利用蓝藻提高薄荷精油的生产和一些薄荷醇生物合成相关基因的表达。

IF 1.6 4区 生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Ali Akbar Ghotbi-Ravandi, Zeinab Shariatmadari, Hossein Riahi, Seyedeh Batool Hassani, Fatemeh Heidari, Majid Ghorbani Nohooji
{"title":"利用蓝藻提高薄荷精油的生产和一些薄荷醇生物合成相关基因的表达。","authors":"Ali Akbar Ghotbi-Ravandi, Zeinab Shariatmadari, Hossein Riahi, Seyedeh Batool Hassani, Fatemeh Heidari, Majid Ghorbani Nohooji","doi":"10.30498/ijb.2023.368377.3550","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong><i>Mentha piperita</i> L. is one of the most important aromatic crops and is cultivated worldwide for essential oils (EOs).</p><p><strong>Objectives: </strong>The aim of the present study was to investigate the potential of two cyanobacteria, <i>Anabaena vaginicola</i> ISB42 and <i>Nostoc spongiaeforme</i> var. <i>tenue</i> ISB65, as biological-elicitors to improve the growth and essential oil production of <i>M. piperita</i>.</p><p><strong>Materials and methods: </strong>In this experiment, inoculation of <i>M. piperita</i> with cyanobacteria was performed by adding 1% cyanobacterial suspension to the soil of treated pots on the first time of planting and every 20 days thereafter. The experiment was performed in a randomized complete block design in an experimental greenhouse condition. After 90 days planting, the vegetative growth factors, the content of photosynthetic pigments, as well as the quantity and quality of EOs of treated and control plants were evaluated. Also, quantitative changes in the expression of some menthol biosynthesis-related genes were investigated.</p><p><strong>Results: </strong>Cyanobacterial application led to significant increases in <i>M. piperita</i> growth indices including root and shoot biomass, leaf number, leaf area, node number and ramification, as well as photosynthetic pigments content. The statistical analysis showed a 41-75 % increase in some of these growth indices, especially in Nostoc-treated plants. <i>A. vaginicola</i> and <i>N. spongiaeforme</i> var. <i>tenue</i> inoculation led to a 13% and 25% increase in the EOs content of <i>M. piperita</i>, respectively. The EOs components were also affected by cyanobacterial treatments. According to the statistical analysis, Nostoc-treated plants showed the highest amount of (-)-menthone and (-)-limonene, with a 2.36 and 1.87-fold increase compared to the control. <i>A. vaginicola</i> and <i>N. spongiaeforme</i> var. <i>tenue</i> inoculation also led to 40% and 98% increase in transcript level of (-)-limonene synthase gene, respectively. The expression of the (-)-menthone reductase gene, was also increased by 65% and 55% in response to <i>A. vaginicola</i> and <i>N. spongiaeforme</i> var. <i>tenue</i> application, respectively.</p><p><strong>Conclusions: </strong>Our data demonstrated that in addition to growth enhancement, these two heterocystous cyanobacteria improved the quantity and quality of EOs by up-regulating the key genes involved in the menthol biosynthetic pathway. Based on our results, these cyanobacteria can be considered valuable candidates in the formulation of low-cost and environmentally friendly biofertilizers in sustainable peppermint production.</p>","PeriodicalId":14492,"journal":{"name":"Iranian Journal of Biotechnology","volume":null,"pages":null},"PeriodicalIF":1.6000,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10804067/pdf/","citationCount":"0","resultStr":"{\"title\":\"Enhancement of Essential Oil Production and Expression of Some Menthol Biosynthesis-Related Genes in <i>Mentha piperita</i> Using Cyanobacteria.\",\"authors\":\"Ali Akbar Ghotbi-Ravandi, Zeinab Shariatmadari, Hossein Riahi, Seyedeh Batool Hassani, Fatemeh Heidari, Majid Ghorbani Nohooji\",\"doi\":\"10.30498/ijb.2023.368377.3550\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong><i>Mentha piperita</i> L. is one of the most important aromatic crops and is cultivated worldwide for essential oils (EOs).</p><p><strong>Objectives: </strong>The aim of the present study was to investigate the potential of two cyanobacteria, <i>Anabaena vaginicola</i> ISB42 and <i>Nostoc spongiaeforme</i> var. <i>tenue</i> ISB65, as biological-elicitors to improve the growth and essential oil production of <i>M. piperita</i>.</p><p><strong>Materials and methods: </strong>In this experiment, inoculation of <i>M. piperita</i> with cyanobacteria was performed by adding 1% cyanobacterial suspension to the soil of treated pots on the first time of planting and every 20 days thereafter. The experiment was performed in a randomized complete block design in an experimental greenhouse condition. After 90 days planting, the vegetative growth factors, the content of photosynthetic pigments, as well as the quantity and quality of EOs of treated and control plants were evaluated. Also, quantitative changes in the expression of some menthol biosynthesis-related genes were investigated.</p><p><strong>Results: </strong>Cyanobacterial application led to significant increases in <i>M. piperita</i> growth indices including root and shoot biomass, leaf number, leaf area, node number and ramification, as well as photosynthetic pigments content. The statistical analysis showed a 41-75 % increase in some of these growth indices, especially in Nostoc-treated plants. <i>A. vaginicola</i> and <i>N. spongiaeforme</i> var. <i>tenue</i> inoculation led to a 13% and 25% increase in the EOs content of <i>M. piperita</i>, respectively. The EOs components were also affected by cyanobacterial treatments. According to the statistical analysis, Nostoc-treated plants showed the highest amount of (-)-menthone and (-)-limonene, with a 2.36 and 1.87-fold increase compared to the control. <i>A. vaginicola</i> and <i>N. spongiaeforme</i> var. <i>tenue</i> inoculation also led to 40% and 98% increase in transcript level of (-)-limonene synthase gene, respectively. The expression of the (-)-menthone reductase gene, was also increased by 65% and 55% in response to <i>A. vaginicola</i> and <i>N. spongiaeforme</i> var. <i>tenue</i> application, respectively.</p><p><strong>Conclusions: </strong>Our data demonstrated that in addition to growth enhancement, these two heterocystous cyanobacteria improved the quantity and quality of EOs by up-regulating the key genes involved in the menthol biosynthetic pathway. Based on our results, these cyanobacteria can be considered valuable candidates in the formulation of low-cost and environmentally friendly biofertilizers in sustainable peppermint production.</p>\",\"PeriodicalId\":14492,\"journal\":{\"name\":\"Iranian Journal of Biotechnology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.6000,\"publicationDate\":\"2023-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10804067/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Iranian Journal of Biotechnology\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.30498/ijb.2023.368377.3550\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Iranian Journal of Biotechnology","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.30498/ijb.2023.368377.3550","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

