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International journal of stem cells最新文献

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Glutathione Dynamics in the Tumor Microenvironment: A Potential Target of Cancer Stem Cells and T Cells. 肿瘤微环境中的谷胱甘肽动态：癌症干细胞和 T 细胞的潜在靶点。
IF 2.5 4区 医学
International journal of stem cells Pub Date : 2024-08-30 Epub Date: 2024-06-26 DOI: 10.15283/ijsc24060
Youngjun Park, Eui Man Jeong
{"title":"Glutathione Dynamics in the Tumor Microenvironment: A Potential Target of Cancer Stem Cells and T Cells.","authors":"Youngjun Park, Eui Man Jeong","doi":"10.15283/ijsc24060","DOIUrl":"10.15283/ijsc24060","url":null,"abstract":"<p><p>Glutathione (GSH), the main cellular antioxidant, dynamically influences tumor growth, metastasis, and resistance to therapy in the tumor microenvironment (TME), which comprises cancer cells, immune cells, stromal cells, and non-cellular components, including the extracellular matrix, metabolites, hypoxia, and acidity. Cancer stem cells (CSCs) and T cells are minor but significant cell subsets of the TME. GSH dynamics influences the fate of CSCs and T cells. Here, we explored GSH dynamics in CSCs and T cells within the TME, as well as therapeutic approaches that could target these dynamics.</p>","PeriodicalId":14392,"journal":{"name":"International journal of stem cells","volume":" ","pages":"270-283"},"PeriodicalIF":2.5,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11361844/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141450490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Establishing Three-Dimensional Explant Culture of Human Dental Pulp Tissue. 建立人类牙髓组织的三维外植体培养。
IF 2.5 4区 医学
International journal of stem cells Pub Date : 2024-08-30 Epub Date: 2024-07-12 DOI: 10.15283/ijsc23105
Eun Jin Seo, Soyoung Park, Eungyung Lee, Yang Hoon Huh, Ye Eun Ha, Gabor J Tigyi, Taesung Jeong, Il Ho Jang, Jonghyun Shin
{"title":"Establishing Three-Dimensional Explant Culture of Human Dental Pulp Tissue.","authors":"Eun Jin Seo, Soyoung Park, Eungyung Lee, Yang Hoon Huh, Ye Eun Ha, Gabor J Tigyi, Taesung Jeong, Il Ho Jang, Jonghyun Shin","doi":"10.15283/ijsc23105","DOIUrl":"10.15283/ijsc23105","url":null,"abstract":"<p><p>Mesenchymal stem cells in the dental tissue indicate a disposition for differentiation into diverse dental lineages and contain enormous potential as the important means for regenerative medicine in dentistry. Among various dental tissues, the dental pulp contains stem cells, progenitor cells and odontoblasts for maintaining dentin homeostasis. The conventional culture of stem cells holds a limit as the living tissue constitutes the three-dimensional (3D) structure. Recent development in the organoid cultures have successfully recapitulated 3D structure and advanced to the assembling of different types. In the current study, the protocol for 3D explant culture of the human dental pulp tissue has been established by adopting the organoid culture. After isolating dental pulp from human tooth, the intact tissue was placed between two layers for Matrigel with addition of the culture medium. The reticular outgrowth of pre-odontoblast layer continued for a month and the random accumulation of dentin was observed near the end. Electron microscopy showed the cellular organization and <i>in situ</i> development of dentin, and immunohistochemistry exhibited the expression of odontoblast and stem cell markers in the outgrowth area. Three-dimensional explant culture of human dental pulp will provide a novel platform for understanding stem cell biology inside the tooth and developing the regenerative medicine.</p>","PeriodicalId":14392,"journal":{"name":"International journal of stem cells","volume":" ","pages":"330-336"},"PeriodicalIF":2.5,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11361853/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141590332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cytoplasmatic Localization of Six1 in Male Testis and Spermatogonial Stem Cells. 男性睾丸和精原干细胞中 Six1 的细胞质定位
IF 2.5 4区 医学
