Journal of bioscience and bioengineering最新文献

{"title":"Topical delivery of gel-in-oil emulsion cocktail with growth factors for the treatment of diabetic pressure ulcers","authors":"Yi Zhang ,&nbsp;Jannatul Fardous ,&nbsp;Yu Zhou ,&nbsp;Lichun Wu ,&nbsp;Ryota Doi ,&nbsp;Jia Hu ,&nbsp;Yuya Hirota ,&nbsp;Yasuhiro Ikegami ,&nbsp;Shinichi Aishima ,&nbsp;Hiroyuki Ijima","doi":"10.1016/j.jbiosc.2024.10.011","DOIUrl":"10.1016/j.jbiosc.2024.10.011","url":null,"abstract":"<div><div>Healing diabetic foot ulcers (DFUs) poses a serious challenge for many individuals with diabetes. The use of biomaterials applied locally for treating DFUs has recently garnered significant attention. Here, we present a gel-in-oil nanogel dispersion (G/O-NGD) capable of local delivery of six different growth factors (GFs) via the topical route, followed by an in-vivo evaluation in mice. Both macroscopic and microscopic changes in skin structure were evidented after topical application of GF-cocktail G/O-NGD, and changes in CD68 and CD31 levels and collagen content were measured. Expression and synthesis of Interleukin-6 (IL-6), transforming GF beta 1 (TGF-β1), and basic fibroblast GF (bFGF) were also analyzed. The results showed that a significant reduction in ulcer area, restoration of skin structure, increase in collagen content, angiogenesis, and suppression of inflammation were possible with GF-cocktail G/O-NGD, indicating that G/O-NGD is a prospective carrier for local delivery of GF, improving wound healing processes.</div></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"139 2","pages":"Pages 112-122"},"PeriodicalIF":2.3,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142739786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective role of hematin in alcohol oxidase-mediated glycerol oxidation: A kinetic modeling approach","authors":"Adrián Parodi ,&nbsp;Agostina Córdoba ,&nbsp;Mariano Asteasuain ,&nbsp;Ivana Magario","doi":"10.1016/j.jbiosc.2024.12.012","DOIUrl":"10.1016/j.jbiosc.2024.12.012","url":null,"abstract":"<div><div>Mathematical modeling of complex multi-catalytic reaction pathways is a powerful tool not only for determining optimal operational conditions but also for gaining insights into the kinetic behavior of efficient yet product-sensitive enzymes. This approach was applied to the biooxidation of glycerol using oxidases, aided by hematin, a natural mimic of heme peroxidases and catalases, which acts as a scavenger for enzymatically produced H₂O₂. The bi-catalytic reaction system was coupled with the formation of a colored imine product from phenol and 4-aminoantipyrine, enabling spectrophotometric monitoring of reaction kinetics. Alcohol oxidase (AOX) from <em>Komagataella pastoris</em> (formerly <em>Pichia pastoris</em>) was submitted to this strategy to overcome some kinetic drawbacks previously observed with galactose oxidase (GAO) from <em>Dactylium dendroides</em>. Although both enzymes exhibited comparable oxidation yields, computer-aided kinetic analyses revealed significant differences in efficiency and sensitivity to H₂O₂. AOX demonstrated two orders of magnitude greater efficiency and a similarly higher affinity for glycerol. However, AOX showed a stronger tendency to bind H₂O₂ compared to hematin or GAO, as suggested by the ratio of the inhibition constant (<em>k</em>_inh) to the activation constant (<em>k</em>₁) for hematin. The kinetic model was also used to simulate optimal conditions for enhancing the stability of AOX and hematin. Reducing glycerol concentration or increasing hematin concentration improved AOX stability, while the addition of phenol helped preserve hematin. The predicted stabilities turn the AOX/hematin/phenol system promising for biosensing or analytical applications.