Iranian Journal of Biotechnology最新文献

{"title":"<i>MiRNA-145-5p</i> Restrains Malignant Behaviors of Breast Cancer Cells Via Downregulating <i>H2AFX</i> Expression.","authors":"Deshun Yao, Xin Chen","doi":"10.30498/ijb.2023.349450.3433","DOIUrl":"https://doi.org/10.30498/ijb.2023.349450.3433","url":null,"abstract":"<p><p>Breast cancer is a prevalent tumor with high aggressiveness among female populations. <i>MiRNA-145-5p</i> plays an important role in multiple cancers.</p><p><strong>Materials and methods: </strong>qRT-PCR detected <i>miRNA-145-5p</i> and histone protein family member X (<i>H2AFX</i>) mRNA expression in breast cancer cells, and western blot determined the protein expression of H2AFX. After predicting the target genes via the bioinformatics methods, the targeting relationship between <i>miRNA-145-5p</i> and <i>H2AFX</i> was verified by dual-luciferase, RIP, and RNA pull-down assays. The relationship between <i>H2AFX</i> and clinical indexes was also analyzed. Furthermore, the effects of <i>miRNA-145-5p/H2AFX</i> regulatory axis on breast cancer cell progression were determined by colony formation, wound healing, CCK-8, and Transwell assays.</p><p><strong>Results: </strong>The results suggested that miRNA-145-5p was markedly lowly-expressed in breast cancer tissue and cells, while <i>H2AFX</i> was upregulated, which had a positive correlation with T stages of breast cancer. Besides, overexpressed <i>miRNA-145-5p</i> was found to remarkably suppress progression of breast cancer cells. As bioinformatic analysis predicted that <i>H2AFX</i> was the potential target of <i>miRNA-145-5p</i>, the dual-luciferase assay was conducted, which demonstrated that <i>miRNA-145-5p</i> negatively regulated the expression of H2AFX by targeting its 3'-UTR. The rescue experiment demonstrated that overexpression of miRNA-145-5p could offset the promotion effects of oe-H2AFX on malignant progression.</p><p><strong>Objective: </strong>Our study is aimed at exploring how <i>miRNA-145-5p</i> functions in breast cancer cells.</p><p><strong>Conclusion: </strong>Our findings confirmed that <i>miRNA-145-5p</i> hindered malignant progression of breast cancer by negatively regulating <i>H2AFX</i>. <i>MiRNA-145-5p/H2AFX</i> axis may be a novel therapeutic target for breast cancer.</p>","PeriodicalId":14492,"journal":{"name":"Iranian Journal of Biotechnology","volume":"21 3","pages":"e3433"},"PeriodicalIF":1.3,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10858355/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139722523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dexmedetomidine Suppresses Glutamate-Stimulated Neuronal Injury Via MicroRNA-433/Janus Kinase 2/Signal Transducer and Activator of Transcription 3 Pathway.","authors":"Lin Zhao, Junlan Huang, Zhouquan Wu, Guowei Fu","doi":"10.30498/ijb.2023.318052.3214","DOIUrl":"https://doi.org/10.30498/ijb.2023.318052.3214","url":null,"abstract":"<p><strong>Background: </strong>Cerebral ischemia has been a hotpot in the prevention and treatment of cerebral ischemia. Dexmedetomidine (Dex) is a new type of highly selective α2 adrenergic receptor agonist with pharmacological properties.</p><p><strong>Objective: </strong>Quantitative studies have shown that Dex has a protective effect on glutamate (Glu)-induced neuronal damage. however, its mechanism has not been fully elucidated. The purpose of this study was to explore the underlying molecular mechanism by which Dex ameliorates Glu-induced neuronal injury by regulating miR-433/JAK2/STAT3 axis.</p><p><strong>Materials and methods: </strong>A model of neuronal injury was constructed by Glu treatment and intervened with Dex. miRNA expression profiling assay was conducted to screen potential miRNAs affected by Dex. Cell viability, lactate dehydrogenase (LDH) release and apoptosis were detected by MTT assay, LDH kit, and TUNEL staining, respectively. Oxidative stress indicators were assessed by ELISA whereas mitochondrial membrane potential (MMP) was assessed by C11-BODIPY581/591 staining. The targeting relationship between the miR-433 and JAK2 was verified by dual-luciferase reporter assay and gene expression was analyzed by quantitative PCR and Western blot.</p><p><strong>Results: </strong>Glu treatment decreased cell viability and MMP and promoted LDH release, apoptosis and oxidative damage. Glu-induced changes in neurons were reversed after Dex treatment through upregulating the miR-433 expression to block the activation of JAK2/STAT3 pathway.