Journal of bioscience and bioengineering最新文献_第8页

Individual evaluation of nitrite and free nitrous acid inhibition on anammox activity 亚硝酸盐和游离亚硝酸对 Anammox 活性抑制作用的单独评估。

IF 2.3 4区生物学

Journal of bioscience and bioengineering Pub Date : 2024-08-05 DOI: 10.1016/j.jbiosc.2024.06.010

Koya Hirose , Takashi Kondo , Yayoi Saito , Kazuichi Isaka

{"title":"Individual evaluation of nitrite and free nitrous acid inhibition on anammox activity","authors":"Koya Hirose , Takashi Kondo , Yayoi Saito , Kazuichi Isaka","doi":"10.1016/j.jbiosc.2024.06.010","DOIUrl":"10.1016/j.jbiosc.2024.06.010","url":null,"abstract":"<div>The anammox reaction simultaneously utilizes ammonia and nitrite as substrates; however, high nitrite concentrations act as strong inhibitors of the reaction. In this study, inhibition by NO2− and free nitrous acid (FNA) was separately evaluated in continuous feeding tests using different biomass carriers. The influent NO2− concentration was increased under pH 7.6, where FNA is less likely to affect anammox activity. A continuous test using polyethylene glycol (PEG) gel carriers containing immobilized anammox bacteria showed that the inhibition ratio was 13% when the NO2−-N concentration in the reactor was 350 mg L−1 (FNA ≤0.06 mg L−1). The relationship between NO2− concentration in the reactor and inhibition ratio increased linearly. Evaluation of the inhibitory effect of FNA by increasing the influent NO2− concentration at pH 6.4, where FNA is easily formed, demonstrated that the relationship between FNA and inhibition ratio could be fitted to a sigmoid curve, and the 50% inhibitory concentration (IC50) of FNA was 0.88 mg L−1. A similar test performed using polyvinyl alcohol carriers containing anammox bacteria on their surface showed the same trend as the PEG gel carriers, with the IC50 for FNA at 0.70 mg L−1. These results indicate that the inhibitory effect of FNA on anammox activity was greater than that of NO2−. The evaluation of these two factors helped identify important operational indicators of the stable application of anammox processes.</div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"138 4","pages":"Pages 345-350"},"PeriodicalIF":2.3,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141897530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Physiological responses contributing to multiple stress tolerance in Pichia kudriavzevii with potential enhancement for ethanol fermentation 有助于提高 Pichia kudriavzevii 对多种胁迫耐受性的生理反应，并有可能提高其乙醇发酵能力。

IF 2.3 4区生物学

Journal of bioscience and bioengineering Pub Date : 2024-08-03 DOI: 10.1016/j.jbiosc.2024.07.012

Pongsanat Pongcharoen , Wittaya Tawong , Wanwarang Pathaichindachote , Weerawan Rod–in

