ISRN Microbiology最新文献_第8页

Resistance index of penicillin-resistant bacteria to various physicochemical agents. 青霉素耐药菌对各种理化药剂的耐药指数。

ISRN Microbiology Pub Date : 2012-01-31 Print Date: 2012-01-01 DOI: 10.5402/2012/789474

M Kazemi, R Kasra Kermanshahi, E Heshmat Dehkordi, F Payami, M Behjati

{"title":"Resistance index of penicillin-resistant bacteria to various physicochemical agents.","authors":"M Kazemi, R Kasra Kermanshahi, E Heshmat Dehkordi, F Payami, M Behjati","doi":"10.5402/2012/789474","DOIUrl":"https://doi.org/10.5402/2012/789474","url":null,"abstract":"Widespread use of various antimicrobial agents resulted in the emergence of bacterial resistance. Mechanisms like direct efflux, formation, and sequestration of metals and drugs in complexes and antiporter pumps are some examples. This investigation aims to investigate the resistance pattern of penicillin-resistant bacterial strains to some physicochemical agents. Sensitivity/resistance pattern of common bacterial strains to antimicrobial agents were evaluated by disk diffusion assay. Broth and agar dilution method were used for determination of minimum inhibitory concentration and minimal bactericidal concentration. The impact of UV ray on the bacterial growth under laminar flow hood was measured using photonmeter. Our data demonstrates that the most prevalent metal resistance was against arsenate (95.92%), followed by cadmium (52.04%) and mercury (36.73%). There was significant difference between cetrimide resistances among studied microbial strains especially for P. aeruginosa (P < 0.05). High rate of pathogen resistance to various antibacterial agents in our study supports previously published data. This great rate of bacterial resistance is attributed to the emergence of defense mechanisms developed in pathogens. The higher general bacterial resistance rate among Staphylococcus strains rather than E. coli and P. aeruginosa strains draws attention towards focusing on designing newer therapeutic compounds for Staphylococcus strains.","PeriodicalId":14849,"journal":{"name":"ISRN Microbiology","volume":"2012 ","pages":"789474"},"PeriodicalIF":0.0,"publicationDate":"2012-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5402/2012/789474","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31471824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Degradation of Phenol via Meta Cleavage Pathway by Pseudomonas fluorescens PU1. 荧光假单胞菌PU1通过元裂解途径降解苯酚

ISRN Microbiology Pub Date : 2012-01-23 Print Date: 2012-01-01 DOI: 10.5402/2012/741820

Md Mahiudddin, A N M Fakhruddin, Abdullah-Al-Mahin

{"title":"Degradation of Phenol via Meta Cleavage Pathway by Pseudomonas fluorescens PU1.","authors":"Md Mahiudddin, A N M Fakhruddin, Abdullah-Al-Mahin","doi":"10.5402/2012/741820","DOIUrl":"https://doi.org/10.5402/2012/741820","url":null,"abstract":"Degradation of phenolics by members of soil microflora is an important means by which these substances are removed from the environment thus reducing environmental pollution. Biodegradation by microorganisms offers unique opportunities to destroy or render phenolic compounds. A bacterium, PU1, identified as Pseudomonas fluorescens PU1, was investigated for its ability to grow on and degrade phenols as sole carbon sources in aerobic shaking batch culture. The organism degraded up to 1000 ppm of phenol using meta cleavage pathway. The pathways for phenol degradation were proposed by the identification of metabolites and assay of ring cleavage enzymes in cell extracts. Phenol was degraded via catechol with subsequent metaring cleavage. Cell growth increased as the phenol concentrations increased up to 1000 ppm phenol. The biodegradation efficiency, degradation extent, and metabolic pathway of phenol were determined to provide useful clues for further application of this isolate in the engineered bioremediation systems. The paper's results suggest that Pseudomonas fluorescens PU1 strain could be a good candidate for remediation of phenol contaminants from heavily polluted sites.","PeriodicalId":14849,"journal":{"name":"ISRN Microbiology","volume":"2012 ","pages":"741820"},"PeriodicalIF":0.0,"publicationDate":"2012-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5402/2012/741820","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31471313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 89

Production of Quorum Sensing Inhibitors in Growing Onion Bulbs Infected with Pseudomonas aeruginosa E (HQ324110). 感染铜绿假单胞菌E (HQ324110)的洋葱鳞茎生长中群体感应抑制剂的产生

