Journal of bioscience and bioengineering最新文献_第4页

Enhanced heparosan biosynthesis in Escherichia coli Nissle 1917 through carbon flux redirection 通过碳通量重定向增强大肠杆菌Nissle 1917中肝磷脂的生物合成。

IF 2.9 4区生物学

Journal of bioscience and bioengineering Pub Date : 2025-08-05 DOI: 10.1016/j.jbiosc.2025.07.005

Fangqi Shao , Ruiji Wu , Zheng-Jun Li

引用次数: 0

Metabolites and nutritional variations of phenotypic diversity in Kopyor coconut (Cocos nucifera L. var. Kopyor) Kopyor椰子（Cocos nucifera L. var. Kopyor）代谢物及其表型多样性的营养变化

IF 2.9 4区生物学

Journal of bioscience and bioengineering Pub Date : 2025-08-05 DOI: 10.1016/j.jbiosc.2025.07.002

Mercy Bientri Yunindanova , Hengky Novarianto , Eiichiro Fukusaki , Sastia Prama Putri

{"title":"Metabolites and nutritional variations of phenotypic diversity in Kopyor coconut (Cocos nucifera L. var. Kopyor)","authors":"Mercy Bientri Yunindanova , Hengky Novarianto , Eiichiro Fukusaki , Sastia Prama Putri","doi":"10.1016/j.jbiosc.2025.07.002","DOIUrl":"10.1016/j.jbiosc.2025.07.002","url":null,"abstract":"<div><div>Kopyor (<em>Cocos nucifera</em> L. var. Kopyor) is a distinct coconut variant resulting from a genetic mutation that alters the endosperm. Kopyor displays notable phenotypic diversity in terms of cultivar and endosperm quantity (EQ), which refers to the amount of flesh detached from the endocarp. Despite its distinct traits, kopyor utilization is limited because of the lack of comprehensive research on its diverse phenotypes. The aim of this study was to investigate the variations of metabolites and nutrients in kopyors by analyzing metabolomic profiles, physicochemical properties, and proximate characteristics across three cultivars with EQ levels ranging from 10 % to 50 %. The results showed that EQ influenced metabolite accumulation more significantly than the cultivar did. Elevated levels of EQ have been associated with increased concentrations of essential amino acids, which are classified as sensory-related metabolites. An increase in EQ was accompanied by the breakdown of macromolecules, particularly carbohydrates and proteins into simpler sugars and amino acids. This process potentially enhanced the bioavailability of nutrients in kopyor coconuts compared to that in normal coconuts. This study proposed potential metabolite marker for assessing the quality of kopyor coconuts. Valine, an essential amino acid, was the only compound consistently and significantly detected across all analyses, including principal component analysis (PCA), F-test, Tukey’s test, Pearson correlation, and orthogonal projections to latent structures regression (OPLSR), conducted on the water and flesh of kopyor coconuts. Consequently, valine was identified as a promising biomarker candidate. This study is the first to extensively characterize the phenotypic diversity of kopyor. The findings highlight its potential for further development in the food industry.</div></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"140 4","pages":"Pages 218-227"},"PeriodicalIF":2.9,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144768681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Construction of a heterologous protein secretion system using the protein disulfide isomerase Pdi1p in Schizosaccharomyces pombe 利用裂糖菌体蛋白二硫异构酶Pdi1p构建异源蛋白分泌系统。

IF 2.9 4区生物学

Journal of bioscience and bioengineering Pub Date : 2025-07-31 DOI: 10.1016/j.jbiosc.2025.07.003

