Journal of Applied Biomaterials & Functional Materials最新文献

{"title":"Effects of composition and pH on the degradation of hyaluronate and carboxymethyl cellulose gels and release of nanocrystalline silver.","authors":"Colleen Nancy Ward, Payton E LeBlanc, Robert Edward Burrell","doi":"10.1177/22808000241257124","DOIUrl":"https://doi.org/10.1177/22808000241257124","url":null,"abstract":"<p><p>Adhesions are fibrous tissue connections which are a common complication of surgical procedures and may be prevented by protecting tissue surfaces and reducing inflammation. The combination of biodegradable polymers and nanocrystalline silver can be used to create an anti-inflammatory gel to be applied during surgery. In this study, sodium hyaluronate and sodium carboxymethyl cellulose were added in concentrations from 0.25% to 1% w/v to aqueous nanocrystalline silver solutions to create viscous gels. Gels were loaded into dialysis cassettes and placed in PBS for 3 days. pH was adjusted using potassium phosphate monobasic and sodium hydroxide. Release of silver into the PBS was measured at several time points. Polymer degradation was compared by measuring the viscosity of the gels before and after the experiment. Gels lost up to 84% of initial viscosity over 3 days and released between 24% and 41% of the added silver. Gels with higher initial viscosity did not have a greater degree of degradation, as measured by percent viscosity reduction, but still resulted in a higher final viscosity. Silver release was not significantly impacted by pH or composition, but still varied between experimental groups.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"22 ","pages":"22808000241257124"},"PeriodicalIF":2.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141179543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Cnicus benedictus</i> extract-loaded electrospun gelatin wound dressing for treating diabetic wounds: An in vitro and in vivo study.","authors":"Jing Zhang, Shen Shen, Shijie Zhu, Fan Jia, Jin Li, Yu Sun","doi":"10.1177/22808000241245298","DOIUrl":"https://doi.org/10.1177/22808000241245298","url":null,"abstract":"<p><p>In the current study, <i>Cnicus benedictus extract</i> was loaded into electrospun gelatin scaffolds for diabetic wound healing applications. Scaffolds were characterized in vitro by mechanical testing, cell culture assays, electron microscopy, cell migration assay, and antibacterial assay. In vivo wound healing study was performed in a rat model of diabetic wound. In vitro studies revealed fibrous architecture of our developed dressings and their anti-inflammatory properties. In addition, <i>Cnicus benedictus extract-</i>loaded wound dressings prevented bacterial penetration. In vivo study showed that wound size reduction, collagen deposition, and epithelial thickness were significantly greater in <i>Cnicus benedictus</i> extract-loaded scaffolds than other groups. Gene expression studies showed that the produced wound dressings significantly upregulated VEGF and IGF genes expression in diabetic wounds.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"22 ","pages":"22808000241245298"},"PeriodicalIF":2.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140908703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Smooth muscle spheroids: Superior building blocks for bladder tissue engineering.","authors":"Dafni Planta, Tim Gerwinn, Valentin Baumgartner, Souzan Salemi, Daniel Eberli, Maya Horst","doi":"10.1177/22808000241305301","DOIUrl":"https://doi.org/10.1177/22808000241305301","url":null,"abstract":"<p><strong>Background: </strong>Congenital bladder disorders in children necessitate innovative approaches for bladder tissue regeneration, aiming to minimize complications associated with conventional therapies. This study focused on generating a cell-seeded scaffold using superior smooth muscle cells (SMCs) by exploring the potential of smooth muscle cell spheroids (3D SMCs) compared to conventionally cultured SMCs (2D SMCs) for bladder tissue engineering. Additionally, adipose-derived stem cells (ADSCs) were investigated for their impact on SMC proliferation and maturation, and pre-differentiated smooth muscle-like ADSCs (pADSCs) for their potential as alternative cell source.</p><p><strong>Methods: </strong>3D SMCs were seeded into a compressed collagen scaffold as monoculture and as co-culture with ADSCs or pADSCs and incubated for 2 weeks. Their contractile potential as well as proliferation and cell distribution within the scaffold were compared to conventionally cultured 2D SMCs by immunofluorescent staining and qRT-PCR.</p><p><strong>Results: </strong>3D SMCs in collagen scaffolds exhibited significantly superior cell distribution, proliferation, and contractile marker expression compared to 2D SMCs. While ADSCs showed limited impact, co-culture with pADSCs enhanced contractile marker expression, though not surpassing 3D SMC monoculture.