Iranian Journal of Biotechnology最新文献

{"title":"Impact of <i>NOX4</i> Knockout by CRISPR/Cas9 on the MCF-7, HCA-7 and UM-RC-6 Cancer Cells.","authors":"Marzieh Javadi, Hossein Sazegar, Abbas Doosti","doi":"10.30498/ijb.2022.298496.3115","DOIUrl":"https://doi.org/10.30498/ijb.2022.298496.3115","url":null,"abstract":"<p><strong>Background: </strong>The second most common cause of mortality is cancer. Increased NOX4 expression is linked to cancer development and metastasis. However, the significance of NOX4 in cell growth and assault, remains unclear.</p><p><strong>Objective: </strong>This study aimed to evaluate the effect of <i>NOX4</i> knockouts in MCF7, UM-RC-6, HCA-7 cell lines.</p><p><strong>Materials and methods: </strong>The <i>NOX4</i> gene was knocked out in MCF7, UM-RC-6, and HCA-7 cell lines through using CRISPR Cas-9 genetic engineering techniques. After transfection, the CRISPR Cas-9 cassette, the T7 endonuclease I, qPCR, and western blotting assay detected the <i>NOX4</i> knockouts. MTT and Annexin assessed the percentage of cell proliferation and apoptosis. Real-time PCR was used to measure the expression of pro- and anti-apoptotic genes.</p><p><strong>Results: </strong>Occurrence of <i>NOX4</i> gene knockout in the examined cell lines, was confirmed by q-PCR and Western blot (P<0.001). The <i>NOX4</i>-deleted cell lines with increased sub-G1 caused lowered cell proliferation and population at S / G2/ M phases. <i>In Vitro</i>, <i>NOX4</i> silencing caused lowered expressions of anti-apoptosis genes <i>BCL-2</i> and <i>SURVIVIN</i> (P<0.0001), leading to increased tendency of apoptosis in the cell lines (P<0.0001) of the apoptotic genes BAX, P53, FAS. Additionally, the MTT and Annexin results of the target gene <i>NOX4</i> knockout inhibited proliferation, increased mortality rates (P<0.01), and increased apoptosis.</p><p><strong>Conclusion: </strong>The findings of this study indicate that using <i>NOX4</i> as a target can have therapeutic value for creating potential treatments against breast, colorectal, and kidney cancers which shows a need for a deeper understanding of the biology of these cancers with direct clinical outcomes for developing novel treatment strategies.</p>","PeriodicalId":14492,"journal":{"name":"Iranian Journal of Biotechnology","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10858368/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139722608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MiR-582 Down-Regulates Lissencephaly-1 (<i>LIS1</i>) via P-Akt and MMP-2 to Inhibit Cholangiocarcinoma Cell Proliferation and Invasion.","authors":"Guochao Liu, Tao Li, Lifeng Ma, Jianlong Wang, Zhaoqiang Yin","doi":"10.30498/ijb.2022.301092.3136","DOIUrl":"https://doi.org/10.30498/ijb.2022.301092.3136","url":null,"abstract":"<p><strong>Background: </strong>Cholangiocarcinoma is a primary malignant tumor, and its progression involves oncogene activation, the absence of tumor suppressor gene, abnormal signaling pathways and miRNA expression. MiRNAs are abnormally expressed in many types of tumors.</p><p><strong>Objective: </strong>This study aims to observe the effects of miR-582 on cholangiocarcinoma cell proliferation, S-phase arrest, migration and invasion and to analyze the regulation of miR-582 on <i>LIS1</i> to clarify the real role of miR-582 in cholangiocarcinoma development.</p><p><strong>Materials and methods: </strong>TCGA database of cholangiocarcinoma samples was analyzed. Dual fluorescence reporter and TargetScan were conducted to confirm whether <i>LIS1</i> was the target gene of miR-582. Effects of miR-582 and <i>LIS1</i> on HCC-9810 cell proliferation, S-phase cell ratio, migration and invasion were determined by CCK-8, Flow cytometry and Transwell, respectively, whereas the function of miR-582 on MMP-2 and P-Akt expression was identified by Western blotting. Nude mice xenograft model of cholangiocarcinoma was established to detect what miR-582 did for tumor growth.</p><p><strong>Results: </strong>TCGA showed that miR-582 was lowly expressed and LIS1 was highly expressed in tumor tissues compared with adjacent tissues. MiR-582 targeted <i>LIS1</i> to inhibit MMP-2 and p-AKT expression. Transfection of miR-582 mimics could suppress HCC-9810 cell proliferation, S-stage arrest, migration and invasion, while <i>LIS1</i> worked oppositely. MiR-582 inhibitors promoted cell biological behavior, whereas <i>LIS1</i> siRNA was opposite. In nude mice xenograft model, miR-582 overexpression inhibited tumor growth.