Journal of biomedical materials research. Part A最新文献

{"title":"Regenerated nanofibrous cellulose electrospun from ionic liquid: Tuning properties toward tissue engineering","authors":"Ingrida Pauliukaitytė,&nbsp;Darius Čiužas,&nbsp;Edvinas Krugly,&nbsp;Odeta Baniukaitienė,&nbsp;Mindaugas Bulota,&nbsp;Vilma Petrikaitė,&nbsp;Dainius Martuzevičius","doi":"10.1002/jbm.a.37798","DOIUrl":"10.1002/jbm.a.37798","url":null,"abstract":"<p>Regenerated fibrous cellulose possesses a unique set of properties, including biocompatibility, biodegradability, and high surface area potential, but its applications in the biomedical sector have not been sufficiently explored. In this study, nanofibrous cellulose matrices were fabricated via a wet-electrospinning process using a binary system of the solvent ionic liquid (IL) 1-butyl-3-methylimidazolium acetate (BMIMAc) and co-solvent dimethyl sulfoxide (DMSO). The morphology of the matrices was controlled by varying the ratio of BMIMAc versus DMSO in the solvent system. The most effective ratio of 1:1 produced smooth fibers with diameters ranging from 200 to 400 nm. The nanofibrous cellulose matrix showed no cytotoxicity when tested on mouse fibroblast L929 cells whose viability remained above 95%. Human triple-negative breast cancer MDA-MB-231 cells also exhibited high viability even after 7 days of seeding and were able to penetrate deeper layers of the matrix, indicating high biocompatibility. These properties of nanofibrous cellulose demonstrate its potential for tissue engineering and cell culture applications.</p>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142256954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High hydrostatic pressure treatment for advanced tissue grafts in reconstructive head and neck surgery","authors":"Friederike Kalle,&nbsp;Valentin Paul Stadler,&nbsp;Julia Kristin Brach,&nbsp;Vivica Freiin Grote,&nbsp;Christopher Pohl,&nbsp;Karoline Schulz,&nbsp;Michael Seidenstuecker,&nbsp;Anika Jonitz-Heincke,&nbsp;Rainer Bader,&nbsp;Robert Mlynski,&nbsp;Daniel Strüder","doi":"10.1002/jbm.a.37791","DOIUrl":"10.1002/jbm.a.37791","url":null,"abstract":"<p>The increasing importance of regenerative medicine has resulted in a growing need for advanced tissue replacement materials in head and neck surgery. Allo- and xenogenic graft processing is often time-consuming and can deteriorate the extracellular matrix (ECM). High hydrostatic pressure (HHP)-treatment could allow specific devitalization while retaining the essential properties of the ECM. Porcine connective tissue and cartilage were HHP-treated at 100–400 MPa for 10 min. Structural modifications following HHP-exposure were examined using electron microscopy, while devitalization was assessed through metabolism and cell death analyses. Furthermore, ECM alterations and decellularization were evaluated by histology, biomechanical testing, and DNA content analysis. Additionally, the inflammatory potential of HHP-treated tissue was evaluated in vivo using a dorsal skinfold chamber in a mouse model. The devitalization effects of HHP were dose-dependent, with a threshold identified at 200 MPa for fibroblasts and chondrocytes. At this pressure level, HHP induced structural alterations in cells, with a shift toward late-stage apoptosis. HHP-treatment preserved ECM structure and biomechanical properties, but did not remove cell debris from the tissue. This study observed a pressure-dependent increase of markers suggesting the occurrence of immunogenic cell death. In vivo investigations revealed an absence of inflammatory responses to HHP-treated tissue, indicating a favorable biological response to HHP. In conclusion, application of HHP devitalizes fibroblasts and chondrocytes at 200 MPa while retaining the essential properties of the ECM. Prospectively, HHP may simplify the preparation of allo- and xenogenic tissue replacement materials and increase the availability of grafts in head and neck surgery.