{"title":"Improved Mannanase Production from Penicillium occitanis by Fed-Batch Fermentation Using Acacia Seeds.","authors":"Monia Blibech, Raoudha Ellouz Ghorbel, Fatma Chaari, Ilyes Dammak, Fatma Bhiri, Mohamed Neifar, Semia Ellouz Chaabouni","doi":"10.5402/2011/938347","DOIUrl":null,"url":null,"abstract":"<p><p>By applying a fed-batch strategy, production of Penicillium occitanis mannanases could be almost doubled as compared to a batch cultivation on acacia seeds (76 versus 41 U/mL). Also, a 10-fold increase of enzyme activities was observed from shake flask fermentation to the fed-batch fermentation. These production levels were 3-fold higher than those obtained on coconut meal. The high mannanase production using acacia seeds powder as inducer substrate showed the suitability of this culture process for industrial-scale development.</p>","PeriodicalId":14849,"journal":{"name":"ISRN Microbiology","volume":"2011 ","pages":"938347"},"PeriodicalIF":0.0000,"publicationDate":"2011-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3658641/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"ISRN Microbiology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5402/2011/938347","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2011/1/1 0:00:00","PubModel":"Print","JCR":"","JCRName":"","Score":null,"Total":0}
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Abstract
By applying a fed-batch strategy, production of Penicillium occitanis mannanases could be almost doubled as compared to a batch cultivation on acacia seeds (76 versus 41 U/mL). Also, a 10-fold increase of enzyme activities was observed from shake flask fermentation to the fed-batch fermentation. These production levels were 3-fold higher than those obtained on coconut meal. The high mannanase production using acacia seeds powder as inducer substrate showed the suitability of this culture process for industrial-scale development.
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