</p><p><strong>Conclusions: </strong>Dex protects against Glu-induced neuronal injury by regulating miR-433/JAK2/STAT3 pathway, which provides new insights into the treatment of neuronal injury.</p>","PeriodicalId":14492,"journal":{"name":"Iranian Journal of Biotechnology","volume":"21 3","pages":"e3214"},"PeriodicalIF":1.3,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10858352/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139722527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of Collagen and Fibrin Hydrogels Encapsulated with Adipose Tissue Mesenchymal Stem Cell-Derived Exosomes for Treatment of Spinal Cord Injury in a Rat Model.","authors":"Zohreh Afsartala, Mahmoudreza Hadjighassem, Sadegh Shirian, Somayeh Ebrahimi-Barough, Leila Gholami, Gilda Parsamanesh, Ziba Veisimalekshahi, Latifeh Karimzadehbardeei, Jafar Ai","doi":"10.30498/ijb.2023.362229.3505","DOIUrl":"https://doi.org/10.30498/ijb.2023.362229.3505","url":null,"abstract":"<p><strong>Background: </strong>Mesenchymal stem cell (MSC) derived exosomes (MSC-DE) have been demonstrated to be potential candidates for the treatment of rat spinal cord injury (SCI).</p><p><strong>Objective: </strong>The effect of AD-MSC and AD-MSC-DE encapsulated into collagen and fibrin hydrogels on the treatment of SCI in a rat animal model was investigated for introducing a new effective SCI treatment method.</p><p><strong>Materials and methods: </strong>The AD-MSC-DE was isolated using ultra-centrifugation at 100,000×g for 120 min and characterized by different methods. Fibrin and collagen hydrogels were synthesized and then mixed with AD-MSC-DE suspension. the characterized AD-MSC-DE were encapsulated into collagen and fibrin hydrogels. eighteen adult male Wister rats were randomly classified into 3 equal groups (n=6): the control group (SCI rat without treatment), SCI rat treated with either AD-MSC-DE encapsulated in collagen hydrogel or encapsulated in fibrin hydrogel groups. the treatment approaches were evaluated using clinical, histological, and molecular assays.</p><p><strong>Results: </strong>The AD-MSC-DE encapsulated into fibrin and collagen groups showed better clinical function than the control group. The AD-MSC-DE encapsulated into fibrin and collagen also improved SCI-induced polio and leuko-myelomalacia and leads to higher expression of NF protein than the control group. In the AD-MSC-DE encapsulated into collagen and fibrin leads to up-regulation the mean levels of NEFL (23.82 and 24.33, respectively), eNOS (24.31 and 24.53, respectively), and CK19 mRNAs (24.23 and 23.98, respectively) compared to the control group.</p><p><strong>Conclusion: </strong>The AD-MSC-DE encapsulated within ECM-based hydrogel scaffolds such as collagen and fibrin can regenerate the injured nerve in SCI rats and reduce spinal cord lesion-induced central neuropathic pain.</p>","PeriodicalId":14492,"journal":{"name":"Iranian Journal of Biotechnology","volume":"21 3","pages":"e3505"},"PeriodicalIF":1.3,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10858360/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139722566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of Affinity Chromatography Based on Sepharose 4B- chitin for Rapid Purification of <i>Urtica dioica</i> Agglutinin.","authors":"Mohammadkazem Heydari, Abasalt Hosseinzadeh Colagar, Davood Sabour","doi":"10.30498/ijb.2023.339309.3364","DOIUrl":"https://doi.org/10.30498/ijb.2023.339309.3364","url":null,"abstract":"<p><strong>Background: </strong>Today, numerous antimicrobial and anticancer properties have been reported for plant lectins due to their ability to bind to carbohydrates. The <i>Urtica dioica</i> agglutinin (UDA lectin) is a monomeric, small, and low molecular weight glycoprotein. It has attracted the attention of many researchers for identification, treatment, and other clinical purposes.</p><p><strong>Objectives: </strong>The aim of this study is the optimization of the chitin affinity chromatography based on Sepharose 4B (CNBr-activated Sepharose 4B) for the rapid purification of UDA lectin from <i>Urtica dioica</i> rhizome.