Journal of bioscience and bioengineering最新文献

{"title":"Development of dectin-1-binding peptides targeting dendritic cells for antigen delivery via ribosome display","authors":"Yoshirou Kawaguchi ,&nbsp;Md Shahin Sarker ,&nbsp;Mina Yokoyama ,&nbsp;Misuzu Nakaya ,&nbsp;Takanatsu Hosokawa ,&nbsp;Noriho Kamiya ,&nbsp;Masahiro Goto","doi":"10.1016/j.jbiosc.2025.11.005","DOIUrl":"10.1016/j.jbiosc.2025.11.005","url":null,"abstract":"<div><div>Dendritic cells (DCs) are professional antigen-presenting cells that play a central role in initiating and shaping adaptive immune responses. Targeting antigens to DCs has emerged as a promising strategy to enhance vaccine efficacy and tailor desired immune responses. While antibodies are well established as targeting molecules for DCs, the use of peptides remains underexplored despite their favorable tissue penetration and their ability to offer design flexibility. Here, we report the identification of dectin-1-binding peptides using a ribosome display-based in vitro directed evolution system. Dectin-1 is a C-type lectin receptor expressed on murine CD11b⁺ and human CD1c⁺ DCs, which plays a key role in antigen uptake and in directing immune responses toward specific pathways, particularly the Th17 pathway. Using recombinant murine dectin-1 as bait, we performed four rounds of ribosome display selection and obtained peptides with high affinity. Selected peptides fused to enhanced green fluorescent protein showed binding to recombinant dectin-1 and native dectin-1-expressing cells, including bone marrow-derived DCs. These results demonstrated the feasibility of peptide-based molecular targeting toward dectin-1⁺ DCs, which would not only provide a versatile platform for the development of next-generation vaccines and immunotherapies, but also a valuable tool for dissecting the functional roles of dectin-1⁺ DCs in immune regulation.</div></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"141 3","pages":"Pages 158-164"},"PeriodicalIF":2.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145701096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recovering genomes from uncultured fungi with single-cell genomics","authors":"Nevin McCone ,&nbsp;Masahito Hosokawa","doi":"10.1016/j.jbiosc.2025.11.004","DOIUrl":"10.1016/j.jbiosc.2025.11.004","url":null,"abstract":"<div><div>Single-cell genomics (SCG) complements culture-independent metagenomics for accessing fungal genomes, particularly from lineages that remain uncultured. We contrast metagenomics, which excels when profiling community composition and metabolic potential but often underrepresents low-abundance fungi, with SCG, which first isolates individual cells or nuclei to generate single-amplified genomes (SAGs) and can recover rare or microdiverse taxa. We then organize existing fungal SCG applications into three subgroups: spore-level sequencing from host-enriched or environmental material; single-nucleus genomics for multinucleate fungi; and single-spore sequencing of haploid progeny for diploid linkage and chromosome phasing. Across studies, pooling and co-assembly of cognate cells improves completeness; key hurdles persist in wall lysis, whole-genome amplification bias, and contamination control. Practical advances include shallow sequencing for QC triage, nuclei pooling with normalized co-assembly, and hybrid long- and short-read assembly. SCG adds unique value where strain resolution and genotypic context matter, including host-to-mobile-element linkage, recovery of large biosynthetic gene clusters, and karyotype validation against telomere-to-telomere references. Used alongside metagenomics, SCG enables a strain-resolved view of fungal biodiversity and function, with incremental improvements across the SCG pipeline promising routine access to genomes from early-diverging and other environmentally embedded fungi.</div></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"141 3","pages":"Pages 143-151"},"PeriodicalIF":2.