Japanese journal of infectious diseases最新文献

Evaluation of rapid amplicon-based nanopore sequencing using the latest chemistry for accurate whole genome analysis of influenza A virus in clinical samples.

IF 1.3 4区医学

Japanese journal of infectious diseases Pub Date : 2025-01-31 DOI: 10.7883/yoken.JJID.2024.400

Yumani Kuba, Nobuhiro Takemae, Satoshi Kawato, Kunihiro Oba, Kiyosu Taniguchi, Tsutomu Kageyama

{"title":"Evaluation of rapid amplicon-based nanopore sequencing using the latest chemistry for accurate whole genome analysis of influenza A virus in clinical samples.","authors":"Yumani Kuba, Nobuhiro Takemae, Satoshi Kawato, Kunihiro Oba, Kiyosu Taniguchi, Tsutomu Kageyama","doi":"10.7883/yoken.JJID.2024.400","DOIUrl":"https://doi.org/10.7883/yoken.JJID.2024.400","url":null,"abstract":"MinION sequencing is widely used to sequence influenza A virus (IAV) genomes; however, the accuracy and utility of this approach, using the latest chemistry to obtain whole viral genome sequences directly from clinical samples, remain insufficiently investigated. We evaluated the sequencing accuracy of combining simultaneous multisegment one-step RT-PCR and MinION sequencing using various subtypes of 13 IAV isolates. The latest R10.4.1 chemistry significantly improved sequencing accuracy, achieving ≥99.993% identity with Illumina MiSeq results and reducing the single nucleotide deletion in homopolymer regions. Applying this method to 11 clinical samples enabled rapid subtype identification and the acquisition of eight full-length IAV genomes. In four of these samples, subtype identification of HA and NA was achieved within 20 min after the start of sequencing and a full-length IAV genome was obtained within 7 h after RNA extraction. However, there was concern that cross barcode misassignment during demultiplexing affected data interpretation, particularly for samples with low viral genome copy numbers. This approach can be used for the rapid identification of IAV subtypes and accurate acquisition of full IAV genome sequences from clinical samples, although careful data analysis is required for the multiplex sequencing of clinical samples with low viral genome copy numbers.","PeriodicalId":14608,"journal":{"name":"Japanese journal of infectious diseases","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143079439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Seroprevalence of hepatitis A virus and hepatitis E virus antibodies in Madhesh Province, Dhanusha, Nepal.

IF 1.3 4区医学

Japanese journal of infectious diseases Pub Date : 2025-01-31 DOI: 10.7883/yoken.JJID.2024.321

Sandesh Rimal, Sabin Shrestha, Aashma Khadka, Mami Matsuda, Ryosuke Suzuki, Shyam Prakash Dumre, Basu Dev Pandey

引用次数: 0

Detection of recombinant type of human adenovirus C composed of type 89 and type 5 in Aichi Prefecture, Japan, 2016-2019.

IF 1.3 4区医学

Japanese journal of infectious diseases Pub Date : 2025-01-31 DOI: 10.7883/yoken.JJID.2024.246

Emi Hirose, Hiroko Minagawa, Hirokazu Adachi, Masakazu Suzuki, Noriko Nakamura, Noriko Saito, Miyabi Ito, Chikako Mitsuoka, Katsuhiko Sato, Yoshihiro Yasui