</p><p><strong>Materials and methods: </strong>The chitin ligands were dissolved in 40% Trichloroacetic acid and attached to Sepharose 4B according to the Amersham-Biosciences instructions. The attachment of the ligand to the Sepharose 4B beads was investigated by Fourier transform infrared (FTIR) spectroscopy. An acidic crude extract of nettle rhizome passes from chromatographic columns in two sizes with dimensions: 24 x 0.51 cm and 8.44 x 0.86 cm. Quantity and quality of purified lectin were calculated by the Bradford microplate method: SDS-PAGE gel electrophoresis and human erythrocyte cell (RBC) hemagglutination, respectively.</p><p><strong>Results: </strong>The analysis of FTIR spectrograms showed that major changes were observed in the fingerprint regions. Besides, due to the dissolution of Sepharose 4B and chitin in the aqueous phase, this difference was not significant in the Imine and Nitrile regions. On the other hand, the comparative results of purification chromatograms showed that increasing the column length causes a smaller half-width and increases the length of the purified peak. Also, it leads to high-quality purified UDA lectin, with a molecular weight of almost 12.5 kDa in gel electrophoresis. Hemagglutination activity on trypsinized red blood cells was displayed, and agglutination of purified UDA lectin started at least at 300 μg.mL^-1 concentration.</p><p><strong>Conclusion: </strong>According to our findings, we suggested that dissolving chitin in the polar solvent of Trichloroacetic acid, using Sepharose 4B as the beads of a matrix, and increasing the column length might lead to a decrease in the half-width of the peak. These can increase the purity and concentration of purified UDA lectin, and speed up the purification process. These findings could be used by researchers to accelerate the purification of UDA lectin in other studies, dealing with drug delivery systems, ELISA techniques, and cell growth.</p>","PeriodicalId":14492,"journal":{"name":"Iranian Journal of Biotechnology","volume":"21 3","pages":"e3364"},"PeriodicalIF":1.3,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10858356/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139722528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Data-Mining of Barley to Identify Salt Stress Hub Genes, Gene Expression Analysis and Recombinant Plasmid Construction.","authors":"Ehsan Sohrabi, Masoud Tohidfa, Asadolah Ahmadikhah, Rahele Ghanbari Moheb Seraj","doi":"10.30498/ijb.2023.343700.3389","DOIUrl":"https://doi.org/10.30498/ijb.2023.343700.3389","url":null,"abstract":"<p><strong>Background: </strong>Salinity is one of the major abiotic stresses that limit the production and yields of agricultural crops worldwide.</p><p><strong>Objectives: </strong>In order to identify key barley genes under salinity stress, the available metadata were examined by two methods of Cytoscape and R software. Next, the hub expression of the selected gene was evaluated under different salinity stress treatments and finally, this gene was cloned into cloning and expression vector and recombinant plasmid was made.</p><p><strong>Materials and methods: </strong>In this study, we extracted salinity stress tolerant genes from several kinds of literature and also microarray data related to barley under salinity conditions from various datasets. The list of genes related to literature analyzed using string and Cytoscape. The genes from the datasets were first filtered and then the hub genes were identified by Cytoscape and R methods. Next, these hub genes were analyzed for the promoter.</p><p><strong>Results: </strong>Ten hub genes were selected and their promoters were analyzed, the <i>cis</i>-element of which was often cis-acting regulatory element involved in the methyl jasmonate -responsiveness, common <i>cis</i>-acting element in promoter and enhancer regions and MYBHv1 binding site. Finally, the sedoheptulose-1,7-bisphosp gene (<i>SBPase</i>), which had the highest interaction in both gene lists and both types of gene networks, was selected as hub gene. Next, the expression of <i>SBPase</i> gene was examined in two variety of Youssef variety (salt tolerant) and Fajr variety (salt sensitive) under salinity stress (NaCl 100mM) at 0 (control), 3, 6, 12 and 24 hours after stress. The results showed that the expression of this gene increased with increasing the duration of stress in both varieties. Comparison of the two varieties showed that the expression of <i>SBPase</i> gene in the tolerant genotype was twice as high as sensitive. Finally, <i>SBPase</i> gene as a key gene for salinity stress was cloned in both cloning (pTG19) and expression (pBI121) vectors.