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145695859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functionalized peptide hydrogel to generate human insulin-producing cells in vitro","authors":"Brandhon F. Flores-Ibarra ,&nbsp;Andrea I. Enríquez-Rodríguez ,&nbsp;Kimberly P. Robles-Pablos ,&nbsp;Beatriz A. Rodas-Junco ,&nbsp;Waldo M. Argüelles-Monal ,&nbsp;Luisa L. Silva-Gutiérrez ,&nbsp;Refugio Pérez-González ,&nbsp;Olga A. Patrón-Soberano ,&nbsp;Carmen S. Rochín-Wong ,&nbsp;Luis A. Castillo-Díaz","doi":"10.1016/j.jbiosc.2025.11.007","DOIUrl":"10.1016/j.jbiosc.2025.11.007","url":null,"abstract":"<div><div>Type I diabetes is a chronic disease that affects people worldwide. When insulin administration is no longer effective, transplantation of pancreatic islets represents an alternative for diabetics. However, islet grafting carries limitations, which include poor availability of donors, surgery risks, and lifelong immunosuppressive therapy. To address this, novel approaches, such as the use of soft hydrogels as vehicles of cells are being developed for tissue grafting applications. Self-assembling peptide hydrogels (SAPHs) are biocompatible and versatile materials widely used for both, three-dimensional (3D) cell culture and regenerative medicine applications. Therefore, in this study, we explored the effect of the functionalization of the SAPH FEFEFKFKK (FEK9) with extracellular matrix (ECM) motifs, RGD, GFOGER and IKVAV, to support the directed differentiation of human dental pulp stem cells (hDPSCs) into insulin-producing cells (IPCs). The resulting ECM-functionalized FEK9 hydrogel was formed under mildly acidic conditions (pH 5–6). Infrared spectroscopy confirmed that ECM-FEK9 adopts a β-sheet secondary structure and forms a dense nanofibrillar network, while rheological measurements demonstrated the formation of a soft hydrogel. hDPSC cultured in hydrogel displayed steady viability and metabolism. Moreover, under directed induction, cells in ECM-FEK9 expressed β-cell markers, such as PDX-1 and Glut-2, as well as synthetized insulin within 10 days of 3D culture <em>in vitro</em>, as evidenced through fluorescence confocal microscopy and spectrophotometry evaluations, respectively. Therefore, ECM-FEK9 could be a promising candidate to support the culture of hDPSCs and the generation of IPCs after refinement of directed induction under 3D cell culture conditions.</div></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"141 3","pages":"Pages 185-193"},"PeriodicalIF":2.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145819421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adaptive laboratory evolution optimizes an engineered phosphite utilization pathway in Synechococcus elongatus PCC 7942","authors":"Hiroki Murakami ,&nbsp;Naoki Momokawa ,&nbsp;Kei Motomura ,&nbsp;Akio Kuroda ,&nbsp;Ryuichi Hirota","doi":"10.1016/j.jbiosc.2025.11.006","DOIUrl":"10.1016/j.jbiosc.2025.11.006","url":null,"abstract":"<div><div>Synthetic biology approaches enable the creation of promising chassis for practical application in various fields, though engineering of microbial metabolism often imposes a metabolic burden, potentially driving adaptive evolution during long-term cultivation. A previously established phosphite (Pt)-dependent metabolic system has proven to be an effective strategy for the containment of genetically engineered microorganisms, although its implementation accompanied a slight growth retardation. Here, we investigated the effect of long-term serial passaging cultivation on the Pt-dependent strain of <em>Synechococcus elongatus</em> PCC 7942, RH714. Compared with the originally constructed RH714, the passaged population of RH714 exhibited improved growth and a higher rate of Pt consumption in culture medium. Sequence analysis revealed point mutations within the introduced <em>htxBCDE</em> transporter genes, which are required for selective incorporation of Pt as a phosphorus nutrition. Introduction of the mutated gene cluster into <em>S. elongatus</em> PCC 7942 reproduced the traits of the passaged RH714 population, suggesting that these genetic changes enhance Pt transport activity and account for the observed phenotypes. Disruption of endogenous phosphate (Pi) transporter genes in the strains expressing the mutated <em>htxBCDE-ptxD</em> cluster abolished growth in Pi-containing medium, suggesting that the mutations in the transporter genes did not alter substrate specificity toward Pi. These results indicated that long-term passage cultivation developed an optimized mutant capable of efficient proliferation under the Pt metabolizing conditions without compromising its biocontainment capability.