Journal of biomedical materials research. Part A最新文献

{"title":"Electrospun Silk Fibroin/Poly(Vinyl Alcohol) Nanofibrous Dressings Co-Loaded With Teicoplanin and Liposomal Curcumin: Fabrication, Physicochemical Characterization, and Antibacterial Performance","authors":"Fariba Alizadeh Eghtedar,&nbsp;Jebrail Movaffagh,&nbsp;Amir Mahdi Molavi,&nbsp;Fatemeh Gheybi,&nbsp;Zoleikha Azari,&nbsp;Seyedeh Najibeh Nasiri,&nbsp;Alireza Sadeghi-Avalshahr,&nbsp;Simin Nazarnezhad","doi":"10.1002/jbma.70040","DOIUrl":"10.1002/jbma.70040","url":null,"abstract":"<div>\u0000 \u0000 <p>Electrospun silk fibroin (SF)/poly(vinyl alcohol) (PVA) nanofibrous mats co-loaded with teicoplanin (Tp) and nanoliposomal curcumin (LC) were fabricated to combine extracellular matrix (ECM) mimetic architecture with dual antimicrobial and regenerative functionality. Tp and LC were homogeneously incorporated into SF and PVA, respectively, and electrospun under optimized voltage and flow conditions to yield defect-free fibers. Morphological analysis confirmed a consistent nanofiber diameter and a water uptake of 364.17% ± 42.25%, while in vitro degradation in PBS progressed to 45.74% ± 3.99% mass loss after 28 days. Tensile testing demonstrated a breaking strength of 5.39 MPa, indicating sufficient mechanical integrity for wound application. Drug-release assays revealed a biphasic profile for Tp—an initial burst of 666.31 ± 6.85 μg/mL within the first 24 h, followed by sustained liberation over 4 weeks—whereas curcumin exhibited a steady release rate. Cytocompatibility studies on dermal fibroblasts showed 80.88% ± 1.60% viability, and hemolysis remained below 0.13% ± 0.03%, confirming hemocompatibility. In antimicrobial evaluations, the composite dressings achieved synergistic antibactericidal activity against <i>Staphylococcus aureus</i> and <i>Pseudomonas aeruginosa</i>, outperforming single-agent controls. These findings substantiate the T@S/LC@P scaffold as a versatile, infection-resistant dressing, promising accelerated wound healing and preventing microbial colonization.</p>\u0000 </div>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"114 3","pages":""},"PeriodicalIF":3.9,"publicationDate":"2026-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147346120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research on the Combined Effects of miRNAs and Immunomodulatory Peptides on Bone Regeneration in an Inflammatory Bone Immunological Milieu","authors":"Yizhe Fu,&nbsp;Xinxiong Xia,&nbsp;Chang Liu,&nbsp;Sijing Jiang,&nbsp;Shuang Lai,&nbsp;Xun Xiao,&nbsp;Lun Yuan,&nbsp;Yandong Mu","doi":"10.1002/jbm.a.70052","DOIUrl":"10.1002/jbm.a.70052","url":null,"abstract":"<div>\u0000 \u0000 <p>The development of bone regenerative substitutes capable of orchestrating osteogenesis within inflammatory immune microenvironments remains a critical challenge. This study investigates the dual-functional immunomodulatory peptide DP7-C as a microRNA (miRNA) co-delivery system to regulate osteogenic differentiation and macrophage polarization synchronously. Through systematic screening of DP7-C/miRNA nanocomplexes (miR-21, -26a, -29a, -34a, -124, -125a) in bone marrow mesenchymal stem cells (BMSCs) and RAW264.7 macrophages, we identified DP7-C/miR-124 as the optimal nanocomplex, demonstrating synergistic osteoimmunomodulatory effects. Results demonstrated that the DP7-C/miR-124 combination raised the expression of anti-inflammatory factors in inflammatory macrophages and decreased the expression of pro-inflammatory factors. It also stimulated the production of osteogenesis-related proteins BMP2 and Runx2 to promote BMSC osteogenesis. Mechanistic studies revealed bidirectional cellular crosstalk, where DP7-C/miR-124 enhanced IL-10-mediated anti-inflammatory macrophage polarization while reciprocally promoting BMSC differentiation through paracrine modulation. These findings establish DP7-C/miRNA nanocomplexes as next-generation osteoimmunomodulatory biomaterials that concurrently resolve inflammation and amplify bone regeneration through epigenetic-immune circuit regulation, offering a promising strategy for functionalized bone defect repair in inflammatory microenvironments.