Journal of biomedical materials research. Part A最新文献

{"title":"Conductive Microfibers Improve Stem Cell-Derived Cardiac Spheroid Maturation","authors":"Gisselle Gonzalez,&nbsp;Thomas G. Molley,&nbsp;Erin LaMontagne,&nbsp;Alis Balayan,&nbsp;Alyssa R. Holman,&nbsp;Adam J. Engler","doi":"10.1002/jbm.a.37856","DOIUrl":"10.1002/jbm.a.37856","url":null,"abstract":"<div>\u0000 \u0000 <p>Conventional two-dimensional (2D) cardiomyocyte differentiation protocols create cells with limited maturity, which impairs their predictive capacity and has driven interest in three-dimensional (3D) engineered cardiac tissue models of varying maturity and scalability. Cardiac spheroids are attractive high-throughput models that have demonstrated improved functional and transcriptional maturity over conventional 2D differentiations. However, these 3D models still tend to have limited contractile and electrical maturity compared to highly engineered cardiac tissues; hence, we incorporated a library of conductive polymer microfibers in cardiac spheroids to determine if fiber properties could accelerate maturation. Conductive microfibers improved contractility parameters of cardiac spheroids over time versus nonconductive fibers, specifically, when they were short, for example, 5 μm, and when there was moderate fiber mass per spheroid, for example, 20 μg. Spheroids with optimal conductive microfiber length and concentration developed a thicker ring-like perimeter and a less compacted cavity, improving their contractile work compared to control cardiac spheroids. Functional improvements correlated with increased expression of contractility and calcium handling-related cardiac proteins, as well as improved calcium handling abilities and drug response. Taken together, these data suggest that conductive microfibers can improve cardiac spheroid performance to improve cardiac disease modeling.</p>\u0000 </div>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2024-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142886310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Porous Nanocomposite Implants With Gold Nanoparticles for Plastic Surgery","authors":"Olena Korotych,&nbsp;Svitlana Dybkova,&nbsp;Anatolii Maletskyi,&nbsp;Liudmyla Rieznichenko,&nbsp;Liudmyla Kernosenko,&nbsp;Tamara Gruzina,&nbsp;Tetiana Poltoratska,&nbsp;Oleksandr Artiomov,&nbsp;Nataliia Pasmurtseva,&nbsp;Valentyna Podolska,&nbsp;Pavlo Vorotytskiy,&nbsp;Yurii Samchenko","doi":"10.1002/jbm.a.37851","DOIUrl":"10.1002/jbm.a.37851","url":null,"abstract":"<div>\u0000 \u0000 <p>This study presents an innovative approach to improve implant biointegration and reduce implant-associated infections using porous poly(vinyl formal) nanocomposite matrices incorporated with gold nanoparticles and antimicrobial/anticancer drugs for plastic surgery applications. The porous matrices were characterized using physicochemical techniques and in vitro biochemical assays. The results demonstrated the biocompatibility of PVF nanocomposites and their potential for functionalization with various bioactive molecules and drugs, thereby enhancing their therapeutic efficacy. In vivo studies in rabbits revealed progressive replacement of implants with fibrous tissue, indicating successful biointegration with the surrounding soft tissues. Future research will focus on the long-term biocompatibility and functionalization of these nanocomposite implants for plastic surgery and explore their potential in other biomedical applications such as wound healing, tissue engineering, and scaffolds for cell growth and differentiation.</p>\u0000 </div>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142883795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrically Conductive Injectable Silk/PEDOT: PSS Hydrogel for Enhanced Neural Network Formation","authors":"Rajiv Borah,&nbsp;Julia O'Sullivan,&nbsp;Meenakshi Suku,&nbsp;Dahnan Spurling,&nbsp;Daniel Diez Clarke,&nbsp;Valeria Nicolosi,&nbsp;Maeve A. Caldwell,&nbsp;Michael G. Monaghan","doi":"10.1002/jbm.a.37859","DOIUrl":"10.1002/jbm.a.37859","url":null,"abstract":"<p>With no effective treatments for functional recovery after injury, spinal cord injury (SCI) remains one of the unresolved healthcare challenges. Human induced pluripotent stem cell (hiPSC) transplantation is a versatile patient-specific regenerative approach for functional recovery