{"title":"Physiological responses contributing to multiple stress tolerance in Pichia kudriavzevii with potential enhancement for ethanol fermentation","authors":"Pongsanat Pongcharoen , Wittaya Tawong , Wanwarang Pathaichindachote , Weerawan Rod–in","doi":"10.1016/j.jbiosc.2024.07.012","DOIUrl":"10.1016/j.jbiosc.2024.07.012","url":null,"abstract":"<div>Economically feasible ethanol production requires efficient hydrolysis of lignocellulosic biomass and high–temperature processing to enable simultaneous saccharification and fermentation. During the lignocellulolysic hydrolysate, the yeast must encounter with a multiple of inhibitors such as heat and furfural. To solve this problem, a potential fermentative yeast strain that tolerated simultaneous multistress and enhance ethanol concentration was investigated. Twenty yeast isolates were classified into two major yeast species, namely Pichia kudriavzevii (twelve isolates) and Candida tropicalis (eight isolates). All P. kudriavzevii isolates were able to grow at high temperature (45 °C) and exhibited stress tolerance toward furfural. Among P. kudriavzevii isolates, NUCG–S3 presented the highest specific growth rate under each stress condition of heat and furfural, and multistress. Morphological changes in P. kudriavzevii isolates (NUCG–S2, NUCG–S3, NUKL–P1, NUKL–P3, and NUOR–J1) showed alteration in mean cell length and width compared to the non–stress condition. Ethanol production by glucose was also determined. The yeast strain, NUCG–S3, gave the highest ethanol concentrations at 99.46 ± 0.82, 62.23 ± 0.96, and 65.80 ± 0.62 g/l (P < 0.05) under temperature of 30 °C, 40 °C, and 42 °C, respectively. The tolerant isolated yeast NUCG–S3 achieved ethanol production of 53.58 ± 3.36 and 48.06 ± 3.31 g/l (P < 0.05) in the presence of 15 mM furfural and multistress (42 °C with 15 mM furfural), respectively. Based on the results of the present study, the novel thermos and furfural-tolerant yeast strain P. kudriavzevii NUCG–S3 showed promise as a highly proficient yeast for high–temperature ethanol fermentation.</div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"138 4","pages":"Pages 314-323"},"PeriodicalIF":2.3,"publicationDate":"2024-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141889344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regioselective photocyclodimerization of 2-anthracenecarboxylic acid through ATP hydrolysis-driven conformational change using simulation prediction-designed GroEL mutant 利用模拟预测设计的 GroEL 突变体，通过 ATP 水解驱动构象变化，实现 2-蒽羧酸的区域选择性光环二聚化。

IF 2.3 4区生物学

Journal of bioscience and bioengineering Pub Date : 2024-08-02 DOI: 10.1016/j.jbiosc.2024.07.002

Masaki Nishijima , Kota Kobayashi , Megumi Masuda-Endo , Hiromi Yoda , Ayumi Koike-Takeshita

引用次数: 0

Peptide array screening with anti-GLP-1 monoclonal antibody: Discovery of cysteine-containing DPP-IV inhibitory peptides 用抗 GLP-1 单克隆抗体进行肽阵列筛选：发现含半胱氨酸的 DPP-IV 抑制肽。

IF 2.3 4区生物学

Journal of bioscience and bioengineering Pub Date : 2024-07-31 DOI: 10.1016/j.jbiosc.2024.07.001

Masaki Kurimoto , Naoki Yuda , Masayoshi Tanaka , Miyuki Tanaka , Mina Okochi

{"title":"Peptide array screening with anti-GLP-1 monoclonal antibody: Discovery of cysteine-containing DPP-IV inhibitory peptides","authors":"Masaki Kurimoto , Naoki Yuda , Masayoshi Tanaka , Miyuki Tanaka , Mina Okochi","doi":"10.1016/j.jbiosc.2024.07.001","DOIUrl":"10.1016/j.jbiosc.2024.07.001","url":null,"abstract":"<div>Inhibition of dipeptidyl peptidase IV (DPP-IV) is an effective pharmacotherapy for the management of type 2 diabetes. Recent findings have suggested that various dietary proteins can serve as precursors to peptides that inhibit DPP-IV. Although several DPP-IV inhibitory peptides derived from food materials have been reported, more effective inhibitory peptides remain to be discovered. This study aimed to identify potent DPP-IV inhibitory peptides that earlier approaches had overlooked by employing a screening method that combined peptide arrays and neutralizing antibodies. Octa-peptides covering the complete amino acid sequences of four casein proteins and two whey proteins were synthesized on arrays via a solid-phase method. These peptides were then reacted with a monoclonal antibody specifically engineered to recognize glucagon-like peptide 1 (GLP-1), a substrate of DPP-IV. The variable region of the anti-GLP-1 monoclonal antibody is utilized to mimic the substrate-binding region of DPP-IV, enabling the antibody to bind to peptides that interact with DPP-IV. Based on this feature, 26 peptides were selected as DPP-IV inhibitory peptide candidates, 11 of which showed strong DPP-IV inhibitory activity. Five of these peptides consistently contained cysteines positioned two to four residues from the N-terminus. Treatment with disulfide formation decreased the DPP-IV inhibitory activity of these cysteine-containing peptides, while the inhibitory activity of α-lactalbumin hydrolysates increased with reducing treatment. These results revealed that the thiol group is important for DPP-IV inhibitory activity. This study provides a useful screen for DPP-IV inhibitory peptides and indicates the importance of reductive cysteine residues within DPP-IV inhibitory peptides.</div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"138 4","pages":"Pages 351-359"},"PeriodicalIF":2.3,"publicationDate":"2024-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141859879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation of arginine deiminase system-deficient mutants of Tetragenococcus halophilus using arginine analog canavanine 利用精氨酸类似物卡纳瓦宁分离嗜卤四源球菌精氨酸脱氨酶系统缺陷突变体