{"title":"Detection of recombinant type of human adenovirus C composed of type 89 and type 5 in Aichi Prefecture, Japan, 2016-2019.","authors":"Emi Hirose, Hiroko Minagawa, Hirokazu Adachi, Masakazu Suzuki, Noriko Nakamura, Noriko Saito, Miyabi Ito, Chikako Mitsuoka, Katsuhiko Sato, Yoshihiro Yasui","doi":"10.7883/yoken.JJID.2024.246","DOIUrl":"https://doi.org/10.7883/yoken.JJID.2024.246","url":null,"abstract":"Recombinant human adenoviruses (HAdVs) have been reported from many countries, including Japan. We report a recombinant HAdV-C type detected in feces with gastroenteritis infection or throat swabs with upper respiratory tract inflammation of six children in Aichi Prefecture. This type may have been prevalent in the area. Between April 2014 and March 2019, we attempted to detect HAdVs in 9,483 specimens (fecal, throat swab, urine, and conjunctival swab) collected from patients with suspected viral infection. A total of 220 HAdV-C isolates were obtained and serotyped by neutralization test (NT). Of these, 62 HAdV-C strains (HAdV-C1: 15, -C2: 19, -C5: 22, -C6: 6) were genotyped by sequencing for the three regions, penton base, hexon, and fiber regions, respectively. All 62 strains were grouped into the same genotype as serotype for the hexon and fiber region. In contrast, phylogenetic analysis showed 6 of the 22 strains with serotype HAdV-C5 were grouped into HAdV-C89 for the penton base region. This type was detected as a recombinant HAdV causing SARI in China between 2017 and 2021, was found to have been persistently detected in transnational Aichi Prefecture over the same period.","PeriodicalId":14608,"journal":{"name":"Japanese journal of infectious diseases","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143079538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Tokyo Metropolitan Government external quality assessment of SARS-CoV-2 nucleic acid amplification tests for clinics in 2023.

IF 1.3 4区医学

Japanese journal of infectious diseases Pub Date : 2025-01-31 DOI: 10.7883/yoken.JJID.2024.205

Rie Moriuchi, Ryouta Inaba, Miyuki Nagano, Mayuko Oda, Takayuki Shinkai, Toshihisa Noguchi, Yoshiyuki Sugishita, Itaru Nishizuka, Yoshikazu Ishii

{"title":"The Tokyo Metropolitan Government external quality assessment of SARS-CoV-2 nucleic acid amplification tests for clinics in 2023.","authors":"Rie Moriuchi, Ryouta Inaba, Miyuki Nagano, Mayuko Oda, Takayuki Shinkai, Toshihisa Noguchi, Yoshiyuki Sugishita, Itaru Nishizuka, Yoshikazu Ishii","doi":"10.7883/yoken.JJID.2024.205","DOIUrl":"https://doi.org/10.7883/yoken.JJID.2024.205","url":null,"abstract":"An external quality assessment (EQA) on nucleic acid amplification tests for SARS-CoV-2 was conducted among clinics that introduced testing instrument through the Tokyo Subsidy Program for Facilities and Equipment for Testing in COVID-19 in 2023. The survey samples consisted of sample 1 (20,000 copies/mL), sample 2 (10,000 copies/mL), and negative sample. Results were obtained through Cycle threshold (Ct) values as well as positive-negative result determinations. On-site technical support was provided to the clinics that responded incorrectly. A total of 544 clinics (589 instruments) participated, and Abbott's ID NOW TM (82.2%) was the most widely used in this survey. The qualitative test results showed high correctness rates, 95.2% for sample 1, 93.2% for sample 2, and 96.3% for negative sample. Differences in Ct values were observed among reagents and instruments. Technical support were provided to 63 clinics. It revealed that discrepancies of qualitative test were mainly due to deviations from the protocol instructions, mixing all three survey samples into one vial and errors caused by foaming. This survey was unique in that the participants were mostly end-users who were not laboratory testing specialists and mainly used point-of-care testing instruments. It is important to continue EQA to improve and maintain testing accuracy.","PeriodicalId":14608,"journal":{"name":"Japanese journal of infectious diseases","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143079801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Monkeypox outbreak 2023 in Chengdu, China: an observational study.

