Japanese journal of infectious diseases最新文献

A Cross-sectional Study on Faecal Calprotectin Levels on 2-18 Years old HIV-Infected and Healthy Children in Surabaya, Indonesia. 印度尼西亚泗水2-18岁艾滋病毒感染和健康儿童粪便钙保护蛋白水平的横断面研究

IF 1.1 4区医学

Japanese journal of infectious diseases Pub Date : 2026-09-30 DOI: 10.7883/yoken.JJID.2025.193

Alpha Fardah Athiyyah, Andy Darma, Khadijah Rizky Sumitro, Subijanto Marto Sudarmo

{"title":"A Cross-sectional Study on Faecal Calprotectin Levels on 2-18 Years old HIV-Infected and Healthy Children in Surabaya, Indonesia.","authors":"Alpha Fardah Athiyyah, Andy Darma, Khadijah Rizky Sumitro, Subijanto Marto Sudarmo","doi":"10.7883/yoken.JJID.2025.193","DOIUrl":"https://doi.org/10.7883/yoken.JJID.2025.193","url":null,"abstract":"Gastrointestinal manifestation is the most common complication in HIV-infected patient especially enteropathy. Faecal calprotectin is applicable to gut inflammatory biomarker in general population while the sensitivity in HIV-infected patient is remained unknown. This cross-sectional study evaluated between HIV-infected children and control group with the age of 2-18 years old in Dr. Soetomo General Hospital, Surabaya, Indonesia, 19 and 27 samples respectively. This study conducted with collecting stool sample from previous randomized controlled trial using Epitope Cat. No. 30356. The data analysed using Mann-Whitney as non-parametric test and significant with p-value < 0.05. Most of the HIV infected patients are on 1st therapy regimen (78.9%) and all of them are on therapy more than 1 year duration. The mean and standard deviation of the concentration of CD4, percentage of CD4, and ratio of CD4/CD8 were 1,042.47 ± 450.38, 26.01 ± 9.24, and 0.71 ± 0.43, respectively. The median faecal calprotectin level in the HIV-infected group was 63.50 ng/ml (IQR: 21.50 - 203.50), compared to 17.00 ng/ml (IQR: 13.00 - 33.00) (p = 0.004). Faecal calprotectin is significantly higher in HIV-infected patients compared to the control group. Hence, faecal calprotectin can be used as a gut inflammatory biomarker especially in HIV-infected patients.","PeriodicalId":14608,"journal":{"name":"Japanese journal of infectious diseases","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2026-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149109730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of novel human monoclonal antibodies for botulinum neurotoxin type F subtype 1. 新型肉毒杆菌神经毒素F亚型1人单克隆抗体的研制。

IF 1.1 4区医学

Japanese journal of infectious diseases Pub Date : 2026-09-30 DOI: 10.7883/yoken.JJID.2026.105

Ayuha Miyazaki, Seiji Tarumizu, Takayuki Nakajima, Jun Katahira, Tomoko Kohda

{"title":"Development of novel human monoclonal antibodies for botulinum neurotoxin type F subtype 1.","authors":"Ayuha Miyazaki, Seiji Tarumizu, Takayuki Nakajima, Jun Katahira, Tomoko Kohda","doi":"10.7883/yoken.JJID.2026.105","DOIUrl":"https://doi.org/10.7883/yoken.JJID.2026.105","url":null,"abstract":"Different types of Botulinum neurotoxins (BoNTs) cause human botulism. Among them, type F botulism, caused by genetically diverse BoNT/F subtypes, is rare and may be overlooked despite its clinical importance. Toxin-neutralizing human antibodies, which carry the minimum risks of inducing serum sickness or anaphylaxis, are ideal for the treatment of human botulism. In this study, we generated human monoclonal antibodies (mAb) against BoNT/F using trans-chromosomic monoclonal antibody (TC-mAb) mice. A recombinant toxin fragment consisting of the light chain and the heavy chain N-terminal domain (L-HN) of BoNT/F subtype 1 was selected as the antigen because this subtype is used as the reference antigen for currently available antitoxin preparations. Three mAbs (126, 134, and 146) were obtained and reconstructed in a human IgG format. Although the mAbs did not significantly inhibit VAMP2 cleavage in primary cultured neurons, they exhibited partial neutralizing activities in mouse bioassays. In addition, we found that the combinatorial use of 126 and 146 enhanced neutralizing activity. These findings provide important information for the future development of human therapeutic mAbs against BoNT/F.","PeriodicalId":14608,"journal":{"name":"Japanese journal of infectious diseases","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2026-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149109774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Long-Term Dynamics of Neutralizing Antibodies after mRNA COVID-19 Vaccination in Psychiatric Inpatients Following an Outbreak. 爆发后精神科住院患者接种mRNA COVID-19疫苗后中和抗体的长期动态

