Journal of Applied Biomaterials & Functional Materials最新文献

{"title":"Molecular modulation of cell migration via chitosan-based hydrogels: Toward smart biomaterial for wound regeneration.","authors":"Atefe Hosseinkhani, Reyhaneh Molaei, Ali Roosta, Nazanin Mansouri Ghader Abad, Mostafa Saberian","doi":"10.1177/22808000261431513","DOIUrl":"https://doi.org/10.1177/22808000261431513","url":null,"abstract":"<p><p>Chitosan-derived hydrogels offer a highly tunable scaffold for orchestrating the multifaceted process of wound repair, yet their full potential hinges on an integrated understanding of biochemical and biophysical cues. Here, we provide a concise yet comprehensive analysis of design parameters that govern cell-matrix interactions, focusing on polymer backbone modification, crosslinking density, and incorporation of bioactive ligands. We demonstrate how precise control of viscoelastic properties viscoelastic properties, including stiffness and stress relaxation, achieved through reversible covalent bonds and supramolecular assemblies regulates YAP/TAZ nuclear localization and downstream PI3K/Akt and MAPK/ERK signaling to differentially promote coordinated keratinocyte migration, fibroblast proliferation, and endothelial angiogenesis. Functionalization with RGD peptides and heparin-binding motifs further amplifies receptor-mediated adhesion and growth factor sequestration, creating a pro-regenerative microenvironment. We critically appraise the kinetic profiles of growth factor release and immunomodulatory payloads, underscoring the necessity of spatiotemporal precision in smart hydrogel systems and summarize preclinical and clinical evidence demonstrating accelerated wound closure in diabetic and burn models. Finally, we identify pivotal challenges including the quantitative decoupling of mechanical versus molecular influences in vivo, long-term biocompatibility of modified chitosan derivatives, and translation of high-throughput screening data into clinical contexts and propose a roadmap leveraging single-cell transcriptomics and advanced imaging, and AI-driven modeling to overcome translational challenges. This synthesis delivers actionable guidelines for the development of next-generation, personalized wound therapies.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"24 ","pages":"22808000261431513"},"PeriodicalIF":3.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147609020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation and properties of polylactic acid/chitosan composite nanofiber hydrogel for repairing tracheoesophageal fistula.","authors":"Zhikui Luo, Qiang Cao, Leilin Zhu, Mengting Tan, Zhichao Xiao, Junai Zhu, Xinyu Liu, Fusen Peng, Ying Zhang","doi":"10.1177/22808000251412527","DOIUrl":"https://doi.org/10.1177/22808000251412527","url":null,"abstract":"<p><p>In the present study, polylactic acid (PLA) was incorporated to ameliorate the rheological and mechanical properties of chitosan (CS)-based hydrogels. A novel injectable chitosan/polylactic acid (CS/PLA) nanofiber composite hydrogel, fabricated via the electrospinning technique, was developed for the sealing and repair of fistulas, thereby functioning as an innovative biomaterial for the treatment of tracheoesophageal fistula (TEF). Experimental data demonstrated that the CS/PLA composite nanofiber hydrogel exhibits superior mechanical properties, favorable rheological behavior, and prominent antimicrobial activity. At the optimal ratio, the compressive strength and tensile strength of CS/PLA composite nanofiber hydrogel is 43.7 MPa and 1.38 MPa, and the degradation rate is 68.9% after 10 days. Meanwhile, the antibacterial rates against <i>E. coli</i> and <i>S. aureus</i> CS/PLA composite nanofiber hydrogel reached 82.6% and 76.3%, respectively. In vitro experiments were performed to assess the biocompatibility and cell proliferation capacity of the composite hydrogel. The results revealed that the CS/PLA composite nanofiber hydrogel can effectively facilitate the proliferation and migration of target cells, while simultaneously exhibiting favorable biocompatibility. These findings indicate that the CS/PLA composite nanofiber hydrogel possesses considerable application potential in TEF repair, thereby offering an innovative and efficient biomaterial option for clinical intervention of TEF.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"24 ","pages":"22808000251412527"},"PeriodicalIF":3.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146010377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative study on the biodegradation behavior of octacalcium phosphate and hydroxyapatite coated WE43 magnesium alloys.","authors":"Le Van Hai, Do Nhu Ngoc, Pham Mai Khanh, Nguyen Viet Nam","doi":"10.1177/22808000261431918","DOIUrl":"https://doi.org/10.1177/22808000261431918","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to compare the degradation behavior and protection mechanisms of biodegradable WE43 alloys coated with two different calcium phosphate (Ca-P) phases-octacalcium phosphate (OCP) and hydroxyapatite (HA)-to identify an effective surface modification strategy for biodegradable material applications.