International journal of stem cells最新文献

Unraveling Optimal Dose and Responsive Markers for Human Cord Blood Derived Mononuclear Cells in Alleviating Bronchopulmonary Dysplasia in Neonatal Mice. 揭示人脐带血源性单核细胞缓解新生小鼠支气管肺发育不良的最佳剂量和反应性标志物。

IF 2.4 4区医学

International journal of stem cells Pub Date : 2025-09-12 DOI: 10.15283/ijsc25008

Jia Chen, Yuhan Chen, Xue Du, Liu He, Yanhua Wang, Huiling Han, Qiuping Li, Huixia Zhou, Zhichun Feng

{"title":"Unraveling Optimal Dose and Responsive Markers for Human Cord Blood Derived Mononuclear Cells in Alleviating Bronchopulmonary Dysplasia in Neonatal Mice.","authors":"Jia Chen, Yuhan Chen, Xue Du, Liu He, Yanhua Wang, Huiling Han, Qiuping Li, Huixia Zhou, Zhichun Feng","doi":"10.15283/ijsc25008","DOIUrl":"https://doi.org/10.15283/ijsc25008","url":null,"abstract":"Bronchopulmonary dysplasia (BPD), characterized by impaired alveolarization and dysregulated vascularization, is a severe health burden for neonates worldwide. Hyperoxia induced acute lung injury is a major contributor to the progression and deterioration of BPD. An increasing number of animal studies have revealed that human umbilical cord blood derived mononuclear cells (hUCB-MNCs) infusion significantly attenuated the hyperoxia-induced acute lung injury through regeneration capacity. Currently, clinical application requires determination of the optimal dose and adjustment to good manufacturing practices. In this work, we comprehensively investigated the optimal dose of hUCB-MNCs in alleviating hyperoxia-induced lung injury in neonatal C57BL6/J mice. Mice with hyperoxia exposure were implanted with low (3×104 cells/kg, Dl), middle (3×105 cells/kg, Dm) and high (3×106 cells/kg, Dh) dose of hUCB-MNCs at postnatal day 7. Three weeks after graft, characteristics exhibited in lungs including morphology, function and cytokine expression were thoroughly analyzed. Implantation of hUCB-MNCs sharply reverted the impaired lung architecture induced by hyperoxia exposure dose dependently as evidenced by indicated parameters. Attenuated expression of IL-1β concomitant with enhanced expression of IL-10 and IL-2 were shown in Dh inoculated groups, where Dl and Dm failed to restore the level of IL-10, IL-1β and IL-2. Significantly re-escalated marker of angiogenic marker VEGFA, CD31 in lung tissue were uniquely observed in Dh group. Mechanistically, our study revealed the appropriate dose of intravenous infusion of hUCB-MNCs in alleviating hyperoxia-induced lung injury through modulating reactive oxygen species response in neonatal mice. Therefore, a tight control of hUCB-MNCs density or levels of CB-MNC related products is of great significance.","PeriodicalId":14392,"journal":{"name":"International journal of stem cells","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145040147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Organoid Technology as a Promising Frontier in Organ Transplantation and Regenerative Medicine. 类器官技术是器官移植和再生医学的一个有前途的前沿。

IF 2.4 4区医学

International journal of stem cells Pub Date : 2025-09-12 DOI: 10.15283/ijsc25042

