Journal of biomedical materials research. Part A最新文献

{"title":"Coaxial Electrospinning of Multicomponent Fiber Membranes for Sustained Oxygen Supply in Hypoxic Environment","authors":"Brooke Campbell,&nbsp;Houra Mobaleghaleslam,&nbsp;Robert Horvath,&nbsp;Daewoo Han,&nbsp;Hiroyuki Kato,&nbsp;Hirotake Komatsu,&nbsp;Andrew J. Steckl","doi":"10.1002/jbm.a.70147","DOIUrl":"https://doi.org/10.1002/jbm.a.70147","url":null,"abstract":"<p>A major challenge for cell and tissue transplantation is providing a sustaining environment for newly transplanted cells and tissues. They require a temporary oxygen source since newly transplanted cells and tissues are not yet connected to the recipient's vascular system. Here we report on core-sheath fiber membranes formed using the coaxial electrospinning process that contain calcium peroxide (CPO) embedded in the fiber core to provide a source of oxygen by diffusion from the fibers over a period of multiple days. Embedding the enzyme catalase in the fiber sheath as a catalyst is found to significantly reduce the production of cytotoxic hydrogen peroxide (H₂O₂) by ~59% and to increase the overall amount of oxygen released up to ~28% after 4 days of release. CPO-containing PCL/PVP-PEO core-sheath fiber membranes produced enhanced cell viability of &gt; 90% at Day 5 for metabolically active pancreatic beta cell spheroids in a hypoxic environment, while non-CPO control cases resulted in no surviving cells at Day 5. Preliminary investigations with the incorporation of lysozyme as a model protein in the fiber core for eventual growth factor incorporation have yielded promising sustained release of lysozyme up to 10 days.</p>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"114 9","pages":""},"PeriodicalIF":3.8,"publicationDate":"2026-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.a.70147","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148878424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biomaterial-Activated Macrophage-T Cell Crosstalk Promotes Myofibroblastic Differentiation of Mesenchymal Stem Cells","authors":"Derek Avery,&nbsp;Luke Sheakley,&nbsp;Madison Gary,&nbsp;Sanjana Davis,&nbsp;Lais Morandini,&nbsp;Kelly M. Hotchkiss,&nbsp;Rene Olivares-Navarrete","doi":"10.1002/jbm.a.70146","DOIUrl":"https://doi.org/10.1002/jbm.a.70146","url":null,"abstract":"<div>\u0000 \u0000 <p>Biomaterials for orthopedic and dental implantation are designed to promote the timely resolution of inflammation and new bone formation while limiting the deposition of fibrotic tissue, which can cause implant failure. During the immune response to implantation, T cells at the peri-implant interface release cytokines that modulate innate immune cell response, mesenchymal stem cell (MSC) recruitment and proliferation, and new bone formation. However, T cells contribute to fibrosis in tissues such as the heart, lungs, liver, kidneys, and skin. This study aimed to determine how T cells contribute to peri-implant fibrosis using two biomaterials with differing inflammatory responses: pure titanium (Ti) and polyetheretherketone (PEEK). Following implantation of these materials in mice, we found greater recruitment of pro-inflammatory macrophages, CD4⁺ T cells, and CD8⁺ T cells in response to PEEK implants than to Ti implants. Likewise, macrophages cultured on PEEK surfaces demonstrated higher gene expression and protein secretion of pro-inflammatory factors (IL-6, IL-17A, TNF-α, and TGF-β1) than on Ti, an effect robustly enhanced when cells were cocultured with T cells. Finally, MSCs cultured with the conditioned media from macrophage-T cell co-cultures on PEEK had high expression of pro-fibrotic genes. Our findings demonstrate that biomaterial properties directly contribute to MSC differentiation toward myofibroblasts through the activation and crosstalk of macrophages and T cells, suggesting that these cells play a significant role in the fibrotic encapsulation of biomaterials.