IF 1.3 4区医学

Japanese journal of infectious diseases Pub Date : 2025-01-31 DOI: 10.7883/yoken.JJID.2024.168

Lang Rao, Mei Yang, Li-Yu Chen, Li-Huang Chen, Yong-Hong Lu, Ying Wang, Yan-Yan Feng

{"title":"Monkeypox outbreak 2023 in Chengdu, China: an observational study.","authors":"Lang Rao, Mei Yang, Li-Yu Chen, Li-Huang Chen, Yong-Hong Lu, Ying Wang, Yan-Yan Feng","doi":"10.7883/yoken.JJID.2024.168","DOIUrl":"https://doi.org/10.7883/yoken.JJID.2024.168","url":null,"abstract":"Monkeypox is a zoonotic viral disease caused by monkeypox virus infection and formerly this disease had been endemic mainly in Africa. Since May 2022, the disease has rapidly spread across the world and some imported case was confirmed sporadically in China. Under these circumstances, data of confirmed cases were collected consecutively in Chengdu, southwest China to investigate clinical and epidemiological characteristics. In this study, 28 male patients were confirmed to have monkeypox infection, with 26 identifying as MSM (men who have sex with men). Typical lesions included papules, pustular papules, vesicles, scabs and ulcerations. The most common sites of skin lesions were anogenital and perineal areas, three patients developed a widespread exanthem. Seven patients reported a history of sexual transmitted diseases, no one was hospitalized and there were no deaths. Three instances of secondary transmission were identified among close contacts, all of them were sexual partners of our participants. Furthermore, this observation provides insights of the public health for surveillance of this emerging disease and raises awareness among people at risk.","PeriodicalId":14608,"journal":{"name":"Japanese journal of infectious diseases","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143079440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation of an Outbreak of Clostridium perfringens in Toyama, Japan, 2023 Using Single-Nucleotide Polymorphism Analysis for Genotyping. 利用单核苷酸多态性分析进行基因分型的日本富山梭状芽孢杆菌疫情调查，2023 年。

IF 1.3 4区医学

Japanese journal of infectious diseases Pub Date : 2025-01-23 Epub Date: 2024-09-30 DOI: 10.7883/yoken.JJID.2024.189

Kazuki Saito, Keiko Kimata, Masanori Watahiki, Junko Isobe, Jun-Ichi Kanatani, Kaho Ikeda, Takashi Takeuchi, Chiharu Matsuzaki, Kazunori Oishi

{"title":"Investigation of an Outbreak of Clostridium perfringens in Toyama, Japan, 2023 Using Single-Nucleotide Polymorphism Analysis for Genotyping.","authors":"Kazuki Saito, Keiko Kimata, Masanori Watahiki, Junko Isobe, Jun-Ichi Kanatani, Kaho Ikeda, Takashi Takeuchi, Chiharu Matsuzaki, Kazunori Oishi","doi":"10.7883/yoken.JJID.2024.189","DOIUrl":"10.7883/yoken.JJID.2024.189","url":null,"abstract":"Clostridium perfringens, which produces C. perfringens enterotoxin (CPE), is a major cause of food poisoning because of its gastrointestinal toxicity. In outbreaks of C. perfringens, genotyping is important for identifying the source. We genotyped strains isolated from an outbreak of food poisoning in Toyama Prefecture in 2023, using single-nucleotide polymorphism (SNP) analysis. The strains of C. perfringens were isolated from samples of curry consumed by all the affected patients and from the feces of patients and staff worker. The enterotoxin gene (cpe) was detected in isolates from patients and curry samples. The cpe-negative isolates were found in patients who had consumed curry and in the staff worker. The results of the SNP analysis suggested that the patient and curry isolates were likely from the same source but were unlikely to be related to the staff isolates. The results of SNP and pulsed-field gel electrophoresis (PFGE) analyses were consistent, indicating that the patient and curry isolates originated from the same source. SNP analysis, a whole-genome-based genotyping method, is a promising alternative to traditional PFGE for investigating outbreaks. Further studies are needed to accumulate more experience with genotyping using SNP analysis for the epidemiological investigation of outbreaks of C. perfringens.","PeriodicalId":14608,"journal":{"name":"Japanese journal of infectious diseases","volume":" ","pages":"47-50"},"PeriodicalIF":1.3,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142346832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SARS-CoV-2 IgG Seroprevalence in the Okinawa Main Island and Remote Islands in Okinawa, Japan, 2020-2021. 2020-2021 年日本冲绳本岛和冲绳偏远岛屿的 SARS-CoV-2 IgG 血清流行率。

