International journal of stem cells最新文献

Proteomic Profiling of Exosomes Derived from Endometrial Stem Cells and Adipose-Derived Stem Cells. 子宫内膜干细胞和脂肪干细胞外泌体的蛋白质组学分析。

IF 2.5 4区医学

International journal of stem cells Pub Date : 2025-06-18 DOI: 10.15283/ijsc25031

Jungwon Park, Jeongmin Lee, Yeon-Suk Kim, Yohan Oh

{"title":"Proteomic Profiling of Exosomes Derived from Endometrial Stem Cells and Adipose-Derived Stem Cells.","authors":"Jungwon Park, Jeongmin Lee, Yeon-Suk Kim, Yohan Oh","doi":"10.15283/ijsc25031","DOIUrl":"https://doi.org/10.15283/ijsc25031","url":null,"abstract":"Endometrial stem cells (EnSCs) are mesenchymal stem cells (MSCs) derived from endometrial tissue and serve as a valuable MSC source, as they are naturally replenished during menstruation. Exosomes, vesicles secreted by cells, contain various biomolecules such as proteins and nucleic acids and play crucial roles in intracellular communication, protein and nucleic acid metabolism, immune response regulation, and antigen presentation. This study investigated the protein profiles of EnSC-derived exosomes isolated from the endometrium of menstruating women and compared them with those of adipose-derived stem cell (ASC)-derived exosomes. After isolating EnSCs and ASCs, MSC characteristics were confirmed, and the purified exosomes were analyzed to determine their individual protein compositions. EnSCs, which can be obtained through non-invasive methods, exhibit multipotency similar to other MSCs and demonstrate rapid proliferation in vitro. Proteomic analysis of exosomal proteins revealed that 236 proteins were significantly more abundant in EnSC-derived exosomes than in ASC-derived exosomes, whereas 84 proteins were significantly more abundant in ASC-derived exosomes than in EnSC-derived exosomes. These findings indicate that EnSC-derived exosomes contain unique proteins compared to ASC-derived exosomes, as demonstrated through proteomic profiling. While further clinical studies are required, EnSCs hold promise as a potential therapeutic option in regenerative medicine, similar to current cell therapy products under development.","PeriodicalId":14392,"journal":{"name":"International journal of stem cells","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144316920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulatory Landscapes of Muscle Satellite Cells: From Mechanism to Application. 肌肉卫星细胞的调控景观：从机制到应用。

IF 2.5 4区医学

International journal of stem cells Pub Date : 2025-06-12 DOI: 10.15283/ijsc25037

Jeong Eun Lee, Sang Hoon Yoon, Kwan Seob Shim, Jeong Tae Do

引用次数: 0

Assessment of Stem Cell Viability through Visual Analysis Coupled with Teachable Machine. 通过可视化分析结合可教机器评估干细胞活力。

IF 2.5 4区医学

International journal of stem cells Pub Date : 2025-06-09 DOI: 10.15283/ijsc24105

Chanhyung Kim, Jisu Son, Dinesh Chaudhary, Yeon-Kyun Park, Ji Hyeon Cho, Dongryeol Ryu, Jee-Heon Jeong, Jonghee Youn

{"title":"Assessment of Stem Cell Viability through Visual Analysis Coupled with Teachable Machine.","authors":"Chanhyung Kim, Jisu Son, Dinesh Chaudhary, Yeon-Kyun Park, Ji Hyeon Cho, Dongryeol Ryu, Jee-Heon Jeong, Jonghee Youn","doi":"10.15283/ijsc24105","DOIUrl":"https://doi.org/10.15283/ijsc24105","url":null,"abstract":"Cell viability is an indispensable aspect of cells in the field of drug discovery, cell biology, and biomedical research to assess the physiological conditions of cells such as healthiness, functionality, survivability, etc. Recently, there have been several methods for determining the cell viability through either cell staining with trypan blue and acridine orange, propidium iodide, calcein-AM, etc., or colorimetric assays such as cell counting kit-8 assay. However, these methods have some limitations like time-consuming, expensive, unstable, individual variability, etc. Even present artificial intelligence software such as QuPath, ImageJ, etc., can only determine the cell viability after cell staining. Therefore, we attempted to determine whether cells are alive or not depending on the visual characteristics of an individual cell using Teachable Machine, a web-based artificial intelligence tool provided by Google. Labeling work to assign correct answers to learning data consumes a lot of time and human costs because it is usually done manually. To solve this problem, labeling was automated by recognizing and extracting only individual cells from the image using the contour function to increase time efficiency. In addition, many datasets were created to evaluate and compare the performances of models. Based on the results, the model that showed the best performance showed an accuracy of more than 80%. In conclusion, this model could minimize analysis time, expenses, individual variability, etc., enhancing the efficacy and reproducibility of biological experiments in the fields of drug discovery, drug development, and biological research.","PeriodicalId":14392,"journal":{"name":"International journal of stem cells","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144247848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishment of a Human Induced Pluripotent Stem Cell-Derived Cerebral Cortex Organoid Model for Neurotoxicity Assessment. 人诱导多能干细胞衍生大脑皮层类器官模型的建立及其神经毒性评价。

