International journal of stem cells最新文献_第2页

The Differential Developmental Neurotoxicity of Valproic Acid on Anterior and Posterior Neural Induction of Human Pluripotent Stem Cells. 丙戊酸对人类多能干细胞前部和后部神经诱导的不同发育神经毒性。

IF 2.5 4区医学

International journal of stem cells Pub Date : 2025-02-28 Epub Date: 2024-07-08 DOI: 10.15283/ijsc24066

Jeongah Kim, Si-Hyung Park, Woong Sun

{"title":"The Differential Developmental Neurotoxicity of Valproic Acid on Anterior and Posterior Neural Induction of Human Pluripotent Stem Cells.","authors":"Jeongah Kim, Si-Hyung Park, Woong Sun","doi":"10.15283/ijsc24066","DOIUrl":"10.15283/ijsc24066","url":null,"abstract":"Valproic acid (VPA), widely used as an antiepileptic drug, exhibits developmental neurotoxicity when exposure occurs during early or late pregnancy, resulting in various conditions ranging from neural tube defects to autism spectrum disorders. However, toxicity during the very early stages of neural development has not been addressed. Therefore, we investigated the effects of VPA in a model where human pluripotent stem cells differentiate into anterior or posterior neural tissues. Exposure to VPA during the induction of neural stem cells induced different developmental toxic effects in a dose-dependent manner. For instance, VPA induced cell death more profoundly during anteriorly guided neural progenitor induction, while inhibition of cell proliferation and enhanced differentiation were observed during posteriorly guided neural induction. Furthermore, acute exposure to VPA during the posterior induction step also retarded the subsequent neurulation-like tube morphogenesis process in neural organoid culture. These results suggest that VPA exposure during very early embryonic development might exhibit cytotoxicity and subsequently disrupt neural differentiation and morphogenesis processes.","PeriodicalId":14392,"journal":{"name":"International journal of stem cells","volume":" ","pages":"49-58"},"PeriodicalIF":2.5,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11867903/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141554758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immune-Epithelial Cell Interactions during Epidermal Regeneration, Repair, and Inflammatory Diseases. 表皮再生、修复和炎症性疾病过程中免疫与上皮细胞的相互作用

IF 2.5 4区医学

International journal of stem cells Pub Date : 2025-02-28 Epub Date: 2024-01-09 DOI: 10.15283/ijsc23107

Axel D Schmitter-Sánchez, Sangbum Park

引用次数: 0

Crosstalk between Signaling Pathways and Energy Metabolism in Pluripotency. 多能性中信号通路与能量代谢之间的相互影响

IF 2.5 4区医学

International journal of stem cells Pub Date : 2025-02-28 Epub Date: 2024-03-18 DOI: 10.15283/ijsc23173

Keun-Tae Kim, Seong-Min Kim, Hyuk-Jin Cha

引用次数: 0

The Effect of Nerve Growth Factor on Cartilage Fibrosis and Hypertrophy during In Vitro Chondrogenesis Using Induced Pluripotent Stem Cells. 神经生长因子对体外诱导多能干细胞成软骨过程中软骨纤维化和肥大的影响。

IF 2.5 4区医学

International journal of stem cells Pub Date : 2025-02-28 Epub Date: 2024-12-30 DOI: 10.15283/ijsc24097

Se In Jung, Si Hwa Choi, Jang-Woon Kim, Jooyoung Lim, Yeri Alice Rim, Ji Hyeon Ju