</div></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"139 4","pages":"Pages 263-270"},"PeriodicalIF":2.3,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143074513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gas chromatography–mass spectrometry metabolic profiling and sensory evaluation of greenhouse mangoes (Mangifera indica L. ‘Irwin’) over multiple harvest seasons","authors":"Miwa Sato ,&nbsp;Eiichiro Fukusaki","doi":"10.1016/j.jbiosc.2024.12.013","DOIUrl":"10.1016/j.jbiosc.2024.12.013","url":null,"abstract":"<div><div>Compared to outdoor mango cultivation in the tropics, greenhouse cultivation in temperate regions is less reported due to its short history and small scale. Here, we evaluated for the first time the taste-focused quality of greenhouse-grown mangoes (Irwin) by GC–MS metabolic profiling and sensory evaluation for over three years (2021–2023). The relative standard deviation in sensory evaluation scores was approximately 15 % each year. Meanwhile, principal component analysis was performed on 45 identified metabolites and clustered with similar topology over three years. Orthogonal partial least squares regression analysis showed that a prediction model could be constructed with R₂, Q₂ = 0.9 or higher for all three years. Furthermore, the 2021 prediction model was used to evaluate the accuracy of sensory evaluation scores in a different year (2023). Compared to the model that used all 45 metabolites as explanatory variables, the accuracy of the model improved when using only 24 important metabolites which are common to both years (2021 and 2022), suggesting that these metabolites are highly reproducible across years. This study would contribute not only to fundamental greenhouse information, but also to the improvement of quality and cultivation methods in greenhouse in the future.</div></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"139 4","pages":"Pages 280-287"},"PeriodicalIF":2.3,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143059100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ROS-responsive nanocarrier for oral delivery of monascin and enhanced alleviation of oxidative stress","authors":"Xueyi Chen ,&nbsp;Wenli Dai ,&nbsp;Wanqi Liang ,&nbsp;Dong Li ,&nbsp;Wo-Qi Cai ,&nbsp;Chan Zhang ,&nbsp;Wenlin Hu ,&nbsp;Bo-Bo Zhang ,&nbsp;Qiongqiong Yang","doi":"10.1016/j.jbiosc.2025.01.003","DOIUrl":"10.1016/j.jbiosc.2025.01.003","url":null,"abstract":"<div><div>Oxidative stress, caused by excessive production of reactive oxygen species (ROS), plays a crucial role in the occurrence and development of various diseases. Monascin can scavenge ROS and alleviate oxidative stress but with a low fermentation rate and bioavailability. Here, we optimized the fermentation process to increase the production of monascin (508.6 U/mL), and then systematically characterized its structure via HPLC, HPLC-MS, ¹H NMR, and ¹³C NMR. Additionally, we innovatively modified carboxymethylcellulose sodium with selenium (CMC-Se) to encapsulate monascin (monascin@CMC-Se), which can sensitively respond to ROS and release monascin to effectively scavenge excessive ROS. Besides, the monascin@CMC-Se can significantly increase the bioaccessibility of monascin and alleviate cellular oxidative stress by enhancing its cellular uptake rate. Collectively, our work provides proof-of-principle evidence that the CMC-Se can precise delivery of monascin to an oxidatively stressed environment with high resistance to gastric fluids, laying a foundation to overcome inflammation-related diseases in the colon.</div></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"139 4","pages":"Pages 319-328"},"PeriodicalIF":2.3,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143046881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial protein production during fermentation of starch-rich legume flours using Aspergillus oryzae and Lactobacillus plantarum starter cultures","authors":"Yuriy Kryachko ,&nbsp;Lashmitha Arasaratnam ,&nbsp;James Duncan House ,&nbsp;Yongfeng Ai ,&nbsp;Michael Todd Nickerson ,&nbsp;Darren Raymond Korber ,&nbsp;Takuji Tanaka","doi":"10.1016/j.jbiosc.2024.12.015","DOIUrl":"10.1016/j.jbiosc.2024.12.015","url":null,"abstract":"<div><div>Starch-rich faba bean, yellow lentil, and yellow field pea flours were subjected to submerged fermentation using <em>Aspergillus oryzae</em> and <em>Lactobacillus plantarum</em> starter mono- or co-cultures, to increase protein contents of the flours. Fermentation mixes were supplemented with up to 35 g/L urea, ammonium sulfate and/or monoammonium phosphate as nitrogen sources. Protein contents of the flours increased 2–2.5-fold, i.e., total protein contents of up to 33 % were achieved following fermentation with <em>A. oryzae</em> monoculture or <em>A. oryzae</em>‒<em>L. plantarum</em> co-culture. Therefore, <em>A. oryzae</em> was capable of efficient legume starch utilization and protein production. Using <em>A. oryzae</em>‒<em>L. plantarum</em> co-culture led to the highest yields of at least six of microbially produced amino acids. After fermentation by <em>L. plantarum</em> monoculture, protein contents remained largely unchanged. However, <em>in vitro</em> protein digestibility of flours improved to a greater extent after fermentation with <em>L. plantarum</em> monoculture than with either <em>A. oryzae</em> monoculture or the co-culture. Hence, during fermentation with <em>L. plantarum</em> monoculture, microbially mediated hydrolysis of legume protein (generating smaller and easier digestible peptides), rather than microbial starch utilization, was the predominant process.</div></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"139 4","pages":"Pages 288-295"},"PeriodicalIF":2.3,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143038863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Monitoring and optimization of the microenvironment in a gravity-driven microfluidic system placed on a slow-tilting table","authors":"Nuttakrit Limjanthong ,&nbsp;Shinji Sugiura ,&nbsp;Taira Oda ,&nbsp;Fuko Takusari ,&nbsp;Yasunori Fujiwara ,&nbsp;Toshimasa Miyazaki ,&nbsp;Kosei Naganuma ,&nbsp;Kiyoshi Ohnuma","doi":"10.1016/j.jbiosc.2024.12.014","DOIUrl":"10.1016/j.jbiosc.2024.12.014","url":null,"abstract":"<div><div>Gravity-driven microfluidic chips offer portability and flexibility in different settings because pumps and connecting tubes are unnecessary for driving fluid flow. In a previous study, human induced pluripotent stem cells were cultured using gravity-driven microfluidics, with the liquid flow rate regulated by a tilting table. However, instability in cell culture has been observed, occasionally leading to cell death owing to unknown causes. This study measured the ability of a gravity-driven microfluidic system to maintain essential microenvironments, specifically the flow rate, CO₂ levels, temperature, and humidity. The incubation procedure was improved to stabilize the parameters at target values. Improvements in the incubation process reduced the time required to reach the stabilized value for CO₂, temperature, and humidity by 85, 67, and 5 %, respectively, compared to previous methods. The system demonstrated a precise flow rate, confirmed by a consistent increase in the downstream tank's medium volume after 4 h of perfusion. In addition, the adjustment of the tilting table maintained a steady angle and effectively regulated the flow rate, with the measured flow rate consistent with the theoretical value. The gravity-driven microfluidic system effectively facilitated the culture and differentiation of human iPSCs into the mesodermal lineage after bone morphogenetic protein 4 induction, as indicated by positive SSEA1 immunostaining, demonstrating its potential for stem cell research. Gravity-driven microfluidic systems satisfy these requirements and are suitable for stem cell culture experiments.