Journal of Applied Biomaterials & Functional Materials最新文献

{"title":"Investigating the early osteogenic potential of strontium doped titanium alloy surface.","authors":"Ali Alenezi, Mohamed Ahmed Alkhodary","doi":"10.1177/22808000251328992","DOIUrl":"https://doi.org/10.1177/22808000251328992","url":null,"abstract":"<p><p>The current study was conducted to test the early osteogenic potential of strontium (Sr) doped titanium-aluminum-vanadium (Ti-6Al-4V) alloy using an in vitro cell culture experiment. Thirty Ti-6Al-4V alloy sheets were sandblasted and etched with large grit acid. Of these, 10 alloy sheets represented group I, 10 sheets doped with Sr using a hydrothermal process represented group II, and 10 sheets also coated with Sr-supplemented hydroxyapatite using a hydrothermal process represented group III. The surfaces of the three groups were characterized by scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), and X-ray diffraction (XRD). The three groups were also compared in terms of their water contact angle, protein adsorption, and Sr ion release profile. Further, bone marrow mesenchymal cells (BMSCs) obtained from the femora of 10 Sprague Dawley rats were used for the in vitro cell culture experiment, and the viability of the cultured cells was evaluated using the MTT assay and confocal microscopy; in addition, their osteogenic potential was assessed using alkaline phosphatase ALP activity. For statistical analysis, data were analyzed using one-way analysis of variance (ANOVA; IBM SPSS statistics 23), and <i>p</i> < 0.05 was considered statistically significant. SEM images revealed that the three groups had surface roughness, and EDS and XRD revealed the success of incorporating Sr to their surfaces. Group III had the best contact angle, protein adsorption, and Sr ion release rate. The cell culture also revealed that the surfaces of the titanium alloy sheets in group III were the most viable and had the best osteogenic potential; however, there was no statistically significant difference between groups I and II. In Conclusion, Sr alone was not able to improve the osteogenic potential of titanium alloy surfaces.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"23 ","pages":"22808000251328992"},"PeriodicalIF":3.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144021745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of pull-out bond strength and microleakage of lithium disilicate onlay with and without immediate dentin sealing.","authors":"Aruna Krishnan, Nishitha Arun, Mohammad Fareed, Nisha Manila, Mohmed Isaqali Karobari","doi":"10.1177/22808000251329112","DOIUrl":"https://doi.org/10.1177/22808000251329112","url":null,"abstract":"<p><strong>Background: </strong>Effective adhesion between dentin and resin materials is essential for the long-term durability of onlays, as the strength and durability of the tooth-restoration complex hinge on reliable adhesion. This study aims to enhance the adhesion of lithium disilicate onlays by employing immediate dentin sealing (IDS) and evaluates its effectiveness by comparing pull-out bond strength and microleakage with and without IDS.</p><p><strong>Methods: </strong>Extracted human premolars were prepared and categorized into two distinct groups: Group A (with IDS) and Group B (without IDS). In Group A, the dentin surfaces were sealed immediately after tooth preparation using an adhesive resin. In Group B, no sealing was performed before bonding the lithium disilicate restorations. Pull-out bond strength was assessed utilizing universal testing machine. Microleakage was evaluated through dye penetration analysis after thermocycling and sectioning of the samples. For statistical interpretation, one-way ANOVA followed by Tukey's post-hoc test was applied.</p><p><strong>Results: </strong>The pull-out bond strength was significantly higher in Group A compared to Group B (<i>p</i> = 0.0001). Microleakage evaluation revealed less dye penetration in Group A, indicating superior marginal integrity with IDS. The high-resolution images provided clearer visualization of the interfaces and the extent of dye penetration.</p><p><strong>Conclusion: </strong>Immediate dentin sealing significantly enhances the pull-out bond strength of lithium disilicate onlay and reduces microleakage. These findings suggest that IDS is a beneficial step in the bonding protocol for lithium disilicate restorations, potentially improving the longevity and performance of the restorations.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"23 ","pages":"22808000251329112"},"PeriodicalIF":3.