Journal of bioscience and bioengineering最新文献

{"title":"Glycolytic inhibition by resveratrol facilitates chondrocyte survival under glucose-deprived conditions and improves the viability of 3D-cultured cartilage tissue","authors":"Rui Zhang,&nbsp;Keita Kanki","doi":"10.1016/j.jbiosc.2025.05.001","DOIUrl":"10.1016/j.jbiosc.2025.05.001","url":null,"abstract":"<div><div>Decreased cell viability resulting from severe nutrient deprivation is a major obstacle in three-dimensional (3D) tissue construction. Therefore, technical improvements that prevent cell death in the core region of cell aggregates are desired for the development of large, thick tissues. We focused on the anti-glycolytic effects of resveratrol (RSV), a polyphenol known as a caloric restriction mimetic, and investigated its cytoprotective effects under glucose-deprived conditions in two-dimensional (2D) and 3D-cell culture systems using rat chondrocytes. In 2D culture, the low-glucose (LG, 0.5 mg/mL) condition caused time- and dose-dependent cell death in chondrocytes, whereas co-treatment with 50 μM RSV significantly restored cell viability under glucose deprivation. In RSV-treated cells, the expression levels of glycolytic genes (<em>GLUT1</em>, <em>PKM</em>, and <em>LDHA</em>) and glucose uptake were significantly downregulated, and phospho-AMPK levels were upregulated, indicating energy stress. RSV treatment restored the expression of extracellular matrix genes (<em>COL1A1</em> and <em>COL2A1</em>), which were downregulated under the LG condition, and augmented the pro-chondrogenic effect of TGF-β1 and ascorbic acid. In a 3D-culture model, spheroids constructed with RSV-pretreated chondrocytes had a more viable core region than dimethyl-sulfoxide-treated control spheroids. TGF-β-induced cartilage maturation led these spheroids to develop larger and more viable tissues than control spheroids. These results suggested that glycolytic inhibition by RSV decreased chondrocyte glucose usage, thereby preventing cell death caused by glucose deprivation. Our findings provide useful information for improving cell viability under hyponutrition conditions and can be applied to 3D tissue construction.</div></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"140 2","pages":"Pages 90-97"},"PeriodicalIF":2.3,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144142665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extracellular vesicles derived from specific lactic acid bacteria have agonistic activity against formyl peptide receptor 2","authors":"Hirosuke Sugahara,&nbsp;Keitaro Nagayama,&nbsp;Koichi Sashihara,&nbsp;Yasushi Nagatomi","doi":"10.1016/j.jbiosc.2025.04.009","DOIUrl":"10.1016/j.jbiosc.2025.04.009","url":null,"abstract":"<div><div>Lactic acid bacteria (LAB) play important roles in food microbiology and human health. Extracellular vesicles (EVs), which are drug transporters that contain functional components, derived from LAB have been recognized as materials with various beneficial effects, such as their anti-inflammatory effects. Owing to the complexity arising from strain-dependent functional differences in LAB, the functions of EVs derived from LAB have not been well elucidated. To investigate the strain-specific functions of EVs from LAB, we evaluated the ability of EVs from different LAB to induce interleukin 10 (IL-10) secretion by M2-like macrophages. The use of EVs with an increased capacity to induce IL-10 secretion and a G protein-coupled receptor (GPCR) assay revealed that EVs derived from specific lactic acid bacterial strains have agonistic effects against formyl peptide receptor 2 (FPR2). A strong and significant correlation between the ability of EVs derived from LAB to induce IL-10 secretion and agonistic activity against FPR2 was identified, and treatment with an FPR2 antagonist reduced the secretion of IL-10 induced by EVs from a specific lactic acid bacterial strain. Ultraviolet B (UVB) irradiation-induced damage to human keratinocytes was reversed after treatment with EVs with agonistic activity against FPR2, and this restorative effect was abolished by treatment with an FPR2 antagonist. In this study, we demonstrate for the first time that EVs derived from specific LAB have agonistic activity against FPR2; this activity could be a crucial factor in the anti-inflammatory effects of EVs released from specific LAB.