Journal of biomedical materials research. Part A最新文献

{"title":"Mesenchymal Stromal Cell Chondrogenic Differentiation Induced by Continuous Stiffness Gradient in Photocrosslinkable Hydrogels","authors":"Sabrina C. Mierswa,&nbsp;Erika E. Wheeler,&nbsp;Ayla N. Apsey,&nbsp;Oju Jeon,&nbsp;Eben Alsberg,&nbsp;J. Kent Leach","doi":"10.1002/jbm.a.37928","DOIUrl":"https://doi.org/10.1002/jbm.a.37928","url":null,"abstract":"<div>\u0000 \u0000 <p>Chondrogenic differentiation of stem and progenitor cells is dependent on the biophysical properties of the surrounding matrix. Current biomaterials-based approaches for chondrogenesis are limited to discrete platforms, slowing our ability to interrogate the role of mechanical cues such as substrate stiffness and other signals. Thus, novel platforms must incorporate a range of biophysical properties within a single construct to effectively assess changes in cell response. We encapsulated human mesenchymal stromal cells (MSCs) within biodegradable, photocurable oxidized, and methacrylated alginate (OMA). Cell-laden hydrogels were crosslinked when exposed to light through a grayscale photomask to form substrates with a continuous stiffness gradient. We also tested the influence of the adhesive ligand Arg-Gly-Asp (RGD) on chondrogenic differentiation. Compared to unmodified gels possessing uniform biophysical properties, RGD-modified OMA hydrogels with the same modulus promoted chondrogenic differentiation of MSCs as evidenced by gene expression, matrix deposition, and histological analysis. MSCs entrapped in OMA hydrogels exhibiting a biologically relevant stiffness gradient (2–13 kPa over 8 mm) demonstrated increased chondrogenic differentiation with increases in stiffness. MSC chondrogenic differentiation was dependent upon the ability to mechanosense the modulus of the surrounding matrix, confirmed by the addition of Latrunculin A (LatA), a soluble inhibitor of actin polymerization. These findings validate a methodology for customizing hydrogel platforms for chondrogenic differentiation and identifying the interplay of key variables to instruct cell function.</p>\u0000 </div>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 5","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143939017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Versatile Coating via Programmed Assembly of miR-155 Inhibitor and Endothelial Affinity Peptide","authors":"Linsen Li,&nbsp;Di An,&nbsp;Jinhu Zhao,&nbsp;Yue Yu,&nbsp;Yating Zeng,&nbsp;Xiayan Yang,&nbsp;Qifeng Yu,&nbsp;Ke Kang,&nbsp;Yao Wu,&nbsp;Qiangying Yi","doi":"10.1002/jbm.a.37923","DOIUrl":"https://doi.org/10.1002/jbm.a.37923","url":null,"abstract":"<div>\u0000 \u0000 <p>Medical implants serve as pivotal therapeutic interventions for cardiovascular diseases. Nevertheless, challenges including endothelial injury, excessive smooth muscle cell migration, and inflammatory responses associated with implantation hinder their long-term adoption in vivo. In this work, a versatile coating of cardiovascular implants was constructed by the remarkable adhesive properties of dopamine and the precision and efficiency of click chemistry. The functional coating was obtained through programmed assembly of heparin, miR-155 inhibitor, and endothelial cell affinity peptide. Cellular experiments have demonstrated that this coating could modulate macrophage phenotype through anti-miR155 inhibitors, thereby inhibiting the release of inflammatory factors and improving the inflammatory environment on the surface. Furthermore, this coating could also promote the adhesion and proliferation of endothelial cells while inhibiting the excessive proliferation of smooth muscle cells, thereby ensuring the endothelialization of the implant surface. Additionally, animal experiments have revealed the coating's exceptional anticoagulant properties, significantly reducing the formation of thrombus. With its simple and efficient preparation process and excellent performance, this coating emerges as a promising candidate for promoting early endothelialization and improving the performance of cardiovascular implants.