{"title":"The Effect of Nerve Growth Factor on Cartilage Fibrosis and Hypertrophy during In Vitro Chondrogenesis Using Induced Pluripotent Stem Cells.","authors":"Se In Jung, Si Hwa Choi, Jang-Woon Kim, Jooyoung Lim, Yeri Alice Rim, Ji Hyeon Ju","doi":"10.15283/ijsc24097","DOIUrl":"10.15283/ijsc24097","url":null,"abstract":"Nerve growth factor (NGF) is a neurotrophic factor usually involved in the survival, differentiation, and growth of sensory neurons and nociceptive function. Yet, it has been suggested to play a role in the pathogenesis of osteoarthritis (OA). Previous studies suggested a possible relationship between NGF and OA; however, the underlying mechanisms remain unknown. Therefore, we investigated the impact of NGF in chondrogenesis using human induced pluripotent stem cells (hiPSCs)-derived chondrogenic pellets. To investigate how NGF affects the cartilage tissue, hiPSC-derived chondrogenic pellets were treated with NGF on day 3 of differentiation, expression of chondrogenic, hypertrophic, and fibrotic markers was confirmed. Also, inflammatory cytokine arrays were performed using the culture medium of the NGF treated chondrogenic pellets. As a result, NGF treatment decreased the expression of pro-chondrogenic markers by approximately 2~4 times, and hypertrophic (pro-osteogenic) markers and fibrotic markers were increased by approximately 3-fold or more in the NGF-treated cartilaginous pellets. In addition, angiogenesis was upregulated by approximately 4-fold or more, bone formation by more than 2-fold, and matrix metalloproteinase induction by more than 2-fold. These inflammatory cytokine array were using the NGF-treated chondrogenic pellet cultured medium. Furthermore, it was confirmed by Western blot to be related to the induction of the glycogen synthase kinase-3 beta (GSK3β) pathway by NGF. In Conclusions, these findings provide valuable insights into the multifaceted role of NGF in cartilage hypertrophy and fibrosis, which might play a critical role in OA progression.","PeriodicalId":14392,"journal":{"name":"International journal of stem cells","volume":" ","pages":"59-71"},"PeriodicalIF":2.5,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11867901/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142903010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Collagen Scaffold Augments the Therapeutic Effect of Human Umbilical Cord Mesenchymal Stem Cells in a Rat Model of Intrauterine Adhesion.

IF 2.5 4区医学

International journal of stem cells Pub Date : 2025-02-25 DOI: 10.15283/ijsc24079

Linzhi Gao, Guifang Jiang, Enming Liang, Ying Zhang, Baoling Cheng, Xian Zhang, Dong Zhang, Xiaoyu Wang, Yuan Shen

{"title":"Collagen Scaffold Augments the Therapeutic Effect of Human Umbilical Cord Mesenchymal Stem Cells in a Rat Model of Intrauterine Adhesion.","authors":"Linzhi Gao, Guifang Jiang, Enming Liang, Ying Zhang, Baoling Cheng, Xian Zhang, Dong Zhang, Xiaoyu Wang, Yuan Shen","doi":"10.15283/ijsc24079","DOIUrl":"https://doi.org/10.15283/ijsc24079","url":null,"abstract":"Intrauterine adhesion (IUA) caused by endometrial injury is the most common cause of female uterine infertility. Current treatments offer limited clinical benefits. In this study, we investigated the role of human umbilical cord mesenchymal stem cells (hUCMSCs) loaded collagen scaffold in the regeneration of injured human endometrium in an IUA rat model. Following the construction of the IUA rat model by mechanical injury, collagen scaffold, hUCMSCs, or hUCMSCs-loaded collagen scaffold was transplanted. The implantation of hUCMSCs-loaded collagen scaffold significantly increased the thickness of the endometrium, the number of endometrial glands and the abundance of blood vessels in IUA rats. Moreover, hUCMSCs-loaded collagen scaffold treatment significantly reduced endometrial fibrosis, increased the expression of Vegf, Integrin β3, Lif, and Igf-1, and finally improved endometrial receptivity in IUA rats. Taken together, our observations suggest that hUCMSCs-loaded collagen scaffold could be a practical therapeutic for treating IUA and restoring regeneration.","PeriodicalId":14392,"journal":{"name":"International journal of stem cells","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mesenchymal Stem Cells Mediated Suppression of GREM2 Inhibits Renal Epithelial-Mesenchymal Transition and Attenuates the Progression of Diabetic Kidney Disease. 间充质干细胞介导的GREM2抑制肾上皮-间充质转化并减缓糖尿病肾病的进展

IF 2.5 4区医学

International journal of stem cells Pub Date : 2025-01-06 DOI: 10.15283/ijsc24113