IF 2.5 4区医学

International journal of stem cells Pub Date : 2025-06-09 DOI: 10.15283/ijsc24125

Seohee Park, Chang Hwan Yoon, Haeun Kim, Jiyou Han, Soo Hyun Nam, Kang Pa Lee, Sang-Hyuk Lee, Byung-Ok Choi, Jong Hyun Kim

{"title":"Establishment of a Human Induced Pluripotent Stem Cell-Derived Cerebral Cortex Organoid Model for Neurotoxicity Assessment.","authors":"Seohee Park, Chang Hwan Yoon, Haeun Kim, Jiyou Han, Soo Hyun Nam, Kang Pa Lee, Sang-Hyuk Lee, Byung-Ok Choi, Jong Hyun Kim","doi":"10.15283/ijsc24125","DOIUrl":"https://doi.org/10.15283/ijsc24125","url":null,"abstract":"Human pluripotent stem cell (hPSC)-derived brain organoids have emerged as innovative models for drug screening and cytotoxicity evaluation. However, their inherent cellular heterogeneity presents challenges in isolating targeted neuronal populations, such as upper motor neurons, which are crucial for motor cortex function. In this study, we developed motor cortex-like organoids enriched with excitatory glutamatergic and inhibitory GABAergic neurons to assess neurotoxicity in the upper motor neurons-a key component of voluntary motor control. By optimizing the differentiation protocols, we achieved robust expression of vGlut1 in excitatory neurons and GABA in inhibitory neurons by day 30 of the differentiation. The organoids were generated by co-culturing progenitor cells during the early differentiation phase, followed by lineage-specific maturation. Comparative analyses demonstrated that these organoids more accurately recapitulate the human cortical architecture than traditional neural cell line (SK-N-SH neuroblastoma cells). We observed that measures of cell viability and integrity-assessed via cleaved caspase-3 levels, growth-associated protein 43 (GAP43), and autophagy-related protein 5 (ATG5)-were significantly higher in 3D organoid cultures compared to conventional 2D systems. In toxicological assays, the motor cortex-like organoids exhibited a dose-dependent response to both toxic and non-toxic compounds, highlighting their potential as high-fidelity neurotoxicity screening models. Our findings suggest that hPSC-derived motor cortex-like organoids serve as a robust, physiologically relevant model that can replace animal models in toxicity assessments, offering enhanced accuracy in evaluating compounds that impact the motor cortex while reflecting better human brain physiology.","PeriodicalId":14392,"journal":{"name":"International journal of stem cells","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144247849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Temporal Regulation of Cytokines and Growth Factors for Optimized Hematopoietic-Lineage Specification from Human Pluripotent Stem Cells. 细胞因子和生长因子对人多能干细胞造血谱系优化的时间调控。

IF 2.5 4区医学

International journal of stem cells Pub Date : 2025-06-09 DOI: 10.15283/ijsc25020

