Journal of biomedical materials research. Part A最新文献

{"title":"Enhanced Biodegradation and Biocompatibility of Vascular Grafts Through Oriented Core-Shell Fibrous Structure and Incorporation of Sodium Tanshinone IIA Sulfonate","authors":"Yunhuan Li,&nbsp;Tao Yang,&nbsp;Kuihua Zhang,&nbsp;Chengyu Zou,&nbsp;Keqing Hou,&nbsp;Anlin Yin","doi":"10.1002/jbm.a.37877","DOIUrl":"10.1002/jbm.a.37877","url":null,"abstract":"<div>\u0000 \u0000 <p>Microstructure and biological activity have been pivotal factors in the modification of vascular grafts. Equally crucial, however, are degradation behavior and mechanical stability, both of which are key to long-term success of grafts. To optimize these properties, we prepared oriented fiber membranes with core-shell structures through coaxial electrospinning, incorporating varying concentrations of sodium tanshinone IIA sulfonate (STS). In this design, poly-ethylene oxide (PEO)/STS served as the core layer, while poly-L-lactide-co-caprolactone (PLCL) formed the shell. Our findings revealed that both random and oriented fiber membranes exhibited excellent mechanical properties. Notably, compared to random fiber membranes, the oriented counterparts showed enhanced hydrophilicity and a tunable degradation rate. Furthermore, the sustained release of STS from the membranes inhibited platelet adhesion and significantly promote cell diffusion, growth, and proliferation. Importantly, the oriented fiber membranes loaded with STS were able to induce a highly organized cell arrangement and upregulate the expression of CD144 and vWF in endothelial cells. These promising findings suggest that oriented core-shell fiber membranes loaded with PEO/STS could offer valuable insights into vascular graft design and hold potential for further exploration in animal studies.</p>\u0000 </div>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 2","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143076682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent Advances in Integrated Organ-Chip Sensing Toward Robust and User-Friendly Systems","authors":"Bryan G. Schellberg,&nbsp;Ryan A. Koppes,&nbsp;Abigail N. Koppes","doi":"10.1002/jbm.a.37876","DOIUrl":"10.1002/jbm.a.37876","url":null,"abstract":"<div>\u0000 \u0000 <p>Organs-on-a-chip (OOC) are an emergent technology that bridge the gap between current in vitro and in vivo models used to inform drug discovery and investigate disease pathophysiology. These systems offer improved bio-relevance and controlled complexity through the integration of physical and/or chemical stimuli matched to physiologically relevant conditions. Although significant advancements have been made toward recreating organ-specific physiology on chip, the methods available to study structure and function of the cell microenvironment are still limited. Established analysis approaches, including fluorescence microscopy, rely on laborious offline workflows that yield limited time-point data. As the OOC field continues to evolve, there is a unique opportunity to engineer improved characterization methods into organ-chip devices. This review provides an overview of current integrated sensing approaches that address current limitations and enable real-time readout of relevant physiological parameters in OOC.</p>\u0000 </div>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 2","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143076742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Myricetin Modulates Matrix Metalloproteinases Expression Induced by TEGDMA in Human Odontoblast–Like Cells","authors":"Paula Alejandra Baldión,&nbsp;Carlos Aldemar Díaz,&nbsp;Diego Enrique Betancourt","doi":"10.1002/jbm.a.37872","DOIUrl":"10.1002/jbm.a.37872","url":null,"abstract":"<div>\u0000 \u0000 <p>The activity of matrix metalloproteinases (MMPs) plays a crucial role in the aging of the resin–dentin interface. The in situ action of MMP-2 and MMP-9 has been confirmed in the process of dentin-collagen degradation. However, the involvement of dental pulp cells in MMP secretion as a response to oxidative stress induced by contact with resin monomers has not been fully elucidated. Myricetin (MYR), like proanthocyanidin (PAC), has antioxidant properties and may help