Myoung Seok Ko, Ji-Young Yun, Serin Kim, Mi-Ok Kim, Sang-Hyeok Go, Hye Jin Jin, Eun Hee Koh

{"title":"Mesenchymal Stem Cells Mediated Suppression of GREM2 Inhibits Renal Epithelial-Mesenchymal Transition and Attenuates the Progression of Diabetic Kidney Disease.","authors":"Myoung Seok Ko, Ji-Young Yun, Serin Kim, Mi-Ok Kim, Sang-Hyeok Go, Hye Jin Jin, Eun Hee Koh","doi":"10.15283/ijsc24113","DOIUrl":"https://doi.org/10.15283/ijsc24113","url":null,"abstract":"Diabetic kidney disease (DKD) is the leading cause of end-stage renal disease worldwide. Despite advancements in various treatments, the prevalence of DKD continues to rise, leading to a significant increase in the demand for dialysis and kidney transplantation. This study aimed to evaluate the effects of a Small cell＋Ultra Potent＋Scale UP cell (SMUP-Cell), a type of human umbilical cord blood-derived mesenchymal stem cell, on DKD in the db/db mouse model of type 2 diabetes mellitus. After administering SMUP-Cells via tail vein injection in db/db mice, the animals were monitored over a three-month period. The db/db mice exhibited an increased urine albumin-to-creatinine ratio (UACR). However, the administration of SMUP-Cells resulted in a reduction of the UACR. The expression levels of desmin, α-smooth muscle actin, and fibronectin-markers of epithelial-mesenchymal transition (EMT)-as well as kidney injury molecule 1, a sensitive marker of tubular injury, were significantly elevated in db/db mice. Treatment with SMUP-Cells ameliorated all of these changes. Notably, Gremlin isoform 2 (Grem2) exhibited the most significant difference in expression according to the transcriptome analysis. The elevated expression of Grem2 in db/db mice was significantly reduced following SMUP-Cell treatment. In vitro, treatment with high glucose and cholesterol induced Grem2 expression in renal tubular epithelial cells (RTECs), while Grem2 knockdown effectively prevented fibrosis and senescence induced by high glucose and cholesterol in RTECs. These observations suggest that SMUP-Cells inhibit the progression of DKD by inhibiting EMT through the reduction of Grem2 expression in RTECs.","PeriodicalId":14392,"journal":{"name":"International journal of stem cells","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142930935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Characterization and Regulation of Schwann Cells in the Tooth Germ Development and Odontogenic Differentiation. 雪旺细胞在牙胚发育和牙源性分化过程中的特征和调控。

IF 2.5 4区医学

International journal of stem cells Pub Date : 2024-11-30 Epub Date: 2024-07-29 DOI: 10.15283/ijsc23205

Jing He, Ting Wang, Danyang Liu, Jun Yang, Yuanpei He, Shouliang Zhao, Yanqin Ju

{"title":"The Characterization and Regulation of Schwann Cells in the Tooth Germ Development and Odontogenic Differentiation.","authors":"Jing He, Ting Wang, Danyang Liu, Jun Yang, Yuanpei He, Shouliang Zhao, Yanqin Ju","doi":"10.15283/ijsc23205","DOIUrl":"10.15283/ijsc23205","url":null,"abstract":"Schwann cells (SCs), a type of glial cell in the peripheral nervous system, can serve as a source of mesenchymal stem cells (MSCs) to repair injured pulp. This study aimed to investigate the role of SCs in tooth germ development and repair of pulp injury. We performed RNA-seq and immunofluorescent staining on tooth germs at different developmental stages. The effect of L-type calcium channel (LTCC) blocker nimodipine on SCs odontogenic differentiation was analyzed by real-time polymerase chain reaction and Alizarin Red S staining. We used the PLP1-CreERT2/ Rosa26-GFP tracing mice model to examine the role of SCs and Cav1.2 in self-repair after pulp injury. SC-specific markers expressed in rat tooth germs at different developmental stages. Nimodipine treatment enhanced mRNA levels of osteogenic markers (DSPP, DMP1, and Runx2) but decreased calcium nodule formation. SCs-derived cells increased following pulp injury and Cav1.2 showed a similar response pattern as SCs. The different SCs phenotypes are coordinated in the whole process to ensure tooth development. Blocking the LTCC with nimodipine promoted SCs odontogenic differentiation. Moreover, SCs participate in the process of injured dental pulp repair as a source of MSCs, and Cav1.2 may regulate this process.","PeriodicalId":14392,"journal":{"name":"International journal of stem cells","volume":" ","pages":"437-448"},"PeriodicalIF":2.5,"publicationDate":"2024-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11612224/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141787988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Generation of an Isogenic Hereditary Hemorrhagic Telangiectasia Model via Prime Editing in Human Induced Pluripotent Stem Cells. 通过在人类诱导多能干细胞中进行主基因编辑，生成异源遗传性出血性远端血管扩张症模型。