</p><p><strong>Methods: </strong>OCP and HA coatings were deposited on WE43 substrates and characterized using X-ray diffraction (XRD) and scanning electron microscopy (SEM). The degradation behavior was evaluated through immersion tests in Hanks' balanced salt solution (HBSS) by monitoring surface morphology, magnesium ion release, and pH variation over time.</p><p><strong>Results: </strong>XRD confirmed the successful formation of OCP and HA phases, while SEM revealed distinct morphologies: porous, plate-like structures for OCP and dense, compact layers for HA. Immersion testing demonstrated that both coatings reduced magnesium ion release and stabilized the solution pH compared with uncoated WE43. The HA coating exhibited superior corrosion resistance, maintaining the lowest Mg²⁺ release and most stable pH, attributed to its high crystallinity, compact structure, and self-healing apatite reprecipitation during immersion.</p><p><strong>Conclusion: </strong>Both Ca-P coatings improved the corrosion resistance of WE43 alloy; however, the HA layer provided markedly enhanced and more stable protection. The HA-coated WE43 alloy shows great potential for biodegradable implant applications requiring controlled degradation and sustained biocompatibility.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"24 ","pages":"22808000261431918"},"PeriodicalIF":3.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147468080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Artificial bile duct made of GelMA-SA hybrid hydrogel for bile duct repair.","authors":"Taotao Liu, Xingguang Zhang, Nan Li, Wenhao Ma, Wei Xu, Huijuan Fan, Shihai Xia, Runli Ji","doi":"10.1177/22808000261433066","DOIUrl":"https://doi.org/10.1177/22808000261433066","url":null,"abstract":"<p><p>The management of critical-sized bile duct defects remains a significant clinical challenge, as current options like autologous grafts and synthetic stents provide mechanical support but lack the bioactivity needed for functional regeneration. To address this, we developed a novel tissue-engineered artificial bile duct using a photocrosslinkable gelatin methacryloyl-sodium alginate (GelMA-SA) hybrid hydrogel. This biomimetic artificial bile duct synergizes the superior cell-adhesion of GelMA with the mechanical robustness of SA. A systematically optimized GelMA-SA formulation demonstrated ideal physicochemical properties, including suitable mechanical strength, controlled degradation kinetics, and a conducive microarchitecture for cell growth. Comprehensive in vitro studies confirmed the excellent biocompatibility of artificial bile duct, promoting the proliferation of biliary epithelial cells. Our findings establish this GelMA-SA hybrid hydrogel-based artificial bile duct as a promising repair solution. It effectively bridges the gap between structural support and biological functionality, offering a versatile platform technology for biliary reconstruction and tubular tissue engineering.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"24 ","pages":"22808000261433066"},"PeriodicalIF":3.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147512169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Calcium carbonate polymorphs enhance coagulation in citrated blood via platelet concentration and calcium release.","authors":"Roni M Hendler, Orly E Weiss, Bhuwan Bhaskar, Akansha Kothidar, Liat Hammer, Romi Feigelman, Danny Baranes","doi":"10.1177/22808000251414598","DOIUrl":"https://doi.org/10.1177/22808000251414598","url":null,"abstract":"<p><p>Citrate, widely used as an anticoagulant in transfusion and extracorporeal therapies, disrupts calcium-dependent coagulation by chelating ionized calcium, resulting in hypocalcemia and severe coagulopathy. This interference elevates bleeding risks and may trigger hemorrhagic shock. Current treatments for local bleedings in the context of citrate-induced coagulopathy include topical hemostats that either provide a physical barrier to the bleeding or activate platelets and the coagulation cascade. This study investigates the potential of calcium carbonate (CaCO₃) to counteract the citrate coagulopathic effect by promoting coagulation through calcium ion release and platelet aggregation. In vitro assays demonstrated that various CaCO₃ polymorphs significantly enhance coagulation in citrated blood. Both coral-derived aragonite and synthetic calcite reduced coagulation time, with calcite particles achieving up to a 2-fold reduction. Prothrombin and Partial Thromboplastin Times decreased by 13% and 24%, respectively, with calcite treatment. Moreover, calcite reduced circulating platelet counts by 13% while directly binding platelets, indicating effective recruitment, and raised free calcium levels in plasma by 2.6-fold compared to controls. These dual effects-calcium elevation and platelet concentration-suggest that CaCO₃ is a promising hemostatic agent for addressing bleeding in citrate-induced coagulopathy, offering innovative solutions for transfusion medicine and critical care.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"24 ","pages":"22808000251414598"},"PeriodicalIF":3.