</div></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"141 3","pages":"Pages 152-157"},"PeriodicalIF":2.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145751920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sequential active learning for medium optimization in mAb production","authors":"Takamasa Hashizume ,&nbsp;Koki Baba ,&nbsp;Naoya Matsuo ,&nbsp;Bei-Wen Ying","doi":"10.1016/j.jbiosc.2025.12.002","DOIUrl":"10.1016/j.jbiosc.2025.12.002","url":null,"abstract":"<div><div>Monoclonal antibodies (mAbs) are key therapeutics for diseases like cancer and autoimmunity. The production of mAbs relies on cell culture, in which the culture medium for high productivity and activity is essential. Despite the traditional manual and advanced computational methodologies for medium optimization, it remains challenging to incorporate biological insights gained during cell culture experimentation into the optimization process. To address this issue, an active learning strategy that sequentially integrates machine learning predictions with experimental observations of biological meaningfulness was developed in the present study. Medium design and prediction were conducted with the combination of the design of experiment and two different machine learning models, to optimize the culture medium for Chinese hamster ovary (CHO) cells producing increased immunoglobulin G (IgG) titer. Using this approach, we iteratively adjusted the concentrations of 44 components in a serum-free medium and achieved a significant improvement in IgG monoclonal antibody production. Biological insights such as osmolality control and amino acid composition, which were not initially considered, were progressively incorporated into the data-driven optimization process. The proposed strategy is practical and effective, even under limited experimental resources, and offers a new direction for rational medium design in biopharmaceutical manufacturing.</div></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"141 3","pages":"Pages 210-220"},"PeriodicalIF":2.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145850052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spheroid size-induced apoptosis enhances osteogenic differentiation of iPS cells","authors":"Hideto Tatsumi ,&nbsp;Hiroko Okawa ,&nbsp;Naruephorn Vinaikosol ,&nbsp;Akihito Moribayashi ,&nbsp;Hiroki Kayashima ,&nbsp;Hirofumi Yatani ,&nbsp;Hiroshi Egusa","doi":"10.1016/j.jbiosc.2025.11.010","DOIUrl":"10.1016/j.jbiosc.2025.11.010","url":null,"abstract":"<div><div>Induced pluripotent stem cells (iPSCs) have significant potential for regenerative medicine, particularly for bone tissue engineering. While three-dimensional spheroid cultures enhance iPSC differentiation by better mimicking physiological conditions, spheroid size critically affects cell viability and differentiation ability. Microwell plates enable large-scale production of uniform spheroids and would be especially useful for regenerative medicine and tissue engineering. Here, we investigated the effect of spheroid size on the osteogenic differentiation of iPSCs using microwell plates to generate spheroids under the following conditions: Elp200 (microwell plate with 200/100 μm diameter/depth) and Elp900 (microwell plate with 900/700 μm). We observed that larger Elp900 spheroids promoted mesodermal differentiation more effectively, likely due to enhanced cell–cell interactions and altered internal microenvironments. However, Elp900 spheroids exhibited increased apoptosis in their core regions, evidenced by viability staining, transmission electron microscopy, and TUNEL staining. Upon dissociation and adherent culture, Elp900-derived cells demonstrated significantly higher expression of osteogenic markers (<em>Runx2</em>, <em>Ibsp</em>) and mineralization compared to Elp200-derived cells. Proteomic analysis revealed that apoptosis- and extracellular matrix (ECM)-related proteins, such as SERPINH1 and COL4A1, were upregulated in Elp900 cultures. These findings suggest that controlled apoptosis within larger spheroids may activate stress-related pathways, promote ECM formation, and enhance osteogenic differentiation by activating the TGF-β signaling pathway. Our findings highlight optimal spheroid sizing as a key factor for maximizing the efficiency and reproducibility of osteogenic differentiation of iPSCs, providing a foundation for improved strategies in iPSC-based bone tissue regeneration.