Journal of Applied Biomaterials & Functional Materials最新文献

{"title":"Tribocorrosion response of Fe-modified titanium alloys in simulated body fluids with varying glucose concentrations.","authors":"Nicola Rundora, Desmond Klenam, Oluwasegun Falodun, Maria Mathabathe, Vusumuzi Sibanda, Michael Bodunrin","doi":"10.1177/22808000261431504","DOIUrl":"10.1177/22808000261431504","url":null,"abstract":"<p><p>This study evaluates the tribocorrosion behavior of Fe-modified titanium alloys (Ti-3Fe, Ti-4.5Al-1V-3Fe, Ti-6Al-1V-3Fe) in comparison with Ti-6Al-4V in Hank's balanced salt solution (HBSS) simulating normal (0 mg/dL), pre-diabetic (100 mg/dL) and diabetic (200 mg/dL) physiological glucose conditions. Tribocorrosion tests were conducted using a reciprocating sliding configuration under open-circuit potential (OCP) and potentiostatic control (+0.5 V vs Ag/AgCl) to assess passive film stability, depassivation-repassivation kinetics and wear-corrosion synergy. Mechanical sliding induced cathodic OCP transients due to passive film rupture, with Ti-3Fe showing the largest potential drop (0.61 V, from -0.42 to -1.03 V) and negligible recovery (<0.01 V), indicating poor repassivation. In contrast, Ti-6Al-1V-3Fe and Ti-6Al-4V exhibited anodic OCP recovery of +0.059 and +0.095 V, reflecting enhanced passive film reformation. Under potentiostatic conditions, Ti-3Fe recorded peak sliding current densities of 6.4 × 10⁻⁴ A, while Ti-6Al-1V-3Fe and Ti-6Al-4V remained below 4.3 × 10⁻⁴ A, indicating higher resistance to depassivation. Increasing glucose concentration progressively destabilized the electrochemical response, with OCP fluctuation ranges increasing from 0.05 V in normal HBSS to 0.09 V in diabetic HBSS, accompanied by elevated sliding current densities and reduced post-sliding recovery. Wear analysis showed the lowest specific wear rate for Ti-6Al-4V (1.68 × 10⁻⁶ mm³/N·m) in normal HBSS, while Ti-3Fe consistently exhibited the highest material loss across all environments. The results show that alloy composition and glucose concentration in physiological media significantly influence tribocorrosion behavior, with Al-V-containing alloys demonstrating superior electrochemical stability and wear resistance, while Fe-rich titanium alloys exhibit greater susceptibility, particularly under diabetic conditions.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"24 ","pages":"22808000261431504"},"PeriodicalIF":2.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147838230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydrogels for 3D-printed breast scaffolds on esthetic, functional, and oncological safety: A review.","authors":"Zhuoyue Li, Shuqin Wang, Qian Tan","doi":"10.1177/22808000261431929","DOIUrl":"10.1177/22808000261431929","url":null,"abstract":"<p><p>Breast volume loss after cancer surgery severely affects patients' physical and psychological well-being, while traditional reconstruction methods such as prosthetic implantation and autologous tissue transfer are limited by complications, donor-site morbidity, and poor long-term stability. To address these issues, this review summarizes recent advances in hydrogel-based 3D-printed scaffolds for breast reconstruction. The review focuses on the use of different 3D bioprinting techniques in scaffold manufacture and compares natural and synthetic hydrogels in terms of biocompatibility, mechanical properties, and biodegradability. It also involves core design principles for scaffolds to meet the unique anatomical and functional requirements of the breast, including tunable softness, promotion of vascularization, and tissue integration. Furthermore, recent applications of hydrogel-based 3D printing in adipose tissue regeneration and nipple-areola complex reconstruction are discussed in detail. The findings indicate that natural hydrogels provide advantages in cell viability and differentiation due to their biocompatibility, while synthetic hydrogels offer tunability and structural stability for long-term support. Combining the two materials can enhance reconstruction outcomes. It further addresses challenges and prospective directions in 3D printing hydrogels in breast reconstruction, paving the way for innovative research to material selection and printing strategies, enhancing patient care, and improving quality of life.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"24 ","pages":"22808000261431929"},"PeriodicalIF":2.