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Enzymatic hydrolysis and simultaneous saccharification and fermentation of soybean processing intermediates for the production of ethanol and concentration of protein and lipids. 对大豆加工中间产物进行酶水解、同步糖化和发酵,以生产乙醇并浓缩蛋白质和脂质。
ISRN Microbiology Pub Date : 2012-10-17 Print Date: 2012-01-01 DOI: 10.5402/2012/278092
Craig C Long, William Gibbons
{"title":"Enzymatic hydrolysis and simultaneous saccharification and fermentation of soybean processing intermediates for the production of ethanol and concentration of protein and lipids.","authors":"Craig C Long, William Gibbons","doi":"10.5402/2012/278092","DOIUrl":"10.5402/2012/278092","url":null,"abstract":"<p><p>Carbohydrates in soybeans are generally undesirable due to their low digestibility and because they \"dilute\" more valuable components (proteins, lipids). To remove these carbohydrates and raise the titer of more valuable components, ethanol production was investigated. Commercial enzymes (Novozyme cellulase, β -glucosidase, and pectinase) were added to ground soybeans (SB), soybean meal (SBM), soybean hulls (SH), and soybean white flakes (WF) at a 10% solids loading rate to quantify hydrolyzed glucan. Saccharification resulted in glucan reductions of 28%, 45%, 76%, and 80% (SBM, SB, SH, WF, resp.). Simultaneous saccharification and fermentation (SSF) trials were conducted at 5%, 10%, 15%, and 20% solids loading with Saccharomyces cerevisiae NRRL Y-2034 and Scheffersomyces stipitis NRRL Y-7124, with protein, fiber, and lipids analyzed at SSF 10% solids and saccharification trials. S. cerevisiae and S. stipitis produced ~3-12.5 g/L ethanol and ~2.5-8.6 g/L ethanol, respectively, on SB, SBM, and WF over all solid loading rates. SH resulted in higher ethanol titers for both S. cerevisiae (~9-23 g/L) and S. stipitis (~9.5-14.5 g/L). Protein concentrations decreased by 2.5-10% for the SB, SBM, and WF, but increased by 53%-55% in SH. Oil concentrations increased by ~50% for SB; by ~500%-1300% for the others.</p>","PeriodicalId":14849,"journal":{"name":"ISRN Microbiology","volume":"2012 ","pages":"278092"},"PeriodicalIF":0.0,"publicationDate":"2012-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3671703/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31503910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Use of probiotics in aquaculture. 益生菌在水产养殖中的应用。
ISRN Microbiology Pub Date : 2012-10-16 Print Date: 2012-01-01 DOI: 10.5402/2012/916845
Patricia Martínez Cruz, Ana L Ibáñez, Oscar A Monroy Hermosillo, Hugo C Ramírez Saad
{"title":"Use of probiotics in aquaculture.","authors":"Patricia Martínez Cruz,&nbsp;Ana L Ibáñez,&nbsp;Oscar A Monroy Hermosillo,&nbsp;Hugo C Ramírez Saad","doi":"10.5402/2012/916845","DOIUrl":"https://doi.org/10.5402/2012/916845","url":null,"abstract":"<p><p>The growth of aquaculture as an industry has accelerated over the past decades; this has resulted in environmental damages and low productivity of various crops. The need for increased disease resistance, growth of aquatic organisms, and feed efficiency has brought about the use of probiotics in aquaculture practices. The first application of probiotics occurred in 1986, to test their ability to increase growth of hydrobionts (organisms that live in water). Later, probiotics were used to improve water quality and control of bacterial infections. Nowadays, there is documented evidence that probiotics can improve the digestibility of nutrients, increase tolerance to stress, and encourage reproduction. Currently, there are commercial probiotic products prepared from various bacterial species such as Bacillus sp., Lactobacillus sp., Enterococcus sp., Carnobacterium sp., and the yeast Saccharomyces cerevisiae among others, and their use is regulated by careful management recommendations. The present paper shows the current knowledge of the use of probiotics in aquaculture, its antecedents, and safety measures to be carried out and discusses the prospects for study in this field.</p>","PeriodicalId":14849,"journal":{"name":"ISRN Microbiology","volume":"2012 ","pages":"916845"},"PeriodicalIF":0.0,"publicationDate":"2012-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5402/2012/916845","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31595112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 876
Antibiotic Resistance Patterns of Escherichia coli Isolates from Hospitals in Kumasi, Ghana. 加纳库马西医院分离的大肠杆菌抗生素耐药模式
ISRN Microbiology Pub Date : 2012-10-14 Print Date: 2012-01-01 DOI: 10.5402/2012/658470