Aeri Shin, Min Kyu Yum, Woong Sun

{"title":"Organoid Technology as a Promising Frontier in Organ Transplantation and Regenerative Medicine.","authors":"Aeri Shin, Min Kyu Yum, Woong Sun","doi":"10.15283/ijsc25042","DOIUrl":"https://doi.org/10.15283/ijsc25042","url":null,"abstract":"The advent of medical advances has resulted in the development of an array of treatments aimed at restoring damaged organs in humans. However, when chemical treatments, such as drug therapies, are constrained, organ transplantation may ultimately emerge as the sole viable solution. Nevertheless, despite the continually increasing demand for organ donations, the actual number of donated organs remains insufficient to meet this demand. Recently, a variety of organoids have been generated using stem cells and have been demonstrated to exhibit functionality comparable to that of native organs. This indicates that organoids may be a viable option for use in organ transplantation. However, while numerous recent publications have documented the regenerative effects of diverse organoid types when implanted into damaged regions, significant technical and ethical considerations must be addressed before organoids can be utilized as a replacement for human organs. This review presents an overview of experimental endeavors in regenerative therapies through organoid transplantation, while also addressing the challenges that must be overcome to enhance the feasibility of organoid use as a surrogate organ. As organoid technology continues to advance, organoids may eventually become a widely utilized surrogate source for organ replacement in clinical settings.","PeriodicalId":14392,"journal":{"name":"International journal of stem cells","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145040201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances in Cell and Gene Therapy for Rare Disease Treatment. 罕见病的细胞和基因治疗进展。

IF 2.4 4区医学

International journal of stem cells Pub Date : 2025-09-01 DOI: 10.15283/ijsc25028

Hyun Jin Baek, Yoojun Nam, Yeri Alice Rim, Ji Hyeon Ju

{"title":"Advances in Cell and Gene Therapy for Rare Disease Treatment.","authors":"Hyun Jin Baek, Yoojun Nam, Yeri Alice Rim, Ji Hyeon Ju","doi":"10.15283/ijsc25028","DOIUrl":"https://doi.org/10.15283/ijsc25028","url":null,"abstract":"A rare disease is generally defined as a medical condition that affects a small proportion of the population, though specific thresholds vary across countries. Despite regional differences, these definitions consistently reflect the low prevalence of such conditions, the limited availability of effective treatments, and the pressing need for targeted research and regulatory support. As a result of their rarity and low commercial potential, rare diseases have historically represented an area of market failure, where investment and research have been limited and often neglected. However, since the 1990s, each country has guaranteed continuous support to research and development projects to promote the advancements of rare disease treatments, achieving a growth rate greater than that of the entire pharmaceutical industry. In this review, we examine the status of orphan drug development using an advanced therapy medicinal product (ATMP) approach in the growing rare disease market, with a particular focus on cell therapies and gene therapies, which constitute the most actively developed and clinically applied categories within ATMPs. We also explore strategic approaches through which the orphan drug industry can utilize ATMPs, especially these two modalities, to enhance its competitiveness.","PeriodicalId":14392,"journal":{"name":"International journal of stem cells","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144954181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional Validation of Alcohol Dependence-Associated FYN Variants Using Gene Editing and Stem Cell Study Approaches. 使用基因编辑和干细胞研究方法对酒精依赖相关FYN变异的功能验证

IF 2.4 4区医学

International journal of stem cells Pub Date : 2025-08-30 Epub Date: 2025-04-10 DOI: 10.15283/ijsc24123

Bilal Cakir, Yoshiaki Tanaka, Mu Seog Choe, Pei-Yu Chu, Yangfei Xiang, Kun-Yong Kim, Mei Zhong, Joel Gelernter, Huiping Zhang, John Krystal, In-Hyun Park