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147468141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultra-fine micronized human dermal matrix reprograms the chronic pressure-ulcer microenvironment to accelerate healing.","authors":"Hao Jin, Binna Shin, Daseul Kim, Yeji Seo, Yongjin Lee, Sang-Myoeng Lee","doi":"10.1177/22808000261460977","DOIUrl":"https://doi.org/10.1177/22808000261460977","url":null,"abstract":"<p><p>Chronic pressure ulcers persist within a self-sustaining microenvironment marked by unresolved inflammation, inadequate neovascularization, and excessive extracellular-matrix (ECM) degradation, highlighting the need for injectable bioactive scaffolds that can be delivered precisely to reprogram compromised tissue. The ultra-fine micronized human acellular dermal matrix (UFM-hADM; ∼70 μm) is designed to remain suspendable and pass through narrow-gauge injection, enabling uniform distribution along irregular wound margins and undermined tissue planes where sheet-type matrices may have limited contact and conformability. Here, we evaluated perilesional injection of an UFM-hADM (∼70 μm) as an injectable ECM scaffold in a murine cyclic ischemia-reperfusion pressure-ulcer model. Compared to saline, UFM-hADM accelerated early wound closure and improved quality of healing at Day 7, with enhanced re-epithelialization and dermal regeneration accompanied by increased collagen deposition. Mechanistically, UFM-hADM shifted the wound milieu toward a pro-healing program by attenuating pro-inflammatory cytokines (TNF-α and IL-6) while increasing IL-10, promoting neovascularization and vascular maturation (upregulated VEGF, increased CD31 and α-SMA), and restoring balanced ECM remodeling (elevated COL1A1 and COL3A1 with suppression of MMP1 and MMP3). These coordinated molecular changes opposed inflammatory, protease-rich transcriptional signatures identified in human pressure ulcers through public transcriptomic reanalysis. In addition, exploratory observational use of Ministry of Food and Drug Safety (MFDS)-approved UFM-hADM for pressure ulcers, undertaken with patient consent as part of routine clinician-directed care, suggested acceptable tolerability and a favorable healing course during follow-up. Collectively, these findings provide mechanistic support for the adjunctive clinical use of UFM-hADM as a minimally invasive, human-derived injectable ECM adjunct that may facilitate pressure-ulcer healing through coordinated modulation of immune, vascular, and matrix responses.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"24 ","pages":"22808000261460977"},"PeriodicalIF":2.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148271556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D-printed ceramic scaffolds with colloidal silver and <i>Moringa oleifera</i> extracts for potential antimicrobial water treatment.","authors":"Ana Isabel Moreno Florez, Carolina Duque Uribe, Melissa Ocampo Avendaño, Maria Acosta, Omar Rodriguez Toloza, Laura Álvarez Gil, Alex Lopera, Alex Ossa, Alejandro Peláez-Vargas, Claudia García","doi":"10.1177/22808000261454655","DOIUrl":"https://doi.org/10.1177/22808000261454655","url":null,"abstract":"<p><p>Ceramic filters are an effective, low-cost solution for water potabilization, particularly in rural communities. The integration of 3D printing has advanced this traditional approach by enabling precise control over design parameters that govern filtration efficiency. Gyroid-type ceramic scaffolds for water treatment were fabricated by clay-based extrusion 3D printing. Two ceramic pastes (ECP 1 and ECP 2) containing kaolin, attapulgite, alumina, and feldspar were formulated; ECP 2 incorporated 10 wt% activated carbon as a sacrificial porogenic phase. Rheological characterization using rotational rheometry and Bingham model fitting identified 40 wt% water as optimal for extrusion. Sintering at 1100°C produced interconnected open-pore networks in ECP 2 (apparent porosity: 23.6%; water absorption: 11.8%). Scaffolds were functionalized with silver nanoparticles (AgNPs) and ethanolic extracts of <i>Moringa oleifera</i>. AgNPs exhibited surface plasmon resonance at 422 nm; silver leaching was 0.00282 ppm (WHO limit: 0.1 ppm). Ethanolic extract E2 (80% v/v) showed significantly lower MIC (0.175 g/mL) than E1 (0.35 g/mL; <i>p</i> < 0.05) and bactericidal activity (MBC/MIC ⩽ 4.0). Functionalized scaffolds (EG) reduced biofilm formation by 80%-95% versus controls at 24 h (MTT assay) and maintained cellular compatibility above the 80% ISO 10993-5 threshold at 7 days. This combination of properties positions them as a highly promising alternative for advanced ceramic water filters.