Japanese journal of infectious diseases最新文献

SARS-CoV-2 Spike-specific T cell responses following COVID-19 vaccination in Japanese people living with HIV. 日本艾滋病毒感染者接种COVID-19疫苗后的SARS-CoV-2刺突特异性T细胞反应

IF 1.3 4区医学

Japanese journal of infectious diseases Pub Date : 2025-06-30 DOI: 10.7883/yoken.JJID.2025.086

Mark Ndubi, Mako Toyoda, Isaac Ngare, Chihiro Motozono, Rumi Minami, Takamasa Ueno

{"title":"SARS-CoV-2 Spike-specific T cell responses following COVID-19 vaccination in Japanese people living with HIV.","authors":"Mark Ndubi, Mako Toyoda, Isaac Ngare, Chihiro Motozono, Rumi Minami, Takamasa Ueno","doi":"10.7883/yoken.JJID.2025.086","DOIUrl":"https://doi.org/10.7883/yoken.JJID.2025.086","url":null,"abstract":"Incompletely resolved immune dysfunction in people living with HIV (PLWH) on antiretroviral treatment (ART) could differentially impact CD4+ and CD8+ T cell subsets. In this study, we investigated SARS-CoV-2 vaccine-induced CD4+ and CD8+ T cell responses in 26 PLWH on ART following third-dose mRNA vaccination. Spike-specific CD4+ and CD8+ T cell responses were assessed based on the expression of activation markers, CD137/OX40 and CD137/CD25, respectively, in response to stimulation with overlapping peptides spanning the spike protein. All participants showed spike-specific T cell responses, with CD8+ responses at a higher median frequency than CD4+. Interestingly, 5 participants who showed a higher frequency of spike-specific CD4+, relative to CD8+ T cells, were significantly younger and had higher CD4 counts pre-ART, in comparison to other participants. Further multivariate analysis revealed that only CD4 count pre-ART was an important predictor of elevated spike-specific CD4+ T cell responses; whereas no association was observed with neutralizing antibody (nAb) potency towards SARS-CoV-2 spike. Our results highlight heterogeneous immune functionality of vaccine-induced, SARS-CoV-2 spike-specific CD4+ and CD8+ T cells in PLHW on ART.","PeriodicalId":14608,"journal":{"name":"Japanese journal of infectious diseases","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Variability in single radial immunodiffusion (SRID) potency affected by influenza vaccine reference antigen/antiserum combinations: relationship between dissociation constant and robustness of SRID potency. 流感疫苗参考抗原/抗血清组合对单一径向免疫扩散（SRID）效力影响的变异性：解离常数与SRID效力稳健性的关系

IF 1.3 4区医学

Japanese journal of infectious diseases Pub Date : 2025-06-30 DOI: 10.7883/yoken.JJID.2024.196

Haruna Nishijima, Noriko Shimasaki, Tomoko Kuwahara, Yusuke Nakai, Kazuya Nakamura, Kayoko Sato, Keiko Murano, Shigeyuki Itamura, Akihide Ryo, Yuichi Harada

