Journal of bioscience and bioengineering最新文献

{"title":"Positive impact of pyrocarbon and mechanical loading on cartilage-like tissue synthesis in a scaffold-free process","authors":"Imbert De Gaudemaris ,&nbsp;Amira Hannoun ,&nbsp;Rémy Gauthier ,&nbsp;Nina Attik ,&nbsp;Leyre Brizuela ,&nbsp;Saida Mebarek ,&nbsp;Michel Hassler ,&nbsp;Carole Bougault ,&nbsp;Ana-Maria Trunfio-Sfarghiu","doi":"10.1016/j.jbiosc.2024.09.005","DOIUrl":"10.1016/j.jbiosc.2024.09.005","url":null,"abstract":"<div><div>Aiming to build a tissue analogue engineered cartilage from differentiated chondrocytes, we investigated the potential of a pyrocarbon (PyC)-based and scaffold-free process, under mechanical stimulation. PyC biomaterial has shown promise in arthroplasty and implant strategies, and mechanical stimulation is recognized as an improvement in regeneration strategies. The objective was to maintain the cell phenotype to produce constructs with cartilage-like matrix composition and mechanical properties. Primary murine chondrocytes were deposited in drop form between two biomaterial surfaces expanded to 500 μm and a uniaxial cyclic compression was applied thanks to a handmade tribo-bioreactor (0.5 Hz, 100 μm of amplitude, 17 days). Histology and immunohistochemistry analysis showed that PyC biomaterial promoted expression of cartilage-like matrix components (glycosaminoglycans, type II collagen, aggrecan). Importantly, constructs obtained in dynamic conditions were denser and showed a cohesive and compact shape. The most promising condition was the combined use of PyC and dynamic stimulation, resulting in constructs of low elasticity and high viscosity, thus with an increased damping factor. We verified that no calcium deposits were detectable and that type X collagen was not expressed, suggesting that the cells had not undergone hypertrophic maturation. While most studies focus on the comparison of different biomaterials or on the effect of different mechanical stimuli separately, we demonstrated the value of combining the two approaches to get as close as possible to the biological and mechanical qualities of natural hyaline articular cartilage.</div></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"139 1","pages":"Pages 53-59"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142466308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular mechanisms of complex-type N-glycan breakdown and metabolism by the human intestinal bacterium Barnesiella intestinihominis","authors":"Kanako Doi,&nbsp;Kazuki Mori,&nbsp;Misaki Komatsu,&nbsp;Akari Shinoda,&nbsp;Kosuke Tashiro,&nbsp;Yujiro Higuchi,&nbsp;Jiro Nakayama,&nbsp;Kaoru Takegawa","doi":"10.1016/j.jbiosc.2024.10.006","DOIUrl":"10.1016/j.jbiosc.2024.10.006","url":null,"abstract":"<div><div>Intestinal bacteria play a crucial role in human health, for example, by maintaining immune and metabolic homeostasis and protecting against pathogens. Survival in the human intestine depends on the bacterium's ability to utilize complex carbohydrates. Some species are known to use host-derived glycans; for example, <em>Bifidobacteria</em> can utilize <em>O</em>-glycan of mucin. However, there are few studies on intestinal bacteria utilizing host-derived <em>N</em>-glycan. Here, we identified the mechanism underlying the breakdown and utilization of complex-type <em>N</em>-glycan by the human intestinal bacterium <em>Barnesiella intestinihominis</em>. A growth assay showed that <em>B. intestinihominis</em> can utilize complex-type <em>N</em>-glycan as a carbon source, while RNA-seq analysis identified enzymes and transporters involved in the mechanism of <em>N</em>-glycan breakdown. In particular, the expression of three genes encoding glycoside hydrolase 85 endo-β<em>-N</em>-acetylglucosaminidase (<em>endo-BIN1</em>, <em>endo-BIN2</em>, and <em>endo-BIN3</em>) rose markedly in bacterial cells cultured in complex-type N-glycoprotein medium. We also found that the <em>susC</em> and <em>susD</em> genes, encoding the SusC/SusD membrane complex, form a gene cluster with <em>endo-BIN</em> genes, suggesting that SusC/SusD is involved in transportation of the glycan into the cell. Other genes encoding exo-type glycoside hydrolase enzymes showed elevated expression in cells grown in complex-type N-glycoprotein medium, suggesting that these enzymes function in further degradation of glycan for metabolism by the bacterium. Collectively, these findings suggest the survival strategy of an intestinal bacterium that has a unique metabolic pathway to use host-derived complex-type <em>N</em>-glycan as a nutrient.