Journal of bioscience and bioengineering最新文献_第6页

Amphiphilic phospholipid polymers as a cryoprotectant for vitrification and nanowarming of rat livers 两亲磷脂聚合物作为玻璃化和纳米加热大鼠肝脏的低温保护剂

IF 2.3 4区生物学

Journal of bioscience and bioengineering Pub Date : 2025-01-01 DOI: 10.1016/j.jbiosc.2024.10.003

Masahiro Kaneko , Natsumi Takizawa , Taisei Wakabayashi , Hidenori Kaneoka , Akira Ito

{"title":"Amphiphilic phospholipid polymers as a cryoprotectant for vitrification and nanowarming of rat livers","authors":"Masahiro Kaneko , Natsumi Takizawa , Taisei Wakabayashi , Hidenori Kaneoka , Akira Ito","doi":"10.1016/j.jbiosc.2024.10.003","DOIUrl":"10.1016/j.jbiosc.2024.10.003","url":null,"abstract":"<div><div>Liver biobanking is a promising approach that saves the lives of patients with end-stage liver disease. Cryopreservation based on vitrification enables semi-permanent organ preservation, contributing to overcome the shortage of donors for liver transplants. A technical challenge in cryopreservation of transplantable organs lies in thawing methodology, and conventional convective warming cannot maintain the glassy state during thawing because of the large temperature gradient between the inner and outer parts of the organs, leading to ice formation and damage of cells in the organ. Nanowarming, in which magnetic nanoparticles are dispersed in a vitrification solution and heated by exposure of alternating magnetic field, can achieve uniform and rapid heating of organs. Herein, we report that amphiphilic phospholipid polymers composed of 2-methacryloyloxyethyl phosphorylcholine and <em>n</em>-butyl methacrylate can function as a cryoprotectant for nanowarming. The amphiphilic phospholipid polymers enhanced the viability of primary rat hepatocytes after vitrification. Moreover, the polymers enhanced the dispersion stability of magnetic nanoparticles in vitrification solution, and the perfusion of the vitrification solution with magnetic nanoparticles into rat livers through portal vein provided uniform distribution of the nanoparticles in the liver. After perfusion, the vitrified liver was successfully thawed rapidly and uniformly by nanowarming, which maintained tissue integrity and cell viability.</div></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"139 1","pages":"Pages 70-78"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142501013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Geobacter sulfurreducens strain 60473, a potent bioaugmentation agent for improving the performances of bioelectrochemical systems Geobacter sulfurreducens 菌株 60473，一种用于提高生物电化学系统性能的强效生物增强剂。

IF 2.3 4区生物学

Journal of bioscience and bioengineering Pub Date : 2025-01-01 DOI: 10.1016/j.jbiosc.2024.10.007

Tomoka Harada , Yohei Yamada , Mizuki Toda , Yuki Takamatsu , Keisuke Tomita , Kengo Inoue , Atsushi Kouzuma , Kazuya Watanabe

{"title":"Geobacter sulfurreducens strain 60473, a potent bioaugmentation agent for improving the performances of bioelectrochemical systems","authors":"Tomoka Harada , Yohei Yamada , Mizuki Toda , Yuki Takamatsu , Keisuke Tomita , Kengo Inoue , Atsushi Kouzuma , Kazuya Watanabe","doi":"10.1016/j.jbiosc.2024.10.007","DOIUrl":"10.1016/j.jbiosc.2024.10.007","url":null,"abstract":"<div><div>Bioaugmentation with electrochemically active bacteria (EAB) has been suggested useful for improving the performance of bioelectrochemical systems (BESs) for sustainable energy generation, while its success is dependent on EAB introduced into the systems. Here we report on the isolation of a novel EAB, <em>Geobacter sulfurreducens</em> strain 60473, from microbes that colonized on an anode of a sediment microbial fuel cell. This strain is highly adhesive to graphite electrodes, forms dense biofilms on electrode surfaces, and generates high current densities in BESs. When microbial electrolysis cells (MECs) inoculated with paddy-field soil and fed starch as the major organic substrate were augmented with strain 60473, <em>Geobacter</em> bacteria predominantly colonized on anodes, and MEC performances, including current generation, hydrogen production and organics removal, were substantially improved compared to non-bioaugmented controls. Results suggest that bioaugmentation with electrode-adhesive EAB, such as strain 60473, is a promising approach for improving the performance of BESs, including MECs treating fermentable organics and biomass wastes.</div></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"139 1","pages":"Pages 36-43"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142604875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation and application to wastewater treatment of Pseudomonas sp. No. 44 possessing high formaldehyde tolerance 具有高甲醛耐受性的 44 号假单胞菌的分离及在废水处理中的应用。

