Journal of Bioactive and Compatible Polymers最新文献

{"title":"Exploring the efficacy of catha edulis extract-loaded nanofibrous scaffolds seeded with bone marrow-derived stem cells for diabetic wound healing: A preclinical investigation","authors":"Yinsen Song, Sisen Zhang, Tianli Fan, Zhenzhen Yang, Peiliang Li, Lu Zhang, Na Gao, Wei Mei","doi":"10.1177/08839115241248190","DOIUrl":"https://doi.org/10.1177/08839115241248190","url":null,"abstract":"Objectives:The aim of this study was to investigate the potential of incorporating catha edulis extract into polycaprolacton/gelatin scaffolds using electrospinning technique for the treatment of diabetic wounds in a rat model.Methods:The in vitro characterization of the scaffolds was performed using various assays, including anti-inflammatory assay, microstructure study, DPPH radical scavenging assay, cell viability assay, hemocompatibility assay, and bacterial penetration assays. The scaffolds were then seeded with bone marrow-derived stem cells and cultured before implantation into the rat model.Results:The results of the in vitro study showed that the produced scaffolds were nanofibrous, antioxidative, and non-toxic to skin cells. In vivo study demonstrated that the stem cell and catha edulis extract-loaded scaffolds had the highest rate of wound closure and histomorphometric parameters compared to other groups. Moreover, gene expression studies showed that the developed wound dressings increased the expression of VEGF gene and reduced the expression of glutathione peroxidase gene.Conclusion:These findings suggest that the catha edulis extract-loaded polycaprolacton/gelatin scaffolds could be a promising therapeutic option for diabetic wounds.","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2024-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140803022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comparative study of gelatin-based nanofiber and sponge wound dressings","authors":"Aysegul Uzuner-Demir, Z. B. Ahi, O. Polat, Fatih Arican, A. Sancakli","doi":"10.1177/08839115241241297","DOIUrl":"https://doi.org/10.1177/08839115241241297","url":null,"abstract":"Gelatin is a collagen-derived biopolymer with high biocompatibility, commonly used in the production of wound dressing materials. Gelatin-based wound dressings, obtained through different processing methods, come with their own advantages and disadvantages depending on their physical structure. In this study, the effects of gelatin wound dressing materials with two different morphologies on wound healing are investigated in detail. The study investigated that the preparation of gelatin-based wound dressing materials using two different methods, followed by their chemical characterization and examination of their effects on biocompatibility and wound healing. To comprehend the impact of nanofiber structure and porous sponge structure on the wound healing process, the chemical structure of the prepared wound dressings was analyzed using Fourier Transform Infrared Spectrophotometer (FTIR). The morphological structure was visualized through Scanning Electron Microscope (SEM), the thermal properties were determined using Thermogravimetric Analyzer (TGA) and Differential Scanning Calorimeter (DSC). Additionally, the in vivo wound healing effects of gelatin-based wound dressings with different structures were evaluated through macroscopic observation and histological analysis in a dorsal region wound-healing rat model, monitored for 21 days. It was observed that wound healing materials with desired nanofiber and porous sponge structures were successfully obtained based on the SEM analysis. The gelatin nanofiber dressing showed a randomly oriented, bead-free, and smooth morphology with an average fiber diameter of approximately 1250 ± 0.35 nm, while the crosslinked gelatin sponge dressing had pore sizes ranging from 20 to 160 μm, and interconnected pore structures. The chemical structure remained unaltered according to FTIR graphs and these results were further supported by DSC and TGA graphs. Results from in vitro biocompatibility studies indicated that the wound dressing materials were biologically compatible, non-toxic, and did not cause irritation or sensitivity to the skin. Regarding in vivo analysis, it was determined that gelatin dressing materials were functional in the wound healing process, without the use of any auxiliary materials.","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2024-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140677571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biocompatible sodium alginate–silk fibroin antibacterial microspheres from a microfluidic platform for infected wound repair","authors":"Jinglong Liu, Wei Zhou, Shuo Yang, Ruixue Chu, Yuqi Zhen, Rui Ding, Juan Xu, Zhiyong Qian, Ning