</div></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"139 1","pages":"Pages 14-22"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142604889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of induced pluripotent stem cell differentiation into neural progenitor cell using Raman spectra derived from extracellular vesicles in culture supernatants","authors":"Kakuro Hirai ,&nbsp;Hikaru Saito ,&nbsp;Midori Kato ,&nbsp;Masaharu Kiyama ,&nbsp;Hiroko Hanzawa ,&nbsp;Atsushi Nakane ,&nbsp;Sayaka Sekiya ,&nbsp;Kenji Yoshida ,&nbsp;Akiyoshi Kishino ,&nbsp;Atsushi Ikeda ,&nbsp;Toru Kimura ,&nbsp;Jun Takahashi ,&nbsp;Shizu Takeda","doi":"10.1016/j.jbiosc.2024.09.004","DOIUrl":"10.1016/j.jbiosc.2024.09.004","url":null,"abstract":"<div><div>Non-invasive cell culture monitoring technology is crucial to improve the manufacturing efficiency of cell products. We have found that extracellular vesicles (EVs) are secreted into the culture supernatants in the differentiation process from human induced pluripotent stem cells (iPSCs) to dopaminergic progenitor cells, and that the composition of EVs changes in accordance with the differentiation processes. In this study, we hypothesized that it is possible to evaluate the cultured cellular states by detecting compositional changes of EVs secreted from cultured cells with label-free Raman spectroscopy in a non-invasive manner. Therefore, Raman signal analysis derived from EV fractions isolated from culture supernatants throughout the differentiation process was conducted. iPSCs cultures were simultaneously implemented under a standard condition (control) and an artificial deviation condition inducing reductions in pluripotency by depleting FGF2 in culture medium (-FGF2), which is indispensable for maintaining the pluripotency. Subsequently, the differentiation step was conducted for each iPSCs culture under the same condition. As a result, it was found that under -FGF2, the expression level of the pluripotency marker <em>NANOG</em> decreased compared to that of the control and correlated with the identification results based on Raman signals with a correlation coefficient of 0.77. Lipid-derived Raman signals were extracted as identification factors, suggesting that changes in the lipid component of EV occur depending on the cellular states. From the above, we have found that the change in composition of EVs in the culture supernatant by detecting Raman signals would be a monitoring index of the cellular state of differentiation and pluripotency.</div></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"139 1","pages":"Pages 44-52"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142466297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mass spectrometry imaging of gamma-aminobutyric acid and glutamic acid decarboxylase reactions at various stages of banana ripening","authors":"Shiho Ishimoto ,&nbsp;Eiichiro Fukusaki ,&nbsp;Shuichi Shimma","doi":"10.1016/j.jbiosc.2024.10.001","DOIUrl":"10.1016/j.jbiosc.2024.10.001","url":null,"abstract":"<div><div>Banana is the fourth most consumed crop worldwide, and its high economic value and health benefits have made it very popular. Bananas are climacteric fruits that ripen after harvesting. It has been reported that the endogenous substances in bananas change significantly during the ripening process. This study focused on levels of gamma-aminobutyric acid (GABA) and glutamic acid decarboxylase (GAD), an enzyme that catalyzes the synthesis of GABA, which reportedly fluctuates during the ripening stage. Previous studies have shown that GAD expression is