Journal of biomedical materials research. Part A最新文献

{"title":"Field-Emitted Silver Ions at Atmospheric Pressure: Antibacterial Activity and Penetration Into Artificial Skin","authors":"Yusuke Daiko,&nbsp;Mayuka Akiyama,&nbsp;Kenta Matsuoka,&nbsp;Daisuke Urushihara,&nbsp;Akiko Obata","doi":"10.1002/jbm.a.37995","DOIUrl":"10.1002/jbm.a.37995","url":null,"abstract":"<p>Silver (Ag⁺) ions are field-emitted under atmospheric pressure from a sharpened Ag⁺ ion-conductive glass by applying a high voltage. This study investigates the antibacterial efficacy of emitted Ag⁺ ions. When Ag⁺ ions are irradiated onto hydroxyapatite (HAP) for 5 min, an antibacterial effect against <i>Escherichia coli</i> is clearly observed. Furthermore, Ag⁺ ion irradiation directly into the <i>E. coli</i> suspension results in a significant reduction in viable <i>E. coli</i> after 24 h of incubation, compared to immediately after ion irradiation. Although Ag⁺ ions are expected to rapidly lose energy upon collision with air molecules, penetration exceeding 100 μm into the hydrated agar gel is confirmed. When Ag⁺ ions are irradiated onto the surface of an artificial skin (3D reconstructed human epidermis model), fungal cells located beneath the skin are successfully eliminated. These results demonstrate, for the first time, that field-emitted Ag⁺ ions under atmospheric conditions exhibit potent antimicrobial activity.</p>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 10","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.a.37995","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145139324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Carrier-Free Nanomaterials Simultaneously Combat Infection and Inflammation for Enhanced Diabetic Wound Healing","authors":"Ke Wang,&nbsp;Jue Zhang,&nbsp;Bing Han,&nbsp;Youyou Xu,&nbsp;Jiayue Xu,&nbsp;Fuhong Yuan,&nbsp;Lei Wang,&nbsp;Jianwei Zhu","doi":"10.1002/jbm.a.37996","DOIUrl":"https://doi.org/10.1002/jbm.a.37996","url":null,"abstract":"<div>\u0000 \u0000 <p>Diabetic wound ulcers, characterized by chronic infection and persistent inflammation, significantly impair patient quality of life and present substantial socioeconomic burdens. Traditional therapeutic strategies frequently encounter challenges, including antibiotic resistance, systemic side effects, and inadequate control over localized inflammation. We herein developed novel carrier-free nanoparticles (CeCur NPs) by self-assembling chlorin e6 (Ce6) and curcumin, achieving simultaneous photodynamic antibacterial activity and sustained anti-inflammatory effects. CeCur NPs exhibited effective and synergetic anti-bacteria and anti-inflammation both in vitro and in vivo. Comprehensive biosafety evaluations further confirmed the excellent biocompatibility of CeCur NPs, underscoring their potential as a promising therapeutic strategy for accelerating diabetic wound healing.</p>\u0000 </div>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 10","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145146760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antifungal Activity, Cytocompatibility, and Wound Healing Potential of Novel Mucoadhesive Formulations for Oral Drug Delivery","authors":"Carolina Yoshi Campos Sugio,&nbsp;Victor Martin,&nbsp;Lídia Maria Diogo Gonçalves,&nbsp;Priscileila Coleto Ferrari,&nbsp;Vanessa Migliorini Urban,&nbsp;Karin Hermana Neppelenbroek,&nbsp;Maria Helena Fernandes","doi":"10.1002/jbm.a.37990","DOIUrl":"https://doi.org/10.1002/jbm.a.37990","url":null,"abstract":"<p>Conventional treatments for oral candidiasis often fail due to the complexities of the oral environment and the increasing antifungal drug resistance. Therefore, there is a growing demand for new therapies that optimize drug bioavailability, allowing for lower therapeutic doses while enhancing cytocompatibility, maintaining antifungal, anti-inflammatory, and wound healing efficacy. This study investigated the antifungal activity, cytocompatibility, wound healing potential, and mucosal adhesion of novel mucoadhesive formulations containing nystatin (NYS) or chlorhexidine (CHX) complexed with β-cyclodextrin (βCD), compared with the drug-free formulation (GEL) and the standard treatment with 2% miconazole gel (DK—Daktarin). Efficacy