Journal of biomedical materials research. Part A最新文献

{"title":"Biological Responses and Hemocompatibility of Diamond-Like Carbon Thin Films on Different PEO Interlayers for Potential Cardiovascular Stent Applications","authors":"Nasir Shahin,&nbsp;Morteza Shamanian,&nbsp;Mahshid Kharaziha","doi":"10.1002/jbm.a.37953","DOIUrl":"https://doi.org/10.1002/jbm.a.37953","url":null,"abstract":"<div>\u0000 \u0000 <p>Plasma electrolytic oxidation (PEO) considerably affects controlling the degradation rate of magnesium-based implants to approach the healing period. However, the biological properties still require further improvement, particularly for blood-contact applications, such as cardiovascular stents. This research aims to study in vitro biological properties of the duplex diamond-like carbon (DLC)/plasma electrolytic oxidation (PEO) coatings as a function of various PEO middle layers for potential cardiovascular stent applications. To this aim, two different PEO coatings including silicate and phosphate compounds were applied on AZ31 substrate as middle-layers, and a top DLC layer with 1 μm thickness was successfully synthesized on them. Moreover, the role of different PEO interlayers on the degradation behavior, biocompatibility, hemocompatibility, and its mechanism are studied. Results showed a considerable decrease in degradation rate after applying the PEO process and the PEO-Ph revealed the optimized degradation performance in just PEO-coated samples. On the other side, the best degradation performance between duplex-coated samples was obtained for DLC/PEO-Si according to its higher diamond-like structure. Moreover, the viability of human umbilical vein endothelial cells on DLC/PEO-Ph was higher than that of the DLC/PEO-Si, which might be attributed to higher protein adsorption on its surface. In the case of hemocompatibility, a considerable decrease in hemolysis ratio along with remarkable improvement in clotting behavior was observed by applying the PEO process. However, the hemolysis ratio was reduced as being safe for blood-contact applications just for duplex-coated samples. In conclusion, a promising coating for blood-contact applications based on DLC/PEO in particular in the case of DLC/PEO-Si has been provided in this study.</p>\u0000 </div>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 7","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144536962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Strontium-Loaded Titanium and Systemic Teriparatide Synergistically Enhance Osteogenesis and Osseointegration in an Osteoporotic Rabbit Model","authors":"Koichiro Shima,&nbsp;Takayoshi Shimizu,&nbsp;Seiji Yamaguchi,&nbsp;Bungo Otsuki,&nbsp;Toshiyuki Kawai,&nbsp;Yaichiro Okuzu,&nbsp;Yusuke Takaoka,&nbsp;Norimasa Ikeda,&nbsp;Shuichi Matsuda","doi":"10.1002/jbm.a.37960","DOIUrl":"https://doi.org/10.1002/jbm.a.37960","url":null,"abstract":"<div>\u0000 \u0000 <p>Poor osseointegration of Titanium (Ti) implants in osteoporotic bone can lead to early construct failure in clinical settings. This study investigated whether combining Strontium (Sr) surface loading (modified alkali and heat treatment) with systemic teriparatide administration could enhance implant osseointegration in osteoporotic conditions. Mouse osteoblast-like cells (MC3T3-E1) were cultured on Sr-loaded Ti surfaces with and without teriparatide administration to evaluate cell adhesion, proliferation, differentiation, and mineralization capacity. This in vivo study utilized an osteoporotic rabbit femur through ovariectomy and steroid administration. The combined treatment (Sr-loaded Ti and teriparatide) enhanced osteoblast differentiation and mineralization in vitro, with an increase in alkaline phosphatase activity and alizarin red staining. Six and twelve weeks after in vivo implantation, the combination therapy demonstrated superior outcomes compared to the single treatments (Sr-loaded Ti or teriparatide), including enhanced bone–implant interfacial strength, improved bone morphology parameters, higher mineral apposition rates, and greater bone–implant contact. These findings demonstrate that the synergistic approach of Sr-loaded Ti implants combined with systemic teriparatide administration considerably improves implant osseointegration in osteoporotic bone, suggesting a promising strategy for enhancing implant outcomes in patients with osteoporotic bone quality.</p>\u0000 </div>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 7","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144550945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multidimensional Applications and Challenges of Metal–Organic Frameworks (MOFs) in Biomedicine: From Drug Safety Evaluation to Drug Delivery","authors":"Zhe Liu,&nbsp;Yuanye Gao,&nbsp;Wenyuan Shao,&nbsp;Xuhui Guo,&nbsp;Danqing Zhao,&nbsp;Rui Yang,&nbsp;Siwei Li","doi":"10.1002/jbm.a.37952","DOIUrl":"https://doi.org/10.1002/jbm.a.37952","url":null,"abstract":"<div>\u0000 \u0000 <p>Metal–organic frameworks (MOFs) are porous materials composed of metal ions or clusters and organic ligands connected through coordination bonds, exhibiting high specific surface areas, tunable pore structures, and excellent chemical stability. These unique features have enabled MOFs