Journal of bioscience and bioengineering最新文献_第2页

Development of in vitro hair pigmentation model using hair follicle organoids 利用毛囊器官组织开发体外毛发色素沉着模型。

IF 2.3 4区生物学

Journal of bioscience and bioengineering Pub Date : 2025-02-01 DOI: 10.1016/j.jbiosc.2024.11.003

Shan Tu , Tatsuto Kageyama , Jieun Seo , Yinghui Zhou , Junji Fukuda

{"title":"Development of in vitro hair pigmentation model using hair follicle organoids","authors":"Shan Tu , Tatsuto Kageyama , Jieun Seo , Yinghui Zhou , Junji Fukuda","doi":"10.1016/j.jbiosc.2024.11.003","DOIUrl":"10.1016/j.jbiosc.2024.11.003","url":null,"abstract":"<div><div>Hair color is formed through a series of processes such as melanin synthesis and storage in melanosomes, transfer from melanocytes, and reception by hair matrix cells in the hair bulb. Because gray hair is caused by the deterioration of a single or multiple of these processes, understanding the mechanisms responsible for these processes is crucial for developing therapeutic strategies. Recently, a robust approach for preparing hair follicle organoids (HFOs) was reported, in which hair follicle morphogenesis, including hair shaft elongation, was tracked <em>in vitro</em>. Here, we investigated whether HFOs could be used to assess genes involved in hair pigmentation. HFOs generated hair follicles and pigmented shafts during the <em>in vitro</em> culturing process. The knockdown of genes associated with melanosome production (<em>Bcl2</em> and <em>Mitf</em>) and transport (<em>MyoX</em>, <em>PAR2</em>, and <em>Rab11b</em>) significantly increased the number of gray hairs in HFOs. This organoid model may be a promising platform for better understanding hair pigmentation and screening drugs for gray hair.</div></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"139 2","pages":"Pages 141-146"},"PeriodicalIF":2.3,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142822209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of a new peak detection function for selecting a phase-appropriate multi-attribute method system 用于选择相位合适的多属性方法系统的新峰值检测函数的比较。

IF 2.3 4区生物学

Journal of bioscience and bioengineering Pub Date : 2025-02-01 DOI: 10.1016/j.jbiosc.2024.10.005

Eriko Numao, Kumi Yanagisawa, Yuki Yagi, Daisuke Tsuchida, Katsuyoshi Yamazaki

{"title":"Comparison of a new peak detection function for selecting a phase-appropriate multi-attribute method system","authors":"Eriko Numao, Kumi Yanagisawa, Yuki Yagi, Daisuke Tsuchida, Katsuyoshi Yamazaki","doi":"10.1016/j.jbiosc.2024.10.005","DOIUrl":"10.1016/j.jbiosc.2024.10.005","url":null,"abstract":"<div><div>The multi-attribute method (MAM) has been recognized as an optimal tool for quality control in biotherapeutics. New peak detection (NPD) is one of the functions of MAM for detecting unexpected differences in samples and is an essential feature required for replacing conventional methods with MAM. Not only used for release and stability testing, NPD is also considered valuable for evaluating comparability and identifying product quality attributes in the research phase. Although many researchers consider the processing parameter the key to NPD, the details of the decision-making process are unclear. Besides specific instruments and software packages has been used almost exclusively, yet the differences in NPD function between other choices have not been confirmed. Thus, this research aimed to confirm the applicability of our original decision-making approach for NPD processing parameters using two different systems. After optimization for each, under a condition that detected crucial differences and did not return false positives, they differed in the reproducibility of the results. To our knowledge, this was the first time the comparison of NPD results of different systems has been published, and the eligibility of processing methods was evaluated in light of the equivalency of conventional methods' detectability. The findings suggested that the capability of NPD is determined not only by the instrument's resolution but also by the software's capability. Our approach for optimizing the NPD processing parameter is deemed widely applicable and practical in developing therapeutic proteins. The revealed difference will help us select the fit-for-purpose system.</div></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"139 2","pages":"Pages 156-163"},"PeriodicalIF":2.3,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142621135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In-situ collagen mineralization modulates metastatic properties of breast cancer cells 原位胶原矿化可调节乳腺癌细胞的转移特性。

