Journal of biomedical materials research. Part A最新文献

{"title":"Coaxial Bioprinting of Schwann Cells and Neural Stem Cells in a Three-Dimensional Microenvironment for the Repair of Peripheral Nerve Defects","authors":"Xuanzhi Wang,&nbsp;Tao Xu,&nbsp;Fei Wang","doi":"10.1002/jbm.a.37943","DOIUrl":"https://doi.org/10.1002/jbm.a.37943","url":null,"abstract":"<div>\u0000 \u0000 <p>Currently, autologous nerve (AN) transplantation remains the gold standard for treating peripheral nerve injuries (PNIs). However, its inherent limitations, including donor site morbidity and immune rejection risks associated with allografts, have prompted the exploration of alternative therapeutic strategies. Among these, tissue engineering approaches have gained significant attention, with nerve conduit design emerging as a particularly promising research direction. Electrospinning technology has been widely adopted for its ability to fabricate nanofibrous scaffolds that closely mimic the native extracellular matrix. In this study, we engineered an aligned nanofiber conduit utilizing polylactic acid and gelatin through electrospinning, and integrated a sodium alginate hydrogel enriched with Schwann cells (SCs) and neural stem cells (NSCs) via coaxial bioprinting. The three-dimensional (3D) hydrogel microenvironment facilitated synergistic interactions between SCs and NSCs, augmenting the secretion of neurotrophic factors such as brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF). A dynamic perfusion culture system was further employed to optimize cell viability and functionality. In vivo studies revealed that the implantation of this conduit in a sciatic nerve defect model markedly enhanced motor function recovery, nerve regeneration, and muscle morphology. These improvements were substantiated by an increased sciatic functional index (SFI), heightened expression of S-100 and NF-200, and greater myelin thickness and axon diameter. Although the efficacy of the 3D-aligned nanofiber conduit cocultured with SCs and NSCs approximated that of AN transplantation, further research is imperative to identify more efficient seed cells and biocompatible 3D carriers to achieve optimal nerve regeneration. This study highlights the potential of tissue-engineered nerve conduits as a viable alternative for PNI repair, paving the way for future advancements in the field.</p>\u0000 </div>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 7","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144367421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing the Potency of Growth Factor-Mimicking Peptides via Cross-Presentation With Integrin Ligands","authors":"Sydney Neal,&nbsp;Xiaohong Tan,&nbsp;Era Jain,&nbsp;Charlotte Chen,&nbsp;Mohammadjafar Hashemi,&nbsp;Lori A. Setton,&nbsp;Nathaniel Huebsch","doi":"10.1002/jbm.a.37944","DOIUrl":"https://doi.org/10.1002/jbm.a.37944","url":null,"abstract":"<p>Growth factors enhance survival and integration of transplanted Mesenchymal Stromal Cells (MSC), but successful supplementation often requires supraphysiological growth factor doses, risking off-target effects. Short peptide mimics like the knuckle epitope (KE) of Bone Morphogenetic Protein 2 (BMP-2) can be covalently immobilized to biomaterials, localizing bioactivity at the delivery site. However, these short peptides often lack the potency of full-length growth factors. We sought to improve the potency of alginate-grafted KE to encourage MSC osteogenic differentiation. When alginate gels co-presented KE and integrin-binding cyclo-RGD (cRGD) peptides, MSC expressed early markers of osteogenesis (Runt-related Transcription Factor2, RUNX2, Alkaline Phosphatase, ALP, and osteocalcin, OCN) in a KE-dose dependent manner. When co-presented with cRGD, high concentrations of KE partially mimicked the osteogenic potential (ALP induction) of full-length BMP-2. Proximity between KE and cRGD may be the mechanism through which high dose KE induces osteogenesis in the presence of cRGD. To investigate this possibility, we used orthogonal strain-promoted azide-alkyne cycloaddition (SPAAC) and maleimide-thiol chemistries to graft KE and cRGD in a bivalent (same alginate chain) and a monovalent (different alginate chain) manner, at constant bulk peptide concentration. Bivalent presentation of peptides (separation distance of 5.5 ± 0.5 nm verified by FRET) ultimately increased RUNX2 and ALP expression compared to monovalent presentation. This platform technology can be used in future studies to control peptide nanopatterning to enhance potency, in the context of MSC-based therapies and beyond.