Genetic Diversity and Drug Resistance Mutations in HIV-1 from Untreated Patients in Niamey, Niger.

ISRN Microbiology Pub Date : 2011-11-03 Print Date: 2011-01-01 DOI:10.5402/2011/797463

Saïdou Mamadou, Yahayé Hanki, Amadou Roufaï Ali Maazou, Balki Aoula, Sanata Diallo

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引用次数: 7

Abstract

The objective of the study was to estimate the prevalence of transmitted resistance to antiretroviral of HIV-1 circulating in Niger. We collected plasmas from 96 drug-naive patients followed up in the main HIV/AIDS Care Center of Niamey, the capital city of Niger. After RNA extraction and retrotranscription to proviral DNA, nested PCR was performed to amplify PR (codons 1-99) and RT (codons 1-240) fragments for sequencing. Sequences were analysed for phylogeny, then for resistance-associated mutations according to IAS-USA and Stanford's lists of mutations. We characterized six HIV-1 genetic variants: CRF02-AG (56.3%), CRF30_0206 (15.6%), subtype G (15.6%), CRF06_cpx (9.4%), CRF11_cpx (2.1%), and CRF01_AE (1%). About 8.3% of HIV strains had at least 1 resistance mutation: 4 strains with at least 1 mutation to NRTI, 5 for NNRTI, and 1 for PI, respectiveley 4.2%, 5.2%, and 1.0%. These preliminary results gave enough information for the need of instauring HIV drug resistance national surveillance.

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尼日尔尼亚美未经治疗的HIV-1患者的遗传多样性和耐药突变

该研究的目的是估计尼日尔流行的HIV-1传播抗逆转录病毒耐药性的流行情况。我们收集了在尼日尔首都尼亚美主要艾滋病毒/艾滋病护理中心随访的96名药物初治患者的血浆。提取RNA逆转录至病毒前DNA后，进行巢式PCR扩增PR(密码子1-99)和RT(密码子1-240)片段进行测序。对序列进行系统发育分析，然后根据IAS-USA和Stanford突变列表分析抗性相关突变。我们鉴定了6种HIV-1基因变异:CRF02-AG(56.3%)、CRF30_0206(15.6%)、G亚型(15.6%)、CRF06_cpx(9.4%)、CRF11_cpx(2.1%)和CRF01_AE(1%)。约8.3%的HIV毒株存在至少1个耐药突变，其中4株对NRTI、5株对NNRTI、1株对PI存在至少1个耐药突变，分别为4.2%、5.2%和1.0%。这些初步结果为加强国家艾滋病毒耐药性监测的必要性提供了足够的信息。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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ISRN Microbiology

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