</p>\u0000 </div>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 5","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143939113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PDGF-Releasing Hydrogels for Enhanced Proliferation of Human Nucleus Pulposus Cells","authors":"Takanori Fukunaga,&nbsp;Joseph J. Pearson,&nbsp;Ryan Cree Miller,&nbsp;Changli Zhang,&nbsp;Mohammed Lakrat,&nbsp;Lisbet Haglund,&nbsp;Martha Elena Diaz-Hernandez,&nbsp;Johnna S. Temenoff,&nbsp;Hicham Drissi","doi":"10.1002/jbm.a.37918","DOIUrl":"https://doi.org/10.1002/jbm.a.37918","url":null,"abstract":"<div>\u0000 \u0000 <p>Hydrogels offer a promising solution for sustained and controlled drug delivery and cell-tissue biocompatibility. In the intervertebral disc (IVD), delivering growth factors faces challenges due to the antagonistic inflammatory environment and continuous mechanical stress, which can degrade biological agents and may reduce their local activity. To address this, we investigated the prolonged release of platelet-derived growth factor isoforms BB (PDGF-BB) and AB (PDGF-AB) by using N-desulfated heparin methacrylamide (Hep^-N) crosslinked within matrix-metalloproteinase sensitive poly(ethylene glycol) (PEG) hydrogels. Using electrostatic interactions between the heparin derivative and PDGF, we optimized a sustained release dose of PDGF-BB from the hydrogel in the presence of collagenase to mimic the in vivo environment. We then assessed the effects of PDGF released from PEG-hydrogel on human nucleus pulposus (NP) Cells. The MTT assay confirmed that 100 and 200 ng doses significantly increased cell viability by 2.52-fold and 2.46-fold on Day 3, respectively. RT-qPCR analysis revealed that PDGF-AB and PDGF-BB upregulated the expression of proliferation marker Ki-67 (MKI67) on both Day 3 and Day 5. Additionally, collagen type II alpha 1 chain (COL2A1) was significantly upregulated in the PDGF-AB group on Day 5, indicating potential anabolic effects. These findings could pave the way for long-term in vivo studies on sustainable PDGF treatment for IVD degeneration.</p>\u0000 </div>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 5","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143925950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation, Physicochemical Characterization, and In Vitro and In Vivo Osteogenic Evaluation of Reinforced PLLA-PLCL/HA Resorbable Membranes","authors":"Zheng Fu,&nbsp;Jing Wang,&nbsp;Yuhan Wu,&nbsp;Wenyi Zeng,&nbsp;Chenguang Zhang,&nbsp;Yang Sun,&nbsp;Xiaoshan Fan,&nbsp;Yucheng Huang,&nbsp;Feilong Deng,&nbsp;Jiayun Xu","doi":"10.1002/jbm.a.37925","DOIUrl":"https://doi.org/10.1002/jbm.a.37925","url":null,"abstract":"<div>\u0000 \u0000 <p>This study aimed to develop reinforced poly(L-lactide)-poly(L-lactide-co-ε-caprolactone)/hydroxyapatite (PLLA-PLCL/HA) resorbable membranes for guided bone regeneration (GBR), focusing on optimizing the degradation rate by adjusting PLLA molecular weight. We aimed to achieve a balance between mechanical strength and bioactivity to enhance the efficacy of bone regeneration. PLLA-PLCL/HA membranes with varying degradation rates were fabricated by modifying the molecular weight of PLLA. The membranes were characterized using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), andx-ray diffraction (XRD). Mechanical properties were evaluated using three-point bending tests, and in vitro cytocompatibility was assessed through MC3T3-E1 cell adhesion and proliferation. For in vivo experiments, a cranial defect mouse model was used to investigate degradation and osteogenic potential, and bone regeneration was evaluated using micro-CT, histological staining, and immunohistochemistry. The reinforced membranes exhibited superior bending strength compared to collagen membranes. The in vitro studies confirmed excellent cytocompatibility, and in vivo results showed that membranes with slower early stage degradation promoted bone regeneration, emphasizing the importance of degradation control in GBR membranes. The optimized PLLA-PLCL/HA membranes, which combine enhanced mechanical properties and controlled biodegradability, are promising candidates for clinical GBR applications.</p>\u0000 </div>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 5","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143919533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vascular Endothelial Growth Factor-Mimetic Peptide and Mitochondria-Targeted Antioxidant-Loaded Hydrogel System Improves Repair of Myocardial Infarction in Mice","authors":"Qingling Xu,&nbsp;Xinhui Chen,&nbsp;Shuwei Sun,&nbsp;Chunyige Zhao,&nbsp;Linxin Shi,&nbsp;Han Cheng,&nbsp;Ying Liu,&nbsp;Chunying Shi,&nbsp;Xiang Ao","doi":"10.1002/jbm.a.37924","DOIUrl":"https://doi.org/10.1002/jbm.a.37924","url":null,"abstract":"<div>\u0000 \u0000 <p>Myocardial infarction (MI) is a pathological state characterized by persistent ischemia of the heart. Following MI, the structural and functional remodeling of the myocardium and vasculature involves oxidative stress and mitochondrial dysfunction, which exacerbate myocardial injury. Currently, there are limited effective treatments available to alleviate MI-induced damage. Vascular endothelial growth factor-mimetic (QK) peptides and mitochondria-targeted Szeto–Schiller (SS31) peptides have been extensively investigated