Wen","doi":"10.1177/08839115241241294","DOIUrl":"https://doi.org/10.1177/08839115241241294","url":null,"abstract":"Micro/nano drug delivery systems can provide ideal controlled drug release. Microfluidic chip technology plays an important role in the preparation of microspheres. Sodium alginate (SA) has been used to prepare microspheres as drug carriers owing to its good biosafety and easy preparation. However, these microspheres lack antimicrobial activity and drug loading efficiency, which prevent their application for infected wound repair. Although silver nanoparticles (AgNPs) possess broad-spectrum antibiotic activity, liquid mixtures of AgNPs and SA are too unstable to fabricate drug-loaded microspheres using microfluidic chip technology. In this study, AgNPs were coated with silk fibroin (SF) and then dispersed in SA solution to fabricate antibacterial microspheres (denoted SA-SF-Ag) using microfluidic chip technology. SA-SF-Ag effectively inhibited the growth of microorganisms and gradually released AgNPs. Moreover, in vivo results showed that SA-SF-Ag promoted infected wound healing and angiogenesis by killing Pseudomonas aeruginosa on the surface of infected skin wounds of mouse models. This study offers a new method to integrate AgNPs into organic polymeric microspheres for the treatment of infected wounds.","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2024-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140585829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biocompatibility of insulin-PLA stereocomplex","authors":"Tovi Shapira-Furman, Abraham Nyska, Abraham J Domb","doi":"10.1177/08839115241241047","DOIUrl":"https://doi.org/10.1177/08839115241241047","url":null,"abstract":"Biocompatibility is essential for drug delivery systems to ensure safety. Poly(lactic acid) (PLA) and its copolymers with glycolic acid and caprolactone, are known as safe biodegradable implants and carriers of drugs in clinical use. These polymers have been clinically used for the delivery of peptides, such as: LHRH, somatostatin and growth hormone. While the safety of PLA has been confirmed, the biocompatibility of PLA- based stereocomplexes with peptides has not been investigated. Stereocomplex is a complex formed by two molecules with opposite enantiomeric configuration. D-PLA consists of a chiral monomer thus can adopts a three dimensional structure which is a mirror image of a helix structure, therefore, complexes with insulin into a stereocomplex. In previous reports we demonstrated the formation of such stereocomplex. This study presents the safety evolution of a stereocomplex composed of the water soluble diblock copolymer of D-polylactic acid-co-polyetheylene glycol (DPLA-PEG) and Insulin, following subcutaneous administration of 17 mg sterecomoplex/mouse to Akita<jats:sup>−/+ins2</jats:sup> mice. The mice were monitored for blood glucose levels and weight along the experiment, while growth necropsy and histopathology examination were done post sacrificing. Results demonstrated normal body weight gain with no pathological finding of an internal organ and no inflammatory signs at the injection site except of minimal macrophages, after 16 weeks following polymer administration. Hence, Stereocomplex of D-PLA-PEG/insulin is considered biocompatible with no adversity.","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2024-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140585959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fabrication and evaluation of cross-linked nanogels of Dexibuprofen","authors":"Azka Ikram, Ikrima Khalid, Ikram Ullah Khan, Kashif Barkat, Waqas Ahmad, Haroon Khaild Syed, Ayesha Jamshed","doi":"10.1177/08839115231223962","DOIUrl":"https://doi.org/10.1177/08839115231223962","url":null,"abstract":"The objective of this study was to design and develop an Agarose-based polymeric nanogel network system for solubility enhancement of a lipophilic drug, Dexibuprofen. Polymeric nanogels were synthesized through free radical polymerization where Agarose was cross-linked with 2-Acrylamido-2-methylpropane sulfonic acid (AMPS) in the presence of ammonium persulfate (APS) as an initiator and N, N’-Methylenebisacrylamide (MBA) as crosslinking agent. The resulting polymeric nanogels underwent a comprehensive characterization process including Fourier transform infrared (FTIR), particle size analysis, scanning electron microscopy (SEM), differential scanning calorimetry (DSC), thermal gravimetric analysis (TGA), powder X-ray diffraction (PXRD), and swelling studies to confirm the preparation of a stable polymeric nanogel system. FTIR spectral findings revealed that Agarose was chemically cross-linked with AMPS and confirmed the successful insertion of AMPS chains on the Agarose backbone. Particle size analysis revealed a diameter of 168 nm with a zeta potential of −9.91 