{"title":"利用金合欢种子进行分批饲喂发酵,提高枕青霉的甘露聚糖酶产量","authors":"Monia Blibech, Raoudha Ellouz Ghorbel, Fatma Chaari, Ilyes Dammak, Fatma Bhiri, Mohamed Neifar, Semia Ellouz Chaabouni","doi":"10.5402/2011/938347","DOIUrl":null,"url":null,"abstract":"<p><p>By applying a fed-batch strategy, production of Penicillium occitanis mannanases could be almost doubled as compared to a batch cultivation on acacia seeds (76 versus 41 U/mL). Also, a 10-fold increase of enzyme activities was observed from shake flask fermentation to the fed-batch fermentation. These production levels were 3-fold higher than those obtained on coconut meal. The high mannanase production using acacia seeds powder as inducer substrate showed the suitability of this culture process for industrial-scale development.</p>","PeriodicalId":14849,"journal":{"name":"ISRN Microbiology","volume":"2011 ","pages":"938347"},"PeriodicalIF":0.0000,"publicationDate":"2011-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3658641/pdf/","citationCount":"0","resultStr":"{\"title\":\"Improved Mannanase Production from Penicillium occitanis by Fed-Batch Fermentation Using Acacia Seeds.\",\"authors\":\"Monia Blibech, Raoudha Ellouz Ghorbel, Fatma Chaari, Ilyes Dammak, Fatma Bhiri, Mohamed Neifar, Semia Ellouz Chaabouni\",\"doi\":\"10.5402/2011/938347\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>By applying a fed-batch strategy, production of Penicillium occitanis mannanases could be almost doubled as compared to a batch cultivation on acacia seeds (76 versus 41 U/mL). Also, a 10-fold increase of enzyme activities was observed from shake flask fermentation to the fed-batch fermentation. These production levels were 3-fold higher than those obtained on coconut meal. The high mannanase production using acacia seeds powder as inducer substrate showed the suitability of this culture process for industrial-scale development.</p>\",\"PeriodicalId\":14849,\"journal\":{\"name\":\"ISRN Microbiology\",\"volume\":\"2011 \",\"pages\":\"938347\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2011-10-24\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3658641/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"ISRN Microbiology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.5402/2011/938347\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2011/1/1 0:00:00\",\"PubModel\":\"Print\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"ISRN Microbiology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5402/2011/938347","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2011/1/1 0:00:00","PubModel":"Print","JCR":"","JCRName":"","Score":null,"Total":0}
Improved Mannanase Production from Penicillium occitanis by Fed-Batch Fermentation Using Acacia Seeds.
By applying a fed-batch strategy, production of Penicillium occitanis mannanases could be almost doubled as compared to a batch cultivation on acacia seeds (76 versus 41 U/mL). Also, a 10-fold increase of enzyme activities was observed from shake flask fermentation to the fed-batch fermentation. These production levels were 3-fold higher than those obtained on coconut meal. The high mannanase production using acacia seeds powder as inducer substrate showed the suitability of this culture process for industrial-scale development.