Journal of biomedical materials research. Part A最新文献

{"title":"Investigating the Potential of Magnesium Microparticles on Cartilage and Bone Regeneration Utilizing an In Vitro Osteoarthritis Model","authors":"Bavya Mavila Chathoth,&nbsp;Heike Helmholz,&nbsp;Nina Angrisani,&nbsp;Björn Wiese,&nbsp;Janin Reifenrath,&nbsp;Regine Willumeit-Römer","doi":"10.1002/jbm.a.37862","DOIUrl":"10.1002/jbm.a.37862","url":null,"abstract":"<p>Osteoarthritis (OA) is a significant condition that profoundly impacts synovial joints, including cartilage and subchondral bone plate. Biomaterials that can impede OA progression are a promising alternative or supplement to anti-inflammatory and surgical interventions. Magnesium (Mg) alloys known for bone regeneration potential were assessed in the form of Mg microparticles regarding their impact on tissue regeneration and prevention of OA progression. In vitro assays based on mesenchymal stem cells (SCP-1) were applied to evaluate the Mg microparticle's compatibility and function. Biocompatibility documented through live-dead staining and lactate dehydrogenase assay revealed a 90% cell viability at a concentration below 10 mM after 3 days of exposure. An in vitro OA model based on the supplementation of the cytokines IL-1β, and TNF-α was established and disclosed the effect of Mg degradation products in differentiating SCP-1 cells. Sustained differentiation was confirmed through extracellular matrix staining and increased gene marker expression. The Mg supplementation reduced the release of inflammatory cytokines (IL-6 and IL-8) while promoting the expression of proteins such as collagen X, collagen I, and osteopontin in a time-dependent manner. The in vitro study suggests that Mg microparticles hold a therapeutic potential for OA treatment with their ability to support bone and cartilage repair mechanisms even under inflammatory conditions.</p>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.a.37862","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142886327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual-Polymer Carboxymethyl Cellulose and Poly(Ethylene Oxide)-Based Gels for the Prevention of Postsurgical Adhesions","authors":"Anisha A. DSouza,&nbsp;Mansoor M. Amiji","doi":"10.1002/jbm.a.37852","DOIUrl":"10.1002/jbm.a.37852","url":null,"abstract":"<div>\u0000 \u0000 <p>Postsurgical adhesions are a common complication associated with surgical procedures; they not only impact the patient's well-being but also impose a financial burden due to medical expenses required for reoperative surgeries or adhesiolysis. Adhesions can range from a filmy, fibrinous, or fibrous vascular band to a cohesive attachment, and they can form in diverse anatomical locations such as the peritoneum, pericardium, endometrium, tendons, synovium, and epidural and pleural spaces. Numerous strategies have been explored to minimize the occurrence of postsurgical adhesions. These strategies include surgical approaches, adhesiolysis, antiadhesive agents, and mechanical barriers which have demonstrated the most promise in terms of efficacy and breadth of indications. In this review, we discuss the use of physical/mechanical barriers for adhesion prevention and outline the most commonly used, commercially available barriers. We then focus on a synthetic, dual-polymer gel composed of carboxymethyl cellulose (CMC) and poly(ethylene oxide) [PEO], which, unlike the more commonly used single-polymer hydrogels, has demonstrated higher efficacy across a greater range of indications and surgical procedures. We review the formulation, mechanical properties, and mechanisms of action of the CMC + PEO dual-polymer gel and summarize findings from clinical studies that have assessed the efficacy of CMC + PEO gels in multiple surgical settings in clinics across the world. In conclusion, the CMC + PEO dual-polymer gel represents an approach to preventing postsurgical adhesions that has been commonly used over the last 20 years and could therefore serve as a foundation for research into improving postsurgical outcomes as well as a drug delivery device to expand the use of gels in surgical settings.