prevent extracellular matrix degradation. The objective was to evaluate the effect of MYR on the MMP expression and activity in response to reactive oxygen species (ROS) increase induced by triethylene glycol dimethacrylate (TEGDMA) in human odontoblast–like cells (hOLCs). hOLCs differentiated from dental pulp mesenchymal stem cells were exposed to TEGDMA released from dentin blocks using a barrier model with transwell inserts for 18, 24, and 36 h. Intracellular oxidation was evaluated using the 2′,7′-dichlorofluorescein probe. The effect of 600 μM MYR on the MMP-2 and MMP-9 expression was determined by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The extracellular MMP levels were quantified using enzyme-linked immunosorbent assay, and their activation by means of a proteolytic fluorometric assay. The results were analyzed by one-way analysis of variance and Tukey's post hoc test, <i>p ≤</i> 0.05. TEGDMA exposure increased intracellular ROS and upregulated MMP-2 and MMP-9 mRNA in hOLCs (<i>p</i> &lt; 0.001). The levels of MMPs increased significantly 24 h after TEGDMA exposure (<i>p</i> = 0.013). These secreted proteases exhibited high activation ability. MYR reduced ROS production and downregulated MMP expression and activity at both mRNA and protein levels, similar to the effect found for PAC, which was used as a control. A relationship was observed between MMP-2 and MMP-9 expression, secretion, and early activation with ROS increase due to TEGDMA exposure. MYR showed potential as a therapeutic strategy to control MMP expression and modulate redox imbalance, offering a protective effect on cellular response.</p>\u0000 </div>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 2","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143076739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Innovative Hybrid Nanocarriers of GnRH Peptide-Modified Chitosan-Coated Lipid Nanoparticles as a Targeted Chemotherapy for Reproductive-Related Cancers","authors":"Phichaporn Bunwatcharaphansakun,&nbsp;Nisakorn Yodsanit,&nbsp;Wisawat Keaswejjareansuk,&nbsp;Pattarapond Gonil,&nbsp;Sudthirak Wongkhieo,&nbsp;Angkana Jantimaporn,&nbsp;Warut Kengkittipat,&nbsp;Somrudee Kaewmalun,&nbsp;Nuttawat Sawangrat,&nbsp;Teerapong Yata,&nbsp;Warayuth Sajomsang,&nbsp;Mattaka Khongkow,&nbsp;Katawut Namdee","doi":"10.1002/jbm.a.37843","DOIUrl":"10.1002/jbm.a.37843","url":null,"abstract":"<div>\u0000 \u0000 <p>Cancer stands as a primary contributor to worldwide mortality, especially reproductive-related cancers (e.g., breast/cervical cancers among females and prostate cancer among males). Chemotherapy is the most common systemic therapy for cancer, but its adverse effects are concerning. Developing effective and new strategies against cancer is necessary to increase their efficacy and minimize their adverse effect. In this work, the novel core-shell structure of lipid nanoparticle (LNP) was fabricated via a high-throughput microfluidic for chemotherapy drug delivery. A gonadotropin-releasing hormone (GnRH), a targeting moiety for the overexpressed GnRH receptors (GnRHR) in cancer cells, was conjugated on chitosan (GnRH-CS) as a shell and used to modify the surface of LNP with doxorubicin loading to form a complex of LNP-dox-GnRH (≤ 150 nm, PDI ~0.2). The modified surface enhances the binding affinity of the LNP to the breast and prostate cancer cells. For an in vitro study, we found that LNP-dox-GnRH can specifically target the GnRHR-overexpressing cancer cells (i.e., MCF-7 and PC-3) compared with non-targeted LNP-dox. Conversely, there was no difference in the targetability between LNP-dox-GnRHR and non-targeted LNP to MDA-MB-436, a low GnRHR-expressing cancer cell. Furthermore, the enhanced anticancer activity of LNP-dox-GnRH was observed in both monolayer and spheroid cell cultures. This study highlights the advantages of easy customization of payloads and targeting peptides, requiring only a simple coating process that doesn't need specialized expertise. Its flexibility and efficiency enhance the potential for precision therapies, making it ideal for translational applications in treating reproductive-related cancers, GnRH-associated diseases, and other conditions.