International journal of stem cells最新文献_第2页

Regulatory Landscapes of Muscle Satellite Cells: From Mechanism to Application. 肌肉卫星细胞的调控景观：从机制到应用。

IF 2.4 4区医学

International journal of stem cells Pub Date : 2025-08-30 Epub Date: 2025-06-12 DOI: 10.15283/ijsc25037

Jeong Eun Lee, Sang Hoon Yoon, Kwan Seob Shim, Jeong Tae Do

引用次数: 0

Anti-Obesity Effects of Pear Extract (Pyrus pyrifolia): Clinical Implications and Therapeutic Potential. 梨提取物（Pyrus pyrifolia）的抗肥胖作用：临床意义和治疗潜力

IF 2.4 4区医学

International journal of stem cells Pub Date : 2025-08-14 DOI: 10.15283/ijsc25013

Yong Kyun Roh, Yoon-Su Ha, Taek-Kyong Kim, Kyungseok Kim, Soo Ro Kim, Da Hee Kim, Seonghwan Hwang, Se-Ran Yang, Sunbok Jang, Seung-Jin Kim

{"title":"Anti-Obesity Effects of Pear Extract (Pyrus pyrifolia): Clinical Implications and Therapeutic Potential.","authors":"Yong Kyun Roh, Yoon-Su Ha, Taek-Kyong Kim, Kyungseok Kim, Soo Ro Kim, Da Hee Kim, Seonghwan Hwang, Se-Ran Yang, Sunbok Jang, Seung-Jin Kim","doi":"10.15283/ijsc25013","DOIUrl":"https://doi.org/10.15283/ijsc25013","url":null,"abstract":"Mitigating obesity is a pivotal strategy for addressing metabolic diseases, including cardiovascular diseases, liver diseases, and diabetes. Although pear extract exhibits various pharmacological effects in regenerative diseases, such as skin, nerve, and adipose tissue dysfunction, by eliminating excessive reactive oxygen species, its exact clinical role in obesity has not been well studied. In this study, we investigated the efficacy and safety of pear extract (UPhenon®), a dietary ingredient extracted from unripe pear (Pyrus pyrifolia), in Korean participants with obesity. The participants underwent a 12-week, randomized, double-blind placebo-controlled trial. Body fat mass, body weight, body mass index (BMI), and hematological and blood chemistry parameters were assessed. No adverse effects or significant changes were observed in the supplementation or placebo groups. Additionally, the two groups did not differ significantly in body fat mass, body weight, BMI, hematological parameters, or blood chemistry parameters, except for the liver-specific enzyme, aspartate aminotransferase. Interestingly, compared with the supplementation and placebo groups, after 12 weeks of pear extract administration, a significant decrease in aspartate aminotransferase levels was recorded, indicating that pear extract partially attenuates obesity-induced liver damage. Furthermore, when compared with baseline measurements, a significant reduction in body fat mass (-802.61±1,460.82 g, p<0.0007), body weight (-1.28±1.29 kg, p<0.0001), and BMI (-0.46±0.46 kg/m2, p<0.0001) was observed after 12 weeks of pear extract administration. Taken together, our results suggest that pear extract supplementation may effectively reduce obesity without adverse effects and it may be a promising alternative to synthetic anti-obesity drugs.","PeriodicalId":14392,"journal":{"name":"International journal of stem cells","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144846566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Induced Pluripotent Stem Cells derived CD71^＋CD235a^＋ Erythroblasts Were Increased by Sirtuin 1 Activator. Sirtuin 1激活剂诱导CD71+CD235a+红母细胞增殖。

IF 2.4 4区医学

International journal of stem cells Pub Date : 2025-08-05 DOI: 10.15283/ijsc25040

Changyeong Kim, Kyung Hwan Park, Soo-Been Jeon, A-Reum Han, Ji Yoon Lee, Young-Sup Yoon