背景:薄荷(Mentha piperita L.)是最重要的芳香作物之一,全世界都在种植薄荷精油(EOs):本研究旨在探讨两种蓝藻 Anabaena vaginicola ISB42 和 Nostoc spongiaeforme var:在本实验中,通过在第一次种植时向处理过的花盆土壤中添加 1%的蓝藻悬浮液来对蒌蒿进行蓝藻接种,之后每 20 天接种一次。实验采用随机完全区组设计,在实验温室条件下进行。种植 90 天后,对处理过的植物和对照植物的植株生长素、光合色素含量以及环氧乙烷的数量和质量进行了评估。此外,还研究了一些薄荷醇生物合成相关基因表达的定量变化:结果:施用蓝藻能显著提高蒌蒿的生长指数,包括根和芽的生物量、叶片数、叶面积、节数和分枝率,以及光合色素含量。统计分析显示,其中一些生长指数增加了 41-75%,特别是在 Nostoc 处理过的植物上。接种 A. vaginicola 和 N. spongiaeforme var. tenue 后,M. piperita 的环氧乙烷含量分别增加了 13% 和 25%。EOs 成分也受到蓝藻处理的影响。根据统计分析,经 Nostoc 处理的植物显示出最高的 (-)-menthone 和 (-)-limonene 含量,与对照相比分别增加了 2.36 倍和 1.87 倍。接种 A. vaginicola 和 N. spongiaeforme var. tenue 也分别导致(-)-柠檬烯合成酶基因转录水平增加 40% 和 98%。在接种 A. vaginicola 和 N. spongiaeforme var. tenue 后,(-)-薄荷酮还原酶基因的表达量也分别增加了 65% 和 55%:我们的数据表明,这两种杂囊蓝藻除了能促进生长外,还能通过上调参与薄荷醇生物合成途径的关键基因来提高环氧乙烷的数量和质量。根据我们的研究结果,这些蓝藻可被视为在可持续薄荷生产中配制低成本、环境友好型生物肥料的宝贵候选菌种。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Enhancement of Essential Oil Production and Expression of Some Menthol Biosynthesis-Related Genes in Mentha piperita Using Cyanobacteria.