for their therapeutic potential in various ischemic cardiomyopathies. However, traditional topical agents used in myocardial ischemia treatment suffer from limitations such as transient retention or undesirable diffusion of the drug. Consequently, a controlled drug delivery system capable of delivering QK and SS31 has gained significant attention for repair. In this study, we constructed self-assembled nanofibrous hydrogels incorporating QK and SS31 with customizable peptide amphiphilic (PA) molecules, resulting in PA1-QK and PA2-SS31 formulations. In vitro experiments demonstrated that both QK and SS31 effectively inhibited mitochondrial damage and apoptosis in a cellular hypoxia/reoxygenation (H/R) model. In vivo studies using a mouse MI model revealed that PA1-QK and PA2-SS31 significantly promoted vascular regeneration, attenuated mitochondrial dysfunction and apoptosis, and facilitated the recovery of cardiac structure and function. These results suggest that PA1-QK and PA2-SS31-loaded self-assembled nanofiber hydrogels represent an effective drug delivery system for promoting regenerative repair of myocardium and blood vessels following MI.</p>\u0000 </div>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 5","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143919525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Rapid Manual Solid Phase Peptide Synthesis Method for High-Throughput Peptide Production","authors":"Clyde Overby,&nbsp;Brittany Abraham,&nbsp;Emmanuella Adjei-Sowah,&nbsp;Alyson March,&nbsp;Kevin Ling,&nbsp;Sayantani Basu,&nbsp;Danielle S. W. Benoit","doi":"10.1002/jbm.a.37922","DOIUrl":"https://doi.org/10.1002/jbm.a.37922","url":null,"abstract":"<p>Solid phase peptide synthesis (SPPS) techniques are critical for developing and using peptides in various biomedical applications. However, typical synthesis routes used in SPPS are either resource-intensive (e.g., with automated synthesis or commercial services) or time-consuming (e.g., with manual benchtop synthesis). Here, a rapid manual synthesis method was developed to produce up to 8 peptides with fast cycle times simultaneously. Peptides synthesized manually were of equivalent or superior quality to those produced by in-house microwave-assisted automated peptide synthesis, with higher average crude purity of 70% compared to 50%. The method significantly reduced synthesis time, enabling the parallel coupling of up to 8 amino acids simultaneously in 15–20 min, as opposed to traditional benchtop peptide synthesis, which requires 80–150 min per amino acid. This approach offers an intermediate throughput between milligram-scale libraries and gram-scale single peptide synthesis, enabling rapid iteration for novel peptide designs without the need for expensive automated systems. As a result, peptide modifications, including incorporation of unnatural amino acids, can be explored, accelerating the development of peptides for a wide range of applications.</p>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 5","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.a.37922","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143897103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibiotic-Loaded Polymer-Calcium Phosphate Scaffold for Treating Orthopedic Device-Related Infection in a Rabbit Segmental Bone Defect Model","authors":"T. Buchholz,&nbsp;C. Siverino,&nbsp;T. F. Moriarty,&nbsp;E. J. Sheehy,&nbsp;F. J. O'Brien,&nbsp;D. Nehrbass,&nbsp;S. Arveladze,&nbsp;C. Constant,&nbsp;S. Hassouna Elsayed,&nbsp;M. Yan,&nbsp;H. A. Awad,&nbsp;S. Zeiter,&nbsp;M. J. Allen","doi":"10.1002/jbm.a.37917","DOIUrl":"https://doi.org/10.1002/jbm.a.37917","url":null,"abstract":"<div>\u0000 \u0000 <p>Treatment of orthopedic device-related infection (ORDI) generally requires a combination of medical and surgical interventions for successful treatment outcomes. Many cases are treated with a two-stage revision, whereby contaminated implants and necrotic tissues are removed, and dead space is managed with a temporary, non-resorbable polymethyl methacrylate (PMMA) spacer loaded with antibiotics. Weeks later, this is replaced with a bone graft or similar material to aid bone healing. However, this two-stage approach is quite a burden for the patient, and infection may still recur. The use of a 3D-printed, absorbable, antibiotic-releasing material that also promotes bone healing would be a promising alternative that produces the exact geometry of the missing bone and eliminates the need for a second surgery. In this study, we investigated whether a novel 3D-printed, antibiotic-loaded, osteoconductive calcium phosphate scaffold (CPS) is effective in the single-stage revision of an infected segmental bone