</p>\u0000 </div>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142886323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nitrided Ti-6Al-4V: A Catalyst for Increase Mineralization and Osteogenic Marker Expression","authors":"Annop Krasaesin,&nbsp;Suttiporn Pinijsuwan,&nbsp;Chatdanai Boonruang,&nbsp;Kanokwan Sriwattanapong,&nbsp;Thantrira Porntaveetus,&nbsp;Thanaphum Osathanon,&nbsp;Shuichi Watanabe,&nbsp;Chavin Jongwannasiri,&nbsp;Chawan Manaspon","doi":"10.1002/jbm.a.37853","DOIUrl":"10.1002/jbm.a.37853","url":null,"abstract":"<div>\u0000 \u0000 <p>Plasma nitriding is one of the surface modifications that show more effectiveness than other methods. In this study, the plasma-based ion implantation (PBII) technique was performed on the surface of titanium alloy (Ti-6Al-4V, Ti64) using a mixture of nitrogen (N₂) and argon (Ar), resulting in a plasma-nitrided surface (TiN-Ti64). The surface composition of the TiN-Ti64 was verified through X-ray photoelectron spectroscopy (XPS). TiN-Ti64 demonstrated superior hydrophilicity compared with Ti64. TiN-Ti64 exhibited higher surface hardness than the original surface. The biological responses of primary human alveolar bone cells (hAVs) were observed on the TiN-Ti64, revealing greater activation of cell adhesion and spreading compared with Ti64 and the control group (glass coverslip). Moreover, the TiN-Ti64 significantly promoted cell proliferation compared with Ti64 and tissue culture plates. The mineralization of hAVs on the TiN-Ti64 showed a significant increase, almost 20% greater than that of Ti64. Furthermore, a significant upregulation of mRNA expression for osteogenic differentiation marker genes, including <i>BMP2</i>, <i>OCN</i>, <i>OPN</i>, and <i>RUNX2</i>, was observed in TiN-Ti64 compared with other conditions. In addition, the TiN-Ti64 exhibited antibiofilm activity against <i>Streptococcus aureus.</i> In conclusion, the TiN-Ti64, modified with the PBII technique utilizing a mixture of N₂ and Ar, emerges as a promising alternative for surface modification in dental implant applications.</p>\u0000 </div>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2024-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142873706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Soluble Proteins From Conventional and Organic Eggshell Membranes With Different Proteomic Profiles Show Similar In Vitro Biofunctions","authors":"Qianli Ma,&nbsp;Lya Piaia,&nbsp;Dagnija Loca,&nbsp;Kristaps Rubenis,&nbsp;Janis Locs,&nbsp;Bernd Thiede,&nbsp;Ólafur Eysteinn Sigurjónsson,&nbsp;Håvard Jostein Haugen","doi":"10.1002/jbm.a.37848","DOIUrl":"10.1002/jbm.a.37848","url":null,"abstract":"<p>The eggshell membrane (ESM), resembling the extracellular matrix (ECM), acts as a protective barrier against bacterial invasion and offers various biofunctions due to its porous structure and protein-rich composition, such as ovalbumin, ovotransferrin, collagen, soluble protein, and antimicrobial proteins. However, the structure of ESM primarily comprises disulfide bonds and heterochains, which poses a challenge for protein solubilization/extraction. Therefore, the method of dissolving and extracting bioactive protein components from ESM has significant potential value and importance for exploring the reuse of egg waste and environmental protection. In this study, soluble ESM proteins (SEPs) were extracted from conventional (industrial-fed) and organic (free-grounded) using an acidic 3-mercaptopropionic acid (3-MPA) extraction strategy. FTIR was employed to monitor the chemical changes in the ESM, while LC–MS/MS was used to conduct the proteomic analysis. The biocompatibility and effects of SEP cocktails on ECM synthesis were also investigated. The results indicated that the acidic 3-MPA strategy effectively altered the ESM chemical composition, thereby facilitating SEPs extraction. The SEPs from conventional and organic eggs have different protein profiles but with partial overlapping. SEPs from both sources showed similar desirable biosafety profiles and dose-dependent promotion of osteoblastic (ECM) component synthesis, suggesting that different egg sources may contribute to consistent core biological functions of protein products, they may also introduce different functional priorities.