ISRN Microbiology Pub Date : 2012-01-05 Print Date: 2012-01-01 DOI: 10.5402/2012/161890

Mohamed H Abd-Alla, Shymaa R Bashandy

{"title":"Production of Quorum Sensing Inhibitors in Growing Onion Bulbs Infected with Pseudomonas aeruginosa E (HQ324110).","authors":"Mohamed H Abd-Alla, Shymaa R Bashandy","doi":"10.5402/2012/161890","DOIUrl":"https://doi.org/10.5402/2012/161890","url":null,"abstract":"Eighteen organic compounds were present in growing onion bulbs cultivar Giza 6 infected with P. aeruginosa, but only fourteen of them are present in dry infected onion bulbs; however, four compounds were missing in dry onion. The missing compounds in dry infected onion bulbs are pantolactone, 4,5-dihydro-4,5-dimethylfuran-2(3H)-one, myristic acid, and linoleic acid. All of them were detected in growing onion (living cell) during Pseudomonas aeruginosa infection, and it is hypothesized that it may be produced by plants and act as defence system. Pantolactone and myristic acid were selected to explore their effects on growth and virulence factors of Pseudomonas aeruginosa. Exogenous application of pantolactone and myristic acid significantly inhibited pyocyanin production, protease, and lipase and polygalacturonase activity but did not have any significant effects on bacterial growth. The inhibition of virulence factors without reduction in bacterial growth may be providing strong support that these chemical molecules are general quorum sensing inhibitors than an antibacterial effect. Disruption of quorum sensing of pathogen indicates that this new approach has potential in fighting bacterial infections in human and plants.","PeriodicalId":14849,"journal":{"name":"ISRN Microbiology","volume":"2012 ","pages":"161890"},"PeriodicalIF":0.0,"publicationDate":"2012-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5402/2012/161890","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31470249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 19

Diphtheria, tetanus, and pertussis immunity in Indian adults and immunogenicity of td vaccine. 印度成年人的白喉、破伤风和百日咳免疫力以及白喉、破伤风和百日咳疫苗的免疫原性。

ISRN Microbiology Pub Date : 2011-12-28 Print Date: 2011-01-01 DOI: 10.5402/2011/745868

Prasad S Kulkarni, Sidram K Raut, Sanjay P Dhorje, Prajakt J Barde, Girish Koli, Suresh S Jadhav

引用次数: 0

Impact of Fungicides Chlorothalonil and Propiconazole on Microbial Activities in Groundnut (Arachis hypogaea L.) Soils. 杀菌剂百菌清和丙环康唑对花生微生物活性的影响的土壤。

ISRN Microbiology Pub Date : 2011-12-25 Print Date: 2011-01-01 DOI: 10.5402/2011/623404

A C Ramudu, G Jaffer Mohiddin, M Srinivasulu, M Madakka, V Rangaswamy

{"title":"Impact of Fungicides Chlorothalonil and Propiconazole on Microbial Activities in Groundnut (Arachis hypogaea L.) Soils.","authors":"A C Ramudu, G Jaffer Mohiddin, M Srinivasulu, M Madakka, V Rangaswamy","doi":"10.5402/2011/623404","DOIUrl":"https://doi.org/10.5402/2011/623404","url":null,"abstract":"Introduction of agrochemicals (fungicides) into soil may have lasting effects on soil microbial activities and thus affect soil health. In order to determine the changes in microbial activity in a black clay and red sandy loam soils of groundnut (Arachis hypogaea L.) cultivated fields, a case study was conducted with propiconazole and chlorothalonil to evaluate its effects on soil enzymes (cellulase and invertase) throughout 40 days of incubation under laboratory conditions with different concentrations (1.0, 2.5, 5.0, 7.5, and 10.0 kg ha(-1)). Individual application of the two fungicides at 1.0, 2.5, and 5.0 kg ha(-1) to the soil distinctly enhanced the activities of cellulase and invertase but at higher concentrations of 7.5 and 10 kg ha(-1) was toxic or innocuous to both cellulase and invertase activities. In soil samples receiving 2.5-5.0 kg ha(-1) of the fungicides, the accumulation of reducing sugar was pronounced more at 20 days, and the activity of the cellulase and invertase was drastically decreased with increasing period of incubation up to 30 and 40 days.","PeriodicalId":14849,"journal":{"name":"ISRN Microbiology","volume":"2011 ","pages":"623404"},"PeriodicalIF":0.0,"publicationDate":"2011-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5402/2011/623404","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31563732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 24

