Journal of biomedical materials research. Part A最新文献

{"title":"Influence of Diol Chain Length on Various Properties of Citric Acid Polyesters/PLA Electrospun Nonwovens for Tissue Engineering Applications","authors":"Aleksandra Bandzerewicz,&nbsp;Adrian Chlanda,&nbsp;Tomasz Gołofit,&nbsp;Miroslav Slouf,&nbsp;Piotr Denis,&nbsp;Agnieszka Gadomska-Gajadhur","doi":"10.1002/jbm.a.37967","DOIUrl":"https://doi.org/10.1002/jbm.a.37967","url":null,"abstract":"<div>\u0000 \u0000 <p>Despite the great potential of citrate polyesters in regenerative medicine, the data about their application in electrospinning is somewhat limited. In this work, poly(dimethylene citrate) (P-1,2-ECit), poly(tetramethylene citrate) (P-1,4-BCit), and poly(hexamethylene citrate) (P-1,6-HCit) were synthesized. Nonwovens from poly(diol citrates)/PLA mixtures were successfully electrospun and characterized using SEM, AFM, water contact angle measurement, DSC, TGA, and in vitro degradation tests. The addition of poly(diol citrates) increases the hydrophilicity and surface adhesion force of PLA nonwovens; however, the observed effects depend on the scale level (macro/micro) of the analysis. Diol chain length in poly(diol citrate) influences the compatibility and heterogeneity of its distribution within the carrier polymer. Additionally, it impacts the crystallinity of the PLA phase. Degradation tests show the problem of the nonwoven stability in the aqueous media and the high leachability of the short-chained poly(diol citrates). Addressing this issue is important regarding controlling the degradation kinetics. Despite the good processability in electrospinning and promising surface properties of the poly(diol citrates)/PLA mixtures, the instability of these materials in an aqueous environment is an important issue which can subsequently affect the performance of the eventual implant/cell scaffold. The solution may involve chain elongation of the hydrophilic oligomeric additive.</p>\u0000 </div>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 8","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144681598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chondroprotective Effects of Chitosan-Coated Poly(Lactic-co-Glycolic Acid) Nanocapsulized Curcumin on Human Articular Chondrocytes","authors":"Yung-Hsin Cheng,&nbsp;Chang-Chin Wu,&nbsp;Yueh-Hsin Chen,&nbsp;Pei-Feng Huang,&nbsp;Che-Wei Hsu,&nbsp;Koichi Kato,&nbsp;Kai-Chiang Yang","doi":"10.1002/jbm.a.37969","DOIUrl":"https://doi.org/10.1002/jbm.a.37969","url":null,"abstract":"<div>\u0000 \u0000 <p>Dysregulation of pro-inflammatory cytokines participates in the initiation and development of knee osteoarthritis (OA). Consequently, interventions to boost the anti-inflammatory capacity of articular chondrocytes have been proposed to treat early-stage OA and prevent OA progression. Applying nanoencapsulation can enhance bioavailability and bioactivity and sustain the anti-inflammatory activity of phytochemicals. Accordingly, in this study, we used nanocapsules to deliver curcumin (Cur) to treat inflammatory chondrocytes. Using double-emulsion technology, Cur was encapsulated in chitosan-coated poly(lactic-co-glycolic acid) nanocapsules. The nanocapsulized Cur (NCcur) was characterized, and the toxicity to human articular chondrocytes was evaluated. NCcur was applied to interleukin-1 beta (IL-1β)-stimulated cells based on findings of the Cur toxicity study. Results showed that the particle size of NCcur was 247.8 ± 1.73 nm with a zeta potential of 20.3 ± 0.11 mV and a mid-range distribution. NCcur showed a core-shell and sphere-like morphology. The encapsulation efficiency of Cur in nanocapsules was 67.1%. Nanoencapsulation decreased the toxicity of high-dose Cur (&gt; 20 μM), and NCcur exhibited a sustained Cur release over 72 h. NCcur supplementation (20 μM) improved cell survival and ameliorated cell senescence of inflammatory chondrocytes. The IL-1β-induced <i>IL1B</i>, <i>IL6</i>, metalloproteinase-9 (<i>MMP9</i>), and <i>MMP13</i> mRNA expressions were down-regulated, while <i>IL10</i> level was enhanced in NCcur-treated chondrocytes. Likewise, NCcur supplementation restored aggrecan, collagen type II alpha 1 chain, and <i>SOX9</i> mRNA expressions. MMP-13, IL-8, and MCP-1 secretions in the supernatant also decreased. By applying nanocapsules, we assumed the anti-inflammatory capacity of Cur could be sustained for treating knee OA.