Jisoo Park, Hyebin Koh, Eunsu Jeon, Kyungjun Uh, Sangjune Kim, Jong-Hee Lee

{"title":"Temporal Regulation of Cytokines and Growth Factors for Optimized Hematopoietic-Lineage Specification from Human Pluripotent Stem Cells.","authors":"Jisoo Park, Hyebin Koh, Eunsu Jeon, Kyungjun Uh, Sangjune Kim, Jong-Hee Lee","doi":"10.15283/ijsc25020","DOIUrl":"https://doi.org/10.15283/ijsc25020","url":null,"abstract":"Human pluripotent stem cells (hPSCs) can be used to investigate hematopoietic development and have the potential to advance cell-based therapies and to facilitate developmental biology studies. However, efficient ex vivo differentiation into hematopoietic lineages, including red blood cells (RBCs) of the erythroid lineage and immune cells such as macrophages of the myeloid lineage, is hampered by the need for precise temporal regulation of cytokines and growth factors. In this study, we developed an optimized protocol for hematopoietic lineage specification from hPSCs by fine-tuning the temporal dynamics of cytokine and growth factor applications. Prolonged mesodermal specification in the absence of hemogenic cytokines significantly enhanced the generation of hematopoietic progenitors (CD34＋CD45＋) with robust functional potential. Early administration of interleukin (IL)-3 during hematopoietic specification promoted progenitor expansion and maturation. Supplementation of bone morphogenetic protein 4 at the hematopoietic maturation stage enhanced the differentiation efficiency and preferentially drove myeloid lineage commitment toward macrophages at the expense of erythroid differentiation. The timing of erythropoietin administration was important in erythroid lineage commitment, and delayed treatment (day 10) enhanced erythroblast expansion and RBC production. By contrast, the timing of IL-6, GM-CSF, and M-CSF exposure did not significantly affect macrophage differentiation efficiency, suggesting that myeloid lineage specification follows a default pathway under optimized differentiation conditions. These findings suggest a refined, time-controlled strategy for directing hematopoietic differentiation from hPSCs, and provide insight into therapeutic blood cell production, regenerative medicine, and ex vivo modeling of hematopoietic disorders.","PeriodicalId":14392,"journal":{"name":"International journal of stem cells","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144247850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advanced Animal Replacement Testing Strategies Using Stem Cell and Organoids. 使用干细胞和类器官的先进动物替代试验策略。

IF 2.5 4区医学

International journal of stem cells Pub Date : 2025-05-30 Epub Date: 2025-03-11 DOI: 10.15283/ijsc24118

Chang-Jin Lee, Yoojun Nam, Yeri Alice Rim, Ji Hyeon Ju

{"title":"Advanced Animal Replacement Testing Strategies Using Stem Cell and Organoids.","authors":"Chang-Jin Lee, Yoojun Nam, Yeri Alice Rim, Ji Hyeon Ju","doi":"10.15283/ijsc24118","DOIUrl":"10.15283/ijsc24118","url":null,"abstract":"The increasing ethical concerns and regulatory restrictions surrounding animal testing have accelerated the development of advanced in vitro models that more accurately replicate human physiology. Among these, stem cell-based systems and organoids have emerged as revolutionary tools, providing ethical, scalable, and physiologically relevant alternatives. This review explores the key trends and driving factors behind the adoption of these models, such as technological advancements, the principles of the 3Rs (Replacement, Reduction, and Refinement), and growing regulatory support from agencies like the OECD and FDA. It also delves into the development and application of various model systems, including 3D reconstructed tissues, induced pluripotent stem cell-derived cells, and microphysiological systems, highlighting their potential to replace animal models in toxicity evaluation, disease modeling, and drug development. A critical aspect of implementing these models is ensuring robust quality control protocols to enhance reproducibility and standardization, which is necessary for gaining regulatory acceptance. Additionally, we discuss advanced strategies for assessing toxicity and efficacy, focusing on organ-specific evaluation methods and applications in diverse fields such as pharmaceuticals, cosmetics, and food safety. Despite existing challenges related to scalability, standardization, and regulatory alignment, these innovative models represent a transformative step towards reducing animal use and improving the relevance and reliability of preclinical testing outcomes.","PeriodicalId":14392,"journal":{"name":"International journal of stem cells","volume":" ","pages":"107-125"},"PeriodicalIF":2.5,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12122249/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143597061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Collagen Scaffold Augments the Therapeutic Effect of Human Umbilical Cord Mesenchymal Stem Cells in a Rat Model of Intrauterine Adhesion. 胶原支架增强人脐带间充质干细胞对大鼠宫内粘连模型的治疗作用。

IF 2.5 4区医学

International journal of stem cells Pub Date : 2025-05-30 Epub Date: 2025-02-25 DOI: 10.15283/ijsc24079

Linzhi Gao, Guifang Jiang, Enming Liang, Ying Zhang, Baoling Cheng, Xian Zhang, Dong Zhang, Xiaoyu Wang, Yuan Shen

