Agustin Iskandar, Brigitta I R V Corebima, Eko Sulistijono, Dina Fauziah
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引用次数: 0
摘要
本研究探讨了尿样和唾液标本定量聚合酶链反应(qPCR) DNA检测诊断先天性巨细胞病毒(cCMV)感染婴儿的准确性及其与血清巨细胞病毒IgM/IgG检测的一致性。此外,该研究试图阐明先天性巨细胞病毒疑似病例中各种诊断参数之间的相关性。对马朗地区疑似cCMV感染的新生儿进行了横断面分析。收集巨细胞病毒血清学、全血细胞计数、尿液和唾液样本qPCR。对这些资料进行p值< 0.05的统计学分析。尿PCR CT值的显著差异区分阳性和阴性CMV IgM血清学组。尿液和唾液PCR检测之间的强相关性(r = 0.728, p < 0.001)和高度一致性(κ = 0.693, p < 0.001)表明,唾液是新生儿筛查的可靠且无创的替代方法。这项研究的结果强调了cCMV全面筛查方案的必要性,特别是考虑到印度尼西亚人群中CMV的高血清阳性率。
Evaluating CMV DNA qPCR Assay and Serological Concordance in Diagnosing Congenital CMV Infection in Indonesian Newborns.
This study investigated the diagnostic accuracy of the quantitative polymerase chain reaction (qPCR) DNA assay in urine and saliva samples, as well as its concordance with serum CMV IgM/IgG testing in infants suspected of congenital cytomegalovirus (cCMV) infection. Furthermore, the study sought to elucidate the correlation between various diagnostic parameters in suspected cases of congenital CMV. A cross-sectional analysis was conducted on newborns suspected of having cCMV infection at RSSA Malang. CMV serology, complete blood count, and qPCR of urine and saliva samples were collected. Statistical analysis was performed on these data with a p-value < 0.05. A significant difference in urine PCR CT value distinguished positive from negative CMV IgM serology groups. A strong correlation (r = 0.728, p < 0.001) and high agreement (κ = 0.693, p < 0.001) between urine and saliva PCR testing suggested saliva as a reliable and non-invasive alternative for newborn screening. The findings of this study underscore the necessity of a comprehensive screening protocol for cCMV, particularly considering the high seroprevalence of CMV in the Indonesian population.
期刊介绍:
Japanese Journal of Infectious Diseases (JJID), an official bimonthly publication of National Institute of Infectious Diseases, Japan, publishes papers dealing with basic research on infectious diseases relevant to humans in the fields of bacteriology, virology, mycology, parasitology, medical entomology, vaccinology, and toxinology. Pathology, immunology, biochemistry, and blood safety related to microbial pathogens are among the fields covered. Sections include: original papers, short communications, epidemiological reports, methods, laboratory and epidemiology communications, letters to the editor, and reviews.