Journal of biomedical materials research. Part A最新文献

{"title":"Hydroxyapatite-Coated Ti6Al4V With Liposome-Encapsulated Gooseberry Extract for Orthopedic Applications","authors":"Bryson White,&nbsp;Susmita Bose","doi":"10.1002/jbm.a.70160","DOIUrl":"10.1002/jbm.a.70160","url":null,"abstract":"<p>This study investigates gooseberry (<i>Phyllanthus emblica</i>) extract (GB) as a bioactive additive to provide antibacterial activity and activity against osteosarcoma cells in hydroxyapatite-coated Ti6Al4V implants while maintaining osteoblast cytocompatibility. Aqueous extraction of GB yields a multicomponent formulation containing hydrophilic bioactive constituents that are prone to rapid release and poor retention under physiological conditions, thereby limiting their utility in implant-based delivery systems. To address this limitation, GB was encapsulated within liposomes to mitigate burst release and enable sustained delivery from implant surfaces. When incorporated onto plasma-sprayed hydroxyapatite-coated Ti6Al4V substrates, liposomal encapsulation reduced burst release within the first 48 h by 18% at pH 5.0 and 27% at pH 7.4. The formulation reduced osteosarcoma cell viability by 58% ± 7% after 11 days and decreased <i>Pseudomonas aeruginosa</i> and <i>Staphylococcus aureus</i> viability by 78% ± 3% and 45% ± 6%, respectively, within 48 h. Importantly, the system exhibited no cytotoxic effects toward osteoblasts, as demonstrated by MTT cell viability assays. In vivo evaluation of GB-treated implants after 6 weeks further demonstrated no adverse effect on early bone formation, with histological analysis showing a trend toward increased bone formation relative to control implants. These results demonstrate the feasibility of stabilizing and delivering a complex, hydrophilic natural extract from implant surfaces using liposomal encapsulation while maintaining osteoblast compatibility and without adversely affecting early bone formation in vivo.</p>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"114 10","pages":""},"PeriodicalIF":3.8,"publicationDate":"2026-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.a.70160","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149070747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Scalable Scaffold Materials for Structured Cultivated Meat","authors":"Alex S. Kermani,&nbsp;Andrea C. Filler,&nbsp;J. Kent Leach","doi":"10.1002/jbm.a.70162","DOIUrl":"https://doi.org/10.1002/jbm.a.70162","url":null,"abstract":"<div>\u0000 \u0000 <p>Cultivated meat (CM) seeks to decouple meat production from animal agriculture, but producing structured products that consumers recognize as whole-cut meat remains both difficult and central to the field's ambitions. Scaffolds are key to this goal, providing architecture, cell-adhesion sites, and instructive cues required to organize cells into texturally mimetic tissue. To date, CM scaffold research has largely borrowed from tissue engineering, which optimizes for therapeutic function and does not account for the constraints that define a food product: edibility, low cost, and large scale. Here, we review scalable scaffold materials and fabrication methods for structured CM through this food-first lens. We identify relevant elements of the native muscle microenvironment and assess how these characteristics have been recapitulated. We then evaluate the major classes of scaffold materials, including those of synthetic, animal, plant, fungal, and microbial origin, as well as waste-stream-derived materials, and compare their biological suitability and scalability. We organize fabrication methods by the biological function they address: cell expansion, alignment, bulk structure, and multi-material assembly, treating mass transport as a constraint that cuts across these methods rather than a separate function. Throughout, we emphasize that consumer perception of meat quality is driven by architecture rather than physiological function, and throughput and cost determine commercial viability. We conclude by identifying critical gaps that warrant scientific focus to bring structured CM to market.