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144019295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cranioplasty infection in porous hydroxyapatite: Potential antibacterial properties.","authors":"Ismail Zaed, Corrado Iaccarino, Francesca Faedo, Laura Grillini, Elisabetta Galassi, Alessandro Dotti, Angelo Nataloni, Francesca Carolina Mannella, Andrea Cardia","doi":"10.1177/22808000241311389","DOIUrl":"10.1177/22808000241311389","url":null,"abstract":"<p><strong>Introduction: </strong>Intensive research is dedicated to the development of novel biomaterials and medical devices to be used as grafts in reconstructive surgery, with the purpose of enhancing their therapeutic effectiveness, safety, and durability. A variety of biomaterials, from autologous bone to polymethylmetacrylate, polyether ether ketone, titanium, and calcium-based ceramics are used in cranioplasty. Porous hydroxyapatite (PHA) is reported as a possible material for bone reconstruction, with good signs of biocompatibility, osteoconductive and osteointegrative properties. In the present paper we studied the possible antibacterial properties of PHA in a laboratory test in order to provide a possible overview of the occurrence of post-operative infections in PHA cranioplasty.</p><p><strong>Method: </strong>The test method has been designed to evaluate the potential antimicrobial activity of specimens under dynamic contact conditions to overcome difficulties in ensuring contact of inoculum to the specimen surface. The test was conducted using <i>Staphylococcus aureus</i> ATCC6538 as a bacterial strain.</p><p><strong>Results: </strong>Two experimental sets were performed to evaluate the antimicrobial properties of the specimens against two different <i>Staphylococcus aureus</i> concentrations. The first preliminary test (a) verified the antibacterial property at 0, 1, 2, and 4 h of contact time; the second confirmatory test (b) was repeated to verify the antibacterial property at 0, 4, 8 h. In the first experiment, after the first hour of contact, the bacterial inoculum was reduced by 7.96% compared to \"inoculum only,\" which increased to 26.11% at the second hour, and up to 52.33% after 4 h. In the second experiment, the confirmation test showed that bacterial growth reaches maximum inhibition after 4 h of contact. At 4 h, there was a higher bacterial reduction of 72.93%, which decreased at 8 h (36.45%).</p><p><strong>Conclusion: </strong>Analyzing the growth trend of viable microorganisms under Dynamic Contact Conditions it can be seen that PHA cranioplasty appears to inhibit exponential growth by inducing bacterial stasis in the early hours of contact, reaching a maximum reduction within 4 h, in this adopted experimental condition.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"23 ","pages":"22808000241311389"},"PeriodicalIF":3.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143038852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Less cytotoxic phthalocyanine derivative promotes in vitro wound healing compared to chlorhexidine.","authors":"Caique Andrade Santos, Adriano de Souza Pessoa, Fabiano Vieira Vilhena, Rodrigo Cardoso de Oliveira, Paulo Sérgio da Silva Santos, Carla Andreotti Damante, Mariana Schutzer Ragghianti Zangrando","doi":"10.1177/22808000251314630","DOIUrl":"10.1177/22808000251314630","url":null,"abstract":"<p><p>The use of adjunct chemical substances in the early postoperative period of periodontal surgical procedures is recommended due to the potential risk of trauma in the operated area. Chlorhexidine digluconate mouthwash is widely used but can cause adverse effects. Phthalocyanine derivatives are being studied as an alternative, demonstrating good antimicrobial activity, especially in the self-activated form, which does not require additional light or chemicals. The objective of this study is to compare the cytotoxicity of different concentrations of a phthalocyanine (PHY) with chlorhexidine (CHX) and assess their influence on fibroblast cell migration. Different concentrations of CHX and PHY (0.0075%-0.12%) were evaluated using NIH 3T3 fibroblasts. Cell viability was assessed by the MTT and crystal violet (CV) assay; CHX and PHY (0.0075% and 0.12%) were also evaluated by in vitro wound healing assay. PHY was less cytotoxic compared to CHX, based on cell viability assays. PHY did not interfere with experimental healing, allowing cell migration similar to the positive control with both concentrations (PHY 0.0075% and 0.12%) and only 0.0075% CHX allowed cell migration. In a comparative analysis, PHY showed less cytotoxicity than CHX and PHY concentrations of 0.0075% and 0.015% was non-toxic even after 48 h of contact with the cells. This in vitro evaluation demonstrated that PHY was less cytotoxic to NIH 3T3 fibroblasts compared to CHX. Furthermore, the different concentrations of PHY did not interfere negatively in the healing of experimental wounds.