IF 1.1 4区医学

Japanese journal of infectious diseases Pub Date : 2026-09-30 DOI: 10.7883/yoken.JJID.2026.065

Shinji Nakase, Hajime Kusuhara, Shinichi Kanehara, Hitoshi Hirano, Yasuyuki Hara, Tomoyuki Uno, Kiyosu Taniguchi, Katsuhiro Komase, Hajime Kamiya

{"title":"Long-Term Dynamics of Neutralizing Antibodies after mRNA COVID-19 Vaccination in Psychiatric Inpatients Following an Outbreak.","authors":"Shinji Nakase, Hajime Kusuhara, Shinichi Kanehara, Hitoshi Hirano, Yasuyuki Hara, Tomoyuki Uno, Kiyosu Taniguchi, Katsuhiro Komase, Hajime Kamiya","doi":"10.7883/yoken.JJID.2026.065","DOIUrl":"https://doi.org/10.7883/yoken.JJID.2026.065","url":null,"abstract":"Patients with mental illnesses are more susceptible to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection and are more likely to develop severe disease when infected. Therefore, in many countries, they have been considered a priority group for vaccination. However, the immunological effects of vaccination in these patients remain insufficiently studied. In this study, we investigated changes in serum neutralizing antibody titers against SARS-CoV-2 over a two-year period. Fifty-three participants who had experienced a coronavirus infection (COVID-19) outbreak in a closed psychiatric ward in September 2020 were followed. Antibody titers were measured after natural infection and subsequent vaccination. Neutralizing antibody titers declined over time, and 20% of patients fell below the positivity threshold within six months after infection. In contrast, all patients who received two subsequent mRNA vaccinations maintained high antibody titers for at least six months after vaccination. These findings suggest that vaccination provides effective and sustained humoral immunity in psychiatric patients with a history of COVID-19 infection.","PeriodicalId":14608,"journal":{"name":"Japanese journal of infectious diseases","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2026-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149109777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hypothesis: Transmission of COVID-19 through the Air from Asymptomatic Individuals by Speaking. 假设：无症状者通过言语经空气传播。

IF 1.1 4区医学

Japanese journal of infectious diseases Pub Date : 2026-09-17 Epub Date: 2025-11-28 DOI: 10.7883/yoken.JJID.2025.169

Sakae Inouye

{"title":"Hypothesis: Transmission of COVID-19 through the Air from Asymptomatic Individuals by Speaking.","authors":"Sakae Inouye","doi":"10.7883/yoken.JJID.2025.169","DOIUrl":"10.7883/yoken.JJID.2025.169","url":null,"abstract":"COVID-19 mortality seems to be higher in high-income, hygienic countries, but this is contrary to conventional communicable diseases, which usually occur in low-income countries. Another characteristic of COVID-19 is that a proportion of infected individuals had no symptoms but transmitted the virus to others. In this paper, the author proposes a hypothesis that infected but asymptomatic individuals produce two kinds of infectious respiratory particles by speaking: large droplets and small aerosols. The large droplets are produced from the saliva by pronouncing a plosive, /p/ or /t/, whereas the small aerosols are produced from the laryngeal secretion by the vibration of vocal cords during the voicing of vowels. The heavy droplets collide with other people's faces in conversation, resulting in droplet infection. In contrast, the lighter aerosols float in the air, accumulate inside a non-ventilated room after prolonged conversation, and cause airborne infection in other people in the same room. Then, the author discusses the global epidemiology of COVID-19, which may have depended on 1) climate and housing structure, 2) cultural behaviors, and 3) languages among different countries.","PeriodicalId":14608,"journal":{"name":"Japanese journal of infectious diseases","volume":" ","pages":"218-221"},"PeriodicalIF":1.1,"publicationDate":"2026-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145648500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Human Bocavirus Infection in Turkish Children. 土耳其儿童的人类博卡病毒感染。