IF 1.3 4区医学

Japanese journal of infectious diseases Pub Date : 2025-01-23 Epub Date: 2024-09-30 DOI: 10.7883/yoken.JJID.2023.255

Yoshihiro Takayama, Yusuke Shimakawa, Yoshiaki Aizawa, Christian Butcher, Naomi Chibana, Mary Collins, Kohei Kamegai, Tae Gyun Kim, Satoshi Koyama, Ryota Matsuyama, Melissa M Matthews, Tomoari Mori, Tetsuharu Nagamoto, Masashi Narita, Ryosuke Omori, Noriko Shibata, Satoshi Shibata, Souichi Shiiki, Shunichi Takakura, Naoki Toyozato, Hiroyuki Tsuchiya, Matthias Wolf, Taro Yamamoto, Shuhei Yokoyama, Sho Yonaha, Kenji Mizumoto

{"title":"SARS-CoV-2 IgG Seroprevalence in the Okinawa Main Island and Remote Islands in Okinawa, Japan, 2020-2021.","authors":"Yoshihiro Takayama, Yusuke Shimakawa, Yoshiaki Aizawa, Christian Butcher, Naomi Chibana, Mary Collins, Kohei Kamegai, Tae Gyun Kim, Satoshi Koyama, Ryota Matsuyama, Melissa M Matthews, Tomoari Mori, Tetsuharu Nagamoto, Masashi Narita, Ryosuke Omori, Noriko Shibata, Satoshi Shibata, Souichi Shiiki, Shunichi Takakura, Naoki Toyozato, Hiroyuki Tsuchiya, Matthias Wolf, Taro Yamamoto, Shuhei Yokoyama, Sho Yonaha, Kenji Mizumoto","doi":"10.7883/yoken.JJID.2023.255","DOIUrl":"10.7883/yoken.JJID.2023.255","url":null,"abstract":"We estimated the seroprevalence of anti-severe acute respiratory syndrome coronavirus 2 (anti-SARS-CoV-2) immunoglobulin G (IgG) in different island groups in Okinawa. A cross-sectional serosurvey was conducted over three periods between July 2020 and February 2021. A total of 2,683 serum samples were collected from six referral medical centers, each covering a separate region in Okinawa. In the main island, the seroprevalence was 0.0% (0/392; 95% confidence interval (CI), 0.0- 0.9%), 0.6% (8/1,448, 0.2-1.1%), and 1.4% (8/582, 0.6-2.7%) during the 1st, 2nd, and 3rd serosurvey, respectively. In remote islands, the seroprevalence was 0.0% (0/144; 95% CI, 0.0-2.5%) and 1.6% (2/123, 0.2-5.8%) during the 2nd and 3rd survey, respectively. The case detection ratio was 2.7 (95% CI, 1.3-5.3) in the main island and 2.8 (0.7-11.1) in remote islands during the 3rd survey. The case detection ratio was the highest in individuals aged 20-29 years (8.3; 95% CI, 3.3-21.4) in the main island and in those aged 50-59 years (14.1; 2.1-92.7) in remote islands, suggesting underreporting of clinical cases by the surveillance system in these subgroups. A serosurvey conducted during an emerging infectious disease epidemic can be useful for validating the reliability of the surveillance system by providing case detection ratio.","PeriodicalId":14608,"journal":{"name":"Japanese journal of infectious diseases","volume":" ","pages":"8-18"},"PeriodicalIF":1.3,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142346836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Risk of Death in Older Japanese Adults with COVID-19 Caused by the Omicron Variant: a Population-Based Study. 日本 COVID-19 老年病例感染 Omicron 变体的死亡风险：一项基于人群的研究。

IF 1.3 4区医学

Japanese journal of infectious diseases Pub Date : 2025-01-23 Epub Date: 2024-09-30 DOI: 10.7883/yoken.JJID.2024.071