Reduction of turbidity of water using locally available natural coagulants. 使用当地可用的天然混凝剂降低水的浑浊度。

ISRN Microbiology Pub Date : 2011-12-19 Print Date: 2011-01-01 DOI: 10.5402/2011/632189

Md Asrafuzzaman, A N M Fakhruddin, Md Alamgir Hossain

引用次数: 165

Reduction of Adherence of E. coli O157:H7 to HEp-2 Cells and to Bovine Large Intestinal Mucosal Explants by Colicinogenic E. coli. 大肠杆菌O157:H7对HEp-2细胞和牛大肠黏膜外植体粘附性的影响

ISRN Microbiology Pub Date : 2011-12-11 Print Date: 2011-01-01 DOI: 10.5402/2011/697020

A I Etcheverría, G H Arroyo, R Alzola, A E Parma

引用次数: 4

Detection of Trichomonas vaginalis in Vaginal Swab Clinical Samples from Palestinian Women by Culture. 巴勒斯坦妇女阴道拭子临床标本中阴道毛滴虫培养检测。

ISRN Microbiology Pub Date : 2011-12-10 Print Date: 2011-01-01 DOI: 10.5402/2011/872358

Yasmeen Houso, Mohammad A Farraj, As'ad Ramlawi, Tamer Essawi

引用次数: 7

Prevalence of ESBL-Producing Klebsiella pneumoniae Isolates in Tertiary Care Hospital. 三级医院产esbl肺炎克雷伯菌分离株的流行情况。

ISRN Microbiology Pub Date : 2011-12-01 Print Date: 2011-01-01 DOI: 10.5402/2011/318348

Vemula Sarojamma, Vadde Ramakrishna

{"title":"Prevalence of ESBL-Producing Klebsiella pneumoniae Isolates in Tertiary Care Hospital.","authors":"Vemula Sarojamma, Vadde Ramakrishna","doi":"10.5402/2011/318348","DOIUrl":"10.5402/2011/318348","url":null,"abstract":"Extended-spectrum β lactamases (ESBLs) continue to be a major challenge in clinical setups world over, conferring resistance to the expanded-spectrum cephalosporins. An attempt was made to study the prevalence of ESBL-producing Klebsiella pneumoniae clinical isolates in a tertiary care hospital in Kurnool. A total of hundred collected isolates of Klebsiella pneumoniae was studied for their susceptibility patterns to various antibiotics and detection of ESBL producers by double disc synergy test (DDST) and phenotypic confirmatory disc diffusion test (PCDDT). Of the 100 isolates tested for their antibiogram, 61% isolates have shown susceptibility to 3rd-generation cepholosporins and 39% were resistant. Amoxycillin showed the highest percentage of resistance followed by tetracyclins and cotrimoxazole. Among 39 resistant isolates of Klebsiella pneumoniae, 17 were ESBL producers detected by DDST and PCDDT. ESBL producers were more in the hospital isolates (28%) compared to community isolates (6%). Maximum percentage of ESBL producers were noticed from blood sample with 57.14%. In the present study, a large number of isolates were found to be multidrug resistant and ESBL producers. PCDDT was found to be better than DDST in the detection of ESBLs. Continued monitoring of drug resistance is necessary in clinical settings for proper disease management.","PeriodicalId":14849,"journal":{"name":"ISRN Microbiology","volume":"2011 ","pages":"318348"},"PeriodicalIF":0.0,"publicationDate":"2011-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5402/2011/318348","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31563729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 70

Genotyping of the pseudorabies virus by multiplex PCR followed by restriction enzyme analysis. 伪狂犬病毒多重PCR基因分型及限制性内切酶分析。

ISRN Microbiology Pub Date : 2011-11-24 Print Date: 2011-01-01 DOI: 10.5402/2011/458294

Antônio Augusto Fonseca, Cristina Gonçalves Magalhães, Erica Bravo Sales, Régia Maria D'Ambros, Janice Ciacci-Zanella, Marcos Bryan Heinemann, Rômulo Cerqueira Leite, Jenner Karlisson Pimenta Dos Reis

引用次数: 4