Pullulan Production by Aureobasidium pullulans ATCC 201253 Cells Adsorbed onto Cellulose Anion and Cation Exchangers.

ISRN Microbiology Pub Date : 2012-09-23 Print Date: 2012-01-01 DOI:10.5402/2012/140951
Thomas P West
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Abstract

The anion exchanger phosphocellulose and the cation exchanger triethylaminoethyl cellulose were used to immobilize cells of the fungus Aureobasidium pullulans ATCC 201253 and the adsorbed cells were subsequently investigated for their ability to produce the polysaccharide pullulan using batch fermentation. The cells adsorbed on the triethylaminoethyl cellulose at pH 7.5 produced higher pullulan levels than those cells immobilized on phosphocellulose at pH 4.0 for 2 cycles of 168 h at 30 °C. Relative to the initial cycle of 168 h, pullulan production by the cells immobilized on the triethylaminoethyl cellulose decreased slightly after 168 h of the second production cycle while pullulan production by the phosphocellulose-immobilized cells remained about the same after 168 h of the second production cycle.

Abstract Image

Abstract Image

吸附在纤维素阴阳离子交换器上的 Aureobasidium pullulans ATCC 201253 细胞生产普鲁兰。
阴离子交换剂磷纤维素和阳离子交换剂三乙胺基乙基纤维素被用来固定真菌 Aureobasidium pullulans ATCC 201253 的细胞,随后研究了被吸附细胞利用批量发酵生产多糖拉普兰的能力。与固定在 pH 值为 4.0 的磷纤维素上的细胞相比,吸附在 pH 值为 7.5 的三乙胺基乙基纤维素上的细胞在 30 °C、168 小时的两个循环中产生的拉胶量更高。与最初的 168 小时周期相比,固定在三乙氨基乙基纤维素上的细胞在第二个生产周期 168 小时后的拉聚糖产量略有下降,而固定在磷纤维素上的细胞在第二个生产周期 168 小时后的拉聚糖产量基本保持不变。
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