IF 2.3 4区生物学

Journal of bioscience and bioengineering Pub Date : 2025-02-01 DOI: 10.1016/j.jbiosc.2024.07.010

Jaya Thilakan , Sudhir Kumar Goel , Neha Arya

{"title":"In-situ collagen mineralization modulates metastatic properties of breast cancer cells","authors":"Jaya Thilakan , Sudhir Kumar Goel , Neha Arya","doi":"10.1016/j.jbiosc.2024.07.010","DOIUrl":"10.1016/j.jbiosc.2024.07.010","url":null,"abstract":"<div><div>Bone metastasis is the leading cause of morbidity and mortality in advanced-stage breast cancer patients. While most studies focus on the cellular and genetic factors associated with breast cancer metastasis, the role of the extracellular matrix (ECM) of bone in breast cancer metastasis remains elusive. In this study, we recapitulated the bone microenvironment using <em>in-situ</em> mineralized collagen type-I hydrogels and utilized them to understand breast cancer metastasis. Our results indicated successful mineralization of collagen type-I based hydrogels in the presence of serum proteins, which increased as a function of time. There was no difference in the adhesion of breast cancer cells seeded on collagen and mineralized collagen surfaces. However, there was a marked reduction in cell proliferation, down-regulation of various metastatic markers, and decreased migratory phenotype with a concomitant increase in cleaved caspase-3 on mineralized collagen compared to collagen hydrogels. In conclusion, our results suggest an inverse relationship between bone mineralization and the metastatic propensity of breast cancer cells. We further speculate the role of other factors in the skeletal ecosystem for mediating preferential homing of breast cancer cells to the bone microenvironment.</div></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"139 2","pages":"Pages 123-132"},"PeriodicalIF":2.3,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142695515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficient yeast breeding using a sake metabolome analysis for a strain evaluation 利用清酒代谢组分析进行菌种评价的高效酵母育种。

IF 2.3 4区生物学

Journal of bioscience and bioengineering Pub Date : 2025-02-01 DOI: 10.1016/j.jbiosc.2024.10.010

Risako Kinoshita , Muneyoshi Kanai , Kaoru Takegawa , Kazuhiro Iwashita

{"title":"Efficient yeast breeding using a sake metabolome analysis for a strain evaluation","authors":"Risako Kinoshita , Muneyoshi Kanai , Kaoru Takegawa , Kazuhiro Iwashita","doi":"10.1016/j.jbiosc.2024.10.010","DOIUrl":"10.1016/j.jbiosc.2024.10.010","url":null,"abstract":"<div><div>Breeding sake yeast typically involves generating several gene mutants through UV irradiation or mutagen treatment and selecting those with desired traits based on indicators such as analog resistance. However, this approach often alters traits beyond the target trait due to the random and numerous mutations introduced. To address this issue, we used a previously established metabolome analysis, a sake metabolome analysis, to evaluate the selected yeast strain. After screening for target traits, 110 sake yeast candidates were cultured in yeastnitrogen-based liquid medium using test tubes. The contents were extracted and subjected to comprehensive metabolite analysis through sake metabolome analysis. A phylogenetic tree was then constructed using the metabolome analysis data, enabling the selection of candidate yeasts with only the target traits modified and other traits similar to the parental strain. Selected 21 candidate strains underwent fermentation tests, and the resulting sakes were analyzed using liquid chromatography quadrupole/time-of-flight mass spectrometry (LC-Q/TOF-MS). The findings suggested that the metabolomic data of yeast extracts obtained by simple small-scale culture was similar to the data of resulting sake in the larger-scale fermentation tests. This underscores the utility of metabolome analysis data of yeast extracts in the yeast breeding process, marking the first report proposing the use of the sake metabolome analysis method for yeast breeding.</div></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"139 2","pages":"Pages 100-105"},"PeriodicalIF":2.3,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142791816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Topical delivery of gel-in-oil emulsion cocktail with growth factors for the treatment of diabetic pressure ulcers 用含有生长因子的油凝胶乳液鸡尾酒局部给药治疗糖尿病压疮。

IF 2.3 4区生物学

Journal of bioscience and bioengineering Pub Date : 2025-02-01 DOI: 10.1016/j.jbiosc.2024.10.011

