Japanese journal of infectious diseases最新文献

A case of vertebral osteomyelitis with an erector spinae abscess caused by Staphylococcus argenteus ST1223. 由阿根廷葡萄球菌ST1223引起的椎体骨髓炎伴竖脊脓肿1例。

IF 1.1 4区医学

Japanese journal of infectious diseases Pub Date : 2025-09-30 DOI: 10.7883/yoken.JJID.2025.116

Yusuke Watanabe, Naoto Takenoshita, Maki Tsuchiya, Yuri Miura, Kohji Komori, Tetsuo Yamaguchi, Itaru Nakamura, Hidehiro Watanabe, Soichiro Shimizu

{"title":"A case of vertebral osteomyelitis with an erector spinae abscess caused by Staphylococcus argenteus ST1223.","authors":"Yusuke Watanabe, Naoto Takenoshita, Maki Tsuchiya, Yuri Miura, Kohji Komori, Tetsuo Yamaguchi, Itaru Nakamura, Hidehiro Watanabe, Soichiro Shimizu","doi":"10.7883/yoken.JJID.2025.116","DOIUrl":"https://doi.org/10.7883/yoken.JJID.2025.116","url":null,"abstract":"Staphylococcus argenteus is a novel bacterial species distinct from Staphylococcus aureus. Nevertheless, S. argenteus is often misidentified as S. aureus because they cannot be differentiated easily using routine diagnostic microbiology methods. An 81-year-old man was admitted because of persistent lumbar pain and fever. Strains from his blood cultures were initially identified as methicillin-susceptible S. aureus. Enhanced computed tomography revealed an erector spinae abscess connected with the L5 transverse process, and magnetic resonance imaging of the lumbar region showed a high intensity of L1 and L5 on short tau inversion recovery. The abovementioned pathogen was also cultured from the aspiration fluid of the abscess. Therefore, the patient was diagnosed with vertebral osteomyelitis with an erector spinae abscess. We analyzed the detected strain using matrix-assisted laser desorption ionization time-of-flight mass spectrometry and whole-genome sequencing, re-identifying the strain as S. argenteus sequence type (ST)1223. Although S. argenteus ST1223 has mainly enteric pathogenicity, our case suggests its potential to invade the musculoskeletal system. Its pathogenicity was similar to or greater than that of S. aureus in both previous studies and our case. Thus, the clinical differentiation of S. argenteus and S. aureus warrants further investigation.","PeriodicalId":14608,"journal":{"name":"Japanese journal of infectious diseases","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145206692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of Viral Load in The Nasopharyngeal Swabs of Patients with Severe Acute Respiratory Syndrome Coronavirus 2 in Different Epidemic Seasons in Gunma Prefecture, Japan. 日本群马县不同流行季节SARS-CoV-2患者鼻咽拭子病毒载量比较

IF 1.1 4区医学

Japanese journal of infectious diseases Pub Date : 2025-09-19 Epub Date: 2024-12-27 DOI: 10.7883/yoken.JJID.2024.301

Yuki Nakazawa, Hiroyuki Tsukagoshi, Ryo Shimada, Rina Kubota, Nobuhiro Saruki

{"title":"Comparison of Viral Load in The Nasopharyngeal Swabs of Patients with Severe Acute Respiratory Syndrome Coronavirus 2 in Different Epidemic Seasons in Gunma Prefecture, Japan.","authors":"Yuki Nakazawa, Hiroyuki Tsukagoshi, Ryo Shimada, Rina Kubota, Nobuhiro Saruki","doi":"10.7883/yoken.JJID.2024.301","DOIUrl":"10.7883/yoken.JJID.2024.301","url":null,"abstract":"The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has repeatedly undergone mutations since its emergence, based on which, it has been assumed that there have been changes in its characteristics, including virulence and antigenicity. In this study, the viral loads in nasopharyngeal samples of patients with SARS-CoV-2 in Gunma Prefecture, Japan, from April 2, 2020, to April 1, 2023, were determined. The number of virus in samples in the Omicron-variant-prevalent period was higher than that in strains detected in samples before Week 50 of 2020; the B.1.1.284-prevalent period, the Alpha-variant-prevalent period, and the Delta-variant-prevalent period. Moreover, among the Omicron variants, the sub-lineage BA.5-prevalent period had more viral copies in the samples than in the BA.1-prevalent and BA.2-prevalent periods. Hence, the new variant may have released more viruses into the nasopharynx during the process of repeated mutations, resulting in widespread infection. The number of viruses detected in the nasopharyngeal samples showed an increasing trend with the evolution of the virus. Therefore, considering that the number of viruses in samples is also a vital factor contributing to the spread of the infection, it is important to determine the viral load in samples.","PeriodicalId":14608,"journal":{"name":"Japanese journal of infectious diseases","volume":" ","pages":"175-178"},"PeriodicalIF":1.1,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142931719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of Rapid Amplicon-Based Nanopore Sequencing Using The Latest Chemistry for Accurate Whole Genome Analysis of Influenza A Virus in Clinical Samples. 利用最新化学技术对临床样本中甲型流感病毒进行准确全基因组分析的快速扩增子纳米孔测序评估。