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"24 ","pages":"22808000261454655"},"PeriodicalIF":2.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148277398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A biomimetic oxygen-generating nanoplatform for enhanced sonodynamic immune activation.","authors":"Haiqin Liao, Shiyu Ding, Ming Zhang, Quanliang Shang, Zhigang Wang","doi":"10.1177/22808000261464863","DOIUrl":"https://doi.org/10.1177/22808000261464863","url":null,"abstract":"<p><p>BackgroundSonodynamic therapy (SDT) has emerged as a promising strategy for cancer treatment; however, its therapeutic efficacy is significantly limited by the hypoxic tumor microenvironment, particularly in breast cancer. To address this limitation, we developed a biomimetic nanoplatform capable of generating oxygen within the tumor microenvironment to enhance SDT performance.MethodsPoly (lactic-co-glycolic acid) (PLGA) nanoparticles were co-loaded with catalase and the sonosensitizer IR780 and subsequently coated with 4T1 cancer cell membranes (CIP@4T1m NPs). A series of <i>in vitro</i> and <i>in vivo</i> experiments were conducted to evaluate tumor-targeting capability, hypoxia alleviation, singlet oxygen (¹O₂) generation, antitumor efficacy, induction of immunogenic cell death (ICD), and activation of antitumor immune responses.ResultsThe resulting CIP@4T1m NPs were successfully fabricated and exhibited preferential accumulation in 4T1 tumor cells and orthotopic 4T1 tumor-bearing mice. Both <i>in vitro</i> and <i>in vivo</i> studies demonstrated that the nanoplatform partially relieved tumor hypoxia and significantly enhanced SDT-mediated ¹O₂ production and antitumor effects. Moreover, CIP@4T1m NPs combined with ultrasound induced ICD-associated changes, promoted dendritic cell maturation, facilitated the polarization of tumor-associated macrophages from the M2 to M1 phenotype, reduced regulatory T cell populations, and increased intratumoral CD8⁺T-cell infiltration.ConclusionThis work describes a biomimetic nanoplatform that integrates homologous targeting with enzymatic oxygen generation to enhance SDT efficacy and promote antitumor immune responses in an orthotopic 4T1 breast cancer model. The proposed strategy offers a potential approach to mitigate hypoxia-associated limitations in cancer therapy.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"24 ","pages":"22808000261464863"},"PeriodicalIF":2.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148382145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Partial restoration of fracture resistance in mandibular molars: A comparative study of Bio MTA+ and calcium-enriched mixture for furcal perforation repair.","authors":"Birgul Ozasir, Tufan Ozasir, Deniz Imamoglu, Donya Rahimi, Kamran Gulsahi","doi":"10.1177/22808000261468420","DOIUrl":"https://doi.org/10.1177/22808000261468420","url":null,"abstract":"<p><p>ObjectiveFurcal perforations are iatrogenic complications which compromise the biological and mechanical integrity of endodontically treated teeth. Although calcium silicate-based materials are widely used for perforation repair, their ability to restore fracture resistance remains unclear. This study aimed to evaluate the fracture strength of mandibular molars with simulated furcal perforations repaired using Bio MTA+ or calcium-enriched mixture (CEM).MethodSeventy-six extracted human mandibular molars were randomly allocated into four groups (n = 19): negative control (no perforation), positive control (perforation without repair), Bio MTA+ (perforation repaired with Bio MTA+) and CEM (perforation repaired with CEM). Standardised root canal treatments were performed on all specimens, followed by perforation and repair procedures where applicable. Fracture strength was measured using a universal testing machine at a crosshead speed of 1 mm/min. The data were analysed using one-way ANOVA and Tukey's HSD test.ResultsA statistically significant difference was observed among the groups (F(3,72) = 52.362, p < 0.001). The negative control group exhibited the highest fracture strength values, whereas the positive control group presented the lowest. Both Bio MTA+ and CEM groups demonstrated significantly higher fracture strength than the positive control group (p < 0.05), but significantly lower values than the negative control group (p < 0.05). No statistically significant difference was found between Bio MTA+ and CEM (p = 0.773).ConclusionFurcal perforations significantly reduce the fracture resistance of endodontically treated teeth. Repair with calcium silicate-based materials improves mechanical strength but does not restore it to the level of endodontically treated teeth without perforation. Bio MTA+ and CEM demonstrated comparable performance and may be considered suitable options for furcal perforation repair.