IF 2.3 4区生物学

Journal of bioscience and bioengineering Pub Date : 2024-12-30 DOI: 10.1016/j.jbiosc.2024.12.002

Eri Nasuno , Minato Kodera , Atsuya Seki , Hidenori Kofune , Norihiro Kato

{"title":"Isolation and application to wastewater treatment of Pseudomonas sp. No. 44 possessing high formaldehyde tolerance","authors":"Eri Nasuno , Minato Kodera , Atsuya Seki , Hidenori Kofune , Norihiro Kato","doi":"10.1016/j.jbiosc.2024.12.002","DOIUrl":"10.1016/j.jbiosc.2024.12.002","url":null,"abstract":"<div><div>Bacteria and fungi that are resistant to formaldehyde (FA) are expected to use biochemical processing to degrade FA in wastewater. <em>Pseudomonas</em> sp. No. 44, which carries the FA dismutase and several FA dehydrogenase genes, was isolated from industrial wastewater as an FA-degrading strain that did not match the genome sequence of any previously reported FA-degrading bacteria. While maintaining a low cell density (OD<sub>600</sub> = 0.1), almost all the FA (1 g L<sup>−1</sup>) was degraded within 6 h, after which cell proliferation predominated. Furthermore, increasing the cell density to OD<sub>600</sub> = 1.0 maintained high FA resistance and rapid degradation performance within 3 h, even at concentrations as high as 5 g L<sup>−1</sup>. Formic acid, which temporarily increased in concentration as the FA decomposed, disappeared as the culture progressed, suggesting that this strain also exhibited high formate dehydrogenase activity. This practical performance exceeded that of any reported FA-degrading microorganism, including fungi. Vacuum-dried bacterial cells exhibited FA decomposition properties at the same level as those of live cells in the presence of phosphate due to the maintenance of enzymatic activity. The dried bacterial cells of strain No. 44, which are easy to handle and store, are expected to allow the maintenance of FA concentration in wastewater below the emission regulation concentration for each region through the addition of the strain at appropriate intervals.</div></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"139 3","pages":"Pages 182-186"},"PeriodicalIF":2.3,"publicationDate":"2024-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142907124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unfolding and refolding of GH19 chitinase Chi19MK with antifungal activity from Lysobacter sp. MK9-1 at low pH and high temperature 具有抗真菌活性的 GH19 几丁质酶 Chi19MK 在低 pH 值和高温条件下的折叠和再折叠。

IF 2.3 4区生物学

Journal of bioscience and bioengineering Pub Date : 2024-12-30 DOI: 10.1016/j.jbiosc.2024.12.006