International journal of stem cells Pub Date : 2024-08-30 Epub Date: 2024-01-16 DOI: 10.15283/ijsc23093
Mingming Qin, Linzi Ma, Wenjing Du, Dingyao Chen, Guoqun Luo, Zhaoting Liu
{"title":"Cytoplasmatic Localization of Six1 in Male Testis and Spermatogonial Stem Cells.","authors":"Mingming Qin, Linzi Ma, Wenjing Du, Dingyao Chen, Guoqun Luo, Zhaoting Liu","doi":"10.15283/ijsc23093","DOIUrl":"10.15283/ijsc23093","url":null,"abstract":"<p><p>Sine oculis homeobox 1 (Six1) is an important factor for embryonic development and carcinoma malignancy. However, the localization of Six1 varies due to protein size and cell types in different organs. In this study, we focus on the expression and localization of Six1 in male reproductive organ via bioinformatics analysis and immunofluorescent detection. The potential interacted proteins with Six1 were also predicted by protein-protein interactions (PPIs) and Enrichr analysis. Bioinformatic data from The Cancer Genome Atlas and Genotype-Tissue Expression project databases showed that <i>SIX1</i> was highly expressed in normal human testis, but low expressed in the testicular germ cell tumor sample. Human Protein Atlas examination verified that SIX1 level was higher in normal than that in cancer samples. The sub-localization of SIX1 in different reproductive tissues varies but specifically in the cytoplasm and membrane in testicular cells. In mouse cells, single cell RNA-sequencing data analysis indicated that <i>Six1</i> expression level was higher in mouse spermatogonial stem cells (mSSCs) and differentiating spermatogonial than in other somatic cells. Immunofluorescence staining showed the cytoplasmic localization of Six1 in mouse testis and mSSCs. Further PPIs and Enrichr examination showed the potential interaction of Six1 with bone morphogenetic protein 4 (Bmp4) and catenin Beta-1 (CtnnB1) and stem cell signal pathways. Cytoplasmic localization of Six1 in male testis and mSSCs was probably associated with stem cell related proteins Bmp4 and CtnnB1 for stem cell development.</p>","PeriodicalId":14392,"journal":{"name":"International journal of stem cells","volume":" ","pages":"298-308"},"PeriodicalIF":2.5,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11361848/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139471942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Human Endometrium Derived Mesenchymal Stem Cells with Aberrant NOD1 Expression Are Associated with Ectopic Endometrial Lesion Formation. NOD1表达异常的人类子宫内膜衍生间充质干细胞与异位子宫内膜病变的形成有关。
IF 2.5 4区 医学
International journal of stem cells Pub Date : 2024-08-30 Epub Date: 2024-03-27 DOI: 10.15283/ijsc22200
Chunmei Li, Suiyu Luo, Ai Guo, Ying Su, Yuhui Zhang, Yan Song, Mei Liu, Lu Wang, Yuanyuan Zhang
{"title":"Human Endometrium Derived Mesenchymal Stem Cells with Aberrant NOD1 Expression Are Associated with Ectopic Endometrial Lesion Formation.","authors":"Chunmei Li, Suiyu Luo, Ai Guo, Ying Su, Yuhui Zhang, Yan Song, Mei Liu, Lu Wang, Yuanyuan Zhang","doi":"10.15283/ijsc22200","DOIUrl":"10.15283/ijsc22200","url":null,"abstract":"<p><p>Nucleotide-binding oligomerization domain 1 (NOD1), a cytosolic pattern recognition receptor protein, plays a crucial role in innate immune responses. However, the functional expression of NOD1 in mesenchymal stem cells (MSCs) derived from endometriosis remains unclear. The aim of this study was to explore the functions of NOD1 in ectopic endometrial lesions. Tissues and MSCs were isolated from both normal endometrium and endometriosis. Immunohistochemistry and real time quantitative polymerase chain reaction (RT-qPCR) were used to determine the expression of NOD1 in the tissues/MSCs. Quantification of various cytokines was performed using RT-qPCR and enzyme-linked immunosorbent assay. To confirm the proliferation, invasion/migration, and apoptotic viabilities of the samples, Cell Counting Kit-8, clonogenic formation, transwell assays, and apoptotic experiments were conducted. Higher levels of NOD1 expression were detected in the ectopic-MSCs obtained from endometriosis compared to those from the endometrium. The expression of interleukin-8 was higher in the ectopic-MSCs than in the eutopic-MSCs. Pretreatment with NOD1 agonist significantly enhanced the proliferation and invasion/migration of eutopic-MSCs. Additionally, the NOD1 inhibitor ML-130 significantly reduced the proliferation, clone formation, invasion, and migration abilities of the ectopic-MSCs, having no effect on their apoptosis capacity. Our findings suggest that the expression of NOD1 in ectopic-MSCs may contribute to the progression of ectopic endometrial lesions.