{"title":"Induced Pluripotent Stem Cells derived CD71＋CD235a＋ Erythroblasts Were Increased by Sirtuin 1 Activator.","authors":"Changyeong Kim, Kyung Hwan Park, Soo-Been Jeon, A-Reum Han, Ji Yoon Lee, Young-Sup Yoon","doi":"10.15283/ijsc25040","DOIUrl":"https://doi.org/10.15283/ijsc25040","url":null,"abstract":"Induced pluripotent stem cells (iPSCs) are a promising cell source for regenerative medicine. Clinical applications require a large number of functional red blood cells (RBCs), making it essential to ensure the proliferation of actively dividing, nucleated erythroblasts derived from iPSCs. Small molecules can enhance the efficiency and frequency of iPSC-derived cell differentiation. Sirtuin 1, a key enzyme in multiple biological processes, has been implicated in enhancing iPSC-derived cell differentiation. However, the specific effects of Sirtuin 1 on erythroblast proliferation from iPSCs remain unclear. Here, we developed a protocol to examine the effects of Sirtuin 1 on erythroblasts after endothelial-to-hematopoietic transition (EHT). We found that Sirtuin 1 activation increased the frequency of CD71＋CD235a＋ erythroblasts at the early stage after EHT, suggesting a role for Sirtuin 1 in the proliferation of these specified erythroblasts. These findings reveal that Sirtuin 1 activation benefits erythroblast proliferation and could be considered for translational application in large-scale RBC culture.","PeriodicalId":14392,"journal":{"name":"International journal of stem cells","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144784302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Kap1 Regulates Protein Stability of Nanog by Interfering with Fbxw8-Dependent Ubiquitination. Kap1通过干扰fbxw8依赖性泛素化调控Nanog蛋白稳定性。

IF 2.4 4区医学

International journal of stem cells Pub Date : 2025-08-05 DOI: 10.15283/ijsc25023

Hye Ji Moon, Nayeon Lee, Bo Seok Lee, Min Seok Park, Yoon Ji Jung, Ye Seul Kim, Jae Ho Kim

{"title":"Kap1 Regulates Protein Stability of Nanog by Interfering with Fbxw8-Dependent Ubiquitination.","authors":"Hye Ji Moon, Nayeon Lee, Bo Seok Lee, Min Seok Park, Yoon Ji Jung, Ye Seul Kim, Jae Ho Kim","doi":"10.15283/ijsc25023","DOIUrl":"https://doi.org/10.15283/ijsc25023","url":null,"abstract":"Nanog is a key transcription factor that regulates the self-renewal and pluripotency of embryonic stem cells (ESCs). Although Kap1 has been demonstrated to regulate the stability of stemness factors, including Oct4 and Lin28A, its role in regulating Nanog protein stability in ESCs remains unexplored. In the present study, we examined the interaction between Kap1 and Nanog and its role in stabilizing the Nanog protein. Immunoprecipitation assays revealed that Nanog specifically interacted with the coiled-coil domain of Kap1. Kap1 overexpression increased the stability of the Nanog protein by inhibiting its ubiquitination and proteasomal degradation, whereas Kap1 silencing accelerated Nanog degradation. Furthermore, Kap1 overexpression inhibits Nanog degradation by interfering with the binding of Nanog to Fbxw8, an E3 ubiquitin ligase that promotes Nanog degradation via a proteasome-dependent process. These results indicate that Kap1 acts as a key regulator to preserve ESC properties by modulating the protein stability of stemness factors, including Oct4, Lin28A, and Nanog.","PeriodicalId":14392,"journal":{"name":"International journal of stem cells","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144784303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent Applications of Lung Organoid and Lung-on-a-Chip Technologies for Evaluating the Toxicity of Fine Particulate Matter. 肺类器官和肺芯片技术在细颗粒物毒性评估中的最新应用。

IF 2.4 4区医学

International journal of stem cells Pub Date : 2025-07-30 DOI: 10.15283/ijsc25036

