ISRN Microbiology最新文献_第6页

Use of bacterial artificial chromosomes in baculovirus research and recombinant protein expression: current trends and future perspectives. 细菌人工染色体在杆状病毒研究和重组蛋白表达中的应用:当前趋势和未来展望。

ISRN Microbiology Pub Date : 2012-09-12 Print Date: 2012-01-01 DOI: 10.5402/2012/628797

Polly Roy, Rob Noad

引用次数: 9

Thermostable and Alkalistable Xylanases Produced by the Thermophilic Bacterium Anoxybacillus flavithermus TWXYL3. 嗜热细菌黄热无氧杆菌TWXYL3产生的耐热和可碱性木聚糖酶。

ISRN Microbiology Pub Date : 2012-09-05 Print Date: 2012-01-01 DOI: 10.5402/2012/517524

Joshua T Ellis, Timothy S Magnuson

{"title":"Thermostable and Alkalistable Xylanases Produced by the Thermophilic Bacterium Anoxybacillus flavithermus TWXYL3.","authors":"Joshua T Ellis, Timothy S Magnuson","doi":"10.5402/2012/517524","DOIUrl":"https://doi.org/10.5402/2012/517524","url":null,"abstract":"With the rising cost and finite supply of fossil energy, there is an increasing economic incentive for the development of clean, efficient, and renewable domestic energy. The activities of microorganisms offer the potential conversion of lignocellulosic materials into fermentable sugars, usable for downstream fermentation processes. Strain TWXYL3, a thermophilic facultative anaerobe, was discovered in the Alvord Basin hydrothermal system in Oregon, USA. Phylogenetic analysis of strain TWXYL3 showed it to be 99% similar to the 16S rRNA gene of Anoxybacillus flavithermus WL (FJ950739). A. flavithermus TWXYL3 was shown to secrete a large multisubunit thermostable xylanase complex into the growth medium. Xylanase induction was achieved by resuspending the isolate in a selective xylan-containing medium. Extracellular xylanase activity showed a temperature optimum of 65°C and retained thermostability up to 85°C. Extracellular xylanase activity showed a bimodal pH optimum, with maxima at pH 6 and pH 8. Electrophoretic analysis of the extracellular xylanase shows 5 distinct proteins with xylanase activity. Strain TWXYL3 is the first xylanolytic isolate obtained from the Alvord Basin hydrothermal system and represents a new model system for development of processes where lignocellulosics are converted to biofuel precursors.","PeriodicalId":14849,"journal":{"name":"ISRN Microbiology","volume":"2012 ","pages":"517524"},"PeriodicalIF":0.0,"publicationDate":"2012-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5402/2012/517524","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31503911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 33

The main Aeromonas pathogenic factors. 气单胞菌的主要致病因素。

ISRN Microbiology Pub Date : 2012-09-04 Print Date: 2012-01-01 DOI: 10.5402/2012/256261

J M Tomás

引用次数: 247

Comparison of the Serological Reactivity of Lipopolysaccharides from Japanese and Western Strains of Helicobacter pylori to Sera from H. pylori-Positive Humans. 幽门螺杆菌日本株与西方株脂多糖对幽门螺杆菌阳性者血清的血清学反应性比较。

ISRN Microbiology Pub Date : 2012-07-26 Print Date: 2012-01-01 DOI: 10.5402/2012/162816

Ken-Ichi Amano, Shin-Ichi Yokota, Mario A Monteiro

引用次数: 2

Parasitic prevalence in a suburban school of famaillá, tucumán, Argentina. 阿根廷famaill<e:1> (tucumán)郊区一所学校的寄生虫流行情况。

ISRN Microbiology Pub Date : 2012-06-26 Print Date: 2012-01-01 DOI: 10.5402/2012/560376

Julián Dib, Juana Oquilla, Silvia G Lazarte, Silvia N Gonzalez

引用次数: 14

Assessment of some inflammatory biomarkers as predictors of outcome of acute respiratory failure on top of chronic obstructive pulmonary disease and evaluation of the role of bacteria. 评估作为慢性阻塞性肺病急性呼吸衰竭预后指标的一些炎症生物标志物，并评估细菌的作用。

ISRN Microbiology Pub Date : 2012-06-21 Print Date: 2012-01-01 DOI: 10.5402/2012/240841

Hanaa Ahmed Shafiek, Nashwa Hassan Abd-Elwahab, Manal Mohammad Baddour, Mohamed Mabrouk El-Hoffy, Akram Abd-Elmoneim Degady, Yehia Mohamed Khalil