IF 1.1 4区医学

Japanese journal of infectious diseases Pub Date : 2026-09-17 Epub Date: 2025-12-26 DOI: 10.7883/yoken.JJID.2025.200

Begüm Murt, Esra Çakmak Taşkın, Halil Özdemir, Tanıl Kendirli, Zeynep Ceren Karahan, Erdal İnce, Ergin Çiftçi

{"title":"Human Bocavirus Infection in Turkish Children.","authors":"Begüm Murt, Esra Çakmak Taşkın, Halil Özdemir, Tanıl Kendirli, Zeynep Ceren Karahan, Erdal İnce, Ergin Çiftçi","doi":"10.7883/yoken.JJID.2025.200","DOIUrl":"10.7883/yoken.JJID.2025.200","url":null,"abstract":"The human bocavirus (HBoV) in children has been identified with an acute lower respiratory tract disease (LRTD). Its prevalence, clinical characteristics, and function as an RTD causal agent are unclear. This study sought to evaluate the laboratory and clinical data of HBoV patients, pinpoint the clinical characteristics linked to HBoV, and examine the distinctions between detecting HBoV alone and in combination with other agents. We used molecular testing to confirm the presence of HBoV DNA and 20 other respiratory pathogens in 1,739 nasopharyngeal samples from hospitalized children at the Pediatric Infectious Diseases Service. 73 children, whose median age was 15 months, had HBoV. Concomitant infection of Bocavirus with another virus was observed in 41 (56.1%) of the patients. Co-infections were observed most frequently with rhinovirus (n = 17) and respiratory syncytial virus (n = 10). There was no difference between the isolated bocavirus and co-infection groups in terms of age, clinical presentations, chest X-ray findings, or laboratory results. The role of HBoV in RTD is supported by the fact that the majority of patients were admitted to the hospital with a diagnosis of pneumonia or bronchopneumonia. HBoV mainly affects the respiratory tract, but it can also cause different clinical manifestations.","PeriodicalId":14608,"journal":{"name":"Japanese journal of infectious diseases","volume":" ","pages":"199-206"},"PeriodicalIF":1.1,"publicationDate":"2026-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145900333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association of Hospital- and Patient-Level Characteristics with SARS-CoV-2 Infection and COVID-19 Mortality Among Patients in Korean Long-Term Care Hospitals: A Multilevel Analysis. 韩国长期护理医院患者SARS-CoV-2感染与COVID-19死亡率的医院和患者水平相关性：一项多水平分析

IF 1.1 4区医学

Japanese journal of infectious diseases Pub Date : 2026-09-17 Epub Date: 2025-12-26 DOI: 10.7883/yoken.JJID.2025.063

Sungwon Lim, Jeong-Yeon Seon, So-Youn Park, Rugyeom Lee, Keomji Go, In-Hwan Oh

{"title":"Association of Hospital- and Patient-Level Characteristics with SARS-CoV-2 Infection and COVID-19 Mortality Among Patients in Korean Long-Term Care Hospitals: A Multilevel Analysis.","authors":"Sungwon Lim, Jeong-Yeon Seon, So-Youn Park, Rugyeom Lee, Keomji Go, In-Hwan Oh","doi":"10.7883/yoken.JJID.2025.063","DOIUrl":"10.7883/yoken.JJID.2025.063","url":null,"abstract":"The Coronavirus disease 2019 (COVID-19) pandemic has disproportionately affected vulnerable populations in long-term care hospitals (LTCHs). This study examines the interplay between patient and hospital factors in relation to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection and COVID-19 mortality in Korean LTCHs. This retrospective cohort study included 262,221 patients (aged ≥60) who were hospitalized for >30 days in 2021. Multilevel logistic regression models were used to assess the association of hospital and patient factors with SARS-CoV-2 infection and COVID-19 mortality. At the hospital level, a higher number of doctors was associated with a decreased risk of SARS-CoV-2 infection (odds ratio [OR] = 0.84, 95% confidence interval [CI]: 0.73-0.98) and COVID-19 mortality (OR = 0.85, 95% CI: 0.78-0.93), whereas nurses and nurse aides were associated with an increased risk of SARS-CoV-2 infection (OR = 1.02, 95% CI: 1.00-1.03) and COVID-19 mortality (OR = 1.01, 95% CI: 1.00-1.02). Among patient-level factors, female sex (infection OR = 0.89, 95% CI: 0.83-0.96; mortality OR = 0.65, 95% CI: 0.55-0.77) was associated with decreased risks of both infection and mortality. These findings highlight the importance of staffing and infection control measures to mitigate COVID-19 risk in LTCH settings.","PeriodicalId":14608,"journal":{"name":"Japanese journal of infectious diseases","volume":" ","pages":"191-198"},"PeriodicalIF":1.1,"publicationDate":"2026-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145900277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of Macrolide-Resistant Mycoplasma Pneumoniae in a Pediatric Primary and Secondary Emergency Care Center in Japan Following the COVID-19 Pandemic. COVID-19大流行后日本儿科一级和二级急救中心耐大环内酯肺炎支原体的检测