Akihiro Tominaga , Tetsuya Kotani , Masahiro Watanabe , Kaoru Takegawa

{"title":"Construction of a heterologous protein secretion system using the protein disulfide isomerase Pdi1p in Schizosaccharomyces pombe","authors":"Akihiro Tominaga , Tetsuya Kotani , Masahiro Watanabe , Kaoru Takegawa","doi":"10.1016/j.jbiosc.2025.07.003","DOIUrl":"10.1016/j.jbiosc.2025.07.003","url":null,"abstract":"<div><div>The fission yeast <em>Schizosaccharomyces pombe</em> has been often used as a host for heterologous protein production; however, a method for extracellular secretion of heterologous protein would be advantageous for ease of purification and for native protein structure. In a previous study, overexpression of endogenous protein disulfide isomerase (PDI) genes improved the secretion of recombinant human transferrin in <em>S. pombe</em>. In the present study, we have explored whether Pdi1 can be used for the secretion of heterologous proteins in <em>S. pombe.</em> Overexpression of a fusion protein of Pdi1p and the heterologous protein EGFP (Pdi1p-EGFP), in the host <em>S. pombe</em> A8 strain, which lacks eight intracellular and extracellular proteases, resulted in efficient extracellular secretion of the fusion protein. To identify the optimal region of Pdi1p for use as an extracellular carrier, we compared the secretion of EGFP fused to the N-terminal Pdi1p signal domain and deletion mutants of Pdi1p. The signal sequence alone did not improve secretion, but deletion of two domains at the C-terminus did improve secretion. Notably, the x domain was important for secretion of the fusion protein. As a result of these findings, we have established a system for efficient secretion of target heterologous proteins by using optimally designed Pdi1p as a carrier for extracellular secretion.</div></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"140 4","pages":"Pages 237-243"},"PeriodicalIF":2.9,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144764951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Smell of stress: An in-depth look into the Bacillus subtilis 168 volatilome during pH stress using secondary electrospray ionization-Orbitrap mass spectrometry 胁迫的气味：利用二次电喷雾电离-轨道rap质谱法深入研究pH胁迫下枯草芽孢杆菌168挥发物。

IF 2.9 4区生物学

Journal of bioscience and bioengineering Pub Date : 2025-07-31 DOI: 10.1016/j.jbiosc.2025.05.008

Hendrik G. Mengers , Frederik Völker , Lars M. Blank

{"title":"Smell of stress: An in-depth look into the Bacillus subtilis 168 volatilome during pH stress using secondary electrospray ionization-Orbitrap mass spectrometry","authors":"Hendrik G. Mengers , Frederik Völker , Lars M. Blank","doi":"10.1016/j.jbiosc.2025.05.008","DOIUrl":"10.1016/j.jbiosc.2025.05.008","url":null,"abstract":"<div><div><em>Bacillus subtilis</em> is one of the best-studied organisms and of high importance in biotechnology. Depending on the application area, the bacterium encounters pH perturbations, potentially influencing growth and production rates. While the general stress response of <em>B. subtilis</em> is well studied, much is yet to be explored about its acid stress response. Using novel secondary electrospray ionization (SESI)-Orbitrap mass spectrometry technology, fermentation off-gas during acid stress applied to resting cells was measured online, in real time with a time resolution of 0.3 Hz. In total over 450 biogenic compounds were measured over 16,000 times after acidification. While some compounds were visible directly after acid addition, some increased in intensity over 2 h after the stress was induced. The most intense compounds measured in this condition were acetoin, a well-known <em>Bacillus</em> volatile, and C<sub>4</sub>H<sub>6</sub>O, which was not described before. This study provides another piece to the puzzle of the <em>B. subtilis</em> acid stress response by analysing the often-overlooked volatilome using a novel analytical method.</div></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"140 4","pages":"Pages 252-259"},"PeriodicalIF":2.9,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144764953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to “Effect of extraction methods on the preparation of electrospun/electrosprayed microstructures of tilapia skin collagen” [J. Biosci. Bioeng. 128 (2) (2019) 234–240] “提取方法对制备罗非鱼皮肤胶原蛋白静电纺丝/静电喷涂微结构的影响”的更正[J]。Biosci。生物工程学报，32(2)(2019):234-240。

IF 2.9 4区生物学

Journal of bioscience and bioengineering Pub Date : 2025-07-31 DOI: 10.1016/j.jbiosc.2025.07.001

Chunhuan Bi , Xiaohui Li , Qi Xin , Wei Han , Cuiping Shi , Ruihua Guo , Wenzheng Shi , Ruirui Qiao , Xichang Wang , Jian Zhong

引用次数: 0

Application of algae-bacteria symbiosis system for ammonia nitrogen wastewater treatment 藻类-细菌共生系统在氨氮废水处理中的应用。

IF 2.9 4区生物学

Journal of bioscience and bioengineering Pub Date : 2025-07-29 DOI: 10.1016/j.jbiosc.2025.06.011