defect model in rabbits. A 5-mm segmental defect of the radius of female New Zealand White rabbits (<i>n</i> = 64), stabilized with cerclage wire, was inoculated with <i>Staphylococcus aureus</i>. After 4 weeks, the infected bone fragment was removed, the site debrided, and the bone defect was either left empty (Control group) or filled with a PMMA spacer with gentamicin, CPS loaded with rifampicin or non-loaded CPS. The animals were also managed with systemic cefazolin for 4 weeks. An additional group received vancomycin-loaded CPS without adjunctive systemic antibiotic therapy. All animals were euthanized 8 weeks after revision and assessed by quantitative bacteriology or semiquantitative histopathology. The antibiotic-loaded scaffolds (PMMA-Genta and CPS-Rif) in the animals receiving systemic antibiotic treatment resulted in a reduction in bacterial count at euthanasia compared to controls (rabbits receiving systemic antibiotic alone and in which the defect was left empty). The PMMA-Genta induced a significant CFU reduction (<i>p</i> = 0.0486) compared to controls. The infection rate was also reduced from 80% in the control group to 50% for the groups receiving local and systemic antibiotics. The CPS-Vanco group for local delivery without adjunctive systemic antibiotic therapy resulted in a lower infection rate, but the CFUs in these samples at euthanasia were comparable with those of the control group. The findings show that treating an ODRI with PMMA-Genta yields the best results for infection eradication; however, it does not provide the reconstruction opportunity that the antibiotic-loaded CPS does. Even though it is not comparable to the PMMA-Genta, the antibiotic-loaded CPS showed a reduction in infection rates. The use of local antibiotics alone is insufficient to eradicate the infection.</p>\u0000 </div>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 5","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143883820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrospun Decellularized Skeletal Muscle Tissue/Polycaprolactone/Polyaniline as a Potential Scaffold for Muscle Tissue Engineering","authors":"Faraz Sigaroodi,&nbsp;Marziyeh Jalali Monfared,&nbsp;Masoumeh Foroutan Koudehi,&nbsp;Ramin Zibaseresht","doi":"10.1002/jbm.a.37920","DOIUrl":"https://doi.org/10.1002/jbm.a.37920","url":null,"abstract":"<div>\u0000 \u0000 <p>Skeletal muscle tissue is capable of self-healing on a small scale. However, during extensive trauma or surgery, regenerative capacities are lost due to the loss of muscle cells and extracellular matrix. Therefore, the development of tissue engineering strategies for the regeneration of muscle tissue should be considered. In this study, we electrospun decellularized skeletal muscle tissue (DSM)/polycaprolactone (PCL)/polyaniline (PANi) as a bioactive polymer composite and investigated the structural characteristics, physicochemical properties, and effect of PANi on these properties. Next, the biological and myogenic effects of scaffolds on human Wharton's jelly mesenchymal stem cells (hWJ-MSCs) were investigated. The results showed that DSM/PCL/PANi is a conductive fibrous scaffold with favorable physical and chemical properties for muscle tissue engineering; it is biocompatible with hWJ-MSCs and stimulates their morphology. Additionally, hWJ-MSCs cultured on DSM/PCL/PANi showed a significant increase in the expression of MyoD, Myogenin, and MHC. Laboratory experiments showed that the electrospun scaffold of DSM/PCL/PANi is biocompatible with favorable physical properties for the growth of stem cells and the expression of myogenic markers, which can be useful in the development of biological scaffold approaches for muscle tissue engineering.</p>\u0000 </div>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 5","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143880151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RETRACTION: PEG2000-DPSE-Coated Quercetin Nanoparticles Remarkably Enhanced Anticancer Effects through Induced Programed Cell Death on C6 Glioma Cells","authors":"","doi":"10.1002/jbm.a.37894","DOIUrl":"https://doi.org/10.1002/jbm.a.37894","url":null,"abstract":"<p>\u0000 <b>RETRACTION:</b> <span>G. Wang</span>, <span>JJ. Wang</span>, <span>J. Luo</span>, <span>L. Wang</span>, <span>XL. Chen</span>, <span>LP. Zhang</span>, and <span>SQ. Jiang</span>, “ <span>PEG2000-DPSE-Coated Quercetin Nanoparticles Remarkably Enhanced Anticancer Effects through Induced Programed Cell Death on C6 Glioma Cells</span>,” <i>Journal of Biomedical Materials Research Part A</i> <span>101</span>, no. <span>11</span> (<span>2013</span>): <span>3076</span>–<span>3085</span>, https://doi.org/10.1002/jbm.a.34607.