</p>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.a.37791","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142256956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrospun poly(ε-caprolactone)/poly(glycerol sebacate) aligned fibers fabricated with benign solvents for tendon tissue engineering","authors":"Francesco Iorio,&nbsp;Mohammad El Khatib,&nbsp;Natalie Wöltinger,&nbsp;Maura Turriani,&nbsp;Oriana Di Giacinto,&nbsp;Annunziata Mauro,&nbsp;Valentina Russo,&nbsp;Barbara Barboni,&nbsp;Aldo R. Boccaccini","doi":"10.1002/jbm.a.37794","DOIUrl":"10.1002/jbm.a.37794","url":null,"abstract":"<p>The electrospinning technique is a commonly employed approach to fabricate fibers intended for various tissue engineering applications. The aim of this study is to develop a novel strategy for tendon repair through the use of aligned poly(ε-caprolactone) (PCL) and poly(glycerol sebacate) (PGS) fibers fabricated in benign solvents, and further explore the potential application of PGS in tendon tissue engineering (TTE). The fibers were characterized for their morphological and physicochemical properties; amniotic epithelial stem cells (AECs) were used to assess the fibers teno-inductive and immunomodulatory potential due to their ability to teno-differentiate undergoing first a stepwise epithelial to mesenchymal transition, and due to their documented therapeutic role in tendon regeneration. The addition of PGS to PCL improved the spinnability of the polymer solution, as well as the uniformity and directionality of the so-obtained fibers. The mechanical properties were in the range of most TTE applications, specifically in the case of PCL/PGS 4:1 and 2:1 ratios. Compared to PCL alone, the same ratios also allowed a better AECs infiltration and growth over 7 days of culture, and triggered the activation of tendon-related genes (SCX, COL1, TNMD) and the expression of tenomodulin (TNMD) at the protein level. Concerning the immunomodulatory properties, both PCL and PCL/PGS fibers negatively affected the immunomodulatory profile of AECs, up-regulating both anti-inflammatory (IL-10) and pro-inflammatory (IL-12) cytokines over 7 days of culture. Overall, PCL/PGS 2:1 fibers fabricated with benign solvents proved to be the most suitable composition for TTE application based on their topographical cues, mechanical properties, biocompatibility, and teno-inductive properties.</p>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.a.37794","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142256958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential effects of macrophage subtype-specific cytokines on fibroblast proliferation and endothelial cell function in co-culture system","authors":"Ilaha Isali,&nbsp;Phillip McClellan,&nbsp;Thomas R. Wong,&nbsp;Sara Hijaz,&nbsp;David R. Fletcher,&nbsp;Guiming Liu,&nbsp;Tracey L. Bonfield,&nbsp;James M. Anderson,&nbsp;Adonis Hijaz,&nbsp;Ozan Akkus","doi":"10.1002/jbm.a.37799","DOIUrl":"10.1002/jbm.a.37799","url":null,"abstract":"<p>Macrophages are involved in several critical activities associated with tissue repair and regeneration. Current approaches in regenerative medicine are focusing on leveraging the innate immune response to accelerate tissue regeneration and improve long-term healing outcomes. Of particular interest in this regard are the currently known, four main M2 macrophage subtypes: M2^{interleukin (IL)-4,IL-13}, M2^IC, M2^IL-10, M2^{non-selective adenosine receptor agonists (NECA)} (M2^IL-4,IL-13 → M2^NECA). In this study, rat bone marrow-derived macrophages (M₀) were polarized to each of the four subtypes M2^IL-4,IL-13 → M2^NECA and cultured for 72 h in vitro. Luminex assay results highlighted increased production of tissue inhibitor of metalloproteinases-1 (TIMP-1) for M2^IL-4,IL-13, higher amounts of transforming growth factor-beta 1 (TGF-β1) for M2^IL-10, and elevated vascular endothelial growth factor A (VEGF-A) from M2^NECA. Co-culture experiments performed with M2^IL-10 macrophages and L929 fibroblasts highlighted the increased production of soluble collagen within the media as well as higher amounts of collagen in the extracellular matrix. Human umbilical vein endothelial cells (HUVECs) were co-cultured with M2^NECA macrophages, which demonstrated an increase in intercellular adhesion molecule (ICAM) and platelet endothelial cell adhesion molecule (PECAM), as well as increased formation of endothelial tubes. The findings of this study emphasize a critical demand for further characterization and analyses of distinct M₂ subtypes and careful selection of specific macrophage populations for regeneration of specific tissue types. The current, broad classification of “M₂” may be sufficient in many general tissue engineering applications, but, as conditions are constantly in flux within the microenvironment in vivo, a higher degree of specificity and control over the initial M₂ subtype could result in more consistent long-term outcomes where macrophages are utilized as part of an overall regenerative strategy.