Journal of Applied Biomaterials & Functional Materials最新文献

{"title":"Human mesenchymal stem cell expansion on laminin-521 in serum-free and xeno-free culture conditions.","authors":"Halima Albalushi, Mohadese Boorojerdi, Elias Said, Halima Al Shehhi, Nihal Al Riyami, Mohammed Al Rawahi, Murtadha Al Khabori","doi":"10.1177/22808000251332110","DOIUrl":"https://doi.org/10.1177/22808000251332110","url":null,"abstract":"<p><p>Laminin-521 (LN521) is a crucial adhesion protein found in natural stem cell niches and plays an important role in maintaining human pluripotent stem cell (PSC) properties. This study aimed to investigate the effects of LN521 on human umbilical cord-derived mesenchymal stem cell (UC-MSC) characteristics in Serum-free and Xeno-free culture conditions as a step toward clinical application. In our experiment isolated UC-MSC via explant method were expanded as a homogeneous monolayer and morphologically, presented typical MSC-like morphology (spindle-shaped) from passage three to six when cultured on either LN521 or CELLstart™. Almost, 90% confluency was reached after 4 days of culture with an EI of approximately 11.2 with no statistically significant differences on LN521 and CELLstart™ in all six passages. Phenotypic characterization of UC-MSC cultured on LN521 or CELLstart™ using flow cytometry, along with the expression of the same biomarkers in gene level analyzed by quantitative reversed transcription revealed identical CD73, CD90, CD105, CD34, CD45, CD19, CD14, and HLA-DR expression pattern at passages three and six in both LN521 and CELLstart™. Moreover, UC-MSC cultured in the presence of LN521 and CELLstart™ showed the same adipogenesis, chondrogenesis and osteogenesis differentiation potential, and normal chromosome structure highlighting genetic stability. Ultimately, LN521 is comparable to CELLstart™ in supporting UC-MSC expansion and maintaining their characteristics in serum-free and xeno-free culture conditions.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"23 ","pages":"22808000251332110"},"PeriodicalIF":3.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144002088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioactive oligopeptides and the application in skin regeneration and rejuvenation.","authors":"Qiulin He, Youguo Liao, Yaru Wu, Huahui Zhang, Xiaohui Long, Yuxiang Zhang","doi":"10.1177/22808000251330974","DOIUrl":"https://doi.org/10.1177/22808000251330974","url":null,"abstract":"<p><p>Oligopeptides, composed of 2-10 amino acid residues, are protein fragments with unique structural characteristics, including small molecular size, high biocompatibility, and modifiable functional groups. These features endow oligopeptides with excellent permeability, safety, and versatile biological activities, making them widely applicable in disease treatment, drug delivery, and skincare. In particular, oligopeptides have emerged as advanced ingredients in skincare, offering anti-aging, anti-wrinkle, and whitening effects by regulating key biological processes such as collagen synthesis, antioxidant defense, and melanin production. This review comprehensively discusses the structural properties, functional mechanisms, and diverse applications of oligopeptides and their derivatives, highlighting their potential in skin regeneration, rejuvenation, and anti-aging medicine. By providing insights into the latest advancements, this review aims to serve as a valuable reference for future research and development in oligopeptide-based therapeutics and skincare innovations.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"23 ","pages":"22808000251330974"},"PeriodicalIF":3.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144000586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An assessment of synthesis technique for porous nano and micro fibrous wound dressings with natural oil supplements.","authors":"Meltem Kum, Levent Sendogdular, Selda Topcu Sendogdular","doi":"10.1177/22808000251314106","DOIUrl":"10.1177/22808000251314106","url":null,"abstract":"<p><p>For millennia, aloe vera (AV) and eucalyptus oil (EO) have been recognized as natural sources of healing and have been utilized for medicinal purposes in the realm of health. As an attempt to treat pressure sores, AV and eucalyptus oil were added as supplements to biocompatible and biodegradable poly (ethylene