</p>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 7","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.a.37944","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144367420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to “Injectable Hydrogel Scaffold Incorporating Microspheres Containing Cobalt-Doped Bioactive Glass for Bone Healing”","authors":"","doi":"10.1002/jbm.a.37910","DOIUrl":"https://doi.org/10.1002/jbm.a.37910","url":null,"abstract":"<p>\u0000 <span>Ghiasi Tabari, P</span>, <span>Sattari, A</span>, <span>Mashhadi Keshtiban, M</span>, <span>Karkuki Osguei, N</span>, <span>Hardy, JG</span>, <span>Samadikuchaksaraei, A.</span> <span>Injectable Hydrogel Scaffold Incorporating Microspheres Containing Cobalt-Doped Bioactive Glass for Bone Healing</span>. <i>J Biomed Mater Res A.</i> <span>2024</span>, <span>112</span>(<span>12</span>): <span>2225</span>–<span>2242</span>; doi: https://doi.org/10.1002/jbm.a.37773.\u0000 </p><p>In Figure 4, panel A-S-G7Co of Alizarin Red S staining in our original article, an incorrect figure was used. Figure 4 is now updated with new correct panel for A-S-G7Co of Alizarin Red S staining. Please note that this correction does not affect the results, the description and interpretation of the results, or the conclusions of the article.</p><p>We apologize for this error.</p>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 7","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.a.37910","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144367422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrochemical Assessment of the Galvanic Corrosion and Metal Ion Release in Overlapping Stent and Vascular Plug Systems","authors":"O. Burak Istanbullu,&nbsp;Gulsen Akdogan,&nbsp;Halis Yilmaz,&nbsp;Mustafa Istanbullu","doi":"10.1002/jbm.a.37946","DOIUrl":"https://doi.org/10.1002/jbm.a.37946","url":null,"abstract":"<p>Cardiovascular diseases cause the highest global mortality rates and are often treated with surgical interventions such as stent or vascular plug placement. However, in-stent restenosis develops over time depending on the material composition and interactions with body fluids. Current strategies to address restenosis include balloon angioplasty or placing a secondary stent at the same site. A key concern with overlapping stents is the increasing risk of galvanic corrosion, as most cardiovascular stents have metallic composition. This study examines galvanic corrosion rates in different vascular stent and plug combinations using electrochemical corrosion characterization techniques. Three metallic vascular specimens with varying compositions are evaluated. The specimens are immersed in simulated body fluid at 37°C under individual and overlapping conditions. Electrochemical impedance spectroscopy and current density measurements, conducted via potentiostat, provide insights into the corrosion behavior of each specimen configuration. Additionally, inductively coupled plasma mass spectrometry quantifies metal ion release through SBF samples. Results show that combining dissimilar materials in overlapping placements significantly increases galvanic corrosion and metal ion release. The corrosion current density (<i>i</i>_corr) significantly increased from 11.75 μA/cm² in the individual bare-metallic stent to 522.3 μA/cm² in the stent-on-plug configuration. A similar increase was observed in the stent-on-stent configuration, with an <i>i</i>_corr of 132.6 μA/cm². These results corresponded with notable decreases in electrochemical impedance and polarization resistance, measured as low as 0.039 kΩ (<i>Z</i>_T) and 0.057 kΩ cm² (<i>R</i>_P) for the stent-on-plug system. Consequently, the calculated corrosion rate escalated to 2254 μm/year, with a mass loss reaching 42.22 mg/cm²·year. ICP-MS analysis supported these findings, showing the highest levels of metal ion release in the stent-on-plug configuration, with 23.86 ppm of Ni and 0.41 ppm of Cr. These findings highlight the importance of stent-material selection in reducing corrosion-related complications. Implementing material-specific strategies in secondary stent placement can lower the risks of inflammatory host response, stent failure, and their long-term effects.