</div></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"141 3","pages":"Pages 203-209"},"PeriodicalIF":2.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145804722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aerobic degradation characteristics of cis-1,2-dichloroethene by Pseudonocardia sp. D17: Degradation kinetics, putative degradation pathways, and involvement of soluble di-iron monooxygenases in the initial oxidation","authors":"Ryugo Nishimine ,&nbsp;Yuna Kaneko ,&nbsp;Shinpei Fujiwara ,&nbsp;Daisuke Inoue ,&nbsp;Masahiro Takeo ,&nbsp;Michihiko Ike","doi":"10.1016/j.jbiosc.2025.10.012","DOIUrl":"10.1016/j.jbiosc.2025.10.012","url":null,"abstract":"<div><div><em>Pseudonocardia</em> sp. D17 (D17) is a novel strain capable of aerobically metabolizing <em>cis</em>-1,2-dichloroethene (cDCE), a persistent contaminant in soil and groundwater. This study aimed to investigate the cDCE degradation characteristics of D17 with respect to kinetics, associated degradative enzymes, and degradation pathways. Degradation experiments with cDCE concentrations ranging from 0.267 to 91.3 μM revealed that D17 can efficiently degrade cDCE across this range. The maximum specific degradation rate and half saturation constant for cDCE degradation by D17 were estimated to be 0.418 ± 0.045 nmol/mg-protein/min and 38.5 ± 9.2 μM, respectively. Heterologous expression experiments demonstrated that both group 5 soluble di-iron monooxygenases in D17, namely tetrahydrofuran and propane monooxygenases, can catalyze cDCE degradation with higher catalytic activity observed in the former. This suggests their involvement in cDCE degradation by D17. It was also proposed that D17 completely dechlorinates cDCE through multiple pathways to generate glyoxylic acid, which is either mineralized or incorporated into the glyoxylate cycle, with a minor portion being converted to oxalic acid as a dead-end product. These findings provide novel insights into metabolic aerobic cDCE biodegradation and highlight the potential of D17 as a bioremediation agent.</div></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"141 2","pages":"Pages 116-124"},"PeriodicalIF":2.9,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145512940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Using pure oxygen aeration to increase recombinant protein production by an Aspergillus oryzae hyphal dispersion strain","authors":"Satoshi Ishibashi ,&nbsp;Shunya Susukida ,&nbsp;Kiyoaki Muto ,&nbsp;Ken Miyazawa ,&nbsp;Silai Zhang ,&nbsp;Akira Yoshimi ,&nbsp;Eiji Tabata ,&nbsp;Keietsu Abe","doi":"10.1016/j.jbiosc.2025.10.011","DOIUrl":"10.1016/j.jbiosc.2025.10.011","url":null,"abstract":"<div><div>A high growth rate is essential for increasing protein production efficiency in liquid fermentation of filamentous fungi, such as <em>Aspergillus oryzae</em>. However, the increase in culture viscosity due to fungal growth constrains the overall yield. We have demonstrated that culture viscosity is lower in <em>A. oryzae</em> AGΔ-GAGΔ strains, which are deficient in the cell surface polysaccharides α-1,3-glucan (AG) and galactosaminogalactan (GAG), than in the wild-type (WT) strains. Nevertheless, even in aerated fermentation, an increase in AGΔ-GAGΔ viscosity results in oxygen depletion, which limits fungal growth and enzyme production. In this study, we investigated viscosity dynamics and protein production during high-cell-density fermentation of AGΔ-GAGΔ under pure oxygen aeration. Fed-batch cultivation of the WT and AGΔ-GAGΔ strains, expressing recombinant xylanase (XynF1), was used to compare the effects of air and pure oxygen aeration at the same flow rate. At 60 h, AGΔ-GAGΔ under pure oxygen aeration showed higher cell density (1.2× WT under pure oxygen aeration, 2.1× AGΔ-GAGΔ under air aeration) and XynF1 activity (1.8× WT under pure oxygen aeration, 2.3× AGΔ-GAGΔ under air aeration). Under pure oxygen aeration, AGΔ-GAGΔ showed lower viscosity (0.32×) and mixing time (0.50×) than WT. At 60 h, fine mycelial pellets (micropellets; 200–700 μm) were clearly observed in AGΔ-GAGΔ under pure oxygen but not under air aeration. These findings suggest that oxygen enrichment during AGΔ-GAGΔ cultivation mitigated the increase in viscosity, thereby promoting higher cell density and protein production. The formation of micropellets in AGΔ-GAGΔ likely contributed to a reduction in culture viscosity.