</p><p><strong>Conclusions: </strong>According to our results, SBPase gene increased growth and photosynthesis in barley under various abiotic stresses, therefore, over-expression of this gene in barley is recommended to produce plants resistant to abiotic stresses.</p>","PeriodicalId":14492,"journal":{"name":"Iranian Journal of Biotechnology","volume":"21 3","pages":"e3389"},"PeriodicalIF":1.3,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10858358/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139722526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SOX2 Overlapping Transcript (<i>SOX2-OT</i>) Enhances the Lung Cancer Malignancy Through Interaction with <i>miR-194-5p/</i>SOX5 Axis.","authors":"Zahra Sadeghi, Fatemeh Dodangeh, Jamshid Raheb","doi":"10.30498/ijb.2023.364919.3530","DOIUrl":"https://doi.org/10.30498/ijb.2023.364919.3530","url":null,"abstract":"<p><strong>Background: </strong>Lung cancer is one of the most common types of cancer and a leading cause of cancer-related deaths worldwide. Therefore, it is useful to know the biomarkers involved in the malignancy of lung cancer.</p><p><strong>Objectives: </strong>This study aimed to show that <i>SOX2-OT</i> as a long non-coding RNA (IncRNA) regulates gene expression via the <i>SOX2-OT/miR-194-5p/</i>SOX5 axis molecular pathway in lung cancer.</p><p><strong>Materials and methods: </strong>A549 cells transfected with siRNA-<i>SOX2-OT</i> and the expression of <i>SOX2-OT</i> and <i>miR-194-5p</i> genes were analyzed by real-time PCR before and after transfection. In addition, the expression of the B-catenin, MMP9, phosphorylated and activated STAT3 (p-STAT3), SOX5, and VEGF proteins before and after transfection was investigated by Western blotting.</p><p><strong>Results: </strong>After using siRNA-<i>SOX2-OT</i>, an increase in the expression of <i>miR-194-5p</i> and a decrease in the expression of B-catenin, SOX5, p-STAT3 activated STAT3, VEGF, and MMP9 proteins was observed.</p><p><strong>Conclusions: </strong>According to the results of the present study, an increase in <i>SOX2-OT</i> in lung cancer seems to stimulate the expression of beta-catenin, SOX5, MMP9, and VEGF thus support the malignancy of lung cancer cells.</p>","PeriodicalId":14492,"journal":{"name":"Iranian Journal of Biotechnology","volume":"21 3","pages":"e3530"},"PeriodicalIF":1.3,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10858351/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139722530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Construction of an <i>STK11</i> Mutation and Immune-Related Prognostic Prediction Model in Lung Adenocarcinoma.","authors":"Bo Tang,&nbsp;Xia Zhao,&nbsp;Hongbing Liu,&nbsp;Qingfeng Zhang,&nbsp;Kui Liu,&nbsp;Xiaoyan Yang,&nbsp;Yun Huang","doi":"10.30498/ijb.2022.307202.3168","DOIUrl":"https://doi.org/10.30498/ijb.2022.307202.3168","url":null,"abstract":"<p><strong>Background: </strong><i>STK11</i> mutation in LUAD affects immune cell infiltration in tumor tissue, and is associated with tumor prognosis.</p><p><strong>Objective: </strong>This study aimed to construct a <i>STK11</i> mutation and immune-related LUAD prognostic model.</p><p><strong>Materials and methods: </strong>The mutation frequency of <i>STK11</i> in LUAD was queried via cBioPortal in TCGA and PanCancer Atlas databases. The degree of immune infiltration was analyzed by CIBERSORT analysis. DEGs in <i>STK11</i>mut and <i>STK11</i>wt samples were analyzed. Metascape, GO and KEGG methods were adopted for functional and signaling pathway enrichment analysis of DEGs. Genes related to immune were overlapped with DEGs to acquire immune-related DEGs, whose Cox regression and LASSO analyses were employed to construct prognostic model. Univariate and multivariate Cox regression analyses verified the independence of riskscore and clinical features. A nomogram was established to predict the OS of patients. Additionally, TIMER was introduced to analyze relationship between infiltration abundance of 6 immune cells and expression of feature genes in LUAD.</p><p><strong>Results: </strong>The mutation frequency of <i>STK11</i> in LUAD was 16%, and the degrees of immune cell infiltration were different between the wild-type and mutant <i>STK11</i>. DEGs of <i>STK11</i> mutated and unmutated LUAD samples were mainly enriched in immune-related biological functions and signaling pathways. Finally, 6 feature genes were obtained, and a prognostic model was established. Riskscore was an independent immuno-related prognostic factor for LUAD. The nomogram diagram was reliable.