</p>\u0000 </div>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"114 10","pages":""},"PeriodicalIF":3.8,"publicationDate":"2026-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148994299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Decellularized Fish Swim Bladder Extracellular Matrix–Polycaprolactone Composite Material for Application in Small-Diameter Vascular Grafts","authors":"Yifan Wu,&nbsp;Minghao Xu,&nbsp;Xixi Wang,&nbsp;Chijia Wang,&nbsp;Zhihong Wang,&nbsp;Jing Liu","doi":"10.1002/jbm.a.70154","DOIUrl":"10.1002/jbm.a.70154","url":null,"abstract":"<div>\u0000 \u0000 <p>The high incidence of cardiovascular diseases has led to an increasing demand for small-diameter vascular grafts. Polycaprolactone (PCL) is a commonly used synthetic polymer material in the construction of vascular grafts. However, PCL alone has limited bioactivity, which may affect its blood-contacting and tissue responses when used in small-diameter vascular grafts. Fish swim bladder-derived extracellular matrix (FSB–ECM) contains bioactive components such as collagen and has been investigated as a cardiovascular biomaterial, although its mechanical properties are limited. In this study, fish swim bladder-derived dECM was incorporated into PCL by electrospinning to fabricate a series of small-diameter vascular grafts. The study first confirmed that dECM was successfully loaded onto PCL fibers and significantly improved the hydrophilicity and degradation performance of the material. Based on the in vitro and ex vivo evaluations, the 15% PCL–dECM (3:7) formulation was selected for further in vivo testing because it showed a favorable balance of mechanical properties and hemocompatibility, including low platelet activation. Subsequent in vivo evaluation in a rat abdominal artery implantation model demonstrated that this dECM-modified graft achieved a 100% patency rate. Furthermore, it exhibited significant anti-inflammatory effects and was conducive to the organized deposition of extracellular matrix components, such as collagen and elastic fibers. This work successfully developed a novel small-diameter vascular graft that possesses both excellent bioactivity and mechanical properties. Our findings offer a promising strategy and a solid experimental foundation, supporting the future clinical translation of small-diameter vascular grafts.</p>\u0000 </div>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"114 10","pages":""},"PeriodicalIF":3.8,"publicationDate":"2026-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148993294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Annexin V-Functionalized Injectable Calcium-Deficient Hydroxyapatite for Extracellular Vesicles Binding and Controlled Release","authors":"Eda Ciftci,&nbsp;Sefa Burak Cam,&nbsp;Feza Korkusuz,&nbsp;Emre Erdem,&nbsp;Feray Bakan Misirlioglu,&nbsp;Petek Korkusuz","doi":"10.1002/jbm.a.70159","DOIUrl":"https://doi.org/10.1002/jbm.a.70159","url":null,"abstract":"<p>Calcium phosphates alone have a limited induction potential in challenging bone conditions, necessitating strategies that enhance cellular responses and matrix-related processes. An injectable formulation combining calcium-deficient hydroxyapatite (CDHA) with mesenchymal stem cell-derived extracellular vesicles may present a promising approach for modulating osteogenic activity. A dual formulation composed of calcium-deficient HA and human bone marrow mesenchymal stem cell-derived extracellular vesicles, incorporating Annexin V as a binding molecule, was generated and validated. Structural and molecular characterization was performed using Raman spectroscopy, electron paramagnetic resonance, and sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Real-time impedance-based cell proliferation analysis was applied to determine biologically effective concentrations. The effects of the formulation on human osteoblast behavior and early osteogenic activity were additionally evaluated. Raman spectroscopy confirmed structural integration within the formulation termed CHAnEx, showing peak shifts and spectral features at 959, 630, and 806 cm⁻¹, indicating molecular interactions between calcium-deficient HA, Annexin V, and extracellular vesicles. Electron paramagnetic resonance (EPR) analysis revealed distinct g-factor shifts consistent with binding interactions. Real-time proliferation analysis identified biologically effective concentrations of calcium-deficient HA and extracellular vesicles using human osteoblast models. The combined formulation increased alkaline phosphatase activity, showing approximately a 20%–25% increase compared to control groups. These findings indicate that the calcium-deficient HA-extracellular vesicle formulation supports functional interactions and modulates osteoblast responses under in vitro conditions, suggesting its relevance as an injectable biomaterial platform.