</p><p><strong>Conclusions: </strong>It implies that miR-582 could negatively regulate <i>LIS1</i> to inhibit MMP-2 and P-Akt expression, thus suppressing cell invasion and proliferation in cholangiocarcinoma.</p>","PeriodicalId":14492,"journal":{"name":"Iranian Journal of Biotechnology","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10858362/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139722609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis of Silver Nanoparticles by <i>Raoultella Planticola</i> and Their Potential Antibacterial Activity Against Multidrug-Resistant Isolates.","authors":"Karzan Qurbani, Safin Hussein, Haider Hamzah, Saman Sulaiman, Rzgar Pirot, Elahe Motevaseli, Zahra Azizi","doi":"10.30498/ijb.2022.298773.3121","DOIUrl":"https://doi.org/10.30498/ijb.2022.298773.3121","url":null,"abstract":"<p><strong>Background: </strong>Nanoparticles can be chemically, physically, or biologically synthesized. Biosynthesis of silver nanoparticles (AgNPs) utilizing microbes is a promising process due to the low toxicity and high stability of AgNPs. Here, AgNPs were fabricated by Gram-negative <i>Raoultella planticola</i>.</p><p><strong>Objectives: </strong>This study aimed to assess the ability of <i>Raoultella planticola</i> to produce nanoparticles (NPs) and evaluate their antibacterial potential against multidrug-resistant pathogens (MDR). Additionally, the study aimed to compare the antibacterial activity of biosynthesized nanoparticles to well-known conventional antibiotics Azithromycin and Tetracycline.</p><p><strong>Materials and methods: </strong>AgNPs were characterized using visual observation, UV-visible spectroscopy (UV-vis), X-ray diffraction (XRD), transmission electron microscopy (TEM), scanning electron microscopy (SEM), and Fourier-transform infrared spectroscopy (FTIR). The TEM and SEM were used to determine the size and shape of the nanoparticles. The XRD data were recorded in the 2θ ranging from 20-80° to analyze the crystalline structure of nanoparticles. The antibacterial activity was detected using a 96-well microtiter plate.</p><p><strong>Results: </strong>The UV-vis absorption recorded from the 300 - 900 nm spectrum was well defined at 420 nm, and the XRD pattern was compatible with Braggs's reflection of the silver nanocrystals. FTIR showed absorbance bands corresponding to different functional groups. TEM and SEM images showed non-uniform spherical and AgNPs of 10-80 nm. XRD data confirmed that the resultant particles are AgNPs. The AgNPs showed effective activity against multi-drug resistant (MDR) <i>Pseudomonas aeruginosa</i>, <i>Salmonella</i> sp., <i>Shigella</i> sp., <i>E. coli</i>, <i>Enterobacter</i> sp., <i>Staphylococcus aureus</i>, and <i>Bacillus cereus</i>. The AgNPs demonstrated effectiveness in lower concentrations compared to broad-spectrum antibiotics.</p><p><strong>Conclusion: </strong>These data reveal that AgNP generated by <i>R. planticola</i> was more efficient against MDR microorganisms than commercial antibiotics. However, the cytotoxicity of these nanoparticles must be further studied.</p>","PeriodicalId":14492,"journal":{"name":"Iranian Journal of Biotechnology","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10858361/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139722612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Co-Expression Network Analysis of Soybean Transcriptome Identify Hub Genes Under Saline-Alkaline and Water Deficit Stress.","authors":"Rahele Ghanbari Moheb Seraj, Mahsa Mohammadi, Mehrdad Shahbazi, Masoud Tohidfar","doi":"10.30498/ijb.2022.299306.3124","DOIUrl":"https://doi.org/10.30498/ijb.2022.299306.3124","url":null,"abstract":"<p><strong>Background: </strong>Soybean is an important oilseed crop that its development and production are affected by environmental stresses (such as saline-alkaline and water deficit).</p><p><strong>Objectives: </strong>This experiment was performed with the aim of identifying candidate genes in saline-alkaline stress and water-deficit stress conditions using transcriptome analysis and to investigate the expression of these genes under water deficit stress conditions using RTqPCR.