Moe Yokomichi, Haruki Kanno, Hiroyuki Konno, Koki Makabe, Shigekazu Yano

{"title":"Unfolding and refolding of GH19 chitinase Chi19MK with antifungal activity from Lysobacter sp. MK9-1 at low pH and high temperature","authors":"Moe Yokomichi, Haruki Kanno, Hiroyuki Konno, Koki Makabe, Shigekazu Yano","doi":"10.1016/j.jbiosc.2024.12.006","DOIUrl":"10.1016/j.jbiosc.2024.12.006","url":null,"abstract":"<div><div>The GH19 chitinase Chi19MK from <em>Lysobacter</em> sp. MK9-1 inhibits fungal growth. In this study, the thermal stability of Chi19MK was investigated in buffers of different pH. Chi19MK retained approximately 100 % of its activity at 60 °C for 2 h in a sodium acetate buffer at pH 4.0. However, the enzyme retained approximately 15 % of its activity at 60 °C in potassium phosphate buffer (pH 6.0) and acetate (pH 5.0). Chi19MK was treated with acetate buffer (pH 4.0) for 1–5 h at a temperature range of 60 °C–100 °C, Chi19MK retained more than 70 % of its activity after heat treatment at 60 °C, 70 °C, and 80 °C for 5 h, and its activity was reduced to approximately 65 % after treatment at 90 °C for 2 h and at 100 °C for 1 h. The circular dichroism (CD) spectrum of Chi19MK in 10 mM acetate buffer (pH 4.0) was recorded at 80 °C and the CD spectrum showed the collapse of secondary structures. After decreasing the solution temperature from 80 °C to 25 °C, the spectrum was almost restored to the initial state. In contrast, the secondary structures of Chi19MK were not restored in acetate buffer (pH 5.0) or phosphate buffer (pH 6.0) by cooling after heat treatment at 80 °C. In an antifungal assay against <em>Trichoderma reesei</em>, Chi19MK retained its growth inhibition activity after heat treatment at 70 °C in acetate buffer at pH 4.0. These findings suggest that Chi19MK refolds in acetate buffer at pH 4.0, even when its conformation is disrupted by exposure to high-temperature conditions.</div></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"139 3","pages":"Pages 165-171"},"PeriodicalIF":2.3,"publicationDate":"2024-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142907128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a unique tissue-engineered in vitro vascular model with endothelial layer-inverted vascular tissue structure using a cell self-aggregation technique 利用细胞自聚集技术建立具有内皮层-倒维管组织结构的独特组织工程体外血管模型。

IF 2.3 4区生物学

Journal of bioscience and bioengineering Pub Date : 2024-12-26 DOI: 10.1016/j.jbiosc.2024.12.001

Shingo Hashimoto , Akihiko Sugiyama , Tomoyuki Ota , Hiroshi Matsumoto , Yoshihiro Kimata , Ryosuke Iwai

{"title":"Development of a unique tissue-engineered in vitro vascular model with endothelial layer-inverted vascular tissue structure using a cell self-aggregation technique","authors":"Shingo Hashimoto , Akihiko Sugiyama , Tomoyuki Ota , Hiroshi Matsumoto , Yoshihiro Kimata , Ryosuke Iwai","doi":"10.1016/j.jbiosc.2024.12.001","DOIUrl":"10.1016/j.jbiosc.2024.12.001","url":null,"abstract":"<div><div>Vascular-like tissues composed of cells maintaining their shape and structure at any position in a culture dish without the use of gels or other artificial materials are ideal vascular models to test the effects of candidate drugs on cells without adsorption by artificial materials and analysis of structural changes over time. In this study, we aimed to prepare fiber-shaped cell aggregates composed of human umbilical vein endothelial and mesenchymal stem cells as vascular pericytes anchored to the bottom of culture dishes at a defined location using our developed cell self-aggregation technique and dumbbell-shaped culture groove. The fiber-shaped cell aggregates maintained their shape for at least two weeks without rupture, and histological analysis revealed that they formed a unique tissue structure with a gapless endothelial layer on the outer surface and capillary-like structures oriented in the same direction as the long axis of the fiber in the medial side. Moreover, exposure to cadmium chloride, a vascular toxicant, elicited toxic responses in vascular endothelialized fiber-shaped tissues, with only their outer endothelial layer being disintegrated but their fiber shape remaining intact. Overall, our results suggest the developed vascular endothelialized fiber-shaped tissue construct as potential models for vascular toxicity testing, facilitating the evaluation of toxicity over time without any effects of gel adsorption.</div></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"139 3","pages":"Pages 226-233"},"PeriodicalIF":2.3,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142894764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacterial removal using liposomes and an anionic adsorber 利用脂质体和阴离子吸附剂去除细菌。

