加维氏乳球菌（Lactococcus garvieae）ABG0038 产生的一种环状细菌毒素加维素 SC（一种新型加维素 ML 变体）的鉴定和特征描述。

IF 2.3 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of bioscience and bioengineering Pub Date : 2024-11-15 DOI:10.1016/j.jbiosc.2024.10.008

Yumi Komori, Naoya Ozawa, Hiroshi Kuwahara, Takeshi Zendo, Mikio Aoki

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引用次数: 0

摘要

我们发现并鉴定了一种从松果中分离出来的加维叶乳球菌 ABG0038 产生的环状细菌素，称为加维素 SC（GarSC）。对L. garvieae ABG0038的基因组分析表明，GarSC是环状细菌素Garvicin ML（GarML）的一个变体，由一个氨基酸置换引起，并预测GarSC是通过与GarML非常相似的生物合成机制产生的。对这两种环状细菌素进行了纯化和活性鉴定，发现它们在 pH 值稳定性、酶敏感性和抗菌活性方面存在一些差异。特别是，GarSC 在碱性 pH 值范围内表现出优异的稳定性，这可能会扩大加维素的应用范围，使其比 GarML 的应用范围更广。

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Identification and characterization of a circular bacteriocin, garvicin SC, a novel garvicin ML variant, produced by Lactococcus garvieae ABG0038.

We have identified and characterized a circular bacteriocin, termed garvicin SC (GarSC), produced by Lactococcus garvieae ABG0038 isolated from pine cones. Genome analysis of L. garvieae ABG0038 revealed that GarSC was a variant of the circular bacteriocin, garvicin ML (GarML), caused by an amino acid substitution, and predicted that GarSC was produced through a biosynthetic mechanism very similar to that of GarML. The two circular bacteriocins were purified and characterized for activity, and several differences were observed in pH stability, enzyme sensitivity, and antimicrobial activity. In particular, GarSC showed excellent stability in the basic pH range, which might extend the range of garvicin's application to one broader than that of GarML.

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来源期刊

Journal of bioscience and bioengineering 生物-生物工程与应用微生物

CiteScore

5.90

自引率

3.60%

发文量

144

审稿时长

51 days

期刊介绍： The Journal of Bioscience and Bioengineering is a research journal publishing original full-length research papers, reviews, and Letters to the Editor. The Journal is devoted to the advancement and dissemination of knowledge concerning fermentation technology, biochemical engineering, food technology and microbiology.