{"title":"Functional Validation of Alcohol Dependence-Associated FYN Variants Using Gene Editing and Stem Cell Study Approaches.","authors":"Bilal Cakir, Yoshiaki Tanaka, Mu Seog Choe, Pei-Yu Chu, Yangfei Xiang, Kun-Yong Kim, Mei Zhong, Joel Gelernter, Huiping Zhang, John Krystal, In-Hyun Park","doi":"10.15283/ijsc24123","DOIUrl":"10.15283/ijsc24123","url":null,"abstract":"Alcohol dependence (AD) is one of the most prevalent neuropsychiatric disorders. Multiple polymorphisms in the Fyn tyrosine kinase gene (FYN) were found to be associated with AD. The function of AD-associated FYN variants remains largely unknown due to the absence of an appropriate model for studying them. Here, we generated human embryonic stem cell lines homozygous/heterozygous for rs706895 C/T alleles in 5' untranslated region (5' UTR) of FYN by CRISPR-Cas9 editing to explore the AD association. Transcriptome and reporter gene analyses demonstrated that induced neurons with the rs706895 C allele showed a significantly higher expression level of FYN under ethanol treatment. Our results suggest that FYN 5' UTR variant rs706895 may influence an individual's vulnerability to AD by altering FYN expression. Targeting AD-associated variants may provide a better understanding of disease mechanisms and a reliable basis for the personalized AD treatment.","PeriodicalId":14392,"journal":{"name":"International journal of stem cells","volume":" ","pages":"263-274"},"PeriodicalIF":2.4,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12394083/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143988041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishment of a Human Induced Pluripotent Stem Cell-Derived Cerebral Cortex Organoid Model for Neurotoxicity Assessment. 人诱导多能干细胞衍生大脑皮层类器官模型的建立及其神经毒性评价。

IF 2.4 4区医学

International journal of stem cells Pub Date : 2025-08-30 Epub Date: 2025-06-09 DOI: 10.15283/ijsc24125

Seohee Park, Chang-Whan Yoon, Haeun Kim, Jiyou Han, Soo Hyun Nam, Kang Pa Lee, Sang-Hyuk Lee, Byung-Ok Choi, Jong Hyun Kim

{"title":"Establishment of a Human Induced Pluripotent Stem Cell-Derived Cerebral Cortex Organoid Model for Neurotoxicity Assessment.","authors":"Seohee Park, Chang-Whan Yoon, Haeun Kim, Jiyou Han, Soo Hyun Nam, Kang Pa Lee, Sang-Hyuk Lee, Byung-Ok Choi, Jong Hyun Kim","doi":"10.15283/ijsc24125","DOIUrl":"10.15283/ijsc24125","url":null,"abstract":"Human pluripotent stem cell (hPSC)-derived brain organoids have emerged as innovative models for drug screening and cytotoxicity evaluation. However, their inherent cellular heterogeneity presents challenges in isolating targeted neuronal populations, such as upper motor neurons, which are crucial for motor cortex function. In this study, we developed motor cortex-like organoids enriched with excitatory glutamatergic and inhibitory GABAergic neurons to assess neurotoxicity in the upper motor neurons-a key component of voluntary motor control. By optimizing the differentiation protocols, we achieved robust expression of vGlut1 in excitatory neurons and GABA in inhibitory neurons by day 30 of the differentiation. The organoids were generated by co-culturing progenitor cells during the early differentiation phase, followed by lineage-specific maturation. Comparative analyses demonstrated that these organoids more accurately recapitulate the human cortical architecture than traditional neural cell line (SK-N-SH neuroblastoma cells). We observed that measures of cell viability and integrity-assessed via cleaved caspase-3 levels, growth-associated protein 43 (GAP43), and autophagy-related protein 5 (ATG5)-were significantly higher in 3D organoid cultures compared to conventional 2D systems. In toxicological assays, the motor cortex-like organoids exhibited a dose-dependent response to both toxic and non-toxic compounds, highlighting their potential as high-fidelity neurotoxicity screening models. Our findings suggest that hPSC-derived motor cortex-like organoids serve as a robust, physiologically relevant model that can replace animal models in toxicity assessments, offering enhanced accuracy in evaluating compounds that impact the motor cortex while reflecting better human brain physiology.","PeriodicalId":14392,"journal":{"name":"International journal of stem cells","volume":" ","pages":"275-285"},"PeriodicalIF":2.4,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12394079/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144247849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Beyond Structure: Next-Generation Electrophysiological Platforms for Functional Brain Organoids. 超越结构：功能性脑类器官的下一代电生理平台。

IF 2.4 4区医学

International journal of stem cells Pub Date : 2025-08-30 Epub Date: 2025-07-31 DOI: 10.15283/ijsc25056