</p><p><strong>Materials and methods: </strong>In this experiment, soybean transcriptome data under saline-alkaline and water-deficit stress were downloaded from the NCBI website, and then the co-expression modules were determined for them and the gene network was plotted for each module, and finally, the hub genes were identified. To compare the expression of genes in saline-alkaline and water deficit conditions, soybean plants were subjected to water deficit stress and their gene expression was determined using RTqPCR.</p><p><strong>Results: </strong>The filtered (Log FC above +2 and below -2) genes of soybean were grouped under saline-alkaline stress in 15 modules and under water-deficit stress in 2 different modules. Within each module, the interaction of genes was identified using the gene network, then three genes of <i>ann11</i>, <i>cyp450</i> and <i>zfp</i> selected as hub genes. These hub genes are highly co-expression with other network genes, which not only display differential expression but also differential co-expression. The results of RT-PCR indicated that <i>cyp450</i> gene expression was not significantly different from the control, while <i>ann11</i> gene expression significantly increased under water deficit stress, but <i>zfp</i> gene expression decreased significantly under water deficit stress.</p><p><strong>Conclusions: </strong>We identified three genes, <i>ann11</i>, <i>cyp450</i> and <i>zfp</i>, as hub genes. According to our results, <i>ann11</i> gene had a significant increase in expression under water deficit stress, which can indicate the importance of this gene under drought conditions. Therefore, according to the results of this experiment as well as other researchers, we introduce this gene as a key gene in water deficit tolerance and recommend its use in genetic engineering to increase the tolerance of other plants.</p>","PeriodicalId":14492,"journal":{"name":"Iranian Journal of Biotechnology","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10858369/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139722606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phenotype Compensation in Reproductive ADAM Gene Family: A Case Study with ADAM27 Knockout Mouse.","authors":"Jafar Khezri, Ehsan Hasheni, Mehdi Shams Ara, Aidin Rahim Tayefe, Farid Heidari","doi":"10.30498/ijb.2022.250175.2902","DOIUrl":"https://doi.org/10.30498/ijb.2022.250175.2902","url":null,"abstract":"<p><strong>Background: </strong>A disintegrin and metalloproteinase (ADAM) cell surface proteins are expressed in different cells and are involved in biological processes such as cell-cell interactions, cell differentiation, sperm attachment and fertilization. A significant number of ADAM isoforms are expressed in the reproductive tracts of male mice and other mammals, which shows the importance of this gene family in reproduction.</p><p><strong>Objectives: </strong>The role of ADAM27 protein in reproduction was investigated.</p><p><strong>Materials and methods: </strong>ADAM27 knock-out mutant mice were generated using the blastocyst microinjection technique. The knock-out mice were analyzed genetically and phenotypically to discover any abnormalities.</p><p><strong>Results: </strong>The results of this study revealed that the homozygote mutant male mice were fertile and showed no significant differences compared to wild-type male mice. A histological exam, sperm analysis and in-vitro fertilization experiments showed no statistical differences.</p><p><strong>Conclusions: </strong>We can conclude that the role of deficient ADAM27 protein is probably compensated mainly by other ADAM isoforms which are expressed in the reproductive system.</p>","PeriodicalId":14492,"journal":{"name":"Iranian Journal of Biotechnology","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10858364/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139722610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adipose-Derived Mesenchymal Stem Cells Differentiation Toward Cardiomyocyte-Like Cells on the PCL/PANI Nanofibrous Scaffold: An Experimental Study.","authors":"Parastoo Tambrchi, Morteza DaliriJoupari, Amir Hossein Mahdavi, Leila Soltani","doi":"10.30498/ijb.2022.316370.3205","DOIUrl":"https://doi.org/10.30498/ijb.2022.316370.3205","url":null,"abstract":"<p><strong>Background: </strong>Owing to the fact that the heart tissue is not able to repair itself. Biomaterial-based scaffolds are important cues in tissue engineering (TE) applications. Recent advances in TE have led to the development of suitable scaffold architecture for various tissue defects.