Journal of biomedical materials research. Part A最新文献

{"title":"Effect of Osteoblast-Extracellular Matrix-Functionalized Nanostructured Titanium Surface on Osteoblastic Cell Behavior","authors":"Georgia Kors Quiles,&nbsp;Paola Gomes Souza,&nbsp;Maria Paula Oliveira Gomes,&nbsp;Rayana Longo Bighetti-Trevisan,&nbsp;Leticia Faustino Adolpho,&nbsp;Helena Bacha Lopes,&nbsp;Adalberto Luiz Rosa,&nbsp;Marcio Mateus Beloti","doi":"10.1002/jbm.a.70131","DOIUrl":"https://doi.org/10.1002/jbm.a.70131","url":null,"abstract":"<p>Nanostructured titanium (Ti) facilitates osteoblast differentiation by modulating various signaling pathways and may enhance osseointegration, potentially through its influence on the formation and remodeling of the extracellular matrix (ECM). This study aimed to investigate the impact of osteoblast-ECM-functionalized nanostructured Ti surface on osteoblastic cell behavior. Initially, it was demonstrated that nanotopography upregulated the expression of genes associated with ECM formation and remodeling in MC3T3-E1 osteoblastic cells compared to a polished Ti surface. Then, MC3T3-E1 cells were cultured on nanotopography, and the cultures were decellularized to generate ECM-functionalized nanostructured Ti surface. Successful decellularization was verified by 4′,6-diamidino-2-phenylindole staining for DNA, DNA quantification, fibronectin immunodetection and scanning electron microscopy. To investigate the effect of ECM functionalization on osteoblasts, calvaria-derived osteoblasts were cultured on both non-functionalized and osteoblast-ECM-functionalized nanostructured Ti surfaces. ECM functionalization did not affect cell morphology but reduced proliferation and the gene expression of osteoblastic markers while increasing alkaline phosphatase activity and ECM mineralization. Additionally, RAW 264.7 osteoclastic cells cultured on ECM-functionalized surface exhibited higher tartrate-resistant acid phosphatase activity. Functionalization of nanostructured Ti surfaces created a favorable microenvironment for bone cell activity and bone remodeling, contributing to the development of smart osseointegrated Ti implants.</p>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"114 8","pages":""},"PeriodicalIF":3.8,"publicationDate":"2026-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.a.70131","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148553440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reduced Complement and Granulocyte Activation by α-Gal Knockout Porcine Pericardium for Bioprosthetic Heart Valves: A Pilot Hemocompatibility Study","authors":"Claudia Dittfeld,&nbsp;Elisabeth Kemter,&nbsp;Barbara Kessler,&nbsp;Eckhard Wolf,&nbsp;Anett Jannasch,&nbsp;Claudia Sperling,&nbsp;Sems-Malte Tugtekin,&nbsp;Manfred F. Maitz","doi":"10.1002/jbm.a.70114","DOIUrl":"10.1002/jbm.a.70114","url":null,"abstract":"<p>Xenogeneic pericardium is the main source for biological aortic valve prostheses. Immune responses to α-Gal epitopes and free aldehyde groups after glutaraldehyde (GA) fixation are responsible for the graft deterioration and the need for reoperation. Genetically modified α-Gal-depleted porcine pericardium is evaluated in this pilot study as an alternative to avoid immunologic interactions and prevent degeneration. The absence of α-Gal epitopes was confirmed by both anti-α-Gal and IB4 isolectin staining in α-Gal-KO pericardia. Elastic Moduli, collagen and elastin composition, and in vitro calcification susceptibility were similar irrespective of the pericardium donor genotype. For testing the response of an α-Gal-negative recipient organism, incubation assays with human whole blood were performed. Native α-Gal-KO pericardium induced a lower inflammatory blood response than tissue without modification, regarding complement granulocyte activation. GA fixation enhanced the inflammatory response for both tissue types, but the lower granulocyte activation persisted for the α-Gal-KO tissue. Thrombogenicity, regarding coagulation cascade and platelet activation, was not affected by the genetic modification. Native tissues induced high activation of the coagulation cascade, attributed to tissue factor activity, which was abrogated by the GA fixation. Cell and fibrin deposits on the pericardia corroborated the blood phase observations. It is concluded that the distinct lower inflammatory potential of the α-Gal-KO pericardium makes it an attractive tissue for artificial heart valves; however, it requires a preparation method with sufficient inactivation of procoagulant tissue factor activity without providing an inflammatory stimulus. Prospectively, the removal of additional xeno-epitopes like Neu5Gc can be considered to reduce the inflammatory potential further.