Duredoh Freeman George, Stephen Yao Gbedema, Christian Agyare, Francis Adu, Vivian Etsiapa Boamah, Adelaide Ama Tawiah, Sixtus Bieranye Bayaa Martin Saana
{"title":"Antibiotic Resistance Patterns of Escherichia coli Isolates from Hospitals in Kumasi, Ghana.","authors":"Duredoh Freeman George,&nbsp;Stephen Yao Gbedema,&nbsp;Christian Agyare,&nbsp;Francis Adu,&nbsp;Vivian Etsiapa Boamah,&nbsp;Adelaide Ama Tawiah,&nbsp;Sixtus Bieranye Bayaa Martin Saana","doi":"10.5402/2012/658470","DOIUrl":"https://doi.org/10.5402/2012/658470","url":null,"abstract":"<p><p>Nosocomial infections are infections acquired by a patient as a result of treatment in a hospital or healthcare service providing center and symptoms occurs within a short period of hospitalization. The study was to determine the antibiotic resistance patterns of Escherichia coli isolated from Kumasi-South, Tafo and Suntreso Hospitals, Kumasi, Ghana. Total of 600 swabs samples from the hospitals were collected between January and June, 2010. The isolates were identified using morphological and biochemical means. A total of 97 E. coli isolates were obtained from the hospitals. Beds in hospital wards had the highest number of E. coli strains (53.6%), followed by floors (20.6%) while drainages had the least isolates (3.1%). Majority of the E. coli isolates (90.7%) exhibited resistance to ampicillin while 6.2 and 3.1% showed intermediate and sensitive respectively. Co-trimoxazole, 78.4% of the isolates were resistant while 9.3 and 12.4% exhibited intermediate and sensitive responses respectively. E. coli isolates (28.6 to 46.4%) were resistant to gentamicin, ciprofloxacin and ceftriaxone while 14.4 to 47.4% gave intermediate responses. Most isolates (80.4%) exhibited multi-drug resistance. There is a need to observe proper personal hygiene, use of effective disinfectants and proper disposal of contaminated/pathogenic materials in these hospitals to control nosocomial infections.</p>","PeriodicalId":14849,"journal":{"name":"ISRN Microbiology","volume":"2012 ","pages":"658470"},"PeriodicalIF":0.0,"publicationDate":"2012-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5402/2012/658470","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31503915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 31
Essential Oils of Plants as Biocides against Microorganisms Isolated from Cuban and Argentine Documentary Heritage. 从古巴和阿根廷文献遗产中分离的植物精油作为微生物的杀菌剂。
ISRN Microbiology Pub Date : 2012-10-14 Print Date: 2012-01-01 DOI: 10.5402/2012/826786
Sofía Borrego, Oderlaise Valdés, Isbel Vivar, Paola Lavin, Patricia Guiamet, Patricia Battistoni, Sandra Gómez de Saravia, Pedro Borges
{"title":"Essential Oils of Plants as Biocides against Microorganisms Isolated from Cuban and Argentine Documentary Heritage.","authors":"Sofía Borrego,&nbsp;Oderlaise Valdés,&nbsp;Isbel Vivar,&nbsp;Paola Lavin,&nbsp;Patricia Guiamet,&nbsp;Patricia Battistoni,&nbsp;Sandra Gómez de Saravia,&nbsp;Pedro Borges","doi":"10.5402/2012/826786","DOIUrl":"https://doi.org/10.5402/2012/826786","url":null,"abstract":"<p><p>Natural products obtained from plants with biocidal activity represent an alternative and useful source in the control of biodeterioration of documentary heritage, without negative environmental and human impacts. In this work, we studied the antimicrobial activity of seven essential oils against microorganisms associated with the biodeterioration of documentary heritage. The essential oils were obtained by steam distillation. The antimicrobial activity was analyzed using the agar diffusion method against 4 strains of fungi and 6 bacterial strains isolated from repositories air and documents of the National Archive of the Republic of Cuba and the Historical Archive of the Museum of La Plata, Argentina. Anise and garlic oils showed the best antifungal activity at all concentrations studied, while oregano oil not only was effective against fungi tested but also prevented sporulation of them all. Orange sweet and laurel oils were ineffective against fungi. Clove, garlic, and oregano oils showed the highest antibacterial activity at 25% against Enterobacter agglomerans and Streptomyces sp., while only clove and oregano oils were effective against Bacillus sp. at all concentrations studied. This study has an important implication for the possible use of the natural products from plants in the control of biodeterioration of documentary heritage.</p>","PeriodicalId":14849,"journal":{"name":"ISRN Microbiology","volume":"2012 ","pages":"826786"},"PeriodicalIF":0.0,"publicationDate":"2012-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5402/2012/826786","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31595110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 84