</p>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 6","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.a.37946","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144292931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tendon-Tissue Derived Monofilaments by Electrochemical Compaction: Production and Characterization","authors":"Phillip McClellan,&nbsp;Joohee Choi,&nbsp;Mikhail Nasrallah,&nbsp;Kathleen Lundberg,&nbsp;Ozan Akkus","doi":"10.1002/jbm.a.37948","DOIUrl":"https://doi.org/10.1002/jbm.a.37948","url":null,"abstract":"<p>Repair of tendon tissues remains a complex problem in orthopedic surgery. Tendon auto- and allografts are not utilized to the full extent of their capabilities due largely to the lack of porosity and availability of properly processed tendon stock. Cryomilling is often utilized to maximize surface area-to-volume while limiting alterations to native protein/gene structure. In this study, native tendons were isolated, cryomilled, and decellularized using a truncated protocol. The resulting decellularized tendon powder exhibited reduced DNA content of less than 15 ng/mg, indicating effective removal of cellular components. The resulting decellularized tendon “powder” was then subjected to mild acidic conditions to partially solubilize the collagen within the extracellular matrix to produce a solution that could be electrochemically compacted to generate aligned fibers. Proteomic analyses revealed the presence of tendon-related proteins (cartilage oligomeric protein, fibromodulin, lumican, biglycan, and tenascin c). Proteoglycans were present in tendon-derived thread (TDT) and largely absent in pure collagen threads, as visualized by safranin O and quantified by dimethylmethylene blue staining. Mesenchymal stem cells seeded and cultured for up to 14 days on collagen threads and TDTs exhibited similar expression of genes related to tendon tissue.</p>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 6","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.a.37948","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144299867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the Reactions Induced by Bioactive Glass Air Abrasion of Titanium and Their Effects on Osteoblast Cellular Responses","authors":"Faleh Abushahba,&nbsp;Adrian Stiller,&nbsp;Sherif A. Mohamad,&nbsp;Nagat Areid,&nbsp;Leena Hupa,&nbsp;Terhi J. Heino,&nbsp;Pekka K. Vallittu,&nbsp;Timo O. Närhi","doi":"10.1002/jbm.a.37949","DOIUrl":"https://doi.org/10.1002/jbm.a.37949","url":null,"abstract":"<p>This study investigated the chemical events that occur when titanium (Ti) surfaces are treated with air particle abrasion (APA) using zinc-containing bioactive glass (ZnBG), followed by immersion in simulated body fluid (SBF) for up to 96 h. The impact of these changes on osteoblast cell viability, adhesion, and differentiation was evaluated. Sandblasted and acid-etched (SA) Ti disks were subjected to APA with ZnBG particles and then immersed in SBF from 8 to 96 h. Ion dissolution and characterization of ZnBG powder and Ti disks were conducted. Analyses of osteoblast viability, adhesion, and alkaline phosphatase (ALP) activity were performed on MC3T3-E1 cells cultured on control disks (SA-Ti), as well as on ZnBG abraded disks (APA-Ti) and disks immersed for 96 h in SBF (CaP-Ti). After SBF immersion, the ZnBG particle surfaces showed a rise in Si atomic (at.)% within the first 8 h, while Ca remained stable, and the P doubled over 96 h. The ZnBG covering the disks dissolved during the first 8 h, and then the Ca, P, and Si at.% increased as the immersion time extended. The glass particles exhibited amorphous calcium–phosphate (Ca–P) layer formation after 96 h. A significantly (<i>p</i> = 0.004) higher cell viability level was observed on day 7 on APA-Ti compared to SA-Ti disks, while no differences in osteoblast differentiation were observed across the different surfaces. Fluorescence images demonstrated that on day 3, cells adhered to valleys and peaks of CaP-Ti threads but only to valleys on SA-Ti and APA-Ti disks. By day 7, cells were also observed on APA-Ti peaks but not on SA-Ti. In summary, APA enhanced osteoblast proliferation, and a biocompatible Ca–P layer, which formed upon mineralization, supported osteoblast viability, adhesion, and spreading.