</p>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"114 7","pages":""},"PeriodicalIF":3.8,"publicationDate":"2026-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.a.70114","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148451065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"pH-Sensitive Cationic Liposomes for Oridonin and siRNA Co-Delivery to Inhibit Triple-Negative Breast Cancer","authors":"Fangfang Wang,&nbsp;Qiong Yang,&nbsp;Xiaoliang Chen,&nbsp;Jun Wu,&nbsp;Xianzhi Guo","doi":"10.1002/jbm.a.70108","DOIUrl":"10.1002/jbm.a.70108","url":null,"abstract":"<div>\u0000 \u0000 <p>To investigate the therapeutic efficacy of co-delivering a small molecule compound and an interfering RNA in triple-negative breast cancer (TNBC), a disease characterized by poor prognosis, this study developed pH-sensitive nanocomposite cationic liposomes for the simultaneous delivery of oridonin and siCX26. CX26 (Connexin 26), a gap junction protein associated with stem-like properties and chemoresistance, was targeted to enhance therapeutic efficacy against this aggressive subtype. Oridonin liposomes were prepared using thin-film dispersion followed by hydrated ultrasonication. Subsequently, siCX26 was incorporated and modified with carboxymethyl chitosan (CMCS) to produce pH-sensitive nanocomposite cationic liposomes (CMCS-Ori-siCX26-LipoPH⁺). Liposome stability was assessed via particle size analysis and zeta-potential measurement. TUNEL staining and western blotting were performed to evaluate apoptosis and related intracellular signaling. The effect of CMCS-Ori-siCX26-LipoPH⁺ on tumor apoptosis was further assessed in animal models. CMCS-Ori-siCX26-LipoPH⁺ preserved siRNA integrity for up to 8 h and maintained stable particle size for up to 12 days. TUNEL staining and western blotting showed that the liposomes significantly enhanced apoptosis in MDA-MB-231 breast cancer cells at extracellular pH 6.5. Moreover, the liposomes delivered siRNA into the cytoplasm while protecting it from lysosomal degradation. Animal studies confirmed that CMCS-Ori-siCX26-LipoPH⁺ inhibited tumor growth and induced tumor cell apoptosis in nude mice. The CMCS-Ori-siCX26-LipoPH⁺ liposome system effectively delivers oridonin to TNBC, inducing tumor cell apoptosis.</p>\u0000 </div>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"114 7","pages":""},"PeriodicalIF":3.8,"publicationDate":"2026-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148414507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Superelastic Ti-Zr-Nb-Sn Thin Films Fabricated by Magnetron Sputtering: Biocompatibility and Bacterial Biofilm Formation Assessment for Orthopedic Applications","authors":"Hiroki Chigama,&nbsp;Thibaud Choquet,&nbsp;Pascale Vigneron,&nbsp;Doïna Gordin,&nbsp;Anny Michel,&nbsp;Romeu Chelariu,&nbsp;Ramona Cimpoeșu,&nbsp;Antoine Fayeulle,&nbsp;Jean-François Grosset,&nbsp;Ulysse Pereira,&nbsp;Thierry Gloriant,&nbsp;Grégory Abadias,&nbsp;Amélie Fillon,&nbsp;Muriel Vayssade","doi":"10.1002/jbm.a.70119","DOIUrl":"10.1002/jbm.a.70119","url":null,"abstract":"<div>\u0000 \u0000 <p>Nickel-titanium (NiTi) alloys are widely used in orthopedics because of their excellent mechanical properties; however, hypersensitivity remains a concern because of Ni ion release during long-term implantation. To address this issue, we manufactured Ti-Zr-xNb-Sn thin films (<i>x</i> = 10, 15, 17, 20 at.%) using magnetron-sputtering, intended as coatings for NiTi devices. Given the superelasticity of Ti-Zr-Nb-Sn alloys, these coatings are expected to not only suppress the Ni ion release from the NiTi substrate but also reduce the formation of cracks in the coating by accommodating the substrate's deformation. The biocompatibility of the coatings was evaluated both in vitro and in vivo, as well as the potential risk of forming bacterial biofilms. Ti-Zr-xNb-Sn coatings (<i>x</i> = 15, 17 at.%) promoted pre-osteoblast differentiation compared to uncoated NiTi, without increasing the risk of biofilm formation by <i>Staphylococcus epidermidis</i>. The coated NiTi wires were implanted subcutaneously in mice for 28 days, and no strong rejection reaction was observed compared to uncoated NiTi. Additionally, the coatings showed superior corrosion resistance, indicating improved long-term stability. The deposition angle during sputtering influenced cell differentiation, suggesting that both chemical composition and surface morphology contribute to osteogenic responses. Overall, Ti-Zr-Nb-Sn coatings are a promising surface modification strategy for NiTi orthopedic implants.