Candida albicans: A Model Organism for Studying Fungal Pathogens. 白色念珠菌:研究真菌病原体的模式生物。
ISRN Microbiology Pub Date : 2012-09-29 Print Date: 2012-01-01 DOI: 10.5402/2012/538694
M Anaul Kabir, Mohammad Asif Hussain, Zulfiqar Ahmad
{"title":"Candida albicans: A Model Organism for Studying Fungal Pathogens.","authors":"M Anaul Kabir,&nbsp;Mohammad Asif Hussain,&nbsp;Zulfiqar Ahmad","doi":"10.5402/2012/538694","DOIUrl":"https://doi.org/10.5402/2012/538694","url":null,"abstract":"<p><p>Candida albicans is an opportunistic human fungal pathogen that causes candidiasis. As healthcare has been improved worldwide, the number of immunocompromised patients has been increased to a greater extent and they are highly susceptible to various pathogenic microbes and C. albicans has been prominent among the fungal pathogens. The complete genome sequence of this pathogen is now available and has been extremely useful for the identification of repertoire of genes present in this pathogen. The major challenge is now to assign the functions to these genes of which 13% are specific to C. albicans. Due to its close relationship with yeast Saccharomyces cerevisiae, an edge over other fungal pathogens because most of the technologies can be directly transferred to C. albicans from S. cerevisiae and it is amenable to mutation, gene disruption, and transformation. The last two decades have witnessed enormous amount of research activities on this pathogen that leads to the understanding of host-parasite interaction, infections, and disease propagation. Clearly, C. albicans has emerged as a model organism for studying fungal pathogens along with other two fungi Aspergillus fumigatus and Cryptococcus neoformans. Understanding its complete life style of C. albicans will undoubtedly be useful for developing potential antifungal drugs and tackling Candida infections. This will also shed light on the functioning of other fungal pathogens.</p>","PeriodicalId":14849,"journal":{"name":"ISRN Microbiology","volume":"2012 ","pages":"538694"},"PeriodicalIF":0.0,"publicationDate":"2012-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5402/2012/538694","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31503914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 136
Integument Mycobiota of Wild European Hedgehogs (Erinaceus europaeus) from Catalonia, Spain. 西班牙加泰隆尼亚野生欧洲刺猬(Erinaceus europaeus)的被皮菌群。
ISRN Microbiology Pub Date : 2012-09-25 Print Date: 2012-01-01 DOI: 10.5402/2012/659754
R A Molina-López, C Adelantado, E L Arosemena, E Obón, L Darwich, M A Calvo
{"title":"Integument Mycobiota of Wild European Hedgehogs (Erinaceus europaeus) from Catalonia, Spain.","authors":"R A Molina-López,&nbsp;C Adelantado,&nbsp;E L Arosemena,&nbsp;E Obón,&nbsp;L Darwich,&nbsp;M A Calvo","doi":"10.5402/2012/659754","DOIUrl":"https://doi.org/10.5402/2012/659754","url":null,"abstract":"<p><p>There are some reports about the risk of manipulating wild hedgehogs since they can be reservoirs of potential zoonotic agents like dermatophytes. The aim of this study was to describe the integument mycobiota, with special attention to dermatophytes of wild European hedgehogs. Samples from spines and fur were cultured separately in Sabouraud dextrose agar (SDA) with antibiotic and dermatophyte test medium (DTM) plates. Nineteen different fungal genera were isolated from 91 cultures of 102 hedgehogs. The most prevalent genera were Cladosporium (79.1%), Penicillium (74.7%), Alternaria (64.8%), and Rhizopus (63.7%). A lower prevalence of Aspergillus (P = 0,035; χ (2) = 8,633) and Arthrinium (P = 0,043; χ (2) = 8,173) was isolated during the spring time and higher frequencies of Fusarium (P = 0,015; χ (2) = 10,533) during the autumn. The prevalence of Acremonium was significantly higher in young animals (70%, 26/37) than in adults (30%, 11/37) (P = 0,019; χ (2) = 5,915). Moreover, the majority of the saprophytic species that grew at the SDA culture were also detected at the DTM. Finally, no cases of ringworm were diagnosed and no dermatophytes spp. were isolated. Concluding, this study provides the first description of fungal mycobiota of the integument of wild European hedgehogs in Spain, showing a large number of saprophytic species and the absence of dermatophytes.</p>","PeriodicalId":14849,"journal":{"name":"ISRN Microbiology","volume":"2012 ","pages":"659754"},"PeriodicalIF":0.0,"publicationDate":"2012-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5402/2012/659754","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31503916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Clinical and Epidemiologic Features of Diarrheal Disease due to Aeromonas hydrophila and Plesiomonas shigelloides Infections Compared with Those due to Vibrio cholerae Non-O1 and Vibrio parahaemolyticus in Bangladesh. 孟加拉国嗜水气单胞菌和志贺氏单胞菌感染与非o1型霍乱弧菌和副溶血性弧菌感染腹泻的临床和流行病学特征