Minje Kang, Ji-Young Kim, Chan Woo Kwon, Woo Jin Kim, Seok-Ho Hong

{"title":"Recent Applications of Lung Organoid and Lung-on-a-Chip Technologies for Evaluating the Toxicity of Fine Particulate Matter.","authors":"Minje Kang, Ji-Young Kim, Chan Woo Kwon, Woo Jin Kim, Seok-Ho Hong","doi":"10.15283/ijsc25036","DOIUrl":"https://doi.org/10.15283/ijsc25036","url":null,"abstract":"There is mounting epidemiologic and experimental evidence of the harmful effects of exposure to fine particulate matter (PM2.5) on human lung health. However, the current utilization of in vitro two-dimensional (2D) cell culture and in vivo animal models falls short in accurately recapitulating the complexity, functions, and development of the human lung. Recently, technologies for creating 3D biomimetic lung organoids from human tissue and pluripotent stem cells that mimic the structure and function of human organs have been rapidly developed. Human lung organoids are currently being applied in various studies such as disease modeling, drug efficacy testing, and regenerative medicine. In the field of environmental toxicology, human lung organoids offer a promising solution for more accurately assessing the health impacts of exposure to PM2.5 and to the limitations posed by 2D cultures and animal models. In this review, we summarize the effects of PM2.5 exposure on human lung and other organoids, as well as the molecular and genetic impacts of such exposure using human organoids. Furthermore, the development of lung-on-a-chip technology to mimic the microenvironment and its utilization for evaluating the pulmotoxicity of PM2.5 exposure are discussed.","PeriodicalId":14392,"journal":{"name":"International journal of stem cells","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144742136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proteomic Profiling of Exosomes Derived from Endometrial Stem Cells and Adipose-Derived Stem Cells. 子宫内膜干细胞和脂肪干细胞外泌体的蛋白质组学分析。

IF 2.5 4区医学

International journal of stem cells Pub Date : 2025-06-18 DOI: 10.15283/ijsc25031

Jungwon Park, Jeongmin Lee, Yeon-Suk Kim, Yohan Oh

{"title":"Proteomic Profiling of Exosomes Derived from Endometrial Stem Cells and Adipose-Derived Stem Cells.","authors":"Jungwon Park, Jeongmin Lee, Yeon-Suk Kim, Yohan Oh","doi":"10.15283/ijsc25031","DOIUrl":"https://doi.org/10.15283/ijsc25031","url":null,"abstract":"Endometrial stem cells (EnSCs) are mesenchymal stem cells (MSCs) derived from endometrial tissue and serve as a valuable MSC source, as they are naturally replenished during menstruation. Exosomes, vesicles secreted by cells, contain various biomolecules such as proteins and nucleic acids and play crucial roles in intracellular communication, protein and nucleic acid metabolism, immune response regulation, and antigen presentation. This study investigated the protein profiles of EnSC-derived exosomes isolated from the endometrium of menstruating women and compared them with those of adipose-derived stem cell (ASC)-derived exosomes. After isolating EnSCs and ASCs, MSC characteristics were confirmed, and the purified exosomes were analyzed to determine their individual protein compositions. EnSCs, which can be obtained through non-invasive methods, exhibit multipotency similar to other MSCs and demonstrate rapid proliferation in vitro. Proteomic analysis of exosomal proteins revealed that 236 proteins were significantly more abundant in EnSC-derived exosomes than in ASC-derived exosomes, whereas 84 proteins were significantly more abundant in ASC-derived exosomes than in EnSC-derived exosomes. These findings indicate that EnSC-derived exosomes contain unique proteins compared to ASC-derived exosomes, as demonstrated through proteomic profiling. While further clinical studies are required, EnSCs hold promise as a potential therapeutic option in regenerative medicine, similar to current cell therapy products under development.","PeriodicalId":14392,"journal":{"name":"International journal of stem cells","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144316920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Temporal Regulation of Cytokines and Growth Factors for Optimized Hematopoietic-Lineage Specification from Human Pluripotent Stem Cells. 细胞因子和生长因子对人多能干细胞造血谱系优化的时间调控。

IF 2.5 4区医学

International journal of stem cells Pub Date : 2025-06-09 DOI: 10.15283/ijsc25020