associated with banana ripening; however, changes in its distribution during ripening have not been verified. This study aimed to clarify the relationship between GABA and GAD during ripening of ethylene-treated bananas. Visualization of the localization of endogenous GABA and GAD was performed using mass spectrometry imaging. To visualize GAD reaction, a glutamate-d₃ (labeled substrate) was supplied to the sample, and a GABA-d₃ (labeled product) was regarded as the localization of the enzymatic reaction. Liquid chromatography-mass spectrometry was also used to confirm the amount of GABA and activity of the GAD. This will allow us to clarify the direct relationship between GABA and GAD and to understand the role of the GAD reaction in phytohormones.</div></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"139 1","pages":"Pages 79-84"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142557905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plasma-activated medium suppresses proliferation and migration of human lung cancer cells by regulating PI3K/AKT-Wnt signaling pathway","authors":"Zhidan Sun ,&nbsp;Chenglong Ding ,&nbsp;Yuhan Wang ,&nbsp;Han Zhou ,&nbsp;Wencheng Song","doi":"10.1016/j.jbiosc.2024.10.002","DOIUrl":"10.1016/j.jbiosc.2024.10.002","url":null,"abstract":"<div><div>The main causes of high mortality in lung cancer patients are the malignant growth and migration of cancer cells. This study aims to investigate the underlying mechanisms of low-temperature plasma-activated medium (PAM) treating human lung cancer (HLC). Changes in the levels of reactive oxygen and nitrogen species both inside and outside the cells were evaluated. Our results showed that prolonged PAM exposure decreased cell viability, raised intracellular reactive oxygen species levels, and hindered cell migration while reducing mitochondrial membrane potential. Protein analysis revealed PAM increased GSK-3β and p-β-catenin expression but decreased PI3K, AKT, p-AKT, p-GSK-3β, Wnt, and β-catenin levels, thereby inhibiting the epithelial–mesenchymal transition. These findings suggest PAM suppresses HLC cells proliferation and migration by blocking the PI3K/AKT-Wnt pathway. The study will provide a valuable theoretical basis for future low-temperature plasma treatment, thereby improving the survival rates and prognosis of lung cancer.</div></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"139 1","pages":"Pages 60-69"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142621139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of a low acetate-producing strain of Tetragenococcus halophilus to soy sauce fermentation","authors":"Keita Higuchi ,&nbsp;Yuya Nukagawa ,&nbsp;Takura Wakinaka ,&nbsp;Jun Watanabe ,&nbsp;Yoshinobu Mogi","doi":"10.1016/j.jbiosc.2024.09.007","DOIUrl":"10.1016/j.jbiosc.2024.09.007","url":null,"abstract":"<div><div>In soy sauce brewing, the halophilic lactic acid bacterium, <em>Tetragenococcus halophilus</em> is used as a fermentation starter and contributes to the taste and aroma of soy sauce, mainly by producing lactate. By lowering the pH of the soy sauce mash, lactate serves as a suitable growth environment for the halotolerant yeast <em>Zygosaccharomyces rouxii</em>. Acetate, which is produced by <em>T. halophilus</em> via the citrate metabolic pathway, is a critical growth inhibitory factor for <em>Z. rouxii</em>. Therefore, a <em>T. halophilus</em> strain that lacks acetate production could be an ideal fermentation starter to enhance ethanol production. In this study, we obtained a derivative of <em>T. halophilus</em> containing an insertion sequence in <em>citC</em>, which is an essential gene for citrate metabolism, and validated its performance as a soy sauce fermentation starter. The derivative neither metabolized citrate nor produced excessive acetate in soy sauce mash, resulting in vigorous alcohol fermentation by <em>Z. rouxii</em>. This study provides insights into the application of a low acetate-producing strain of <em>T. halophilus</em> as a starter to produce soy sauce with high alcohol content and low sour aroma.