</div></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"139 4","pages":"Pages 311-318"},"PeriodicalIF":2.3,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143023592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Crucial role of early addition of Wickerhamiella versatilis in enhancing aroma formation during soy sauce fermentation","authors":"Yuichi Mizuno ,&nbsp;Takashi Yoshimura ,&nbsp;Kazutaka Sawada ,&nbsp;Keisuke Tsuge ,&nbsp;Yukio Nagano ,&nbsp;Yumiko Yoshizaki ,&nbsp;Masatoshi Goto ,&nbsp;Genta Kobayashi","doi":"10.1016/j.jbiosc.2024.12.010","DOIUrl":"10.1016/j.jbiosc.2024.12.010","url":null,"abstract":"<div><div>In modern Japanese soy sauce production, sealed outdoor fermentation tanks are used to ferment <em>moromi</em> with halotolerant starter cultures: the lactic acid bacterium <em>Tetragenococcus halophilus</em> and yeasts <em>Wickerhamiella versatilis</em> and <em>Zygosaccharomyces rouxii</em>. <em>T. halophilus</em> and <em>W. versatilis</em> are added in the early fermentation stage, while <em>Z. rouxii</em> is introduced about 1 month later to initiate alcoholic fermentation. Both <em>W. versatilis</em> and <em>Z. rouxii</em> contribute to the production of volatile organic compounds (VOC), with 4-ethylguaiacol (4-EG), uniquely produced by <em>W. versatilis</em>, being essential for the characteristic aroma of soy sauce. Prior metagenomic and metabolomic analyses indicated that 4-EG production occurs prior to <em>Z. rouxii</em> fermentation, though the increase in <em>W. versatilis</em> proportion follows it. Additional omics analysis of a production batch confirmed similar microbial and VOC dynamics, with no clear relationship between <em>W. versatilis</em> increase and 4-EG levels. To investigate this, a laboratory-scale experiment was conducted using filter-sterilized <em>moromi</em> supernatant as a medium, with staggered inoculations of the two yeasts. Viable cell density, 4-EG, and ethanol were measured as indicators of fermentation activity. Results showed that when <em>W. versatilis</em> was inoculated before <em>Z. rouxii</em>, 4-EG production commenced earlier, while <em>W. versatilis</em> cell density and ethanol production increased only after <em>Z. rouxii</em> fermentation began. Under these conditions, 4-EG and ethanol production were highest, suggesting that the presence rather than the increase of <em>W. versatilis</em> is crucial for 4-EG production. Consequently, the early addition of <em>W. versatilis</em> was considered an effective strategy to enhance 4-EG and VOC production in <em>moromi</em>.</div></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"139 4","pages":"Pages 271-279"},"PeriodicalIF":2.3,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143005854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physiological role of the FDH1 gene in methylotrophic yeast Komagataella phaffii and carbon recovery as formate during methylotrophic growth of the fdh1-deletion strain","authors":"Junzhang Zhu ,&nbsp;Hao-Liang Cai ,&nbsp;Shunta Goto ,&nbsp;Masaya Shimada ,&nbsp;Hiroya Yurimoto ,&nbsp;Yasuyoshi Sakai ,&nbsp;Takehiko Yoko-o ,&nbsp;Yasunori Chiba ,&nbsp;Tomoyuki Nakagawa","doi":"10.1016/j.jbiosc.2024.12.007","DOIUrl":"10.1016/j.jbiosc.2024.12.007","url":null,"abstract":"<div><div>During methylotrophic growth of <em>Komagataella phaffii</em>, a large amount of carbon is lost as CO₂. In this study, we aimed to construct a recovery system for carbon atoms, which emit as CO₂ along the methanol dissimilation pathway in the form of formate when using strain <em>fdh1</em>Δ, the deletion mutant of formate dehydrogenase gene (<em>FDH1</em>). Strain <em>fdh1</em>Δ showed a severe growth defect when using methanol as the sole carbon source. However, using YPM medium (1 % yeast extract, 2 % peptone and 1 % methanol) with pH adjustment, the strain recovered methylotrophic growth and methanol utilization, accumulating high levels of extracellular formate in the medium. In strain <em>fdh1</em>Δ, the final carbon conversion rate of formate from methanol reached 12.9 % by batch cultivation. Moreover, by fed-batch cultivation in a jar fermentor, the formate productivity of strain <em>fdh1</em>Δ achieved approximately 540 mM. These results indicated that strain <em>fdh1</em>Δ possessed a high potential for recovering carbon atoms as formate, which can be a valuable precursor for various industrial applications.