Ji-Hyoung Cha, Keuntae Kim, Il-Joo Cho

{"title":"Beyond Structure: Next-Generation Electrophysiological Platforms for Functional Brain Organoids.","authors":"Ji-Hyoung Cha, Keuntae Kim, Il-Joo Cho","doi":"10.15283/ijsc25056","DOIUrl":"10.15283/ijsc25056","url":null,"abstract":"Brain organoids have emerged as transformative models for studying human neurodevelopment, neurological disorders, and personalized therapeutics. Central to their utility is the ability to monitor neural activity with high spatial and temporal resolution. Traditional electrophysiological tools-such as planar microelectrode arrays and patch-clamp techniques-offer limited access to the three-dimensional and dynamic nature of organoid neural networks. Recent technological advancements have led to the development of next-generation platforms including surface-embedded, flexible, and fully implantable electrodes. Moreover, multifunctional probes incorporating optical, chemical, and mechanical sensing open new avenues for multimodal interrogation of organoid physiology. This review summarizes the current state of electrophysiological technologies applied to brain organoids, highlighting innovations in recording fidelity, spatiotemporal resolution, and device-tissue integration. We also discuss key challenges such as maintaining organoid viability, achieving sufficient electrode density, and enabling non-disruptive, chronic interfacing throughout organoid development. Looking forward, future systems are expected to evolve toward ultra-dense, multimodal, and closed-loop interfaces capable of investigating organoid function throughout extended growth periods. These advances will not only deepen our understanding of brain-like activity in organoids but also support the design of more functionally accurate and translationally relevant neural models.","PeriodicalId":14392,"journal":{"name":"International journal of stem cells","volume":" ","pages":"215-236"},"PeriodicalIF":2.4,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12394082/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144753326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of Stem Cell Viability through Visual Analysis Coupled with Teachable Machine. 通过可视化分析结合可教机器评估干细胞活力。

IF 2.4 4区医学

International journal of stem cells Pub Date : 2025-08-30 Epub Date: 2025-06-09 DOI: 10.15283/ijsc24105

Chanhyung Kim, Jisu Son, Dinesh Chaudhary, Yeon-Kyun Park, Ji Hyeon Cho, Dongryeol Ryu, Jee-Heon Jeong, Jonghee Youn

{"title":"Assessment of Stem Cell Viability through Visual Analysis Coupled with Teachable Machine.","authors":"Chanhyung Kim, Jisu Son, Dinesh Chaudhary, Yeon-Kyun Park, Ji Hyeon Cho, Dongryeol Ryu, Jee-Heon Jeong, Jonghee Youn","doi":"10.15283/ijsc24105","DOIUrl":"10.15283/ijsc24105","url":null,"abstract":"Cell viability is an indispensable aspect of cells in the field of drug discovery, cell biology, and biomedical research to assess the physiological conditions of cells such as healthiness, functionality, survivability, etc. Recently, there have been several methods for determining the cell viability through either cell staining with trypan blue and acridine orange, propidium iodide, calcein-AM, etc., or colorimetric assays such as cell counting kit-8 assay. However, these methods have some limitations like time-consuming, expensive, unstable, individual variability, etc. Even present artificial intelligence software such as QuPath, ImageJ, etc., can only determine the cell viability after cell staining. Therefore, we attempted to determine whether cells are alive or not depending on the visual characteristics of an individual cell using Teachable Machine, a web-based artificial intelligence tool provided by Google. Labeling work to assign correct answers to learning data consumes a lot of time and human costs because it is usually done manually. To solve this problem, labeling was automated by recognizing and extracting only individual cells from the image using the contour function to increase time efficiency. In addition, many datasets were created to evaluate and compare the performances of models. Based on the results, the model that showed the best performance showed an accuracy of more than 80%. In conclusion, this model could minimize analysis time, expenses, individual variability, etc., enhancing the efficacy and reproducibility of biological experiments in the fields of drug discovery, drug development, and biological research.","PeriodicalId":14392,"journal":{"name":"International journal of stem cells","volume":" ","pages":"311-319"},"PeriodicalIF":2.4,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12394080/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144247848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Phase 1/2a Clinical Trial to Evaluate the Efficacy and Safety of Allogenic Adipose Tissue-Derived Mesenchymal Stem Cell Clusters in Patients with Critical Limb Ischemia. 一项评估同种异体脂肪组织来源的间充质干细胞簇在重度肢体缺血患者中的有效性和安全性的1/2a期临床试验