against <i>Candida albicans</i> was evaluated by measuring the metabolic activity, whereas cytocompatibility with human gingival fibroblasts (HGFs) was analyzed for viability, morphology, lactate dehydrogenase (LDH) release, and apoptosis. Additionally, wound healing potential was investigated by assessing cell migration efficacy, anti-inflammatory activity, and reactive oxygen species (ROS) scavenging activity. Mucoadhesion was evaluated using mucin discs and a texture analyzer. Mucoadhesive gels containing βCD-complexed NYS or CHX exhibited significantly higher antifungal activity when compared to the GEL and DK groups (<i>p</i> &lt; 0.05). Compared to fibroblast control cultures, those exposed to drug-complexed gels exhibited similar viability (<i>p</i> &gt; 0.05) and morphological parameters, lower LDH release (<i>p</i> &lt; 0.05), and similar apoptosis rates (<i>p</i> &gt; 0.05). Additionally, exposure to the βCD-modified gels was associated with complete wound closure (<i>p</i> &gt; 0.05), significant anti-inflammatory effect, with downregulation of pro-inflammatory gene expression (<i>p</i> &lt; 0.05), and higher ROS scavenging activity (<i>p</i> &lt; 0.05). The developed formulations showed no difference in mucoadhesiveness (<i>p</i> &gt; 0.05), which was superior to that of DK (<i>p</i> &lt; 0.05). Therefore, the proposed drug-complexed mucoadhesives are promising therapeutic options for oral candidiasis.</p>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 10","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.a.37990","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145110665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantitative Analysis of Glutathione Utilizing Highly Luminescent Gold Nanoclusters","authors":"Wen He,&nbsp;Yanping Qu,&nbsp;Shukai Wu,&nbsp;Soh Fong Lim,&nbsp;Ling Mo,&nbsp;Hongyu Mou,&nbsp;Jibin Song","doi":"10.1002/jbm.a.37988","DOIUrl":"https://doi.org/10.1002/jbm.a.37988","url":null,"abstract":"<div>\u0000 \u0000 <p>Glutathione (GSH), a pivotal regulator of cellular redox homeostasis, requires precise monitoring for clinical diagnostics. This work develops bovine serum albumin-templated gold nanoclusters (BSA-AuNCs) through in situ chloroauric acid reduction, exhibiting orange fluorescence (<i>λ</i>_em = 610 nm) with 35.8% quantum yield. Systematic evaluation of pH-dependent and time-resolved quenching behavior revealed optimal GSH detection at physiological pH 7.4 within 3 min. The nanozyme demonstrated linear responsivity from 10 nM to 3 μM (Limit of Detection, LOD = 8.7 nM) and validated applicability in biological matrices (urine/serum) with 98.0%–103.3% recovery rates. This photoluminescent platform enables reliable GSH biomarker quantification, advancing point-of-care testing for oxidative stress disorders.</p>\u0000 </div>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 10","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145110897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanochromic Polyurethane Shape Memory Polymer for Biomedical Applications","authors":"Thalma Orado,&nbsp;Bethany Yashkus,&nbsp;Richard Chandardat,&nbsp;Samantha Zysk,&nbsp;Zachary J. Geffert,&nbsp;Ernest Emmanuel Obeng,&nbsp;Xiaocun Lu,&nbsp;Pranav Soman,&nbsp;Mary Beth B. Monroe","doi":"10.1002/jbm.a.37979","DOIUrl":"https://doi.org/10.1002/jbm.a.37979","url":null,"abstract":"<p>The incorporation of functional molecular switches into smart materials imparts dynamic material properties, gaining deeper insight into how molecular structure affects the functionality of these materials and aiding the development of novel sensor devices. To enable mechanochromic biomaterials capable of sensing shape changes, we explored the incorporation of spiropyran (SP) mechanophores into a polyurethane (PUR) shape memory polymer (SMP). SPs reversibly generate variations in fluorescence and visual colors due to conversion from inactivated SP to activated merocyanine (MC) in response to force. We hypothesized that SP-containing PUR (PUR-SP) could undergo simultaneous shape and color changes. Small quantities of SP were dissolved in control PUR solutions with different hard-to-soft segment ratios, and PUR-SP films were formed by solvent-casting. The effect of SP