Journal of Applied Biomaterials & Functional Materials最新文献

{"title":"Human mesenchymal stem cell expansion on laminin-521 in serum-free and xeno-free culture conditions.","authors":"Halima Albalushi, Mohadese Boorojerdi, Elias Said, Halima Al Shehhi, Nihal Al Riyami, Mohammed Al Rawahi, Murtadha Al Khabori","doi":"10.1177/22808000251332110","DOIUrl":"https://doi.org/10.1177/22808000251332110","url":null,"abstract":"<p><p>Laminin-521 (LN521) is a crucial adhesion protein found in natural stem cell niches and plays an important role in maintaining human pluripotent stem cell (PSC) properties. This study aimed to investigate the effects of LN521 on human umbilical cord-derived mesenchymal stem cell (UC-MSC) characteristics in Serum-free and Xeno-free culture conditions as a step toward clinical application. In our experiment isolated UC-MSC via explant method were expanded as a homogeneous monolayer and morphologically, presented typical MSC-like morphology (spindle-shaped) from passage three to six when cultured on either LN521 or CELLstart™. Almost, 90% confluency was reached after 4 days of culture with an EI of approximately 11.2 with no statistically significant differences on LN521 and CELLstart™ in all six passages. Phenotypic characterization of UC-MSC cultured on LN521 or CELLstart™ using flow cytometry, along with the expression of the same biomarkers in gene level analyzed by quantitative reversed transcription revealed identical CD73, CD90, CD105, CD34, CD45, CD19, CD14, and HLA-DR expression pattern at passages three and six in both LN521 and CELLstart™. Moreover, UC-MSC cultured in the presence of LN521 and CELLstart™ showed the same adipogenesis, chondrogenesis and osteogenesis differentiation potential, and normal chromosome structure highlighting genetic stability. Ultimately, LN521 is comparable to CELLstart™ in supporting UC-MSC expansion and maintaining their characteristics in serum-free and xeno-free culture conditions.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"23 ","pages":"22808000251332110"},"PeriodicalIF":3.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144002088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioactive oligopeptides and the application in skin regeneration and rejuvenation.","authors":"Qiulin He, Youguo Liao, Yaru Wu, Huahui Zhang, Xiaohui Long, Yuxiang Zhang","doi":"10.1177/22808000251330974","DOIUrl":"https://doi.org/10.1177/22808000251330974","url":null,"abstract":"<p><p>Oligopeptides, composed of 2-10 amino acid residues, are protein fragments with unique structural characteristics, including small molecular size, high biocompatibility, and modifiable functional groups. These features endow oligopeptides with excellent permeability, safety, and versatile biological activities, making them widely applicable in disease treatment, drug delivery, and skincare. In particular, oligopeptides have emerged as advanced ingredients in skincare, offering anti-aging, anti-wrinkle, and whitening effects by regulating key biological processes such as collagen synthesis, antioxidant defense, and melanin production. This review comprehensively discusses the structural properties, functional mechanisms, and diverse applications of oligopeptides and their derivatives, highlighting their potential in skin regeneration, rejuvenation, and anti-aging medicine. By providing insights into the latest advancements, this review aims to serve as a valuable reference for future research and development in oligopeptide-based therapeutics and skincare innovations.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"23 ","pages":"22808000251330974"},"PeriodicalIF":3.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144000586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of Ion Leachable Resin Composites: Time-Dependent Water Sorption, Solubility and Hygroscopic Expansion.","authors":"Abdullah Alshehri, Ahmed A Almokhatieb, Mohammed Mustafa, Khaled Abid Althaqafi, Waseem Waleed Radwan, Mohammad Khursheed Alam","doi":"10.1177/22808000251348969","DOIUrl":"https://doi.org/10.1177/22808000251348969","url":null,"abstract":"<p><strong>Aim: </strong>This study aimed to assess the time-dependent water sorption, solubility and hygroscopic expansion of experimental resin composites modified with three different types of ion-leachable glasses (ILGs): 45S5 Bioglass (BG), Fluoride-containing glass (F9) and Experimental fluoride-phosphate glass (F9X), incorporated in varying weight percentages (5%, 10% and 15%).