</p><p><strong>Objective: </strong>Given the importance of cellular therapy, it was the aim of the present study to differentiate cardio myocyte cells from human adipose-derived mesenchymal stem cells (Ad-MSCs) using suitable induction reagents (namely, 5-azacytidine and transforming growth factor beta (TGF-β)) on poly-caprolactone (PCL)/Poly aniline (PANI) Nano fibrous scaffolds prepared by electrospinning.</p><p><strong>Materials and methods: </strong>For this purpose, the adipose-derived mesenchymal stem cells (Ad-MSCs) were initially isolated and characterized before cultivation on the PCL/PANI Nano fibrous scaffold to be treated for 21 days with 5-azacytidine either singly or in combination with TGF-β in medium. The scaffold's morphological and cell attachment properties were investigated using electron microscopy (SEM). Finally, the cardio myocyte differentiation of Ad-MSCs on the scaffold was studied using both quantitative Real-time PCR (qPCR) and flow-cytometry while the expression rates of the cardio myocytes' specific genes (Gata4, NKX2.5, MYH-7, and Troponin I) were also determined.</p><p><strong>Results: </strong>The results of Ad-MSCs culture, MTT assay, and SEM indicated that the cells had well proliferated on the PCL/PANI scaffolds, showing the biocompatibility of the nanofibers for cellular growth and adhesion. After 21 days of induced cardio myocyte differentiation by both agents, Real-time PCR revealed increases in the expressions of Gata4, Troponin I, MYH-7, and NKX2.5 genes in the cells cultured on the PCL/PANI scaffolds while the flow-cytometry test approved the expression of troponin I.</p><p><strong>Conclusion: </strong>The data obtained showed that the PCL/PANI Nano fibrous scaffolds were able to promote and support mesenchymal stem cell transformation to cardio myocyte cells. Generally speaking, the results of the study might be exploited in future <i>in vitro</i> and <i>in vivo</i> experimental model studies of cardio myocyte differentiation using co-polymer scaffolds.</p>","PeriodicalId":14492,"journal":{"name":"Iranian Journal of Biotechnology","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10858366/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139722605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Production of Childinan SF-2 as Bioactive Compound from <i>Daldinia childiae</i>: A Survey on Antioxidant, Antibacterial and Antitumor Properties.","authors":"Tayebeh Fooladi, Mohammad Reza Soudi, Seyed Mahmoud Hashemi, Peyman Abdeshahian","doi":"10.30498/ijb.2022.282126.3032","DOIUrl":"https://doi.org/10.30498/ijb.2022.282126.3032","url":null,"abstract":"<p><strong>Background: </strong>Fungal extracts have received increased attention due to their great medicinal applications including antitumor, immune-modulating, antioxidant, radical scavenging, antiviral, antibacterial, antifungal and detoxificating properties.</p><p><strong>Objectives: </strong>This study is the first report on a novel bioactive compound, namely Childinan SF-2 which was isolated from soil ascomycete fungus. The significant antibacterial, antioxidant and cytotoxic properties of the extract may lead to development of novel, safe and useful substances.</p><p><strong>Materials and methods: </strong>The isolate was identified on the basis of molecular approach. Spore suspension was inoculated in the culture medium and the bioactive compound was isolated from the viscous fermented broth via ethanol precipitation of the extracellular compound. The basic chemical composition of the extract including protein, carbohydrate, sulfate radical and uronic acid content were measured. FTIR (Fourier-transform infrared spectroscopy) and GC-MS (Gas chromatography-mass spectrometry) analysis were used for further structural characterization. The extract was utilized for treatment of AGS and MDA cell lines to assess the cell cycle and apoptosis. The antioxidant activity was examined using DPPH, hydroxyl radicals scavenging, β-carotene bleaching inhibition and ferric reducing power assay methods. The extract was tested for evaluation of antibacterial activity using different Gram-positive and Gram-negative bacterial strains.