</div></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"140 1","pages":"Pages 21-27"},"PeriodicalIF":2.3,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144086247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acetate-mediated two-stage microbial production of poly[(R)-3-hydroxybutyrate] (PHB) from CO2 and H2 using a synthetic medium free of vitamins and cysteine","authors":"Huan Ren ,&nbsp;Kazuaki Ninomiya","doi":"10.1016/j.jbiosc.2025.04.004","DOIUrl":"10.1016/j.jbiosc.2025.04.004","url":null,"abstract":"<div><div>The objective of this study is to use the thermophilic acetogen <em>Thermoanaerobacter kivui</em> and a vitamin- and cysteine-free synthetic medium for an acetate-mediated two-stage microbial production of the biodegradable polymer poly[(<em>R</em>)-3-hydroxybutyrate] (PHB) from CO₂ and H₂. In the first stage, <em>T. kivui</em> could produce 10 g/L acetate within 14 days during anaerobic chemoautotrophic culture with substrate gas (H₂:CO₂ = 80:20) using cysteine-free DSMZ 171 medium (originally vitamin-free) in the presence of the pH neutralizer CaCO₃. In the second stage, the acetate-containing spent medium obtained from the vitamin- and cysteine-free chemoautotrophic culture of <em>T. kivui</em> could be used for the aerobic culture of <em>Cupriavidus necator</em> for PHB production. Specifically, <em>C. necator</em> consumed all of the 5 g/L acetate in the spent medium of <em>T. kivui</em> for 36 h. The cell concentration of <em>C. necator</em> reached 1.2 g/L after 30 h of culture. The PHB content of <em>C. necator</em> reached 38 % (=0.46 g-PHB/L). The PHB yield was 0.11 g-PHB/g-acetate used. The present study can contribute to a sustainable production of bioplastic PHB from CO₂ and H₂ without vitamins and amino acids and without the risk of flammability problems caused by combustible gases (mixture of H₂, O₂, CO₂).</div></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"140 1","pages":"Pages 42-49"},"PeriodicalIF":2.3,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144078169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Date, a major dried fruit, extends the lifespan of Caenorhabditis elegans","authors":"Koji Kakugawa ,&nbsp;Shion Uehara ,&nbsp;Syunya Katoh ,&nbsp;Naoki Arimatsu ,&nbsp;Sho Ogaki ,&nbsp;Koji Masumura ,&nbsp;Hideki Murakawa ,&nbsp;Kenji Arakawa ,&nbsp;Masaki Mizunuma","doi":"10.1016/j.jbiosc.2025.04.006","DOIUrl":"10.1016/j.jbiosc.2025.04.006","url":null,"abstract":"<div><div>Dried fruits have many advantages on condensed nutrients, food preservation, and availability through all seasons. This study investigated the longevity effects of five commercially available dried fruits (date, pineapple, fig, mango, and prune) on <em>Caenorhabditis elegans</em>. Each of the five dried fruits remarkably increased the lifespan compared with the control group. Especially, date fruit had the longest mean and maximum lifespan. In the analysis for the ratio of mean lifespan (MLS) to maximum lifespan in order to clarify their relationship, the ratio for date fruit was approximately 0.9, showing that it results in a longer MLS than other dried fruits. These results imply that date fruit consumption leads to a significantly greater increase in the MLS of <em>C</em>. <em>elegans</em>. The date fruit examined in this study did not contain syringic acid, a previously reported factor known to extend lifespan in date fruit, suggesting that other unidentified component(s) may contribute to its longevity-promoting effect.