</p>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.a.37848","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142848463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dextran Sulfate-Modified and pH-Responsive Nanoprobes for Magnetic Resonance/Fluorescence Dual-Modality Imaging of Vulnerable Plaques","authors":"Jianing Cheng,&nbsp;Liguo Hao,&nbsp;Xiaorong Zhu,&nbsp;Ruifan Ma,&nbsp;Silong Li,&nbsp;Qiangqiang Yin,&nbsp;Dongxu Wang,&nbsp;Haifeng Hu,&nbsp;Tianyu Zhang,&nbsp;Zheng Li,&nbsp;Houyi Cong,&nbsp;Xiaoyang Zheng,&nbsp;Jun He,&nbsp;Yuguang Wang","doi":"10.1002/jbm.a.37847","DOIUrl":"10.1002/jbm.a.37847","url":null,"abstract":"<div>\u0000 \u0000 <p>Triggered by the vulnerability to atherosclerotic plaques, cardiovascular diseases (CVDs) have become a main reason for high mortality worldwide. Thus, there is an urgent need to develop functional molecular imaging modalities to improve the detection rate of vulnerable plaques. In this study, polyethyleneimine (PEI) was coated on the surface of mesoporous silica nanoprobes (MSN) loaded with Gd₂O₃ (MSN@Gd₂O₃), followed by coupling the fluorescent dye carboxylated heptamethine cyanine (IR808), and then the dextran sulfate (DS) was modified on the surface of MSN@Gd₂O₃@IR808 by electrostatic adsorption, to construct a targeted and pH-responsive magnetic resonance (MR)/near-infrared fluorescence imaging (NIRF) dual-modal nanoprobe (MSN@Gd₂O₃@IR808@DS nanoparticles). The nanoprobe presented a more concentrated distribution of spherical shapes in transmission electron microscopy. In vitro simulated vulnerable plaque microenvironment (pH = 5.5) presented significant T₁-weighted imaging (T₁WI) signal and longitudinal relaxation in the nanoprobe. Immunofluorescence staining and cellular uptake assays showed that MSN@Gd₂O₃@IR808@DS nanoparticles have the ability to specially bind to scavenger receptors A (SR-A). In vascular endothelium from the high-fat diet (HFD) New Zealand White rabbits, MSN@Gd₂O₃@IR808@DS nanoparticles can exhibit specific contrast-enhanced signals by MR/NIRF dual-modal imaging. In addition, cytotoxicity assays and hematoxylin and eosin (H&amp;E) staining results demonstrated that MSN@Gd₂O₃@IR808@DS nanoparticles have good biocompatibility. Hence, this multifunctional MR/NIRF bimodal nanoprobe provides new experimental and technological ideas for the accurate diagnosis of vulnerable plaques.</p>\u0000 </div>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142820482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Injectable Nano-Micron AKBA Delivery Platform for Treatment of Tendinopathy in a Rat Model","authors":"Qibin Han,&nbsp;Yinhua Qian,&nbsp;Lang Bai,&nbsp;Jing Zhou,&nbsp;Yuefeng Hao,&nbsp;Dan Hu,&nbsp;Zhouzhou Zhang,&nbsp;Xing Yang","doi":"10.1002/jbm.a.37844","DOIUrl":"10.1002/jbm.a.37844","url":null,"abstract":"<div>\u0000 \u0000 <p>Tendinopathy is a disorder characterized by pain and reduced function due to a series of changes in injured or diseased tendons. Inflammation and collagen degeneration are key contributors to the onset and chronic nature of tendinopathy. Acetyl-11-keto-β-boswellic acid (AKBA) is an effective anti-inflammatory agent widely used in chronic inflammatory disorders and holds potential for tendinopathy treatment; however, its therapeutic efficacy is limited by poor aqueous solubility. Here, we fabricated AKBA-encapsulated cationic liposome-gelatin methacrylamide (GelMA) microspheres (GM-Lipo-AKBA) using thin-film hydration and microfluidic technology for drug delivery therapy. GM-Lipo-AKBA exhibited high encapsulation efficiency, extended AKBA release for over 4 weeks, and prolonged degradation. In vitro and in vivo experiments demonstrated its effectiveness in improving inflammation and ECM remodeling in tendinopathy. In summary, the injectable nano-micron drug delivery platform provides a promising strategy for the sustained and localized delivery of AKBA for tendinopathy treatment.