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"24 ","pages":"22808000261468420"},"PeriodicalIF":2.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148421215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The influence of radiopacifying agents on the physical and biological properties of tricalcium silicate-based cement.","authors":"Reda Banon, Sadir Al Ansari, Luc Martens, Peter De Coster, Chris Vercruysse, Jakob Van Acker, Elanagai Rathinam, Sivaprakash Rajasekharan","doi":"10.1177/22808000261460351","DOIUrl":"https://doi.org/10.1177/22808000261460351","url":null,"abstract":"<p><p>This study evaluated the influence of different radiopacifying agents on the physical and biological properties of tricalcium silicate-based cement (TCS). Eight radiopacifiers-bismuth oxide (Bi₂O₃), zirconium oxide (ZrO₂), calcium tungstate (CaWO₄), barium sulphate (BaSO₄), tantalum oxide (Ta₂O₅), strontium fluoride (SrF₂), strontium titanate (SrTiO₃), and barium titanate (BaTiO₃)-were incorporated into TCS, with pure TCS serving as the control. Setting time, compressive strength, radiopacity, discolouration, fluid uptake, porosity, solubility, bioactivity, calcium ion release, pH, and cytotoxicity were evaluated. Most radiopacifiers reduced the initial setting time and solubility without significantly affecting the final setting time, compressive strength at 7 and 28 days, bioactivity, or pH. All radiopacifiers increased radiopacity and calcium ion release, while also increasing fluid uptake and porosity. Certain radiopacifiers were associated with increased cytotoxicity. Among the materials tested, BaTiO₃ achieved the highest composite score. In contrast, CaWO₄, BaSO₄, ZrO₂, SrTiO₃, SrF₂, and Ta₂O₅ demonstrated progressively inferior performance compared with pure TCS, with Bi₂O₃ showing the least favourable outcome.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"24 ","pages":"22808000261460351"},"PeriodicalIF":2.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148673568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tissue response, antimicrobial activity, and mechanical properties of calcium silicate cement prototypes for vital pulp therapies.","authors":"Elizabeth Luna-Jaramillo, Rubén Abraham Domínguez-Pérez, Otoniel Corrales-Lozano, Manuel López-Rodríguez, Rosa Martha Pérez-Serrano, Marina Vega-González, León Francisco Espinosa-Cristóbal, Roberto Gustavo Sánchez-Lara Y Tajonar","doi":"10.1177/22808000251408320","DOIUrl":"https://doi.org/10.1177/22808000251408320","url":null,"abstract":"<p><p>The use of hydraulic calcium silicate cements (HCSCs) is essential for vital pulp therapies (VPT). However, their high cost restricts access in low- and middle-income countries, preventing the widespread benefits of VPT. This study aimed to evaluate four previously developed and characterized low-cost HCSC prototypes by examining their biological properties, specifically the rat connective tissue response after implantation and antimicrobial activity against five strains of interest. Additionally, their compressive strength, bond strength, and microhardness, which are critical mechanical properties of materials used in VPT, were assessed. All HCSCs caused an inflammatory reaction, which decreased over time in all cases, with most reactions categorized as mild. When comparing the number of inflammatory cells at each time point, no significant differences were observed between the HCSCs and those compared to MTA Angelus. Regarding the fibrous capsule, its thickness gradually decreased, and all capsules ultimately had a thickness with no significant difference compared to those formed in the empty control group. No significant differences in antimicrobial activity were seen among the four prototypes, as they exhibited similar performance against the five tested strains. However, some prototypes showed significantly better performance compared to MTA Angelus. Concerning mechanical properties, most prototypes exhibited substantially higher compressive strength than MTA Angelus, with a gradual increase over time-though this increase was not always significant. Additionally, no prototype demonstrated significant differences in bond strength compared to each other or MTA Angelus. Microhardness also increased over time, with significant differences observed when comparing prototypes to MTA Angelus at each time point. These findings, along with previously reported data on their microstructure, composition, and physical properties, support the potential clinical use of these prototypes. However, further research is needed to evaluate their effectiveness in clinical settings.