IF 2.5 4区医学

International journal of stem cells Pub Date : 2024-11-30 Epub Date: 2024-09-06 DOI: 10.15283/ijsc24084

Min Woo Kim, Kyu Sik Jeong, Jin Kim, Seul-Gi Lee, C-Yoon Kim, Hyung Min Chung

{"title":"Generation of an Isogenic Hereditary Hemorrhagic Telangiectasia Model via Prime Editing in Human Induced Pluripotent Stem Cells.","authors":"Min Woo Kim, Kyu Sik Jeong, Jin Kim, Seul-Gi Lee, C-Yoon Kim, Hyung Min Chung","doi":"10.15283/ijsc24084","DOIUrl":"10.15283/ijsc24084","url":null,"abstract":"Prime editing (PE) is a recently developed genome-editing technique that enables versatile editing. Despite its flexibility and potential, applying PE in human induced pluripotent stem cells (hiPSCs) has not been extensively addressed. Genetic disease models using patient-derived hiPSCs have been used to study mechanisms and drug efficacy. However, genetic differences between patient and control cells have been attributed to the inaccuracy of the disease model, highlighting the significance of isogenic hiPSC models. Hereditary hemorrhagic telangiectasia 1 (HHT1) is a genetic disorder caused by an autosomal dominant mutation in endoglin (ENG). Although previous HHT models using mice and HUVEC have been used, these models did not sufficiently elucidate the relationship between the genotype and disease phenotype in HHT, demanding more clinically relevant models that reflect human genetics. Therefore, in this study, we used PE to propose a method for establishing an isogenic hiPSC line. Clinically reported target mutation in ENG was selected, and a strategy for PE was designed. After cloning the engineered PE guide RNA, hiPSCs were nucleofected along with PEmax and hMLH1dn plasmids. As a result, hiPSC clones with the intended mutation were obtained, which showed no changes in pluripotency or genetic integrity. Furthermore, introducing the ENG mutation increased the expression of proangiogenic markers during endothelial organoid differentiation. Consequently, our results suggest the potential of PE as a toolkit for establishing isogenic lines, enabling disease modeling based on hiPSC-derived disease-related cells or organoids. This approach is expected to stimulate mechanistic and therapeutic studies on genetic diseases.","PeriodicalId":14392,"journal":{"name":"International journal of stem cells","volume":" ","pages":"397-406"},"PeriodicalIF":2.5,"publicationDate":"2024-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11612218/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142140084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Usp7 Regulates Glial Lineage Cell-Specific Transcription Factors by Modulating Histone H2B Monoubiquitination. Usp7 通过调节组蛋白 H2B 单泛素化来调控神经胶质系细胞特异性转录因子

IF 2.5 4区医学

International journal of stem cells Pub Date : 2024-11-30 Epub Date: 2024-07-02 DOI: 10.15283/ijsc23202