</div></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"139 1","pages":"Pages 23-29"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142466295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Amphiphilic phospholipid polymers as a cryoprotectant for vitrification and nanowarming of rat livers","authors":"Masahiro Kaneko ,&nbsp;Natsumi Takizawa ,&nbsp;Taisei Wakabayashi ,&nbsp;Hidenori Kaneoka ,&nbsp;Akira Ito","doi":"10.1016/j.jbiosc.2024.10.003","DOIUrl":"10.1016/j.jbiosc.2024.10.003","url":null,"abstract":"<div><div>Liver biobanking is a promising approach that saves the lives of patients with end-stage liver disease. Cryopreservation based on vitrification enables semi-permanent organ preservation, contributing to overcome the shortage of donors for liver transplants. A technical challenge in cryopreservation of transplantable organs lies in thawing methodology, and conventional convective warming cannot maintain the glassy state during thawing because of the large temperature gradient between the inner and outer parts of the organs, leading to ice formation and damage of cells in the organ. Nanowarming, in which magnetic nanoparticles are dispersed in a vitrification solution and heated by exposure of alternating magnetic field, can achieve uniform and rapid heating of organs. Herein, we report that amphiphilic phospholipid polymers composed of 2-methacryloyloxyethyl phosphorylcholine and <em>n</em>-butyl methacrylate can function as a cryoprotectant for nanowarming. The amphiphilic phospholipid polymers enhanced the viability of primary rat hepatocytes after vitrification. Moreover, the polymers enhanced the dispersion stability of magnetic nanoparticles in vitrification solution, and the perfusion of the vitrification solution with magnetic nanoparticles into rat livers through portal vein provided uniform distribution of the nanoparticles in the liver. After perfusion, the vitrified liver was successfully thawed rapidly and uniformly by nanowarming, which maintained tissue integrity and cell viability.</div></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"139 1","pages":"Pages 70-78"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142501013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Geobacter sulfurreducens strain 60473, a potent bioaugmentation agent for improving the performances of bioelectrochemical systems","authors":"Tomoka Harada ,&nbsp;Yohei Yamada ,&nbsp;Mizuki Toda ,&nbsp;Yuki Takamatsu ,&nbsp;Keisuke Tomita ,&nbsp;Kengo Inoue ,&nbsp;Atsushi Kouzuma ,&nbsp;Kazuya Watanabe","doi":"10.1016/j.jbiosc.2024.10.007","DOIUrl":"10.1016/j.jbiosc.2024.10.007","url":null,"abstract":"<div><div>Bioaugmentation with electrochemically active bacteria (EAB) has been suggested useful for improving the performance of bioelectrochemical systems (BESs) for sustainable energy generation, while its success is dependent on EAB introduced into the systems. Here we report on the isolation of a novel EAB, <em>Geobacter sulfurreducens</em> strain 60473, from microbes that colonized on an anode of a sediment microbial fuel cell. This strain is highly adhesive to graphite electrodes, forms dense biofilms on electrode surfaces, and generates high current densities in BESs. When microbial electrolysis cells (MECs) inoculated with paddy-field soil and fed starch as the major organic substrate were augmented with strain 60473, <em>Geobacter</em> bacteria predominantly colonized on anodes, and MEC performances, including current generation, hydrogen production and organics removal, were substantially improved compared to non-bioaugmented controls. Results suggest that bioaugmentation with electrode-adhesive EAB, such as strain 60473, is a promising approach for improving the performance of BESs, including MECs treating fermentable organics and biomass wastes.