</p>\u0000 </div>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142820488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Gamma Irradiation on Structural, Chemical, Bioactivity and Biocompatibility Characteristics of Bioactive Glass–Polymer Composite Film","authors":"Mamun Khan Sujon,&nbsp;Siti Fatimah Samsurrijal,&nbsp;Ruzalina Baharin,&nbsp;Naurah Mat Isa,&nbsp;Muhammad Azrul Zabidi,&nbsp;Siti Noor Fazliah Mohd Noor","doi":"10.1002/jbm.a.37842","DOIUrl":"10.1002/jbm.a.37842","url":null,"abstract":"<div>\u0000 \u0000 <p>Gamma irradiation is an effective technique for biocomposite films intended for application in tissue engineering (TE) to ensure sterility and patient safety prior to clinical applications. This study proposed a biocomposite film composed of natural polymer chitosan (CS) and synthetic polymer poly-Ɛ-caprolactone (PCL) reinforced with sol–gel-derived bioactive glass (BG) for potential application in TE. The BG/PCL/CS biocomposite film was sterilized using 25 kGy gamma rays, and subsequent changes in its characteristics were analyzed through mechanical and physical assessment, bioactivity evaluation via immersion in simulated body fluid (SBF) and biocompatibility examination using human primary dermal fibroblasts (HPDFs). Results indicated a homogeneous distribution of BG particles within the BG/PCL/CS polymer matrix which enhanced bioactivity, and the polymer blend provide a structurally stable film. Gamma irradiation induced an increase in the film's surface roughness due to photo-oxidative degradation; however, this did not adversely affect the integrity of glass particles and polymer chains. In vitro assessments demonstrated hydroxyapatite formation on the film's surface, suggesting bioactivity. Biocompatibility testing confirmed enhanced cell adhesion and proliferation. These multifunctional properties highlight the potential of the fabricated BG/PCL/CS biocomposite film for TE and regenerative medicine applications.</p>\u0000 </div>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142820484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tuning Surface Chemistry Impacts on Cardiac Endothelial and Smooth Muscle Cell Development","authors":"Yasemin Acar,&nbsp;Amy Managh,&nbsp;Eric James Hill,&nbsp;Paul Roach","doi":"10.1002/jbm.a.37846","DOIUrl":"10.1002/jbm.a.37846","url":null,"abstract":"<p>Cardiovascular diseases (CVDs) are the leading causes of death worldwide, with approx. Twenty million deaths in 2021. Cardiovascular implants are among the most used biomaterials in the clinical world. However, poor endothelialisation and rapid thrombosis remains a challenge. Simple chemical surface modification techniques can be used to steer biological interactions without affecting the bioimplants' overall bulk characteristics such as radiopacity and flexibility. Although silanes are well studied for protein and cell interactions, the methodical investigation of cardiac endothelial cell (EC) alongside smooth muscle cell (SMC) to mimic natural arterial environments has been limited. In this study, these cells have been investigated on surfaces functionalized with methyl, amine, thiol, methacrylate, and fluorine organosilane groups. Cardiac EC and SMC growth was investigated with metabolic activity, time lapse imaging, and immunofluorescent staining techniques. The results demonstrated that the surfaces tested are able to selectively regulate the viability and growth of the cells. Aminosilane modified surfaces displayed 2-fold higher metabolic activity with HUVEC and 2-fold less metabolic activity with HCASMC cell lines, compared to tissue culture plastic controls. The amino-modification outperformed all other chemistries tested in terms of ability to promote the proliferation of ECs, while importantly reducing the activity of SMCs. This report demonstrates that aminosilane modified surfaces have the potential to be utilized in novel cardiovascular implants, which could improve biological integration in the short and possibly longer-term. The findings of this study suggest that specific chemical modifications of the surface can enhance endothelial cell activity while minimizing the proliferation of smooth muscle cells, which are often associated with thrombosis. This highlights the potential of carefully engineered surface chemistries to improve the clinical outcomes of cardiovascular implants.