</p>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.a.37799","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142256957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrical impedance characterization and modelling of Ti-Β implants","authors":"Paula Navarro,&nbsp;Miguel Barrera,&nbsp;Alberto Olmo,&nbsp;Yadir Torres","doi":"10.1002/jbm.a.37797","DOIUrl":"10.1002/jbm.a.37797","url":null,"abstract":"<p>Commercially pure titanium (c.p. Ti) and Ti6Al4V alloys are the most widely used metallic biomaterials in the biomedical sector. However, their high rigidity and the controversial toxicity of their alloying elements often compromise their clinical success. The use of porous β-Titanium alloys is proposed as a solution to these issues. In this regard, it is necessary to implement economic, repetitive, and non-destructive measurement techniques that allow for the semi-quantitative evaluation of the chemical nature of the implant, its microstructural characteristics, and/or surface changes. This study proposes the use of simple measurement protocols based on electrical impedance measurements, correlating them with the porosity inherent to processing conditions (pressure and temperature), as well as the chemical composition of the implant. Results revealed a clear direct relationship between porosity and electrical impedance. The percentage and/or size of the porosity decrease with an increase in compaction pressure and temperature. Moreover, there is a notable influence of the frequency used in the measurements obtained. Additionally, the sensitivity of this measurement technique has enabled the evaluation of differences in chemical composition and the detection of intermetallics in the implants. For the first time in the literature, this research establishes relationships between stiffness and electrical impedance, using approximations and models for the observed trends. All the results obtained corroborate the appropriateness of the technique to achieve the real-time characterization of Titanium implants, in an efficient and non-invasive way.</p>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2024-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.a.37797","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142256999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of multilayered artificial urethra graft for urethroplasty","authors":"Povilas Barasa,&nbsp;Egidijus Simoliunas,&nbsp;Aivaras Grybas,&nbsp;Ramune Zilinskaite-Tamasauske,&nbsp;Darius Dasevicius,&nbsp;Milda Alksne,&nbsp;Ieva Rinkunaite,&nbsp;Andrius Buivydas,&nbsp;Emilija Baltrukonyte,&nbsp;Rimgaile Tamulyte,&nbsp;Ashwinipriyadarshini Megur,&nbsp;Gilvydas Verkauskas,&nbsp;Daiva Baltriukiene,&nbsp;Virginija Bukelskiene","doi":"10.1002/jbm.a.37796","DOIUrl":"10.1002/jbm.a.37796","url":null,"abstract":"<p>To enhance the treatment of patients' urethral defects, such as strictures and hypospadias, we investigated the potential of using artificial urethral tissue. Our study aimed to generate this tissue and assess its effectiveness in a rabbit model. Two types of bioprinted grafts, based on methacrylated gelatin-silk fibroin (GelMA-SF) hydrogels, were produced: acellular, as well as loaded with autologous rabbit stem cells. Rabbit adipose stem cells (RASC) were differentiated toward smooth muscle in the GelMA-SF hydrogel, while rabbit buccal mucosa stem cells (RBMC), differentiated toward the epithelium, were seeded on its surface, forming two layers of the cell-laden tissue. The constructs were then reinforced with polycaprolactone-polylactic acid meshes to create implantable multilayered artificial urethral grafts. In vivo experiments showed that the cell-laden tissue integrated into the urethra with less fibrosis and inflammation compared to its acellular counterpart. Staining to trace the implanted cells confirmed integration into the host organism 3 months postsurgery.