after SCI. Injectable electroconductive hydrogel (ECH) can further enhance the cell transplantation efficacy through a minimally invasive manner as well as recapitulate the native bioelectrical microenvironment of neural tissue. Given these considerations, we report a novel ECH prepared through self-assembly facilitated in situ gelation of natural silk fibroin (SF) derived from mulberry <i>Bombyx mori</i> silk and electrically conductive PEDOT:PSS. PEDOT:PSS was pre-stabilized to prevent the potential delamination of its hydrophilic PSS chain under aqueous environment using 3% (v/v) (3-glycidyloxypropyl)trimethoxysilane (GoPS) and 3% (w/v) poly(ethylene glycol)diglycidyl ether (PeGDE). The resultant ECH formulations are easily injectable with standard hand force with flow point below 100 Pa and good shear-thinning properties. The ECH formulations with unmodified and GoPS-modified PEDOT:PSS, that is, SF/PEDOT and SF/PEDOT<i>GoP</i> maintain comparable elastic modulus to spinal cord (~10–60 kPa) under physiological condition, indicating their flexibility. The GoPS-modified ECHs also display improved structural recoverability (~70%–90%) as compared to the unmodified versions of the ECHs (~30%–80%), as indicated by the three interval time thixotropy (3ITT) test. Additionally, these ECHs possess electrical conductivity in the range of ~0.2–1.2 S/m comparable to spinal cord (1–10 S/m), indicating their ability to mimic native bioelectrical environment. Approximately 80% or more cell survival was observed when hiPSC-derived cortical neurons and astrocytes were encapsulated within these ECHs. These ECHs support the maturation of cortical neurons when embedded for 7 days, fostering the development of a complex, interconnected network of long axonal processes and promoting synaptogenesis. These results underline the potential of silk ECHs in cell transplantation therapy for spinal cord regeneration.</p>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.a.37859","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142886325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sliding Hydrogels Reveal the Modulation of Mechanosensing Attenuates the Inflammatory Phenotype of Osteoarthritic Chondrocytes in 3D","authors":"Manish Ayushman,&nbsp;Hung-Pang Lee,&nbsp;Pranay Agarwal,&nbsp;Georgios Mikos,&nbsp;Xinming Tong,&nbsp;Sarah Jones,&nbsp;Sauradeep Sinha,&nbsp;Stuart Goodman,&nbsp;Nidhi Bhutani,&nbsp;Fan Yang","doi":"10.1002/jbm.a.37861","DOIUrl":"10.1002/jbm.a.37861","url":null,"abstract":"<div>\u0000 \u0000 <p>Osteoarthritis (OA) is a prevalen degenerative joint disease with no FDA-approved therapies that can halt or reverse its progression. Current treatments address symptoms like pain and inflammation, but not underlying disease mechanisms. OA progression is marked by increased inflammation and extracellular matrix (ECM) degradation of the joint cartilage. While the role of biochemical cues has been widely studied for OA, how matrix mechanical cues influence OA phenotype remains poorly understood. Using sliding hydrogels (SGs) as a tool, we examine how local matrix compliance in 3D modulates OA chondrocyte phenotype and associated mechanosensing. We demonstrate that local matrix compliance reduces the inflammatory phenotype of OA chondrocytes, as indicated by decreased gene expression of catabolic markers and proinflammatory cytokine secretion. This is achieved via significantly reduced nuclear NF-κB expression and signaling in OA chondrocytes. Live cell imaging shows enhanced cellular and nuclear dynamics with increased matrix deformation in the compliant SG. Blocking cellular dynamics negates SG compliance-induced benefits in reducing OA inflammatory phenotype. Further, SG alters nuclear mechanosensing in OA as indicated by increased nuclear lamin reinforcement and chromatin condensation. Finally, we demonstrate that a drug inhibiting histone lysine demethylase to modulate chromatin accessibility reduces OA inflammation in 3D hydrogels. These findings advance our understanding of how ECM mechanics regulate OA mechanobiology and progression and highlight potential disease-modifying treatments via epigenetic and mechanosensing-based therapies.