</p>\u0000 </div>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"114 3","pages":""},"PeriodicalIF":3.9,"publicationDate":"2026-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147319216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Murine Biocompatibility Evaluation of an Albumin-Derived Complex and Nanoparticle Delivery System for Ocular Applications","authors":"Amna Abdalbaqi,&nbsp;Nagaraj Kerur,&nbsp;Matthew P. Ohr,&nbsp;Andre F. Palmer,&nbsp;Katelyn E. Swindle-Reilly","doi":"10.1002/jbm.a.70058","DOIUrl":"10.1002/jbm.a.70058","url":null,"abstract":"<p>Age-related macular degeneration (AMD) is a leading cause of irreversible vision loss in the aging population, with no curative treatment currently available. Current therapies primarily target late-stage symptoms and are limited by their frequent and invasive intravitreal (IVT) injections. To address oxidative stress-induced inflammation mechanisms relevant to early retinal degeneration, we developed a heme-bound human serum albumin (heme-albumin) complex designed to transiently induce heme oxygenase-1 (HO-1), a cytoprotective enzyme with antioxidant and anti-inflammatory effects. Polydopamine nanoparticles (PDA NPs) were selected as a delivery system due to their ability to scavenge reactive oxygen species (ROS) and degrade under oxidative environments. A previous in vitro study demonstrated that heme-albumin-loaded PDA NPs reduce oxidative damage and inflammatory signaling in retinal pigment epithelium (RPE) cells. This study evaluates the in vivo biocompatibility of IVT-administered heme-albumin and unloaded PDA NPs as independent components in a murine model. At the tested doses, both components showed minimal cytotoxicity with preservation of retinal structure, establishing biocompatible dosing for future evaluation in retinal disease models.</p>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"114 3","pages":""},"PeriodicalIF":3.9,"publicationDate":"2026-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.a.70058","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147313816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inclusion of Chondroitin Sulfate Into a Gelatin Hydrogel Shifts Local and Global Mechanical Behavior and Fibrochondrogenic Phenotype for Applications in Insertional Tissue Engineering","authors":"Kyle B. Timmer,&nbsp;Michael Xu,&nbsp;Brendan A. C. Harley","doi":"10.1002/jbm.a.70057","DOIUrl":"10.1002/jbm.a.70057","url":null,"abstract":"<p>Glycosaminoglycans (GAGs) like chondroitin sulfate (CS) influence both mechanical properties and biological signals within the tissue microenvironment. CS modifications have been prevalent in a range of biomaterial design strategies, particularly those with a focus on wound healing. Here, we investigate the impact of CS incorporation within a thiolated gelatin (Gel-SH) hydrogel previously established as a promising biomaterial for tendon-to-bone entheseal repair, reporting a dual biological and mechanical effect. We show that CS inclusion increases mesenchymal stem cell metabolic activity and osteo-tendinous differentiation patterns in the Gel-SH biomaterial. Additionally, we demonstrate that inclusion of CS into a Gel-SH hydrogel insertional zone used to link dissimilar tendon and bone specific collagen scaffolds induces favorable local changes in stress–strain behavior. We further show that the mode of incorporation, free incorporation of CS versus covalent tethering of oxidized CS (CSO), clearly impacts these observed effects. Overall, these results highlight promising new motifs to modulate Gel-SH hydrogels for greater promotion of enthesis-associated behavior in resident hMSCs; further, they offer broad insight into design strategies and key considerations for modification of multicompartment materials, namely in consideration of incorporation methods and on the interplay of mechanical and biological properties.