</p>\u0000 </div>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"114 9","pages":""},"PeriodicalIF":3.8,"publicationDate":"2026-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148878363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual Enzyme-Loading MnO2 Nanosystem for Cascade Chemodynamic Therapy With Enhanced Penetration in Superficial Tumors","authors":"Yingwei Tang,&nbsp;Di Li,&nbsp;Bo Zhang,&nbsp;Hongwei Duan","doi":"10.1002/jbm.a.70149","DOIUrl":"https://doi.org/10.1002/jbm.a.70149","url":null,"abstract":"<div>\u0000 \u0000 <p>The therapeutic effectiveness of superficial tumors is currently limited by the operational difficulty of traditional surgery. Localized target delivery of nanomedicine provides a promising solution for superior antitumor effect in superficial tumors, but its delivery efficiency can be greatly hampered by the dense tumor stroma. Compared with external modulation against the tumor extracellular matrix by physical methods, internal regulation of the tumor microenvironment via enzyme enables more direct degradation of the tumor extracellular matrix and robust antitumor efficacy. Herein, we report a dual-enzyme delivery nanoplatform to impart a cascade catalytic reaction for potent chemodynamic therapy with enhanced penetration depth in the superficial tumor. In response to the tumor microenvironment, the glucose oxidase/papain complex loaded in MnO₂ nanoparticles could be rapidly released to trigger cascade chemodynamic therapy and grant deeper penetration of therapeutic effect in the superficial tumor. The collective actions of papain's protease and peroxidase-like activity allowed significant tumor penetration and reactive oxygen species production cascaded with glucose oxidase, holding great promise as a potential strategy to improve the efficacy of superficial tumor treatment.</p>\u0000 </div>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"114 9","pages":""},"PeriodicalIF":3.8,"publicationDate":"2026-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148878307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Artificial Intelligence in Biomaterials: Revolutionizing Design and Manufacturing","authors":"Yichen Liu,&nbsp;Zhiting Wang,&nbsp;Haojie Wei,&nbsp;Xili Ding,&nbsp;Yubo Fan","doi":"10.1002/jbm.a.70144","DOIUrl":"https://doi.org/10.1002/jbm.a.70144","url":null,"abstract":"<div>\u0000 \u0000 <p>Biomaterials are indispensable to modern healthcare, yet their development remains hindered by traditional trial-and-error approaches that are costly, time-intensive, and inefficient. Artificial intelligence (AI) offers a powerful alternative, but most existing reviews treat AI merely as a computational tool for property prediction rather than as a systemic driver of innovation across the entire biomaterials' lifecycle. This review bridges that gap by providing a holistic, end-to-end perspective that integrates AI-driven design, intelligent manufacturing, and clinical translation into a unified framework. We systematically examine how AI-powered predictive modeling and structure–function optimization are reshaping the discovery of polymers, proteins, drug carriers, and tissue scaffolds, while also driving breakthroughs in 3D bioprinting, laser processing, composite manufacturing, and genetically programmed bio-fabrication. Recognizing that computational promise does not automatically translate into clinical reality, we critically evaluate persistent barriers such as data scarcity, poor data quality, the interpretability-accuracy trade-off, and regulatory uncertainties, and discuss strategies to overcome them through Explainable AI and rigorous validation protocols. Looking forward, we explore the convergence of AI with quantum computing and CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) gene editing, which together could enable a new generation of multifunctional, responsive biomaterials. By synthesizing advances across the full lifecycle and offering a structured framework for selecting AI methodologies tailored to specific biomaterials challenges, this review aims to serve as both a comprehensive resource and a strategic roadmap for translating computational innovation into tangible clinical impact.