Influence of Seven Ion Species on Osteogenic Differentiation of Mesenchymal Stem Cells Stimulated by Macrophages in Indirect and Direct Coculture Systems

IF 3.9 3区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of biomedical materials research. Part A Pub Date : 2025-01-13 DOI:10.1002/jbm.a.37875

Akiko Obata, Makito Iguchi, Hikaru Yasue, Toshihiro Kasuga

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引用次数: 0

Abstract

Implanted biomaterials release inorganic ions that trigger inflammatory responses, which recruit immune cells whose biochemical signals affect bone tissue regeneration. In this study, we evaluated how mouse macrophages (RAW264, RAW) and mesenchymal stem cells (KUSA-A1, MSCs) respond to seven types of ions (silicon, calcium, magnesium, zinc, strontium, copper, and cobalt) that reportedly stimulate cells related to bone formation. The collagen synthesis, alkaline phosphatase activity, and osteocalcin production of the MSCs varied by ion dose and type after culture in the secretome of RAW cells. However, DNA production was relatively unaffected. The MSC secretome may also stimulate RAW cells in coculture and, therefore, affect osteogenic differentiation of MSCs. Overall, the ions often exerted different effects on each cell type. This study guides future work that explores the mechanisms behind ion-dependent osteogenic differentiation and cell functions.

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七种离子对间接和直接共培养系统中受巨噬细胞刺激的间充质干细胞成骨分化的影响

植入的生物材料释放无机离子，引发炎症反应，从而招募免疫细胞，其生化信号影响骨组织再生。在这项研究中，我们评估了小鼠巨噬细胞（RAW264， RAW）和间充质干细胞（kasa - a1, MSCs）对七种离子（硅、钙、镁、锌、锶、铜和钴）的反应，据报道，这些离子刺激与骨形成相关的细胞。RAW细胞分泌组培养后，MSCs的胶原合成、碱性磷酸酶活性和骨钙素产生随离子剂量和类型的不同而变化。然而，DNA的产生相对不受影响。间充质干细胞分泌组也可能在共培养中刺激RAW细胞，从而影响间充质干细胞的成骨分化。总的来说，离子通常对每种细胞类型产生不同的影响。这项研究指导了未来探索离子依赖性成骨分化和细胞功能背后机制的工作。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of biomedical materials research. Part A 工程技术-材料科学：生物材料

CiteScore

10.40

自引率

2.00%

发文量

135

审稿时长

3.6 months

期刊介绍： The Journal of Biomedical Materials Research Part A is an international, interdisciplinary, English-language publication of original contributions concerning studies of the preparation, performance, and evaluation of biomaterials; the chemical, physical, toxicological, and mechanical behavior of materials in physiological environments; and the response of blood and tissues to biomaterials. The Journal publishes peer-reviewed articles on all relevant biomaterial topics including the science and technology of alloys,polymers, ceramics, and reprocessed animal and human tissues in surgery,dentistry, artificial organs, and other medical devices. The Journal also publishes articles in interdisciplinary areas such as tissue engineering and controlled release technology where biomaterials play a significant role in the performance of the medical device. The Journal of Biomedical Materials Research is the official journal of the Society for Biomaterials (USA), the Japanese Society for Biomaterials, the Australasian Society for Biomaterials, and the Korean Society for Biomaterials. Articles are welcomed from all scientists. Membership in the Society for Biomaterials is not a prerequisite for submission.