ISRN Microbiology Pub Date : 2012-09-24 Print Date: 2012-01-01 DOI: 10.5402/2012/654819
Erik H Klontz, Abu S G Faruque, Sumon K Das, Mohammed A Malek, Zhahirul Islam, Stephen P Luby, Karl C Klontz
{"title":"Clinical and Epidemiologic Features of Diarrheal Disease due to Aeromonas hydrophila and Plesiomonas shigelloides Infections Compared with Those due to Vibrio cholerae Non-O1 and Vibrio parahaemolyticus in Bangladesh.","authors":"Erik H Klontz,&nbsp;Abu S G Faruque,&nbsp;Sumon K Das,&nbsp;Mohammed A Malek,&nbsp;Zhahirul Islam,&nbsp;Stephen P Luby,&nbsp;Karl C Klontz","doi":"10.5402/2012/654819","DOIUrl":"https://doi.org/10.5402/2012/654819","url":null,"abstract":"<p><p>Using data from the International Centre for Diarrhoeal Disease Research, Bangladesh (icddr,b) from 1996 to 2001, we compared the clinical features of diarrhea in patients with stool specimens yielding only A. hydrophila (189 patients; 1.4% of 13,970 patients screened) or P. shigelloides (253 patients) compared to patients with sole V. cholerae non-O1 infection (99 patients) or V. parahaemolyticus infection (126 patients). Patients exhibited similar frequencies of fever (temperature >37.8°C), stools characterized as watery, and stools containing visible mucus. Dehydration was observed more often among patients with V. parahaemolyticus or V. cholerae non-O1 infection. Compared to patients infected with V. parahaemolyticus, those with A. hydrophila, P. shigelloides, or V. cholerae non-O1 infection were less likely to report visible blood in the stool and, on microscopic examination, less likely to exhibit stool red blood cell and white blood cell counts exceeding 20 cells per high power field. The proportion of patients reporting subjective cure at the time of discharge was significantly smaller for those infected with V. parahaemolyticus. These findings suggest that A. hydrophila and P. shigelloides produce diarrheal disease that is less severe than that resulting from infection with V. cholerae non-O1 or V. parahaemolyticus.</p>","PeriodicalId":14849,"journal":{"name":"ISRN Microbiology","volume":"2012 ","pages":"654819"},"PeriodicalIF":0.0,"publicationDate":"2012-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5402/2012/654819","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31503912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 17
Pullulan Production by Aureobasidium pullulans ATCC 201253 Cells Adsorbed onto Cellulose Anion and Cation Exchangers. 吸附在纤维素阴阳离子交换器上的 Aureobasidium pullulans ATCC 201253 细胞生产普鲁兰。
ISRN Microbiology Pub Date : 2012-09-23 Print Date: 2012-01-01 DOI: 10.5402/2012/140951
Thomas P West
{"title":"Pullulan Production by Aureobasidium pullulans ATCC 201253 Cells Adsorbed onto Cellulose Anion and Cation Exchangers.","authors":"Thomas P West","doi":"10.5402/2012/140951","DOIUrl":"10.5402/2012/140951","url":null,"abstract":"<p><p>The anion exchanger phosphocellulose and the cation exchanger triethylaminoethyl cellulose were used to immobilize cells of the fungus Aureobasidium pullulans ATCC 201253 and the adsorbed cells were subsequently investigated for their ability to produce the polysaccharide pullulan using batch fermentation. The cells adsorbed on the triethylaminoethyl cellulose at pH 7.5 produced higher pullulan levels than those cells immobilized on phosphocellulose at pH 4.0 for 2 cycles of 168 h at 30 °C. Relative to the initial cycle of 168 h, pullulan production by the cells immobilized on the triethylaminoethyl cellulose decreased slightly after 168 h of the second production cycle while pullulan production by the phosphocellulose-immobilized cells remained about the same after 168 h of the second production cycle.</p>","PeriodicalId":14849,"journal":{"name":"ISRN Microbiology","volume":"2012 ","pages":"140951"},"PeriodicalIF":0.0,"publicationDate":"2012-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3664200/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31502970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Important Roles of Cellular MicroRNA miR-155 in Leukemogenesis by Human T-Cell Leukemia Virus Type 1 Infection. 细胞MicroRNA miR-155在人t细胞白血病病毒1型感染白血病发生中的重要作用