</div></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"140 1","pages":"Pages 36-41"},"PeriodicalIF":2.3,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143998587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineering anaerobic electron flow through heterologous rhodoquinone synthesis in model microbial and photosynthetic platforms","authors":"Haruki Sasaki ,&nbsp;Yuri Yanagihara ,&nbsp;Yui Satofuka ,&nbsp;Masaki Hasegawa ,&nbsp;Yuichiro Kashiyama ,&nbsp;Kimitoshi Sakamoto ,&nbsp;Yoshihiro Toya ,&nbsp;Hiroshi Shimizu ,&nbsp;Takayuki Fujiwara ,&nbsp;Shin'ya Miyagishima ,&nbsp;Mitsuhiro Ueda ,&nbsp;Tatsuji Sakamoto ,&nbsp;Masami Nakazawa","doi":"10.1016/j.jbiosc.2025.04.008","DOIUrl":"10.1016/j.jbiosc.2025.04.008","url":null,"abstract":"<div><div>Anaerobic conditions facilitate bioproduction by enabling diverse metabolic pathways; however, they disrupt redox balance due to the accumulation of reduced cofactors, limiting metabolic efficiency. Rhodoquinone (RQ), a low-redox-potential quinone, supports electron transport under anaerobic conditions. Unlike menaquinone, RQ is synthesized from ubiquinone through a single enzymatic reaction catalyzed by rhodoquinone biosynthesis protein A (RquA), making it a simple, adaptable metabolic engineering tool. In this study, RQ was synthesized in the menaquinone-deficient <em>Escherichia coli</em> Δ<em>menA</em> strain via heterologous gene expression of <em>rquA</em> from <em>Euglena gracilis</em>. The engineered strain tripled succinate production under anaerobic conditions compared with the control strain. Redox analysis showed a decreased NADH/NAD⁺ ratio, reflecting improved electron flow under oxygen-limited conditions. Introducing <em>rquA</em> into a strain with high succinate production further increased succinate yields, confirming compatibility with existing metabolic modifications. To explore broader applications, <em>rquA</em> from <em>Rhodospirillum rubrum</em> was expressed in <em>Cyanidioschyzon merolae</em> mitochondria using a construct with a <em>C. merolae</em> mitochondrial targeting signal. Quinone analysis confirmed RQ synthesis, and the engineered strains produced more succinate anaerobically relative to the controls. Although redox cofactor ratios in <em>C. merolae</em> remained stable, rotenone sensitivity indicated altered mitochondrial electron transport under anaerobic conditions. These findings demonstrate that RQ synthesis enhances anaerobic metabolism in bacterial and eukaryotic systems, providing a versatile tool for metabolic engineering under oxygen-limited conditions.</div></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"140 1","pages":"Pages 14-20"},"PeriodicalIF":2.3,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143965689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative metabolome analysis of sake yeast with enhanced fermentation performance in sake fermentation conditions","authors":"Kotaro Mori ,&nbsp;Taisuke Seike ,&nbsp;Nobuyuki Okahashi ,&nbsp;Toshinari Takahashi ,&nbsp;Fumio Matsuda","doi":"10.1016/j.jbiosc.2025.04.001","DOIUrl":"10.1016/j.jbiosc.2025.04.001","url":null,"abstract":"<div><div>Japanese sake is fermented with specific strains of budding yeast <em>Saccharomyces cerevisiae</em>. Sake yeasts can allow the ethanol concentration of sake to exceed 20 % without distillation. While the genetic mutations responsible for these exceptional properties have been investigated, the underlying metabolism has not been fully explored. It is because yeast cells cultured in sake mash are difficult to collect for metabolome analysis. This study aimed to clarify the metabolic differences of K701 sake yeast and the X2180 diploid laboratory strain when cultured under sake fermentation conditions. To find an alternative medium that mimics sake fermentation and is applicable to measurements of intracellular metabolome, we compared three liquid media, including SD2 medium (synthetic dextrose medium containing 2 % glucose), SD20 medium (synthetic dextrose medium containing 20 % glucose and 1.8 % lactic acid) and pseudo-sake medium (a supernatant of saccharified rice supplemented with 1.8 % lactic acid). Culture profile data demonstrated that the pseudo-sake medium successfully reproduced the metabolic traits of K701 observed in sake mash. Targeted metabolome analysis of yeast cells cultured in the pseudo-sake medium revealed that levels of glycolytic metabolites, such as glucose-6-phosphate (G6P), fructose-6-phosphate (F6P), and fructose-1,6-bisphosphate (FBP), were significantly higher with K701. Based on metabolite concentration data, we inferred that K701 cells had a higher ATP regeneration rate. Calculation of differential Gibbs free energy changes revealed that the glucokinase reaction was upregulated in K701. The present study has, for the first time, revealed the metabolism of K701 sake yeast responsible for its exceptional fermentation ability under sake fermentation conditions.