mV, providing assurance of a stable polymeric nanogel system. SEM images depicted a highly porous surface. DSC and TGA results showed a more thermally stable network system than individual ingredients. Swelling studies revealed an increased swelling ratio of polymeric nanogels at phosphate buffer of pH 6.8 than acidic buffer of pH 1.2. Dexibuprofen was efficiently loaded into a polymeric nanogel system with a high entrapment efficiency of up to 80%. The solubility of the drug was enhanced when introduced to a polymeric nanogel formulation when compared to pure drug. The system reproducibility was evaluated through in vitro drug release and kinetic modeling of drug release. Toxicity studies confirmed the formulation’s effectiveness, showcasing the developed polymeric nanogels as a promising option for delivering lipophilic drugs, with outstanding physicochemical properties, improved solubility, and minimal oral toxicity.","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2024-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140172320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Digital light processing-based bioprinting of microtissue hydrogel arrays using dextran-induced aqueous emulsion ink","authors":"Yue Xu, Xueyuan Liao, Zhengbi Liu, Lin Zhang","doi":"10.1177/08839115241237327","DOIUrl":"https://doi.org/10.1177/08839115241237327","url":null,"abstract":"As a microscale three-dimensional bionic model, a microtissue array is a promising method for disease modeling and drug screening. Digital light processing (DLP) 3D bio-printing technology with the capabilities of excellent printing speed and resolution shows great potential for fabricating microtissue arrays. However, the outcomes of the microtissue arrays using DLP are limited by the shortage of biomaterials. In this work, we prepare hydrogel microtissues by combining DLP printing technology with biphasic bio-ink consisting of gelatin methacrylate (GelMA) and dextran. GelMA/ dextran is mixed to form bio-inks with different volume ratios, which can achieve high-precision printing of various patterns. Due to the presence of dextran, the bio-inks form the internal porous structure of the hydrogel in the printed patterns. The pore size is related to the proportion of dextran in bio-inks, and the pore size of hydrogel becomes smaller with the increase of dextran ratio. In addition, the adipose-derived stem cells (ADSCs) cells encapsulated in the porous hydrogels can maintain a high survival rate of 98.01% after 3 days of culture, and the larger pore size in the hydrogels promotes cell migration. Combining the DLP printing system with the porous hydrogel allows multiple micro-size geometric pattern hydrogels to be printed simultaneously to form microtissue arrays. In this study, a new bio-ink suitable for microtissue arrays is proposed, and a simple, accurate, and high-throughput biological manufacturing method for microtissue arrays is developed, thus providing a valuable tool for drug screening and tissue engineering.","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2024-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140172306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hyperbranched polyglycidol grafted with rosin by a natural catalyst under ultrasound: Synthesis, characterization and antimicrobial activity","authors":"Amina Mostefai, Mahmoud Belalia, Amine Harrane, Djahira Hamed, Mohammed Amin Bezzekhami, Louiza Belkacemi","doi":"10.1177/08839115241237258","DOIUrl":"https://doi.org/10.1177/08839115241237258","url":null,"abstract":"This study aimed to apply a novel green polymerization process to produce a new copolymer with potential antimicrobial activity for industrial application. The glycidol was polymerized into hyperbranched polyglycidol (HPG) polymer which in turn has been esterified with rosin to produce hyperbranched copoly(glycidol-Rosin) (HPGR), a new co-polymer. The process used Maghnite-H+, a montmorillonite silicate sheet clay, as an eco-catalyst and ultrasound was applied to enhance the interaction during polymerization. Different catalyst amounts were tested to assess their effects on the polymerization process. Fourier Transform Infrared Spectroscopy (FT-IR), Differential Scanning Calorimetry (DSC) and Thermogravimetric Analysis (TGA) were used to analyze the polymer products. The antimicrobial activity of HPGR was assessed against six human standard microbial strains using the agar disc diffusion method. The catalyst percentage of 10% resulted in the best yield for HPGR (35%). Thermogravimetric Analysis (TGA) revealed a best thermal stability of HPGR compared to that of HPG. The HPGR co-polymer displayed the best antibacterial activity against Klebsiella pneumonia, Bacillus cereus, Staphylococcus aureus, and Escherichia coli, producing inhibition zones of 12.33 ± 0.57, 11.00 ± 