</p>\u0000 </div>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 8","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144666614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Brillouin Spectroscopy: A Non-Invasive Method for Assessing the Viscoelastic Properties of Biologically Relevant Polymers","authors":"Vsevolod Cheburkanov,&nbsp;Sujeong Jung,&nbsp;Mykyta Kizilov,&nbsp;Samantha E. Holt,&nbsp;Daniel L. Alge,&nbsp;Taylor H. Ware,&nbsp;Vladislav V. Yakovlev","doi":"10.1002/jbm.a.37965","DOIUrl":"https://doi.org/10.1002/jbm.a.37965","url":null,"abstract":"<p>Research of biocompatible polymers is critical for advancing biomedical technologies, particularly in the fields of tissue engineering, drug delivery, and cardiovascular health. This study investigates the mechanical properties of a series of novel biocompatible polymers using Brillouin microspectroscopy. We demonstrate the ability of Brillouin spectroscopy to accurately measure mechanical properties of these polymers on a microscopic level, which are vital for their application and can be finely tuned to match the requirements. Our findings suggest that Brillouin microspectroscopy, coupled with Raman spectroscopy, offers a powerful complementary approach to traditional viscoelasticity measurement techniques, paving the way for enhanced characterization and utilization of biocompatible polymers in medical research and clinical practice. This in turn would help streamline production and control of these polymers in a non-invasive and label-free way.</p>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 7","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.a.37965","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144615395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Successful Preparation of Contrast Particle-Loaded Human Mesenchymal Stem Cell Aggregates Using Adherent Cell Self-Aggregation Technique","authors":"Lupeng Teng,&nbsp;Soichiro Fukushima,&nbsp;Makoto Koizumi,&nbsp;Hirotaka James Okano,&nbsp;Takao Ohki,&nbsp;Koji Matsuura,&nbsp;Ryosuke Iwai","doi":"10.1002/jbm.a.37964","DOIUrl":"https://doi.org/10.1002/jbm.a.37964","url":null,"abstract":"<div>\u0000 \u0000 <p>Several studies have investigated the location of transplanted cells and tissue-engineered cell constructs in the body by incorporating contrast nanoparticles into cells by endocytosis; however, these have yet to be applied clinically because of the complexity of assessing the safety of nanoparticles. In this study, we proposed that our developed adherent cell self-aggregation technique (CAT) could be used to develop cell aggregates loaded with contrast particles of a size that would exclude the possibility of endocytosis, and aimed to prepare these aggregates followed by biological and computed tomography (CT) contrast evaluation under X-rays. Once human bone marrow-derived mesenchymal stem cells (HBMSCs) were seeded into culture dishes coated with CAT-inducing polymer to form gapless cell monolayer sheets, tungsten carbide (WC) particles smaller than 1 μm or titanium (Ti) particles larger than 10 μm were added, and thus each particle deposited on the surface of the cell monolayer sheet. During the subsequent overnight incubation, spontaneous detachment and aggregation of the cell monolayer sheets with deposited WC and Ti particles occurred, forming single spherical cell aggregates (spheroids) and loading these particles. Histological analysis confirmed that Ti particles with a diameter of at least 10 μm were not endocytosed and remained attached to the outside of cells forming spheroids, while WC particles were endocytosed into the cells. The CT images of the Ti-loaded spheroids were clearly visible along the spheroid shape under X-ray irradiation. Then, we confirmed that there was no toxicity to the cells forming the spheroids by loading Ti particles, and the cells could sprout and proliferate by culturing the spheroids. We successfully prepared Ti particle-loaded HBMSCs aggregates with long fiber shape (&gt; 10 cm) by applying CAT to a culture dish with a ring-fiber-shaped culture groove and confirmed their clear visibility on CT images under X-ray irradiation, as well as their containment and ejection into a catheter, demonstrating their applicability to catheter-mediated regenerative therapy.</p>\u0000 </div>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 7","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144598404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing Bone Regeneration: The Role of Biomimetic Silicified Collagen Scaffold in Osteogenesis and Angiogenesis","authors":"Ming-yuan Liu,&nbsp;Yu-xuan Ma,&nbsp;Lei Chen,&nbsp;Meng Wang,&nbsp;Zheng-long Zhang,&nbsp;Yu-xia Hou,&nbsp;Li-na Niu","doi":"10.1002/jbm.a.37954","DOIUrl":"https://doi.org/10.1002/jbm.a.37954","url":null,"abstract":"<div>\u0000 \u0000 <p>The identification of materials that effectively promote mineralization and vascularization is crucial for advancing clinical applications in bone regeneration. Biomimetic silicified collagen scaffold (SCS) has emerged as a promising candidate, demonstrating significant potential to enhance both osteogenesis and