Dong-Ho Kim, Sammy L Kim, Vijai Singh, Suresh Ramakrishna

{"title":"Usp7 Regulates Glial Lineage Cell-Specific Transcription Factors by Modulating Histone H2B Monoubiquitination.","authors":"Dong-Ho Kim, Sammy L Kim, Vijai Singh, Suresh Ramakrishna","doi":"10.15283/ijsc23202","DOIUrl":"10.15283/ijsc23202","url":null,"abstract":"Histone H2B monoubiquitination (H2Bub1) is a dynamic posttranslational modification which are linked to DNA damage and plays a key role in a wide variety of regulatory transcriptional programs. Cancer cells exhibit a variety of epigenetic changes, particularly any aberrant H2Bub1 has frequently been associated with the development of tumors. Nevertheless, our understanding of the mechanisms governing the histone H2B deubiquitination and their associated functions during stem cell differentiation remain only partially understood. In this study, we wished to investigate the role of deubiquitinating enzymes (DUBs) on H2Bub1 regulation during stem cell differentiation. In a search for potential DUBs for H2B monoubiquitination, we identified Usp7, a ubiquitin-specific protease that acts as a negative regulator of H2B ubiquitination during the neuronal differentiation of mouse embryonic carcinoma cells. Loss of function of the Usp7 gene by a CRISPR/Cas9 system during retinoic acid-mediated cell differentiation contributes to the increase in H2Bub1. Furthermore, knockout of the Usp7 gene particularly elevated the expression of neuronal differentiation related genes including astryocyte-specific markers and oligodendrocyte-specific markers. In particular, glial lineage cell-specific transcription factors including oligodendrocyte transcription factor 2, glial fibrillary acidic protein, and SRY-box transcription factor 10 was significantly upregulated during neuronal differentiation. Thus, our findings suggest a novel role of Usp7 in gliogenesis in mouse embryonic carcinoma cells.","PeriodicalId":14392,"journal":{"name":"International journal of stem cells","volume":" ","pages":"427-436"},"PeriodicalIF":2.5,"publicationDate":"2024-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11612222/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141476599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exosomes Reshape the Osteoarthritic Defect: Emerging Potential in Regenerative Medicine-A Review. 外泌体重塑骨关节炎缺陷：再生医学的新兴潜力--综述。

IF 2.5 4区医学

International journal of stem cells Pub Date : 2024-11-30 Epub Date: 2024-01-22 DOI: 10.15283/ijsc23108

Jaishree Sankaranarayanan, Seok Cheol Lee, Hyung Keun Kim, Ju Yeon Kang, Sree Samanvitha Kuppa, Jong Keun Seon

{"title":"Exosomes Reshape the Osteoarthritic Defect: Emerging Potential in Regenerative Medicine-A Review.","authors":"Jaishree Sankaranarayanan, Seok Cheol Lee, Hyung Keun Kim, Ju Yeon Kang, Sree Samanvitha Kuppa, Jong Keun Seon","doi":"10.15283/ijsc23108","DOIUrl":"10.15283/ijsc23108","url":null,"abstract":"Osteoarthritis (OA) is a joint disorder caused by wear and tear of the cartilage that cushions the joints. It is a progressive condition that can cause significant pain and disability. Currently, there is no cure for OA, though there are treatments available to manage symptoms and slow the progression of the disease. A chondral defect is a common and devastating lesion that is challenging to treat due to its avascular and aneural nature. However, there are conventional therapies available, ranging from microfracture to cell-based therapy. Anyhow, its efficiency in cartilage defects is limited due to unclear cell viability. Exosomes have emerged as a potent therapeutic tool for chondral defects because they are a complicated complex containing cargo of proteins, DNA, and RNA as well as the ability to target cells due to their phospholipidic composition and the altering exosomal contents that boost regeneration potential. Exosomes are used in a variety of applications, including tissue healing and anti-inflammatory therapy. As in recent years, biomaterials-based bio fabrication has gained popularity among the many printable polymer-based hydrogels, tissue-specific decellularized extracellular matrix might boost the effects rather than an extracellular matrix imitating environment, a short note has been discussed. Exosomes are believed to be the greatest alternative option for current cell-based therapy, and future progress in exosome-based therapy could have a greater influence in the field of orthopaedics. The review focuses extensively on the insights of exosome use and scientific breakthroughs centered OA.","PeriodicalId":14392,"journal":{"name":"International journal of stem cells","volume":" ","pages":"381-396"},"PeriodicalIF":2.5,"publicationDate":"2024-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11612219/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139512542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0