IF 1.1 4区医学

Japanese journal of infectious diseases Pub Date : 2026-09-17 Epub Date: 2026-01-30 DOI: 10.7883/yoken.JJID.2025.222

Atsushi Miyake, Kouki Ota, Kenji Gotoh, Kiyohito Okumiya, Tatsuki Mizuochi, Shuji Kuga

{"title":"Detection of Macrolide-Resistant Mycoplasma Pneumoniae in a Pediatric Primary and Secondary Emergency Care Center in Japan Following the COVID-19 Pandemic.","authors":"Atsushi Miyake, Kouki Ota, Kenji Gotoh, Kiyohito Okumiya, Tatsuki Mizuochi, Shuji Kuga","doi":"10.7883/yoken.JJID.2025.222","DOIUrl":"10.7883/yoken.JJID.2025.222","url":null,"abstract":"During the COVID-19 pandemic, Mycoplasma pneumoniae infections were rarely observed in Japan, but a significant outbreak occurred in 2024. At Oita Children's Hospital, a rapid quenching probe polymerase chain reaction (QP-PCR) method was employed to detect the pathogen and to determine the prevalence of macrolide resistance. Between April 1 and September 30, 2024, we tested 679 patients - 462 (68.0%) were positive and 399 (86.4%) were macrolide resistant. The resistance rate was among the highest reported in Japan and comparable to those in neighboring Asian countries. Inpatients were younger than outpatients, and their diagnoses required longer periods of time; corticosteroids were used in 86% of hospitalized cases. QP-PCR detects common A2063G and A2064G mutations and provides results within 40 minutes, which could promote timely therapy, and this is true particularly in the case of younger children, whose options could be limited. Early administration of effective antimicrobial agents is crucial for the control of macrolide-resistant M. pneumoniae. These findings indicate that QP-PCR-based diagnostics could significantly contribute to the management and control of M. pneumoniae outbreaks.","PeriodicalId":14608,"journal":{"name":"Japanese journal of infectious diseases","volume":" ","pages":"214-217"},"PeriodicalIF":1.1,"publicationDate":"2026-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146100066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic Characterization of Enteroviruses Associated with Hand, Foot, and Mouth Disease in Yunnan Province, China, 2023. 云南省手足口病相关肠道病毒的遗传特征，中国，2023

IF 1.1 4区医学

Japanese journal of infectious diseases Pub Date : 2026-09-17 Epub Date: 2025-12-26 DOI: 10.7883/yoken.JJID.2025.172

Xuanguoer Liu, Jianping Cun, Lili Jiang, Xiaoqing Fu, Wenpeng Gu, Hiromu Yoshida, Bingjun Tian

引用次数: 0

Trends in Group A Streptococcus Isolation and Antimicrobial Susceptibility in a Pediatric Population (2021-2023): Comparison with Six Previous Surveys. 2021-2023年儿科人群A群链球菌分离和抗菌药物敏感性趋势：与以往六项调查的比较

IF 1.1 4区医学

Japanese journal of infectious diseases Pub Date : 2026-09-17 Epub Date: 2025-12-26 DOI: 10.7883/yoken.JJID.2025.177

Masanori Sugiura, Naoko Nishimura, Kensei Gotoh, Haruna Kuriyama, Yotaro Kondo, Koji Takemoto, Takao Ozaki