</div></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"140 1","pages":"Pages 28-35"},"PeriodicalIF":2.3,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144019694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lysine source for ε-poly-l-lysine biosynthesis depends on diaminopimelate pathway during its production in Streptomyces albulus","authors":"Fumihito Hasebe ,&nbsp;Daisuke Shimada ,&nbsp;Chitose Maruyama ,&nbsp;Yoshimitsu Hamano","doi":"10.1016/j.jbiosc.2025.04.005","DOIUrl":"10.1016/j.jbiosc.2025.04.005","url":null,"abstract":"<div><div><em>Streptomyces albulus</em> NBRC14147 produces the polycationic homopoly(amino acid) ε-poly-<span>l</span>-lysine (ε-PL). Due to its antimicrobial properties, nontoxicity to humans, biodegradability, and permeability, there is a high demand for ε-PL. As ε-PL is produced by <span>l</span>-lysine polymerization, elucidating the source of <span>l</span>-lysine for ε-PL production is crucial for enhancing its yield. In actinobacteria, <span>l</span>-lysine is produced by diaminopimelate (DAP) pathway. In this study, 2,6-pyridine-dicarboxylate (PDC) was identified as the inhibitor of DapB, a DAP pathway enzyme, by comparing the structure of DapB from <em>Mycobacterium tuberculosis</em> with the model structure of DapB from <em>S. albulus</em>. We also found that adding PDC inhibited the growth of <em>S. albulus</em>. More importantly, PDC additions during the initial stages of the ε-PL production phase led to the accumulation of amino acids generated from pyruvate and <span>l</span>-aspartic 4-semialdehyde, while the ε-PL production was terminated. These findings suggest that de novo biosynthesized nascent <span>l</span>-lysine from the DAP pathway contributes to ε-PL production.</div></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"140 1","pages":"Pages 9-13"},"PeriodicalIF":2.3,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143993134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing azo dye degradation using dual enzyme systems immobilized on a mesoporous silica scaffold","authors":"Shun-ichi Matsuura ,&nbsp;Takeshi Ikeda ,&nbsp;Manami Chiba ,&nbsp;Aritomo Yamaguchi","doi":"10.1016/j.jbiosc.2025.04.003","DOIUrl":"10.1016/j.jbiosc.2025.04.003","url":null,"abstract":"<div><div>Highly efficient degradation of the azo dye methyl red has been achieved through a coupled enzymatic reaction using mesoporous silica as an immobilization scaffold for two different enzymes. The dispersion of the non-immobilized free azoreductase (AzoR) and glucose dehydrogenase (GDH) in the reaction solution caused vigorous aggregation of these heterologous enzymes, resulting in their deactivation. In the present study, we aimed to overcome this limitation by fusing Si-tag, a silica-binding protein, to both enzymes and simultaneously immobilizing them on the surface of highly ordered pores of mesoporous silica. Consequently, the degradation ratio of methyl red significantly increased with immobilization on the mesoporous silica compared to that from immobilization on a non-porous silica support. However, the immobilization of the AzoR and GDH with and without the Si-tag, respectively, on silica markedly decreased the enzyme activity during the reusability test owing to the desorption of GDH involved in coenzyme regeneration. By contrast, the activities of the two enzymes immobilized on the mesoporous silica surface markedly increased upon fusion of both with Si-tag. Furthermore, these dual enzyme–mesoporous silica composites prepared at high salt and surfactant concentrations exhibited higher durability and repeatability during methyl red degradation than when using non-porous silica.