angiogenesis. However, the mechanisms by which SCS directly influences angiogenesis to facilitate bone defect healing remain largely unexplored. In this study, we observed that the implantation of SCS in rabbit femoral defects resulted in extensive bone regeneration and angiogenesis at the wound sites. Notably, SCS outperformed commercial alternatives such as Bio-Oss in terms of degradation and angiogenic response. In vitro assays further demonstrated that SCS upregulates angiogenic protein expression and promotes endothelial cell angiogenesis through the activation of the HIF-1α/VEGF signaling pathway. Consequently, SCS modulates the phenotype of vascular endothelial cells, leading to the formation of CD31^hiEmcn^hi type H endothelial cells, which are critical for effective bone regeneration. This study offers valuable perspectives on the dual effects of silicified materials on osteogenesis and angiogenesis, advancing the understanding of their potential functions in regenerative medicine.</p>\u0000 </div>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 7","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144589633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exponentially Decreasing Antigen Release Reduces Inflammatory Markers in an Antigen-Specific Manner","authors":"Arezoo Esrafili,&nbsp;Aleksandr Talitckii,&nbsp;Joshua Kupfer,&nbsp;Abhirami Thumsi,&nbsp;Madhan Mohan Chandra Sekhar Jaggarapu,&nbsp;Margaret Dugoni,&nbsp;Gregory Jensen,&nbsp;Matthew M. Peet,&nbsp;Julianne L. Holloway,&nbsp;Abhinav P. Acharya","doi":"10.1002/jbm.a.37962","DOIUrl":"https://doi.org/10.1002/jbm.a.37962","url":null,"abstract":"<p>Vaccine development requires innovative approaches to improve immune responses while reducing the number of immunizations. In this study, we explore the impact of controlled antigen release on immune activation and regulation using programmable infusion pumps and biodegradable biomaterials in OT-II and wild-type mice to understand the adaptive immune response through controlled antigen delivery in the absence of adjuvant. Ovalbumin (OVA) was delivered via an exponentially decreasing profile, mimicking clearance of infection, and an exponentially increasing profile, mimicking induction of infection. Exponentially decreasing OVA delivery through infusion pumps promoted regulatory T-cell (Treg) activation in secondary lymphoid organs and suppressed pro-inflammatory T-helper type 17 (Th17) responses in blood. An exponentially increasing OVA profile enhanced central memory T-cell (TCM) populations in submandibular blood and humoral immune responses in cardiac blood serum, demonstrating distinct immune modulation based on release kinetics. OVA was also delivered using a biodegradable PLGA-PEG-PLGA (PPP) depot, which provided controlled OVA release in an exponentially decreasing pattern. PPP-OVA treatment significantly reduced the frequency of pro-inflammatory T-helper type 1 (Th1) cells while increasing CD25⁺FOXP3⁺ Treg cells in the spleen. Moreover, to identify T-cell populations that most accurately characterize the divergence in Treg and T-helper response to OVA kinetics, a Sequential Feature Selection (SFS) algorithm with Machine Learning (ML) models was used. ML algorithms identified gMFI of RORγt⁺ as a key feature in submandibular blood and the ratio of gMFI of FOXP3⁺ to GATA3⁺ as the marker that was significantly changed by treatments in inguinal lymph nodes (iLN) when infusion pumps were used to deliver OVA. In addition, ML-based SFS identified CD25⁺FOXP3⁺ regulatory T cells as the most important feature, influencing the expression of other cell types in both inguinal lymph nodes (iLN) and spleen when PPP was used to deliver OVA. This finding suggests that the exponentially decreasing profile may generate anti-inflammatory responses. Overall, these findings suggest that controlled antigen delivery enhances immune regulation and memory T cells, providing new insights into immune responses mediated by the release kinetics.</p>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 7","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.a.37962","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144582064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biodegradable, Antibacterial TCP Implant Coatings With Magnesium Phosphate-Based Supraparticles","authors":"Maria Carolina Lanzino,&nbsp;Anika Höppel,&nbsp;Long-Quan R. V. Le,&nbsp;Stefania Morelli,&nbsp;Andreas Killinger,&nbsp;Wolfgang Rheinheimer,&nbsp;Hermann O. Mayr,&nbsp;Sofia Dembski,&nbsp;Ali Al-Ahmad,&nbsp;Moritz F. Mayr,&nbsp;Uwe Gbureck,&nbsp;Michael Seidenstuecker","doi":"10.1002/jbm.a.37963","DOIUrl":"https://doi.org/10.1002/jbm.a.37963","url":null,"abstract":"<p>This work highlights the potential of porous, bioactive coatings to advance implant