</p>\u0000 </div>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142883801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Significant Environmental Factors in the Drift of Electrical Properties in Conductive Nano-Composite Sensors for Biomechanical Motion-Tracking","authors":"Emma E. Bowden,&nbsp;Jacob D. Carter,&nbsp;Anton E. Bowden,&nbsp;Ulrike H. Mitchell,&nbsp;David T. Fullwood","doi":"10.1002/jbm.a.37863","DOIUrl":"10.1002/jbm.a.37863","url":null,"abstract":"<div>\u0000 \u0000 <p>Wearable nanocomposite stretch sensors are an exciting new development in biomaterials for biomechanical motion-tracking technology, with applications in the treatment of low back pain, knee rehabilitation, fetal movement tracking, and other fields. When strained, the resistance of the low-cost sensors is reduced, enabling human motion to be monitored using a suitable sensor array. However, current sensor technologies have exhibited significant drift, in the form of increased electrical resistance, if left stored in typical room conditions. The purpose of the present work was to evaluate the influence of several environmental factors, including temperature, humidity, oxygen levels, and light exposure, that could impact the change in electrical properties of these sensors. These physiological conditions are present during use of the sensors on human subjects as well as during sensor storage, making it vital to understand their effects on sensor properties. The electromechanical performance of the sensors stored under a range of conditions was monitored over a period of several weeks. The observations obtained indicate that the presence of oxygen and humidity in the environment where the sensors are stored is the primary contributor to drift in the sensor response. Sensors that are kept in de-oxygenated or desiccated environments do not display an increase in electrical resistance over time. This understanding allows for long-term storage of the sensors without degradation. It also assists in identifying the internal processes at work within the nanoparticle-polymer matrix that cause changes in electrical properties.</p>\u0000 </div>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142883799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of Antibiotic-Releasing Biodegradable Composite Bone Cements for Treating Experimental Chronic Maxillofacial Bone Infection","authors":"Surajit Mistry,&nbsp;Subhasis Roy,&nbsp;Debarati Bhowmick,&nbsp;Shuvojit Mistry,&nbsp;Jui Chakraborty,&nbsp;Rajiv Roy,&nbsp;Indrasri Das","doi":"10.1002/jbm.a.37858","DOIUrl":"10.1002/jbm.a.37858","url":null,"abstract":"<div>\u0000 \u0000 <p>Chronic osteomyelitis of the maxillofacial bones (i.e., jaw bones) is a persistent infection that requires effective treatment. Because systemic antibiotics seldom reach necrotic areas to remove bone infection, local antibiotic carriers such as antibiotic-loaded bone cement can be tried. It is critical to assess the biosafety and efficacy of two new antibiotic-loaded biodegradable composite bone cements for treating chronic mandibular osteomyelitis, and their drug eluting efficiency and other relevant aspects prior to clinical trial. The physico-mechanical properties, and drug release capacity of the cements were determined to be suitable for in vivo application. After inducing chronic osteomyelitis with Staphylococcal strains in 30 female rabbit mandibles, bioactive glass composite cement (0.5 g) and biphasic calcium phosphate composite cement (0.5 g) were implanted in 18 defects (nine/test group) for 84 days to compare the therapeutic efficacy with traditional therapy (control, debridement plus antibiotics in nine defects) using microscopic, micrographic, and radiological examination. Antibiotic concentrations in bone (vancomycin: 34.7–53.2 μg/g, tobramycin: 2.1–2.87 μg/g) after 21 days of installation for both cements were sufficient to eradicate pathogens without causing adverse events. In vivo tests suggest that cement groups outperformed (<i>p</i> &lt; 0.05) traditional therapy in terms of infection clearance and osteoconduction. The gross histologic and micrographic scores of biphasic calcium phosphate composite cement (10.33 ± 0.58 and 8.33 ± 1.53, respectively) indicated that the cement barely surpassed (<i>p</i> &gt; 0.05) the other composite cement (12.67 ± 1.53 and 10.0 ± 1.0, respectively). These findings emphasize the potential of antibiotic loaded composite cements as an effective treatment option for chronic maxillofacial osteomyelitis, offering a safer and more efficient alternative to traditional therapy.