IF 2.3 4区生物学

Journal of bioscience and bioengineering Pub Date : 2024-07-29 DOI: 10.1016/j.jbiosc.2024.07.006

Takura Wakinaka , Jun Watanabe , Yoshinobu Mogi

引用次数: 0

Rational design approach to improve the solubility of the β-sandwich domain 1 of a thermophilic protein 提高嗜热蛋白质β-三明治结构域1溶解度的合理设计方法。

IF 2.3 4区生物学

Journal of bioscience and bioengineering Pub Date : 2024-07-29 DOI: 10.1016/j.jbiosc.2024.06.009

Chukwuebuka M. Ononugbo , Yusaku Shimura , Noriko Yamano-Adachi , Takeshi Omasa , Yuichi Koga

{"title":"Rational design approach to improve the solubility of the β-sandwich domain 1 of a thermophilic protein","authors":"Chukwuebuka M. Ononugbo , Yusaku Shimura , Noriko Yamano-Adachi , Takeshi Omasa , Yuichi Koga","doi":"10.1016/j.jbiosc.2024.06.009","DOIUrl":"10.1016/j.jbiosc.2024.06.009","url":null,"abstract":"<div>The β-sandwich domain 1 (SD1) of islandisin is a stable thermophilic protein with surface loops that can be redesigned for specific target binding, architecturally comparable to the variable domain of immunoglobulin (IgG). SD1's propensity to aggregate due to incorrect folding and subsequent accumulation in Escherichia coli inclusion bodies limits its use in biotechnological applications. We rationally designed SD1 for improved variants that were expressed in soluble forms in E. coli while maintaining the intrinsic thermal stability of the protein (melting temperature (Tm) = 73). We used FoldX's ΔΔG predictions to find beneficial mutations and aggregation-prone regions (APRs) using Tango. The S26K substitution within protein core residues did not affect protein stability. Among the soluble mutants studied, the S26K/Q91P combination significantly improved the expression and solubility of SD1. We also examined the effects of the surface residue, pH, and concentration on the solubility of SD1. We showed that the surface polarity of proteins had little or no effect on solubility, whereas surface charges played a substantial role. The storage stability of several SD1 variants was impaired at pH values near their isoelectric point, and pH levels resulting in highly charged groups. We observed that mutations that create an uneven distribution of charged groups on the SD1 surface could enhance protein solubility by eliminating favorable protein–protein surface charge interactions. Our findings suggest that SD1 is mutationally tolerant to new functionalities, thus providing a novel perspective for the application of rational design to improve the solubility of targeted proteins.</div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"138 4","pages":"Pages 271-282"},"PeriodicalIF":2.3,"publicationDate":"2024-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141792523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Beneficial base substitutions in Escherichia coli fucO gene for enhancement of glycolic acid production 在大肠杆菌 fucO 基因中进行有益的碱基替换，以提高乙醇酸的产量。

IF 2.3 4区生物学

Journal of bioscience and bioengineering Pub Date : 2024-07-29 DOI: 10.1016/j.jbiosc.2024.06.007

Mayu Nemoto , Wataru Muranushi , Chen Shuting , Yusuke Saito , Daisuke Sugimori , Miwa Yamada