IF 2.4 4区医学

International journal of stem cells Pub Date : 2025-08-30 Epub Date: 2025-03-17 DOI: 10.15283/ijsc25002

Yong-Man Park, Joon-Kee Park, Hyo-Shin Kim, Shin-Seok Yang, Jong-Wan Kim, Dong-Ik Kim

{"title":"A Phase 1/2a Clinical Trial to Evaluate the Efficacy and Safety of Allogenic Adipose Tissue-Derived Mesenchymal Stem Cell Clusters in Patients with Critical Limb Ischemia.","authors":"Yong-Man Park, Joon-Kee Park, Hyo-Shin Kim, Shin-Seok Yang, Jong-Wan Kim, Dong-Ik Kim","doi":"10.15283/ijsc25002","DOIUrl":"10.15283/ijsc25002","url":null,"abstract":"Critical limb ischemia (CLI) is a severe manifestation of peripheral arterial disease, often resulting in ischemic rest pain, non-healing ulcers, or gangrene. Due to the limited effectiveness of conventional revascularization techniques in 5%∼20% of patients, alternative therapeutic approaches are needed. This Phase 1/2a clinical trial evaluated the safety, tolerability, and efficacy of allogeneic adipose tissue-derived mesenchymal stem cell clusters (ADMSCCs) in patients with CLI who were not eligible for standard revascularization methods. The study was conducted in two phases: Phase 1 used a 3＋3 dose-escalation design to determine tolerability, and Phase 2a assessed efficacy at the maximum tolerated dose. Twenty patients were treated with ADMSCCs, with safety (adverse events and dose-limiting toxicity) and efficacy (pain intensity, walking distance, and ulcer size) as primary endpoints. ADMSCCs were injected intramuscularly, and patients were monitored for 24 weeks. ADMSCCs were well-tolerated, with no serious adverse events or dose-limiting toxicities observed. Significant reductions in ischemic pain and increases in pain-free walking distance were noted at 4, 12, and 24 weeks. Although ankle-brachial index and toe-brachial index showed no significant changes, ulcer healing was observed in one participant. These findings suggest that ADMSCC therapy may be a viable alternative for patients with CLI, with a favorable safety profile and sustained therapeutic effects. Further studies with larger sample sizes and randomized control groups are needed to confirm these results and explore integration with existing treatments.","PeriodicalId":14392,"journal":{"name":"International journal of stem cells","volume":" ","pages":"254-262"},"PeriodicalIF":2.4,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12394084/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143648508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extracellular Vesicles from Induced Mesenchymal Stem Cells Inhibit Acute Kidney Injury to Chronic Kidney Disease Transition. 间充质干细胞诱导的细胞外囊泡抑制急性肾损伤向慢性肾病的转变。

IF 2.4 4区医学

International journal of stem cells Pub Date : 2025-08-30 Epub Date: 2025-03-14 DOI: 10.15283/ijsc24127