</p>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 6","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.a.37949","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144291899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tyrosine-Derived Polymeric Surfactants Modulate the Fusion of Normal and Cancer Cells","authors":"David I. Devore,&nbsp;Dongming Sun,&nbsp;Iman Tadmori,&nbsp;Kim-phuong N. Le,&nbsp;Mariana R. N. Lima,&nbsp;Joachim Kohn","doi":"10.1002/jbm.a.37941","DOIUrl":"https://doi.org/10.1002/jbm.a.37941","url":null,"abstract":"<p>Plasma membrane fusion and resealing play essential roles in diverse biological processes, including embryogenesis, morphogenesis, tissue repair, and cancer metastasis. Certain polymeric surfactants, including poly(ethylene glycol) (PEG) and triblock poly(alkylene oxides) like Poloxamer 188 (P188), are known to modify cell membrane biophysical properties. This has enabled applications such as PEG fusion for severed nerves and P188-mediated muscle tissue repair. Similar to P188, tyrosine-derived triblock copolymers (TyPS) form self-assembled nanospheres that can reversibly insert into phospholipid monolayers and cell plasma membranes. The effects of phospholipid head group polarity on the insertion of TyPS into Langmuir phospholipid monolayers are examined here. The hydrophobic blocks of the polymeric surfactants are found to provide the primary driving force for insertion in the phospholipid membranes. The impact of the TyPS, PEG, and P188, alone and in combination, on membrane fusion in normal (L929 mouse fibroblast) and transformed (MDA-MB-231 human breast cancer) cells is then determined using in vitro cell culture methods. The cell culture studies demonstrate that PEG induces fusion in both cell lines and reveal that the combination of PEG and P188 has a strong positive synergistic effect on cell fusion. In contrast, the TyPS exhibits strong anti-fusion properties, inhibiting both spontaneous and PEG-enhanced fusion. P188 has a weak antifusion effect compared to TyPS. The fusogenic or antifusogenic behaviors of the polymeric surfactants correlate with their thermodynamic Hansen solubility parameters, and the synthetic tunability of the TyPS enables access to a far greater range of hydrophobicities than the available commercial Poloxamers. These findings suggest that mixtures of PEG and P188 may have the potential to enhance tissue repair and hybridoma output for monoclonal antibody production, while the TyPS may have the potential to inhibit metastatic cancers.</p>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 6","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.a.37941","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144292930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation and Characterization of AgNWs Conductive Hydrogel With High Mechanical Performance, High Electrical Conductivity, and Biocompatibility","authors":"Shui Guan,&nbsp;Fengxu Wang,&nbsp;Chuzhou Wen,&nbsp;Hailong Liu,&nbsp;Changkai Sun","doi":"10.1002/jbm.a.37951","DOIUrl":"https://doi.org/10.1002/jbm.a.37951","url":null,"abstract":"<div>\u0000 \u0000 <p>Hydrogel scaffolds incorporating conductive fillers have garnered significant interest due to their potential applications in neural tissue repair and regenerative medicine. However, most conductive fillers have adverse effects on the mechanical properties of hydrogel networks. In the present study, a novel polyacrylamide/alginate (PAAm/Alg) assembled with conductive silver nanowires (AgNWs) composite hydrogel was developed through photopolymerization and crosslinking methods. The chemical structure, morphology, mechanical properties, conductivity, porosity, swelling rate, adhesive strength, thermal stability, in vitro biodegradation, and biocompatibility of the prepared hydrogel samples were investigated. The PAAm/Alg-AgNWs hydrogels exhibited uniform pore structure distribution, high porosity and water absorption, improved mechanical and conductive properties, good thermal stability, and adequate biodegradability. In particular, the 0.4 wt% AgNWs conductive hydrogel exhibited excellent conductivity of 0.618 S/m and a high Young's modulus of 43.6 kPa, along with good electrical durability and stability over ten cyclic loading. Moreover, the abundant hydrophilic groups in the hydrogel make it have good adhesion properties at different interfaces. Compared with the PAAm/Alg hydrogel, the incorporation of AgNWs enhanced the material's roughness, facilitating cell adhesion, viability, and proliferation. These results showed that the AgNWs assembled into the PAAm/Alg polymers endowed the hydrogel with high electrical conductivity, while excellent mechanical strength and biocompatibility, indicating an attractive conductive substrate for further studies on neural tissue repair and regeneration.