</p>","PeriodicalId":14392,"journal":{"name":"International journal of stem cells","volume":" ","pages":"309-318"},"PeriodicalIF":2.5,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11361846/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140293503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Navigating the Landscape of Intestinal Regeneration: A Spotlight on Quiescence Regulation and Fetal Reprogramming. 探索肠道再生的全貌：静止调节和胎儿重编程聚焦。
IF 2.5 4区 医学
International journal of stem cells Pub Date : 2024-08-30 Epub Date: 2024-01-25 DOI: 10.15283/ijsc23176
Su-Jeong Oh, Yoojin Seo, Hyung-Sik Kim
{"title":"Navigating the Landscape of Intestinal Regeneration: A Spotlight on Quiescence Regulation and Fetal Reprogramming.","authors":"Su-Jeong Oh, Yoojin Seo, Hyung-Sik Kim","doi":"10.15283/ijsc23176","DOIUrl":"10.15283/ijsc23176","url":null,"abstract":"<p><p>Tissue-specific adult stem cells are pivotal in maintaining tissue homeostasis, especially in the rapidly renewing intestinal epithelium. At the heart of this process are leucine-rich repeat-containing G protein-coupled receptor 5-expressing crypt base columnar cells (CBCs) that differentiate into various intestinal epithelial cells. However, while these CBCs are vital for tissue turnover, they are vulnerable to cytotoxic agents. Recent advances indicate that alternative stem cell sources drive the epithelial regeneration post-injury. Techniques like lineage tracing and single-cell RNA sequencing, combined with <i>in vitro</i> organoid systems, highlight the remarkable cellular adaptability of the intestinal epithelium during repair. These regenerative responses are mediated by the reactivation of conserved stem cells, predominantly quiescent stem cells and revival stem cells. With focus on these cells, this review unpacks underlying mechanisms governing intestinal regeneration and explores their potential clinical applications.</p>","PeriodicalId":14392,"journal":{"name":"International journal of stem cells","volume":" ","pages":"213-223"},"PeriodicalIF":2.5,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11361849/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139544729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Human Pluripotent Stem Cell-Derived Retinal Organoids: A Viable Platform for Investigating the Efficacy of Adeno-Associated Virus Gene Therapy. 人类多能干细胞衍生的视网膜器官组织：研究腺相关病毒基因疗法疗效的可行平台。
IF 2.3 4区 医学
International journal of stem cells Pub Date : 2024-05-30 Epub Date: 2024-01-22 DOI: 10.15283/ijsc23071
Hyeon-Jin Na, Jae-Eun Kwon, Seung-Hyun Kim, Jiwon Ahn, Ok-Seon Kwon, Kyung-Sook Chung
{"title":"Human Pluripotent Stem Cell-Derived Retinal Organoids: A Viable Platform for Investigating the Efficacy of Adeno-Associated Virus Gene Therapy.","authors":"Hyeon-Jin Na, Jae-Eun Kwon, Seung-Hyun Kim, Jiwon Ahn, Ok-Seon Kwon, Kyung-Sook Chung","doi":"10.15283/ijsc23071","DOIUrl":"10.15283/ijsc23071","url":null,"abstract":"<p><p>With recent advances in adeno-associated virus (AAV)-based gene therapy, efficacy and toxicity screening have become essential for developing gene therapeutic drugs for retinal diseases. Retinal organoids from human pluripotent stem cells (hPSCs) offer a more accessible and reproducible human test platform for evaluating AAV-based gene therapy. In this study, hPSCs were differentiated into retinal organoids composed of various types of retinal cells. The transduction efficiencies of AAV2 and AAV8, which are widely used in clinical trials of inherited retinal diseases, were analyzed using retinal organoids. These results suggest that retinal organoids derived from hPSCs serve as suitable screening platforms owing to their diverse retinal cell types and similarity to the human retina. In summary, we propose an optimal stepwise protocol that includes the generation of retinal organoids and analysis of AAV transduction efficacy, providing a comprehensive approach for evaluating AAV-based gene therapy for retinal diseases.