</p><p><strong>Conclusion: </strong>Collectively, genes related to <i>STK11</i> mutation and immunity were mined from the public database, and a 6-gene prognostic prediction signature was generated.</p>","PeriodicalId":14492,"journal":{"name":"Iranian Journal of Biotechnology","volume":"21 2","pages":"e3168"},"PeriodicalIF":1.3,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/23/0b/IJB-21-e3168.PMC10203181.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9578693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Response Surface Methodology to Optimize the Expression Efficiency of Recombinant Reteplase.","authors":"Farhad Farzaneh, Sako Mirzaie, Ehsan Dehnavi, Mojtaba Aghaeepoor, Shirin Farzaneh, Navid Pourzardosht, Saeed Khalili","doi":"10.30498/ijb.2023.330285.3288","DOIUrl":"10.30498/ijb.2023.330285.3288","url":null,"abstract":"<p><strong>Background: </strong>Over expression of Reteplase enzyme has already been studies in the periplasmic space of <i>Escherichia coli</i> (<i>E. coli</i>). However, the role different factors in its expresssin rate remained to be elucidated.</p><p><strong>Objectives: </strong>Optical cell density (OD), IPTG concentration, and expression time are highly effective in the protein expression rates. Therefore, we aimed to determine the optimum levels of these factors for reteplase expression using response surface methodology (RSM).</p><p><strong>Materials and methods: </strong>The pET21b plasmid was used to sub-clone the designed reteplase gene. Then, the gene was transformed into <i>E. coli</i> BL21 strain. Induction of expression was done by IPTG and analyzed by the SDS page. experiments were designed using the RMS, while the effects of different conditions were evaluated using the Real time-PCR.</p><p><strong>Results: </strong>Sequence optimization removed all undesirable sequences of the designed gene. Transformation into <i>E. coli</i> BL21 was confirmed with an 1152 bp band on the agarose gel. A 39 kDa expression band on the SDS gel confirmed the gene expression. Performing 20 RSM-designed experiments, the optimum levels for IPTG concentration and OD were determined as 0.34mM and 5.6, respectively. Moreover, the optimum level of expression time was demonstrated to be 11.91 hours. The accuracy of the regression model for reteplase overexpression was confirmed by an F-value equal to 25.31 and a meager probability value [(Prob > F) < 0.0001]. The real-time-PCR results indicated that the performed calculations were highly accurate.</p><p><strong>Conclusion: </strong>The obtained results indicate that IPTG concentration, OD, and expression time are significantly involved in the augmentation of recombinant reteplase expression. To the best of our knowledge, this is the first study to assess the combined effect of these factors on reteplase expression. Further RSM-based experiments would bring about new insights regarding the best conditions for reteplase expression.</p>","PeriodicalId":14492,"journal":{"name":"Iranian Journal of Biotechnology","volume":"21 2","pages":"e3288"},"PeriodicalIF":1.6,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/73/33/IJB-21-e3288.PMC10203180.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9578691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-Objective Optimization of Copper Bioleaching: Comparative Study of Pure and Co-Cultured Cultivation.","authors":"Yasin Rakhshani,&nbsp;Sayyed Shahryar Rahpeyma,&nbsp;Fatemeh Tabandeh,&nbsp;Mahmood Arabnezhad,&nbsp;Ali Azimi,&nbsp;Jamshid Raheb","doi":"10.30498/ijb.2023.328969.3278","DOIUrl":"https://doi.org/10.30498/ijb.2023.328969.3278","url":null,"abstract":"<p><strong>Background: </strong>Bioleaching is a practical method to recover metals from low-grade mineral sulfides. The most frequent bacteria involved in the bioleaching of metals from ores are <i>Acidithiobacillus ferrooxidans</i> and <i>Acidithiobacillus thiooxidans</i>. Experimental design is a method through which the optimum activity condition will be obtained, avoiding numerous trials and errors.</p><p><strong>Objectives: </strong>This study aimed to optimize the bioleaching condition of two indigenous iron- and sulfur-oxidizing bacteria from the Meydouk mine, Iran, and evaluate their function in a semi-pilot operation in pure and mixed cultures.