Jisoo Park, Hyebin Koh, Eunsu Jeon, Kyungjun Uh, Sangjune Kim, Jong-Hee Lee

{"title":"Temporal Regulation of Cytokines and Growth Factors for Optimized Hematopoietic-Lineage Specification from Human Pluripotent Stem Cells.","authors":"Jisoo Park, Hyebin Koh, Eunsu Jeon, Kyungjun Uh, Sangjune Kim, Jong-Hee Lee","doi":"10.15283/ijsc25020","DOIUrl":"https://doi.org/10.15283/ijsc25020","url":null,"abstract":"Human pluripotent stem cells (hPSCs) can be used to investigate hematopoietic development and have the potential to advance cell-based therapies and to facilitate developmental biology studies. However, efficient ex vivo differentiation into hematopoietic lineages, including red blood cells (RBCs) of the erythroid lineage and immune cells such as macrophages of the myeloid lineage, is hampered by the need for precise temporal regulation of cytokines and growth factors. In this study, we developed an optimized protocol for hematopoietic lineage specification from hPSCs by fine-tuning the temporal dynamics of cytokine and growth factor applications. Prolonged mesodermal specification in the absence of hemogenic cytokines significantly enhanced the generation of hematopoietic progenitors (CD34＋CD45＋) with robust functional potential. Early administration of interleukin (IL)-3 during hematopoietic specification promoted progenitor expansion and maturation. Supplementation of bone morphogenetic protein 4 at the hematopoietic maturation stage enhanced the differentiation efficiency and preferentially drove myeloid lineage commitment toward macrophages at the expense of erythroid differentiation. The timing of erythropoietin administration was important in erythroid lineage commitment, and delayed treatment (day 10) enhanced erythroblast expansion and RBC production. By contrast, the timing of IL-6, GM-CSF, and M-CSF exposure did not significantly affect macrophage differentiation efficiency, suggesting that myeloid lineage specification follows a default pathway under optimized differentiation conditions. These findings suggest a refined, time-controlled strategy for directing hematopoietic differentiation from hPSCs, and provide insight into therapeutic blood cell production, regenerative medicine, and ex vivo modeling of hematopoietic disorders.","PeriodicalId":14392,"journal":{"name":"International journal of stem cells","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144247850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advanced Animal Replacement Testing Strategies Using Stem Cell and Organoids. 使用干细胞和类器官的先进动物替代试验策略。

IF 2.5 4区医学

International journal of stem cells Pub Date : 2025-05-30 Epub Date: 2025-03-11 DOI: 10.15283/ijsc24118

Chang-Jin Lee, Yoojun Nam, Yeri Alice Rim, Ji Hyeon Ju

{"title":"Advanced Animal Replacement Testing Strategies Using Stem Cell and Organoids.","authors":"Chang-Jin Lee, Yoojun Nam, Yeri Alice Rim, Ji Hyeon Ju","doi":"10.15283/ijsc24118","DOIUrl":"10.15283/ijsc24118","url":null,"abstract":"The increasing ethical concerns and regulatory restrictions surrounding animal testing have accelerated the development of advanced in vitro models that more accurately replicate human physiology. Among these, stem cell-based systems and organoids have emerged as revolutionary tools, providing ethical, scalable, and physiologically relevant alternatives. This review explores the key trends and driving factors behind the adoption of these models, such as technological advancements, the principles of the 3Rs (Replacement, Reduction, and Refinement), and growing regulatory support from agencies like the OECD and FDA. It also delves into the development and application of various model systems, including 3D reconstructed tissues, induced pluripotent stem cell-derived cells, and microphysiological systems, highlighting their potential to replace animal models in toxicity evaluation, disease modeling, and drug development. A critical aspect of implementing these models is ensuring robust quality control protocols to enhance reproducibility and standardization, which is necessary for gaining regulatory acceptance. Additionally, we discuss advanced strategies for assessing toxicity and efficacy, focusing on organ-specific evaluation methods and applications in diverse fields such as pharmaceuticals, cosmetics, and food safety. Despite existing challenges related to scalability, standardization, and regulatory alignment, these innovative models represent a transformative step towards reducing animal use and improving the relevance and reliability of preclinical testing outcomes.","PeriodicalId":14392,"journal":{"name":"International journal of stem cells","volume":" ","pages":"107-125"},"PeriodicalIF":2.5,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12122249/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143597061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Collagen Scaffold Augments the Therapeutic Effect of Human Umbilical Cord Mesenchymal Stem Cells in a Rat Model of Intrauterine Adhesion. 胶原支架增强人脐带间充质干细胞对大鼠宫内粘连模型的治疗作用。

IF 2.5 4区医学

International journal of stem cells Pub Date : 2025-05-30 Epub Date: 2025-02-25 DOI: 10.15283/ijsc24079