IF 1.1 4区医学

Japanese journal of infectious diseases Pub Date : 2025-09-19 Epub Date: 2025-01-31 DOI: 10.7883/yoken.JJID.2024.400

Yumani Kuba, Nobuhiro Takemae, Satoshi Kawato, Kunihiro Oba, Kiyosu Taniguchi, Tsutomu Kageyama

{"title":"Evaluation of Rapid Amplicon-Based Nanopore Sequencing Using The Latest Chemistry for Accurate Whole Genome Analysis of Influenza A Virus in Clinical Samples.","authors":"Yumani Kuba, Nobuhiro Takemae, Satoshi Kawato, Kunihiro Oba, Kiyosu Taniguchi, Tsutomu Kageyama","doi":"10.7883/yoken.JJID.2024.400","DOIUrl":"10.7883/yoken.JJID.2024.400","url":null,"abstract":"MinION sequencing is widely used to sequence influenza A virus (IAV) genomes. However, the accuracy and utility of this approach, which uses the latest chemistry to obtain whole viral genome sequences directly from clinical samples, remain insufficiently investigated. We evaluated the sequencing accuracy of combining simultaneous multi-segment one-step RT-PCR and MinION sequencing using various subtypes of 13 IAV isolates. The latest R10.4.1 chemistry significantly improved sequencing accuracy, achieving ≥99.993% identity with the results of Illumina MiSeq and reducing single nucleotide deletion in homopolymer regions. Applying this method to 11 clinical samples enabled rapid subtype identification and acquisition of eight full-length IAV genomes. In four of these samples, subtype identification of hemagglutinin and neuraminidase was achieved within 20 min of starting the sequencing, and a full-length IAV genome was obtained within 7 h of RNA extraction. However, cross-barcode misassignment during demultiplexing might have affected data interpretation, particularly for samples with low viral genome copy numbers. Although careful data analysis is required for multiplex sequencing of clinical samples with low viral genome copy numbers, this approach can be used for the rapid identification of IAV subtypes and accurate acquisition of full IAV genome sequences from clinical samples.","PeriodicalId":14608,"journal":{"name":"Japanese journal of infectious diseases","volume":" ","pages":"147-155"},"PeriodicalIF":1.1,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143079439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sensitivity Evaluation of a Modified Real-Time Reverse-Transcription PCR Primer to Detect a Measles Virus Variant in Japan in 2024. 改良实时反转录 PCR 引物检测 2024 年日本麻疹病毒变种的灵敏度评估。

IF 1.1 4区医学

Japanese journal of infectious diseases Pub Date : 2025-09-19 Epub Date: 2025-02-28 DOI: 10.7883/yoken.JJID.2024.304

Takako Kurata, Daiki Kanbayashi, Atsushi Kaida, Saeko Morikawa, Satoshi Hiroi, Yuki Hirai, Mei Koyama, Takeshi Miyama, Noriyuki Otsuki, Kazushi Motomura

引用次数: 0

Molecular Detection of Mycobacterium Leprae Using RLEP Loop-Mediated Isothermal Amplification and a Restriction Enzyme to Ensure Amplification Specificity. 使用 RLEP-LAMP 和限制性酶对麻风分枝杆菌进行分子检测，以确保扩增特异性。

IF 1.1 4区医学

Japanese journal of infectious diseases Pub Date : 2025-09-19 Epub Date: 2024-12-27 DOI: 10.7883/yoken.JJID.2024.251

Mukul Sharma, Purna Dwivedi, Srishti Tripathi, Purushottam Patel, Pushpendra Singh

引用次数: 0

Mesocestoides vogae Infection in Dogs: Confusion with Echinococcosis. 犬中皮线虫感染：与棘球蚴病混淆。

IF 1.1 4区医学

Japanese journal of infectious diseases Pub Date : 2025-09-19 Epub Date: 2025-02-28 DOI: 10.7883/yoken.JJID.2024.343

Yasuyuki Morishima, Hiromu Sugiyama

引用次数: 0

Characteristics of Cardiac Injury in Patients Diagnosed with Severe Fever with Thrombocytopenia Syndrome. 发热伴血小板减少综合征患者心脏损伤的特点。

IF 1.1 4区医学

Japanese journal of infectious diseases Pub Date : 2025-09-19 Epub Date: 2025-03-31 DOI: 10.7883/yoken.JJID.2024.254