</p>","PeriodicalId":14392,"journal":{"name":"International journal of stem cells","volume":" ","pages":"204-211"},"PeriodicalIF":2.3,"publicationDate":"2024-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11170113/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139512545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Essential Guidelines for Manufacturing and Application of Organoids. 有机体制造和应用基本指南》。
IF 2.3 4区 医学
International journal of stem cells Pub Date : 2024-05-30 Epub Date: 2024-05-20 DOI: 10.15283/ijsc24047
Sun-Ju Ahn, Sungin Lee, Dayeon Kwon, Sejeong Oh, Chihye Park, Sooyeon Jeon, Jin Hee Lee, Tae Sung Kim, Il Ung Oh
{"title":"Essential Guidelines for Manufacturing and Application of Organoids.","authors":"Sun-Ju Ahn, Sungin Lee, Dayeon Kwon, Sejeong Oh, Chihye Park, Sooyeon Jeon, Jin Hee Lee, Tae Sung Kim, Il Ung Oh","doi":"10.15283/ijsc24047","DOIUrl":"10.15283/ijsc24047","url":null,"abstract":"<p><p>An organoid is a self-organized three-dimensional structure derived from stem cells that mimics the structure, cell composition, and functional characteristics of specific organs and tissues and is used for evaluating the safety and effectiveness of drugs and the toxicity of industrial chemicals. Organoid technology is a new methodology that could replace testing on animals testing and accelerate development of precision and regenerative medicine. However, large variations in production can occur between laboratories with low reproducibility of the production process and no internationally agreed standards for quality evaluation factors at endpoints. To overcome these barriers that hinder the regulatory acceptance and commercialization of organoids, Korea established the Organoid Standards Initiative in September 2023 with various stakeholders, including industry, academia, regulatory agencies, and standard development experts, through public and private partnerships. This developed general guidelines for organoid manufacturing and quality evaluation and for quality evaluation guidelines for organoid-specific manufacturing for the liver, intestines, and heart through extensive evidence analysis and consensus among experts. This report is based on the common standard guideline v1.0, which is a general organoid manufacturing and quality evaluation to promote the practical use of organoids. This guideline does not focus on specific organoids or specific contexts of use but provides guidance to organoid makers and users on materials, procedures, and essential quality assessment methods at end points that are essential for organoid production applicable at the current technology level.</p>","PeriodicalId":14392,"journal":{"name":"International journal of stem cells","volume":" ","pages":"102-112"},"PeriodicalIF":2.3,"publicationDate":"2024-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11170116/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141065258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Guidelines for Manufacturing and Application of Organoids: Kidney. 有机体制造和应用指南》：肾脏。
IF 2.3 4区 医学
International journal of stem cells Pub Date : 2024-05-30 Epub Date: 2024-05-20 DOI: 10.15283/ijsc24040
Hyun Mi Kang, Dong Sung Kim, Yong Kyun Kim, Kunyoo Shin, Sun-Ju Ahn, Cho-Rok Jung