</p><p><strong>Materials and methods: </strong>A 50:50 Bis-GMA/TEGDMA-based resin matrix was loaded with each ILG type in 5, 10 and 15 wt% and compared against a control without filler. Disc-shaped specimens (<i>n</i> = 3 per group; total = 39) were fabricated using a stainless-steel mould and cured using an LED light-curing unit (1200 mW/cm², 20 s per side). The water sorption and solubility were evaluated using a modified ISO 4049 protocol over a 12-week immersion period in distilled water at 37°C, followed by an 8-week desorption phase. Hygroscopic expansion was evaluated through volume change using a digital micrometre. Data were statistically analysed using one-way ANOVA and Tukey's post-hoc test.</p><p><strong>Results: </strong>All ILG-containing composites showed significantly increased water sorption compared to the control, with the BG-15 group demonstrating the highest sorption (3.37% ± 0.09) and expansion. Solubility increased with ILG concentration, especially in the BG and F9X groups. Hygroscopic expansion correlated positively with water uptake. No significant changes were observed in specimen mass after desorption in low filler groups.</p><p><strong>Conclusion: </strong>The incorporation of ILGs into resin composites significantly altered their water uptake and dimensional stability. While these effects could compromise long-term mechanical properties, the resulting hygroscopic expansion may aid in reducing microgaps and secondary caries at restoration margins. Veneering ILG-containing composites with conventional materials is recommended to limit degradation. These findings contribute novel insights into time-dependent dimensional behaviour of bioactive composites.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"23 ","pages":"22808000251348969"},"PeriodicalIF":3.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144567464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An assessment of synthesis technique for porous nano and micro fibrous wound dressings with natural oil supplements.","authors":"Meltem Kum, Levent Sendogdular, Selda Topcu Sendogdular","doi":"10.1177/22808000251314106","DOIUrl":"10.1177/22808000251314106","url":null,"abstract":"<p><p>For millennia, aloe vera (AV) and eucalyptus oil (EO) have been recognized as natural sources of healing and have been utilized for medicinal purposes in the realm of health. As an attempt to treat pressure sores, AV and eucalyptus oil were added as supplements to biocompatible and biodegradable poly (ethylene oxide) (PEO) polymer to synthesize nano and micro fibrous wound dressings by the electrospinning process. Additive solubility in polymeric matrix is the key parameter to achieve the synthesis of homogeneous fibers with controlled release of therapeutic oils, cure and humidity; therefore, lecithin as herbal (soybean) based emulsifier was used to control additive/polymer solubility. In this study, fibrous dressing in mat form with antioxidant activity was successfully obtained with the addition of natural AV and EO in PEO polymer solutions through electrospinning technique. Subsequently, the synthesized fibers were examined via scanning electron microscopy (SEM), thermogravimetric analysis (TGA), moisture absorption and UV-Vis spectroscopy. SEM imaging demonstrated the formation of randomly-oriented and beadless fibers with size of 0.48 ± 0.23 µm out of PEO/AV/EO/Lecithin blend and also with the addition of lecithin, fiber thicknesses were observed to be increasing. Moisture absorption analysis revealed that the weight of fibrous mat was affected by the humidity of the ambient environment. Relative humidity for 7 days ranged between 32% and 37% and it was observed that lecithin content increased the moisture retention rate by 50%. Uv-Vis results suggested that a more regular performance has been achieved with lecithin being involved in terms of timely manner changes; therefore, the contrast of samples between hours and days became more distinctive. PEO/AV/EO/Lecithin nanofiber also indicated antibacterial ability against <i>Escherichia coli</i> with approximately 18.5 mm diameter of inhibition. This research proves that the potential for developing biocompatible wound dressings with long-lasting moisture to the wound is possible through the use of these natural healing agents made homogeneously distributed through structure by the use of emulsifier.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"23 ","pages":"22808000251314106"},"PeriodicalIF":3.