</p>\u0000 </div>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"114 9","pages":""},"PeriodicalIF":3.8,"publicationDate":"2026-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148878364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Graphene-Based Nanomaterials as Bioactive Agents and Drug Delivery Systems for Bone Tissue Engineering","authors":"Sijian Yu,&nbsp;Jianhong Liu,&nbsp;Xueer Zhou,&nbsp;Qiang Peng","doi":"10.1002/jbm.a.70129","DOIUrl":"10.1002/jbm.a.70129","url":null,"abstract":"<div>\u0000 \u0000 <p>Graphene-based nanomaterials (GBNs), such as graphene oxide and reduced graphene oxide, have been considered as promising functional materials for bone tissue engineering due largely to their intrinsic biological functions and great drug delivery capacity. As bioactive materials, GBNs possess antibacterial activity, photothermal effect, and the ability to regulate immune microenvironment and osteogenesis-related signaling pathways such as Wnt/β-catenin, MAPK, and BMP/Smad. As drug delivery systems, the large surface area and multiple functionalization sites allow GBNs to have great potential in loading and delivering various active agents, such as drugs, genes, and growth factors. Based on these properties, GBNs prevent infections, facilitate stem cell adhesion, proliferation, and osteogenic differentiation, and ultimately promote bone regeneration. In the past few years, reports regarding the applications of GBNs in bone tissue engineering have been well documented, but the clinical translation is still limited. Herein, on the basis of the two major roles of GBNs (bioactive agents and drug delivery systems), we aim to provide a comprehensive understanding of the multiple functions and mechanisms of GBNs in promoting bone tissue regeneration. The progress and challenges in clinical translation are also discussed.</p>\u0000 </div>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"114 9","pages":""},"PeriodicalIF":3.8,"publicationDate":"2026-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148893167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photo-Activated microRNA 20a Delivery via Core-Shell-Shell Nanoparticles for the Suppression of Head and Neck Cancer","authors":"Angelica M. Helton,&nbsp;Tyus J. Yeingst,&nbsp;Lindsay P. Stone,&nbsp;Dylan P. Kearse,&nbsp;Neerav Goyal,&nbsp;Douglas B. Stairs,&nbsp;Daniel J. Hayes","doi":"10.1002/jbm.a.70142","DOIUrl":"https://doi.org/10.1002/jbm.a.70142","url":null,"abstract":"<p>Current treatment modalities for head and neck cancer often leave patients with severe morbidities including difficulty swallowing, breathing, and speaking. Novel therapeutics are needed which induce suppress tumors, provide more precise surgical resection, and minimize damage to the complex surrounding tissue. In this study, plasmonic gold-silver-gold core-shell-shell (CSS) nanoparticles were functionalized with miR-20a-5p via a thermo-responsive furan-based Diels–Alder linker. The delivery of microRNA (miRNA) mimics allows for the post-transcriptional regulation of gene expression across numerous signaling pathways simultaneously. The CSS morphology and composition of the nanoparticles resulted in plasmonic maximum at 849 nm, within the near-infrared range. Upon photo-activation with an 850 nm LED, the linker underwent a retrograde Diels–Alder reaction releasing the miRNA mimic. In vitro results showed that 50 nM miR-20a-5p delivered with the CSS nanoparticles and photo-activated led to a 60% reduction in cancer cell survival. A combination of bioinformatic and gene expression analysis revealed that multiple gene targets of miR-20a-5p within the apoptosis signaling pathway were knocked down. Murine xenograft models emphasized spatiotemporal control of therapeutic release through photo-activation at 850 nm. Furthermore, FaDu cell tumor volume decreased ~95% within 48 h of a singular dose of 100 picomoles of miR-20a-5p from the CSS nanoparticles. Both in vitro and in vivo results exhibit the promise of the CSS nanoparticle system for miRNA mimic delivery. With responsivity in the near-infrared range, head and neck cancer applications are ideal for this system.</p>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"114 9","pages":""},"PeriodicalIF":3.8,"publicationDate":"2026-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.a.70142","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148878425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemo-Magnetic Targeted Dual-Drug-Loaded Nano Polymer-Conjugate for Combined Tumor and Thrombosis Therapy","authors":"Shenglong Ding,&nbsp;Mengjie Huang,&nbsp;Mingzhu Liu","doi":"10.1002/jbm.a.70151","DOIUrl":"https://doi.org/10.1002/jbm.a.70151","url":null,"abstract":"<div>\u0000 \u0000 <p>Most cancer patients are at high risk of thrombotic complications. However, tumor therapy combined with thrombosis treatment is a challenge due to the discrepancy in the microenvironment between tumors and thrombosis. In this study, a nano polymer-conjugate with tumor targeting and