</p>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"114 10","pages":""},"PeriodicalIF":3.8,"publicationDate":"2026-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.a.70159","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148974934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D-Printed Ordered Porous CSMA/GelMA Hydrogel Scaffolds Enhance ADSC Reparative Function With FAK–YAP-Related Mechanotransductive Changes","authors":"Songlu Tseng,&nbsp;Jieyu Xiang,&nbsp;Ziming Li,&nbsp;Lin Kang,&nbsp;Liquan Wang,&nbsp;Chi Zhang,&nbsp;Zhujun Li,&nbsp;Nanze Yu,&nbsp;Jiuzuo Huang,&nbsp;Xiao Long","doi":"10.1002/jbm.a.70145","DOIUrl":"10.1002/jbm.a.70145","url":null,"abstract":"<div>\u0000 \u0000 <p>Chronic diabetic wounds persist in a hostile microenvironment that compromises the survival and reparative activity of transplanted stem cells. Here, we developed a structurally defined 3D-printed ordered porous chitosan methacrylate/gelatin methacryloyl (CSMA/GelMA) hydrogel scaffold platform to test whether topological order acts as a structurally relevant biomaterial cue for adipose-derived mesenchymal stem cell (ADSC) regulation. To enrich for topology-dependent effects, ordered and disordered scaffolds were fabricated from the same photocrosslinkable chitosan methacrylate/gelatin methacryloyl precursor system and subjected to the same crosslinking and lyophilization–rehydration workflow, while differing in spatial pore organization. Among the ordered architectures tested, the 200 μm scaffold showed the most favorable early biological performance and was selected for subsequent studies. Ordered topology induced ADSC polarization, directional F-actin organization, and nuclear elongation/alignment, indicating coordinated cytoskeletal–nuclear remodeling. Mechanistically, ordered scaffolds increased FAK phosphorylation and enhanced YAP nuclear localization, whereas pharmacological inhibition of FAK attenuated YAP-related responses and the reparative paracrine advantage conferred by ordered topology. On this basis, sustained SDF-1α presentation further enhanced the reparative secretory profile, at least partly through CXCR4-AKT/ERK-associated signaling. In an STZ-induced diabetic full-thickness skin wound model, the integrated ordered scaffold strategy improved wound closure, histological reconstruction, collagen deposition, and CD31- and CD206-associated reparative changes, with the greatest benefit observed when combined with ADSCs. These findings support a topology–mechanotransduction–function axis within this scaffold system and provide a rationale for biomaterial-guided stem-cell therapy in diabetic wound healing.</p>\u0000 </div>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"114 9","pages":""},"PeriodicalIF":3.8,"publicationDate":"2026-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148922122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioluminescent Trace Labeling of Porous Protein Microcrystals for Evaluating Their Pharmacodynamics","authors":"Alec Jones,&nbsp;Anika O'Brian,&nbsp;Isaac Lewis,&nbsp;Cole Shepherd,&nbsp;Tim Ahr,&nbsp;Lauren Beatty,&nbsp;Lauren Frueh,&nbsp;Matthieu Henry,&nbsp;Laura Chubb,&nbsp;Christopher D. Snow,&nbsp;Katherine E. Horak","doi":"10.1002/jbm.a.70127","DOIUrl":"10.1002/jbm.a.70127","url":null,"abstract":"<div>\u0000 \u0000 <p>In this exploratory pilot study, we evaluated bioluminescent trace labeling as a strategy for monitoring the in vivo distribution of cross-linked porous protein microcrystals grown from the CJ protein. CJ crystals doped with CJ-SpyTag003 were functionalized with SpyCatcher003-NanoLuciferase and imaged after intravenous administration in BALB/c mice. In vitro experiments confirmed that SpyCatcher-fusion proteins could be retained by CJ-SpyTag003 crystals and that NanoLuciferase remained active after association with the crystal scaffold. Following tail-vein injection, whole-animal IVIS imaging revealed early thoracic signal consistent with first-pass pulmonary exposure, followed by rapid signal decay. A subset of animals showed persistent hindlimb-associated signal, but terminal ex vivo imaging at 72 h showed no statistically significant organ-level differences after sensitivity analysis excluding one extreme muscle-associated value. These results demonstrate the feasibility of NanoLuciferase-based trace labeling for longitudinal imaging of CJ microcrystals while highlighting important interpretive limitations of substrate-dependent bioluminescence, including furimazine delivery, local substrate pooling, reporter stability, and the inability of bioluminescence alone to distinguish intact crystals from released or surface-associated NanoLuciferase. Overall, this work provides an initial in vivo imaging framework for CJ protein microcrystals and identifies particle size, reporter stability, and orthogonal tracking as priorities for future pharmacokinetic studies.