</p><p><strong>Results: </strong>The fungal isolate was identified as the new strain Daldinia childiae SF-2. Initial biochemical characterization of the extract showed that the fungal biopolymer was composed of total sugars, protein, uronic acids and sulfated groups with values of 91.6%, 2.15%, 2.25% and 1.05% (w/w), respectively. FTIR and GC-MS analysis revealed that Childinan SF-2 might be mainly constructed from D-glucose, D-mannitol and D-galactofuranose. The <i>in vitro</i> experiments revealed that Childinan SF-2 enhanced the percentage of necrosis and apoptosis of cancer cells and blocked the cell cycle progression as shown by flowcytometry. Childinan SF-2 represented a considerable antioxidant and antibacterial activity.</p><p><strong>Conclusions: </strong>These results indicated that Childinan SF-2 can serve as a potential source in medicinal applications. Keywords Exopolysaccharides; Fungal Biopolymers; Mycelial Secretions; Natural Pharmaceuticals; Xylariaceae.</p>","PeriodicalId":14492,"journal":{"name":"Iranian Journal of Biotechnology","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10858367/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139722611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Cross-section Metagenomics and 16S Ribosomal DNA Based Evaluation of the Bacterial and Archaeal Communities Resident in the Forumad Chromite Mine, Northeastern of Iran.","authors":"Masoumeh Sadat Mousavi Maleki, Bagher Yakhchali, Ali Asghar Karkhaneh, Mohammad Rezvani, Fatollah Ahmadpour","doi":"10.30498/ijb.2022.240607.2818","DOIUrl":"https://doi.org/10.30498/ijb.2022.240607.2818","url":null,"abstract":"<p><strong>Background: </strong>The Forumad chromite area from Sabzevar ophiolite belt, Northeastern Iran, is an environment with high concentration of heavy metals, particularly chromite and magnesite minerals, containing chromium and magnesium.</p><p><strong>Objectives: </strong>In this study for the first time, we analyzed and report the diversity of microbial (bacterial and archaeal) community inhabiting in Forumad chromite mine environment using metagenomics approach.</p><p><strong>Materials and methods: </strong>Samples were obtained from different areas of the mine, and total DNA was extracted from water and soil samples. 16S rDNA was amplified using universal primers and the PCR products were cloned in pTz57R/T plasmid. Then, 43% of the positive clones were randomly sequenced. BLAST program in NCBI and EzTaxon databases were used to identify similar 16S rDNA sequences. Phylogenetic analysis was performed using the MEGA5 software and multiple alignments of sequences.</p><p><strong>Results: </strong>In the phylogenetic analyses, <i>proteobacteria</i>, which contains many heavy metals tolerant bacteria especially chromium, were the dominant population in bacterial libraries with <i>Rheinheimera</i> and <i>Cedecaeas</i> the most abundant genuses. Other phyla were <i>Bacteroidetes</i>, <i>Firmicutes</i>, <i>Verrucomicrobia</i>, <i>Chloroflexi</i>, <i>Actinobacteria</i>, <i>Acidobacteria</i>, <i>Cyanobacteria</i>, <i>Gemmatimonadetes</i>, and <i>Planctomycetes</i>. In the archaeal clone library, all the sequences were related to the phylum <i>Thaumarchaeota</i>. Further, 68.6% of the sequences had less than 98.7℅ similarity with the recorded strains which could represent new taxons.</p><p><strong>Conclusions: </strong>The results showed that there was a high microbial diversity in the Forumad chromite area. These results can be used for detoxification and bioremediation of regions contaminated with heavy metals, although more studies are needed.</p>","PeriodicalId":14492,"journal":{"name":"Iranian Journal of Biotechnology","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10858365/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139722604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Use of Gelatin as a Sacrificial Agent in Combination with Ultrasonication to Improve Cell Infiltration and Osteogenesis of Nanofibrous PCL-nHA Scaffolds for Bone Tissue Engineering.","authors":"Saeed Moghadam Deymeh, Sameereh Hashemi-Najafabadi, Mohamadreza Baghaban-Eslaminejad, Fatemeh Bagheri","doi":"10.30498/ijb.2022.305258.3154","DOIUrl":"https://doi.org/10.30498/ijb.2022.305258.3154","url":null,"abstract":"<p><strong>Objectives: </strong>In this study, gelatin was chosen as a novel sacrificial agent in co-electrospun with polycaprolacton-nanohydroxyapatite (PCL-nHA).