thrombolysis was developed. The nano polymer-conjugate is formed by a cross-linking agent containing imine bonds to cross-link anti-thrombosis drug nattokinase (NK) with a second-generation dendrimer (G₂) in the outer layer (NK-G₂), and magnetic silica is introduced during the cross-linking. Then, a dual drug loading system (NK-G₂)_<i>n</i>/FA-Dox is obtained by loading anti-tumor drug doxorubicin (Dox) modified with folic acid (FA). FA and the magnetic silica enable the nano polymer-conjugate to accumulate at the tumor sites. Most importantly, the nano polymer-conjugate shows weak acid responsiveness, allowing it to dissociate in the tumor microenvironment and release two drugs without entering cancer cells. This addresses the problem of the discrepancy in the microenvironment between tumors and thrombosis. This dual-drug loaded nano polymer-conjugate exhibits excellent potential in the combined treatment of tumor and the thrombotic complications.</p>\u0000 </div>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"114 9","pages":""},"PeriodicalIF":3.8,"publicationDate":"2026-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148878306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic Wound Healing Enhancement by Extracellular Vesicles From Engineered Macrophages and Fibroblasts","authors":"Ping Liang,&nbsp;Jialin Ye,&nbsp;Kaiyang Lin,&nbsp;Qinghua Luo,&nbsp;Haisu Zheng,&nbsp;Yuyao Hu,&nbsp;MingLang Zou,&nbsp;Yanting Huang,&nbsp;Sifang Wu,&nbsp;Qi Sun,&nbsp;Yansong Guo,&nbsp;Zuquan Weng","doi":"10.1002/jbm.a.70126","DOIUrl":"https://doi.org/10.1002/jbm.a.70126","url":null,"abstract":"<div>\u0000 \u0000 <p>Severe wound healing impairment risks persistent tissue damage and even necrosis. Cell-free exosome therapy demonstrates significant potential in wound healing due to its high efficacy and micro-volume characteristics. However, its clinical-scale translation faces challenges including low yield, limited sources, functional monotony, and low bioavailability. We developed a lentiviral co-engineering strategy introducing exosome secretion-related genes into macrophage and fibroblast cell lines, achieving stable, high-yield (twofold increase) exosome production. Co-engineered extracellular vesicles (EVs) demonstrated enhanced cellular uptake and synergistically upregulated pro-angiogenic (<i>HIF-1α</i>, <i>VEGF-A</i>) and anti-inflammatory (<i>IL-4</i>, <i>IL-10</i>) factors. In a cell model and murine full-thickness wound model, the combination of EVs from both sources potently accelerated healing, promoting re-epithelialization and collagen deposition while orchestrating an anti-inflammatory response via <i>TNF-α</i>/<i>IL-1β</i> downregulation and <i>IL-10</i> upregulation. In summary, co-cultured EVs not only exhibit high yield and purity but also demonstrate potent pro-angiogenic, anti-inflammatory, and antioxidant effects, effectively promoting wound healing, epithelial tissue regeneration, and collagen deposition. This study proposes an innovative and highly efficient method for synergistically regulating the production of EVs from cellular systems, providing promising research resources for their application in wound healing and medical esthetics. It also offers crucial insights for investigating the underlying mechanisms.</p>\u0000 </div>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"114 9","pages":""},"PeriodicalIF":3.8,"publicationDate":"2026-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148878365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mucus-Penetrating Nanostructured Lipid Carriers: An Effective Approach for Cannabidiol to Pass Across the Nasal Mucus-Mucosal Barrier","authors":"Liyan Wang,&nbsp;Bo Liu,&nbsp;Qing Luo,&nbsp;Kaili Liang,&nbsp;Wei Wang,&nbsp;Li Yang,&nbsp;Qing Wang","doi":"10.1002/jbm.a.70133","DOIUrl":"10.1002/jbm.a.70133","url":null,"abstract":"<div>\u0000 \u0000 <p>Effective nose-to-brain delivery is limited by the mucus-mucosal barrier, which severely hinders drug transport after nasal administration. Although nanostructured lipid carriers (NLCs) offer promising solutions by improving their retention duration in the mucosal layer or promoting mucosal permeation and intracellular uptake, the nanoparticle-mucus interactions, epithelial uptake, and mechanisms supporting transcellular transport in the nasal cavity remain poorly learned. This study was developed to assess the interactions