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"23 ","pages":"22808000251314630"},"PeriodicalIF":3.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143065809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-layered electrospun grafts for surgical repair: Biomimicking physiological ascending aortic compliance.","authors":"Julia Krammer, Maximilian Pichlmaier, Jan Stana, Christian Hagl, Sven Peterss, Maximilian Grab, Linda Grefen","doi":"10.1177/22808000251316728","DOIUrl":"10.1177/22808000251316728","url":null,"abstract":"<p><p>Commercially available Dacron (woven polyester) grafts are used for routine open surgical repair of thoracic aortic aneurysms. Despite durable and biocompatible, these grafts do not reproduce the natural mechanical properties of the aorta. Therefore, the aim of this project was to develop an innovative graft that additionally exhibits physiological aortic compliance. To achieve this result, multi-layered tubular aortic grafts were created by electrospinning of a thermoplastic polyurethane. To reduce permeability, a gelatin-coating was added. Three groups (G_1-3; <i>n</i> = 5) with varying layer designs were evaluated regarding the main mechanical properties of vascular grafts such as suture retention strength, permeability and static and dynamic compliance. G₃, which combined electrospinning with a stable silicone-coated inlay was chosen for the fabrication of medical grade thermoplastic polyurethane grafts (G_m; <i>n</i> = 6). Dynamic compliance values of 19.68 ± 11.5%/100 mmHg (50-90 mmHg), 15.18 ± 8.7%/100 mmHg (80-120 mmHg) and 14.56 ± 7.4%/100 mmHg (110-150 mmHg) were achieved. The compliance was higher than for Dacron and ePTFE grafts and comparable to the normal sized ascending aorta of around 16%/100 mmHg in a healthy human and porcine aortic compliance of 14.3%/100 mmHg. Static compliance was successfully tested up to 350 mmHg. No significant changes in graft diameter or delaminations of the graft layers were detected after compliance testing. Therefore, by combining electrospinning with a durable inlay, both elasticity and recoverability are obtained, resulting in a promising alternative to the gold-standard in open-surgical treatment of thoracic aortic pathologies.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"23 ","pages":"22808000251316728"},"PeriodicalIF":3.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143374008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Amniotic membrane hydrogel as novel injectable platform in combination with metformin for treatment of sciatic nerve injury.","authors":"Zahra Rabiei Dolatabadi, Mona Saheli, Somayeh Solhjoo, Mehran Hosseini, Elahe Mousanejad, Mandana Jafari, Vahid Sheibani, Seyed Noureddin Nematollahi-Mahani","doi":"10.1177/22808000251322865","DOIUrl":"10.1177/22808000251322865","url":null,"abstract":"<p><p>Peripheral nerve tissue engineering is a field that uses cells, growth factors and biological scaffold material to provide a nutritional and physical support in the repair of nerve injuries. The specific properties of injectable human amniotic membrane-derived hydrogel including growth factors as well as anti-inflammatory and neuroprotective agents make it an ideal tool for nerve tissue repair, and metformin may also aid in nerve regeneration. The aim of this study was to investigate the effects of hydrogel derived from amniotic membrane (AM) along with metformin (MET) administration in the repair of sciatic nerve injury in male rats. We randomly divided 60 male rats into five groups. A control and four sciatic nerve compression groups including model; hydrogel; metformin and mix which received hydrogel and metformin. The recovery rate was assessed by Sciatic Functional Index (SFI), Static Sciatic Index (SSI) and von-frey test. Conduction velocity of the sciatic nerve was measured by Electrophysiological studies, and histological evaluations were performed 14 days after injury. SFI, SSI, latency time, remyelination rate and the expression of NF-200 and S-100β improved in hydrogel group. Response to mechanical stimulus, myelin density, axonal regeneration, and myelin sheath reconstruction improved in the mix group. The gastrocnemius muscle index was significantly reduced in the experimental groups while collagen fibers increased in these groups. These findings suggest that injection of hydrogel derived from decellularized amniotic membrane into the epineurium can be promoted reconstruction of peripheral nerve injury and improved functional nerve recovery. Also, metformin administration can reinforce the therapeutic effect of the hydrogel.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"23 ","pages":"22808000251322865"},"PeriodicalIF":3.