oxide) (PEO) polymer to synthesize nano and micro fibrous wound dressings by the electrospinning process. Additive solubility in polymeric matrix is the key parameter to achieve the synthesis of homogeneous fibers with controlled release of therapeutic oils, cure and humidity; therefore, lecithin as herbal (soybean) based emulsifier was used to control additive/polymer solubility. In this study, fibrous dressing in mat form with antioxidant activity was successfully obtained with the addition of natural AV and EO in PEO polymer solutions through electrospinning technique. Subsequently, the synthesized fibers were examined via scanning electron microscopy (SEM), thermogravimetric analysis (TGA), moisture absorption and UV-Vis spectroscopy. SEM imaging demonstrated the formation of randomly-oriented and beadless fibers with size of 0.48 ± 0.23 µm out of PEO/AV/EO/Lecithin blend and also with the addition of lecithin, fiber thicknesses were observed to be increasing. Moisture absorption analysis revealed that the weight of fibrous mat was affected by the humidity of the ambient environment. Relative humidity for 7 days ranged between 32% and 37% and it was observed that lecithin content increased the moisture retention rate by 50%. Uv-Vis results suggested that a more regular performance has been achieved with lecithin being involved in terms of timely manner changes; therefore, the contrast of samples between hours and days became more distinctive. PEO/AV/EO/Lecithin nanofiber also indicated antibacterial ability against <i>Escherichia coli</i> with approximately 18.5 mm diameter of inhibition. This research proves that the potential for developing biocompatible wound dressings with long-lasting moisture to the wound is possible through the use of these natural healing agents made homogeneously distributed through structure by the use of emulsifier.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"23 ","pages":"22808000251314106"},"PeriodicalIF":3.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143604802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Controlled release and antibioticeffect of vancomycin-loaded poly(<i>N</i>-isopropylacrylamide) nanoparticles as a smart drug delivery system.","authors":"Liang Lei, Shanlong Li, Xuefeng Kang, Jian Shang","doi":"10.1177/22808000241291824","DOIUrl":"10.1177/22808000241291824","url":null,"abstract":"<p><p>Numerous studies have addressed the use of vancomycin (VA) to effectively treat bacterial infections. However, VA is known to cause side effects when administered intravenously. Herein, monodisperse poly(<i>N</i>-isopropylacrylamide) (PNIPAAm) hollow nanocapsules were synthesized at the interface of a water-in-oil (W/O) single emulsion via Shirasu porous glass (SPG) membrane emulsification and UV-initiated polymerization. In water solutions, the PNIPAAm nanocapsules were able to encapsulate VA and form a new nanoscale water-soluble drug delivery system, namely, PNIPAAm-VA. In vitro experiments showed that PNIPAAm and PNIPAAm-VA had no cytotoxicity toward human bone marrow mesenchymal stem cells. In addition, the slow hydrolysis of PNIPAAm-VA in vitro led to the progressive release of VA, which was discharged at more than 50% and 80% of its initial concentration within 10 days at 37°C and 40°C, respectively; this subsequently inhibited the growth of methicillin-resistant Staphylococcus aureus bacteria. We believe that our PNIPAAm-VA nanoparticles can potentially be used as an effective injectable for temperature-sensitive materials in vivo to achieve the localized controlled release of drugs as safe and specific therapeutic agents.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"23 ","pages":"22808000241291824"},"PeriodicalIF":3.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143634041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The influence of stainless steel crowns placed on primary teeth on biochemical parameters in GCF: A systematic review.","authors":"Mario Alberto Alarcón-Sánchez, Julieta Sarai Becerra-Ruiz, Ruth Rodríguez-Montaño, Sarah Monserrat Lomelí-Martínez, Luca Fiorillo, Artak Heboyan","doi":"10.1177/22808000251335403","DOIUrl":"https://doi.org/10.1177/22808000251335403","url":null,"abstract":"<p><strong>Objective: </strong>The aim of this systematic review was to investigate, identify, and summarize the existing literature on the levels of proinflammatory cytokines and chemokines present in gingival crevicular fluid (GCF) of primary teeth restored with stainless steel crowns (SSC) versus control teeth.