\u0000 </p><p>The above article, published online on 25 March 2013, in Wiley Online Library (http://onlinelibrary.wiley.com/), has been retracted by agreement between the journal Editor-in-Chief, J. Kent Leach; and Wiley Periodicals LLC. A third party reported several concerns to the publisher about duplicated images in this article, including duplication of a plot between Figures 4B and 4D; duplication of an image within Figure 4E; and duplication of all images in Figure 4E with another article published by many of the same authors (Wang, et al. 2013 [https://doi.org/10.1038/cddis.2013.242]). Further investigation by the publisher uncovered evidence of duplication, manipulation, and resizing of western blot images between Figures 5D and 5E; duplication of beta-actin bands between Figures 5C, 5D, and 6E; and insertion of a band in Figure 6E. The authors responded to an inquiry by the publisher and stated that the image duplications were the result of errors during figure preparation and labeling the original data. However, the parties agree that the explanations and data supplied by the authors did not properly address the concerns raised. The retraction has been agreed to because the evidence of image duplication and manipulation fundamentally compromises the reliability of the data and conclusions reported in this article. The authors did not respond to our notice regarding the retraction.</p>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 5","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.a.37894","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143875645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Importance of Coating Surface and Composition for Attachment and Survival of Neuronal Cells Under Mechanical Stimulation","authors":"Pascal A. M. M. Vroemen,&nbsp;Adrián Seijas-Gamardo,&nbsp;Roy Palmen,&nbsp;Paul A. Wieringa,&nbsp;Carroll A. B. Webers,&nbsp;Lorenzo Moroni,&nbsp;Theo G. M. F. Gorgels","doi":"10.1002/jbm.a.37919","DOIUrl":"https://doi.org/10.1002/jbm.a.37919","url":null,"abstract":"<p>Cell culture of neuronal cells places high demands on the surface for these cells to adhere to and grow on. Native extracellular matrix (ECM) proteins are often applied to the cell culture surface. The substrate is even more important when mechanical strain is applied to the cells in culture. These cells will easily detach and die, precluding the study of how mechanical factors affect these cells. Mechanical factors are, for example, important in the eye disorder glaucoma, which is characterized by the loss of the retinal ganglion cells (RGCs), the retinal neurons that transfer the visual information from the retina via the optic nerve to the brain. High intraocular pressure is the main risk factor of glaucoma. Here, we aimed to find an optimal coating formulation for mechanical testing of the two cell types that are often used for in vitro studies on glaucoma: primary rat retinal ganglion cells (RGCs) and the neuronal PC-12 cell line. Glass and polymer coverslips as well as well plate wells were coated with various substrates: fibronectin, collagen 1, RGD peptide, polyethyleneimine (PEI), poly-D-lysine (PDL), and laminin. We used a thermomixer for 1 min at 500RPM and 37°C to apply mechanical strain and test cell attachment in medium throughput during mechanical stimulation. Cell density, morphology, and cell death were measured to evaluate the coatings. First, a screen of various surfaces and coatings was performed using PC-12 cells, after which a selection of coating strategies was tested with RGCs. For PC-12 cells, the best results were obtained using a coating with a mixture of 10 μg/mL PDL with 2 or 50 μg/mL laminin in PBS (M2). This resulted in the highest cell density, with and without mechanical stimulation. Many other coating strategies failed to provide an effective substrate for adherence and growth of PC-12 cells. Coating composition as well as coating strategy influenced cell attachment and survival. Contrary to PC-12 cells, RGCs performed better in a sequential coating of first 10 μg/mL PDL and then 2 μg/mL laminin (S2). With this protocol, RGCs showed best neurite growth and highest cell density. Based on this difference between PC-12 cells and RGCs, we conclude that the optimal coating depends on the cell type. When reporting cell culture studies, it is important to fully specify culture surface, surface treatment, and coating protocol since all these factors influence cell attachment, growth, and survival.</p>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 5","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.a.37919","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143875646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}