technology and address critical clinical challenges. A key issue in implant coatings is to achieve the balance between infection prevention and successful osseointegration. Although titanium implants are widely used due to their mechanical strength and biocompatibility, their bioinert nature limits integration with bone tissue. To address these issues, porous calcium phosphate (CaP) coatings have been developed to enhance cell attachment and bone growth. However, CaP, especially in the widely used form of hydroxyapatite (HAp), has a low resorption rate, which often leads to prolonged coating stability and impairs natural bone remodeling. To overcome this limitation, magnesium phosphate (MgP), an underexplored but promising biomaterial with high biocompatibility and osteogenic potential, can be introduced. Another innovative strategy is the doping of biomaterials with antibacterial ions, among which copper (Cu) has attracted particular attention. The incorporation of Cu into the coating matrix can significantly reduce the risk of post-operative infection while promoting angiogenesis, a key factor for rapid and stable implant integration. This study presents bone implant coatings composed of tricalcium phosphate (TCP) and Cu-doped MgP clustered nanoparticles (supraparticles) fabricated via high-velocity suspension flame spraying (HVSFS). This particle system addresses current challenges in bone tissue regeneration by synergistically combining the high biodegradability of MgP, the bone-mimicking properties of CaP, and the antibacterial capabilities of Cu. In addition, the HVSFS process enables the creation of thin layers with porous microstructures. Biocompatibility of the prepared coatings was assessed using MG63 osteosarcoma cells, while the antibacterial efficacy was tested against <i>Staphylococcus aureus</i> and <i>Escherichia coli</i>. The incorporation of Cu-doped MgP supraparticles (MgPCu and MgPCu HT) into TCP coatings resulted in high Cu release and pronounced antibacterial efficacy compared to the TCP reference, while the addition of Cu-doped FT supraparticles (FTCu) led to high cell proliferation.</p>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 7","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.a.37963","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144573153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Design of Experiment to Evaluate the Printability for Bioprinting by Using Deep Learning Image Similarity","authors":"Leon Balters,&nbsp;Stephan Reichl","doi":"10.1002/jbm.a.37961","DOIUrl":"https://doi.org/10.1002/jbm.a.37961","url":null,"abstract":"<p>Bioprinting is a growing area in the field of tissue engineering that offers a potential solution to the global shortage of organ transplants. Ensuring high printability is crucial for bioprinting. To better understand printability, a design of experiment model that examines printing speed and pressure in extrusion-based printing was developed. Two biomaterials, hyaluronic acid and sodium alginate, were selected as surrogate biomaterials to understand how rheological properties play a role in printability. Various rheological aspects such as shear-thinning behavior, viscosity, and recovery were investigated. To further evaluate printability, a new method was used that includes deep learning image similarity. The information obtained with the surrogate bioinks was then applied to another biomaterial, methacrylated hyaluronic acid, in combination with corneal keratocytes to demonstrate the successful implementation of the outcome of this design of experiment. As a result of this study, a better understanding of the rheological properties for bioprinting was achieved, leading to a next step towards improving extrusion-based bioprinting, which can be used for a wide range of applications.</p>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 7","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.a.37961","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144558004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Additive Manufacturing and Sterilization on Poly(p-Dioxanone) for Short-Term Application in the Intestinal Environment","authors":"Stefanie Ficht,&nbsp;Lukas Schübel,&nbsp;Diana M. Rojas-González,&nbsp;Juliana Dos Santos Solheid,&nbsp;Stefan Leonhardt,&nbsp;Magdalena Kleybolte,&nbsp;Cécile Boudot,&nbsp;Markus Eblenkamp,&nbsp;Jana Steger,&nbsp;Dirk Wilhelm,&nbsp;Petra Mela","doi":"10.1002/jbm.a.37957","DOIUrl":"https://doi.org/10.1002/jbm.a.37957","url":null,"abstract":"<p>Additive manufacturing of patient specific implants made of biodegradable polymers is receiving increasing attention in the medical sector, including the trend towards manufacturing at the point-of-care. Despite this, the changes of the polymer structure