{"title":"Guidelines for Manufacturing and Application of Organoids: Kidney.","authors":"Hyun Mi Kang, Dong Sung Kim, Yong Kyun Kim, Kunyoo Shin, Sun-Ju Ahn, Cho-Rok Jung","doi":"10.15283/ijsc24040","DOIUrl":"10.15283/ijsc24040","url":null,"abstract":"<p><p>Recent advancements in organoid technology have led to a vigorous movement towards utilizing it as a substitute for animal experiments. Organoid technology offers versatile applications, particularly in toxicity testing of pharmaceuticals or chemical substances. However, for the practical use in toxicity testing, minimal guidance is required to ensure reliability and relevance. This paper aims to provide minimal guidelines for practical uses of kidney organoids derived from human pluripotent stem cells as a toxicity evaluation model <i>in vitro</i>.</p>","PeriodicalId":14392,"journal":{"name":"International journal of stem cells","volume":" ","pages":"141-146"},"PeriodicalIF":2.3,"publicationDate":"2024-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11170122/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141065261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Guidelines for Manufacturing and Application of Organoids: Skin. 有机体制造和应用指南：皮肤。
IF 2.3 4区 医学
International journal of stem cells Pub Date : 2024-05-30 Epub Date: 2024-05-24 DOI: 10.15283/ijsc24045
Seunghee Lee, Yeri Alice Rim, Juryun Kim, Su Hyon Lee, Hye Jung Park, Hyounwoo Kim, Sun-Ju Ahn, Ji Hyeon Ju
{"title":"Guidelines for Manufacturing and Application of Organoids: Skin.","authors":"Seunghee Lee, Yeri Alice Rim, Juryun Kim, Su Hyon Lee, Hye Jung Park, Hyounwoo Kim, Sun-Ju Ahn, Ji Hyeon Ju","doi":"10.15283/ijsc24045","DOIUrl":"10.15283/ijsc24045","url":null,"abstract":"<p><p>To address the limitations of animal testing, scientific research is increasingly focused on developing alternative testing methods. These alternative tests utilize cells or tissues derived from animals or humans for <i>in vitro</i> testing, as well as artificial tissues and organoids. In western countries, animal testing for cosmetics has been banned, leading to the adoption of artificial skin for toxicity evaluation, such as skin corrosion and irritation assessments. Standard guidelines for skin organoid technology becomes necessary to ensure consistent data and evaluation in replacing animal testing with <i>in vitro</i> methods. These guidelines encompass aspects such as cell sourcing, culture techniques, quality requirements and assessment, storage and preservation, and organoid-based assays.</p>","PeriodicalId":14392,"journal":{"name":"International journal of stem cells","volume":" ","pages":"182-193"},"PeriodicalIF":2.3,"publicationDate":"2024-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11170114/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141087452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Guidelines for Manufacturing and Application of Organoids: Lung. 有机体制造和应用指南》：肺
IF 2.3 4区 医学
International journal of stem cells Pub Date : 2024-05-30 Epub Date: 2024-05-23 DOI: 10.15283/ijsc24041
Kyungtae Lim, Mi-Ok Lee, Jinwook Choi, Jung-Hyun Kim, Eun-Mi Kim, Chang Gyu Woo, Chaeuk Chung, Yong-Hee Cho, Seok-Ho Hong, Young-Jae Cho, Sun-Ju Ahn
{"title":"Guidelines for Manufacturing and Application of Organoids: Lung.","authors":"Kyungtae Lim, Mi-Ok Lee, Jinwook Choi, Jung-Hyun Kim, Eun-Mi Kim, Chang Gyu Woo, Chaeuk Chung, Yong-Hee Cho, Seok-Ho Hong, Young-Jae Cho, Sun-Ju Ahn","doi":"10.15283/ijsc24041","DOIUrl":"10.15283/ijsc24041","url":null,"abstract":"<p><p>The objective of standard guideline for utilization of human lung organoids is to provide the basic guidelines required for the manufacture, culture, and quality control of the lung organoids for use in non-clinical efficacy and inhalation toxicity assessments of the respiratory system. As a first step towards the utilization of human lung organoids, the current guideline provides basic, minimal standards that can promote development of alternative testing methods, and can be referenced not only for research, clinical, or commercial uses, but also by experts and researchers at regulatory institutions when assessing safety and efficacy.</p>","PeriodicalId":14392,"journal":{"name":"International journal of stem cells","volume":" ","pages":"147-157"},"PeriodicalIF":2.3,"publicationDate":"2024-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11170115/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141081564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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