{"title":"Variability in single radial immunodiffusion (SRID) potency affected by influenza vaccine reference antigen/antiserum combinations: relationship between dissociation constant and robustness of SRID potency.","authors":"Haruna Nishijima, Noriko Shimasaki, Tomoko Kuwahara, Yusuke Nakai, Kazuya Nakamura, Kayoko Sato, Keiko Murano, Shigeyuki Itamura, Akihide Ryo, Yuichi Harada","doi":"10.7883/yoken.JJID.2024.196","DOIUrl":"https://doi.org/10.7883/yoken.JJID.2024.196","url":null,"abstract":"The potencies of domestic influenza virus reference antigens were initially calibrated using a single radial immunodiffusion (SRID) assay using primarily prepared international reference antigens. The SRID potency should not be affected when using another reference antigen calibrated with the same international antigen. However, the SRID potency of the test antigens can vary, although the causes of these discrepancies remain unclear. Here, we calibrated two candidate reference antigens (LotA and LotB) in the A(H3N2) subtype with various pairs of reference reagent sets (antigen and antiserum). The potencies of LotA and LotB varied depending on the reagent pair used, with a more pronounced effect in LotA (CV = 5.4% vs. 3.8%). To explore the cause of these divergences, we analyzed the dissociation constant of each reagent pair and scored them based on the hypothesis that pairs exhibiting stronger antigen-antibody binding would have smaller precipitin rings. Comparing these scores with the respective potency scores, we observed a strong correlation between the Binding score (relative BLI-KD) and potency score in LotA (r = 0.8464, p = 0.0001) but not in LotB (r = 0.4000, p = 0.1408). These data suggest that antigen-antibody binding strength is an influencing factor of SRID potency.","PeriodicalId":14608,"journal":{"name":"Japanese journal of infectious diseases","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RNA virome analysis of Haemaphysalis longicornis ticks collected in Hyogo, Japan. 日本兵库县长角血蜱RNA病毒组分析。

IF 1.3 4区医学

Japanese journal of infectious diseases Pub Date : 2025-06-30 DOI: 10.7883/yoken.JJID.2025.027

Alisa Rose Aboshi, Ryo Matsumura, Mizue Inumaru, Kyoko Sawabe, Mamoru Watanabe, Toshinori Sasaki, Daisuke Kobayashi, Kyo Itoyama, Haruhiko Isawa

{"title":"RNA virome analysis of Haemaphysalis longicornis ticks collected in Hyogo, Japan.","authors":"Alisa Rose Aboshi, Ryo Matsumura, Mizue Inumaru, Kyoko Sawabe, Mamoru Watanabe, Toshinori Sasaki, Daisuke Kobayashi, Kyo Itoyama, Haruhiko Isawa","doi":"10.7883/yoken.JJID.2025.027","DOIUrl":"https://doi.org/10.7883/yoken.JJID.2025.027","url":null,"abstract":"Tick-borne viruses are primarily transmitted to vertebrates by infected ticks during blood feeding and cause various diseases in humans and animals. Haemaphysalis longicornis is one of the main tick species responsible for human tick bites and is thought to be the primary vector of severe fever with thrombocytopenia syndrome, an important tick-borne viral disease in Japan. Although H. longicornis ticks pose a potential risk to humans in Japan, studies on tick-borne viral prevalence of this tick species in Japan are limited. In this study, we conducted RNA virome analysis of H. longicornis ticks collected in Toyooka City, Hyogo Prefecture, Japan. Two known viruses, Dabieshan tick virus and Hubei sobemo-like virus 15, and putative novel quaranjavirus-like sequences were detected. Additionally, assessments of endogenous viral elements (EVEs) related to the virus and virus-like sequences suggested putative novel quaranjavirus-like sequences existed in both RNA and DNA forms. However, we could not determine whether this quaranjavirus-like sequence was of viral origin and could not conclude whether the DNA forms of the quaranjavirus-like sequence existed as EVEs in ticks. This study provides new insights into the prevalence of tick-associated viruses in ixodid ticks and serves as a reference for future approaches to prevent tick-borne diseases.","PeriodicalId":14608,"journal":{"name":"Japanese journal of infectious diseases","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144527999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Epidemiological Predisposing Factors Associated with Bacterial Necrotizing Fasciitis in Rural Thailand: A Case-Control Study. 泰国农村细菌性坏死性筋膜炎相关的流行病学易感因素：一项病例对照研究。

IF 1.3 4区医学

Japanese journal of infectious diseases Pub Date : 2025-06-30 DOI: 10.7883/yoken.JJID.2024.387

Praewpan Polyiam, Naowarut Wangnadee, Benjawan Comhangpol, Weerawat Labnongsaeng, Nuntiput Putthanachote, Ratchadaporn Ungcharoen, Anusak Kerdsin