incorporation on material properties, including mechanical, shape memory, thermal, and cytocompatibility, was studied. Mechanochromic behavior was analyzed by straining the films and imaging using a camera and fluorescence microscopy. We also employed a previously developed bacterial protease-responsive PUR SMP to confirm that SP incorporation enables simultaneous shape and color changes in the presence of bacteria. Strained samples showed increased fluorescence (up to 56%, <i>p</i> &lt; 0.05), which was reversed upon shape recovery. Mechanochromic behavior was affected by the hard-to-soft segment ratio of the PUR, SP concentration, and strain percentage. Bacteria-responsive PURs with SP showed reduction in fluorescence and complete biofilm removal after incubation with <i>Staphylococcus</i> <i>aureus</i> for 24 h, which conveyed the potential to use SP in PURs as a molecular force probe with color-based bacteria detection. This technology could be expanded to include a range of other stimuli-responsive functionalities in future work to enable shape and color changes based on environmental cues.</p>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 10","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.a.37979","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145110841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In Vitro Characterization of Biodegradable Polyurethane Foams With Facile Gelatin Modification for Traumatic Wound Hemostasis and Regeneration","authors":"Natalie Marie Petryk,&nbsp;Mary Beth B. Monroe","doi":"10.1002/jbm.a.37982","DOIUrl":"https://doi.org/10.1002/jbm.a.37982","url":null,"abstract":"<p>Polyurethane (PUr) foams are widely explored for embolic, hemostatic, and tissue engineering applications. Their tunable pore structure, mechanical properties, and degradation rates make PUr foams ideal scaffolds for thrombus formation and cell infiltration. Despite their embolic and hemostatic efficacy, PUrs are entirely synthetic, which limits their long-term healing capacity to facilitate tissue regeneration. To improve PUr-driven healing, this work explores the facile modification of biodegradable PUr foams with bioactive gelatin through simple physical and chemical incorporation methods accomplished post-foam fabrication. The gelatin-modified PUr foams had increased platelet interactions and quicker clotting times than the unmodified PUr foams due to the procoagulant nature of gelatin. Furthermore, the gelatin-modified foams had significantly improved cell attachment, spreading, and proliferation of fibroblasts on foam pores, which could translate to enhanced wound repair through tissue migration into the PUr scaffold. Overall, the simple modification of biodegradable PUr foams with bioactive gelatin can significantly improve healing outcomes in traumatic wounds and various regenerative tissue applications.</p>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 10","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.a.37982","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145110849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Submucosal Hydrogel for Spring-Mediated Intestinal Lengthening","authors":"Fereshteh Salimi-Jazi,&nbsp;Narelli de Paiva Narciso,&nbsp;Gillian Fell,&nbsp;Anne-Laure Thomas,&nbsp;Renato S. Navarro,&nbsp;Talha Rafeeqi,&nbsp;Neil J. Baugh,&nbsp;Riley A. Suhar,&nbsp;Julie-Ann Nguyen,&nbsp;Nolan Lopez,&nbsp;Sarah C. Heilshorn,&nbsp;James C. Y. Dunn","doi":"10.1002/jbm.a.37986","DOIUrl":"https://doi.org/10.1002/jbm.a.37986","url":null,"abstract":"<div>\u0000 \u0000 <p>Spring-mediated distraction enterogenesis has shown success in intestinal lengthening, with spring confinement achieved by external plication with sutures to reduce the lumen diameter at both ends of the intestinal segment. Endoscopic spring placement would minimize the morbidity associated with device insertion. This study investigates the use of submucosal injection of engineered hydrogel to temporarily confine a compressed spring within an intestinal segment. Engineered hydrogels were composed of hyaluronic acid (HA) alone or HA with elastin-like protein (HELP). To simulate endoscopic injection in six juvenile pigs, hydrogel was injected into the submucosa in everted