{"title":"Collagen Scaffold Augments the Therapeutic Effect of Human Umbilical Cord Mesenchymal Stem Cells in a Rat Model of Intrauterine Adhesion.","authors":"Linzhi Gao, Guifang Jiang, Enming Liang, Ying Zhang, Baoling Cheng, Xian Zhang, Dong Zhang, Xiaoyu Wang, Yuan Shen","doi":"10.15283/ijsc24079","DOIUrl":"10.15283/ijsc24079","url":null,"abstract":"Intrauterine adhesion (IUA) caused by endometrial injury is the most common cause of female uterine infertility. Current treatments offer limited clinical benefits. In this study, we investigated the role of human umbilical cord mesenchymal stem cells (hUCMSCs) loaded collagen scaffold in the regeneration of injured human endometrium in an IUA rat model. Following the construction of the IUA rat model by mechanical injury, collagen scaffold, hUCMSCs, or hUCMSCs-loaded collagen scaffold was transplanted. The implantation of hUCMSCs-loaded collagen scaffold significantly increased the thickness of the endometrium, the number of endometrial glands and the abundance of blood vessels in IUA rats. Moreover, hUCMSCs-loaded collagen scaffold treatment significantly reduced endometrial fibrosis, increased the expression of Vegf, Integrin β3, Lif, and Igf-1, and finally improved endometrial receptivity in IUA rats. Taken together, our observations suggest that hUCMSCs-loaded collagen scaffold could be a practical therapeutic for treating IUA and restoring regeneration.","PeriodicalId":14392,"journal":{"name":"International journal of stem cells","volume":" ","pages":"135-145"},"PeriodicalIF":2.5,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12122250/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Dexamethasone and Tacrolimus on Mesenchymal Stem Cell Characteristics and Gene Expression. 地塞米松和他克莫司对间质干细胞特性和基因表达的影响

IF 2.5 4区医学

International journal of stem cells Pub Date : 2025-05-30 Epub Date: 2025-03-14 DOI: 10.15283/ijsc24116

Na Kyung Lee, Duk L Na, Su Hyeon Myeong, Seung-Yeon Lee, Na-Hee Lee, Hyemin Jang, Sang Won Seo, Jong Wook Chang, Hee Jin Kim, Hyo Jin Son

{"title":"Effects of Dexamethasone and Tacrolimus on Mesenchymal Stem Cell Characteristics and Gene Expression.","authors":"Na Kyung Lee, Duk L Na, Su Hyeon Myeong, Seung-Yeon Lee, Na-Hee Lee, Hyemin Jang, Sang Won Seo, Jong Wook Chang, Hee Jin Kim, Hyo Jin Son","doi":"10.15283/ijsc24116","DOIUrl":"10.15283/ijsc24116","url":null,"abstract":"Mesenchymal stem cells (MSCs) are frequently used for therapeutic applications in both pre-clinical and clinical settings owing to their capacity for immune modulation and neuroprotective effects. However, transient fever is commonly observed as an adverse event following MSC injection in patients with Alzheimer's disease (AD). In this study, we investigated the potential impact of immunosuppressants such as dexamethasone and tacrolimus on altering the characteristics of human mesenchymal stem cells (hMSCs). Additionally, we examined whether these immunosuppressants affect the persistence of hMSCs or the immune response upon their administration into the brain parenchyma of AD mice. The exposure of hMSCs to high concentrations of dexamethasone and tacrolimus in vitro did not significantly alter the characteristics of hMSCs. The expression of genes related to innate immune responses, such as Irak1, Irf3, Nod1, and Ifnar1, was significantly downregulated by the additional administration of dexamethasone and tacrolimus to the brain parenchyma of AD mice. However, hMSC persistence in the AD mouse brain was not affected. The results of this study support the use of immunosuppressants to mitigate fever during stem cell therapy in patients with AD.","PeriodicalId":14392,"journal":{"name":"International journal of stem cells","volume":" ","pages":"173-185"},"PeriodicalIF":2.5,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12122247/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143624738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Loss of Ubiquitin-Specific Protease 11 Mitigates Pulmonary Fibrosis in Human Pluripotent Stem Cell-Derived Alveolar Organoids. 泛素特异性蛋白酶11的缺失减轻了人多能干细胞衍生肺泡类器官的肺纤维化。

IF 2.5 4区医学

International journal of stem cells Pub Date : 2025-05-30 Epub Date: 2025-04-07 DOI: 10.15283/ijsc25011

