016/j.jbiosc.2025.08.005","DOIUrl":"10.1016/j.jbiosc.2025.08.005","url":null,"abstract":"<div><div>Sake yeasts, <em>Saccharomyces cerevisiae</em> strains isolated from Japanese sake fermentation tanks, exhibit superior fermentation performance to that of other yeast strains. Although the exceptional abilities of the modern sake yeast strain K701 have been extensively investigated, those of phylogenetically distant classical sake yeasts remain largely understudied. In this study, we aimed to clarify the mechanism underlying the superior fermentation ability of the classical sake yeast strain Km67 by comparing its genetic and physiological properties with those of the laboratory strains X2180 and K701. Km67 did not exhibit quiescence-specific phenotypes in sake mashes in the same manner as K701. RNA sequencing revealed similar trends between the transcriptomes of Km67 and K701 in sake mash, suggesting that the lack of quiescence entry was related to the higher fermentation ability of Km67. Genetic testing demonstrated that signals upstream of Rim15p were not conveyed downstream, indicating impaired fermentation repression mediated by Rim15p in Km67 cells. Protein phosphatase 2A (PP2A<sup>B55δ</sup>) activity declined following <em>CDC55</em> deletion downstream of Rim15p, consequently reducing the fermentation performance of Km67. However, the extent of fermentation reduction upon <em>CDC55</em> deletion was not as large as that with X2180 and K701. This suggested the presence of unidentified factors that regulated fermentation independently of PP2A<sup>B55δ</sup> or Rim15p in Km67. Thus, our findings demonstrate locus heterogeneity in the mechanism underlying fermentation abilities between the phylogenetically distant sake yeasts Km67 and K701.</div></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"140 5","pages":"Pages 306-313"},"PeriodicalIF":2.9,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144955388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural determinants of the thermostability of d-amino acid oxidase of the thermophilic fungus Rasamsonia emersonii. 嗜热真菌桑椹d-氨基酸氧化酶热稳定性的结构决定因素。

IF 2.9 4区生物学

Journal of bioscience and bioengineering Pub Date : 2025-09-01 Epub Date: 2025-06-27 DOI: 10.1016/j.jbiosc.2025.06.003

Takehiro Furuichi, Yuya Shimekake, Daiki Imanishi, Shouji Takahashi

{"title":"Structural determinants of the thermostability of d-amino acid oxidase of the thermophilic fungus Rasamsonia emersonii.","authors":"Takehiro Furuichi, Yuya Shimekake, Daiki Imanishi, Shouji Takahashi","doi":"10.1016/j.jbiosc.2025.06.003","DOIUrl":"10.1016/j.jbiosc.2025.06.003","url":null,"abstract":"<p><p>d-Amino acid oxidase from the thermophilic fungus Rasamsonia emersonii strain YA (ReDAAO) exhibits high thermostability. To understand the structural basis for this high stability, we isolated thermolabile variants of ReDAAO with a single amino acid substitution (L134P, K203E, C230S, V275G, and V305L), whose T<sub>50</sub> (the temperature at which 50 % of the initial enzyme activity was retained) values were 12-18 °C lower than that of the wild-type. The L134P substitution in a flexible protein surface loop caused the most severe destabilization, likely due to increased loop flexibility through hydrogen bond disruption. The other substitutions affected stability by impairing distinct structural elements: K203E might disrupt an amino acid interaction network involved in both flavin adenine dinucleotide binding and subunit interactions, C230S might eliminate the unique disulfide bond that likely fixes a long α-helix involved in subunit interactions, and V275G and V305L might perturb critical interactions at subunit interfaces, with V305L also potentially affecting the subunit structure. Notably, the thermostabilization conferred by the disulfide bond and the interaction network involving K203 were unique to thermophilic fungal DAAOs. These findings revealed multiple distinct mechanisms of thermostabilization in ReDAAO, providing valuable insights for engineering flavoenzymes with improved thermostability.</p>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":" ","pages":"132-139"},"PeriodicalIF":2.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficient selection of pyruvate decarboxylase sequences from database for high ethanol productivity in Synechocystis sp. PCC 6803. 聚囊藻PCC 6803丙酮酸脱羧酶序列的高效筛选。

IF 2.9 4区生物学

Journal of bioscience and bioengineering Pub Date : 2025-09-01 Epub Date: 2025-06-19 DOI: 10.1016/j.jbiosc.2025.05.011