Background: Mentha piperita L. is one of the most important aromatic crops and is cultivated worldwide for essential oils (EOs).

Objectives: The aim of the present study was to investigate the potential of two cyanobacteria, Anabaena vaginicola ISB42 and Nostoc spongiaeforme var. tenue ISB65, as biological-elicitors to improve the growth and essential oil production of M. piperita.

Materials and methods: In this experiment, inoculation of M. piperita with cyanobacteria was performed by adding 1% cyanobacterial suspension to the soil of treated pots on the first time of planting and every 20 days thereafter. The experiment was performed in a randomized complete block design in an experimental greenhouse condition. After 90 days planting, the vegetative growth factors, the content of photosynthetic pigments, as well as the quantity and quality of EOs of treated and control plants were evaluated. Also, quantitative changes in the expression of some menthol biosynthesis-related genes were investigated.

Results: Cyanobacterial application led to significant increases in M. piperita growth indices including root and shoot biomass, leaf number, leaf area, node number and ramification, as well as photosynthetic pigments content. The statistical analysis showed a 41-75 % increase in some of these growth indices, especially in Nostoc-treated plants. A. vaginicola and N. spongiaeforme var. tenue inoculation led to a 13% and 25% increase in the EOs content of M. piperita, respectively. The EOs components were also affected by cyanobacterial treatments. According to the statistical analysis, Nostoc-treated plants showed the highest amount of (-)-menthone and (-)-limonene, with a 2.36 and 1.87-fold increase compared to the control. A. vaginicola and N. spongiaeforme var. tenue inoculation also led to 40% and 98% increase in transcript level of (-)-limonene synthase gene, respectively. The expression of the (-)-menthone reductase gene, was also increased by 65% and 55% in response to A. vaginicola and N. spongiaeforme var. tenue application, respectively.

Conclusions: Our data demonstrated that in addition to growth enhancement, these two heterocystous cyanobacteria improved the quantity and quality of EOs by up-regulating the key genes involved in the menthol biosynthetic pathway. Based on our results, these cyanobacteria can be considered valuable candidates in the formulation of low-cost and environmentally friendly biofertilizers in sustainable peppermint production.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Iranian Journal of Biotechnology
Iranian Journal of Biotechnology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-
CiteScore
2.60
自引率
7.70%
发文量
20
期刊介绍: Iranian Journal of Biotechnology (IJB) is published quarterly by the National Institute of Genetic Engineering and Biotechnology. IJB publishes original scientific research papers in the broad area of Biotechnology such as, Agriculture, Animal and Marine Sciences, Basic Sciences, Bioinformatics, Biosafety and Bioethics, Environment, Industry and Mining and Medical Sciences.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信