Yi Zhang , Jannatul Fardous , Yu Zhou , Lichun Wu , Ryota Doi , Jia Hu , Yuya Hirota , Yasuhiro Ikegami , Shinichi Aishima , Hiroyuki Ijima

{"title":"Topical delivery of gel-in-oil emulsion cocktail with growth factors for the treatment of diabetic pressure ulcers","authors":"Yi Zhang , Jannatul Fardous , Yu Zhou , Lichun Wu , Ryota Doi , Jia Hu , Yuya Hirota , Yasuhiro Ikegami , Shinichi Aishima , Hiroyuki Ijima","doi":"10.1016/j.jbiosc.2024.10.011","DOIUrl":"10.1016/j.jbiosc.2024.10.011","url":null,"abstract":"<div><div>Healing diabetic foot ulcers (DFUs) poses a serious challenge for many individuals with diabetes. The use of biomaterials applied locally for treating DFUs has recently garnered significant attention. Here, we present a gel-in-oil nanogel dispersion (G/O-NGD) capable of local delivery of six different growth factors (GFs) via the topical route, followed by an in-vivo evaluation in mice. Both macroscopic and microscopic changes in skin structure were evidented after topical application of GF-cocktail G/O-NGD, and changes in CD68 and CD31 levels and collagen content were measured. Expression and synthesis of Interleukin-6 (IL-6), transforming GF beta 1 (TGF-β1), and basic fibroblast GF (bFGF) were also analyzed. The results showed that a significant reduction in ulcer area, restoration of skin structure, increase in collagen content, angiogenesis, and suppression of inflammation were possible with GF-cocktail G/O-NGD, indicating that G/O-NGD is a prospective carrier for local delivery of GF, improving wound healing processes.</div></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"139 2","pages":"Pages 112-122"},"PeriodicalIF":2.3,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142739786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protective role of hematin in alcohol oxidase-mediated glycerol oxidation: A kinetic modeling approach.

IF 2.3 4区生物学

Journal of bioscience and bioengineering Pub Date : 2025-01-30 DOI: 10.1016/j.jbiosc.2024.12.012

Adrián Parodi, Agostina Córdoba, Mariano Asteasuain, Ivana Magario

{"title":"Protective role of hematin in alcohol oxidase-mediated glycerol oxidation: A kinetic modeling approach.","authors":"Adrián Parodi, Agostina Córdoba, Mariano Asteasuain, Ivana Magario","doi":"10.1016/j.jbiosc.2024.12.012","DOIUrl":"https://doi.org/10.1016/j.jbiosc.2024.12.012","url":null,"abstract":"<p><p>Mathematical modeling of complex multi-catalytic reaction pathways is a powerful tool not only for determining optimal operational conditions but also for gaining insights into the kinetic behavior of efficient yet product-sensitive enzymes. This approach was applied to the biooxidation of glycerol using oxidases, aided by hematin, a natural mimic of heme peroxidases and catalases, which acts as a scavenger for enzymatically produced H<sub>2</sub>O<sub>2</sub>. The bi-catalytic reaction system was coupled with the formation of a colored imine product from phenol and 4-aminoantipyrine, enabling spectrophotometric monitoring of reaction kinetics. Alcohol oxidase (AOX) from Komagataella pastoris (formerly Pichia pastoris) was submitted to this strategy to overcome some kinetic drawbacks previously observed with galactose oxidase (GAO) from Dactylium dendroides. Although both enzymes exhibited comparable oxidation yields, computer-aided kinetic analyses revealed significant differences in efficiency and sensitivity to H<sub>2</sub>O<sub>2</sub>. AOX demonstrated two orders of magnitude greater efficiency and a similarly higher affinity for glycerol. However, AOX showed a stronger tendency to bind H<sub>2</sub>O<sub>2</sub> compared to hematin or GAO, as suggested by the ratio of the inhibition constant (k<sub>inh</sub>) to the activation constant (k<sub>1</sub>) for hematin. The kinetic model was also used to simulate optimal conditions for enhancing the stability of AOX and hematin. Reducing glycerol concentration or increasing hematin concentration improved AOX stability, while the addition of phenol helped preserve hematin. The predicted stabilities turn the AOX/hematin/phenol system promising for biosensing or analytical applications.</p>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143074513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gas chromatography-mass spectrometry metabolic profiling and sensory evaluation of greenhouse mangoes (Mangifera indica L. 'Irwin') over multiple harvest seasons.