</p>\u0000 </div>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 2","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143082789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polydopamine-Induced BMP7-Poly (Lactic-Co-Glycolic Acid)-Nanoparticle Coating Facilitates Osteogenesis in Porous Tantalum Scaffolds","authors":"Yu Ao,&nbsp;Dianming Jiang","doi":"10.1002/jbm.a.37835","DOIUrl":"10.1002/jbm.a.37835","url":null,"abstract":"<div>\u0000 \u0000 <p>Bone defects are difficult to treat clinically and most often require bone grafting for repair. However, the source of autograft bone is limited, and allograft bone carries the risk of disease transmission and immune rejection. As tissue engineering technology advances, bone replacement materials are playing an increasingly important role in the treatment of bone defects. Porous tantalum (PT) scaffolds have shown beneficial clinical effects in the repair of bone defects, surface modification of PT to induce osteogenic differentiation of mesenchymal stem cells (MSC) is the key to optimizing this material. Poly (lactic-co-glycolic acid) nanoparticle (PLGA NPs) encapsulating bone morphogenetic protein 7 (BMP7) (BPNPs) was prepared by a double emulsion (water/oil/water [W/O/W]) method and adhered on polydopamine (PDA)-coated PT (PPT) that was prepared by biomimetic method to prepare BPNPs-coated PPT (BPPT). The successful preparation of BPPT was monitored by scanning electron microscopy (SEM) and energy spectrum. Murine calvarial preosteoblasts (MC3T3-E1) cells were co-cultured with BPPT, vitro experiments showed that BPPT promoted cell proliferation and osteogenic differentiation. BPPT was further implanted into the bone defect of the distal femoral epiphysis of the rabbit. At 4 weeks postoperatively, in the BPPT group, high-resolution CT reconstruction indicated that bone volume/total volume (BV/TV) was near 50%, and the hard tissue section indicated that the depth of new bone ingrowth into the scaffolds was nearly 2 mm. The immunofluorescence staining of bone tissue around the bone defects indicated that the expression of osteogenic-related proteins was higher in the BPPT group than the other groups. Taken together, our results suggest that BPPT promoted early osteointegration, which may provide a novel approach for the clinical treatment of bone defects.</p>\u0000 </div>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143018676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Promoting Angiogenesis/Osteogenesis by a New Copper/Magnesium Hydroxide Hybrid Nanoparticle: In Vitro and In Vivo Investigation","authors":"Parsa Khalkhali,&nbsp;Meisam Omidi,&nbsp;Daniela S. Masson-Meyers,&nbsp;Babak Akbari,&nbsp;Mohammad Mehdi Dehghan,&nbsp;Hossein Aminianfar,&nbsp;Saeed Farzad-Mohajeri,&nbsp;Vahid Mansouri,&nbsp;Amin Nikpasand,&nbsp;Lobat Tayebi","doi":"10.1002/jbm.a.37855","DOIUrl":"10.1002/jbm.a.37855","url":null,"abstract":"<div>\u0000 \u0000 <p>In this study, a new hybrid nanoparticle composed of magnesium hydroxide and copper oxide (Mg(OH)₂/CuO) with an optimized ratio of magnesium (Mg) to copper (Cu) was designed and incorporated into a 3D-printed scaffold made of polycaprolactone (PCL) and gelatin. These hybrid nanostructures (MCNs) were prepared using a green, solvent-free method. Their topography, surface morphology, and structural properties were characterized using scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS). The fabricated 3D-printed PCL/Gelatin/MCN scaffolds were investigated in vitro and in vivo. Cell viability tests on murine calvarial preosteoblasts (MC3T3-E1) and human umbilical vein endothelial cells (HUVECs) demonstrated that the scaffolds could induce proper cell proliferation. Additionally, the angiogenic and osteogenic properties of the constructs were evaluated using alkaline phosphatase (ALP) activity, osteogenesis-related, and angiogenesis-related gene expression tests. The in vivo study was conducted using a rat calvarial defect model, which confirmed the superior angiogenic and osteogenic properties of the PCL/gelatin/MCN scaffolds compared to PCL/Gelatin and PCL/Gelatin/Mg(OH)₂ scaffolds. Overall, the PCL/Gelatin/MCN scaffolds showed promising potential for bone regeneration, particularly for critical-sized defects where proper angiogenesis is essential for tissue reconstruction.