{"title":"Epidemiological Predisposing Factors Associated with Bacterial Necrotizing Fasciitis in Rural Thailand: A Case-Control Study.","authors":"Praewpan Polyiam, Naowarut Wangnadee, Benjawan Comhangpol, Weerawat Labnongsaeng, Nuntiput Putthanachote, Ratchadaporn Ungcharoen, Anusak Kerdsin","doi":"10.7883/yoken.JJID.2024.387","DOIUrl":"https://doi.org/10.7883/yoken.JJID.2024.387","url":null,"abstract":"Necrotizing fasciitis (NF) is rapidly progressive soft tissue infection with a high mortality rate. Identifying the predisposing factors for NF is critical for enabling diagnosis and medical treatment. This study aimed to identify the factors associated with NF in a hospital located in rural Thailand. In total, 167 NF and 147 cellulitis cases were compared between 2019 and 2021. Among the NF cases, 13.17% required amputation and 7.69% were fatal. The study identified male sex, farming occupation, S. pyogenes infection, and comorbidities, including diabetes mellitus (DM), chronic kidney disease, and hypertension, as significant predisposing factors for NF. DM was statistically significantly associated with an increased risk of amputation. For pathogen-specific NF, advanced age (≥ 60 years) was the only statistically significant predisposing factor for S. pyogenes-associated NF. Conversely, NF caused by Staphylococcus aureus is significantly associated with farming occupation, DM, and hypertension. For NF caused by Enterobacterales, male sex, farming occupation, DM, and chronic kidney disease were statistically significant predisposing factors. These may not be risk factors that can be generalized, but may be the only risk factors for NF in Thailand. However, these findings may inform the development of guidelines for nursing care and pathogen-specific prevention of NF.","PeriodicalId":14608,"journal":{"name":"Japanese journal of infectious diseases","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144527998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mpox in an immunocompromised patient: Fatal case report, Colombia. 一例免疫功能低下患者的麻疹：致命病例报告，哥伦比亚。

IF 1.3 4区医学

Japanese journal of infectious diseases Pub Date : 2025-05-30 DOI: 10.7883/yoken.JJID.2024.120

Sheryll Corchuelo Chavarro, Alicia Rosales Munar, Edgar Parra Saad, Cristian Arbey Velarde Hoyos, Idabely Betancur Ortiz, Orlando Torres-Fernandez, Lady Rivera, Diana Walteros, Franklyn Prieto, Marcela Mercado

{"title":"Mpox in an immunocompromised patient: Fatal case report, Colombia.","authors":"Sheryll Corchuelo Chavarro, Alicia Rosales Munar, Edgar Parra Saad, Cristian Arbey Velarde Hoyos, Idabely Betancur Ortiz, Orlando Torres-Fernandez, Lady Rivera, Diana Walteros, Franklyn Prieto, Marcela Mercado","doi":"10.7883/yoken.JJID.2024.120","DOIUrl":"https://doi.org/10.7883/yoken.JJID.2024.120","url":null,"abstract":"Mpox is a viral zoonosis caused by the monkeypox virus (MPXV), a double-stranded DNA virus mostly transmitted by direct contact, respiratory droplets, and contaminated fomites. Immunodeficiency, younger age, chronic diseases, and lack of immunization are all associated with severe illness. Mpox was declared by the World Health Organization a Public Health Emergency of International Concern on May 20, 2022. This report aimed to provide a detailed histopathological and immunohistochemical findings of a fatal case of an immunosuppressed patient infected with MPXV, reported in Colombia in September 2022. A description of the clinical findings was made, followed by histopathological, immunohistochemical, and molecular studies to confirm the presence of MPXV genomes and viral antigens in different tissues. MPXV viral DNA of the clade IIb was identified, and MPXV genomes were found in the liver, lung, heart, and brain. Interestingly, MPXV antigens were observed in the skin and lung, mainly in necrotic areas surrounded by active inflammatory cell markers. The simultaneous use of several diagnostic tools, as histopathological, molecular and Next Generation Sequencing in fatal cases involving a variety of viral agents, provide relevant information to understand the pathogenesis and clarify the cause of death of this emerging infectious disease.","PeriodicalId":14608,"journal":{"name":"Japanese journal of infectious diseases","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144199144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Report on the Measles Outbreak Case in a Medical Institution in South Korea, 2024. 2024年韩国某医疗机构麻疹暴发病例报告