</p>\u0000 </div>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142886342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating the Potential of Magnesium Microparticles on Cartilage and Bone Regeneration Utilizing an In Vitro Osteoarthritis Model","authors":"Bavya Mavila Chathoth,&nbsp;Heike Helmholz,&nbsp;Nina Angrisani,&nbsp;Björn Wiese,&nbsp;Janin Reifenrath,&nbsp;Regine Willumeit-Römer","doi":"10.1002/jbm.a.37862","DOIUrl":"10.1002/jbm.a.37862","url":null,"abstract":"<p>Osteoarthritis (OA) is a significant condition that profoundly impacts synovial joints, including cartilage and subchondral bone plate. Biomaterials that can impede OA progression are a promising alternative or supplement to anti-inflammatory and surgical interventions. Magnesium (Mg) alloys known for bone regeneration potential were assessed in the form of Mg microparticles regarding their impact on tissue regeneration and prevention of OA progression. In vitro assays based on mesenchymal stem cells (SCP-1) were applied to evaluate the Mg microparticle's compatibility and function. Biocompatibility documented through live-dead staining and lactate dehydrogenase assay revealed a 90% cell viability at a concentration below 10 mM after 3 days of exposure. An in vitro OA model based on the supplementation of the cytokines IL-1β, and TNF-α was established and disclosed the effect of Mg degradation products in differentiating SCP-1 cells. Sustained differentiation was confirmed through extracellular matrix staining and increased gene marker expression. The Mg supplementation reduced the release of inflammatory cytokines (IL-6 and IL-8) while promoting the expression of proteins such as collagen X, collagen I, and osteopontin in a time-dependent manner. The in vitro study suggests that Mg microparticles hold a therapeutic potential for OA treatment with their ability to support bone and cartilage repair mechanisms even under inflammatory conditions.</p>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.a.37862","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142886327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual-Polymer Carboxymethyl Cellulose and Poly(Ethylene Oxide)-Based Gels for the Prevention of Postsurgical Adhesions","authors":"Anisha A. DSouza,&nbsp;Mansoor M. Amiji","doi":"10.1002/jbm.a.37852","DOIUrl":"10.1002/jbm.a.37852","url":null,"abstract":"<div>\u0000 \u0000 <p>Postsurgical adhesions are a common complication associated with surgical procedures; they not only impact the patient's well-being but also impose a financial burden due to medical expenses required for reoperative surgeries or adhesiolysis. Adhesions can range from a filmy, fibrinous, or fibrous vascular band to a cohesive attachment, and they can form in diverse anatomical locations such as the peritoneum, pericardium, endometrium, tendons, synovium, and epidural and pleural spaces. Numerous strategies have been explored to minimize the occurrence of postsurgical adhesions. These strategies include surgical approaches, adhesiolysis, antiadhesive agents, and mechanical barriers which have demonstrated the most promise in terms of efficacy and breadth of indications. In this review, we discuss the use of physical/mechanical barriers for adhesion prevention and outline the most commonly used, commercially available barriers. We then focus on a synthetic, dual-polymer gel composed of carboxymethyl cellulose (CMC) and poly(ethylene oxide) [PEO], which, unlike the more commonly used single-polymer hydrogels, has demonstrated higher efficacy across a greater range of indications and surgical procedures. We review the formulation, mechanical properties, and mechanisms of action of the CMC + PEO dual-polymer gel and summarize findings from clinical studies that have assessed the efficacy of CMC + PEO gels in multiple surgical settings in clinics across the world. In conclusion, the CMC + PEO dual-polymer gel represents an approach to preventing postsurgical adhesions that has been commonly used over the last 20 years and could therefore serve as a foundation for research into improving postsurgical outcomes as well as a drug delivery device to expand the use of gels in surgical settings.