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146226863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Incorporation of MXene into brushite cement: Effects on mechanical, physical, and biological properties.","authors":"Bilal Zaman Babar, Muhammad Adnan Khan, Saad Liaqat, Faiza Sharif, Saeed Ur Rahman, Sandleen Feroz, Nawshad Muhammad","doi":"10.1177/22808000261426769","DOIUrl":"https://doi.org/10.1177/22808000261426769","url":null,"abstract":"<p><strong>Objective: </strong>Brushite cement (BrC) has been extensively utilized in hard tissue engineering implementations due to their osteoconductive and bioresorbable nature. The key limitations associated with the clinical application of brushite cement are complex handling procedures, short working time, and inadequate mechanical strength. This study investigates the development of MXene-incorporated brushite cement with improved properties for various clinical applications. The objective was to evaluate the influence of MXene concentration on the mechanical, physical and biological properties of BrC.</p><p><strong>Method: </strong>The incorporated MXene at 1% and 2% concentrations were evaluated for characterization by SEM, EDX, XRD, FTIR, mechanical strength by UTM, physical properties by degradation and setting time, and biologically by DDT, DCT, cytotoxicity and gene expression.</p><p><strong>Results: </strong>MXene inclusion increased the crystallinity of BrC, mechanical strength (SBS, CS and BFS), significantly (<i>p</i> < 0.05) particularly at 2 wt.%. The prolonged setting times, enhanced antibacterial property (ZOI 20.5 mm in DDT; 1.20 OD prior and 0.05 OD after the test in DCT), and acceptable cytocompatibility around 75% cell viability. Significant upregulation of RUNX2, ALP and OCN in the 2% group.</p><p><strong>Conclusion: </strong>Inclusion of MXene into brushite cement generated a composite biomaterial with improved mechanical strength, controlled degradation, strengthened antibacterial activity, while maintained biocompatibility. Exhibits potential for clinical applications requiring enhanced regenerative ability and antibacterial performance.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"24 ","pages":"22808000261426769"},"PeriodicalIF":3.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147690439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation and characterization of PCL@β-TCP composite antibacterial scaffolds by additive manufacturing.","authors":"Chen Zhang, Yi Qi, Binying Dai, Runyi Lin, Jinchao You, Yaomei Wang, Shunxue Xing, Yuansong Ye, De-Jing Li","doi":"10.1177/22808000261421820","DOIUrl":"10.1177/22808000261421820","url":null,"abstract":"<p><p>Poly(ε-caprolactone) (PCL) and β-tricalcium phosphate (β-TCP) are promising materials for bone tissue engineering due to their complementary biodegradability and osteoconductivity. However, their individual limitations hinder clinical application. In this study, PCL@β-TCP composite scaffolds containing 5%, 10%, and 20% β-TCP were fabricated via fused deposition modeling (FDM). The effects of β-TCP content on the physicochemical, mechanical, and antibacterial properties of the scaffolds were systematically investigated. Results showed that increasing β-TCP content enhanced hydrophilicity (contact angle reduced from 89.1° to 66.9°), accelerated biodegradation (higher weight loss after 103 days in PBS), and improved antibacterial activity, as evidenced by larger inhibition zones against <i>E. coli</i> and <i>S. aureus</i>. These findings demonstrate that β-TCP incorporation effectively enhances the bioactivity and antibacterial performance of PCL scaffolds, highlighting their potential for bone tissue engineering applications, particularly in non-load-bearing contexts.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"24 ","pages":"22808000261421820"},"PeriodicalIF":3.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147306236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Human mesenchymal stem cell expansion on laminin-521 in serum-free and xeno-free culture conditions.","authors":"Halima Albalushi, Mohadese Boorojerdi, Elias Said, Halima Al Shehhi, Nihal Al Riyami, Mohammed Al Rawahi, Murtadha Al Khabori","doi":"10.1177/22808000251332110","DOIUrl":"https://doi.org/10.1177/22808000251332110","url":null,"abstract":"<p><p>Laminin-521 (LN521) is a crucial adhesion protein found in natural stem cell niches and plays an important role in maintaining human pluripotent stem cell (PSC) properties. This study aimed to investigate the effects of LN521 on human umbilical cord-derived mesenchymal stem cell (UC-MSC) characteristics in Serum-free and Xeno-free culture conditions as a step toward clinical application. In our experiment isolated UC-MSC via explant method were expanded as a homogeneous monolayer and morphologically, presented typical MSC-like morphology (spindle-shaped) from passage three to six when cultured on either LN521 or CELLstart™. Almost, 90% confluency was reached after 4 days of culture with an EI of approximately 11.2 with no statistically significant differences on LN521 and CELLstart™ in all six passages. Phenotypic characterization of UC-MSC cultured on LN521 or CELLstart™ using flow cytometry, along with the expression of the same biomarkers in gene level analyzed by quantitative reversed transcription revealed identical CD73, CD90, CD105, CD34, CD45, CD19, CD14, and HLA-DR expression pattern at passages three and six in both LN521 and CELLstart™. Moreover, UC-MSC cultured in the presence of LN521 and CELLstart™ showed the same adipogenesis, chondrogenesis and osteogenesis differentiation potential, and normal chromosome structure highlighting genetic stability. Ultimately, LN521 is comparable to CELLstart™ in supporting UC-MSC expansion and maintaining their characteristics in serum-free and xeno-free culture conditions.