Sripriya Rajkumar, Ji-Hye Jung, Ji-Young Kim, Janardhan Keshav Karapurkar, Girish Birappa, D A Ayush Gowda, C Bindu Ajaykumar, Haribalan Perumalsamy, Bharathi Suresh, Kye-Seong Kim, Seok-Ho Hong, Suresh Ramakrishna

{"title":"Loss of Ubiquitin-Specific Protease 11 Mitigates Pulmonary Fibrosis in Human Pluripotent Stem Cell-Derived Alveolar Organoids.","authors":"Sripriya Rajkumar, Ji-Hye Jung, Ji-Young Kim, Janardhan Keshav Karapurkar, Girish Birappa, D A Ayush Gowda, C Bindu Ajaykumar, Haribalan Perumalsamy, Bharathi Suresh, Kye-Seong Kim, Seok-Ho Hong, Suresh Ramakrishna","doi":"10.15283/ijsc25011","DOIUrl":"10.15283/ijsc25011","url":null,"abstract":"The etiology of chronic and lethal interstitial lung disease, termed idiopathic pulmonary fibrosis (IPF), remains unidentified. IPF induces pathological lung scarring that results in rigidity and impairs gas exchange, eventually resulting in premature mortality. Recent findings indicate that deubiquitinating enzymes play a key role in stabilizing fibrotic proteins and contribute to pulmonary fibrosis. The ubiquitin-specific protease 11 (USP11) promotes pro-fibrotic proteins, and its expression elevated in tissue samples from patients with IPF. Thus, this study aimed to examine the effects of loss of function of USP11 gene on the progression of pulmonary fibrosis by utilizing 3D cell culture alveolar organoids (AOs) that replicate the structure and functions of the proximal and distal airways and alveoli. Here, we applied the CRISPR/Cas9 system to knock out the USP11 gene in human induced pluripotent stem cells (hiPSCs) and then differentiated these hiPSCs into AOs. Loss of USP11 gene resulted in abnormalities in type 2 alveolar epithelial cells in the hiPSC-USP11KO-AOs. Moreover, knock out of the USP11 mitigates pulmonary fibrosis caused by TGF-β in hiPSC-USP11KO-AOs by reducing collagen formation and fibrotic markers, suggesting it has the therapeutic potential to treat IPF patients.","PeriodicalId":14392,"journal":{"name":"International journal of stem cells","volume":" ","pages":"205-213"},"PeriodicalIF":2.5,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12122244/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143795330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mammalian Blastema: Possibility and Potentials. 哺乳动物胚芽：可能性和潜力。

IF 2.5 4区医学

International journal of stem cells Pub Date : 2025-05-30 Epub Date: 2025-03-10 DOI: 10.15283/ijsc24121

Juhyeon Nam, Byungkuk Min, Areum Baek, Sang-Yun Lee, Jeongmin Ha, Min Ji Cho, Janghwan Kim

{"title":"Mammalian Blastema: Possibility and Potentials.","authors":"Juhyeon Nam, Byungkuk Min, Areum Baek, Sang-Yun Lee, Jeongmin Ha, Min Ji Cho, Janghwan Kim","doi":"10.15283/ijsc24121","DOIUrl":"10.15283/ijsc24121","url":null,"abstract":"Regeneration is a process that restores the structure and function of injured tissues or organs. Regenerative capacities vary significantly across species, with amphibians and fish demonstrating a high regenerative capacity even after severe injuries. This capacity is largely attributed to the formation of a blastema, a mass of multipotent cells reprogrammed from differentiated cells at the injury site. In contrast, mammals exhibit limited regenerative capacities, with blastema- like cells forming only in specific contexts, such as antler or digit tip regeneration. An interesting aspect of blastema formation in highly regenerative organisms is the temporary expression of pluripotency factors as known as the Yamanaka factors (YFs), which is a key requirement for reprogramming somatic cells into induced pluripotent stem cells (iPSCs). While iPSCs hold pros and cons, direct or partial reprogramming with YF has been proposed as a safer alternative. Since blastema formation and partial reprogramming are similar in terms of YF expressions, we found blastema-like cells in mammalian reprogramming with YF. This review outlines the characteristics of blastema across various organisms, emphasizing interspecies differences. We also explore studies on partial reprogramming and the possibility of inducing blastema-like cells via the temporary expression of YF in mammals.","PeriodicalId":14392,"journal":{"name":"International journal of stem cells","volume":" ","pages":"126-134"},"PeriodicalIF":2.5,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12122245/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143585406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0