jejunum, followed by the placement of a gelatin-encapsulated, compressed nitinol spring. The jejunum was then unfolded over the spring, and hydrogel was injected distally into the submucosa. Sutures were placed as fiducial markers. After 7 days on a liquid diet, the pigs were euthanized, and their intestinal segments were analyzed for lengthening and histological changes. The spring-containing jejunal segments expanded in all animals, lengthening to 132% in the HA group and 188% in the HELP group. HELP hydrogels exhibited slower biodegradation than HA-only hydrogels. Histological analysis showed increased crypt width and decreased crypt density in the spring-containing segments compared to controls. Hydrogel effectively provides temporary spring confinement within intestinal segments without adverse effects. The mechanical stimulation from the spring induces crypt fission, expanding the intestinal epithelium. These results support the feasibility of gel-enabled, spring-mediated distraction enterogenesis for intestinal lengthening.</p>\u0000 </div>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 10","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145110901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hyaluronic Acid/Type I Collagen Hydrogels With Tunable Physicochemical Properties Using Diels–Alder Click Chemistry","authors":"Rabia Fatima,&nbsp;Bethany Almeida","doi":"10.1002/jbm.a.37992","DOIUrl":"https://doi.org/10.1002/jbm.a.37992","url":null,"abstract":"<div>\u0000 \u0000 <p>Hydrogels that combine mechanical tunability with biochemical relevance are essential for engineering tissue-mimetic scaffolds for tissue engineering and regenerative medicine applications. In this study, we present for the first time a tunable hydrogel platform formed via Diels–Alder bioorthogonal click chemistry using furan-functionalized hyaluronic acid (HA-furan), furan-functionalized type I collagen (Col-furan), and bis-maleimide-functionalized polyethylene glycol (mal-PEG-mal). Hydrogels were fabricated at furan:maleimide molar ratios of 1:0.5, 1:1, and 1:2.5 and gelled under physiological conditions for 24 h without the need for catalysts or initiators. Material characterization revealed that this mechanism fabricated predominantly elastic hydrogels, where the 1:1 M ratio hydrogel was the most stable and had the highest mechanical properties, with a Young's modulus that was 2.1-fold and 4.7-fold larger than the 1:0.5 and 1:2.5 M ratio hydrogels, respectively. Further analysis revealed that hydrogel stability and performance were predominantly controlled by hydrogel structure (amorphous vs. crystalline) and crosslinking density. This enhanced mechanical stability and performance were also synergized with enhanced bioactivity from the incorporation of Col, which introduced native Arg-Gly-Asp (RGD) motifs that support cell interactions. Overall, this bioactive yet biomechanically stable hydrogel system provides a tunable platform for engineering extracellular matrix-inspired biomaterials with broad potential for soft tissue repair and regenerative medicine applications.</p>\u0000 </div>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 10","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145110898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cobalt-Doped Monetite-Induced Biomimetic Hypoxia Camouflages Osteogenic Healing Microenvironment","authors":"Gerson Santos de Almeida,&nbsp;Thais Silva Pinto,&nbsp;Luísa Camilo Suter,&nbsp;Geórgia da Silva Feltran,&nbsp;Maria Gabriela Jacheto Carra,&nbsp;Julia Ferreira Moraes,&nbsp;Diego Rafael Nespeque Corrêa,&nbsp;Paulo Noronha Lisboa Filho,&nbsp;Margarida Juri Saeki,&nbsp;Willian Fernando Zambuzzi","doi":"10.1002/jbm.a.37984","DOIUrl":"https://doi.org/10.1002/jbm.a.37984","url":null,"abstract":"<div>\u0000 \u0000 <p>To address developing novel biomimetic material able to camouflage osteogenic healing microenvironment, this study looked to synthesize and characterize a cobalt-doped monetite (CoCaP). After synthesizing, the samples were subjected to physicochemical and biological characterization a comprehensive structural analysis encompassing a suite of complementary techniques. Previously, our data show a validation and reveal distinct structural