to be widely applied in catalysis, gas separation, and environmental purification. In recent years, the potential of MOFs in the biomedicine field has garnered significant attention. MOFs offer efficient drug loading and controlled release capabilities, particularly for targeted therapies in oncology and other diseases. Furthermore, their structural versatility positions them as promising candidates for antitumor and antibacterial treatments. However, challenges related to biocompatibility, in vivo degradation, and potential toxicity need further investigation. This review explores the latest advancements in MOF applications in biomedicine, focusing on drug delivery, targeted therapy, and modification strategies, as well as toxicity assessment and mechanisms. Finally, future research directions are proposed, including the development of intelligent drug delivery systems, multimodal diagnostic platforms, and optimized MOF designs for clinical translation.</p>\u0000 </div>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 7","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144492953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Iridium-Bismuth-Oxide Coatings for Use in Neural Stimulating Electrodes: The Influence of Ir/Bi Ratio","authors":"Xingge Xu,&nbsp;Irshad Ali,&nbsp;Sandra Minotti,&nbsp;Heather D. Durham,&nbsp;Sasha Omanovic","doi":"10.1002/jbm.a.37956","DOIUrl":"https://doi.org/10.1002/jbm.a.37956","url":null,"abstract":"<p>Implantable neural prosthetics with stimulating electrodes are increasingly employed in medical practices to treat neural disabilities. The electrode material is expected to provide high charge storage and injection capacity (CSC/CIC) and low impedance for safe, efficient, and precise neural stimulation, while at the same time, being small. To improve the current state-of-the-art neural-electrode material, iridium oxide (IrOx), Ir_mBi_1-mOx coatings of various compositions (<i>m</i> = 0, 0.2, 0.4, 0.6, 0.8, and 1.0) produced by thermal deposition were evaluated. The Ir_0.8Bi_0.2Ox yielded a CSC of 17.7 ± 1.1 mC/cm², which is four-fold higher than that of IrOx. At the same time, the impedance of Ir_0.8Bi_0.2Ox at 1 kHz was measured to be half of that of IrOx. The superior performance of Ir_0.8Bi_0.2Ox was explained by forming amorphous structures that facilitate the intercalation of H⁺ and OH⁻ ions into deeper oxide structures that contribute to faradaic charge storage. The Ir_0.8Bi_0.2Ox electrode also showed good stability and biocompatibility, which makes it potentially a good candidate for neural stimulating electrodes.</p>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 7","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.a.37956","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144503130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis of Chondroitin Sulfate Conjugated Nanofiber Shish Kebabs as a Biomimetic Bone Template","authors":"Tony Yu,&nbsp;Paul DeSantis,&nbsp;Seyong Kim,&nbsp;Christopher Y. Li,&nbsp;Michele Marcolongo","doi":"10.1002/jbm.a.37955","DOIUrl":"https://doi.org/10.1002/jbm.a.37955","url":null,"abstract":"<div>\u0000 \u0000 <p>Synthetic polymeric bone grafts have emerged as a promising strategy for bone tissue engineering. Polycaprolactone (PCL) nanofiber shish kebab (NFSK) templates were fabricated as synthetic bone scaffolds via polymer crystallization of a block copolymer (BCP) of PCL-<i>b</i>-polyacrylic acid (PAA). The BCP-functionalized NSFKs provide a unique template that allows for the spatial and orientational control of the nanosized mineral crystals in the PAA anionic galleries, mimicking the molecular structure of bone. The objective of this study was to use this platform to design biomimetic bone templates by modifying the surface with biomimetic functional groups. As a result, chondroitin sulfate (CS) was conjugated onto the kebabs via 1-ethyl-3-(-3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide (EDC/NHS) crosslinking of the CS terminal amine group and the PAA carboxylic acid group. Fourier-transform infrared spectroscopy (FTIR) and mass balance showed the formation of an amide bond and an increase in mass after conjugation. MC3T3 E1 pre-osteoblast cells were cultured on the CS-NFSK templates and showed that the presence of CS promoted alkaline phosphatase (ALP) activity and cell proliferation. Osteogenic gene expression, including <i>RUNX2</i>, <i>ALP</i>, <i>COL1,</i> and <i>BGLAP,</i> were upregulated in the CS-NFSK templates. For the first time, CS-NFSK was molecularly engineered to mimic the bone structure and matrix, showing promise as a biomimetic bone template.