</p>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"114 3","pages":""},"PeriodicalIF":3.9,"publicationDate":"2026-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13007498/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147313745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plasma-Assisted KR-12 Conjugated PLGA Nanofibers With Dual Osteogenic and Biofilm-Inhibitory Activity","authors":"Günnur Pulat,&nbsp;Eda Bilgiç,&nbsp;Buse Sezer","doi":"10.1002/jbm.a.70059","DOIUrl":"10.1002/jbm.a.70059","url":null,"abstract":"<p>Multidrug-resistant bacterial infections pose a significant challenge in bone tissue engineering, primarily due to the formation of biofilms on implant surfaces, which can impede osteointegration. KR-12, a cationic antimicrobial peptide (AMP) with dual osteoinductive and biofilm-inhibitory properties, represents a promising strategy to address this issue. Poly(lactic-co-glycolic acid) (PLGA) electrospun nanofiber (NF) scaffolds offer biocompatibility, tunable morphology, and support for cell adhesion and proliferation, making them ideal for bone regeneration. While cold atmospheric plasma (CAP) treatment has been explored to enhance peptide functionalization, covalent conjugation of KR-12 to PLGA electrospun NFs has not yet been reported. In this study, KR-12 was incorporated into electrospun PLGA NFs to create a dual-functional scaffold that promotes osteogenic differentiation while inhibiting biofilm formation. Scaffold surface properties were characterized by scanning electron microscopy (SEM) and contact angle measurements, and peptide incorporation was confirmed via fluorescein isothiocyanate (FITC) labeling and FTIR spectroscopy. Human bone marrow-derived mesenchymal stem cells cultured on KR-12-functionalized NFs exhibited enhanced alkaline phosphatase (ALP) activity, calcium and collagen deposition, and upregulated expression of collagen type I (COL1), osteopontin (OPN), and osteocalcin (OCN), as well as positive immunofluorescence staining. Antibacterial and biofilm formation inhibition activities were evaluated against multidrug-resistant MRSA and <i>P. aeruginosa</i>, as well as non-MDR <i>E. coli</i> and <i>S. aureus</i>, demonstrating potent inhibition of biofilm formation. KR-12-functionalized PLGA NFs thus provide a dual-functional platform for infection-resistant bone tissue regeneration, combining osteogenic support with potent inhibition of biofilm formation.</p>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"114 3","pages":""},"PeriodicalIF":3.9,"publicationDate":"2026-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.a.70059","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147313821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Threshold Optimization of In Situ HAPnanoclay in Polymeric Scaffolds to Enhance Biomechanical Response","authors":"Pooyan Vahidi Pashaki,&nbsp;Priyanka Kumari,&nbsp;Preetham Ravi,&nbsp;Sharad Jaswandkar,&nbsp;Benjamin Noonan,&nbsp;Kalpana S. Katti,&nbsp;Dinesh R. Katti","doi":"10.1002/jbm.a.70054","DOIUrl":"10.1002/jbm.a.70054","url":null,"abstract":"<div>\u0000 \u0000 <p>Developing bone-mimetic tissue engineering scaffolds with tunable mechanical and biological properties is vital to overcoming obstacles in bone repair and creating realistic 3D bone models. This study utilizes montmorillonite clay (MMT) modified with amino valeric acid, featuring in situ HAP mineralization within the clay galleries, henceforth referred to as in situ HAPnanoclay. Three-dimensional scaffolds were fabricated from polymer clay nanocomposites (PCNs), where varying the amount of in situ HAPnanoclay influenced both their mechanical and biological performance. SEM and EDS analyses confirmed that the in situ HAPnanoclay was uniformly dispersed within the PCL matrix. Despite being present in small amounts, the in situ HAPnanoclay significantly enhanced the scaffolds' mechanical behavior. Incorporating as little as 1% in situ HAPnanoclay established the threshold for noticeable improvements in mechanical properties compared to pure PCL scaffolds. Cell viability studies demonstrated the scaffolds' biocompatibility, showing significantly increased cell viability when the HAPnanoclay content exceeded 3%. Additionally, the scaffolds supported osteogenic differentiation of human mesenchymal stem cells (hMSCs), with ECM mineralization improving across all HAPnanoclay loadings. Moreover, scaffolds with 5% or more in situ HAPnanoclay exhibited a substantial increase in mineralization after 23 days, identifying 5% loading as a critical threshold for enhanced biomineralization. 