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143604785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polymeric hollow fibers for encapsulating <i>Eucalyptus galbie</i> essential oil with decongestant potential and physicochemical study.","authors":"Reyhane Shojaei, Mehdi Ansari, Neda Mohamadi, Fariba Sharififar","doi":"10.1177/22808000251335735","DOIUrl":"https://doi.org/10.1177/22808000251335735","url":null,"abstract":"<p><p><i>Eucalyptus</i> species are known for their decongestant and antimicrobial properties, mainly due to their essential oil. However, significant challenges persist in maintaining the stability and durability of the essential oils' effectiveness. This study examines the use of polymeric hollow fibers for encapsulating <i>Eucalyptus</i> essential oil (EE) to improve its stability and effectiveness in treating nasal congestion. The EE was obtained by hydro-distillation method, and analyzed using gas chromatography-mass spectrometry (GC-MS). A dialysis cartridge containing polysulfone hollow fibers was used to load the EE, and their permeability, morphology, and stability were assessed. Fourier-transform infrared spectroscopy (FT-IR) and headspace sampling with gas chromatography-flame ionization detection (GC-FID) were employed to monitor EE release and the stability of EE-loaded hollow fibers. GC/MS analysis identified 20 major components with 1,8-cineole being the predominant compound at 59.32%. Physicochemical characterization of hollow fibers revealed complete permeability to EE. FT-IR spectra suggested potential interactions between EE and the fibers. Release studies indicated that over 80% of EE was released from the fibers within 180 min. Headspace analysis confirmed the presence and stability of 1,8-cineole in the loaded fibers. The stability test demonstrated no significant changes in the EE-loaded fibers over 6 days. This study indicated that the capillary properties of the EE-loaded hollow fibers facilitate oil loading, and headspace sampling provides a more efficient analysis. The successful and stable release of EE from the fibers, highlights the potential of hollow fibers for controlled drug delivery. However, conducting more accurate experiments can help deduce more logical results.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"23 ","pages":"22808000251335735"},"PeriodicalIF":3.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144101909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of nano-chitosan and <i>Saussurea costus</i> as mouthwash and denture cleanser: In vitro study.","authors":"Sabreen Waleed Ibrahim, Farah Nabeel Mohammed Tahir AlKhayyat, Maha Jamal Abbas","doi":"10.1177/22808000251337545","DOIUrl":"https://doi.org/10.1177/22808000251337545","url":null,"abstract":"<p><strong>Introduction: </strong>Besides mechanical cleaning, mouthwashes, and denture cleansers are typical supplements for oral care. Many chemical mouthwashes and denture cleansers provide positive effects, but finding safe and natural alternatives should be the priority.</p><p><strong>Aim of study: </strong>This study aims to investigate the effectiveness of natural materials as mouthwash and denture cleansers.</p><p><strong>Material and method: </strong>In examining the antimicrobial efficacy of mouthwashes, the minimum inhibitory concentration values were determined for five groups: distilled water (negative control), 0.2% chlorhexidine, chitosan solution, <i>Saussurea costus</i> solution, and chitosan-Saussurea solution. As for denture cleansers, the efficacy was evaluated after immersing polymethylmethacrylate (PMMA) disks in the previous solutions. The cell viability of Human Gingival Fibroblast (hGFs) after treatment with tested solutions was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays. Data was analyzed using ANOVA with Bonferroni post hoc tests.</p><p><strong>Result: </strong>The prepared solutions showed comparable antimicrobial efficacy and cell viability as chlorohexidine. The chitosan-<i>Saussurea costus</i> solution demonstrated a higher antimicrobial effect and better prevention of microorganisms' adherence to PMMA disks and cell viability. It also did not affect the roughness of PMMA in the short term.</p><p><strong>Conclusion: </strong>The new denture cleaning and mouthwash agent, which contains the natural constituents of chitosan and <i>Saussurea costus</i>, demonstrates significant antibacterial effectiveness and serves as a viable eco-friendly alternative to commercial mouthwash, as well as exerting minimal influence on denture roughness.</p><p><strong>Clinical implications: </strong>Chitosan and <i>Saussurea costus</i> have antibacterial and anti-inflammatory properties that can potentially enhance oral health. These materials present a viable path toward the production of safe, efficient, and sustainable oral care products due to their natural and biocompatible substitutes.