</p><p><strong>Materials and methods: </strong>After electrospinnig, gelatin was washed with water, and the prepared scaffold was ultrasonicated. Morphological and structural properties of the prepared scaffolds were studied by SEM. Fourier transform infrared (FTIR) spectroscopy and water contact angle analysis were used to evaluate the removal of gelatin.</p><p><strong>Results: </strong>According to the SEM results, the pore size of the modified scaffolds was increased 3-folds compared to the control sample. For PCL-nHA gelatin: (80:20) after the treatment, the average cell infiltration was 42.7 μm, while there was no infiltration for the control group. The modified electrospun scaffold significantly enhanced the osteogenic differentiation of hBMSCs as verified by increased ALP activity and upregulation of runt-related transcription factor 2 (RUNX2), collagen type 1 (COL1) and osteocalcin (OCN) genes.</p><p><strong>Conclusion: </strong>Co-electrospun PCL-nHA with gelatin as a sacrificial agent in combination with ultrasonication may be an effective, economic and controllable method to increase the pore size in electrospun scaffolds for bone tissue engineering applications.</p>","PeriodicalId":14492,"journal":{"name":"Iranian Journal of Biotechnology","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10858363/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139722613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Study on the Effect of Nitrate and Phosphate Concentrations on the Production of Mycosporine-Like Amino Acids by <i>Chlorella Vulgaris</i>.","authors":"Tahereh Hosseinabadi,&nbsp;Rouzbeh Gharib,&nbsp;Shayan Salehian,&nbsp;Maryam Tabarzad","doi":"10.30498/ijb.2022.313739.3194","DOIUrl":"https://doi.org/10.30498/ijb.2022.313739.3194","url":null,"abstract":"<p><strong>Background: </strong>Cyanobacteria can produce compounds absorbing ultraviolet irradiation. Mycosporine like amino acids (MAAs) are some of these important metabolites, which can be potentially considered as a sunscreen agent in the pharmaceutical and cosmetic industry. Different factors have been reported that can affect the biosynthesis of MAA.</p><p><strong>Objective: </strong>In this study, the influence of different concentrations of phosphate and nitrate under different environmental conditions on MAA production by <i>Chlorella vulgaris</i> was investigated using an experimental design method, in order to enhance MAAs production in this specious.</p><p><strong>Materials and methods: </strong>A 2³ full factorial design (FFD) using Design-Expert v7.0.0 software was used to optimize simultaneously all the three factors of nitrate and phosphate concentration and condition of incubation environment on the MAA production by this species of <i>C. vulgaris</i>. Two milliliter of organism stock were grown in 200 mL BG11 medium and after 21 days, the biomasses of all samples were separated. Then, the MAA was extracted from dried biomass using methanol extraction. The extracts were analyzed by reverse-phase high performance liquid chromatography (RP-HPLC). After complete analysis, four samples were then cultured at the optimized conditions and analyzed by liquid chromatohraphy coupled to mass spectrometry (LC/MS).</p><p><strong>Results: </strong>The results showed that this microalga could produce compounds with λ^max of 330 nm and a retention time of about 2 min. According to the central composite analysis, phosphate at 0.51 g.L^-1 and nitrate at 2.5 g.L^-1 can be considered as the optimum concentrations, resulting to the preferable conditions concerning the culture in germinator. Based on LC/MSS analysis, the major compound had a m/z of 332 at the optimum condition.</p><p><strong>Conclusion: </strong>Thus, this species is expected to have the capability of MAA production (maybe Shinorine) or one of its glycosylated derivatives.</p>","PeriodicalId":14492,"journal":{"name":"Iranian Journal of Biotechnology","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/f6/f8/IJB-20-e3194.PMC9618020.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40704276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}