</p><p><strong>Conclusion: </strong>For the first time, a collagen scaffold seeded with 3D SMCs was generated and evaluated. This study recommends 3D SMCs as optimal building blocks for bladder tissue engineering, highlighting the potential of pADSCs as an alternative cell source. These findings offer crucial insights for refining cell sources as well as culture techniques in pediatric bladder regeneration and provide a superior cell-seeded scaffold for further bladder tissue engineering experiments.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"22 ","pages":"22808000241305301"},"PeriodicalIF":3.1,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142800129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Platelet-rich fibrin in dentistry.","authors":"Ping Song, Dawei He, Song Ren, Lin Fan, Jiang Sun","doi":"10.1177/22808000241299588","DOIUrl":"https://doi.org/10.1177/22808000241299588","url":null,"abstract":"<p><strong>Objectives: </strong>To review the progress of Platelet Rich Fibrin (PRF) as a biomaterial in dentistry and to highlight its promising application as a safe and biocompatible autologous platelet concentrate. Publications were searched in GeenMedical, X-mol, GoogleScholar, and PubMed from October 2024 with no language restrictions. The literature was searched for relevant databases and journals on the use of PRFs in dentistry up to October 2024, and the inclusion criteria included randomized controlled trials, clinical trials, case series, and systematic reviews.</p><p><strong>Conclusion: </strong>PRF is a second-generation platelet concentrate that is sourced from oneself, has fewer adverse effects, and is simple and safe to prepare. These materials include growth factors and fibrin scaffolds, which are extensively utilized in regenerative medicine. By outlining PRF, we found that good results can be achieved when PRF is used to treat these conditions.</p><p><strong>Clinical significance: </strong>The application of PRF in dentistry is widespread, particularly in periodontal soft and hard tissue regeneration, oral lichen planus, and pulpal regeneration. This article reviews the background, classification, and preparation methods of PRFs, along with their dental applications. We anticipate further research on various PRF derivatives in the future, which will significantly improve the utilization of PRF in oral applications and offer fresh insights for diagnosing and treating oral diseases.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"22 ","pages":"22808000241299588"},"PeriodicalIF":3.1,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142715768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retraction Comparison of adhesive bond strength among fiber reinforced post and core with different cementation techniques: In vitro study.","authors":"","doi":"10.1177/22808000241273951","DOIUrl":"https://doi.org/10.1177/22808000241273951","url":null,"abstract":"","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"22 ","pages":"22808000241273951"},"PeriodicalIF":3.1,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141971212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial properties of copper tetraamine fluoride.","authors":"Veena Wenqing Xu, Iris Xiaoxue Yin, John Yun Niu, Ollie Yiru Yu, Mohammed Zahedul Islam Nizami, Chun-Hung Chu","doi":"10.1177/22808000241307803","DOIUrl":"https://doi.org/10.1177/22808000241307803","url":null,"abstract":"<p><strong>Background: </strong>A stable copper tetraamine fluoride (CTF) with low cytotoxicity has been developed for dental use.</p><p><strong>Objective: </strong>To investigate the antimicrobial effects of CTF against common microbes associated with dental caries and periodontal disease.</p><p><strong>Method: </strong>The minimum inhibitory concentrations (MIC) and minimum bactericidal/fungicidal concentrations (MBC/MFC) were used to evaluate the antimicrobial effects of CTF against eight common bacteria and one fungus associated with dental caries and periodontal disease. These nine microbes included cariogenic pathogens (<i>Streptococcus mutans</i>, <i>Streptococcus sobrinus</i>, <i>Lactobacillus acidophilus</i>, <i>Lacticaseibacillus casei</i>, <i>Actinomyces naeslundii</i> and <i>Candida albicans</i>), pulpitis-related bacteria (<i>Enterococcus faecalis</i>) and periodontal disease-related bacteria (<i>Porphyromonas gingivalis</i> and <i>Actinobacillus actinomycetemcomitans</i>). Transmission electron microscopy (TEM) was employed to examine the morphological changes of microbes with and without CTF treatment.</p><p><strong>Results: </strong>The MIC of CTF against nine microbes ranged from 80 ppm (<i>Lacticaseibacillus casei</i>) to 640 ppm (<i>Candida albicans</i> and <i>Enterococcus faecalis</i>). The MBC/MFC ranged from 320 ppm (<i>Lacticaseibacillus casei</i>) to 2560 ppm (<i>Candida albicans</i>). TEM revealed abnormal curvature of cell membranes, disrupted cell membranes, cytoplasmic clear zone, and cytoplasmic content leakage of the microbes treated with CTF.