and their effects on mechanical properties and degradation behavior caused by the additive manufacturing process and subsequent sterilization are still insufficiently investigated, although of key relevance for the implant's functionality. In this study, poly(p-dioxanone) (PPDO) was processed by fused filament fabrication (FFF). The effects of manufacturing as well as two different low-temperature sterilization techniques, namely H₂O₂ plasma and gamma irradiation, on the polymer structure were evaluated. Additionally, PPDO degradation was investigated by immersing the processed samples in Sorensen's phosphate buffer (PB) with pH = 6.47 for 28 days to mimic implantation in intestinal milieu and evaluated at regular time intervals. Results showed that we were able to successfully print PPDO without influencing the polymer structure or cytocompatibility. No significant changes were detected for plasma-sterilized samples (PS) while gamma-sterilized (GS) ones significantly decreased molecular weight (M_w and M_n) and showed significant lower inherent viscosity (IV) compared with the (non-sterilized) control group after processing. During immersion in PB, a decrease in M_w, M_n, and mechanical strength occurred for all samples. However, GS samples were affected to a much higher extent compared with the other groups both in final values and timeline. A degradation plateau was seen for the tensile strength of NS and PS samples over the first 21 and 17 days, respectively, followed by a steady decrease. In contrast, for the GS samples, a drastic decrease in tensile strength occurred already during the first 14 days. There was no notable mass loss detected within the first 28 days of degradation for any of the sample groups. Based on these results, we conclude that FFF with subsequent plasma sterilization is a reliable process for manufacturing PPDO devices for short-term applications that require stable mechanical conditions within the first weeks of implantation to guarantee the time needed for tissue healing before degrading, as for example, in the case of intestinal compression anastomoses. Such requirement could not be met with gamma sterilization with the dose used, because of the too fast decrease in mechanical properties.</p>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 7","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.a.37957","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144558107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and Evaluation of an Innovative S-Flurbiprofen-Diethylamine Emulgel With Superior Transdermal Delivery and Analgesic Activity","authors":"Jian Zhang,&nbsp;Jinmeng Han,&nbsp;Anqi Zhu,&nbsp;Xiaoqian Zhu,&nbsp;Yuyu Zeng,&nbsp;Mingyue Yin,&nbsp;Yang Xiao,&nbsp;Chenchen Wang,&nbsp;Chaoyang Yin,&nbsp;Xingyin Wang,&nbsp;Guisen Zhang,&nbsp;Chao Hao","doi":"10.1002/jbm.a.37936","DOIUrl":"https://doi.org/10.1002/jbm.a.37936","url":null,"abstract":"<div>\u0000 \u0000 <p>This study aimed to develop an innovative (S-flurbiprofen)-diethylamine (SFP-DEA) emulgel formulation via incorporating SFP as the active pharmaceutical ingredient within a carbomer 940 gel matrix. SFP-DEA emulgel was synthesized by dissolving SFP-DEA in the aqueous phase of an oil-in-water (O/W) emulsion, followed by dispersion into a carbomer 940 gel matrix. The physicochemical stability of SFP-DEA emulgel was evaluated via centrifuge, temperature swing test, high temperature, and long-term storage at ambient conditions. Ex vivo SFP transdermal delivery of SFP-DEA emulgel was evaluated using a Franz diffusion cell combined with excised rat skin. The in vivo analgesic activity and skin irritation test of SFP-DEA emulgel were evaluated using a mouse knee osteoarthritis model and healthy rats, respectively. Results demonstrated that SFP-DEA emulgel showed robust physicochemical stability and retain a final SFP content of 1.5% (w/w). Ex vivo transdermal study demonstrated that EMG5 (the emulgel optimized with laurocapram and menthol as penetration enhancers) achieved an 8-h cumulative SFP transdermal flux of 741.28 μg/cm² (44.23% of the administered dose), which is 27.94-fold higher than that of Loqoa (SFP tapes). In addition, SFP-DEA emulgel demonstrated rapid analgesic efficacy, with an 84.36% pain inhibition rate within 30 min in the osteoarthritis model, and elicited no signs of skin irritation in rats. In conclusion, the SFP-DEA emulgel developed herein exhibits high stability, enhanced transdermal delivery, preliminary analgesic activity, and favorable safety profiles, positioning it as a promising topical therapeutic candidate.</p>\u0000 </div>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 7","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144558290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}