1.00, 10.66 ± 1.15, and 10.33 ± 1.15 mm, respectively. The hyperbranched copoly(glycidol-Rosin), an eco-catalyst-synthesized co-polymer, displayed interesting physical and antimicrobial properties for industrial application.","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2024-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140105841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Drug-eluting nanofibrous wound dressings composed of polycaprolactone and gelatin augment diabetic wounds healing via reducing oxidative stress and inflammation: An in vitro and in vivo study","authors":"Shungui Liu, Ziqiu Chen","doi":"10.1177/08839115241230969","DOIUrl":"https://doi.org/10.1177/08839115241230969","url":null,"abstract":"Diabetes is a prevalent medical condition that often leads to complications, including diabetic wounds that can have serious consequences such as infections, amputations, and death. In the current research, lodoxamide was loaded into electrospun polycaprolacton/gelatin scaffolds In order to improve wound healing in a rat model. Our developed dressings were characterized in vitro using various methods and then applied on a rat model of excisional wound. Study showed that the dressings were not toxic and promoted migration potential of skin-derived fibroblast cells. Wound healing study showed that lodoxamide-loaded dressings had markedly higher healing potential than other scaffolds and augmented collagen deposition and epithelialization. Gene expression studies showed that TNF-α, glutathione peroxidase, and superoxide dismutase genes were significantly downregulated by lodoxamide-loaded scaffolds.","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2024-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140045672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The enduring impact of a visionary scientist","authors":"Hamid Omidian","doi":"10.1177/08839115241237217","DOIUrl":"https://doi.org/10.1177/08839115241237217","url":null,"abstract":"This study chronicles the seminal contributions of Dr. Raphael M. Ottenbrite, the late Founding Editor of the Journal of Bioactive and Compatible Polymers, to polymer science from the 1970s through 2016. Beginning with an exploration of the biological impacts of anionic polymers, Dr. Ottenbrite’s research evolved to include their applications in immunology and cancer therapy. The 1980s marked a pivotal shift toward the synthesis of polymers geared specifically toward biomedical uses. In the 1990s, his work advanced to include sophisticated synthesis methods and surface modification techniques. The 2000s were characterized by a focus on nanotechnology and the innovation of drug delivery systems. His final research phase, from 2011 to 2016, concentrated on the development of biodegradable materials and advanced drug delivery systems. Dr. Ottenbrite’s work was a unique blend of polymer chemistry, materials science, and biomedicine, laying a vital groundwork for future interdisciplinary research.","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2024-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140033415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrospun silk fibroin/SIS-dECM hierarchical scaffolds for cell growth","authors":"Lusi Chen, Yahao Ma, Longyou Xiao, Pengfei Xie, Jianghui Liu, Wanmei Li, Xiaoying Wang, Liumin He","doi":"10.1177/08839115241233344","DOIUrl":"https://doi.org/10.1177/08839115241233344","url":null,"abstract":"Biomaterials play an important role in biomedical applications serving as scaffolds in tissue engineering and substrates for cell growth by providing structural supports. Signaling cues for cellular events can be also presented, including cell viability, adhesion, spreading, and function developments. In this study, we fabricated a hierarchical nanofibrous scaffold through electrospinning silk fibroin (SF) and decellularized extracellular matrix of the small intestine submucosa (SIS-dECM). The addition of SIS-dECM improved the hydrophilic features of the resultant composite nanofibrous substrates as compared to SF nanofibrous substrates. The biocompatibility of the nanofibrous scaffolds was thoroughly investigated by culturing meningeal fibroblasts on SF and SF/SIS-dECM nanofibrous substrates. Cell viability, proliferation, focal adhesion, and quantitative measurements of cell adhesion-related gene expression levels revealed that the addition of SIS-dECM favored the adhesion and spreading of fibroblast cells. Therefore, the SF/SIS-dECM nanofibers provided a suitable substrate for cell growth and the present study suggested that the nanofibrous substrates hold high potential for bioapplications.","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2024-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139952131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}