</div></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"141 2","pages":"Pages 98-107"},"PeriodicalIF":2.9,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145604529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"One-pot synthesis of β-alanine from 1,3-diaminopropane using two-enzyme cascade in cell-free biotransformation","authors":"Shanthi Shanmugasundaram,&nbsp;Anitha Janet Roshni Yesudhas,&nbsp;Andrea Kagoo,&nbsp;Ramalingam Subramanian","doi":"10.1016/j.jbiosc.2025.10.013","DOIUrl":"10.1016/j.jbiosc.2025.10.013","url":null,"abstract":"<div><div>A novel two-enzyme cascade one-pot synthesis of β-alanine from 1,3-diaminopropane (DAP) has been developed. In the first step, DAP was oxidized to 3-aminopropionaldehyde (3-APAL) by diamine oxidase (DAO). In the second step, 3-APAL was oxidized to β-alanine by 3-APAL dehydrogenase (APALDH). Catalase and NADH oxidase were employed to degrade the by-product, H₂O₂, and to regenerate the cofactor, NAD⁺. DAO specific to DAP has not been reported in any prokaryote. Therefore, initial proof of concept was established using commercial eukaryotic DAO_pk (from porcine kidney) and catalase (from bovine liver), along with recombinant APALDH and NOX enzymes sourced from <em>Arthrobacter crystallopoietes</em> and <em>Lactococcus lactis</em>, respectively. β-Alanine was successfully produced via this pathway; 12.2 mM (1.1 g/L) was formed in 41 h with 12 % conversion. To increase the reaction rate, DAO with higher specific activity was identified from <em>Arthrobacter pascens</em> (DAO_Ap). The optimum pH and temperature of DAO_Ap were determined to be 9.0 and 37 °C, respectively. Batch enzymatic biotransformation achieved 6 % conversion, yielding 0.33 mM (29 mg/L) β-alanine in 4 h. The low titre in batch conversion was attributed to substrate inhibition affecting DAO_Ap, NOX, and catalase. Fed-batch enzymatic biotransformation was conducted to overcome substrate inhibition, yielding 47 % conversion, with 2.34 mM (63 mg/L) β-alanine formation in 4 h. Approximately a 7.5-fold increase in conversion was achieved using fed-batch enzymatic biotransformation. This study accomplished a novel two-enzyme cascade biotransformation strategy for one-pot β-alanine synthesis from DAP.</div></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"141 2","pages":"Pages 90-97"},"PeriodicalIF":2.9,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145549243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biodegradation of recalcitrant environmental pollutants by white-rot fungi","authors":"Mingdong Chang ,&nbsp;Ru Yin ,&nbsp;Jianqiao Wang ,&nbsp;Nana Wang ,&nbsp;Pengfei Xiao","doi":"10.1016/j.jbiosc.2025.10.010","DOIUrl":"10.1016/j.jbiosc.2025.10.010","url":null,"abstract":"<div><div>The understanding of white-rot fungi (WRF) and their role in degrading recalcitrant environmental pollutants has significantly advanced due to developments in bioremediation research. Considerable progress has been made in elucidating the degradation capabilities of WRF against lots of environmental pollutants. In this review, research hotspots on the degradation of WRF were identified through bibliometric analysis. Key findings from systematic studies on the degradation of polycyclic aromatic hydrocarbons (PAHs) and bisphenols by WRF are synthesized and discussed. Furthermore, insights into the molecular and genetic basis underlying the enzymatic systems responsible for the degradation of PAHs and bisphenols are highlighted. Advancements and challenges in understanding the degradation capabilities and degradation mechanisms are examined in order to identify opportunities for developing more effective strategies to harness the bioremediation potential of WRF.</div></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"141 2","pages":"Pages 81-89"},"PeriodicalIF":2.9,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145549307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}