{"title":"Assessment of some inflammatory biomarkers as predictors of outcome of acute respiratory failure on top of chronic obstructive pulmonary disease and evaluation of the role of bacteria.","authors":"Hanaa Ahmed Shafiek, Nashwa Hassan Abd-Elwahab, Manal Mohammad Baddour, Mohamed Mabrouk El-Hoffy, Akram Abd-Elmoneim Degady, Yehia Mohamed Khalil","doi":"10.5402/2012/240841","DOIUrl":"10.5402/2012/240841","url":null,"abstract":"Objective. To study the value of the inflammatory markers (interleukin-6 (IL-6), interleukin-8 (IL-8), and C-reactive protein (CRP)) in predicting the outcome of noninvasive ventilation (NIV) in the management of acute respiratory failure (ARF) on top of chronic obstructive pulmonary disease (COPD) and the role of bacteria in the systemic inflammation. Methods. Thirty three patients were subjected to standard treatment plus NIV, and accordingly, they were classified into responders and nonresponders. Serum samples were collected for IL-6, IL-8, and CRP analysis. Sputum samples were taken for microbiological evaluation. Results. A wide spectrum of bacteria was revealed; Gram-negative and atypical bacteria were the most common (31% and 28% resp.; single or copathogen). IL-8 and dyspnea grade was significantly higher in the non-responder group (P = 0.01 and 0.023 resp.). IL-6 correlated positivity with the presence of infection and type of pathogen (P = 0.038 and 0.034 resp.). Gram-negative bacteria were associated with higher significant IL-6 in comparison between others (196.4 ± 239.1 pg/dL; P = 0.011) but insignificantly affected NIV outcome (P > 0.05). Conclusions. High systemic inflammation could predict failure of NIV. G-ve bacteria correlated with high IL-6 but did not affect the response to NIV.","PeriodicalId":14849,"journal":{"name":"ISRN Microbiology","volume":"2012 ","pages":"240841"},"PeriodicalIF":0.0,"publicationDate":"2012-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3658585/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31470253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of a Methanogenic Community within an Algal Fed Anaerobic Digester. 藻类厌氧消化池内产甲烷菌群的特征。

ISRN Microbiology Pub Date : 2012-06-21 Print Date: 2012-01-01 DOI: 10.5402/2012/753892

Joshua T Ellis, Cody Tramp, Ronald C Sims, Charles D Miller

{"title":"Characterization of a Methanogenic Community within an Algal Fed Anaerobic Digester.","authors":"Joshua T Ellis, Cody Tramp, Ronald C Sims, Charles D Miller","doi":"10.5402/2012/753892","DOIUrl":"https://doi.org/10.5402/2012/753892","url":null,"abstract":"The microbial diversity and metabolic potential of a methanogenic consortium residing in a 3785-liter anaerobic digester, fed with wastewater algae, was analyzed using 454 pyrosequencing technology. DNA was extracted from anaerobic sludge material and used in metagenomic analysis through PCR amplification of the methyl-coenzyme M reductase α subunit (mcrA) gene using primer sets ML, MCR, and ME. The majority of annotated mcrA sequences were assigned taxonomically to the genera Methanosaeta in the order Methanosarcinales. Methanogens from the genus Methanosaeta are obligate acetotrophs, suggesting this genus plays a dominant role in methane production from the analyzed fermentation sample. Numerous analyzed sequences within the algae fed anaerobic digester were unclassified and could not be assigned taxonomically. Relative amplicon frequencies were determined for each primer set to determine the utility of each in pyrosequencing. Primer sets ML and MCR performed better quantitatively (representing the large majority of analyzed sequences) than primer set ME. However, each of these primer sets was shown to provide a quantitatively unique community structure, and thus they are of equal importance in mcrA metagenomic analysis.","PeriodicalId":14849,"journal":{"name":"ISRN Microbiology","volume":"2012 ","pages":"753892"},"PeriodicalIF":0.0,"publicationDate":"2012-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5402/2012/753892","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31471315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 34

Genetic Diversity of Colletotrichum spp. an Endophytic Fungi in a Medicinal Plant, Brazilian Pepper Tree. 药用植物巴西胡椒树中内生真菌 Colletotrichum spp.