</div></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"140 1","pages":"Pages 1-8"},"PeriodicalIF":2.3,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143997963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation of hair follicle germs using centrifugal forces for hair regenerative medicine","authors":"Yuki Migita ,&nbsp;Tatsuto Kageyama ,&nbsp;Naoya Ito ,&nbsp;Naoki Esaka ,&nbsp;Ayaka Nanmo ,&nbsp;Jieun Seo ,&nbsp;Yan Lei ,&nbsp;Sayuri Hamano ,&nbsp;Junji Fukuda","doi":"10.1016/j.jbiosc.2025.04.002","DOIUrl":"10.1016/j.jbiosc.2025.04.002","url":null,"abstract":"<div><div>Hair regenerative medicine presents a potential approach to treat hair loss. Mesenchymal and epithelial cells may be transplanted to regenerate <em>de novo</em> hair follicles, as epithelial–mesenchymal interactions are crucial for hair follicle morphogenesis. However, transplanting a mixture of the two cell types does not lead to efficient hair follicle formation, and engineering tissue grafts with these cell types before transplantation is necessary. Hair follicle germ-like aggregates (HFGs), which are found during hair follicle development, induced highly efficient <em>de novo</em> hair follicle formation. Although this is a sophisticated approach of mimicking <em>in vivo</em> hair follicle morphogenesis, further studies are required owing to its laborious and time-consuming nature. This study proposed a straightforward approach to prepare HFGs using centrifugal forces. We fabricated a centrifugal device consisting of tube tips that facilitate cell transplantation as a high cell dense aggregate, in contrast to conventional cell suspension injections. To prepare HFGs, mouse embryonic epithelial and mesenchymal cells were packed into the device using a two-step centrifugation method. Immediately after preparation, HFGs were directly injected into the back skin of nude mice, resulting in <em>de novo</em> hair follicle formation. This centrifugal approach significantly improved hair follicle regeneration efficiency compared with that of conventional cell suspension injection. Unlike previous studies, this approach does not require several days of culture, which could potentially facilitate rapid and cost-effective hair regenerative medicine.</div></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"139 6","pages":"Pages 445-450"},"PeriodicalIF":2.3,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144016479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Data-independent acquisition-based lipidomics reveals lipidome alterations associated with growth of Saccharomyces cerevisiae at low temperature","authors":"Daiki Hara ,&nbsp;Nobuyuki Okahashi ,&nbsp;Taisuke Seike ,&nbsp;Junko Iida ,&nbsp;Fumio Matsuda","doi":"10.1016/j.jbiosc.2025.03.004","DOIUrl":"10.1016/j.jbiosc.2025.03.004","url":null,"abstract":"<div><div>Budding yeast, <em>Saccharomyces cerevisiae</em>, adapts to low-temperature stress by altering the amounts and proportions of phospholipids, as well as the structures of acyl chains. To better understand this process, a data-independent acquisition-based lipidomics using liquid chromatography–quadrupole time-of-flight mass spectrometry method was developed to quantify acyl chain isomers in glycerolipids and phospholipids. This method distinguished lipid molecular species, such as phosphatidylcholine (PC) 16:1_18:0 and PC16:0_18:1, at the level of acyl chain isomers. These species were previously identified only at the total acyl chain level as PC 34:1 using the conventional data-dependent acquisition (DDA) method. Using this approach, 145 diacyl lipid molecular species were identified in <em>S. cerevisiae</em> at 30 °C, compared to only 101 identified using the conventional DDA method. Analysis of <em>S. cerevisiae</em> at 10 °C revealed 121 phospholipid and diacylglycerol (DG) molecular species and 260 triacylglycerol (TG) features as well as other 51 sphingolipids and sterol lipids. The results demonstrated unique increases in phosphatidylinositol (PI), phosphatidylethanolamine (PE), monomethyl (MM) PE, and TG containing both 16:1 and medium-chain fatty acids (MCFAs). The knockout strains <em>ole1Δ</em> and <em>slc1Δ</em>, lacking the desaturase that produces 16:1 and the acyltransferase favoring MCFAs, respectively, exhibited growth defects exclusively while growing at 10 °C. These findings suggest that <em>S. cerevisiae</em> regulates its lipid profiles to address low-temperature stress.</div></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"139 6","pages":"Pages 414-423"},"PeriodicalIF":2.3,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143993132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}