IF 2.3 4区生物学

Journal of bioscience and bioengineering Pub Date : 2024-12-26 DOI: 10.1016/j.jbiosc.2024.11.002

Yohei Noda , Tomohiro Noguchi , Takashi Nagano , Wataru Aoki , Mitsuyoshi Ueda

{"title":"Bacterial removal using liposomes and an anionic adsorber","authors":"Yohei Noda , Tomohiro Noguchi , Takashi Nagano , Wataru Aoki , Mitsuyoshi Ueda","doi":"10.1016/j.jbiosc.2024.11.002","DOIUrl":"10.1016/j.jbiosc.2024.11.002","url":null,"abstract":"<div><div>Extracorporeal blood purification techniques using magnetic beads, which physically remove bacteria, fungi, viruses, and cytokines (disease agents) from the blood causing sepsis, have been studied. However, magnetic bead influx, which causes hemolysis and cytotoxicity, is an important issue. This study proposed a novel method for removing <em>Escherichia coli</em> from the blood using liposomes with high biocompatibility. To realize this method, a pegylated cationic liposome conjugated with antibodies (PCLA) that can simultaneously adsorb disease agents with the conjugated liposome antibodies and adhere to electrostatic absorbers was developed. <em>E. coli</em> was successfully adsorbed by PCLA in phosphate-buffered saline and electrostatically removed with a high removal efficiency of the antigen–antibody reaction (approximately 100 %). The removal efficiency of the antigen–antibody reaction in filtered bovine blood was approximately 50 %, demonstrating <em>E. coli</em> removal in the blood using the same method. Results suggested that this method can remove various disease agents from the blood by changing the antibody type.</div></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"139 3","pages":"Pages 249-256"},"PeriodicalIF":2.3,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142894727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolic analysis reveals the contribution of mechanosensitive channel MscM to extracellular release of glutamate in glycogen-deficient Synechococcus elongatus 代谢分析揭示了机械敏感通道MscM对糖原缺陷长聚球菌细胞外谷氨酸释放的贡献。

IF 2.3 4区生物学

Journal of bioscience and bioengineering Pub Date : 2024-12-20 DOI: 10.1016/j.jbiosc.2024.12.003

Yuichi Kato , Kouhei Kamasaka , Mami Matsuda , Hiroko Koizumi , Ryudo Ohbayashi , Hiroki Ashida , Akihiko Kondo , Tomohisa Hasunuma

{"title":"Metabolic analysis reveals the contribution of mechanosensitive channel MscM to extracellular release of glutamate in glycogen-deficient Synechococcus elongatus","authors":"Yuichi Kato , Kouhei Kamasaka , Mami Matsuda , Hiroko Koizumi , Ryudo Ohbayashi , Hiroki Ashida , Akihiko Kondo , Tomohisa Hasunuma","doi":"10.1016/j.jbiosc.2024.12.003","DOIUrl":"10.1016/j.jbiosc.2024.12.003","url":null,"abstract":"<div><div>In bacteria, mechanosensitive channels mediate extracellular release of osmolytes, including glutamate, functioning as safety valves upon osmotic downshift. In cyanobacteria, the role of mechanosensitive channels has not been completely elucidated. Recently, the glycogen-deficient Δ<em>glgC</em> mutant of <em>Synechococcus elongatus</em> PCC 7942 was found to release glutamate extracellularly, giving rise to a hypothesis that the role of mechanosensitive channels in cyanobacteria is conserved. Using the Δ<em>glgC</em> mutant as the model, the present study aimed to examine whether the putative mechanosensitive channel protein MscM mediates the extracellular release of glutamate. Compared to the Δ<em>glgC</em> mutant, the Δ<em>glgC</em> Δ<em>mscM</em> mutant was found to release less glutamate and aspartate extracellularly. In addition, intracellular levels of these amino acids were significantly higher in the Δ<em>glgC</em> Δ<em>mscM</em> mutant than in the Δ<em>glgC</em> mutant. These results suggested that MscM mediates the extracellular release of glutamate and aspartate in glycogen-deficient cyanobacteria. Furthermore, the Δ<em>glgC</em> Δ<em>mscM</em> mutant exhibited more elongated cell shapes compared to the wild type and Δ<em>glgC</em> single mutant, suggesting that the deletion of the <em>mscM</em> gene intensified turgor pressure and/or that MscM is involved in cell division. Through metabolic analysis, the present study revealed that mechanosensitive channel MscM in cyanobacteria is involved in the extracellular release of amino acids.</div></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"139 3","pages":"Pages 187-193"},"PeriodicalIF":2.3,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142872154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Specific hydroxylation and glucuronidation of 2′-hydroxyflavanone by Streptomyces coeruleorubidus NRRL B-2569 蓝纹链霉菌NRRL B-2569对2′-羟黄酮的羟基化和糖醛酸化作用。