Hongduk Kim, Sungok Hong, Soo Kim, Tae Min Kim

{"title":"Extracellular Vesicles from Induced Mesenchymal Stem Cells Inhibit Acute Kidney Injury to Chronic Kidney Disease Transition.","authors":"Hongduk Kim, Sungok Hong, Soo Kim, Tae Min Kim","doi":"10.15283/ijsc24127","DOIUrl":"10.15283/ijsc24127","url":null,"abstract":"Compared with conventional mesenchymal stem cells (MSCs), induced mesenchymal stem cells (iMSCs) from induced pluripotent stem cells are unique cell sources for tissue regeneration. The effect of extracellular vesicles (EVs) secreted from iMSCs on inhibiting acute kidney injury (AKI) to chronic kidney disease (CKD) transition was not reported. In this study, we investigated whether EVs from iMSCs (iMSC-EVs) could inhibit AKI-to-CKD transition. iMSC-EVs exhibited the general characteristics of EVs, such as protein marker expression, morphology, and size. Additionally, iMSC-EVs were detected in renal tissues after intravenous injection. In human renal tubular epithelial cells, the increase in pro-fibrotic gene expression in response to transforming growth factor β1 treatment was decreased by iMSC-EVs. In a mouse model of the AKI-to-CKD transtion induced by folic acid, repeated administration of iMSC-EVs restored renal function at day 14. Specifically, iMSC-EVs reduced interstitial fibrosis, sustained inflammation, various types of cell death, and the number of immune cells infiltrating kidneys. Capillary rarefaction in renal tissue was also reversed by iMSC-EVs. Our results demonstrate that iMSC-EVs reduced interstitial fibrosis, inflammation, and cell death occurring during the CKD transition after AKI. Thus, iMSC-EVs have the potential to block AKI-to-CKD transition.","PeriodicalId":14392,"journal":{"name":"International journal of stem cells","volume":" ","pages":"286-300"},"PeriodicalIF":2.4,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12394081/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143624741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Thermostable bFGF Improves Cell Lifespan by Enhancing Cell Activity in the Long-Term Culture of Human Orbicularis Oculi Stem Cells. 在人眼轮匝肌干细胞的长期培养过程中，恒温 bFGF 可通过增强细胞活性来延长细胞寿命。

IF 2.4 4区医学

International journal of stem cells Pub Date : 2025-08-30 Epub Date: 2024-10-23 DOI: 10.15283/ijsc24039

Geun-Ho Kang, Yeo Kyung Shin, Kyung Min Lim, Se Jong Kim, Myeongjin Song, Kwonwoo Song, Jung Hyun Kim, Dae Young Kim, Hang-Cheol Shin, Hyun Jin Shin, Ssang-Goo Cho

{"title":"Thermostable bFGF Improves Cell Lifespan by Enhancing Cell Activity in the Long-Term Culture of Human Orbicularis Oculi Stem Cells.","authors":"Geun-Ho Kang, Yeo Kyung Shin, Kyung Min Lim, Se Jong Kim, Myeongjin Song, Kwonwoo Song, Jung Hyun Kim, Dae Young Kim, Hang-Cheol Shin, Hyun Jin Shin, Ssang-Goo Cho","doi":"10.15283/ijsc24039","DOIUrl":"10.15283/ijsc24039","url":null,"abstract":"Stem cells derived from human orbicularis oculi muscle (hOOM) are a valuable resource for cell therapy. However, when stem cells are continuously cultured, their abilities tend to deteriorate over time. One method to address this issue is to use basic fibroblast growth factor (bFGF) to maintain the stem cell functionality. The limitation is that bFGF is unstable under mammalian cell culture conditions with a half-life of only 8 hours, which poses a significant challenge to the production and maintenance of high-quality stem cells. In this study, we used thermostable bFGF (TS-bFGF) and demonstrated that hOOM-derived stem cells cultured with TS-bFGF exhibited superior proliferation, stem cell function, reduced reactive oxygen species, and cellular senescence delay effect compared to cells cultured with wild-type bFGF. Considering the pivotal role of stem cells in broad ranges of applications such as regenerative medicine and cultured meat, we anticipate that TS-bFGF, owing to its thermostability and long-lasting properties, will contribute significantly to the acquisition of high-quality stem cells.","PeriodicalId":14392,"journal":{"name":"International journal of stem cells","volume":" ","pages":"301-310"},"PeriodicalIF":2.4,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12394077/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142500566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0