Chun-Yen Chen , Yu-Han Chang , Yoong Kit Leong , Jo-Shu Chang

{"title":"Application of algae-bacteria symbiosis system for ammonia nitrogen wastewater treatment","authors":"Chun-Yen Chen , Yu-Han Chang , Yoong Kit Leong , Jo-Shu Chang","doi":"10.1016/j.jbiosc.2025.06.011","DOIUrl":"10.1016/j.jbiosc.2025.06.011","url":null,"abstract":"<div><div>In industrial processes, the primary sources of ammonia nitrogen emissions are organic matter and nitrogen-containing chemicals. When released directly into the environment, these nitrogen compounds elevate aquatic toxicity and reduce dissolved oxygen levels, significantly affecting aquatic ecosystems. This review introduces both traditional and novel ammonia nitrogen wastewater treatment technologies. Traditional methods include physical, chemical, and biological processes. The focus of this review is on novel ammonia nitrogen wastewater treatment technology based on algae-bacteria symbiosis systems. The review discusses key environmental factors influencing the algae-bacteria symbiosis system, such as temperature, light intensity, carbon dioxide concentration, and bioflocculation. Furthermore, it presents innovative large-scale algae-bacteria symbiosis system designed to achieve high carbon dioxide removal efficiency while effectively treating ammonia nitrogen wastewater with low energy consumption. This review aims to provide valuable insights that support the future development of efficient and commercially viable novel technologies for treating ammonia-nitrogen wastewater.</div></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"140 4","pages":"Pages 181-192"},"PeriodicalIF":2.9,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144742186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Construction of a simplified co-culture system using MDCK cells as surrogate intestinal epithelium with intestinal anaerobic bacterial mixtures 以MDCK细胞代替肠道上皮与肠道厌氧细菌混合物的简化共培养体系的构建。

IF 2.9 4区生物学

Journal of bioscience and bioengineering Pub Date : 2025-07-18 DOI: 10.1016/j.jbiosc.2025.06.010

Yoshihiro Umehara , Hideki Aoyagi

{"title":"Construction of a simplified co-culture system using MDCK cells as surrogate intestinal epithelium with intestinal anaerobic bacterial mixtures","authors":"Yoshihiro Umehara , Hideki Aoyagi","doi":"10.1016/j.jbiosc.2025.06.010","DOIUrl":"10.1016/j.jbiosc.2025.06.010","url":null,"abstract":"<div><div><em>In vitro</em> co-culture systems that mimic the intestinal environment are important tools for analysing interactions among intestinal bacterial communities. Conventional systems face challenges in co-culturing microorganisms with various oxygen requirements and aerobic intestinal epithelial cells, particularly in distinguishing and quantifying multiple bacterial species. To address these challenges, as a first step, we established a simplified co-culture system using a partially oil-sealed co-culture system (POS-CCS). Using <em>Lactobacillus paragasseri</em> and <em>Bifidobacterium longum</em> subsp. <em>longum</em> as model microorganisms, we successfully co-cultured them with MDCK cells and analysed their growth using multiplex quantitative polymerase chain reaction targeting the V6 region of the 16S rRNA gene. Our system allowed the individual measurement of growth curves of both bacterial species, and changes in the transepithelial electrical resistance of epithelial cells, cell viability, pH, and concentration of bacterial cells during co-culture. Notably, co-culture with MDCK cells led to a rapid decrease in pH, which coincided with an increase in transepithelial electrical resistance, suggesting a potential link between bacterial metabolism and epithelial barrier function. Furthermore, MDCK cells promoted the growth of both <em>L. paragasseri</em> and <em>B. longum</em> subsp. <em>longum</em>. This simplified and adaptable co-culture system offers a valuable tool for investigating host–microbe interactions in the gut and is expected to contribute to studies on probiotics, prebiotics, and control of the intestinal environment.</div></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"140 4","pages":"Pages 228-236"},"PeriodicalIF":2.9,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144667719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Solid-state cultivation of Aspergillus oryzae using insoluble plant cell wall polysaccharides and expression analyses of plant polysaccharide degradation-related enzymes 利用不溶性植物细胞壁多糖固态培养米曲霉及植物多糖降解相关酶的表达分析。

IF 2.9 4区生物学

Journal of bioscience and bioengineering Pub Date : 2025-07-15 DOI: 10.1016/j.jbiosc.2025.06.013

Tomohiko Matsuzawa , Masakazu Ishikawa , Shimma Fujiwa , Naoki Shimada , Hiroshi Kanzaki