</p>\u0000 </div>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"114 7","pages":""},"PeriodicalIF":3.8,"publicationDate":"2026-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148392751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Edible Sulfonated Soy Protein Microcarriers for Cultivated Meat Cell Expansion","authors":"Andrea C. Filler,&nbsp;Alex S. Kermani,&nbsp;Aurora Duan,&nbsp;Shierly W. Fok,&nbsp;J. Kent Leach","doi":"10.1002/jbm.a.70116","DOIUrl":"10.1002/jbm.a.70116","url":null,"abstract":"<p>Microcarriers are essential in large-scale cell expansion for applications such as cultivated meat but are typically composed of non-edible materials, requiring additional cell retrieval steps. The ability to incorporate microcarriers into a final product would reduce cost and potentially add nutritional value. Additionally, substrate biophysical properties influence muscle cell adhesion and proliferation. We developed edible, functionalized soy microcarriers by sulfonating soy protein isolate (sSPI). Sulfonation introduced sulfonate groups in a controllable manner, enhancing sSPI conductivity and water uptake. Myoblasts adhered to soy protein gels in serum-free conditions, and cell adhesion on sulfonated and non-sulfonated soy substrates was mediated by integrin β1. Additionally, we interrogated the ability of sulfonated soy protein to mimic growth factor binding characteristic of sulfated glycosaminoglycans in the extracellular matrix. Sulfonated soy microcarriers adsorbed 17-fold more basic fibroblast growth factor (bFGF) than non-sulfonated counterparts after 24 h. Sulfonated soy microcarriers accelerated proliferation of primary bovine satellite cells at early timepoints compared to cells on Cytodex 3 microcarriers and supported cell expansion over 5 days, similar to unmodified soy protein microcarriers. Collectively, this study demonstrates that sulfonation induces physiochemical changes in soy protein, generating a functionalized substrate that can be used to fabricate microcarriers for satellite cell expansion and improved growth factor retention.</p>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"114 7","pages":""},"PeriodicalIF":3.8,"publicationDate":"2026-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.a.70116","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148392740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ROS-Responsive Quercetin Nanoparticles Improve the Prognosis of Traumatic Brain Injury by Inhibiting Aberrant Nrf2-Keap1 Signaling Pathway Activation","authors":"YiFan Lei,&nbsp;Rongrong Dou,&nbsp;Cuihuan Ma,&nbsp;Yan Fang,&nbsp;Zhichao Wang,&nbsp;Yu Gao,&nbsp;Chang Cai,&nbsp;Xiefeng Wang,&nbsp;Yongkang Zhang,&nbsp;Xianhua Fu","doi":"10.1002/jbm.a.70123","DOIUrl":"10.1002/jbm.a.70123","url":null,"abstract":"<div>\u0000 \u0000 <p>Traumatic brain injury (TBI) is one of the leading causes of mortality and disability worldwide, with secondary injury recognized as a critical therapeutic target. Quercetin (QR), a natural flavonoid, exerts antioxidant and anti-inflammatory effects by modulating the Nrf2–Keap1 pathway and shows neuroprotective potential in various neurological disorders. In this study, network pharmacology analysis identified 496 overlapping targets of QR and TBI, further highlighting the pivotal role of the Nrf2–Keap1 pathway in TBI treatment. However, the poor blood–brain barrier (BBB) permeability and low bioavailability of QR hinder effective brain-targeted delivery and limit its clinical translation. To address these challenges, we developed CAQK peptide-modified, reactive oxygen species (ROS)-responsive nanoparticles (C-PPS/Q), using PPS₁₂₀ as the core for targeted QR delivery. C-PPS/Q exhibited ROS-triggered QR release, significantly enhanced HT22 cell uptake in vitro, reduced ROS levels and apoptosis. In a TBI mouse model, C-PPS/Q specifically accumulated at the lesion site, prolonged the half-life of QR, demonstrated excellent biocompatibility, preserved BBB integrity, attenuated neuroinflammation, inhibited aberrant Nrf2-Keap1 pathway activation, and markedly improved neurological function. Collectively, C-PPS/Q nanoparticles effectively mitigate secondary brain injury after TBI and represent a promising brain-targeted therapeutic strategy for TBI management.