</p>\u0000 </div>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143018837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrically Active Biomaterials for Stimulation and Regeneration in Tissue Engineering","authors":"Jinyoung Park,&nbsp;Gulsah Erel Akbaba,&nbsp;Nidhi Sharma,&nbsp;Ritopa Das,&nbsp;Tra Vinikoor,&nbsp;Yang Liu,&nbsp;Duong Quang Le,&nbsp;Kishan Angadi,&nbsp;Thanh Duc Nguyen","doi":"10.1002/jbm.a.37871","DOIUrl":"10.1002/jbm.a.37871","url":null,"abstract":"<div>\u0000 \u0000 <p>In the human body, bioelectric cues are crucial for tissue stimulation and regeneration. Electrical stimulation (ES) significantly enhances the regeneration of nerves, bones, cardiovascular tissues, and wounds. However, the use of conventional devices with stimulating metal electrodes is invasive and requires external batteries. Consequently, electrically active materials with excellent biocompatibility have attracted attention for their applications in stimulation and regeneration in tissue engineering. To fully exploit the potential of these materials, biocompatibility, operating mechanisms, electrical properties, and even biodegradability should be carefully considered. In this review, we categorize various electrically active biomaterials based on their mechanisms for generating electrical cues, such as piezoelectric effect, triboelectric effect, and others. We also summarize the key material properties, including electrical characteristics and biodegradability, and describe their applications in tissue stimulation and regeneration for nerves, musculoskeletal tissues, and cardiovascular tissues. The electrically active biomaterials hold great potential for advancing the field of tissue engineering and their demonstrated success underscores the importance of continued research in this field.</p>\u0000 </div>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142981074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of Seven Ion Species on Osteogenic Differentiation of Mesenchymal Stem Cells Stimulated by Macrophages in Indirect and Direct Coculture Systems","authors":"Akiko Obata,&nbsp;Makito Iguchi,&nbsp;Hikaru Yasue,&nbsp;Toshihiro Kasuga","doi":"10.1002/jbm.a.37875","DOIUrl":"10.1002/jbm.a.37875","url":null,"abstract":"<div>\u0000 \u0000 <p>Implanted biomaterials release inorganic ions that trigger inflammatory responses, which recruit immune cells whose biochemical signals affect bone tissue regeneration. In this study, we evaluated how mouse macrophages (RAW264, RAW) and mesenchymal stem cells (KUSA-A1, MSCs) respond to seven types of ions (silicon, calcium, magnesium, zinc, strontium, copper, and cobalt) that reportedly stimulate cells related to bone formation. The collagen synthesis, alkaline phosphatase activity, and osteocalcin production of the MSCs varied by ion dose and type after culture in the secretome of RAW cells. However, DNA production was relatively unaffected. The MSC secretome may also stimulate RAW cells in coculture and, therefore, affect osteogenic differentiation of MSCs. Overall, the ions often exerted different effects on each cell type. This study guides future work that explores the mechanisms behind ion-dependent osteogenic differentiation and cell functions.</p>\u0000 </div>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142981077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Streamlined High-Throughput LC–MS Assay for Quantifying Peptide Degradation in Cell Culture","authors":"Samuel J. Rozans,&nbsp;Yingjie Wu,&nbsp;Abolfazl S. Moghaddam,&nbsp;E. Thomas Pashuck","doi":"10.1002/jbm.a.37864","DOIUrl":"10.1002/jbm.a.37864","url":null,"abstract":"<p>Peptides are widely used in biomaterials due to their ease of synthesis, ability to signal cells, and modify the properties of biomaterials. A key benefit of using peptides is that they are natural substrates for cell-secreted enzymes, which creates the possibility of utilizing cell-secreted enzymes for tuning cell–material interactions. However, these enzymes can also induce unwanted degradation of bioactive peptides in biomaterials, or in peptide therapies. Liquid chromatography–mass spectrometry (LC–MS) is a widely used, powerful methodology