Hideo Tanaka, Yuki Takahashi, Yoshitaka Koga, Shunichi Takiguchi, Shigeru Ogimoto, Shizuyo Inaba, Hiroyuki Matsuoka, Yuka Miyajima, Takeshi Takagi, Fujiko Irie, Yoshihito Bamba, Fuyo Yoshimi, Tomoyuki Suzuki, Isao Araki, Chika Shirai, Sayuri Matsumoto, Toshiyuki Shibata, Hitomi Nagai, Masaru Kinoshita, Rie Fujita, Tsuyoshi Ogata

{"title":"Risk of Death in Older Japanese Adults with COVID-19 Caused by the Omicron Variant: a Population-Based Study.","authors":"Hideo Tanaka, Yuki Takahashi, Yoshitaka Koga, Shunichi Takiguchi, Shigeru Ogimoto, Shizuyo Inaba, Hiroyuki Matsuoka, Yuka Miyajima, Takeshi Takagi, Fujiko Irie, Yoshihito Bamba, Fuyo Yoshimi, Tomoyuki Suzuki, Isao Araki, Chika Shirai, Sayuri Matsumoto, Toshiyuki Shibata, Hitomi Nagai, Masaru Kinoshita, Rie Fujita, Tsuyoshi Ogata","doi":"10.7883/yoken.JJID.2024.071","DOIUrl":"10.7883/yoken.JJID.2024.071","url":null,"abstract":"We assessed case fatality rates (CFRs) in adults aged ≥70 years in 10 prefectures in Japan (14.8 million residents) diagnosed between January 2022 and March 2023, when the Omicron variant was dominant in Japan. We selected incident reports on 283,052 cases from participating public health centers reported according to the Infectious Diseases Control Law. Patients were passively followed up until the end of their isolation, date of death, or 28 days after COVID-19 diagnosis, whichever occurred first. We calculated age-standardized CFRs with 95% confidence intervals (CIs) using the Japanese population aged 70-79, 80-89 and ≥90 years in 2022, divided into 16 subgroups according to the period of COVID-19 diagnosis. The overall CFR was 1.59% (95% CI: 1.55-1.64%); ranging between 0.67% (95% CI: 0.38-0.96%, from May 23 to June 19, 2022) and 2.58% (95% CI: 2.36-2.80%, from January 31 to February 27, 2022). The age-standardized CFRs had three peaks, (2.2% from January 31 to February 27, 2022; 1.0% from July 18 to August 14, 2022; and 1.6% from December 26, 2022 to January 22, 2023) coinciding with the 6th, 7th, and 8th COVID-19 waves in Japan caused by the Omicron variant. Population-based CFRs for Omicron variant COVID-19 in adults aged ≥70 years remained <3% throughout the period January 2022 to March 2023, including during three large waves in Japan.","PeriodicalId":14608,"journal":{"name":"Japanese journal of infectious diseases","volume":" ","pages":"1-5"},"PeriodicalIF":1.3,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142346835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic Diversity and Potential Transmission of Escherichia albertii in a Poultry-Breeding Rural Village. 一个家禽养殖农村的白细胞埃希氏菌遗传多样性和潜在传播途径。

IF 1.3 4区医学

Japanese journal of infectious diseases Pub Date : 2025-01-23 Epub Date: 2024-09-30 DOI: 10.7883/yoken.JJID.2024.209

Peihua Zhang, Guodong Yan, Qian Liu, Xi Yang, Jie Zhang, Xi Chen, Hong Wang, Ling Zhang, Xinxia Sui, Xiangning Bai, Yanwen Xiong, Zhengdong Zhang

