ISRN Microbiology最新文献_第9页

Glucan Biosynthesis Protein G Is a Suitable Reference Gene in Escherichia coli K-12. 葡聚糖生物合成蛋白G是大肠杆菌K-12的合适内参基因。

ISRN Microbiology Pub Date : 2011-11-23 Print Date: 2011-01-01 DOI: 10.5402/2011/469053

Sean S J Heng, Oliver Y W Chan, Bryan M H Keng, Maurice H T Ling

{"title":"Glucan Biosynthesis Protein G Is a Suitable Reference Gene in Escherichia coli K-12.","authors":"Sean S J Heng, Oliver Y W Chan, Bryan M H Keng, Maurice H T Ling","doi":"10.5402/2011/469053","DOIUrl":"https://doi.org/10.5402/2011/469053","url":null,"abstract":"The expressions of reference genes used in gene expression studies are assumed to be stable under most circumstances. However, a number of studies had demonstrated that such genes were found to vary under experimental conditions. In addition, genes that are stably expressed in an organ may not be stably expressed in other organs or other organisms, suggesting the need to identify reference genes for each organ and organism. This study aims at identifying stably expressed genes in Escherichia coli. Microarray datasets from E. coli substrain MG1655 and 1 dataset from W3110 were analysed. Coefficient of variance (COV) of was calculated and 10% of the lowest COV from 4631 genes common in the 3 MG1655 sets were analysed using NormFinder. Glucan biosynthesis protein G (mdoG), which is involved in cell wall synthesis, displayed the lowest weighted COV and weighted NormFinder Stability Index for the MG1655 datasets, while also showing to be the most stable in the dataset for substrain W3110, suggesting that mdoG is a suitable reference gene for E. coli K-12. Gene ontology over-representation analysis on the 39 genes suggested an over-representation of cell division, carbohydrate metabolism, and protein synthesis which supports the short generation time of E. coli.","PeriodicalId":14849,"journal":{"name":"ISRN Microbiology","volume":"2011 ","pages":"469053"},"PeriodicalIF":0.0,"publicationDate":"2011-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3658596/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31563731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 29

Activity of Ceftaroline against Aerobic Gram-Positive and Gram-Negative Pathogens: Effect of Test Method Variability. 头孢他林对需氧革兰氏阳性和革兰氏阴性病原体的活性:试验方法可变性的影响。

ISRN Microbiology Pub Date : 2011-11-17 Print Date: 2011-01-01 DOI: 10.5402/2011/787290

Diane M Citron, Yumi A Warren, Kerin L Tyrrell, Ellie J C Goldstein

引用次数: 0

Genetic Diversity and Drug Resistance Mutations in HIV-1 from Untreated Patients in Niamey, Niger. 尼日尔尼亚美未经治疗的HIV-1患者的遗传多样性和耐药突变

ISRN Microbiology Pub Date : 2011-11-03 Print Date: 2011-01-01 DOI: 10.5402/2011/797463

Saïdou Mamadou, Yahayé Hanki, Amadou Roufaï Ali Maazou, Balki Aoula, Sanata Diallo

引用次数: 7

Improved Mannanase Production from Penicillium occitanis by Fed-Batch Fermentation Using Acacia Seeds. 利用金合欢种子进行分批饲喂发酵，提高枕青霉的甘露聚糖酶产量

ISRN Microbiology Pub Date : 2011-10-24 Print Date: 2011-01-01 DOI: 10.5402/2011/938347

Monia Blibech, Raoudha Ellouz Ghorbel, Fatma Chaari, Ilyes Dammak, Fatma Bhiri, Mohamed Neifar, Semia Ellouz Chaabouni

引用次数: 0

Optimization of Chromium Removal by the Indigenous Bacterium Bacillus spp. REP02 Using the Response Surface Methodology. 响应面法优化本土细菌芽孢杆菌REP02对铬的去除效果。

ISRN Microbiology Pub Date : 2011-10-19 Print Date: 2011-01-01 DOI: 10.5402/2011/951694

C K Venil, V Mohan, P Lakshmanaperumalsamy, M B Yerima

引用次数: 19

Toxins and Antibiotic Resistance in Staphylococcus aureus Isolated from a Major Hospital in Lebanon. 从黎巴嫩一家大型医院分离出的金黄色葡萄球菌的毒素和抗生素耐药性。

ISRN Microbiology Pub Date : 2011-09-18 Print Date: 2011-01-01 DOI: 10.5402/2011/812049

Sima Tokajian, Dominik Haddad, Rana Andraos, Fuad Hashwa, George Araj

{"title":"Toxins and Antibiotic Resistance in Staphylococcus aureus Isolated from a Major Hospital in Lebanon.","authors":"Sima Tokajian, Dominik Haddad, Rana Andraos, Fuad Hashwa, George Araj","doi":"10.5402/2011/812049","DOIUrl":"10.5402/2011/812049","url":null,"abstract":"Molecular characterization of Staphylococcus aureus is of both clinical and infection control importance. Virulence determinants using PCR and multiple drug resistance profiles were studied in 130 S. aureus isolates. PCR-RFLP analysis of the 16S-23S DNA spacer region was done to investigate the level of 16S-23S ITS (internal transcribed spacer) polymorphism. Methicillin-resistant S. aureus (MRSA), which represented 72% of the studied isolates, showed multiple drug resistance with 18% being resistant to 10-18 of the drugs used compared to a maximum resistance to 9 antibiotics with the methicillin sensitive S. aureus (MSSA) isolates. Exfoliative toxin A (ETA) was more prevalent than B (ETB) with virulent determinants being additionally detected in multiple drug-resistant isolates. 16S-23S ITS PCR-RFLP combined with sequencing of the primary product was successful in generating molecular fingerprints of S. aureus and could be used for preliminary typing. This is the first study to demonstrate the incidence of virulent genes, ACME, and genetic diversity of S. aureus isolates in Lebanon. The data presented here epitomize a starting point defining the major genetic populations of both MRSA and MSSA in Lebanon and provide a basis for clinical epidemiological studies.","PeriodicalId":14849,"journal":{"name":"ISRN Microbiology","volume":"2011 ","pages":"812049"},"PeriodicalIF":0.0,"publicationDate":"2011-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3658828/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31470898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0