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143604802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Non-peroxide eggshell-based experimental material: Effects on the color and composition of human dental enamel.","authors":"Carolina Torres-Rodríguez, Jennifer González García, Pedro Álvarez-Lloret, Edgar Delgado-Mejía","doi":"10.1177/22808000251356939","DOIUrl":"https://doi.org/10.1177/22808000251356939","url":null,"abstract":"<p><p>Alternative calcium phosphates are strong candidates as tooth-whitening to mitigate the adverse effects of hydrogen peroxide (HP). This study examines changes in the color and chemical structure of human enamel induced by a biomimetic calcium phosphate-based bleaching ceramic derived from chicken eggshell, known as the experimental remineralizing Substance (ERS). Forty human molars were assigned to four groups: G1: storage group; G2: treated with 35% HP; G3: treated with ERSs; and G4: treated with HP followed by ers. CIE color coordinates (<i>L</i>*, <i>a</i>*, <i>b</i>*) were recorded before and after treatment using a dental spectrophotometer to calculate color differences (Δ<i>E*_ab</i>, Δ<i>E₀₀</i>) and the whiteness index<math><mrow><mo>(</mo><mi>W</mi><msub><mi>I</mi><mi>D</mi></msub><mo>)</mo></mrow></math> which were later compared with previously established perceptibility (PT) and acceptability (AT) thresholds. After each treatment, enamel surface powders from each specimen were analyzed using attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR) and X-ray diffraction (XRD). Statistical analyses included one-way ANOVA and simple linear regression for color measurements, as well as one-way ANOVA, Shapiro-Wilk, Kruskal-Wallis, and Holm-Bonferroni tests for chemical composition. The results indicated a significant decrease in <i>L</i>* (<i>p</i> = 0.01) and <i>b</i>* (<i>p</i> = 0.03) values in G3. The highest mean values of <i>∆E*_ab</i> and Δ<i>E₀₀</i> (<i>p</i> ⩽ 0.05) exceeded PT and AT indicating good effectiveness. ATR-FTIR analysis revealed that certain phosphate bands in G3 remained unchanged compared to G1 and G2. Additionally, carbonate substitution at the A and B-positions was reduced, and the degree of mineralization increased compared to G2. XRD patterns showed diffraction peaks linked to hydroxyapatite crystals in all experimental groups. All treatments reduced crystallite size, and this effect was not reversed in G3. Thus, this study demonstrates that ERS achieved a superior whitening effect compared to HP, without altering the chemical composition or crystalline structure of human enamel. These findings suggest the potential of using ERS as a safer alternative to conventional peroxide-based whitening agents.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"23 ","pages":"22808000251356939"},"PeriodicalIF":3.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144789239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chitosan-coated titanium screws: Enhancing biofilm resistance, mechanical stability, and osseointegration in orthopedic implants.","authors":"Niranjan Ghimire, Manish Rayamajhi, Yuyu Sun, Ying Deng","doi":"10.1177/22808000251358057","DOIUrl":"https://doi.org/10.1177/22808000251358057","url":null,"abstract":"<p><p>Orthopedic implant-associated infections, primarily caused by biofilm-forming <i>Staphylococcus aureus</i>, pose significant clinical challenges. These infections often lead to implant failure, prolonged antibiotic treatments, and an increased risk of revision surgeries, emphasizing the need for effective biofilm-resistant implant materials. In this study, we present a dual-functional titanium screw (Ti-S) grafted with chitosan (Cs), a biocompatible polymer known for its osteogenic and antimicrobial properties while maintaining mechanical integrity. The chitosan-modified titanium screw (Cs-Ti-S) was prepared via chemical immobilization to enhance resistance to biofilm formation while promoting osseointegration and preserving biomechanical integrity. Biomechanical testing confirmed that chitosan modification did not compromise mechanical performance, as Cs-Ti-S exhibited a torsional yield strength of 1.70 ± 0.00 Nm compared to 1.76 ± 0.05 Nm for unmodified titanium screws (Un-Ti-S), and an axial pullout force of 68.66 ± 14.36 N for Cs-Ti-S versus 70.33 ± 9.71 N for Un-Ti-S. Micro-scratch tests revealed similar hardness values (1.26 ± 0.03 GPa for Cs-Ti-S vs. 1.40 ± 0.07 GPa for Un-Ti-S) and scratch resistance, ensuring surface durability. Gene expression analysis showed upregulated β1-integrin on Cs-Ti-S at 24 h post-infection, indicating improved osteoblast adhesion. Scanning electron microscopy (SEM) analysis confirmed significantly reduced bacterial biofilm formation on Cs-Ti-S. Moreover, the combination of povidone-iodide (PI) treatment on Cs-Ti-S surfaces significantly inhibited biofilm formation over 7 days, unlike Un-Ti-S, which retained significant adhesion. These results suggest chitosan grafting as a scalable, non-antibiotic strategy to enhance antimicrobial resistance and osseointegration.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"23 ","pages":"22808000251358057"},"PeriodicalIF":3.