</p><p><strong>Materials and methods: </strong>The systematic review was registered in the Open Science Framework (ID): 10.17605/OSF.IO/39U4D. In addition, it was prepared following the guidelines of the Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA). Five electronic databases were used to identify studies for this systematic review: PubMed, Scopus, ScienceDirect, Web of Science, and Google Scholar, from January 10, 1999, to September 15, 2024. The risk of bias in the included studies was assessed using Joanna Briggs Institute (cross-sectional studies) and the Cochrane Risk of Bias for Randomized Trials (RoB 2.0) in randomized clinical trials.</p><p><strong>Results: </strong>The review includes four studies (two cross-sectional and two randomized clinical trials). A total of 75 children aged 3 to 10 years were studied. GCF samples were taken from 98 upper and lower molars rehabilitated with SSC and control teeth (without SSC). ELISA analyzed all samples. This way, the levels of four proinflammatory cytokines and chemokines, IL-1β, IL-6, MIP-1α, and MIP-1β, were determined. The studies reported significant differences between both study groups: IL-1β: 27.30 versus 23.56 <i>p</i> < 0.05; MIP-1α: 682.55 versus 197.60 <i>p</i> < 0.05; and MIP-1β: 884.35 versus 287.85, <i>p</i> < 0.05.</p><p><strong>Conclusions: </strong>This systematic review provides a comprehensive and current overview of the concentrations of proinflammatory cytokines and chemokines present in GCF, providing new insights into the pathogenesis of gingival inflammation in children with SSC. IL-1β, MIP-1α, and MIP-1β levels increased in the GCF of upper and lower molars rehabilitated with stainless steel crowns compared to control primary teeth.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"23 ","pages":"22808000251335403"},"PeriodicalIF":3.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143966698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regenerative effect of human cell-derived BMP-2 cultured within a collagen bone graft in a rat calvaria defect model.","authors":"Keun-Suh Kim, Euna Pi, Sang-Jun Park, Seungyoon Lee, Inyoung Choi, Taeuk Kim, Hyo-Jung Lee, Yang-Jin Yi","doi":"10.1177/22808000251326799","DOIUrl":"https://doi.org/10.1177/22808000251326799","url":null,"abstract":"<p><p>Bone morphogenetic protein-2 (BMP-2) is a potent osteoinductive factor; however, current clinical applications using <i>Escherichia coli</i>-derived recombinant human BMP-2 are constrained by structural deficiencies and high-dose requirements, which increases the risk of adverse effects. In this study, we aimed to address these limitations by developing a novel approach leveraging genetically engineered human retinal pigment epithelial cells (ARPE-19) to synthesize human cell-derived BMP-2 (hBMP-2) within a collagen-based bone graft matrix. ARPE-19 cells were transduced using a lentiviral vector encoding BMP-2 and subsequently cultured in a porcine-collagen mixed graft material. The efficacy of this hBMP-2-enriched graft was evaluated in a rat calvaria defect model over 6 weeks, with comparisons made against empty defects and grafts lacking hBMP-2. Micro-computed tomography analysis revealed improved bone regeneration parameters in the hBMP-2 group compared with those in the graft-only group, although statistical significance was limited to the trabecular number, which approached borderline significance (p < 0.1). Histological analysis corroborated these findings, revealing significantly enhanced new bone formation but only a reducing tendency of residual graft material in the hBMP-2 group. This study presents a promising approach for bone regeneration by utilizing genetically engineered human cells to produce BMP-2 within clinically available bone graft materials, potentially mitigating the high-dose requirements and associated complications of conventional BMP-2 therapies.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"23 ","pages":"22808000251326799"},"PeriodicalIF":3.