</p>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142256959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A hierarchical Bilayered scaffold for periodontal complex structure regeneration","authors":"Wen Qin,&nbsp;Ling Li,&nbsp;Zhao Mu,&nbsp;Weiwei Yu,&nbsp;Yina Zhu,&nbsp;Shuailin Jia,&nbsp;Kun Xuan,&nbsp;Wen Niu,&nbsp;Lina Niu","doi":"10.1002/jbm.a.37793","DOIUrl":"10.1002/jbm.a.37793","url":null,"abstract":"<p>The periodontal tissue comprises alveolar bone, cementum, and periodontal ligament (PDL), forming a highly hierarchical architecture. Although current therapies could regenerate the hard tissue well, the simultaneous reconstruction of hard and soft tissue remains a great clinical challenge with the major difficulty in highly orientated PDL regeneration. Using the unidirectional freeze-casting method and biomimetic mineralization technique, we construct a hierarchical bilayer scaffold with the aligned chitosan scaffold with ZIF-8 resembling PDL, and intrafibrillarly mineralized collagen resembling alveolar bone. The hierarchical bilayer scaffold exhibits different geomorphic clues and chemical microenvironments to realize a perfect simulation of the natural periodontal hierarchical architecture. The aligned scaffold with ZIF-8 could induce the fibrogenic differentiation of bone mesenchymal stromal cells (BMSCs), and the mineralized scaffold could induce osteogenic differentiation of BMSCs. The hierarchical bilayer scaffold could simulate periodontal complex tissue, exhibiting great promise for synchronized multi-tissue regeneration of periodontal tissue.</p>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142157054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Collagen binding and mimetic peptide-functionalized self-assembled peptide hydrogel enhance chondrogenic differentiation of human mesenchymal stem cells","authors":"Günnur Pulat,&nbsp;Oğuzhan Gökmen,&nbsp;Şerife Özcan,&nbsp;Ozan Karaman","doi":"10.1002/jbm.a.37786","DOIUrl":"10.1002/jbm.a.37786","url":null,"abstract":"<p>The avascular structure and low cell migration to the damaged area due to the low number of cells do not allow spontaneous repair of the articular cartilage tissue. Therefore, functional scaffolds obtained from biomaterials are used for the regeneration of cartilage tissue. Here, we functionalized one of the self-assembling peptide (SAP) scaffolds KLD (KLDLKLDLKLDL) with short bioactive motifs, which are the α1 chain of type II collagen binding peptide WYRGRL (C1) and the triple helical collagen mimetic peptide GFOGER (C2) by direct coupling. Our goal was to develop injectable functional SAP hydrogels with proper mechanical characteristics that would improve chondrogenesis. Scanning electron microscopy (SEM) was used to observe the integration of peptide scaffold structure at the molecular level. To assure the stability of SAPs, the rheological characteristics and degradation profile of SAP hydrogels were assessed. The biochemical study of the DNA, glycosaminoglycan (GAG), and collagen content revealed that the developed bioactive SAP hydrogels greatly increased hMSCs proliferation compared with KLD scaffolds. Moreover, the addition of bioactive peptides to KLD dramatically increased the expression levels of important chondrogenic markers such as aggrecan, SOX-9, and collagen Type II as evaluated by real-time polymerase chain reaction (PCR). We showed that hMSC proliferation and chondrogenic differentiation were encouraged by the developed SAP scaffolds. Although the chondrogenic potentials of WYRGRL and GFOGER were previously investigated, no study compares the effect of the two peptides integrated into 3-D SAP hydrogels in chondrogenic differentiation. Our findings imply that these specifically created bioactive peptide scaffolds might help enhance cartilage tissue regeneration.</p>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.a.37786","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142142191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fabrication of anodic and atomic layer deposition-alumina coated titanium implants for effective osteointegration applications","authors":"Pinar Alpaslan Erturk,&nbsp;Sevde Altuntas,&nbsp;Gulseren Irmak,&nbsp;Fatih Buyukserin","doi":"10.1002/jbm.a.37792","DOIUrl":"10.1002/jbm.a.37792","url":null,"abstract":"<p>Biomimicking the chemical, mechanical, and topographical properties of bone on an implant model is crucial to obtain rapid and effective osteointegration, especially