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"23 ","pages":"22808000251332110"},"PeriodicalIF":3.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144002088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioactive oligopeptides and the application in skin regeneration and rejuvenation.","authors":"Qiulin He, Youguo Liao, Yaru Wu, Huahui Zhang, Xiaohui Long, Yuxiang Zhang","doi":"10.1177/22808000251330974","DOIUrl":"https://doi.org/10.1177/22808000251330974","url":null,"abstract":"<p><p>Oligopeptides, composed of 2-10 amino acid residues, are protein fragments with unique structural characteristics, including small molecular size, high biocompatibility, and modifiable functional groups. These features endow oligopeptides with excellent permeability, safety, and versatile biological activities, making them widely applicable in disease treatment, drug delivery, and skincare. In particular, oligopeptides have emerged as advanced ingredients in skincare, offering anti-aging, anti-wrinkle, and whitening effects by regulating key biological processes such as collagen synthesis, antioxidant defense, and melanin production. This review comprehensively discusses the structural properties, functional mechanisms, and diverse applications of oligopeptides and their derivatives, highlighting their potential in skin regeneration, rejuvenation, and anti-aging medicine. By providing insights into the latest advancements, this review aims to serve as a valuable reference for future research and development in oligopeptide-based therapeutics and skincare innovations.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"23 ","pages":"22808000251330974"},"PeriodicalIF":3.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144000586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of Ion Leachable Resin Composites: Time-Dependent Water Sorption, Solubility and Hygroscopic Expansion.","authors":"Abdullah Alshehri, Ahmed A Almokhatieb, Mohammed Mustafa, Khaled Abid Althaqafi, Waseem Waleed Radwan, Mohammad Khursheed Alam","doi":"10.1177/22808000251348969","DOIUrl":"https://doi.org/10.1177/22808000251348969","url":null,"abstract":"<p><strong>Aim: </strong>This study aimed to assess the time-dependent water sorption, solubility and hygroscopic expansion of experimental resin composites modified with three different types of ion-leachable glasses (ILGs): 45S5 Bioglass (BG), Fluoride-containing glass (F9) and Experimental fluoride-phosphate glass (F9X), incorporated in varying weight percentages (5%, 10% and 15%).</p><p><strong>Materials and methods: </strong>A 50:50 Bis-GMA/TEGDMA-based resin matrix was loaded with each ILG type in 5, 10 and 15 wt% and compared against a control without filler. Disc-shaped specimens (<i>n</i> = 3 per group; total = 39) were fabricated using a stainless-steel mould and cured using an LED light-curing unit (1200 mW/cm², 20 s per side). The water sorption and solubility were evaluated using a modified ISO 4049 protocol over a 12-week immersion period in distilled water at 37°C, followed by an 8-week desorption phase. Hygroscopic expansion was evaluated through volume change using a digital micrometre. Data were statistically analysed using one-way ANOVA and Tukey's post-hoc test.</p><p><strong>Results: </strong>All ILG-containing composites showed significantly increased water sorption compared to the control, with the BG-15 group demonstrating the highest sorption (3.37% ± 0.09) and expansion. Solubility increased with ILG concentration, especially in the BG and F9X groups. Hygroscopic expansion correlated positively with water uptake. No significant changes were observed in specimen mass after desorption in low filler groups.</p><p><strong>Conclusion: </strong>The incorporation of ILGs into resin composites significantly altered their water uptake and dimensional stability. While these effects could compromise long-term mechanical properties, the resulting hygroscopic expansion may aid in reducing microgaps and secondary caries at restoration margins. Veneering ILG-containing composites with conventional materials is recommended to limit degradation. These findings contribute novel insights into time-dependent dimensional behaviour of bioactive composites.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"23 ","pages":"22808000251348969"},"PeriodicalIF":3.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144567464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}