</p><p><strong>Conclusion: </strong>CTF has antimicrobial effects against common oral pathogens and presents a promising antimicrobial agent to aid management of dental caries and periodontal disease.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"22 ","pages":"22808000241307803"},"PeriodicalIF":3.1,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142846302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of Aleppo pine extract for skin burn treatment.","authors":"Najoua Salhi, Otman El Guourrami, Abdelaali Balahbib, Lamiae Rouas, Siham Moussaid, Amina Moutawalli, Fatima Zahra Benkhouili, Mouna Ameggouz, Riaz Ullah, Amal Alotaibi, Abdelhakim Bouyahya, Ching Siang Tan, Long Chiau Ming, My El Abbes Faouzi, Yahya Cherrah","doi":"10.1177/22808000241236020","DOIUrl":"10.1177/22808000241236020","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the <i>Pinus halepensis</i> extracts and determine its healing and antibacterial effects, and to evaluate the treatment of skin burns.</p><p><strong>Methods: </strong>Aqueous and ethanolic extracts and topical based on Aleppo pine plant extracts were prepared. Thirty male and female Wistar rats were used to study the cutaneous toxicity of extracts from the bark of <i>P. halepensis</i>. The extracts' healing potential for burn wounds were also assessed by evaluating the clinical and macroscopic aspects of the wounds. The antibacterial activity of crude extracts of <i>P. halepensis</i> as well as its wound healing abilities was verified in this investigation.</p><p><strong>Results: </strong>In animals with acute dermal toxicity, there were no signs of treatment-related toxicity or death. The extracts of these plants could be transformed into phytomedicines for the treatment of infected wounds. The results demonstrated that formulated ointments are successful in treating second-degree burns in rats and may be suitable for the short-term therapeutic treatment of second-degree burns.</p><p><strong>Conclusion: </strong>This study successfully answered our problem, regarding the efficacy of our extract for treating second-degree burns in rats. Further studies are needed to confirm these results by identifying the molecules responsible for these activities and examining their mechanism of action.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"22 ","pages":"22808000241236020"},"PeriodicalIF":2.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140094026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the purity of chitin from crustacean sources using deep eutectic solvents: A machine learning approach.","authors":"Sasireka Rajendran, Madheswaran Muthusamy","doi":"10.1177/22808000241248887","DOIUrl":"https://doi.org/10.1177/22808000241248887","url":null,"abstract":"<p><strong>Objective: </strong>Chitin a natural polymer is abundant in several sources such as shells of crustaceans, mollusks, insects, and fungi. Several possible attempts have been made to recover chitin because of its importance in biomedical applications in various forms such as hydrogel, nanoparticles, nanosheets, nanowires, etc. Among them, deep eutectic solvents have gained much consideration because of their eco-friendly and recyclable nature. However, several factors need to be addressed to obtain a pure form of chitin with a high yield. The development of an innovative system for the production of quality chitin is of prime importance and is still challenging.</p><p><strong>Methods: </strong>The present study intended to develop a novel and robust approach to investigate chitin purity from various crustacean shell wastes using deep eutectic solvents. This investigation will assist in envisaging the important influencing parameters to obtain a pure form of chitin via a machine learning approach. Different machine learning algorithms have been proposed to model chitin purity by considering the enormous experimental dataset retrieved from previously conducted experiments. Several input variables have been selected to assess chitin purity as the output variable.</p><p><strong>Results: </strong>The statistical criteria of the proposed model have been critically investigated and it was observed that the results indicate XGBoost has the maximum predictive accuracy of 0.95 compared with other selected models. The RMSE and MAE values were also minimal in the XGBoost model. In addition, it revealed better input variables to obtain pure chitin with minimal processing time.</p><p><strong>Conclusion: </strong>This study validates that machine learning paves the way for complex problems with substantial datasets and can be an inexpensive and time-saving model for analyzing chitin purity from crustacean shells.