Linzhi Gao, Guifang Jiang, Enming Liang, Ying Zhang, Baoling Cheng, Xian Zhang, Dong Zhang, Xiaoyu Wang, Yuan Shen

{"title":"Collagen Scaffold Augments the Therapeutic Effect of Human Umbilical Cord Mesenchymal Stem Cells in a Rat Model of Intrauterine Adhesion.","authors":"Linzhi Gao, Guifang Jiang, Enming Liang, Ying Zhang, Baoling Cheng, Xian Zhang, Dong Zhang, Xiaoyu Wang, Yuan Shen","doi":"10.15283/ijsc24079","DOIUrl":"10.15283/ijsc24079","url":null,"abstract":"Intrauterine adhesion (IUA) caused by endometrial injury is the most common cause of female uterine infertility. Current treatments offer limited clinical benefits. In this study, we investigated the role of human umbilical cord mesenchymal stem cells (hUCMSCs) loaded collagen scaffold in the regeneration of injured human endometrium in an IUA rat model. Following the construction of the IUA rat model by mechanical injury, collagen scaffold, hUCMSCs, or hUCMSCs-loaded collagen scaffold was transplanted. The implantation of hUCMSCs-loaded collagen scaffold significantly increased the thickness of the endometrium, the number of endometrial glands and the abundance of blood vessels in IUA rats. Moreover, hUCMSCs-loaded collagen scaffold treatment significantly reduced endometrial fibrosis, increased the expression of Vegf, Integrin β3, Lif, and Igf-1, and finally improved endometrial receptivity in IUA rats. Taken together, our observations suggest that hUCMSCs-loaded collagen scaffold could be a practical therapeutic for treating IUA and restoring regeneration.","PeriodicalId":14392,"journal":{"name":"International journal of stem cells","volume":" ","pages":"135-145"},"PeriodicalIF":2.5,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12122250/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Dexamethasone and Tacrolimus on Mesenchymal Stem Cell Characteristics and Gene Expression. 地塞米松和他克莫司对间质干细胞特性和基因表达的影响

IF 2.5 4区医学

International journal of stem cells Pub Date : 2025-05-30 Epub Date: 2025-03-14 DOI: 10.15283/ijsc24116

Na Kyung Lee, Duk L Na, Su Hyeon Myeong, Seung-Yeon Lee, Na-Hee Lee, Hyemin Jang, Sang Won Seo, Jong Wook Chang, Hee Jin Kim, Hyo Jin Son

{"title":"Effects of Dexamethasone and Tacrolimus on Mesenchymal Stem Cell Characteristics and Gene Expression.","authors":"Na Kyung Lee, Duk L Na, Su Hyeon Myeong, Seung-Yeon Lee, Na-Hee Lee, Hyemin Jang, Sang Won Seo, Jong Wook Chang, Hee Jin Kim, Hyo Jin Son","doi":"10.15283/ijsc24116","DOIUrl":"10.15283/ijsc24116","url":null,"abstract":"Mesenchymal stem cells (MSCs) are frequently used for therapeutic applications in both pre-clinical and clinical settings owing to their capacity for immune modulation and neuroprotective effects. However, transient fever is commonly observed as an adverse event following MSC injection in patients with Alzheimer's disease (AD). In this study, we investigated the potential impact of immunosuppressants such as dexamethasone and tacrolimus on altering the characteristics of human mesenchymal stem cells (hMSCs). Additionally, we examined whether these immunosuppressants affect the persistence of hMSCs or the immune response upon their administration into the brain parenchyma of AD mice. The exposure of hMSCs to high concentrations of dexamethasone and tacrolimus in vitro did not significantly alter the characteristics of hMSCs. The expression of genes related to innate immune responses, such as Irak1, Irf3, Nod1, and Ifnar1, was significantly downregulated by the additional administration of dexamethasone and tacrolimus to the brain parenchyma of AD mice. However, hMSC persistence in the AD mouse brain was not affected. The results of this study support the use of immunosuppressants to mitigate fever during stem cell therapy in patients with AD.","PeriodicalId":14392,"journal":{"name":"International journal of stem cells","volume":" ","pages":"173-185"},"PeriodicalIF":2.5,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12122247/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143624738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0