3D PCL/in situ HAPnanoclay scaffolds demonstrated tunable mechanical and biological properties through varying clay contents. This study is the first to report the threshold percentages of in situ HAPnanoclay modified with amino valeric acid necessary to significantly improve mechanical strength and biological performance in PCN-based scaffolds for bone regeneration.</p>\u0000 </div>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"114 3","pages":""},"PeriodicalIF":3.9,"publicationDate":"2026-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147273679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D Printing Polycaprolactone-Gelatin for Musculoskeletal Tissue Engineering","authors":"Elaine Lui,&nbsp;Masanori Kobayashi,&nbsp;Charu Jain,&nbsp;Hirotsugu Maekawa,&nbsp;Jiannan Li,&nbsp;Seyedsina Moeinzadeh,&nbsp;Anthony An-Fa Dahm Chen,&nbsp;Weston Allen-Hicks,&nbsp;Benjamin Levi,&nbsp;Shuichi Matsuda,&nbsp;Toshiyuki Kawai,&nbsp;Yunzhi Peter Yang","doi":"10.1002/jbm.a.70051","DOIUrl":"10.1002/jbm.a.70051","url":null,"abstract":"<div>\u0000 \u0000 <p>In musculoskeletal tissue engineering, there is a need for bone implants that are biocompatible, resorbable, promote tissue regeneration, and degrade at a rate matching healing. Polycaprolactone (PCL), an FDA-approved biodegradable and bioinert polymer, can be functionalized with natural components without harsh crosslinking. This study presents the first demonstration of a homogeneous bulk polycaprolactone-gelatin (PCL-gelatin, PG) composite containing self-assembled gelatin nanoparticles that retain bioactivity despite thermal processing for 3D printing applications. PG composites with varying gelatin content (10%, 20%, and 30%) and β-tricalcium phosphate incorporation were fabricated through casting and melt processing into printable filaments at 110°C. Comprehensive characterization using mechanical testing, contact angle measurements, FTIR, TGA, EDS, and SEM confirmed homogeneous gelatin distribution as nanoscale particles throughout the PCL matrix, with systematic increases in hydrophilicity and enhanced mechanical properties proportional to gelatin content. Accelerated degradation studies revealed tunable degradation rates correlated with gelatin concentration, while in vitro studies with human mesenchymal stem cells demonstrated enhanced proliferation and early osteogenic differentiation markers, particularly in PG30 compositions. Subcutaneous implantation in rats over 24 weeks showed biocompatibility comparable to PCL with minimal inflammatory response and biphasic degradation behavior characterized by initial swelling followed by controlled volume reduction. In critical-size femoral defects, PG30 exhibited superior early mechanical properties and increased preosteoblast density at bone interfaces compared to PCL and PCL-TCP controls at 4 weeks. This developed fabrication methodology enables precise spatial control through 3D printing while preserving gelatin bioactivity. This approach offers a promising advancement for tissue engineering applications requiring enhanced cellular interactions and controlled degradation.