</p>\u0000 </div>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 7","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144482234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Coaxial Bioprinting of Schwann Cells and Neural Stem Cells in a Three-Dimensional Microenvironment for the Repair of Peripheral Nerve Defects","authors":"Xuanzhi Wang,&nbsp;Tao Xu,&nbsp;Fei Wang","doi":"10.1002/jbm.a.37943","DOIUrl":"https://doi.org/10.1002/jbm.a.37943","url":null,"abstract":"<div>\u0000 \u0000 <p>Currently, autologous nerve (AN) transplantation remains the gold standard for treating peripheral nerve injuries (PNIs). However, its inherent limitations, including donor site morbidity and immune rejection risks associated with allografts, have prompted the exploration of alternative therapeutic strategies. Among these, tissue engineering approaches have gained significant attention, with nerve conduit design emerging as a particularly promising research direction. Electrospinning technology has been widely adopted for its ability to fabricate nanofibrous scaffolds that closely mimic the native extracellular matrix. In this study, we engineered an aligned nanofiber conduit utilizing polylactic acid and gelatin through electrospinning, and integrated a sodium alginate hydrogel enriched with Schwann cells (SCs) and neural stem cells (NSCs) via coaxial bioprinting. The three-dimensional (3D) hydrogel microenvironment facilitated synergistic interactions between SCs and NSCs, augmenting the secretion of neurotrophic factors such as brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF). A dynamic perfusion culture system was further employed to optimize cell viability and functionality. In vivo studies revealed that the implantation of this conduit in a sciatic nerve defect model markedly enhanced motor function recovery, nerve regeneration, and muscle morphology. These improvements were substantiated by an increased sciatic functional index (SFI), heightened expression of S-100 and NF-200, and greater myelin thickness and axon diameter. Although the efficacy of the 3D-aligned nanofiber conduit cocultured with SCs and NSCs approximated that of AN transplantation, further research is imperative to identify more efficient seed cells and biocompatible 3D carriers to achieve optimal nerve regeneration. This study highlights the potential of tissue-engineered nerve conduits as a viable alternative for PNI repair, paving the way for future advancements in the field.</p>\u0000 </div>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 7","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144367421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing the Potency of Growth Factor-Mimicking Peptides via Cross-Presentation With Integrin Ligands","authors":"Sydney Neal,&nbsp;Xiaohong Tan,&nbsp;Era Jain,&nbsp;Charlotte Chen,&nbsp;Mohammadjafar Hashemi,&nbsp;Lori A. Setton,&nbsp;Nathaniel Huebsch","doi":"10.1002/jbm.a.37944","DOIUrl":"https://doi.org/10.1002/jbm.a.37944","url":null,"abstract":"<p>Growth factors enhance survival and integration of transplanted Mesenchymal Stromal Cells (MSC), but successful supplementation often requires supraphysiological growth factor doses, risking off-target effects. Short peptide mimics like the knuckle epitope (KE) of Bone Morphogenetic Protein 2 (BMP-2) can be covalently immobilized to biomaterials, localizing bioactivity at the delivery site. However, these short peptides often lack the potency of full-length growth factors. We sought to improve the potency of alginate-grafted KE to encourage MSC osteogenic differentiation. When alginate gels co-presented KE and integrin-binding cyclo-RGD (cRGD) peptides, MSC expressed early markers of osteogenesis (Runt-related Transcription Factor2, RUNX2, Alkaline Phosphatase, ALP, and osteocalcin, OCN) in a KE-dose dependent manner. When co-presented with cRGD, high concentrations of KE partially mimicked the osteogenic potential (ALP induction) of full-length BMP-2. Proximity between KE and cRGD may be the mechanism through which high dose KE induces osteogenesis in the presence of cRGD. To investigate this possibility, we used orthogonal strain-promoted azide-alkyne cycloaddition (SPAAC) and maleimide-thiol chemistries to graft KE and cRGD in a bivalent (same alginate chain) and a monovalent (different alginate chain) manner, at constant bulk peptide concentration. Bivalent presentation of peptides (separation distance of 5.5 ± 0.5 nm verified by FRET) ultimately increased RUNX2 and ALP expression compared to monovalent presentation. This platform technology can be used in future studies to control peptide nanopatterning to enhance potency, in the context of MSC-based therapies and beyond.</p>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 7","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.a.37944","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144367420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to “Injectable Hydrogel Scaffold Incorporating Microspheres Containing Cobalt-Doped Bioactive Glass for Bone Healing”","authors":"","doi":"10.1002/jbm.a.37910","DOIUrl":"https://doi.org/10.1002/jbm.a.37910","url":null,"abstract":"<p>\u0000 <span>Ghiasi Tabari, P</span>, <span>Sattari, A</span>, <span>Mashhadi Keshtiban, M</span>, <span>Karkuki Osguei, N</span>, <span>Hardy, JG</span>, <span>Samadikuchaksaraei, A.</span> <span>Injectable Hydrogel Scaffold Incorporating Microspheres Containing Cobalt-Doped Bioactive Glass for Bone Healing</span>. <i>J Biomed Mater Res A.</i> <span>2024</span>, <span>112</span>(<span>12</span>): <span>2225</span>–<span>2242</span>; doi: https://doi.org/10.1002/jbm.a.37773.\u0000 </p><p>In Figure 4, panel A-S-G7Co of Alizarin Red S staining in our original article, an incorrect figure was used. Figure 4 is now updated with new correct panel for A-S-G7Co of Alizarin Red S staining. Please note that this correction does not affect the results, the description and interpretation of the results, or the conclusions of the article.