between mucin and NLCs, the absorption and transport capabilities of Cannabidiol (CBD) nanostructured lipid carriers (CBD-NLCs) in rat nasal mucosal epithelial (RNME) cells. Molecular docking (MD) was used to evaluate the interactions between the human mucin protein MUC5AC (hMUC5AC) and some main components of CBD-NLCs. In vitro experiments were conducted to assess the mucus penetration of CBD-NLCs. Cellular uptake, localization, and transport mechanisms of coumarin-6-labeled NLCs (C6-NLCs) in RNME cells were examined by confocal laser scanning microscopy, endocytosis inhibition assays, and transcellular transport assays. Our results show that there were no interactions between the main components of NLCs and hMUC5AC according to MD simulations. In vitro experiments, NLCs promoted mucus penetration and had little interaction with mucin. Cellular studies confirmed cytoplasmic (non-nuclear) localization of C6-NLCs in RNME cells, and transport assays showed that the clathrin-mediated endocytic route was a predominant pathway for their internalization. When across the RNME cell monolayer, NLCs had a great benefit on CBD transportation across monolayers of RNME cells. Our results clarify the mechanism by which NLCs enhance mucosal penetration and facilitate cellular trafficking in RNME cells. Their little interaction with mucin, combined with efficient cellular uptake and transcellular transport, supports the potential of NLCs as a promising nanoplatform for nose-to-brain drug delivery.</p>\u0000 </div>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"114 9","pages":""},"PeriodicalIF":3.8,"publicationDate":"2026-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148893150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Kidney-Targeted Biomimetic Nanoparticles Loaded With Apolipoprotein H Alleviate Sepsis-Associated Acute Kidney Injury by Inhibiting the TLR4/NF-κB Pathway","authors":"Lijun Zhu,&nbsp;Ruyi He,&nbsp;Jincong Xiao,&nbsp;Guoqiang Hu,&nbsp;Kuangzhang You,&nbsp;Suhuan Rao","doi":"10.1002/jbm.a.70143","DOIUrl":"10.1002/jbm.a.70143","url":null,"abstract":"<div>\u0000 \u0000 <p>Sepsis-associated acute kidney injury (SA-AKI) is a common and life-threatening complication of sepsis, whose high incidence and mortality severely impair patient prognosis. Existing anti-AKI drugs exhibit limited efficacy due to their inability to efficiently target injured kidneys. Although kidney-targeted nanocarrier delivery systems show potential, exogenous nanoparticles are rapidly cleared by the immune system, resulting in a short blood circulatory half-life and unsatisfactory targeting efficiency. Recombinant apolipoprotein H (rAPOH) exhibits anti-apoptotic and anti-inflammatory activities, holding promise for SA-AKI treatment; however, its efficacy is constrained by insufficient targeting capability and rapid in vivo metabolic clearance. In this study, we constructed a biomimetic delivery system (KMA@rAPOH) consisting of A@rAPOH nanoparticles coated with kidney-targeting peptide-functionalized renal tubular epithelial cell membranes to realize targeted rAPOH delivery for SA-AKI therapy. The nanocarrier demonstrated satisfactory encapsulation efficiency, high drug loading capacity, and favorable controlled release, along with excellent long-term stability in DMEM and deionized water. In vitro, KMA@rAPOH was efficiently internalized by renal tubular epithelial cells and exerted significant anti-apoptotic and anti-inflammatory effects. In a mouse model of SA-AKI, KMA@rAPOH markedly alleviated renal pathological damage and inhibited cell apoptosis. Additionally, it reduced the proportion of CD11c⁺F4/80⁺ pro-inflammatory macrophages in the renal CD45⁺CD11b⁺ population. Mechanistically, KMA@rAPOH was found to reduce TLR4 levels and inhibit NF-κB p65 phosphorylation, accompanied by decreased secretion of pro-inflammatory cytokines including TNF-α, IL-1β, and IL-6. These observations indicate that the TLR4/NF-κB cascade may partially mediate the renoprotective activity of KMA@rAPOH, and this biomimetic nanoplatform represents a promising targeted delivery strategy for the treatment of renal inflammatory disorders.</p>\u0000 </div>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"114 8","pages":""},"PeriodicalIF":3.8,"publicationDate":"2026-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148709238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}