IF 2.3 4区生物学

Journal of bioscience and bioengineering Pub Date : 2024-12-19 DOI: 10.1016/j.jbiosc.2024.11.004

Jie Ren, Kyle Jackson, Caleb Don Barton, Yu Huang, Jixun Zhan

{"title":"Specific hydroxylation and glucuronidation of 2′-hydroxyflavanone by Streptomyces coeruleorubidus NRRL B-2569","authors":"Jie Ren, Kyle Jackson, Caleb Don Barton, Yu Huang, Jixun Zhan","doi":"10.1016/j.jbiosc.2024.11.004","DOIUrl":"10.1016/j.jbiosc.2024.11.004","url":null,"abstract":"<div><div>Flavonoids constitute a class of natural compounds with varied bioactivities. Nevertheless, the potential health benefits of flavonoids for humans are often compromised by their low water solubility and limited bioavailability. In this study, four derivatives, namely 2′,5′-dihydroxyflavanone (<strong>2</strong>), 5′-dihydroxyflavone-2′-<em>O</em>-β-<span>d</span>-glucuronide (<strong>3</strong>), and two isomers of hydroxyflavanone-2′-<em>O</em>-β-<span>d</span>-glucuronide (<strong>4</strong> and <strong>5</strong>), were biosynthesized from substrate 2′-hydroxyflavanone (<strong>1</strong>) through the specific hydroxylation and glucuronidation using <em>Streptomyces coeruleorubidus</em> NRRL B-2569. Product <strong>2</strong> was identified as a known compound while products <strong>3</strong>–<strong>5</strong> were structurally characterized as new structures through extensive 1D and 2D NMR analysis. The water solubility of obtained products <strong>3</strong>–<strong>5</strong> were enhanced by 36–340 times compared to the substrate. Moreover, the antioxidant assay revealed that product <strong>3</strong> exhibited improved 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity compared to the substrate, decreasing the logIC<sub>50</sub> from 10.77 ± 0.05 μM to 9.55 ± 0.05 μM. Compound <strong>3</strong> also displayed significantly higher anticancer activity than the substrate 2′-hydroxyflavanone against Glioblastoma 33 cancer stem cells (GSC33), decreasing the IC<sub>50</sub> from 25.05 μM to 7.07 μM. Thus, <em>S. coeruleorubidus</em> NRRL B-2569 stands out as an effective tool for modifying flavonoids, thereby enhancing their water solubility and bioactivities.</div></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"139 3","pages":"Pages 172-181"},"PeriodicalIF":2.3,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142872239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of bacteriophage propagation in high-yield continuous culture (cellstat) meeting the constraints of industrial manufacturing processes 优化噬菌体在高产连续培养（cellstat）中的繁殖，以满足工业生产过程的限制。

IF 2.3 4区生物学

Journal of bioscience and bioengineering Pub Date : 2024-10-05 DOI: 10.1016/j.jbiosc.2024.09.001

Céleste Caffin , Lhéa Milhamont , Eva Duriez , Agathe Hembert , Pauline Huzet , Camille Lerouge , Marie Deblieck , Denis Watier