ISRN Microbiology Pub Date : 2012-09-18 Print Date: 2012-01-01 DOI: 10.5402/2012/978607
Mariko Tomita
{"title":"Important Roles of Cellular MicroRNA miR-155 in Leukemogenesis by Human T-Cell Leukemia Virus Type 1 Infection.","authors":"Mariko Tomita","doi":"10.5402/2012/978607","DOIUrl":"https://doi.org/10.5402/2012/978607","url":null,"abstract":"<p><p>Human T-cell leukemia virus type 1 (HTLV-1) is the pathogen that causes the aggressive and lethal malignancy of CD4+ T-lymphocytes called adult T-cell leukemia/lymphoma (ATLL). MicroRNAs (miRNAs), a class of short, noncoding RNAs, regulate gene expression by targeting mRNAs for translational repression or cleavage. miRNAs are involved in many aspects of cell biology linked with formation of several cancer phenotypes. However, the relation between miRNAs and pathologic implication in ATLL is not well elucidated. Here, we evaluated the roles of cellular miRNAs in ATLL caused by HTLV-1. We found that the expression of miR-155 was increased in HTLV-1-positive T-cell lines. miR-155 expression was enhanced by Tax and binding of transcription factors, NF- κ B and AP-1, on the transcription binding sites of miR-155 gene promoter region is important to increase the expression of miR-155 by Tax. Transfection of anti-miR-155 inhibitor, which inhibits the function of miR-155, inhibited the growth of HTLV-1-positive T-cell lines. On the other hand, the growth of HTLV-1-negative T-cell lines was not changed by transfection of anti-miR-155. Forced expression of miR-155 enhanced the growth of HTLV-1-positive T-cell lines. These findings indicate that targeting the functions of miRNAs is a novel approach to the prevention or treatment of ATLL.</p>","PeriodicalId":14849,"journal":{"name":"ISRN Microbiology","volume":"2012 ","pages":"978607"},"PeriodicalIF":0.0,"publicationDate":"2012-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5402/2012/978607","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31595113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 23
Comparison of an automated system with conventional identification and antimicrobial susceptibility testing. 自动系统与常规鉴定及药敏试验的比较。
ISRN Microbiology Pub Date : 2012-09-16 Print Date: 2012-01-01 DOI: 10.5402/2012/107203
Shalini Duggal, Rajni Gaind, Neha Tandon, Manorama Deb, Tulsi Das Chugh
{"title":"Comparison of an automated system with conventional identification and antimicrobial susceptibility testing.","authors":"Shalini Duggal,&nbsp;Rajni Gaind,&nbsp;Neha Tandon,&nbsp;Manorama Deb,&nbsp;Tulsi Das Chugh","doi":"10.5402/2012/107203","DOIUrl":"https://doi.org/10.5402/2012/107203","url":null,"abstract":"<p><p>The present study was designed to compare a fully automated identification/antibiotic susceptibility testing (AST) system BD Phoenix (BD) for its efficacy in rapid and accurate identification and AST with conventional manual methods and to determine if the errors reported in AST, such as the (very major errors) VME (false susceptibility), (major errors) ME (false resistance), and (minor errors) MiE (intermediate category interpretation) were within the range certified by FDA. Identification and antimicrobial susceptibility test results of eighty-five clinical isolates including both gram-positive and negative were compared on Phoenix considering the results obtained from conventional manual methods of identification and disc diffusion testing of antibiotics as standards for comparison. Phoenix performed favorably well. There was 100% concordance in identification for gram-negative isolates and 94.83% for gram-positive isolates. In seven cases, Phoenix proved better than conventional identification. For antibiotic results, categorical agreement was 98.02% for gram-positive and 95.7% for gram-negative isolates. VME was 0.33%, ME 0.66%, MiE 0.99% for gram-positive isolates and 1.23% VME, 1.23% ME, and 1.85% MiE for gram-negative isolates. Therefore, this automated system can be used as a tool to facilitate early identification and susceptibility pattern of aerobic bacteria in routine microbiology laboratories.</p>","PeriodicalId":14849,"journal":{"name":"ISRN Microbiology","volume":"2012 ","pages":"107203"},"PeriodicalIF":0.0,"publicationDate":"2012-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5402/2012/107203","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31502969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 22
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