{"title":"Beneficial base substitutions in Escherichia coli fucO gene for enhancement of glycolic acid production","authors":"Mayu Nemoto , Wataru Muranushi , Chen Shuting , Yusuke Saito , Daisuke Sugimori , Miwa Yamada","doi":"10.1016/j.jbiosc.2024.06.007","DOIUrl":"10.1016/j.jbiosc.2024.06.007","url":null,"abstract":"<div>Microbial production of glycolic acid (GA) from ethylene glycol is extensively used in a variety of industries because ethylene glycol is not only an inexpensive raw material but also the main component of industrial wastes. In this study, we produced GA from ethylene glycol using Escherichia coli overexpressing the endogenous 1,2-propanediol oxidoreductase (fucO) and lactaldehyde dehydrogenase (aldA) genes. To increase GA productivity, we screened a random mutant library generated using an error-prone polymerase chain reaction of fucO and obtained FucO mutants MF2-9 and MF6-9 with enhanced GA production in Lysogeny Broth medium containing 800 mM ethylene glycol. MF2-9 contained three amino acid substitutions (D23E, E222K, and G363S) and two synonymous mutations (coding DNA [c.] 93G > A and c.1131T > C) in fucO. MF6-9 contained one amino acid substitution (L377H) in FucO. An amino acid substitution (L377H) and a single synonymous mutation (c.1131T > C) in fucO contributed to the enhancement in GA production. Notably, cell lysates from E. coli harboring a synonymous mutation (c.1131T > C) or amino acid substitution (L377H) in fucO showed that only AldA activity was 1.3-fold higher than that of the cell lysate from E. coli harboring the wild-type fucO. We confirmed that c.1131T > C and L377H mutations increased aldA expression in E. coli. Analysis of mRNA levels and simulation of mRNA stabilization indicated that base substitutions at positions c.1130T, which corresponds to L377H amino acid substitution, and c.1131T increased aldA expression due to partial destabilization of the mRNA. These findings will be useful for the large-scale microbial production of GA from industrial waste.</div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"138 4","pages":"Pages 301-307"},"PeriodicalIF":2.3,"publicationDate":"2024-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141855567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation of aflatoxin biosynthetic inhibitor from Chondrostereum purpureum mushroom culture filtrate 从软玉菇培养滤液中分离出黄曲霉毒素生物合成抑制剂。

IF 2.3 4区生物学

Journal of bioscience and bioengineering Pub Date : 2024-07-26 DOI: 10.1016/j.jbiosc.2024.07.003

Yuya Matsuno , Naoki Endo , Kotomi Ueno , Atsushi Ishihara

{"title":"Isolation of aflatoxin biosynthetic inhibitor from Chondrostereum purpureum mushroom culture filtrate","authors":"Yuya Matsuno , Naoki Endo , Kotomi Ueno , Atsushi Ishihara","doi":"10.1016/j.jbiosc.2024.07.003","DOIUrl":"10.1016/j.jbiosc.2024.07.003","url":null,"abstract":"<div>Aflatoxins (AFs) are highly toxic mycotoxins produced by the fungi, Aspergillus flavus and Aspergillus parasiticus. AFs pose severe health risks owing to their acute toxicity and carcinogenic properties. The control of AF contamination remains significantly challenging despite the extensive efforts toward controlling it. Here, we investigated the potential of mushroom extracts as a source of AF biosynthetic inhibitors. The A. parasiticus mutant strain, NFRI-95, that accumulates an AF biosynthesis intermediate, norsolorinic acid, was used in the bioassay to detect the inhibitory activity against AF biosynthesis. The screening of 195 mushroom extracts revealed that the culture filtrate extract of Chondrostereum purpureum exhibited strong inhibitory activity against AF biosynthesis. Next, large-scale culturing of C. purpureum was performed to isolate the compounds accounting for the inhibitory activity. The culture filtrate was extracted with ethyl acetate, after which the active compound was isolated by silica gel column chromatography and preparative high performance liquid chromatography (HPLC). The active compound was identified as cyclo(Val–Pro) by spectroscopic analyses. Further, four stereoisomers of cyclo(Val–Pro) were synthesized by the condensation of the N-Boc derivatives of d- and l-valine with the methyl esters of d- and l-proline. The naturally isolated compound was identified as cyclo(l-Val-l-Pro) by comparing its retention time with those of synthetic compounds by chiral HPLC analysis and CD spectra. The IC50 value of cyclo(L-Val-L-Pro) was 2.4 mM, whereas the LD, DL, and DD isomers exhibited weaker activities, with IC50 values of >5 mM.</div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"138 4","pages":"Pages 308-313"},"PeriodicalIF":2.3,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141788128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolated hair bacteria reveal different isolation possibilities under various conditions 分离出的毛发细菌显示了在不同条件下的不同分离可能性。