</div></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"139 3","pages":"Pages 206-212"},"PeriodicalIF":2.3,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143005856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of transient change in temperature by daki warm treatment on the growth of bacteria during kimoto-style seed mash preparation","authors":"Masayuki Takahashi,&nbsp;Kana Morikawa,&nbsp;Takeshi Akao","doi":"10.1016/j.jbiosc.2024.12.011","DOIUrl":"10.1016/j.jbiosc.2024.12.011","url":null,"abstract":"<div><div><em>Daki</em> warm treatment (<em>daki-ire</em>) is performed during the process of seed mash preparation in the brewing of Japanese sake in order to promote the saccharification of rice by <em>koji</em> enzyme and to enhance the growth of <em>Saccharomyces cerevisiae</em>. Although it is important to control the growth of lactic acid bacteria in the preparation of <em>kimoto</em>-style seed mash (traditional sake-brewing method), it has not been known whether the transient increase in the temperature and/or appropriate temperature zone produced by <em>daki-ire</em> influences the growth of bacteria. A temperature increase generally helps bacterial growth, but we have found no published investigation of the influence of temperature changes in <em>daki-ire</em> on bacterial growth during the <em>kimoto</em>-style seed mash preparation process. In this first comprehensive evaluation of the effects of the temperature change by <em>daki-ire</em> on bacterial communities, we investigated the bacterial community in three batches that were brewed by the same brewery using identical ingredients. The results demonstrated that the bacterial community or its transition during lactic acid fermentation was diverse despite the use of the same brewing conditions. We observed that (i) some lactic acid bacteria were carried over to the subsequent batches, and (ii) the increase in the initial amount of lactic acid bacteria plays an important role in the formation of the bacterial community. Our analysis of the bacterial growth activity before and after <em>daki-ire</em> indicated that the transient increase in temperature and/or local appropriate temperature by <em>daki-ire</em>, in and of itself, has relatively little direct impact on bacterial growth.</div></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"139 3","pages":"Pages 219-225"},"PeriodicalIF":2.3,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143005855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fabrication of phospholipid polymer-modified alginate hydrogels for bioartificial pancreas","authors":"Ryo Futatsubashi,&nbsp;Masahiro Kaneko,&nbsp;Akira Ito","doi":"10.1016/j.jbiosc.2024.12.008","DOIUrl":"10.1016/j.jbiosc.2024.12.008","url":null,"abstract":"<div><div>The bioartificial pancreas, composed of a semi-permeable hydrogel encapsulating insulin-secreting cells, has attracted attention as a treatment for type 1 diabetes. In this study, we developed phospholipid polymer-modified alginate hydrogel beads that encapsulated spheroids of the pancreatic beta cell line MIN6. The hydrogel beads were composed of methacrylated alginic acid, which enabled both ionic and covalent cross-linking, resulting in a hydrogel that was more stable than conventional alginate hydrogels. Furthermore, modification of biocompatible 2-methacryloyloxyethyl phosphorylcholine (MPC) polymers suppressed protein adsorption to the hydrogel beads. Hydrogel beads encapsulating MIN6 spheroids maintained the cell viability and insulin secretion ability in response to glucose levels <em>in vitro</em>. Allogenic transplantation of gel beads lowered blood glucose levels in diabetic mice for 30 days, whereas gel beads without MPC polymer modification failed to regulate blood glucose levels. These results indicate that MPC polymer modification of hydrogels provides a new strategy for the fabrication of functional bioartificial pancreas and transplantable biomaterials for cell therapies.</div></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"139 3","pages":"Pages 242-248"},"PeriodicalIF":2.3,"publicationDate":"2025-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142970916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}