</div></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"139 1","pages":"Pages 36-43"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142604875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and application to wastewater treatment of Pseudomonas sp. No. 44 possessing high formaldehyde tolerance","authors":"Eri Nasuno ,&nbsp;Minato Kodera ,&nbsp;Atsuya Seki ,&nbsp;Hidenori Kofune ,&nbsp;Norihiro Kato","doi":"10.1016/j.jbiosc.2024.12.002","DOIUrl":"10.1016/j.jbiosc.2024.12.002","url":null,"abstract":"<div><div>Bacteria and fungi that are resistant to formaldehyde (FA) are expected to use biochemical processing to degrade FA in wastewater. <em>Pseudomonas</em> sp. No. 44, which carries the FA dismutase and several FA dehydrogenase genes, was isolated from industrial wastewater as an FA-degrading strain that did not match the genome sequence of any previously reported FA-degrading bacteria. While maintaining a low cell density (OD₆₀₀ = 0.1), almost all the FA (1 g L⁻¹) was degraded within 6 h, after which cell proliferation predominated. Furthermore, increasing the cell density to OD₆₀₀ = 1.0 maintained high FA resistance and rapid degradation performance within 3 h, even at concentrations as high as 5 g L⁻¹. Formic acid, which temporarily increased in concentration as the FA decomposed, disappeared as the culture progressed, suggesting that this strain also exhibited high formate dehydrogenase activity. This practical performance exceeded that of any reported FA-degrading microorganism, including fungi. Vacuum-dried bacterial cells exhibited FA decomposition properties at the same level as those of live cells in the presence of phosphate due to the maintenance of enzymatic activity. The dried bacterial cells of strain No. 44, which are easy to handle and store, are expected to allow the maintenance of FA concentration in wastewater below the emission regulation concentration for each region through the addition of the strain at appropriate intervals.</div></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"139 3","pages":"Pages 182-186"},"PeriodicalIF":2.3,"publicationDate":"2024-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142907124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unfolding and refolding of GH19 chitinase Chi19MK with antifungal activity from Lysobacter sp. MK9-1 at low pH and high temperature","authors":"Moe Yokomichi,&nbsp;Haruki Kanno,&nbsp;Hiroyuki Konno,&nbsp;Koki Makabe,&nbsp;Shigekazu Yano","doi":"10.1016/j.jbiosc.2024.12.006","DOIUrl":"10.1016/j.jbiosc.2024.12.006","url":null,"abstract":"<div><div>The GH19 chitinase Chi19MK from <em>Lysobacter</em> sp. MK9-1 inhibits fungal growth. In this study, the thermal stability of Chi19MK was investigated in buffers of different pH. Chi19MK retained approximately 100 % of its activity at 60 °C for 2 h in a sodium acetate buffer at pH 4.0. However, the enzyme retained approximately 15 % of its activity at 60 °C in potassium phosphate buffer (pH 6.0) and acetate (pH 5.0). Chi19MK was treated with acetate buffer (pH 4.0) for 1–5 h at a temperature range of 60 °C–100 °C, Chi19MK retained more than 70 % of its activity after heat treatment at 60 °C, 70 °C, and 80 °C for 5 h, and its activity was reduced to approximately 65 % after treatment at 90 °C for 2 h and at 100 °C for 1 h. The circular dichroism (CD) spectrum of Chi19MK in 10 mM acetate buffer (pH 4.0) was recorded at 80 °C and the CD spectrum showed the collapse of secondary structures. After decreasing the solution temperature from 80 °C to 25 °C, the spectrum was almost restored to the initial state. In contrast, the secondary structures of Chi19MK were not restored in acetate buffer (pH 5.0) or phosphate buffer (pH 6.0) by cooling after heat treatment at 80 °C. In an antifungal assay against <em>Trichoderma reesei</em>, Chi19MK retained its growth inhibition activity after heat treatment at 70 °C in acetate buffer at pH 4.0. These findings suggest that Chi19MK refolds in acetate buffer at pH 4.0, even when its conformation is disrupted by exposure to high-temperature conditions.</div></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"139 3","pages":"Pages 165-171"},"PeriodicalIF":2.3,"publicationDate":"2024-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142907128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}