</p>\u0000 </div>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"114 7","pages":""},"PeriodicalIF":3.8,"publicationDate":"2026-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148383414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cellular Insights Into Proangiogenic Activation in Fibroblast and Endothelial Cells by Dual Drug-Loaded Emulsion Electrospun Nanofibers for Enhanced Tissue Regeneration","authors":"Vinayagamurthy Kalaiselvi,&nbsp;Babu Liya,&nbsp;Yalavarthi Karthika,&nbsp;Kumar Deena Praveena,&nbsp;Stephen Nimish Mol,&nbsp;Anandasadagopan Suresh Kumar,&nbsp;Niraikulam Ayyadurai,&nbsp;Numbi Ramudu Kamini,&nbsp;Ponesakki Ganesan","doi":"10.1002/jbm.a.70120","DOIUrl":"10.1002/jbm.a.70120","url":null,"abstract":"<div>\u0000 \u0000 <p>Emulsion electrospinning is one of the versatile methods that offer high potential encapsulation and delivery of active ingredients within the fibrous scaffolds for various applications. In this study, an efficient nanofibrous core-shell biomaterial was fabricated for the restoration of damaged tissue using a polycaprolactone/polyvinyl alcohol-based emulsion loaded with β-carotene and keratin hydrolysate (KH) by electrospinning. The fabricated nanofiber was examined for its morphological, physicochemical, thermal, and mechanical properties. An in vitro drug release study was performed to reveal the release profiles of incorporated β-carotene and KH. The antioxidant capacity of the nanofiber was measured by DPPH and ABTS assays. The effect of nanofibers on cell morphology, proliferation, migration, adhesion, and angiogenic potential was examined using fibroblast (NIH/3 T3) and endothelial (EA.hy926) cells. The results showed that the emulsion electrospun fiber loaded with β-carotene and KH had a diameter of 249 nm, and the fibrous mat had a contact angle of 39.92°. It exhibited tensile strength of 2.05 MPa and an elongation at break value of 23.55%. A prolonged release of β-carotene and a burst release of KH from the nanofiber were noticed in the drug release study. Interestingly, nanofibers incorporated with β-carotene and KH exerted improved cell proliferation, migration, cell adhesion, and tube formation. It supported the expression of genes associated with angiogenic signaling in both fibroblast and endothelial cells. In conclusion, this study highlights the potential of electrospun nanofibers derived from emulsions for tissue regeneration, and it could be employed for accelerated wound repair when infused with β-carotene and KH.</p>\u0000 </div>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"114 7","pages":""},"PeriodicalIF":3.8,"publicationDate":"2026-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148383382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biomimetic Collagen Scaffolds Natural Cross-Linking Strategies via Transglutaminase and Methylglyoxal for Skin Repair","authors":"Mina Vaez,&nbsp;Matthew Yen,&nbsp;Aiman Ali,&nbsp;Sophia Huang,&nbsp;Liyang Zhong,&nbsp;Christina M. Schuh,&nbsp;Laurent Bozec","doi":"10.1002/jbm.a.70104","DOIUrl":"10.1002/jbm.a.70104","url":null,"abstract":"<p>Collagen-based scaffolds are widely used in tissue engineering for skin repair due to their biocompatibility and structural resemblance to extracellular matrix. However, rapid degradation and insufficient mechanical stability limit their effectiveness. This study investigates natural cross-linking strategies utilizing Transglutaminase (TG) and Methylglyoxal (MGO) to enhance scaffold durability while maintaining biocompatibility. Monolayer and bilayer collagen scaffolds were fabricated and cross-linked using enzymatic (TG) and non-enzymatic (MGO) treatments. Cross-linking efficiency was assessed via TNBS/Hydroxyproline assays, while AFM and mechanical testing evaluated structural and mechanical properties. Cell viability (LIVE/DEAD), metabolic activity (MTS assay), and cell migration (in-growth and out-growth assays) were analyzed to assess biocompatibility and scaffold functionality. Wound healing was also assessed histologically. TG and MGO crosslinking significantly enhanced collagen fibril organization, scaffold stiffness, and enzymatic resistance. TG- and MGO-treated scaffolds exhibited a homogeneous fibrillar structure with improved mechanical integrity. Both cross-linking strategies supported high fibroblast viability and sustained metabolic activity over time. Migration assays confirmed that TG and MGO scaffolds facilitated fibroblast infiltration and wound closure. In vivo, the bilayer scaffolds with TG/MGO cross-linking showed full epithelialization, with the Tg/25 MGO group exhibiting reduced inflammation, organized collagen, and hair follicle formation, suggesting improved regeneration. These findings highlight the potential of biomimetic collagen scaffolds as wound dressings for dermal tissue regeneration and skin repair.