IF 2.3 4区生物学

Journal of bioscience and bioengineering Pub Date : 2024-07-20 DOI: 10.1016/j.jbiosc.2024.06.003

Azusa Yamada , Yuri Nishi , Mei Noguchi , Kota Watanabe , Mugihito Oshiro , Kenji Sakai , Yukihiro Tashiro

{"title":"Isolated hair bacteria reveal different isolation possibilities under various conditions","authors":"Azusa Yamada , Yuri Nishi , Mei Noguchi , Kota Watanabe , Mugihito Oshiro , Kenji Sakai , Yukihiro Tashiro","doi":"10.1016/j.jbiosc.2024.06.003","DOIUrl":"10.1016/j.jbiosc.2024.06.003","url":null,"abstract":"<div>Microorganisms are assumed to inhabit various environments and organisms, including the human body. The presence of more than 700 bacterial species on scalp hair has been reported through rRNA gene amplicon analysis. However, the biological properties of bacteria on the scalp hair (hair bacteria) and their functions are poorly understood as few hair bacteria have been isolated from hair in previous studies. This study aimed to isolate hair bacteria using standard media under 24 different conditions (including medium components, component concentrations, gelling agents, and atmospheric environments). Furthermore, we evaluated the possibility of isolating strains under these isolation conditions and examined the carbon metabolic ability of several predominantly isolated strains. A total of 63 bacterial species belonging to 27 genera were isolated from hair under 24 isolation conditions. The predominant bacterial species isolated from human hair in this study showed different carbon metabolic capabilities than those of the reference strains. In addition, isolation possibility was newly proposed to systematically evaluate the number of isolation conditions that could cultivate a bacterial species. Based on isolation possibility, the isolates were categorized into groups with a high number of isolation conditions (e.g., ≥25%; such as Staphylococcus) and those with a low number (e.g., ≤25%; such as Brachybacterium). These findings indicate the existence of easily isolated microorganisms and difficultly isolated microorganism from human hair.</div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"138 4","pages":"Pages 290-300"},"PeriodicalIF":2.3,"publicationDate":"2024-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141734182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of humic acid on aerobic denitrification by Achromobacter sp. strain GAD-3 腐殖酸对 Achromobacter sp.

IF 2.3 4区生物学

Journal of bioscience and bioengineering Pub Date : 2024-07-18 DOI: 10.1016/j.jbiosc.2024.06.008

Mengyao Gui

{"title":"Effect of humic acid on aerobic denitrification by Achromobacter sp. strain GAD-3","authors":"Mengyao Gui","doi":"10.1016/j.jbiosc.2024.06.008","DOIUrl":"10.1016/j.jbiosc.2024.06.008","url":null,"abstract":"<div>Humic acid (HA), a common natural organic matter, could affect conventional anoxic denitrification. Aim of this study was to investigate effect of HA on the process of aerobic denitrification in Achromobacter sp. GAD-3, an aerobic denitrifying strain. The findings demonstrated that an increase in HA concentrations (≥5 mg L−1) promoted the aerobic denitrification process (excluding N2O reduction), manifesting as higher rates of nitrate removal (6.67–11.1 mg L−1 h−1) and lower levels of nitrite accumulation (30.2–20.7 mg L−1). This was attributed to the increased electron transfer activities and denitrifying reductase activities (including NAR, NIR and NOR) facilitated by HA. Accordingly, the expression of denitrification genes such as napA, cnorB, and nirS was enhanced by HA. Nonetheless, the nosZ gene and N2OR activity underwent suppression by HA, which was accountable for N2O emission. It is crucial to understand the HA mechanism towards aerobic denitrifiers for wastewater treatment plants to enhance nitrogen removal.</div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"138 4","pages":"Pages 338-344"},"PeriodicalIF":2.3,"publicationDate":"2024-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141727289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0