alterations from cobalt doping. Biologically, Co-doped monetite had no cytotoxic effects on osteoblasts up to 7 days; rather, it contributed to osteoblast adhesion and migration, here estimated by carrying out a wound healing assay. Thereafter, we have linked this phenomenon to an upregulation of cyclin-dependent kinases (CDKs) genes, and it was hypothesized to be related to the dynamic adhesion-related machinery requiring the upregulation of integrins, focal adhesion kinase (FAK), and Src. Complementarily, osteoblast differentiation was also investigated, and our data clearly show a strong stimulus of osteogenic phenotype, once it was shown a significantly increased upregulation of both classical osteogenic transcription factors Runx2 and Osterix, both in response to Co-doped monetite. Additionally, we observed extracellular matrix (ECM) remodeling requiring the activities of matrix metalloproteinase 9 (MMP9) zymogens, suggesting effective collagen turnover along osteoblast differentiation and mineralization. Collectively, our findings show the biological impact of Co-doped monetite on the osteogenic phenotype of pre-osteoblasts. Notably, cobalt-doped monetite induces biomimetic hypoxia, and it recapitulates relevance on the osteogenic phenotype required for the bone healing microenvironment. Thus, Co-doped monetite emerges as a biomimetic and “smart” advanced material for promising applications in bone injuries or the bioactive surface of dental implants in the future.</p>\u0000 </div>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 10","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145110842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanostructured Polyurethane-Collagen Hydrogels: Bioactive Crosslinked Networks for Enhanced Wound Healing","authors":"María I. León-Campos,&nbsp;Jesús A. Claudio-Rizo,&nbsp;Luis E. Cobos-Puc,&nbsp;Denis A. Cabrera-Munguía,&nbsp;Anilú Rubio-Rios,&nbsp;Ernesto Oyervides-Muñoz,&nbsp;Angélica Velázquez-Arrellano","doi":"10.1002/jbm.a.37993","DOIUrl":"https://doi.org/10.1002/jbm.a.37993","url":null,"abstract":"<div>\u0000 \u0000 <p>Nanostructured polyurethanes (nPUs) are promising materials for biomedical applications due to their mechanical strength, controlled degradation, and bioactivity. In this study, collagen-based hydrogels were developed using nPUs synthesized from ethoxylated glycerol and either hexamethylene diisocyanate (HDI) or isophorone diisocyanate (IPDI), functionalized with L-tyrosine (T). These nPUs were incorporated at 15% and 30% by weight into porcine dermis collagen. The HDI-based nPUs (HDI-T), with particle sizes between 6 and 58 nm, achieved high crosslinking densities (&gt; 90%) and superabsorbent capacities (&gt; 6000%), which accelerated gelation under physiological conditions. The resulting hydrogels showed enhanced elasticity and resistance to deformation—critical for wound healing. Structural analysis revealed semi-crystalline and rough surfaces. Hydrogels crosslinked with HDI-T (P(HDI-T)) exhibited excellent hydrolytic stability at pH 8.5 and in simulated body fluids (SBF), as well as reduced enzymatic degradation. These systems allowed for sustained release of methylene blue at both physiological and acidic pH, while ketorolac release was more pronounced in acidic conditions. Biologically, the hydrogels were non-hemolytic and biocompatible, promoting monocyte and fibroblast metabolic activity. Notably, P(HDI-T30) hydrogels stimulated the release of Interleukin-10 (IL-10), contributing to inflammation modulation. In addition, they exhibited potent antibacterial activity, inhibiting <i>Escherichia coli (E. coli)</i> growth by up to 150% and <i>Staphylococcus aureus (S. aureus)</i> by 60% compared to controls. In vivo, complete wound closure was observed by Day 17, with regenerated tissue rich in collagen. These findings demonstrate the potential of nPU–collagen hydrogels as multifunctional biomaterials for advanced wound healing, combining mechanical integrity, controlled drug release, antibacterial efficacy, and immune modulation.</p>\u0000 </div>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 10","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145110899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}