{"title":"Solid-state cultivation of Aspergillus oryzae using insoluble plant cell wall polysaccharides and expression analyses of plant polysaccharide degradation-related enzymes","authors":"Tomohiko Matsuzawa , Masakazu Ishikawa , Shimma Fujiwa , Naoki Shimada , Hiroshi Kanzaki","doi":"10.1016/j.jbiosc.2025.06.013","DOIUrl":"10.1016/j.jbiosc.2025.06.013","url":null,"abstract":"<div><div><em>Aspergillus oryzae</em> produces multiple plant polysaccharide degradation-related enzymes, which make an important contribution to brewing and biotechnology. In this study, to clarify the mechanisms underlying the enzymatic degradation of plant cell wall polysaccharides by <em>A. oryzae</em>, we prepared insoluble plant cell wall polysaccharides from two plants, mung bean hypocotyls and Chinese chives, and used these as substrates for solid-state cultivation of this fungus. <em>A. oryzae</em> secretes numerous types of carbohydrate-active enzymes that are involved in the degradation of cellulosic and hemicellulosic polysaccharides among insoluble plant cell wall polysaccharides. Transcriptomic analyses revealed that genes encoding both characterized and uncharacterized carbohydrate-active enzymes were highly expressed in solid-state cultures using insoluble plant cell wall polysaccharides, indicating that these enzymes cooperatively degrade plant cell wall polysaccharides.</div></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"140 4","pages":"Pages 200-210"},"PeriodicalIF":2.9,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144649582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Early-stage transplant responses in miniature-liver implants based on decellularization-recellularization technology 基于脱细胞-再细胞化技术的微型肝植入物早期移植反应。

IF 2.9 4区生物学

Journal of bioscience and bioengineering Pub Date : 2025-07-11 DOI: 10.1016/j.jbiosc.2025.06.005

Lucija Stefan , Mario Kokichi Uehara , Yasuhiro Ikegami , Nana Shirakigawa , Yusuke Sakai , Hiroshi Mizumoto , Shinichi Aishima , Yo-ichi Yamashita , Hiroyuki Ijima

{"title":"Early-stage transplant responses in miniature-liver implants based on decellularization-recellularization technology","authors":"Lucija Stefan , Mario Kokichi Uehara , Yasuhiro Ikegami , Nana Shirakigawa , Yusuke Sakai , Hiroshi Mizumoto , Shinichi Aishima , Yo-ichi Yamashita , Hiroyuki Ijima","doi":"10.1016/j.jbiosc.2025.06.005","DOIUrl":"10.1016/j.jbiosc.2025.06.005","url":null,"abstract":"<div><div><span><span>Decellularization and recellularization can help address the gap between organ demand and availability. Although several whole organ engineered liver constructs have been reported, they are unable to maintain long-term function. Additionally, the effect of the early stage on cells after implantation is not described in detail. To better understand which factors affect initial </span>cell viability<span>, we inoculated primary hepatocytes into a miniature recellularized liver (RCL). In this study, RCL grafts<span> based on the decellularized right lobe of a mouse were populated with 1 × 10</span></span></span><sup>7</sup><span><span> primary hepatocytes of rat (PRH) origin. The scaffolds exhibited spatially uniform decellularization and good collagen retention in vitro. Once repopulated and implanted into the mesentery<span>, hepatocyte-specific function was confirmed in PRH grafts (Alb, 40 %; Hnf4a, 150 %) after 24 h, with </span></span>apoptosis<span> (Casp3) and cell–cell interaction (Tjp1) markers comparable to freshly isolated cells. Despite these positive results, we observed hypoxic conditions in the graft based on Hif1a expression, which leave room for improvement in the future. We believe that these findings will help future researchers understand the primary cell behavior of bioengineered liver constructs in vivo.</span></span></div></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"140 4","pages":"Pages 244-251"},"PeriodicalIF":2.9,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144618115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to “Early detection of leptospirosis using Anti-LipL32 carbon nanotube immunofluorescence probe” [J. Biosci. Bioeng. 130 (4) (2020) 424–430] “Anti-LipL32碳纳米管免疫荧光探针早期检测钩端螺旋体病”的勘误表[J]。Biosci。生物工程，130(4)(2020):424-430。

IF 2.9 4区生物学

Journal of bioscience and bioengineering Pub Date : 2025-07-10 DOI: 10.1016/j.jbiosc.2025.06.012

Kannan Sapna , Mohammed Tarique , Ashaiba Asiamma , Terikere Nagaraj Ravi Kumar , Vishwanath Shashidhar , Ananthapadmanabha Bhagwath Arun , Kariate Sudhakara Prasad

引用次数: 0