ISRN Microbiology Pub Date : 2012-05-30 Print Date: 2012-01-01 DOI: 10.5402/2012/215716

J S Lima, J G Figueiredo, R G Gomes, D Stringari, E H Goulin, D Adamoski, V Kava-Cordeiro, L V Galli-Terasawa, C Glienke

{"title":"Genetic Diversity of Colletotrichum spp. an Endophytic Fungi in a Medicinal Plant, Brazilian Pepper Tree.","authors":"J S Lima, J G Figueiredo, R G Gomes, D Stringari, E H Goulin, D Adamoski, V Kava-Cordeiro, L V Galli-Terasawa, C Glienke","doi":"10.5402/2012/215716","DOIUrl":"10.5402/2012/215716","url":null,"abstract":"In this study, we reported thirty-nine endophytic fungi identified as Colletotrichum spp. associated with Brazilian pepper tree or aroeira (Schinus terebinthifolius Raddi. Anacardiaceae) in Paraná state, Brazil. These endophytes were identified by morphological and molecular methods, using PCR taxon-specific with CaInt/ITS4, CgInt/ITS4, and Col1/ITS4 primers, which amplify specific bands in C. acutatum, C. gloeosporioides lato sensu, and Colletotrichum boninensis, respectively, and by DNA sequence analysis of the nrDNA internal transcribed spacer region (ITS1, 5.8S, ITS2). We also assayed the presence of dsRNA particles in Colletotrichum spp. isolates. Combining both morphological characters and molecular data, we identified the species C. gloeosporioides, C. boninense, and C. simmondsii. However, we found a high genetic variability intraspecific in C. gloeosporioides which suggests the existence of several other species. Bands of double-stranded RNA (dsRNA) were detected in three of thirty-nine isolates. Identity of these bands was confirmed by RNAse, DNAse, and S1 nuclease treatments for the isolates LGMF633, LGMF726, and LGMF729. This is the first study reporting these particles of dsRNA in C. gloeosporioides.","PeriodicalId":14849,"journal":{"name":"ISRN Microbiology","volume":"2012 ","pages":"215716"},"PeriodicalIF":0.0,"publicationDate":"2012-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3658576/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31470252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Escherichia coli GcvB sRNA Uses Genetic Redundancy to Control cycA Expression. 大肠杆菌GcvB sRNA利用遗传冗余控制cycA表达。

ISRN Microbiology Pub Date : 2012-05-28 Print Date: 2012-01-01 DOI: 10.5402/2012/636273

Lorraine T Stauffer, George V Stauffer

引用次数: 10

Genetic Determinants of Methicillin Resistance and Virulence among Staphylococcus aureus Isolates Recovered from Clinical and Surveillance Cultures in a Brazilian Teaching Hospital. 巴西一家教学医院临床和监测培养金黄色葡萄球菌耐药性和毒力的遗传决定因素

ISRN Microbiology Pub Date : 2012-05-17 Print Date: 2012-01-01 DOI: 10.5402/2012/975143

Marcus Vinicius Pimenta Rodrigues, Carlos Magno Castelo Branco Fortaleza, Camila Sena Martins Souza, Natalia Bibiana Teixeira, Maria de Lourdes Ribeiro de Souza da Cunha

{"title":"Genetic Determinants of Methicillin Resistance and Virulence among Staphylococcus aureus Isolates Recovered from Clinical and Surveillance Cultures in a Brazilian Teaching Hospital.","authors":"Marcus Vinicius Pimenta Rodrigues, Carlos Magno Castelo Branco Fortaleza, Camila Sena Martins Souza, Natalia Bibiana Teixeira, Maria de Lourdes Ribeiro de Souza da Cunha","doi":"10.5402/2012/975143","DOIUrl":"https://doi.org/10.5402/2012/975143","url":null,"abstract":"Aims. To quantify the presence of SCCmec types and virulence genes among Staphylococcus aureus colonizing and infecting patients from a teaching hospital. Methods. We analyzed 225 and 84 S. aureus isolates recovered from surveillance and clinical cultures, respectively. Strains were studied for the presence and type of SCCmec, as well as for several virulence genes. Univariate and multivariable analysis were performed in order to identify predictors of invasiveness (defined as isolation from clinical cultures). Results. The presence of SCCmec types III (OR, 2.19, 95% CI, 1.08-4.45) and IV (OR, 5.28 95% CI, 1.35-20.63) and of genes coding for exfoliative toxin B (etb, OR, 6.38, 95% CI, 1.48-27.46) and Panton-Valentine leukocidin (pvl, OR, 2.38, 95% CI, 1.16-4.86) was independently associated with invasiveness. Conclusions. SCCmec types III and IV and virulence genes are associated with greater invasiveness of S. aureus. Patients colonized with methicillin-resistant S. aureus, as well as with strains harboring etb or pvl, may be prone to develop invasive disease. Infection-preventing strategies should be more intensively applied to this group.","PeriodicalId":14849,"journal":{"name":"ISRN Microbiology","volume":"2012 ","pages":"975143"},"PeriodicalIF":0.0,"publicationDate":"2012-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5402/2012/975143","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31471827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3