IF 2.3 4区生物学

Journal of bioscience and bioengineering Pub Date : 2025-01-27 DOI: 10.1016/j.jbiosc.2024.12.013

Miwa Sato, Eiichiro Fukusaki

{"title":"Gas chromatography-mass spectrometry metabolic profiling and sensory evaluation of greenhouse mangoes (Mangifera indica L. 'Irwin') over multiple harvest seasons.","authors":"Miwa Sato, Eiichiro Fukusaki","doi":"10.1016/j.jbiosc.2024.12.013","DOIUrl":"https://doi.org/10.1016/j.jbiosc.2024.12.013","url":null,"abstract":"<p><p>Compared to outdoor mango cultivation in the tropics, greenhouse cultivation in temperate regions is less reported due to its short history and small scale. Here, we evaluated for the first time the taste-focused quality of greenhouse-grown mangoes (Irwin) by GC-MS metabolic profiling and sensory evaluation for over three years (2021-2023). The relative standard deviation in sensory evaluation scores was approximately 15 % each year. Meanwhile, principal component analysis was performed on 45 identified metabolites and clustered with similar topology over three years. Orthogonal partial least squares regression analysis showed that a prediction model could be constructed with R<sub>2</sub>, Q<sub>2</sub> = 0.9 or higher for all three years. Furthermore, the 2021 prediction model was used to evaluate the accuracy of sensory evaluation scores in a different year (2023). Compared to the model that used all 45 metabolites as explanatory variables, the accuracy of the model improved when using only 24 important metabolites which are common to both years (2021 and 2022), suggesting that these metabolites are highly reproducible across years. This study would contribute not only to fundamental greenhouse information, but also to the improvement of quality and cultivation methods in greenhouse in the future.</p>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143059100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ROS-responsive nanocarrier for oral delivery of monascin and enhanced alleviation of oxidative stress. ROS 响应型纳米载体，用于口服输送单链蛋白并增强对氧化应激的缓解作用。

IF 2.3 4区生物学

Journal of bioscience and bioengineering Pub Date : 2025-01-25 DOI: 10.1016/j.jbiosc.2025.01.003

Xueyi Chen, Wenli Dai, Wanqi Liang, Dong Li, Wo-Qi Cai, Chan Zhang, Wenlin Hu, Bo-Bo Zhang, Qiongqiong Yang

{"title":"ROS-responsive nanocarrier for oral delivery of monascin and enhanced alleviation of oxidative stress.","authors":"Xueyi Chen, Wenli Dai, Wanqi Liang, Dong Li, Wo-Qi Cai, Chan Zhang, Wenlin Hu, Bo-Bo Zhang, Qiongqiong Yang","doi":"10.1016/j.jbiosc.2025.01.003","DOIUrl":"https://doi.org/10.1016/j.jbiosc.2025.01.003","url":null,"abstract":"<p><p>Oxidative stress, caused by excessive production of reactive oxygen species (ROS), plays a crucial role in the occurrence and development of various diseases. Monascin can scavenge ROS and alleviate oxidative stress but with a low fermentation rate and bioavailability. Here, we optimized the fermentation process to increase the production of monascin (508.6 U/mL), and then systematically characterized its structure via HPLC, HPLC-MS, <sup>1</sup>H NMR, and <sup>13</sup>C NMR. Additionally, we innovatively modified carboxymethylcellulose sodium with selenium (CMC-Se) to encapsulate monascin (monascin@CMC-Se), which can sensitively respond to ROS and release monascin to effectively scavenge excessive ROS. Besides, the monascin@CMC-Se can significantly increase the bioaccessibility of monascin and alleviate cellular oxidative stress by enhancing its cellular uptake rate. Collectively, our work provides proof-of-principle evidence that the CMC-Se can precise delivery of monascin to an oxidatively stressed environment with high resistance to gastric fluids, laying a foundation to overcome inflammation-related diseases in the colon.</p>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143046881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbial protein production during fermentation of starch-rich legume flours using Aspergillus oryzae and Lactobacillus plantarum starter cultures.