that can separate complex mixtures of molecules and quantify numerous analytes within a single run. There are several challenges in using LC–MS for the multiplexed quantification of cell-induced peptide degradation, including the need for nondegradable internal standards and the identification of optimal sample storage conditions. Another problem is that cell culture media and biological samples typically contain both proteins and lipids that can accumulate on chromatography columns and degrade their performance. Removing these constituents can be expensive, time-consuming, and increases sample variability. However, loading unpurified samples onto the column without removing lipids and proteins will foul the column. Here, we show that directly injecting complex, unpurified samples onto the LC–MS without any purification enables rapid and accurate quantification of peptide concentration and that hundreds of LC–MS runs can be done on a single column without significantly diminishing the ability to quantify the degradation of peptide libraries. To understand how repeated injections degrade column performance, a model library was injected into the LC–MS hundreds of times. It was then determined that column failure is evident when hydrophilic peptides are no longer retained on the column and that failure can be easily identified by using standard peptide mixtures for column benchmarking. In total, this work introduces a simple and effective method for simultaneously quantifying the degradation of dozens of peptides in cell culture. By providing a streamlined and cost-effective method for the direct quantification of peptide degradation in complex biological samples, this work enables more efficient assessment of peptide stability and functionality, facilitating the development of advanced biomaterials and peptide-based therapies.</p>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.a.37864","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142981071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Bioprinted Hydrogel Patch With Bioactive Glass: A New Frontier in Chronic Wound Healing","authors":"T. Petrachi,&nbsp;A. Portone,&nbsp;D. Bellucci,&nbsp;L. Pacchioni,&nbsp;C. Marra,&nbsp;G. De Santis,&nbsp;L. Rovati,&nbsp;M. Dominici,&nbsp;E. Veronesi,&nbsp;V. Cannillo","doi":"10.1002/jbm.a.37865","DOIUrl":"10.1002/jbm.a.37865","url":null,"abstract":"<p>A wound, defined as a disruption in the continuity of the skin, is among the most common issues in the population and poses a significant burden on healthcare systems and economies worldwide. Despite the countless medical devices currently available to promote wound repair and skin regeneration, there is a growing demand for new skin devices that incorporate innovative biomaterials and advanced technologies. Bioglasses are biocompatible and bioactive materials capable of interacting with biological tissues. Due to their ability to promote fibroblast proliferation, angiogenesis, collagen production, and evade antibacterial activity, they have been suggested as key players in the skin regeneration process. Since their initial introduction, various compositions have been proposed depending on the clinical goal to be achieved. Recently, a novel bioglass composition named Bio_MS was found to exhibit significant bone regenerative potential. Given its peculiar composition characterized by strontium and magnesium, Bio_MS could also play a role in skin healing. In the present work, an innovative patch was designed by combining the attractive characteristics of Bio_MS with bioprinting technology. The regenerative potential of the Bio_MS patch was tested in an ex vivo cutaneous model using human skin in which an experimental wound was induced by sodium dodecyl sulfate incubation. After injury, the Bio_MS patch was able to restore skin architecture and enhance the epidermal barrier function. Additionally, the Bio_MS patch demonstrated therapeutic effects in both the epidermis and dermis, making it suitable not only for superficial lesions but also for deep wounds.</p>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.a.37865","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142967664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}