IF 1.3 4区医学

Japanese journal of infectious diseases Pub Date : 2025-05-30 DOI: 10.7883/yoken.JJID.2024.207

Soo Hyeon Cho, Eun Jung Jang, Joon Jai Kim, Mi-Gyeong Kim, Seonju Yi, Seung Eun Lee

{"title":"Report on the Measles Outbreak Case in a Medical Institution in South Korea, 2024.","authors":"Soo Hyeon Cho, Eun Jung Jang, Joon Jai Kim, Mi-Gyeong Kim, Seonju Yi, Seung Eun Lee","doi":"10.7883/yoken.JJID.2024.207","DOIUrl":"https://doi.org/10.7883/yoken.JJID.2024.207","url":null,"abstract":"This report analyzes a measles outbreak that occurred at a medical institution (Hospital A) in Gyeonggi-do in April 2024, aiming to examine its epidemiological characteristics and derive implications for disease control policies. A cross-sectional study was conducted on 469 confirmed cases and contacts associated with Hospital A during the outbreak period using telephone interviews and on-site epidemiological investigations. Frequency analysis of epidemiological data was performed using statistical software. The attack rate related to the outbreak at Hospital A was 0.64%, with a secondary attack rate of 25.0%. The outbreak involved three confirmed measles cases, including one patient (index case) and two healthcare workers (secondary cases), all carrying the B3 genotype. In total, 469 individuals were exposed during the outbreak at Hospital A, including the three confirmed cases. The outbreak likely originated from a hospital visitor as the source of infection, with no additional transmission beyond hospital staff. To prevent transmission, prompt contact management, periodic antibody testing, and additional vaccinations for staff are recommended.","PeriodicalId":14608,"journal":{"name":"Japanese journal of infectious diseases","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144199145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An invasive macrolide/lincosamide-resistant Corynebacterium mucifaciens isolate from a patient with diabetic gangrene: Colonies with mucoid appearance harboring a fragment of erm(X). 从糖尿病坏疽患者中分离出的侵袭性大环内酯/利可沙胺耐药黏液棒状杆菌：具有粘液样外观的菌落含有erm(X)片段。

IF 1.3 4区医学

Japanese journal of infectious diseases Pub Date : 2025-05-30 DOI: 10.7883/yoken.JJID.2025.034

Takumi Ebihara, Kyoko Baba, Haruno Yoshida, Yoneji Hirose, Mieko Goto, Takashi Takahashi

{"title":"An invasive macrolide/lincosamide-resistant Corynebacterium mucifaciens isolate from a patient with diabetic gangrene: Colonies with mucoid appearance harboring a fragment of erm(X).","authors":"Takumi Ebihara, Kyoko Baba, Haruno Yoshida, Yoneji Hirose, Mieko Goto, Takashi Takahashi","doi":"10.7883/yoken.JJID.2025.034","DOIUrl":"https://doi.org/10.7883/yoken.JJID.2025.034","url":null,"abstract":"Corynebacterium mucifaciens, usually isolated from sterile human specimens, is a rare Corynebacterium species. We describe a blood-origin C. mucifaciens isolate that was resistant to macrolides/lincosamides and had been isolated from a patient with diabetic gangrene. This isolate formed mucoid colonies harboring a fragment of erm(X). As an initial antimicrobial, piperacillin/tazobactam was intravenously administered to the patient for two weeks. Gangrene debridement resulted in good local management. The clinical course was uneventful. The subculture from blood on a blood agar plate revealed mucoid colonies with a positive string test. Gram staining revealed the presence of a mucoid layer around the coryneform. The minimum inhibitory concentrations determined using broth microdilution method indicated resistance to erythromycin/clindamycin. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS), 16S rRNA gene sequencing, and antimicrobial resistance (AMR) gene profiling were performed. MALDI-TOF-MS identified this isolate as C. mucifaciens based on its high score (2.22). 16S rRNA gene sequencing revealed 99.3% similarity with DMMZ 2278(T) 16S rRNA gene sequence. AMR gene profiling revealed that this isolate possessed a fragment identical to that of erm(X) from Actinotignum schaalii. MALDI-TOF-MS with 16S rRNA gene sequencing can be useful to identify C. mucifaciens when the coryneform shows a mucoid colony appearance with hyperviscosity.","PeriodicalId":14608,"journal":{"name":"Japanese journal of infectious diseases","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144199140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Estimated number of mumps cases based on clinical and laboratory data from sentinel surveillance in Kanazawa, Japan. 根据日本金泽哨点监测的临床和实验室数据估计的腮腺炎病例数。