</p>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.a.37846","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142820491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Decellularized Extracellular Matrix and Polyurethane Vascular Grafts Have Positive Effects on the Inflammatory and Pro-Thrombotic State of Aged Endothelial Cells","authors":"Sophie J. Specht,&nbsp;Sabrina Rohringer,&nbsp;Pia Hager,&nbsp;Christian Grasl,&nbsp;Anna-Maria Schmitt,&nbsp;Virginia J. C. Pach,&nbsp;Katharina Ehrmann,&nbsp;Stefan Baudis,&nbsp;Robert Liska,&nbsp;Herbert Kiss,&nbsp;Karl H. Schneider,&nbsp;Bruno K. Podesser,&nbsp;Helga Bergmeister","doi":"10.1002/jbm.a.37830","DOIUrl":"10.1002/jbm.a.37830","url":null,"abstract":"<p>In vitro assessment of small-diameter synthetic vascular grafts usually uses standard cell culture conditions with early-passage cells. However, these conduits are mainly implanted in elderly patients and are subject to complex cellular interactions influenced by age and inflammation. Understanding these factors is central to the development of vascular grafts tailored to the specific needs of patients. In this study, the effects of aged endothelial cells subjected to pro- and anti-inflammatory agents and cultivated on a newly developed biodegradable electrospun thermoplastic polyurethane/poly(urethane-urea) blend (TPU/TPUU), on clinically available expanded polytetrafluorethylene (ePTFE), and on decellularized extracellular matrix (dECM) grafts were investigated. Young and aged endothelial cells were exposed to pro- and anti-inflammatory agents and characterized by morphology, migration capacity, and gene expression. In addition, the cells were seeded onto the various graft materials and examined microscopically alongside gene expression analyses. When exposed to pro-inflammatory cytokines, young and aged cells demonstrated signs of endothelial activation. Cells seeded on ePTFE showed reduced attachment and increased expression of pro-inflammatory genes compared with the other materials. dECM and TPU/TPUU substrates provided better support for endothelialization with aged cells under inflammatory conditions compared with ePTFE. Moreover, TPU/TPUU showed positive effects on reducing pro-thrombotic and pro-inflammatory gene expression in endothelial cells. Our results thus emphasize the importance of developing new synthetic graft materials as an alternative for clinically used ePTFE.</p>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.a.37830","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142751125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In Vitro Osteogenic Response to Copper-Doped Eggshell-Derived Hyroxyapatite With Macrophage Supplements","authors":"Tejal V. Patil,&nbsp;Dinesh K. Patel,&nbsp;Ki-Taek Lim","doi":"10.1002/jbm.a.37838","DOIUrl":"https://doi.org/10.1002/jbm.a.37838","url":null,"abstract":"<div>\u0000 \u0000 <p>The high bioactivity and biocompatibility of hydroxyapatite (HAP) make it a useful bone graft material for bone tissue engineering. However, the development superior osteoconductive and osteoinductive materials for bone regeneration remains a challenge. To overcome these constraints, Cu-doped hydroxyapatite (HAP(Cu)) from waste eggshells has been produced for bone tissue engineering. The materials produced were characterized using Fourier transform infrared spectroscopy, x-ray diffraction, and photoelectron spectroscopy. The scanning microscopy images revealed that the developed HAP was a rod-like crystalline structure with a typical 80–150 nm diameter. Energy-dispersive x-ray spectroscopy showed that the generated HAP was mostly composed of calcium, oxygen, and phosphorus. The Ca/P molar ratios in eggshell-derived and copper-doped HAP were 1.61 and 1.67, respectively, similar to the commercially available HAP ratio (1.67). The WST-8 assay was used to assess the biocompatibility of HAPs with hBMSCs. HAP(Cu) in the media significantly altered the cytotoxicity of biocompatible HAP(Cu). The osteogenic potential of HAP(Cu) was demonstrated by greater mineralization than that of pure HAP or the control. HAP(Cu) showed higher osteogenic gene expression than pure HAP and the control, indicating its stronger osteogenic potential. Furthermore, we assessed the effects of sample-treated macrophage-derived conditioned medium (CM) on hBMSCs' osteogenesis. CM-treated HAP(Cu) demonstrated a significantly higher osteogenic potential vis-à-vis pure HAP(Cu). These findings revealed that HAP(Cu) with CM significantly improved osteogenesis in hBMSCs and can be explored as a bone graft in bone tissue engineering.</p>\u0000 </div>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142749316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}