</p>\u0000 </div>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 6","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144281446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis and Evaluation of Mesoporous Silica-Biopolymer-Based Bone Substitutes for Tissue Engineering","authors":"María Inés Álvarez Echazu,&nbsp;Sandra Judith Renou,&nbsp;Christian Ezequiel Olivetti,&nbsp;Gisela Solange Alvarez,&nbsp;Martin Federico Desimone,&nbsp;Daniel Gustavo Olmedo","doi":"10.1002/jbm.a.37927","DOIUrl":"https://doi.org/10.1002/jbm.a.37927","url":null,"abstract":"<div>\u0000 \u0000 <p>Bone substitutes for tissue regeneration should provide an appropriate environment for cell attachment, differentiation, proliferation, and migration. 3D structure, degradability, swelling, porosity, and cytotoxicity have been highlighted as key points in their design. For this research, mesoporous silica-biopolymer composites were synthesized from mesoporous silica (Mes-Si) particles combined with either collagen (C/Mes-Si) or chitosan (CS/Mes-Si). The composites were evaluated for tissue engineering purposes, as bone substitutes intended to imitate features of the natural bone matrix, thereby providing an appropriate biochemical environment for bone repair. Physicochemical-biological evaluation was performed to identify the features that would be useful for bone tissue engineering. For the Mes-Si particles, the specific surface area was 750.95 m²/g and the average pore size was 3.47 nm. SEM images showed that Mes-Si particles were distributed within the chitosan (CS) or collagen (C) matrix. Both composites swelled rapidly and had low cytotoxicity. Histologically, no acute inflammatory infiltrate or giant multinucleated cell was observed 14 days after implantation. In C/Mes-Si, newly woven bone tissue and areas of osseointegration at the C/Mes-Si-tissue interface were observed. In CS/Mes-Si, only reparative granulation tissue was observed. The physicochemical properties and biocompatibility of both composites were adequate for a bone scaffold. Moreover, Mes-Si particles have a tunable surface area for chemical modifications and anchoring bioactive materials, which may enhance composite bioactivity or the delivery of bioactive materials.</p>\u0000 </div>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 6","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144273349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional Nanoparticle-Enhanced Silk Hydrogels for Tissue Engineering Biomaterials","authors":"Olivia K. Foster,&nbsp;Derek Hiscox,&nbsp;Sawnaz Shaidani,&nbsp;Jean Park,&nbsp;Ella Canas,&nbsp;Charlotte Jacobus,&nbsp;Riley Patten,&nbsp;David L. Kaplan","doi":"10.1002/jbm.a.37945","DOIUrl":"https://doi.org/10.1002/jbm.a.37945","url":null,"abstract":"<div>\u0000 \u0000 <p>Hydrogels prepared from natural polymers, such as silk fibroin, are useful in the field of tissue engineering due to their biocompatibility, biodegradability, and biological performance. However, poor mechanical properties can limit their broader utility. This study investigated reinforcing enzymatically crosslinked silk hydrogels with 130 nm silk nanoparticles (SNPs) to generate silk-silk composite materials with tunable strength and stiffness. The strength of the materials was dependent on SNP concentration, and hydrogels with Young's moduli of 14, 34, and 67 kPa were fabricated by adding no SNPs, 2 mg/mL SNPs, and 4 mg/mL SNPs, respectively. These methods were applied to silk bioinks using Freeform Reversible Embedding of Suspended Hydrogels (FRESH) 3D printing to fabricate complex 3D structures with control of elasticity and modulus. Cylinders with Young's moduli of 17, 35, and 58 kPa were obtained with no SNPs, 2 mg/mL SNPs, and 4 mg/mL SNPs, respectively. SNPs were also preloaded with epidermal growth factor (EGF), relevant for tissue development and wound healing, and sustained release was achieved for over 15 days when embedded in hydrogels. Pilot studies of dermal fibroblast encapsulation in SNP-reinforced silk hydrogels demonstrated cytocompatibility. Tunable silk hydrogels reinforced with SNPs provide application-specific scaffolding for a variety of biomaterial and tissue engineering applications.</p>\u0000 </div>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 6","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144273557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}