IF 1.3 4区医学

Japanese journal of infectious diseases Pub Date : 2025-05-30 DOI: 10.7883/yoken.JJID.2024.331

Manami Nakashita, Hajime Kamiya, Reiji Watanabe, Yusuke Kobayashi, Yumani Kuba, Rie Koshida, Eri Nariai, Kiyosu Taniguchi, Shigeru Suga, Eiichi Kodama, Tomimasa Sunagawa, Motoi Suzuki

{"title":"Estimated number of mumps cases based on clinical and laboratory data from sentinel surveillance in Kanazawa, Japan.","authors":"Manami Nakashita, Hajime Kamiya, Reiji Watanabe, Yusuke Kobayashi, Yumani Kuba, Rie Koshida, Eri Nariai, Kiyosu Taniguchi, Shigeru Suga, Eiichi Kodama, Tomimasa Sunagawa, Motoi Suzuki","doi":"10.7883/yoken.JJID.2024.331","DOIUrl":"https://doi.org/10.7883/yoken.JJID.2024.331","url":null,"abstract":"Currently, the mumps vaccine is not routinely recommended in Japan. Adding the mumps vaccine to the routine vaccination program requires an accurate estimation of the mumps viral infection disease burden. However, no precise estimate exists in Japan because mumps surveillance is sentinel surveillance with a reporting definition consisting of only a clinical diagnosis (parotid swelling). Since parotid swelling can be caused by pathogens other than the mumps virus, the estimation of mumps cases using the current surveillance data is inaccurate. To obtain an accurate burden of disease, we estimated the burden of the disease using the test results performed at a single sentinel site for laboratory-based surveillance during the mumps endemic (Week 14, 2015, to Week 13, 2016) in Kanazawa. The estimated number of mumps cases using laboratory-confirmed cases was 3,881 (95% confidence interval: 3,404-4,357), approximately 1,000 cases less than the estimated number using clinically compatible mumps cases. Although the estimated number of mumps cases using laboratory-confirmed cases was less than that of clinically compatible cases, the frequency of hearing loss due to mumps could potentially be 40% higher than that currently reported. To make the argument for routine mumps vaccination, surveillance using diagnostic testing information is important.","PeriodicalId":14608,"journal":{"name":"Japanese journal of infectious diseases","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144199143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Draft genome sequence based genomic characterization of Salmonella enterica subsp. enterica serovar Oslo isolated from clinical samples in South India and its comparison with global isolates. 基于草图基因组序列的肠沙门氏菌亚种基因组鉴定。从南印度临床样本中分离的肠球菌奥斯陆血清型及其与全球分离株的比较。

IF 1.3 4区医学

Japanese journal of infectious diseases Pub Date : 2025-05-30 DOI: 10.7883/yoken.JJID.2024.406

Paulami Dutta, Gourab Halder, Manisha Ghosh, Beena Antony, Priyanka Denny, Arindam Ganai, Shanta Dutta