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144955245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel 2D MXenes biocomposite for the removal of emerging organic contaminants from wastewater.","authors":"Sweta Raj, Divya Bajpai Tripathy, Lalit Prasad, Anil Kumar","doi":"10.1177/22808000251360553","DOIUrl":"https://doi.org/10.1177/22808000251360553","url":null,"abstract":"<p><p>Growing ecological and public health issues brought on by the increasing presence of novel organic contaminants in wastewater need the development of innovative remediation solutions. It's usually challenging for conventional treatment methods to effectively collect these contaminants, which include pharmaceuticals, personal care products, and industrial chemicals. Scientists are, therefore, concentrating on innovative material to increase the efficiency of adsorption and removal. Because they facilitate interaction with a range of organic pollutants, 2D MXenes' unique structural and chemical properties have drawn interest from these materials. MXenes are very excellent adsorbents for a variety of contaminants because of their large surface area, many terminal groups, and distinctive 2D layer architectures. Polyfluoroalkyl substances (PFAS), dyes, antibiotics (tetracycline, sulfonamides, and ciprofloxacin), amitriptyline, verapamil, carbamazepine, 17α-ethinyl estradiol, antibiotic resistance genes (ARGs), diclofenac, ibuprofen heavy metals, and other contaminants have all been claimed to be eliminated by MXenes. Recent studies propose the formulation of MXene-based biocomposites, which not only harness the high surface area and electrical conductivity of MXenes but also integrate biodegradable components to promote eco-friendliness. This work explores the potential of novel 2D MXenes biocomposites in addressing the critical challenge of wastewater treatment, focussing on their efficiency, and sustainability in removing emerging contaminants.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"23 ","pages":"22808000251360553"},"PeriodicalIF":3.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144955273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanomaterials in abdominal aortic aneurysm: From targeted therapy to smart vascular repair.","authors":"Xiya Yan, Zhihan You, Weimin Zhou, Wenpeng Zhao, Jiacong Qiu","doi":"10.1177/22808000251379140","DOIUrl":"https://doi.org/10.1177/22808000251379140","url":null,"abstract":"<p><p>Abdominal aortic aneurysm (AAA), defined as a permanent and often asymptomatic dilatation of the abdominal aorta, poses a significant threat of rupture with high mortality, yet lacks effective pharmacological interventions for stabilisation or regression. Current surgical options are invasive or require strict anatomical suitability, leaving patients with small aneurysms under passive surveillance. This critical gap underscores the urgent need for novel therapeutic strategies. Nanomedicine has emerged as a promising approach, offering solutions for targeted drug delivery, precise imaging, and dynamic monitoring of AAA progression. This review comprehensively analyses recent advances in nanomaterial-based systems for AAA management. We classify and discuss key nanocarriers-inorganic nanomaterials, organic nanomaterials, and hybrid nanomaterials-highlighting their unique design strategies, targeting mechanisms, and therapeutic functions. Specific applications include the targeted delivery of anti-inflammatory agents, modulation of matrix metalloproteinase activity via siRNA delivery, protection of vascular smooth muscle cells (VSMCs) from oxidative stress and apoptosis, and strategies for in situ vascular repair and regeneration. Furthermore, the role of nanomaterials in enhanced diagnostics and intelligent sensing for rupture risk prediction is explored. Despite encouraging preclinical results, challenges regarding long-term biosafety, translatability from rodent models to human pathophysiology, and optimisation of hemodynamic delivery remain significant hurdles. Future directions involve closed-loop theranostic systems, artificial intelligence integration, hemodynamic-optimised nanoparticle design, and exploring gene-editing nanocarriers. This review concludes that engineered nanomaterials hold substantial potential to transform AAA management from passive monitoring to active prevention and precision therapy, paving the way for future clinical translation.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"23 ","pages":"22808000251379140"},"PeriodicalIF":3.