{"title":"Genetic Diversity and Potential Transmission of Escherichia albertii in a Poultry-Breeding Rural Village.","authors":"Peihua Zhang, Guodong Yan, Qian Liu, Xi Yang, Jie Zhang, Xi Chen, Hong Wang, Ling Zhang, Xinxia Sui, Xiangning Bai, Yanwen Xiong, Zhengdong Zhang","doi":"10.7883/yoken.JJID.2024.209","DOIUrl":"10.7883/yoken.JJID.2024.209","url":null,"abstract":"Escherichia albertii is an emerging foodborne pathogen that causes diarrhea. Although various animals, especially poultry, serve as reservoirs of E. albertii, the transmission of E. albertii among reservoirs and the associated risks to humans remain unclear. This study investigated an E. albertii-infected infant exposed to poultry, and collected samples from contact persons, poultry, and the environment to better understand the transmission dynamics of E. albertii. One E. albertii isolate from a contact person, seven isolates from poultry, and six isolates from the environment were recovered. Whole-genome sequencing analysis showed that eight strains derived from poultry or environment and classified as ST4633 shared great similarity (core genome single-nucleotide polymorphisms [cgSNPs] ≤20). However, the patient-derived strain ESA311 had a cgSNP difference of 1165 with the human strain ESA339 and differed from poultry and environmental strains (cgSNP range 2417-14997), indicating distant relatedness. The whole-genome phylogeny showed that several human-derived E. albertii strains clustered with those of animal origin. Our results suggest that family-bred poultry constitute a possible reservoir for E. albertii, with the environment acting as a crucial vector for the spread of these bacteria and posing a risk to humans. Further surveillance of poultry is required to elucidate the public health risks associated with E. albertii infections.","PeriodicalId":14608,"journal":{"name":"Japanese journal of infectious diseases","volume":" ","pages":"19-27"},"PeriodicalIF":1.3,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142346831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chemotactic Activity of Products of Elizabethkingia anophelis Derived from Aedes albopictus against RAW264 Murine Macrophage Cell Line. 从白纹伊蚊中提取的 Elizabethkingia anophelis 产品对 RAW264 鼠巨噬细胞系的趋化活性。

IF 1.3 4区医学

Japanese journal of infectious diseases Pub Date : 2025-01-23 Epub Date: 2024-10-31 DOI: 10.7883/yoken.JJID.2024.227

Satoru Yui, Norihiko Fujii, Jo Terauchi, Nana Tanabe, Marie Kanno, Kouta Umehara, Ryosuke Iijima, Riyo Kamata, Naoki Ohkura, Seishi Kishimoto, Toshinori Sasaki

{"title":"Chemotactic Activity of Products of Elizabethkingia anophelis Derived from Aedes albopictus against RAW264 Murine Macrophage Cell Line.","authors":"Satoru Yui, Norihiko Fujii, Jo Terauchi, Nana Tanabe, Marie Kanno, Kouta Umehara, Ryosuke Iijima, Riyo Kamata, Naoki Ohkura, Seishi Kishimoto, Toshinori Sasaki","doi":"10.7883/yoken.JJID.2024.227","DOIUrl":"10.7883/yoken.JJID.2024.227","url":null,"abstract":"Dengue viruses enter the dermal macrophages derived from other tissues following a bite from an infected mosquito. We examined the chemotactic activity of factors derived from the dengue vector mosquito Aedes albopictus on a RAW264 murine macrophage cell line. We found that Elizabethkingia anophelis isolated from the mosquitoes exhibits migration-inducing activity in RAW264 cells. The active substances that induce the chemotactic movement were extracted using ethyl acetate. Chemotactic activity was noted in several of the fractions isolated using reverse-phase chromatography, suggesting that multiple components were responsible for this activity. Next, we isolated three bacterial colonies from wild A. albopictus mosquitoes collected from Toyama Park (Tokyo, Japan). The bacterial 16S rRNA gene sequences shared homology with that of Lonsdalea quercina. These bacteria also exhibited migration-inducing activity in RAW264 cells. The migration-inducing activity of the bacteria in mosquitoes may be a novel aspect of mosquito-mediated viral infections.","PeriodicalId":14608,"journal":{"name":"Japanese journal of infectious diseases","volume":" ","pages":"35-42"},"PeriodicalIF":1.3,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142545482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0