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"22 ","pages":"22808000241248887"},"PeriodicalIF":2.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140921974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineering VEGF-like peptide QKCMP promotes rapid endothelialization of blood vessels.","authors":"Haifeng Wang, Yi Huang, Chenhui Zhou, Fanyong Gong, Jiangyong Wang, Gao Chen","doi":"10.1177/22808000241301180","DOIUrl":"10.1177/22808000241301180","url":null,"abstract":"<p><p>Angiogenesis, which involves many essential processes, such as human reproduction, organ development, and wound healing, is regulated by multiple signaling pathways. QKCMP is a polypeptide with similar effects to vascular endothelial growth factor (VEGF), which promotes angiogenesis. In this study, zebrafish were treated with different concentrations of QKCMP, and it was found that QKCMP significantly promoted the growth of blood vessels. Human umbilical vein endothelial cells (HUVECs) was then treated with different concentrations of QKCMP, which proved that QKCMP could promote cell proliferation and inhibit cell apoptosis, and thus obtain a complete gene expression matrix. Genes and biological functions or pathways significantly associated with QKCMP were obtained using differential gene expression analysis, weighted gene co-expression network analysis (WGCNA), and enrichment analyses. Among them, genes significantly related to QKCMP are enriched in biological processes (BP) such as vascular formation and development, as well as the main signaling pathway: PI3K/AKT signaling pathway. The proproliferative and antiapoptotic effects of QKCMP on the HUVECs and the induction of cell cycle were then verified using cell counting kit 8 (CCK-8) and flow cytometry. Finally, it was confirmed that QKCMP promotes angiogenesis and rapid endothelialization by stimulating the PI3K-AKT and Hippo signaling pathways using quantitative real-time PCR (qRT-PCR) and western blot (WB).</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"22 ","pages":"22808000241301180"},"PeriodicalIF":3.1,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142681957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro assessment of <i>Momordica charantia</i>/<i>Hypericum perforatum</i> oils loaded PCL/Collagen fibers: Novel scaffold for tissue engineering.","authors":"Emre Fatih Ediz, Cansu Güneş, Meltem Demirel Kars, Ahmet Avcı","doi":"10.1177/22808000231221067","DOIUrl":"https://doi.org/10.1177/22808000231221067","url":null,"abstract":"<p><p>The research on tissue engineering applications has been progressing to manufacture ideal tissue scaffold biomaterials. In this study, a double-layered electrospun biofiber scaffold biomaterial including Polycaprolactone (PCL)/Collagen (COL) fibrous inner layer and PCL/ <i>Momordica charantia</i> (MC) and <i>Hypericum perforatum (HP)</i> oils fibrous outer layer was developed to manufacture a functional, novel tissue scaffold with the advantageous mechanical and biological properties. The main approach was to combine the natural perspective using medicinal oils with an engineering point of view to fabricate a potential functional scaffold for tissue engineering. Medicinal plants MC and HP are rich in functional oils and incorporation of them in a tissue scaffold will unveil their potential to augment both new tissue formation and wound healing. In this study, a novel double-layered scaffold prototype was fabricated using electrospinning technique with two PCL fiber layers, first is composed of collagen, and second is composed of oils extracted from medicinal plants. Initially, the composition of plant oils was analyzed. Thereafter the biofiber scaffold layers were fabricated and were evaluated in terms of morphology, physicochemistry, thermal and mechanical features, wettability, in vitro bio-degradability. Double-layered scaffold prototype was further analyzed in terms of in vitro biocompatibility and antibacterial effect. The medicinal oils blend provided antioxidant and antibacterial properties to the novel PCL/Oils layer. The results signify that inner PCL/COL layer exhibited advanced biodegradability of 8.5% compared to PCL and enhanced wettability with 11.7° contact angle. Strength of scaffold prototype was 5.98 N/mm² thanks to the elastic PCL fibrous matrix. The double-layered functional biofiber scaffold enabled 92% viability after 72 h contact with fibroblast cells and furthermore provided feasible attachment sites for the cells. The functional scaffold prototype's noteworthy mechanical, chemical, and biological features enable it to be suggested as a different novel biomaterial with the potential to be utilized in tissue engineering applications.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"22 ","pages":"22808000231221067"},"PeriodicalIF":2.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139466654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}