</p>\u0000 </div>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"114 9","pages":""},"PeriodicalIF":3.8,"publicationDate":"2026-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148914640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Innovative In Vivo Approaches to Bone Regeneration: Combining Chitosan-Xanthan Gum Membranes, Electrical Stimulation, and Laser Therapy","authors":"Julia Eduarda Schneider Gregório,&nbsp;Renata Francielle Bombaldi de Souza,&nbsp;Maisa Campagnollo,&nbsp;Gláucia Maria Tech dos Santos,&nbsp;Fernanda Carla Bombaldi de Souza,&nbsp;Ângela Maria Moraes,&nbsp;Milton Santamaria-Jr,&nbsp;Guilherme Ferreira Caetano","doi":"10.1002/jbm.a.70152","DOIUrl":"10.1002/jbm.a.70152","url":null,"abstract":"<p>Critical bone defects present significant clinical challenges due to their debilitating nature and associated costs. Tissue engineering offers a promising solution, employing biomaterials like porous chitosan (CH) and xanthan gum (XG) membranes as scaffolds for tissue regeneration. Electrical stimulation (ES) and laser (L) therapy, known for osteogenic and anti-inflammatory effects, respectively, may synergistically enhance bone healing when combined with these membranes. This study evaluated CH/XG membranes and their association with ES and L for the treatment of critical bone defects (25 mm²) created in 80 male Wistar rats. The animals were divided into four groups: untreated, CH/XG, CH/XG + ES, and CH/XG + L, assessed at days 30 and 90. Histomorphometric and real-time PCR analyses revealed greater tissue and blood vessel formation in the CH/XG, CH/XG + ES, and CH/XG + L groups compared to the untreated group. At day 30, reduced <i>Runx-2</i> and <i>Osx</i> expression in the treated groups suggested an initial high inflammatory response. The CH/XG group showed pronounced inflammation (elevated <i>Il-1β</i> and pro-inflammatory markers) alongside an anti-inflammatory response. Conversely, compared with the CH/XG-only group, the CH/XG + ES and CH/XG + L groups generally showed lower expression of the evaluated pro-inflammatory markers after 30 days, together with treatment- and time-dependent changes in anti-inflammatory markers. Although inflammation limited the initial effectiveness of CH/XG membranes, significant positive impacts were observed after 90 days. Combining membranes to cover bone defects and adjunctive therapies, as demonstrated in this study, emerges as a beneficial approach for enhancing bone repair outcomes. Adding osteoconductive or osteoinductive fillers may bring further healing improvements.</p>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"114 9","pages":""},"PeriodicalIF":3.8,"publicationDate":"2026-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.a.70152","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148908326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antioxidant-Thermoresponsive Collagen/Chitosan Nanocomposite Hydrogels With Praseodymium-Doped Mesoporous Bioglass for Enhanced Bone Regeneration","authors":"Kulwinder Kaur,&nbsp;Zhiyan Xu,&nbsp;Aldo. R. Boccaccini,&nbsp;Ciara M. Murphy","doi":"10.1002/jbm.a.70128","DOIUrl":"https://doi.org/10.1002/jbm.a.70128","url":null,"abstract":"<p>Oxidative stress is a critical barrier to effective bone regeneration, often impairing cellular function and osteogenic differentiation. In this study, we report the novel development of a multifunctional chitosan/collagen (CS/Coll) hydrogel integrated with praseodymium-doped mesoporous bioactive glass nanoparticles (Pr-MBGNs) engineered for the first time to simultaneously scavenge reactive oxygen species (ROS) and promote osteogenesis. The antioxidant potential of the Pr-MBGNs-loaded hydrogels was confirmed through hydrogen peroxide (H₂O₂) scavenging assays, demonstrating over 98% H₂O₂ reduction at optimal concentrations with significant reduction in intracellular ROS production. Under oxidative stress conditions, the hydrogels significantly restored rMSC viability, enhanced cell adhesion, and supported sustained proliferation. Mechanically, the incorporation of Pr-MBGNs modulated hydrogel crosslink density and stiffness, which favor improved cell–material interactions. Osteogenic assays showed a significant upregulation of key osteogenic markers (RUNX2, OCN, and OSX) and increase in matrix mineralization over 28 days. These outcomes are attributed to the synergistic effect of antioxidant protection and sustained release of bioactive ions from the Pr-MBGNs, which reprogrammed the redox environment and enhanced the osteoinductive microenvironment. Collectively, this study presents a first-of-its-kind nanoengineered hydrogel system that integrates ROS scavenging and osteogenic stimulation, offering a promising biomimetic strategy for bone tissue engineering in oxidative stress-related pathological conditions.