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144010463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating the effect of printing parameters on the performance of resin occlusal splints for a sustainable dentistry.","authors":"Ashish Kaushik, Ramesh Kumar Garg, Ravinder S Saini, Francesco Bennardo, Artak Heboyan","doi":"10.1177/22808000251333700","DOIUrl":"https://doi.org/10.1177/22808000251333700","url":null,"abstract":"<p><p>Bruxism affects millions worldwide, leading to dental damage like worn teeth and tooth loss. Resin 3D printing presents a promising method for creating intricate, comfortable, and durable occlusal splints. This study examines how printing parameters-layer thickness, orientation angle, and curing time-affect the mechanical (compressive strength, wear rate, impact strength) and physical (water sorption, surface roughness, dimensional accuracy) properties of occlusal splints made from a methacrylate-based resin. A total of 120 specimens were produced according to American Society for Testing and Materials (ASTM) standards using different parametric combinations. The response surface methodology (RSM) was applied to optimize key parameters. The optimum printing parameters for compressive strength include a layer height of 16.5 mm, curing time of 93.6 min, an orientation angle of 12.8º, yielding a compressive strength of 9.05 MPa, wear rate of 159 mm³/min, and impact strength of 71.58 J/m. Similarly, the optimum results for minimum surface roughness (8.013 microns), maximum dimensional accuracy (97.67 and minimum water sorption (0.386%) are achieved at a layer thickness of 16 mm, curing time of 93 min, and orientation angle of 12º. Results show that optimizing resin 3D printing parameters for occlusal splints significantly reduces production costs, particularly in regions with limited access to dental care, while promoting sustainable dental solutions by minimizing the environmental impact of traditional manufacturing methods and enhancing the efficiency of splint production.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"23 ","pages":"22808000251333700"},"PeriodicalIF":3.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144006739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced bone regeneration with cerium-doped bioactive glasses: In vitro and in vivo study.","authors":"Roberta Salvatori, Alexandre Anesi, Luigi Chiarini, Mattia Di Bartolomeo, Arrigo Pellacani, Chiara Cavazzoli, Alfonso Zambon, Gigliola Lusvardi","doi":"10.1177/22808000251326794","DOIUrl":"https://doi.org/10.1177/22808000251326794","url":null,"abstract":"<p><strong>Background: </strong>Bioactive cerium-doped (Ce-BGs) glasses with proven antioxidant properties, which may reduce post-implant oxidative stress, were studied in vitro and in vivo to evaluate their application in bone regeneration. Based on the Kokubo (K) composition, they contain 3.6 and 5.3 mol% cerium (referred to as K3.6 and K5.3, respectively).</p><p><strong>Methods: </strong>Ce-BGs were synthesized by melting and sieved to produce granules (size range = 200-500 µm). In vitro studies were conducted against MLO-Y4 cells using direct Neutral Red (NR) and indirect Bromo-2-deoxyUridine (BrdU) assays to assess cell viability and proliferation respectively. In vivo studies were carried out using a New Zealand white rabbit model to evaluate bone healing potential.</p><p><strong>Results and discussion: </strong>NR results showed a significant increase in cell viability for Ce-BGs: 77% for K and 79 and 85% for K3.6 and K5.3, after 24 h. After 72 h, cell viability decreased for K to 58% and increased for K3.6 and K5.3 (76% and 116% respectively). Cerium inhibits cell proliferation in BrdU assay as explainable by the increased durability of Ce-BGs. In vivo studies, after 30 and 60 days, revealed a delayed degradation for Ce-BGs that can stimulate the osteo-regeneration without inflammatory or degenerative effects. Moreover, the new bone area (NBA) was higher for Ce-BGs compared to control; after 60 days 32% for K5.3 versus 21% for K.</p><p><strong>Conclusions: </strong>Ce-BGs granules show improved direct cytocompatibility in vitro and enhance the long-term bone remodeling process in vivo, contributing to a more controlled and effective bone healing compared to the K granules. This improved behavior can be linked to the antioxidant and anti-inflammatory properties of cerium, that can assist bone regeneration and reduce implant-associated inflammation, and to their slower dissolution rate that supports the controlled release of ions. These results suggest Ce-BGs as a promising device for therapeutic applications on <i>hard</i> tissues.