IF 2.3 4区生物学

Journal of bioscience and bioengineering Pub Date : 2025-01-24 DOI: 10.1016/j.jbiosc.2024.12.015

Yuriy Kryachko, Lashmitha Arasaratnam, James Duncan House, Yongfeng Ai, Michael Todd Nickerson, Darren Raymond Korber, Takuji Tanaka

{"title":"Microbial protein production during fermentation of starch-rich legume flours using Aspergillus oryzae and Lactobacillus plantarum starter cultures.","authors":"Yuriy Kryachko, Lashmitha Arasaratnam, James Duncan House, Yongfeng Ai, Michael Todd Nickerson, Darren Raymond Korber, Takuji Tanaka","doi":"10.1016/j.jbiosc.2024.12.015","DOIUrl":"https://doi.org/10.1016/j.jbiosc.2024.12.015","url":null,"abstract":"<p><p>Starch-rich faba bean, yellow lentil, and yellow field pea flours were subjected to submerged fermentation using Aspergillus oryzae and Lactobacillus plantarum starter mono- or co-cultures, to increase protein contents of the flours. Fermentation mixes were supplemented with up to 35 g/L urea, ammonium sulfate and/or monoammonium phosphate as nitrogen sources. Protein contents of the flours increased 2-2.5-fold, i.e., total protein contents of up to 33 % were achieved following fermentation with A. oryzae monoculture or A. oryzae‒L. plantarum co-culture. Therefore, A. oryzae was capable of efficient legume starch utilization and protein production. Using A. oryzae‒L. plantarum co-culture led to the highest yields of at least six of microbially produced amino acids. After fermentation by L. plantarum monoculture, protein contents remained largely unchanged. However, in vitro protein digestibility of flours improved to a greater extent after fermentation with L. plantarum monoculture than with either A. oryzae monoculture or the co-culture. Hence, during fermentation with L. plantarum monoculture, microbially mediated hydrolysis of legume protein (generating smaller and easier digestible peptides), rather than microbial starch utilization, was the predominant process.</p>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143038863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Monitoring and optimization of the microenvironment in a gravity-driven microfluidic system placed on a slow-tilting table.

IF 2.3 4区生物学

Journal of bioscience and bioengineering Pub Date : 2025-01-21 DOI: 10.1016/j.jbiosc.2024.12.014

Nuttakrit Limjanthong, Shinji Sugiura, Taira Oda, Fuko Takusari, Yasunori Fujiwara, Toshimasa Miyazaki, Kosei Naganuma, Kiyoshi Ohnuma

{"title":"Monitoring and optimization of the microenvironment in a gravity-driven microfluidic system placed on a slow-tilting table.","authors":"Nuttakrit Limjanthong, Shinji Sugiura, Taira Oda, Fuko Takusari, Yasunori Fujiwara, Toshimasa Miyazaki, Kosei Naganuma, Kiyoshi Ohnuma","doi":"10.1016/j.jbiosc.2024.12.014","DOIUrl":"https://doi.org/10.1016/j.jbiosc.2024.12.014","url":null,"abstract":"<p><p>Gravity-driven microfluidic chips offer portability and flexibility in different settings because pumps and connecting tubes are unnecessary for driving fluid flow. In a previous study, human induced pluripotent stem cells were cultured using gravity-driven microfluidics, with the liquid flow rate regulated by a tilting table. However, instability in cell culture has been observed, occasionally leading to cell death owing to unknown causes. This study measured the ability of a gravity-driven microfluidic system to maintain essential microenvironments, specifically the flow rate, CO<sub>2</sub> levels, temperature, and humidity. The incubation procedure was improved to stabilize the parameters at target values. Improvements in the incubation process reduced the time required to reach the stabilized value for CO<sub>2</sub>, temperature, and humidity by 85, 67, and 5 %, respectively, compared to previous methods. The system demonstrated a precise flow rate, confirmed by a consistent increase in the downstream tank's medium volume after 4 h of perfusion. In addition, the adjustment of the tilting table maintained a steady angle and effectively regulated the flow rate, with the measured flow rate consistent with the theoretical value. The gravity-driven microfluidic system effectively facilitated the culture and differentiation of human iPSCs into the mesodermal lineage after bone morphogenetic protein 4 induction, as indicated by positive SSEA1 immunostaining, demonstrating its potential for stem cell research. Gravity-driven microfluidic systems satisfy these requirements and are suitable for stem cell culture experiments.</p>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143023592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0