{"title":"Trends in Group A Streptococcus Isolation and Antimicrobial Susceptibility in a Pediatric Population (2021-2023): Comparison with Six Previous Surveys.","authors":"Masanori Sugiura, Naoko Nishimura, Kensei Gotoh, Haruna Kuriyama, Yotaro Kondo, Koji Takemoto, Takao Ozaki","doi":"10.7883/yoken.JJID.2025.177","DOIUrl":"10.7883/yoken.JJID.2025.177","url":null,"abstract":"Group A Streptococcus (GAS) is a major pathogen of respiratory infections in children. This study investigated recent trends in antimicrobial susceptibility among GAS isolates in our hospital and compared them with past data. Between 2021 and 2023, 195 GAS isolates were obtained from 195 pediatric patients. Minimum inhibitory concentrations (MICs) of 22 antimicrobial agents were determined using the broth microdilution method. Susceptibility was categorized as susceptible (S), intermediate (I), or resistant (R) according to the Clinical and Laboratory Standards Institute criteria and compared with six previous surveys (1996-2018; total 1,871 isolates). The number of isolates was low in 2021-2022 but increased sharply in 2023 (n = 157). Most isolates were from throat swabs, and the median age was 5 years (range: 1-15 years). Resistance (I + R) rates were as follows: azithromycin (20%), erythromycin (19%), clarithromycin (19%), tetracycline (8%), and clindamycin (4%). Erythromycin resistance increased from 9% in 1996 to 58% in 2013 but declined to 19% in this study. No β-lactam resistance was previously observed; however, two isolates (1%) showed elevated MICs to cefalexin, suggesting reduced susceptibility. These findings underscore the importance of continued monitoring of GAS isolation trends and antimicrobial resistance, including emerging β-lactam reduced susceptibility.","PeriodicalId":14608,"journal":{"name":"Japanese journal of infectious diseases","volume":" ","pages":"185-190"},"PeriodicalIF":1.1,"publicationDate":"2026-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145900343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinical Evaluation of a Commercial Real-Time PCR Kit for Diagnosis of Acute Dengue Infection. 用于诊断急性登革热感染的商用实时PCR试剂盒的临床评价。

IF 1.1 4区医学

Japanese journal of infectious diseases Pub Date : 2026-09-17 Epub Date: 2026-01-30 DOI: 10.7883/yoken.JJID.2025.150

Premkumar Jagadeeshan, Arpita Maladakar, Lakshmi Sahu, Priya Kumari, Vijayalakshmi Reddy, Lonika Lodha, Reeta Subramaniam Mani

{"title":"Clinical Evaluation of a Commercial Real-Time PCR Kit for Diagnosis of Acute Dengue Infection.","authors":"Premkumar Jagadeeshan, Arpita Maladakar, Lakshmi Sahu, Priya Kumari, Vijayalakshmi Reddy, Lonika Lodha, Reeta Subramaniam Mani","doi":"10.7883/yoken.JJID.2025.150","DOIUrl":"10.7883/yoken.JJID.2025.150","url":null,"abstract":"Dengue virus (DENV), a mosquito-borne flavivirus, has emerged as a major global public health concern, with outbreaks occurring with increasing frequency worldwide, including in India. Laboratory confirmation is essential for accurate diagnosis, effective clinical management, and timely public health response. Real-time PCR (RT-PCR) is recognized as a sensitive and specific method for early diagnosis. This study evaluated the performance of the NeoDX Dengue Virus Screening Real-Time PCR Kit (NeoDX, Bengaluru, India), a pan-DENV assay, against the Centers for Disease Control and Prevention (CDC) DENV-1-4 RT-PCR assay. RNA extracted from 51 sera, obtained from suspected dengue cases, was tested by both assays. Of the 51 samples, 23 were positive by the CDC assay (DENV-1: 6, DENV-2: 4, DENV-3: 10, DEN-4: 3). The NeoDX Dengue Virus Screening Real-Time PCR detected 24 positive samples, including all 23 positive samples by CDC assay, yielding a sensitivity of 100% and specificity of 96.4%. The kappa value (0.961; 95% confidence interval: 0.88-1.0) indicated very good agreement, supporting NeoDX Dengue Virus Screening Real-Time PCR as a reliable assay for early DENV detection.","PeriodicalId":14608,"journal":{"name":"Japanese journal of infectious diseases","volume":" ","pages":"211-213"},"PeriodicalIF":1.1,"publicationDate":"2026-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146100052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0