Jing Sun, Yao Hao, Junying Niu, Zhixiang Du, Jianghua Yang

{"title":"Characteristics of Cardiac Injury in Patients Diagnosed with Severe Fever with Thrombocytopenia Syndrome.","authors":"Jing Sun, Yao Hao, Junying Niu, Zhixiang Du, Jianghua Yang","doi":"10.7883/yoken.JJID.2024.254","DOIUrl":"10.7883/yoken.JJID.2024.254","url":null,"abstract":"This study aimed to characterize cardiac injury in individuals diagnosed with severe fever with thrombocytopenia syndrome (SFTS) and to ascertain its relationship with prognosis. A retrospective analysis of data from 324 patients, diagnosed with SFTS between January 2021 and December 2023, was performed. Patients were categorized into survival and non-survival groups. Univariate and multivariate analyses were performed to identify significant indicators and predictive risk factors for mortality. Statistically significant differences in various parameters were found between the 2 groups, including age, history of hypertension, presence of diarrhea, petechiae, neurological abnormalities, and numerous laboratory measurements. These included lymphocyte, monocyte, and platelet counts, as well as liver enzyme levels, kidney function markers, cardiac biomarkers, clotting factors, inflammatory markers, Dabie Banda virus RNA, heart rate, PR interval, QT interval, and incidence of ST depression. Age, history of hypertension, neurological abnormalities, and creatinine, prothrombin time, and QT intervals were independent risk factors for mortality. The incidence of viral myocarditis in patients with SFTS was 64.12%, and the non-survival group demonstrated a higher incidence of cardiac injury, both earlier and more severe. The incidence of viral myocarditis in patients with SFTS was closely associated with prognosis.","PeriodicalId":14608,"journal":{"name":"Japanese journal of infectious diseases","volume":" ","pages":"156-163"},"PeriodicalIF":1.1,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143752741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multiclonal dissemination of ptxP3 allele macrolide-resistant Bordetella pertussis in Okinawa, Japan. ptxP3等位基因耐大环内酯百日咳在日本冲绳的多克隆传播

IF 1.1 4区医学

Japanese journal of infectious diseases Pub Date : 2025-08-29 DOI: 10.7883/yoken.JJID.2025.123

Tetsuya Kakita, Haruno Taira, Tsuyoshi Kudeken, Megumi Kuniyoshi, Taketoshi Takara, Morimi Teruya, Hisako Kyan, Makoto Ohnishi

引用次数: 0

Evaluating CMV DNA qPCR Assay and Serological Concordance in Diagnosing Congenital CMV Infection in Indonesian Newborns. 评估CMV DNA qPCR检测和血清学一致性诊断印度尼西亚新生儿先天性巨细胞病毒感染。

IF 1.1 4区医学

Japanese journal of infectious diseases Pub Date : 2025-08-29 DOI: 10.7883/yoken.JJID.2025.068

Agustin Iskandar, Brigitta I R V Corebima, Eko Sulistijono, Dina Fauziah

{"title":"Evaluating CMV DNA qPCR Assay and Serological Concordance in Diagnosing Congenital CMV Infection in Indonesian Newborns.","authors":"Agustin Iskandar, Brigitta I R V Corebima, Eko Sulistijono, Dina Fauziah","doi":"10.7883/yoken.JJID.2025.068","DOIUrl":"https://doi.org/10.7883/yoken.JJID.2025.068","url":null,"abstract":"This study investigated the diagnostic accuracy of the quantitative polymerase chain reaction (qPCR) DNA assay in urine and saliva samples, as well as its concordance with serum CMV IgM/IgG testing in infants suspected of congenital cytomegalovirus (cCMV) infection. Furthermore, the study sought to elucidate the correlation between various diagnostic parameters in suspected cases of congenital CMV. A cross-sectional analysis was conducted on newborns suspected of having cCMV infection at RSSA Malang. CMV serology, complete blood count, and qPCR of urine and saliva samples were collected. Statistical analysis was performed on these data with a p-value < 0.05. A significant difference in urine PCR CT value distinguished positive from negative CMV IgM serology groups. A strong correlation (r = 0.728, p < 0.001) and high agreement (κ = 0.693, p < 0.001) between urine and saliva PCR testing suggested saliva as a reliable and non-invasive alternative for newborn screening. The findings of this study underscore the necessity of a comprehensive screening protocol for cCMV, particularly considering the high seroprevalence of CMV in the Indonesian population.","PeriodicalId":14608,"journal":{"name":"Japanese journal of infectious diseases","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144954662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel image-based titration method for potency testing of diphtheria toxoid in Vero cell assay. 基于图像的白喉类毒素Vero细胞效价检测新方法。

IF 1.1 4区医学

Japanese journal of infectious diseases Pub Date : 2025-08-29 DOI: 10.7883/yoken.JJID.2025.030

Miyuki Kimura, Masaaki Iwaki, Tsuyoshi Kenri, Mitsutoshi Senoh

引用次数: 0