</p><p>We apologize for this error.</p>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 7","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.a.37910","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144367422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrochemical Assessment of the Galvanic Corrosion and Metal Ion Release in Overlapping Stent and Vascular Plug Systems","authors":"O. Burak Istanbullu,&nbsp;Gulsen Akdogan,&nbsp;Halis Yilmaz,&nbsp;Mustafa Istanbullu","doi":"10.1002/jbm.a.37946","DOIUrl":"https://doi.org/10.1002/jbm.a.37946","url":null,"abstract":"<p>Cardiovascular diseases cause the highest global mortality rates and are often treated with surgical interventions such as stent or vascular plug placement. However, in-stent restenosis develops over time depending on the material composition and interactions with body fluids. Current strategies to address restenosis include balloon angioplasty or placing a secondary stent at the same site. A key concern with overlapping stents is the increasing risk of galvanic corrosion, as most cardiovascular stents have metallic composition. This study examines galvanic corrosion rates in different vascular stent and plug combinations using electrochemical corrosion characterization techniques. Three metallic vascular specimens with varying compositions are evaluated. The specimens are immersed in simulated body fluid at 37°C under individual and overlapping conditions. Electrochemical impedance spectroscopy and current density measurements, conducted via potentiostat, provide insights into the corrosion behavior of each specimen configuration. Additionally, inductively coupled plasma mass spectrometry quantifies metal ion release through SBF samples. Results show that combining dissimilar materials in overlapping placements significantly increases galvanic corrosion and metal ion release. The corrosion current density (<i>i</i>_corr) significantly increased from 11.75 μA/cm² in the individual bare-metallic stent to 522.3 μA/cm² in the stent-on-plug configuration. A similar increase was observed in the stent-on-stent configuration, with an <i>i</i>_corr of 132.6 μA/cm². These results corresponded with notable decreases in electrochemical impedance and polarization resistance, measured as low as 0.039 kΩ (<i>Z</i>_T) and 0.057 kΩ cm² (<i>R</i>_P) for the stent-on-plug system. Consequently, the calculated corrosion rate escalated to 2254 μm/year, with a mass loss reaching 42.22 mg/cm²·year. ICP-MS analysis supported these findings, showing the highest levels of metal ion release in the stent-on-plug configuration, with 23.86 ppm of Ni and 0.41 ppm of Cr. These findings highlight the importance of stent-material selection in reducing corrosion-related complications. Implementing material-specific strategies in secondary stent placement can lower the risks of inflammatory host response, stent failure, and their long-term effects.</p>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 6","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.a.37946","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144292931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tendon-Tissue Derived Monofilaments by Electrochemical Compaction: Production and Characterization","authors":"Phillip McClellan,&nbsp;Joohee Choi,&nbsp;Mikhail Nasrallah,&nbsp;Kathleen Lundberg,&nbsp;Ozan Akkus","doi":"10.1002/jbm.a.37948","DOIUrl":"https://doi.org/10.1002/jbm.a.37948","url":null,"abstract":"<p>Repair of tendon tissues remains a complex problem in orthopedic surgery. Tendon auto- and allografts are not utilized to the full extent of their capabilities due largely to the lack of porosity and availability of properly processed tendon stock. Cryomilling is often utilized to maximize surface area-to-volume while limiting alterations to native protein/gene structure. In this study, native tendons were isolated, cryomilled, and decellularized using a truncated protocol. The resulting decellularized tendon powder exhibited reduced DNA content of less than 15 ng/mg, indicating effective removal of cellular components. The resulting decellularized tendon “powder” was then subjected to mild acidic conditions to partially solubilize the collagen within the extracellular matrix to produce a solution that could be electrochemically compacted to generate aligned fibers. Proteomic analyses revealed the presence of tendon-related proteins (cartilage oligomeric protein, fibromodulin, lumican, biglycan, and tenascin c). Proteoglycans were present in tendon-derived thread (TDT) and largely absent in pure collagen threads, as visualized by safranin O and quantified by dimethylmethylene blue staining. Mesenchymal stem cells seeded and cultured for up to 14 days on collagen threads and TDTs exhibited similar expression of genes related to tendon tissue.</p>","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"113 6","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.a.37948","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144299867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}