</p>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"114 9","pages":""},"PeriodicalIF":3.8,"publicationDate":"2026-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.a.70128","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148878313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Profiling the Glycosignature of Commercial Pepsin-Soluble Type I Collagen Preparations: A MultiModal Lectin Strategy Reveals Latent Batch Variability","authors":"Mohamed EL-Melegy,&nbsp;Amir M. Alsharabasy,&nbsp;Abhay Pandit","doi":"10.1002/jbm.a.70148","DOIUrl":"https://doi.org/10.1002/jbm.a.70148","url":null,"abstract":"<p>Type I collagen (Col-I) is widely used in biomaterials and cell biology, yet the glycosignature of commercial Col-I preparations remains poorly characterized. Here, we established an integrated lectin-based workflow to profile the glycan landscape of commercially sourced bovine pepsin-soluble Col-I as supplied for biomaterial use. Using a panel of 15 biotinylated and fluorescent lectins, we combined qualitative fluorescence staining, quantitative enzyme-linked lectin sorbent assay (ELLSA), and lectin-probed western blotting to compare three production batches. The batches shared common mannose- and galactose-reactive signals but displayed marked heterogeneity in low-abundance fucosylated and sialylated epitopes. Haptenic sugar inhibition and targeted digestion with α(1,6)-fucosidase and α(2,3)-neuraminidase confirmed lectin specificity and showed that some lectin-reactive motifs were partially masked in native films. In light of the known restriction of classical Type I collagen glycosylation to galactosyl- and glucosylgalactosyl-hydroxylysine, together with physicochemical evidence consistent with residual non-collagenous components, the complex fucosylated and sialylated signals detected here most likely arise predominantly from co-purified collagen-associated extracellular-matrix glycoproteins rather than from the collagen α-chains alone. Functionally, all Col-I-coated surfaces strongly promoted MDA-MB-231 cell adhesion, while glycosidase treatment produced batch-dependent visual trends in wound-closure kinetics without significant differences at matched time points. Overall, this work identifies a hidden source of batch variability in commercial collagen biomaterials and positions lectin profiling as an accessible quality-control tool for characterizing the full glycoprotein composition of collagen raw materials.</p>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"114 9","pages":""},"PeriodicalIF":3.8,"publicationDate":"2026-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.a.70148","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148878308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Brazilian Red Propolis Nanoparticles: In Vitro Anticancer Effects in Female-Associated Malignancies and In Vivo Toxicity in an Alternative Model","authors":"Isabela A. Justino,&nbsp;Caroline E. A. Botteon,&nbsp;Iasmin R. S. Ferreira,&nbsp;Maria Eugênia B. Rocha,&nbsp;Andréia Marincek,&nbsp;Gláucia Rigotto Caruso,&nbsp;Jennyfer A. Aldana-Mejia,&nbsp;Marcia Regina von Zeska Kress,&nbsp;Jairo K. Bastos,&nbsp;Priscyla D. Marcato","doi":"10.1002/jbm.a.70124","DOIUrl":"https://doi.org/10.1002/jbm.a.70124","url":null,"abstract":"<p>This study explores the development and characterization of polymeric nanoparticles encapsulating Brazilian red propolis extract (BRPE) for application in ovarian (OVCAR-3) and breast (MDA-MB-231) cancers. The nanoparticles (NCBRPE), produced by nanoprecipitation, were evaluated for size, polydispersity index (PdI), zeta potential, and encapsulation efficiency (EE). Stability assessments over 365 days confirmed preserved physicochemical properties. Release kinetics, evaluated using a dialysis membrane assay and UPLC-MS, revealed that vestitol and formononetin followed the Peppas-Korsmeyer model, while biochanin A followed Higuchi kinetics, indicating diffusion-controlled mechanisms. Flow cytometry and confocal microscopy showed lower uptake of NCBRPE compared to free BRPE, though uptake was significantly higher in OVCAR-3 cells, suggesting receptor-mediated internalization. Cell cycle analysis revealed that NCBRPE, but not BRPE, induced a shift from G1 to S phase, potentially impairing cell proliferation. Migration assays demonstrated significant inhibition of OVCAR-3 cell migration by both BRPE and NCBRPE. Acute toxicity evaluation in <i>Galleria mellonella</i> confirmed the safety of NCBRPE, with over 80% survival at doses up to 50 mg/kg. These results demonstrate that nanoencapsulation enhances the stability, release control, and therapeutic activity of BRPE, supporting its potential as a multifunctional nanoplatform for treating female cancers, particularly ovarian carcinoma.</p>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"114 9","pages":""},"PeriodicalIF":3.8,"publicationDate":"2026-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.a.70124","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148878312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}