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"23 ","pages":"22808000251326794"},"PeriodicalIF":3.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144025965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating the early osteogenic potential of strontium doped titanium alloy surface.","authors":"Ali Alenezi, Mohamed Ahmed Alkhodary","doi":"10.1177/22808000251328992","DOIUrl":"https://doi.org/10.1177/22808000251328992","url":null,"abstract":"<p><p>The current study was conducted to test the early osteogenic potential of strontium (Sr) doped titanium-aluminum-vanadium (Ti-6Al-4V) alloy using an in vitro cell culture experiment. Thirty Ti-6Al-4V alloy sheets were sandblasted and etched with large grit acid. Of these, 10 alloy sheets represented group I, 10 sheets doped with Sr using a hydrothermal process represented group II, and 10 sheets also coated with Sr-supplemented hydroxyapatite using a hydrothermal process represented group III. The surfaces of the three groups were characterized by scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), and X-ray diffraction (XRD). The three groups were also compared in terms of their water contact angle, protein adsorption, and Sr ion release profile. Further, bone marrow mesenchymal cells (BMSCs) obtained from the femora of 10 Sprague Dawley rats were used for the in vitro cell culture experiment, and the viability of the cultured cells was evaluated using the MTT assay and confocal microscopy; in addition, their osteogenic potential was assessed using alkaline phosphatase ALP activity. For statistical analysis, data were analyzed using one-way analysis of variance (ANOVA; IBM SPSS statistics 23), and <i>p</i> < 0.05 was considered statistically significant. SEM images revealed that the three groups had surface roughness, and EDS and XRD revealed the success of incorporating Sr to their surfaces. Group III had the best contact angle, protein adsorption, and Sr ion release rate. The cell culture also revealed that the surfaces of the titanium alloy sheets in group III were the most viable and had the best osteogenic potential; however, there was no statistically significant difference between groups I and II. In Conclusion, Sr alone was not able to improve the osteogenic potential of titanium alloy surfaces.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"23 ","pages":"22808000251328992"},"PeriodicalIF":3.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144021745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Resveratrol-loaded PCL-PEG/GO/HAP biocomposite bone membranes: Evaluation of mechanical properties, release kinetics, and cellular response.","authors":"Betül Meryem Arpacay, Fatih Ciftci, Ali Can Özarslan, Mustafa Unal, Mine Kucak, Aslihan Yelkenci","doi":"10.1177/22808000251314087","DOIUrl":"10.1177/22808000251314087","url":null,"abstract":"<p><p>In this study, biocomposite membranes were developed by incorporating resveratrol (RSV)-loaded PCL-PEG composites, modified with graphene oxide (GO) and hydroxyapatite (HAP). The aim was to enhance hydrophilicity with GO and improve bioactivity with HAP. The release kinetics of RSV was evaluated by using Franz diffusion cells and compared with various kinetic models, including Korsmeyer-Peppas, Higuchi, and Baker, all of which showed high correlation coefficients (<i>R</i>²) close to 0.99. Mechanical tests was performed to determine the suitability of these membranes for tissue engineering applications. The composite membrane modified with GO and HAP exhibited tensile strength of 105.2 ± 5.8 MPa, tensile modulus of 3895 ± 159 MPa, elongation at break of 8.4 ± 0.9%, and toughness of 5.88 ± 0.46 MJ/m³. In vitro cell adhesion studies, visualized using DAPI fluorescence staining, demonstrated increased cell adhesion to the composite membranes over periods of 1, 3, 5, 7, and 14 days. These findings highlight the potential of the RSV-loaded PCL-PEG membranes, enhanced with GO and HAP, for applications in bone tissue engineering.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"23 ","pages":"22808000251314087"},"PeriodicalIF":3.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143080105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}