Hiroki Nishiguchi, Teppei Niide, Yoshihiro Toya, Hiroshi Shimizu

{"title":"Efficient selection of pyruvate decarboxylase sequences from database for high ethanol productivity in Synechocystis sp. PCC 6803.","authors":"Hiroki Nishiguchi, Teppei Niide, Yoshihiro Toya, Hiroshi Shimizu","doi":"10.1016/j.jbiosc.2025.05.011","DOIUrl":"10.1016/j.jbiosc.2025.05.011","url":null,"abstract":"<p><p>Ethanol production using the model cyanobacterium Synechocystis sp. PCC 6803 (PCC6803) has garnered considerable attention. A heterologous pyruvate decarboxylase (PDC) is essential for synthesizing ethanol in PCC6803. Although many organisms possess PDCs, no systematic search for suitable PDCs has been reported. This study employed a two-step approach to identify promising PDCs. First, nine diverse natural PDCs with confirmed activity in BRENDA were evaluated for ethanol production in PCC6803. Ethanol production was observed only with PDCs from Zymomonas mobilis (Zm PDC) and Gluconobacter diazotrophicus, suggesting that bacterial PDCs are suitable. In the second step, the search focused on bacterial PDCs, not only natural PDCs but also artificial sequences designed via the Protein Repair One-Stop Shop or ancestral sequence reconstruction. A PDC from Gluconobacter oxydans showed higher ethanol productivity (88.9 mg/L/5 days) than Zm PDC. Although productivity did not surpass that of Zm PDC, ethanol production was achieved with previously unconfirmed or engineered PDCs, expanding the range of useable sequences. This stepwise strategy demonstrates a robust approach for identifying and designing useful enzymes across sequence spaces.</p>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":" ","pages":"123-131"},"PeriodicalIF":2.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144336533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid and sensitive detection of alkaline phosphatase based on fluorescent gold nanoclusters and p-nitrophenyl phosphate. 基于荧光金纳米团簇和对硝基苯磷酸的碱性磷酸酶快速灵敏检测。

IF 2.9 4区生物学

Journal of bioscience and bioengineering Pub Date : 2025-09-01 Epub Date: 2025-07-04 DOI: 10.1016/j.jbiosc.2025.06.004

So-Hee Kim, Chang Soon Huh, Moon-Moo Kim

{"title":"Rapid and sensitive detection of alkaline phosphatase based on fluorescent gold nanoclusters and p-nitrophenyl phosphate.","authors":"So-Hee Kim, Chang Soon Huh, Moon-Moo Kim","doi":"10.1016/j.jbiosc.2025.06.004","DOIUrl":"10.1016/j.jbiosc.2025.06.004","url":null,"abstract":"<p><p>Alkaline phosphatase (ALP) is an essential enzyme that is involved in various metabolic processes. Abnormal ALP levels are linked to diseases and pathological conditions. Herein, a simple and sensitive assay is reported for ALP detection by using glutathione-conjugated gold nanoclusters (GSH-AuNCs) and p-nitrophenyl phosphate (pNPP), based on the fluorescence quenching mechanism. In the underlying mechanism of this assay, the fluorescence of GSH-AuNCs is initially quenched by pNPP, followed by further quenching caused by p-nitrophenol (pNP), a product of ALP activity. To investigate this mechanism for the diagnostic ALP detection, UV-Vis spectrophotometry, transmission electron microscopy (TEM), and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis were employed, and this method was tested in real samples. The prepared GSH-AuNCs exhibited an absorption peak at 600 nm under excitation at 365 nm. TEM analysis revealed that GSH-AuNCs were spherical in shape and exhibited uniform particle size and distribution. Furthermore, the gradual reduction in fluorescence intensity of GSH-AuNCs was observed with increasing concentration of pNPP increased (0.03 mM-2.7 mM), suggesting the quenching of the fluorescence by pNPP. SDS-PAGE analysis further confirmed the quenching effect of pNPP on GSH-AuNCs. In addition, fluorescence intensity was decreased by the increasing amounts of ALP. The relationship curve revealed a detectable concentration range of 1.95-1000 U/L and the correlation coefficient of 0.976. The developed method was successfully applied to human osteosarcoma MG-63 cell lysates, culture medium, and extracts from root plants for detection of ALP. Therefore, this assay will be beneficial for the diagnosis of ALP activity in clinical medicine.</p>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":" ","pages":"162-167"},"PeriodicalIF":2.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144567474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nutrient factors specifically promoting ethanol fermentation but not growth in yeast. 营养因子特别促进乙醇发酵，但不促进酵母生长。

IF 2.9 4区生物学

Journal of bioscience and bioengineering Pub Date : 2025-09-01 Epub Date: 2025-07-01 DOI: 10.1016/j.jbiosc.2025.06.007

Satoshi Ebe, Yukie Misumi, Yuki Terauchi, Naruyuki Maruoka, Hideharu Takashita, Hisashi Hoshida, Rinji Akada