for the large-area fractures of the skeletal system. Titanium-based biomaterials are more frequently preferred in clinical use in such cases and coating these materials with oxide layers having chemical/nanotopographic properties to enhance osteointegration and implantation success rates has been studied for a long time. The objective of this study is to examine the high and rapid mineralization potential of anodized aluminum oxide (AAO) coated and atomic layer deposition (ALD)-alumina coated titanium substrates on large deformation areas with difficult spontaneous healing. AAO-coated titanium (AAO@Ti) substrates were fabricated via anodization technique in different electrolytes and their osteogenic potential was analyzed by comparing them to the bare titanium surface as a control. In order to investigate the effect of the ionic characters gained by the surfaces through anodization, the oxidized nanotopographic substrates were additionally coated with an ultrathin alumina layer via ALD (ALD@AAO@Ti), which is a sensitive and conformal coating vapor deposition technique. Besides, a bare titanium sample was also coated with pure alumina by ALD (ALD@Ti) to investigate the effect of nanoscale surface morphology. XPS analysis after ALD coating showed that the ionic character of each surface fabricated by anodization was successfully suppressed. In vitro studies demonstrated that, among the substrates investigated, the mineralization capacity of MG-63 osteosarcoma cells were highest when incubated on ALD-treated and bare AAO@Ti samples that were anodized in phosphoric acid (H₃PO₄_AAO@Ti and ALD@H₃PO₄_AAO@Ti). Mineralization on these substrates also increased consistently beginning from day 2 to day 21. Moreover, immunocytochemistry for osteopontin (OPN) demonstrated the highest expression for ALD@H₃PO₄_AAO@Ti, followed by the H₃PO₄_AAO@Ti sample. Consequently, it was observed that, although ALD treatment improves cellular characteristics on all samples, effective mineralization requires more than a simple ALD coating or the presence of a nanostructured topography. Overall, ALD@H₃PO₄_AAO@Ti substrates can be considered as an implant alternative with its enhanced osteogenic differentiation potential and rapid mineralization capacity.</p>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.a.37792","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142142192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Copper ions-photo dual-crosslinked alginate hydrogel for angiogenesis and osteogenesis","authors":"Guochen Liu,&nbsp;Shanshan Ye,&nbsp;Yue Li,&nbsp;Jing Yang,&nbsp;Simin Wang,&nbsp;Yuan Liu,&nbsp;Sisi Yang,&nbsp;Yinping Tian,&nbsp;Miao Yin,&nbsp;Bo Cheng","doi":"10.1002/jbm.a.37790","DOIUrl":"10.1002/jbm.a.37790","url":null,"abstract":"<p>Early healing of bone defects is still a clinical challenge. Many bone-filling materials have been studied, among which photocrosslinked alginate has received significant attention due to its good biocompatibility and morphological plasticity. Although it has been confirmed that photocrosslinked alginate can be used as an extracellular matrix for 3D cell culture, it lacks osteogenesis-related biological functions. This study constructed a copper ions-photo dual-crosslinked alginate hydrogel scaffold by controlling the copper ion concentration. The scaffolds were shaped by photocrosslinking and then endowed with biological functions by copper ions crosslinking. According to in vitro research, the dual-crosslinked hydrogel increased the compressive strength and favored copper dose-dependent osteoblast differentiation and cell surface adherence of rat bone marrow mesenchymal stem cells and the expression of type I collagen (Col1), runt-related transcription factor 2 (Runx2), osteocalcin (OCN), vascular endothelial growth factor (VEGF). In addition, hydrogel scaffolds were implanted into rat skull defects, and more angiogenesis and osteogenesis could be observed in in vivo studies. The above results show that the copper-photo-crosslinked hydrogel scaffold has excellent osseointegration properties and can potentially promote angiogenesis and early healing of bone defects, providing a reference solution for bone tissue engineering materials.</p>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142127755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}