{"title":"Optimization of bacteriophage propagation in high-yield continuous culture (cellstat) meeting the constraints of industrial manufacturing processes","authors":"Céleste Caffin , Lhéa Milhamont , Eva Duriez , Agathe Hembert , Pauline Huzet , Camille Lerouge , Marie Deblieck , Denis Watier","doi":"10.1016/j.jbiosc.2024.09.001","DOIUrl":"10.1016/j.jbiosc.2024.09.001","url":null,"abstract":"<div><div>The growing use of bacteriophages in the fields of agriculture, agri-food, veterinary treatments, and medicine involves the quantitative production of these bacteriophages. In this study, we propose a bacteriophage production protocol that can easily be transposed to industry. We used a cellstat production system because the latest studies have shown that it is the most suitable process for the production of phages due to volumetric productivity, safety (limitation of co-evolution), and flexibility (choice of growth rate criteria). Sizing of the assembly used makes it possible to extrapolate the results to industrial production. The production conditions are indicated precisely, which would allow manufacturers to adapt the protocol to their own equipment. We propose experimental conditions in order to obtain a stable <em>Escherichia coli</em> population, qualitatively and over time, and production of high-titer T7 bacteriophages. The optimized production conditions (yield, cost and simplicity of the process) are: a buffered peptone water medium concentration of 11 g L<sup>−1</sup> and a dilution rate of 1.6 h<sup>−1</sup>. Under these conditions, we obtained a production of 7.35×10<sup>16</sup> plaque-forming units (PFU) L<sup>−1</sup> day<sup>−1</sup> with a concentration of 9.8×10<sup>12</sup> PFU mL<sup>−1</sup>. The strength of this work lies in its focus on industrial applicability.</div></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"138 6","pages":"Pages 507-514"},"PeriodicalIF":2.3,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142377847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of sodium metabisulfite treatment and storage condition on metabolic profile of young coconut (Cocos nucifera L.) 焦亚硫酸钠处理和储存条件对椰子（Cocos nucifera L.）新陈代谢的影响

IF 2.3 4区生物学

Journal of bioscience and bioengineering Pub Date : 2024-09-28 DOI: 10.1016/j.jbiosc.2024.08.002

Della Rahmawati , Mary Faith Yamballa Adan , Muhammad Maulana Malikul Ikram , Marvin Nathanael Iman , Eiichiro Fukusaki , Sastia Prama Putri

{"title":"Effect of sodium metabisulfite treatment and storage condition on metabolic profile of young coconut (Cocos nucifera L.)","authors":"Della Rahmawati , Mary Faith Yamballa Adan , Muhammad Maulana Malikul Ikram , Marvin Nathanael Iman , Eiichiro Fukusaki , Sastia Prama Putri","doi":"10.1016/j.jbiosc.2024.08.002","DOIUrl":"10.1016/j.jbiosc.2024.08.002","url":null,"abstract":"<div><div>Young coconuts (<em>Cocos nucifera</em> L.) used for export are trimmed to reduce their size and weight to lower transport costs. However, trimmed coconuts have a shorter shelf life due to microbial spoilage and surface discoloration caused by enzymatic browning. To minimize these effects, trimmed coconuts were dipped in an anti-browning agent, sodium metabisulfite (SMB), and stored under ambient conditions. However, there have been no reports on the effects of SMB treatment on metabolome changes in the flesh and water of young coconuts. Hence, this study investigated the metabolite changes in trimmed young coconuts after SMB treatment under different storage conditions using a gas chromatography (GC)/mass spectrometry (MS) metabolomic profiling approach. Tall young coconut samples were trimmed and treated with a 2% SMB solution for 5 min before storage at 25 °C or 4 °C for 2–4 weeks. Coconut flesh and water samples were collected after storage for 0, 2, and 4 weeks, and were subjected to GC–MS analysis. The results showed that the major metabolites affected by coconut deterioration were amino acids, sugars, and sugar alcohols. SMB treatment and/or refrigeration can help prevent metabolite changes in the flesh and water of young coconuts. In the future, improvements in storage conditions based on metabolite profiles should be explored.</div></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"138 6","pages":"Pages 515-521"},"PeriodicalIF":2.3,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142347274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0