{"title":"Nutrient factors specifically promoting ethanol fermentation but not growth in yeast.","authors":"Satoshi Ebe, Yukie Misumi, Yuki Terauchi, Naruyuki Maruoka, Hideharu Takashita, Hisashi Hoshida, Rinji Akada","doi":"10.1016/j.jbiosc.2025.06.007","DOIUrl":"10.1016/j.jbiosc.2025.06.007","url":null,"abstract":"<p><p>Yeast ethanol fermentation and growth are affected by environmental factors such as nutrients, pH, and temperature. Ethanol fermentation in yeast is typically accompanied by cell proliferation. Thus, the specific nutrients required for fermentation remain unclear. To determine nutrients required for fermentation, first a semi-synthetic medium with a small inoculum size (OD<sub>600</sub> = 2) was used, and CO<sub>2</sub> gas emissions were monitored. The addition of sodium aspartate (Asp) and MgSO<sub>4</sub> to a medium containing 0.2 % yeast extract and 9.1 % glucose efficiently increased gas emissions. Further addition of KH<sub>2</sub>PO<sub>4</sub> and myo-inositol to the medium increased the fermentation rate. Next, to obtain no-growth conditions during fermentation, the glucose and cell amounts were increased. Cell growth was repressed but fermentation proceeded in 28.6 % glucose with a large inoculum size (OD<sub>600</sub> = 16 or 32). The elimination of medium components under these conditions revealed that Asp was sufficient to increase gas emissions without cell growth. Further analysis indicated that pH maintenance and aspartic acid are required to enhance fermentation under no-growth conditions.</p>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":" ","pages":"146-153"},"PeriodicalIF":2.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144553676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioelectrochemical reduction of pyruvate to d-lactate by engineered Shewanella oneidensis MR-1. 工程希瓦氏菌MR-1生物电化学还原丙酮酸为d-乳酸。

IF 2.9 4区生物学

Journal of bioscience and bioengineering Pub Date : 2025-09-01 Epub Date: 2025-07-05 DOI: 10.1016/j.jbiosc.2025.06.001

Soshi Taguchi, Atsumi Hirose, Atsushi Kouzuma, Kazuya Watanabe

{"title":"Bioelectrochemical reduction of pyruvate to d-lactate by engineered Shewanella oneidensis MR-1.","authors":"Soshi Taguchi, Atsumi Hirose, Atsushi Kouzuma, Kazuya Watanabe","doi":"10.1016/j.jbiosc.2025.06.001","DOIUrl":"10.1016/j.jbiosc.2025.06.001","url":null,"abstract":"<p><p>Shewanella oneidensis MR-1 possesses an extracellular electron transfer (EET) pathway that enables bidirectional electron exchange with electrodes, making it a promising host for electro-fermentation (EF). However, the intracellular redox reactions driven by MR-1 during electron uptake from the electrodes remain poorly characterized. This study investigated the metabolic fate of pyruvate, a key fermentation intermediate, during inward electron transfer from a low-potential cathode. To examine this, an MR-1 derivative lacking formate dehydrogenase (ΔFDH), which is unable to utilize formate as an electron donor for pyruvate reduction, was incubated under open-circuit (OC) conditions and closed-circuit (CC) conditions with an electrode poised at -0.36 V (vs. the standard hydrogen electrode). A comparative analysis of pyruvate-derived metabolites under these conditions revealed that ΔFDH produced significantly higher amounts of d-lactate under CC conditions, indicating cathode-derived electron utilization for pyruvate reduction to d-lactate. Further gene knockout experiments in the ΔFDH background showed that two d-lactate dehydrogenases (D-LDHs) in MR-1, Dld (a quinone-dependent inner membrane D-LDH) and LdhA (an NADH-dependent D-LDH), contributed almost equally to cathode-dependent d-lactate production. These results indicate that electron transfer from electrodes to pyruvate in MR-1 cells involves both inner membrane quinone-mediated and NADH-mediated redox reactions, highlighting the potential applicability of MR-1 in diverse EF processes.</p>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":" ","pages":"140-145"},"PeriodicalIF":2.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144575548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In silico design of smaller size enzymatic protein by generative artificial intelligence (ProtGPT2). 基于生成式人工智能（ProtGPT2）的小尺寸酶蛋白的计算机设计。

IF 2.9 4区生物学

Journal of bioscience and bioengineering Pub Date : 2025-09-01 Epub Date: 2025-07-10 DOI: 10.1016/j.jbiosc.2025.06.009