</p>\u0000 </div>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"114 3","pages":""},"PeriodicalIF":3.9,"publicationDate":"2026-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146215164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In Vitro Evaluation of Escherichia coli and Staphylococcus aureus Translocation in 3D Printed Material","authors":"Ashma Sharma,&nbsp;Joshua Prince,&nbsp;A-Andrew D. Jones III","doi":"10.1002/jbm.a.70050","DOIUrl":"10.1002/jbm.a.70050","url":null,"abstract":"<p>Vascular graft infection is a rare but life-threatening condition, primarily occurring after 30 days post-surgery. Meta-analysis has shown that antimicrobial coatings on graft materials do not prevent these infections. Moreover, infections still occurs even though studies have shown that there is no bacterial proliferation or bacterial penetration of common vascular graft material. The time frame of infection, meta-analysis, and in situ studies suggest that bacteria present at the suture site are introduced into the surrounding tissue or that systemically circulating bacteria may be surviving, proliferating, diffusing slowly, and evading host immune defense in synthetic vascular grafts. <i>De novo</i> vascular graft materials, such as tissue-engineered vascular graft material and decellularized vasculature may provide an in situ platform for studying survival, proliferation, and diffusion in tissue and tissue-like materials. In this study, we used confocal microscopy to image the penetration depth of bacteria over time as a proxy for the diffusion of <i>Staphylococcus aureus</i> and <i>Escherichia coli</i> into alginate, GelMA, and decellularized porcine vascular tissue. We quantified viable bacteria breakthrough as a function of biomaterial type. We found that the penetration depth over time was similar in all three biomaterials, however <i>E. coli</i> broke through much less from tissue than from engineered materials, while <i>S. aureus</i> had higher breakthrough in the GelMa but otherwise equal rates. These results point to the possibility of interstitial growth control relative to surface coatings as a future target for engineering infection resistance in engineered vascular grafts.</p>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"114 3","pages":""},"PeriodicalIF":3.9,"publicationDate":"2026-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.a.70050","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146208474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Response to “Geara Et Al., Poly-L-Lactic Acid Microspheres Promote Skin Rejuvenation via Enhanced Fibroblast Function”","authors":"Luiz Eduardo Avelar,&nbsp;Li Ma,&nbsp;Alan Widgerow,&nbsp;Daniel Bråsäter","doi":"10.1002/jbma.70027","DOIUrl":"10.1002/jbma.70027","url":null,"abstract":"&lt;p&gt;We read with interest the article published by Geara et al., entitled “Poly-L-Lactic Acid Microspheres Promote Skin Rejuvenation via Enhanced Fibroblast Function [&lt;span&gt;1&lt;/span&gt;],” studying two commercially available esthetic poly-L-lactic acids for post-injection. The authors specifically compare PLLA-LANSYNPRO, marketed as JULÄINE, Nordberg Medical AB, Solna [&lt;span&gt;2&lt;/span&gt;], initially registered in the Republic of China as a poly-L-lactic acid (PLA; LoviSelle, Changchun Sinobiom Co. Ltd., Changchun, China [&lt;span&gt;2&lt;/span&gt;]) and PLLA-SCA; Sculptra, Galderma, Uppsala. These two esthetic injectables were compared at 24–72 h in single cell in vitro and ex vivo experiments examining selected mRNA expressions and basic ex-vivo immunohistochemistry staining.&lt;/p&gt;&lt;p&gt;As Geara et al. correctly note, several esthetic biostimulators are now marketed worldwide for esthetic use, including a variety of poly-lactic acid formulations and calcium hydroxyapatite (CaHA) dermal injectables. These differ in terms of product compositions, particle characteristics such as shape and size, molecular aspects, and have achieved various levels of evidence of clinical effect including pivotal clinical trial outcome data published in the literature, resulting in different approved esthetic indications and approved reconstitution processes, as reflected in the respective manufacturer's instructions for use (IFU) [&lt;span&gt;3, 4&lt;/span&gt;]. Per JULÄINE's IFUs and described in Geara et al., PLLA-LANSYNPRO for esthetic use has a product composition of 150 mg of poly-L-lactic acid, 45 mg of sodium carboxymethylcellulose (CMC) and 145 mg of non-pyrogenic mannitol with a reconstitution volume of 5 mL Sterile water for injection (SWFI) or 0.9% saline with the