{"title":"Draft genome sequence based genomic characterization of Salmonella enterica subsp. enterica serovar Oslo isolated from clinical samples in South India and its comparison with global isolates.","authors":"Paulami Dutta, Gourab Halder, Manisha Ghosh, Beena Antony, Priyanka Denny, Arindam Ganai, Shanta Dutta","doi":"10.7883/yoken.JJID.2024.406","DOIUrl":"https://doi.org/10.7883/yoken.JJID.2024.406","url":null,"abstract":"Non-typhoidal Salmonellae (NTS) are one of the significant food-borne pathogens with ≥2600 serovars and diverse range of hosts which significantly increases the chances of infections in humans. Salmonella enterica subsp. enterica serovar Oslo (S. Oslo) is a rare serovar, associated with foodborne illnesses. Here, we aim to report the draft whole genome sequences of S. Oslo ST2344 and ST1370 with reduced ciprofloxacin susceptibility, isolated from clinical samples. Thirty S. Oslo isolates were identified following the standard microbiological techniques. Twenty-six of the thirty (86.66%) S. Oslo isolates were pan-susceptible to the antimicrobials examined, except four isolates, which showed decreased ciprofloxacin susceptibility (MIC 0.12 - 0.50 µg/ml). PFGE analysis showed strains were heterogenous with two sequence types of ST1370 and ST2344 were observed in MLST. The average genome size of the four S. Oslo isolates ranged from 4,760,441 - 5,002,787 bp with average G+C content of 52 %. Different virulence genes encompassing Salmonella Pathogenicity Island (SPI) 1 to 5 linked to disease pathogenesis were found by genome analysis. The draft genome of S. Oslo clinical isolates gives an insight into the molecular characteristics of S. Oslo currently prevalent in India and the phylogenetic analysis sheds light on their evolutionary pattern.","PeriodicalId":14608,"journal":{"name":"Japanese journal of infectious diseases","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144199142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antimicrobial susceptibility and clinical phenotypes of Staphylococcus argenteus isolated from clinical specimens. 临床标本分离的阿根廷葡萄球菌的抗菌敏感性和临床表型。

IF 1.3 4区医学

Japanese journal of infectious diseases Pub Date : 2025-05-30 DOI: 10.7883/yoken.JJID.2025.067

Nobumasa Okumura, Nana Akazawa-Kai, Haruka Tsukamoto, Naoya Itoh

{"title":"Antimicrobial susceptibility and clinical phenotypes of Staphylococcus argenteus isolated from clinical specimens.","authors":"Nobumasa Okumura, Nana Akazawa-Kai, Haruka Tsukamoto, Naoya Itoh","doi":"10.7883/yoken.JJID.2025.067","DOIUrl":"https://doi.org/10.7883/yoken.JJID.2025.067","url":null,"abstract":"Recently, Staphylococcus argenteus, previously indistinguishable from Staphylococcus aureus, has been recognized as a clinically relevant microorganism because of mass spectrometry. However, despite its clinical significance, limited information is available on S. argenteus. This study evaluated the antimicrobial susceptibility and clinical phenotypes of S. argenteus isolated from clinical specimens. This single-center, retrospective study analyzed cases wherein S. argenteus was detected in clinical specimens obtained at Nagoya City University East Medical Center between April 2023 and April 2024. The organism was identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Patient data, including clinical outcomes, were collected and reviewed to classify cases as infections or colonization. S. argenteus was isolated from 23 specimens of 21 patients, with sputum being the most common source (78.3%). Sixty-seven percent of isolates were susceptible to penicillin; 95.2% to erythromycin; and 100% to oxacillin, clindamycin, sulfamethoxazole-trimethoprim, vancomycin, and teicoplanin. Thirteen cases of infection were recorded; pneumonia was the predominant diagnosis (11 cases) in these cases, followed by otitis externa and vertebral osteomyelitis. Most patients were successfully treated with antibiotics, and only one died of S. argenteus pneumonia.","PeriodicalId":14608,"journal":{"name":"Japanese journal of infectious diseases","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144199141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0