Morin-Loaded Chitosan-Poloxamer Hydrogel as an Osteoinductive Delivery System for Endodontic Applications

IF 3.9 3区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of biomedical materials research. Part A Pub Date : 2025-03-14 DOI:10.1002/jbm.a.37895

Jesse Augusto Pereira, Victor Martin, Rita Araújo, Liliana Grenho, Pedro Gomes, Joana Marto, Maria Helena Fernandes, Catarina Santos, Cristiane Duque

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引用次数: 0

Abstract

Considering the search for new biocompatible intracanal medicaments that can preserve remaining cells and stimulate bone tissue repair in the periapical region, this study aimed to synthesize and characterize the physicochemical properties of morin-loaded chitosan-poloxamer hydrogel (MCP) as well as to evaluate its osteogenic potential. Morin hydrate (M) was loaded into chitosan-poloxamer (CP) hydrogel and the resulting particles were characterized by infrared spectroscopy (FTIR), UV–vis spectrophotometer and scanning electron microscopy. Biological assays evaluated the metabolic activity, cell morphology and alkaline phosphatase (ALP) activity of human bone marrow stem cells (HBMSC) in three different settings, such as the exposure to dissolved morin, hydrogel's leachates and assembled particles by indirect contact. Cells cultured in standard culture conditions were used as control. The effect of CP and MCP particles on the formation of collagenous and mineralized tissues was also assessed within the organotypic model of segmented embryonic chick femora. Datasets were assessed for one-way analysis of variance (ANOVA), followed by Tukey's post hoc test (p < 0.05). Morin at 50 μg/mL was cytocompatible and increased ALP activity. CP and MCP particles showed stability, and morin was entrapped in the hydrogel matrix without changing its chemical structure. Cultures treated with 30-min CP and MCP hydrogel leachates presented significantly higher metabolic activity compared to control. By indirect contact, CP particles increased metabolic activity, but only MCP particles induced an upregulation of ALP activity in comparison to control. The amount of collagenous tissue and mineralized area on the fractured embryonic chick femora was greater in MCP particles compared to CP counterparts. Chitosan-poloxamer platforms are suitable systems to delivery morin, enhancing cell proliferation and bone mineralization, which upholds its application as intracanal medication for endodontic purposes.

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负载莫里素的壳聚糖-波洛沙姆水凝胶作为根管应用的骨诱导递送系统

考虑到寻找新的生物相容性的管内药物，以保存剩余细胞并刺激根尖周围区域的骨组织修复，本研究旨在合成和表征负载桑嘌呤的壳聚糖-波洛沙姆水凝胶（MCP）的物理化学性质，并评估其成骨潜力。采用红外光谱（FTIR）、紫外-可见分光光度计（UV-vis）和扫描电镜（sem）对壳聚糖-波洛沙姆（CP）水凝胶进行表征。生物试验评估了人骨髓干细胞（HBMSC）在三种不同环境下的代谢活性、细胞形态和碱性磷酸酶（ALP）活性，例如暴露于溶解的桑里素、水凝胶的渗出液和间接接触的组装颗粒。以标准培养条件下培养的细胞为对照。在鸡分段胚胎股骨器官型模型中，研究了CP和MCP颗粒对胶原和矿化组织形成的影响。对数据集进行单因素方差分析（ANOVA），然后进行Tukey事后检验（p < 0.05）。50 μg/mL的桑苷具有细胞相容性，可提高ALP活性。CP和MCP颗粒表现出稳定性，桑里素被包裹在水凝胶基质中而不改变其化学结构。与对照组相比，经30分钟CP和MCP水凝胶浸出液处理的培养物代谢活性显著提高。通过间接接触，CP颗粒增加了代谢活性，但与对照组相比，只有MCP颗粒诱导ALP活性上调。MCP颗粒与CP颗粒相比，在断裂的胚胎鸡股骨上胶原组织的数量和矿化面积更大。壳聚糖-波洛沙姆平台是递送桑酸的合适系统，促进细胞增殖和骨矿化，这支持其作为根管目的的管内药物的应用。

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来源期刊

Journal of biomedical materials research. Part A 工程技术-材料科学：生物材料

CiteScore

10.40

自引率

2.00%

发文量

135

审稿时长

3.6 months

期刊介绍： The Journal of Biomedical Materials Research Part A is an international, interdisciplinary, English-language publication of original contributions concerning studies of the preparation, performance, and evaluation of biomaterials; the chemical, physical, toxicological, and mechanical behavior of materials in physiological environments; and the response of blood and tissues to biomaterials. The Journal publishes peer-reviewed articles on all relevant biomaterial topics including the science and technology of alloys,polymers, ceramics, and reprocessed animal and human tissues in surgery,dentistry, artificial organs, and other medical devices. The Journal also publishes articles in interdisciplinary areas such as tissue engineering and controlled release technology where biomaterials play a significant role in the performance of the medical device. The Journal of Biomedical Materials Research is the official journal of the Society for Biomaterials (USA), the Japanese Society for Biomaterials, the Australasian Society for Biomaterials, and the Korean Society for Biomaterials. Articles are welcomed from all scientists. Membership in the Society for Biomaterials is not a prerequisite for submission.