intended use for nasolabial folds (NLF). PLLA-LANSYNPRO (JULÄINE) currently has 2-month, open label, non-randomized, interim data published [&lt;span&gt;5&lt;/span&gt;] supporting clinical outcomes in human subjects. PLLA-SCA (Sculptra) is on the other hand an FDA approved regenerative biostimulator with a proven effectiveness, safety and a treatment duration beyond 2 years [&lt;span&gt;6-9&lt;/span&gt;] based on three (3) pivotal trials. PLLA-SCA has a product composition of 150 mg of poly-L-lactic acid, 90 mg of CMC and 127.5 mg of mannitol, with an immediate 8 mL SWFI reconstitution volume with the additional option to add 1 mL 2% lidocaine to the final injection volume (9 mL) per IFU [&lt;span&gt;4&lt;/span&gt;].&lt;/p&gt;&lt;p&gt;Of concern is the in vitro/ex vivo experimental early timepoint-dependent study design including selected reconstitution volumes impacting data interpretation and study conclusions of the published work. First, a major limitation is that the methodology for preparing the PLLA product does not adhere to the approved PLLA-SCA IFU [&lt;span&gt;4&lt;/span&gt;] or the literature, and the methodology is insufficiently descriptive, given that the authors describe the study as clinically relevant. The preparation of experimental in vitro solutions and ex vivo injection","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"114 2","pages":""},"PeriodicalIF":3.9,"publicationDate":"2026-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbma.70027","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146198324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bilayer Fiber-Reinforced Composite-Hydrogel Scaffolds With Bioactive Glass for Bone Tissue Regeneration","authors":"Mona Gibreel,&nbsp;Roope Ohlsbom,&nbsp;Leila Perea-Lowery,&nbsp;Lippo Lassila,&nbsp;Paula Puistola,&nbsp;Karoliina Hopia,&nbsp;Susanna Miettinen,&nbsp;Anni Mörö,&nbsp;Pekka K. Vallittu","doi":"10.1002/jbma.70037","DOIUrl":"10.1002/jbma.70037","url":null,"abstract":"<p>Bone tissue regeneration for large defects presents a significant challenge, demanding scaffolds that combine robust mechanical support alongside a bioactive environment. Hydrogels represent a promising solution for bone regeneration due to their biocompatibility, tunable properties, and crosslinked three-dimensional (3D) networks mimicking the natural extracellular matrix (ECM). However, their mechanical properties remain suboptimal for restoring bone defects effectively. This study introduces a novel bilayer laminate scaffold, integrating a biostable fiber-reinforced composite (FRC) with a biodegradable, 3D-printed hyaluronic acid (HA)-based hydrogel. To enhance bioactivity, bioactive glass (BAG) was incorporated into the hydrogel layer. Comprehensive characterization confirmed the scaffold's chemical and morphological properties, as well as its controlled degradation, sustained ion release, and bioactivity. Additionally, the study revealed that the BAG-induced alkaline pH shift (up to 9.24) affected hydrazone crosslinking efficiency, resulting in reduced hydrogel stiffness (86 ± 8 Pa versus 150 ± 4 Pa in control). The system showed excellent cytocompatibility, supporting high viability and proliferation of human bone marrow stem cells (BMSCs) embedded within the hydrogel component. The developed scaffolds promoted osteogenic differentiation, as evidenced by increased ALP activity and upregulated expression of osteogenic marker genes. Nevertheless, BAG incorporation did not enhance early osteogenic differentiation compared to control scaffolds. In conclusion, this bilayer scaffold offers a promising platform for bone tissue engineering (TE), providing some insights into the chemical interplay between inorganic fillers and hydrogel matrix for optimizing future scaffold designs.</p>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"114 2","pages":""},"PeriodicalIF":3.9,"publicationDate":"2026-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbma.70037","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146159699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}