</p>\u0000 </div>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142886323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nitrided Ti-6Al-4V: A Catalyst for Increase Mineralization and Osteogenic Marker Expression","authors":"Annop Krasaesin,&nbsp;Suttiporn Pinijsuwan,&nbsp;Chatdanai Boonruang,&nbsp;Kanokwan Sriwattanapong,&nbsp;Thantrira Porntaveetus,&nbsp;Thanaphum Osathanon,&nbsp;Shuichi Watanabe,&nbsp;Chavin Jongwannasiri,&nbsp;Chawan Manaspon","doi":"10.1002/jbm.a.37853","DOIUrl":"10.1002/jbm.a.37853","url":null,"abstract":"<div>\u0000 \u0000 <p>Plasma nitriding is one of the surface modifications that show more effectiveness than other methods. In this study, the plasma-based ion implantation (PBII) technique was performed on the surface of titanium alloy (Ti-6Al-4V, Ti64) using a mixture of nitrogen (N₂) and argon (Ar), resulting in a plasma-nitrided surface (TiN-Ti64). The surface composition of the TiN-Ti64 was verified through X-ray photoelectron spectroscopy (XPS). TiN-Ti64 demonstrated superior hydrophilicity compared with Ti64. TiN-Ti64 exhibited higher surface hardness than the original surface. The biological responses of primary human alveolar bone cells (hAVs) were observed on the TiN-Ti64, revealing greater activation of cell adhesion and spreading compared with Ti64 and the control group (glass coverslip). Moreover, the TiN-Ti64 significantly promoted cell proliferation compared with Ti64 and tissue culture plates. The mineralization of hAVs on the TiN-Ti64 showed a significant increase, almost 20% greater than that of Ti64. Furthermore, a significant upregulation of mRNA expression for osteogenic differentiation marker genes, including <i>BMP2</i>, <i>OCN</i>, <i>OPN</i>, and <i>RUNX2</i>, was observed in TiN-Ti64 compared with other conditions. In addition, the TiN-Ti64 exhibited antibiofilm activity against <i>Streptococcus aureus.</i> In conclusion, the TiN-Ti64, modified with the PBII technique utilizing a mixture of N₂ and Ar, emerges as a promising alternative for surface modification in dental implant applications.</p>\u0000 </div>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2024-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142873706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Soluble Proteins From Conventional and Organic Eggshell Membranes With Different Proteomic Profiles Show Similar In Vitro Biofunctions","authors":"Qianli Ma,&nbsp;Lya Piaia,&nbsp;Dagnija Loca,&nbsp;Kristaps Rubenis,&nbsp;Janis Locs,&nbsp;Bernd Thiede,&nbsp;Ólafur Eysteinn Sigurjónsson,&nbsp;Håvard Jostein Haugen","doi":"10.1002/jbm.a.37848","DOIUrl":"10.1002/jbm.a.37848","url":null,"abstract":"<p>The eggshell membrane (ESM), resembling the extracellular matrix (ECM), acts as a protective barrier against bacterial invasion and offers various biofunctions due to its porous structure and protein-rich composition, such as ovalbumin, ovotransferrin, collagen, soluble protein, and antimicrobial proteins. However, the structure of ESM primarily comprises disulfide bonds and heterochains, which poses a challenge for protein solubilization/extraction. Therefore, the method of dissolving and extracting bioactive protein components from ESM has significant potential value and importance for exploring the reuse of egg waste and environmental protection. In this study, soluble ESM proteins (SEPs) were extracted from conventional (industrial-fed) and organic (free-grounded) using an acidic 3-mercaptopropionic acid (3-MPA) extraction strategy. FTIR was employed to monitor the chemical changes in the ESM, while LC–MS/MS was used to conduct the proteomic analysis. The biocompatibility and effects of SEP cocktails on ECM synthesis were also investigated. The results indicated that the acidic 3-MPA strategy effectively altered the ESM chemical composition, thereby facilitating SEPs extraction. The SEPs from conventional and organic eggs have different protein profiles but with partial overlapping. SEPs from both sources showed similar desirable biosafety profiles and dose-dependent promotion of osteoblastic (ECM) component synthesis, suggesting that different egg sources may contribute to consistent core biological functions of protein products, they may also introduce different functional priorities.</p>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.a.37848","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142848463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}