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145137579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The influence of stainless steel crowns placed on primary teeth on biochemical parameters in GCF: A systematic review.","authors":"Mario Alberto Alarcón-Sánchez, Julieta Sarai Becerra-Ruiz, Ruth Rodríguez-Montaño, Sarah Monserrat Lomelí-Martínez, Luca Fiorillo, Artak Heboyan","doi":"10.1177/22808000251335403","DOIUrl":"10.1177/22808000251335403","url":null,"abstract":"<p><strong>Objective: </strong>The aim of this systematic review was to investigate, identify, and summarize the existing literature on the levels of proinflammatory cytokines and chemokines present in gingival crevicular fluid (GCF) of primary teeth restored with stainless steel crowns (SSC) versus control teeth.</p><p><strong>Materials and methods: </strong>The systematic review was registered in the Open Science Framework (ID): 10.17605/OSF.IO/39U4D. In addition, it was prepared following the guidelines of the Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA). Five electronic databases were used to identify studies for this systematic review: PubMed, Scopus, ScienceDirect, Web of Science, and Google Scholar, from January 10, 1999, to September 15, 2024. The risk of bias in the included studies was assessed using Joanna Briggs Institute (cross-sectional studies) and the Cochrane Risk of Bias for Randomized Trials (RoB 2.0) in randomized clinical trials.</p><p><strong>Results: </strong>The review includes four studies (two cross-sectional and two randomized clinical trials). A total of 75 children aged 3 to 10 years were studied. GCF samples were taken from 98 upper and lower molars rehabilitated with SSC and control teeth (without SSC). ELISA analyzed all samples. This way, the levels of four proinflammatory cytokines and chemokines, IL-1β, IL-6, MIP-1α, and MIP-1β, were determined. The studies reported significant differences between both study groups: IL-1β: 27.30 versus 23.56 <i>p</i> < 0.05; MIP-1α: 682.55 versus 197.60 <i>p</i> < 0.05; and MIP-1β: 884.35 versus 287.85, <i>p</i> < 0.05.</p><p><strong>Conclusions: </strong>This systematic review provides a comprehensive and current overview of the concentrations of proinflammatory cytokines and chemokines present in GCF, providing new insights into the pathogenesis of gingival inflammation in children with SSC. IL-1β, MIP-1α, and MIP-1β levels increased in the GCF of upper and lower molars rehabilitated with stainless steel crowns compared to control primary teeth.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"23 ","pages":"22808000251335403"},"PeriodicalIF":3.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143966698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regenerative effect of human cell-derived BMP-2 cultured within a collagen bone graft in a rat calvaria defect model.","authors":"Keun-Suh Kim, Euna Pi, Sang-Jun Park, Seungyoon Lee, Inyoung Choi, Taeuk Kim, Hyo-Jung Lee, Yang-Jin Yi","doi":"10.1177/22808000251326799","DOIUrl":"https://doi.org/10.1177/22808000251326799","url":null,"abstract":"<p><p>Bone morphogenetic protein-2 (BMP-2) is a potent osteoinductive factor; however, current clinical applications using <i>Escherichia coli</i>-derived recombinant human BMP-2 are constrained by structural deficiencies and high-dose requirements, which increases the risk of adverse effects. In this study, we aimed to address these limitations by developing a novel approach leveraging genetically engineered human retinal pigment epithelial cells (ARPE-19) to synthesize human cell-derived BMP-2 (hBMP-2) within a collagen-based bone graft matrix. ARPE-19 cells were transduced using a lentiviral vector encoding BMP-2 and subsequently cultured in a porcine-collagen mixed graft material. The efficacy of this hBMP-2-enriched graft was evaluated in a rat calvaria defect model over 6 weeks, with comparisons made against empty defects and grafts lacking hBMP-2. Micro-computed tomography analysis revealed improved bone regeneration parameters in the hBMP-2 group compared with those in the graft-only group, although statistical significance was limited to the trabecular number, which approached borderline significance (p < 0.1). Histological analysis corroborated these findings, revealing significantly enhanced new bone formation but only a reducing tendency of residual graft material in the hBMP-2 group. This study presents a promising approach for bone regeneration by utilizing genetically engineered human cells to produce BMP-2 within clinically available bone graft materials, potentially mitigating the high-dose requirements and associated complications of conventional BMP-2 therapies.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"23 ","pages":"22808000251326799"},"PeriodicalIF":3.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144010463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}