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"24 ","pages":"22808000251408320"},"PeriodicalIF":3.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145889341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular modulation of cell migration via chitosan-based hydrogels: Toward smart biomaterial for wound regeneration.","authors":"Atefe Hosseinkhani, Reyhaneh Molaei, Ali Roosta, Nazanin Mansouri Ghader Abad, Mostafa Saberian","doi":"10.1177/22808000261431513","DOIUrl":"10.1177/22808000261431513","url":null,"abstract":"<p><p>Chitosan-derived hydrogels offer a highly tunable scaffold for orchestrating the multifaceted process of wound repair, yet their full potential hinges on an integrated understanding of biochemical and biophysical cues. Here, we provide a concise yet comprehensive analysis of design parameters that govern cell-matrix interactions, focusing on polymer backbone modification, crosslinking density, and incorporation of bioactive ligands. We demonstrate how precise control of viscoelastic properties viscoelastic properties, including stiffness and stress relaxation, achieved through reversible covalent bonds and supramolecular assemblies regulates YAP/TAZ nuclear localization and downstream PI3K/Akt and MAPK/ERK signaling to differentially promote coordinated keratinocyte migration, fibroblast proliferation, and endothelial angiogenesis. Functionalization with RGD peptides and heparin-binding motifs further amplifies receptor-mediated adhesion and growth factor sequestration, creating a pro-regenerative microenvironment. We critically appraise the kinetic profiles of growth factor release and immunomodulatory payloads, underscoring the necessity of spatiotemporal precision in smart hydrogel systems and summarize preclinical and clinical evidence demonstrating accelerated wound closure in diabetic and burn models. Finally, we identify pivotal challenges including the quantitative decoupling of mechanical versus molecular influences in vivo, long-term biocompatibility of modified chitosan derivatives, and translation of high-throughput screening data into clinical contexts and propose a roadmap leveraging single-cell transcriptomics and advanced imaging, and AI-driven modeling to overcome translational challenges. This synthesis delivers actionable guidelines for the development of next-generation, personalized wound therapies.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"24 ","pages":"22808000261431513"},"PeriodicalIF":2.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147609020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibition of indole production and attenuation of reactive oxygen species to reduce biofilm development.","authors":"Mohammad Amin Monsef, William Thomas Ingram, Justyn Jaworski","doi":"10.1177/22808000261472676","DOIUrl":"https://doi.org/10.1177/22808000261472676","url":null,"abstract":"<p><p>Bacterial biofilms provide survival mechanisms distinct from free-floating cells. An important mediator of biofilm formation in <i>Escherichia coli</i> (<i>E. coli</i>) is indole, which is formed by tryptophanase. In our study, <i>E. coli</i> biofilm formation was investigated in the context of removing indole production pathways, reducing indole production <i>via</i> a tryptophanase inhibitor (N-acetyl tryptophan), and by supplementing indole. We examined factors including indole production, the extent of biofilm generation, and reactive oxygen species (ROS) formation. Suppression of indole production by knockout of tryptophanase and by inhibitor-mediated reduction in tryptophanase activity both reduced biofilm formation in <i>E. coli</i>. In addition, there were indications that N-acetyl tryptophan could contribute to the anti-biofilm effect by affecting indole level and also the possibility of modulating ROS levels.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"24 ","pages":"22808000261472676"},"PeriodicalIF":2.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148578477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}