Hiroyuki Hamada, Tamon Matsuzawa, Taizo Hanai

{"title":"In silico design of smaller size enzymatic protein by generative artificial intelligence (ProtGPT2).","authors":"Hiroyuki Hamada, Tamon Matsuzawa, Taizo Hanai","doi":"10.1016/j.jbiosc.2025.06.009","DOIUrl":"10.1016/j.jbiosc.2025.06.009","url":null,"abstract":"<p><p>The construction of small proteins by removing amino acid subsequences that are not involved in function, activity, or structure is crucial for bioprocessing and drug development. Traditional design methods often focus on reconstructing functional motifs, but they face challenges in stabilizing structure and reproducing function. In this study, we aimed to develop a design method for small proteins using ProtGPT2, a model that generates protein sequences based on function and structure. First, amino acid sequence data of malate dehydrogenase (MDH) was collected, and ProtGPT2 was fine-tuned (ProtGPT2 for MDH). The chain length and perplexity (ppl) of the generated sequences were evaluated, producing shorter sequences than the natural ones. The validity of the generated sequences was assessed using both population and individual analyses. Population analysis, including multiple sequence alignment (MSA) and t-distributed stochastic neighbor embedding (tSNE), revealed that ProtGPT2 for MDH identified functional motifs of MDH and incorporated them into the generated sequences. Additionally, tSNE showed that the generated sequences were highly similar to natural MDH sequences. In individual analysis, 10 randomly selected sequences were evaluated using BLAST, AlphaFold2, and InterPro. BLAST indicated that 9 sequences were novel MDH variants. AlphaFold2 confirmed that their 3D structures were highly similar to known MDH structures. InterPro identified domains and active sites in 2 sequences, suggesting that they were novel, small MDH variants. In conclusion, ProtGPT2 for MDH has the potential to design amino acid sequence candidates for small MDHs. The validity and utility of the model will be established through future experimental efforts.</p>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":" ","pages":"174-179"},"PeriodicalIF":2.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144618116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of vascular endothelial cells on the neurite outgrowth of nerve cells. 血管内皮细胞对神经细胞突突生长的影响。

IF 2.9 4区生物学

Journal of bioscience and bioengineering Pub Date : 2025-09-01 Epub Date: 2025-06-16 DOI: 10.1016/j.jbiosc.2025.05.010

Tomoki Asaba, Tatsuya Osaki, Koki Murayama, Sayuri Hamano, Tatsuto Kageyama, Junji Fukuda

引用次数: 0

Enhanced antibody production in Chinese hamster ovary cell cultures supplemented with barley shochu distillation by-product supernatant 添加大麦烧酒蒸馏副产物上清液增强中国仓鼠卵巢细胞抗体产生。

IF 2.9 4区生物学

Journal of bioscience and bioengineering Pub Date : 2025-09-01 DOI: 10.1016/j.jbiosc.2025.08.004

Akihiro Nakamura , Masafumi Kadowaki , Kazuya Tsujioka , Takashi Kaieda , Masamichi Kamihira

{"title":"Enhanced antibody production in Chinese hamster ovary cell cultures supplemented with barley shochu distillation by-product supernatant","authors":"Akihiro Nakamura , Masafumi Kadowaki , Kazuya Tsujioka , Takashi Kaieda , Masamichi Kamihira","doi":"10.1016/j.jbiosc.2025.08.004","DOIUrl":"10.1016/j.jbiosc.2025.08.004","url":null,"abstract":"<div><div>Antibody production in Chinese hamster ovary (CHO) cell culture was enhanced by supplementing the culture medium with barley shochu distillation by-product supernatant (BX2). To predict antibody production following BX2 addition, fed-batch culture experiments were conducted under varying BX2 conditions using a response surface methodology. BX2 supplementation was predicted to improve antibody production by 138 %, 146 %, 120 %, and 240 % in IgG-producing CHO-MK1, CHO-MK2, CHO-DG44, and Fc-fusion protein-producing CHO-DG44 cells, respectively, compared to controls without BX2. BX2 consisted primarily of 27 % (w/w) bound amino acids, 19 % (w/w) free amino acids, 15 % (w/w) organic acids, 14 % (w/w) glycerol, 14 % (w/w) bound sugars, 6 % (w/w) ash, and 5 % (w/w) free sugars within the soluble solids. BX2 was roughly fractionated into free components (e.g., sugars, amino acids, organic acids, and glycerol fraction) and bound components (e.g., polysaccharides, peptides, peptide-linked sugars, and glycosylated amino acids fraction) using reverse-phase high-performance liquid chromatography. In cultures of IgG-producing CHO-MK2 cells, the addition of BX2 or its bound components enhanced IgG production by 170 % and 117 %, respectively. In contrast, the free components fraction reduced IgG production to 73 % of that observed without BX2. These results suggest that BX2 enhances antibody production through the combined action of multiple components rather than a single component. This study demonstrates that BX2 is an effective additive for enhancing antibody production in CHO cells cultured in high-performance medium typically used in industrial applications.</div></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"140 5","pages":"Pages 341-349"},"PeriodicalIF":2.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144955296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0