</p>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"114 7","pages":""},"PeriodicalIF":3.8,"publicationDate":"2026-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.a.70104","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148378852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Granular Hydrogel Composites for Noninvasive Optical Biosensing","authors":"Tyrell J. Williams,&nbsp;Waqas Saleem,&nbsp;Sujeong Ahn,&nbsp;David Chimene,&nbsp;Michael J. McShane,&nbsp;Daniel L. Alge","doi":"10.1002/jbm.a.70121","DOIUrl":"10.1002/jbm.a.70121","url":null,"abstract":"<p>Granular hydrogels are attracting increasing attention for tissue engineering and regenerative medicine applications, owing to their highly tunable physical characteristics, exceptional modularity, and naturally interconnected microporosity. These features are also attractive for next-generation biosensors that better integrate with the body. To apply the granular hydrogel paradigm to biosensor development, porphyrin-containing chemo-optical oxygen- and glucose-biosensing microdomains were encapsulated within poly(ethylene glycol) (PEG) hydrogel microparticles (80.8 ± 37.0 μm), which were fabricated using thiol-vinyl sulfone chemistry and a batch emulsification. Importantly, the granular hydrogel system demonstrated shear thinning capabilities and were annealed with a PEG-tetra-thiol linker to form granular hydrogels. This granular hydrogel platform possessed an average porosity of 25.0% ± 6.6% and a low shear storage modulus of 1448.7 ± 708.3 Pa, indicating a low crosslinking density and large mesh size, which permits analyte diffusion to the sensing microdomains. In vitro testing of granular hydrogels loaded with oxygen-biosensing microdomains demonstrated responsiveness from 0% to 21% oxygen and a Stern-Volmer quenching constant of 0.0131 ± 0.0003 μM⁻¹. Additionally, in vitro testing of granular hydrogels with glucose-biosensing microdomains under a physiologically relevant oxygen concentration demonstrated a glucose sensitivity of 0.308 ± 0.068 μs·dL/mg, and a sensing range of detection from 32.8 ± 18.0 to 180.9 ± 17.8 mg/dL, which spans from hypoglycemia to mild hyperglycemia. Furthermore, biosensing performance was maintained after electron-beam sterilization. In summary, these results demonstrate proof-of-concept for integrating chemo-optical biosensors into granular hydrogels as well as the ability to measure physiologically relevant fluctuations in oxygen and glucose.</p>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"114 7","pages":""},"PeriodicalIF":3.8,"publicationDate":"2026-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.a.70121","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148371350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tunable Sol–Gel Transition in Poloxamer Blends for Injectable Osteoarticular Applications","authors":"Marta Tuszynska,&nbsp;Joanna Skopinska-Wisniewska,&nbsp;Kaoutar Chattahy,&nbsp;Aliaa Karam,&nbsp;Kyle Storey,&nbsp;Anna Ryl,&nbsp;Daniel J. Kelly,&nbsp;Anna Bajek","doi":"10.1002/jbm.a.70097","DOIUrl":"10.1002/jbm.a.70097","url":null,"abstract":"<p>Thermoresponsive poloxamers are commonly used in biomedical applications; however, their wider translational applicability depends on precisely controlling sol–gel transition behavior near body temperature while ensuring injectability and a favorable biocompatibility profile. In this study, blends of three clinically relevant poloxamers were evaluated to find formulations suitable for potential injectable meniscal and joint therapies. The rheological properties and temperature-dependent gelation of these formulations were characterized, followed by testing their injectability into meniscal defects and assessing biological responses in vitro. The gelation temperatures reported in this work were operationally defined from viscosity-based temperature sweep measurements. Biocompatibility, cell viability, and migration of outer fibrochondrocytes exposed to selected poloxamer hydrogels were examined to gauge their potential for orthopedic applications. Different material behaviors emerged based on polymer composition. Synperonic F-108 combined with Poloxamer 188 showed decreased adhesiveness and aggregation, along with maintained cell viability after prolonged incubation, indicating potential as a coating material or a general structural matrix. Conversely, blends of Kolliphor K 407 with Poloxamer 188 displayed temperature-dependent increases in viscosity. They formed stable gels at body temperature, maintaining a favorable biological response in direct-contact conditions, which supports their further evaluation in long-term injectable applications with controlled structural stability. These results show that careful selection and optimization of poloxamer blends allow tailoring material properties for specific biomedical functions. The adjustable thermoresponsive behavior, chemical inertness, and simple preparation of these triblock copolymers make them practical for further development in injectable biomaterials and drug delivery systems for osteoarticular repair.</p>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"114 7","pages":""},"PeriodicalIF":3.8,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.a.70097","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148364172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}