Miriam Guadalupe Salgado García, Néstor Fabián Díaz, Guadalupe García López, Ikuri Álvarez Maya, Claudia Hernández Jimenez, Yvonne Roman Maldonado, David José Mendoza Aguayo, Néstor Emmanuel Díaz Martínez
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引用次数: 0
摘要
组织工程是一个旨在彻底改变再生医学的多学科研究领域，它依赖于支架来实现最佳的细胞培养和器官发育。脱细胞组织细胞外基质（dECM）支架，尤其是人羊膜（hAM）支架在临床应用中大有可为。这篇综述讨论了支架的意义，强调了基于 dECM 的 hAM 支架，深入探讨了 ECM 的复杂性、脱细胞过程和评估方法。拉曼光谱是评估 ECM 保存情况的一种非破坏性工具，具有量化脱细胞前后 hAM 中 ECM 成分的潜力。这篇综述探讨了 hAM 作为生物材料的作用，详细介绍了其组成和特性，强调了评估超微结构成分的重要性，并建议将拉曼光谱作为实现这一目的的重要技术。
Evaluation methods for decellularized tissues: A focus on human amniotic membrane.
Tissue engineering, a multidisciplinary research field aiming to revolutionize regenerative medicine, relies on scaffolds for optimal cell cultures and organ development. Decellularized tissue extracellular matrices (dECM) scaffolds, particularly from human amniotic membrane (hAM), show promise in clinical applications. This review discusses the significance of scaffolds, emphasizing dECM-based hAM scaffolds, delving into ECM complexities, decellularization processes, and evaluation methods. Raman spectroscopy emerges as a non-destructive tool for evaluating ECM preservation, presenting potential for quantifying ECM components in hAM before and after decellularization. The review explores the role of hAM as a biomaterial, detailing its composition and characteristics and emphasizes the importance of evaluating ultrastructural components and suggests Raman spectroscopy as a valuable technique for this purpose.
期刊介绍：
The Journal of Bioscience and Bioengineering is a research journal publishing original full-length research papers, reviews, and Letters to the Editor. The Journal is devoted to the advancement and dissemination of knowledge concerning fermentation technology, biochemical engineering, food technology and microbiology.
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