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"23 ","pages":"22808000251337545"},"PeriodicalIF":3.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144003158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancements in biomaterials and scaffold design for tendon repair and regeneration.","authors":"Ahmad Alhaskawi, Yanzhao Dong, Xiaodi Zou, Weijie Zhou, Sohaib Hasan Abdullah Ezzi, Vishnu Goutham Kota, Mohamed Hasan Abdulla Hasan Abdulla, Sahar Abdalbary, Hui Lu","doi":"10.1177/22808000241310684","DOIUrl":"https://doi.org/10.1177/22808000241310684","url":null,"abstract":"<p><p>Tendon injuries present a significant clinical challenge due to their limited natural healing capacity and the mechanical demands placed on these tissues. This review provides a comprehensive evaluation of the current strategies and advancements in tendon repair and regeneration, focusing on biomaterial innovations and scaffold design. Through a systematic literature search of databases such as PubMed, Scopus, and Web of Science, key studies were analyzed to assess the efficacy of biocompatible materials like hydrogels, synthetic polymers, and fiber-reinforced scaffolds in promoting tendon healing. Emphasis is placed on the role of collagen fiber architecture, including fiber diameter, alignment, and crimping, in restoring the mechanical strength and functional properties of tendons. Additionally, the review highlights emerging techniques such as electrospinning, melt electrowriting, and hybrid textile methods that allow for precise scaffold designs mimicking native tendon structures. Cutting-edge approaches in regenerative medicine, including stem cell therapies, bioelectronic devices, and bioactive molecules, are also explored for their potential to enhance tendon repair. The findings underscore the transformative impact of these technologies on improving tendon biomechanics and functional recovery. Future research directions are outlined, aiming to overcome the current limitations in scaffold mechanical properties and integration at tendon-bone and tendon-muscle junctions. This review contributes to the development of more effective strategies for tendon regeneration, advancing both clinical outcomes and the field of orthopedic tissue engineering.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"23 ","pages":"22808000241310684"},"PeriodicalIF":3.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144150545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Silicate-substituted bovine-derived hydroxyapatite as a bone substitute in regenerative dentistry.","authors":"Jithendra Ratnayake, Niranjan Ramesh, Maree L Gould, Michael R Mucalo, George J Dias","doi":"10.1177/22808000251314302","DOIUrl":"10.1177/22808000251314302","url":null,"abstract":"<p><p>Hydroxyapatite, renowned for its biocompatibility and osteoconductive properties, plays a fundamental role in bone regeneration owing to its resemblance to natural bone mineral, thus offering considerable potential for advancing tissue engineering strategies. In this article, the innovative integration of silicon ions into biogenic (bovine-derived) hydroxyapatite (SiBHA) via a tailored sol-gel process is reported. The resultant SiBHA scaffolds exhibited an interconnected microporous structure with a total porosity of 70% and pore dimensions ranging from 120 to 650 µm. Fourier-transform infrared spectroscopy and X-ray diffraction studies validated the effective incorporation of silicon ions into the BHA lattice, with energy-dispersive X-ray and inductively-coupled plasma mass spectrometry further confirming a Ca/P molar ratio for SiBHA between 1.63 and 1.74. Moreover, SiBHA scaffolds demonstrated commendable chemical and thermal stability. Of note, SiBHA scaffolds were found to display significantly enhanced mechanical properties, including compressive strength and Young's modulus, compared to the control BHA scaffolds. In vitro assessments highlighted the capacity of SiBHA scaffolds to foster cell viability, proliferation, and osteogenic differentiation of Saos-2 cells. Immunohistochemical analysis revealed a significant increase in osteonectin expression, a key bone matrix protein, after 14 days of incubation under osteogenic conditions. These findings highlight the biocompatibility and therapeutic potential of SiBHA scaffolds, suggesting their suitability as biomaterials for dental bone regeneration applications.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"23 ","pages":"22808000251314302"},"PeriodicalIF":3.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143023025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}