</p><p><strong>Material and methods: </strong>After treatment with sulfuric acid, the bacterial DNA was extracted, and further 16S rRNA was sequenced to characterize the bacterial species. The cultivation condition of these bacteria was optimized using Design-expert (6.1.1 version) software. The copper recovery rate and the differentiation in the ORP rate in the percolation columns were also investigated. These strains were isolated from the Meydouk mine for the first time.</p><p><strong>Results: </strong>16S rRNA analysis revealed that both bacteria belong to the <i>Acidithiobacillus</i> genus. The factors with the most significant impact on <i>Acidithiobacillus ferrooxidans</i> with their optimum level were temperature=35 °C, pH=2.5, and initial FeSO₄ concentration=25 g.L^-1. Also, initial sulfur concentration had the most significant impact on <i>Acidithiobacillus thiooxidans</i> with the optimum level of 35 g.L^-1. Moreover, the mixed culture determined higher bioleaching efficiency compared with the case of employing the pure cultures.</p><p><strong>Conclusions: </strong>Utilizing a mixture of both bacteria, <i>Acidithiobacillus ferrooxidans</i> and <i>Acidithiobacillus thiooxidans</i> elevated the Cu recovery rate due to the synergetic function of the strains. Also, introducing an initial dosage of sulfur and pre-acidification could elevate metal recovery efficiency.</p>","PeriodicalId":14492,"journal":{"name":"Iranian Journal of Biotechnology","volume":"21 2","pages":"e3278"},"PeriodicalIF":1.3,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/bb/3e/IJB-21-e3278.PMC10203187.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9880498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation and Characterization of Crayfish (<i>Astacus leptodactylus</i>) Chitosan with Different Deacetylation Degrees.","authors":"Ali Eslem Kadak,&nbsp;Aygül Küçükgülmez,&nbsp;Mehmet Çelik","doi":"10.30498/ijb.2023.323958.3253","DOIUrl":"https://doi.org/10.30498/ijb.2023.323958.3253","url":null,"abstract":"<p><strong>Background: </strong>In this study, chitosan with various deacetylation degrees was extracted from crayfish (<i>Astacus leptodactylus</i>) shells with the purpose of examining the effect of deacetylation on the characterization of chitosan.</p><p><strong>Objectives: </strong>Recycling of wastes has become an important issue with the advancement of shellfish processing technology. Therefore, this study examined the most important and conventional characterization parameters of chitosan extracted from crayfish shells and investigated whether crayfish chitosan can be an alternative to commercial products.</p><p><strong>Material and methods: </strong>In order to determine the characterization of the chitosan; degree of deacetylation, yield, molecular weight, apparent viscosity, water binding capacity, fat binding capacity, moisture content, ash content, color properties, Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), and X-ray diffraction analyses (XRD) were applied.</p><p><strong>Results: </strong>The low (LDD) and high (HDD) deacetylated crayfish chitosan characterization results in terms of yield, molecular weight, apparent viscosity, water binding capacity, fat binding capacity, moisture content, ash content were 17.50%, 424.03-334.66 kDa, 16.82-9.63 cP, 481.29-428.04%, 419.30-355.75%, 3.32-1.03%, 0.98-1.01%, respectively. As detected by two different methods, potentiometric titration and elemental analysis, the deacetylation degrees of low and high crayfish chitosan were found close to each other, which were 76.98-94.98% and 73.79-92.06%, respectively. As the deacetylation period extended, acetyl groups were removed, and the degree of the deacetylation of crayfish chitosan increased while the apparent viscosity, molecular weight, water and fat binding capacity decreased.</p><p><strong>Conclusions: </strong>The findings of the present study are important to obtain the chitosan having various physicochemical characteristics from unevaluated crayfish wastes and to use it in many different sectors, especially biotechnology, medicine, pharmaceutical, food, and agriculture.</p>","PeriodicalId":14492,"journal":{"name":"Iranian Journal of Biotechnology","volume":"21 2","pages":"e3253"},"PeriodicalIF":1.3,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/bd/7d/IJB-21-e3253.PMC10203182.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9578690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}