International journal of stem cells最新文献_第3页

Anti-Obesity Effects of Pear Extract (Pyrus pyrifolia): Clinical Implications and Therapeutic Potential. 梨提取物（Pyrus pyrifolia）的抗肥胖作用：临床意义和治疗潜力

IF 2.6 4区医学

International journal of stem cells Pub Date : 2026-05-30 Epub Date: 2025-08-14 DOI: 10.15283/ijsc25013

Yong Kyun Roh, Yoon-Su Ha, Taek-Kyong Kim, Kyungseok Kim, Soo Ro Kim, Da Hee Kim, Seonghwan Hwang, Se-Ran Yang, Sunbok Jang, Seung-Jin Kim

{"title":"Anti-Obesity Effects of Pear Extract (Pyrus pyrifolia): Clinical Implications and Therapeutic Potential.","authors":"Yong Kyun Roh, Yoon-Su Ha, Taek-Kyong Kim, Kyungseok Kim, Soo Ro Kim, Da Hee Kim, Seonghwan Hwang, Se-Ran Yang, Sunbok Jang, Seung-Jin Kim","doi":"10.15283/ijsc25013","DOIUrl":"10.15283/ijsc25013","url":null,"abstract":"Mitigating obesity is a pivotal strategy for addressing metabolic diseases, including cardiovascular diseases, liver diseases, and diabetes. Although pear extract exhibits various pharmacological effects in regenerative diseases, such as skin, nerve, and adipose tissue dysfunction, by eliminating excessive reactive oxygen species, its exact clinical role in obesity has not been well studied. In this study, we investigated the efficacy and safety of pear extract (UPhenonⓇ), a dietary ingredient extracted from unripe pear (Pyrus pyrifolia), in Korean participants with obesity. The participants underwent a 12-week, randomized, double-blind placebo-controlled trial. Body fat mass, body weight, body mass index (BMI), and hematological and blood chemistry parameters were assessed. No adverse effects or significant changes were observed in the supplementation or placebo groups. Additionally, the two groups did not differ significantly in body fat mass, body weight, BMI, hematological parameters, or blood chemistry parameters, except for the liver-specific enzyme, aspartate aminotransferase. Interestingly, compared with the supplementation and placebo groups, after 12 weeks of pear extract administration, a significant decrease in aspartate aminotransferase levels was recorded, indicating that pear extract partially attenuates obesity-induced liver damage. Furthermore, when compared with baseline measurements, a significant reduction in body fat mass (-802.61±1,460.82 g, p<0.0007), body weight (-1.28±1.29 kg, p<0.0001), and BMI (-0.46±0.46 kg/m2, p<0.0001) was observed after 12 weeks of pear extract administration. Taken together, our results suggest that pear extract supplementation may effectively reduce obesity without adverse effects and it may be a promising alternative to synthetic anti-obesity drugs.","PeriodicalId":14392,"journal":{"name":"International journal of stem cells","volume":" ","pages":"242-255"},"PeriodicalIF":2.6,"publicationDate":"2026-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13218835/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144846566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

STOML2 Maintains Pluripotency and Cell Cycle Integrity in Human Pluripotent Stem Cells via PHB-ERK Signaling. STOML2通过PHB-ERK信号传导维持人类多能干细胞的多能性和细胞周期完整性。

IF 2.6 4区医学

International journal of stem cells Pub Date : 2026-05-30 Epub Date: 2026-02-19 DOI: 10.15283/ijsc25092

Yena Song, Bomi Kim, Ahyeon Kim, Dongyue No, Jeong Bin Lee, Hyun Kyu Kim, Jae Sang Oh, Sung-Hwan Moon, Dong-Yun Shin, Youngsok Choi, Man Ryul Lee

{"title":"STOML2 Maintains Pluripotency and Cell Cycle Integrity in Human Pluripotent Stem Cells via PHB-ERK Signaling.","authors":"Yena Song, Bomi Kim, Ahyeon Kim, Dongyue No, Jeong Bin Lee, Hyun Kyu Kim, Jae Sang Oh, Sung-Hwan Moon, Dong-Yun Shin, Youngsok Choi, Man Ryul Lee","doi":"10.15283/ijsc25092","DOIUrl":"10.15283/ijsc25092","url":null,"abstract":"Human pluripotent stem cells (hPSCs) can self-renew indefinitely and differentiate into all three germ layers. However, the primary regulators of hPSC cell cycle dynamics remain unclear. To identify novel regulators of hPSC proliferation, transcriptomic profiling of undifferentiated hPSCs and somatic cells was performed via next-generation sequencing. Stomatin-like protein 2 (STOML2) and prohibitin (PHB) were among the upregulated genes in hPSCs and were closely associated with the extracellular signal-regulated kinase/mitogen-activated protein kinase (ERK/MAPK) signaling pathway. Temporal expression analysis indicated that STOML2 and PHB decreased during differentiation and increased during reprogramming. Short hairpin RNA (shRNA)-mediated knockdown of STOML2 in hPSCs caused phenotypic changes. Gene expression analyses demonstrated reduced OCT4, NANOG, PHB, and phosphorylated ERK, alongside increased differentiation markers across all three germ layers. The STOML2-PHB axis is essential for maintaining hPSC identity by sustaining ERK/MAPK activity and cell cycle structure. This study identified STOML2 as a key pluripotency regulator and provides new insight into intrinsic stem cell fate control.","PeriodicalId":14392,"journal":{"name":"International journal of stem cells","volume":" ","pages":"157-171"},"PeriodicalIF":2.6,"publicationDate":"2026-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13218843/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146219937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PRPS1 (p.V42L) Mutation in Arts Syndrome Induces Aberrant Neural Stem Cell Development and Neuronal Senescence-Like Phenotype: Rescue by Nicotinamide Mononucleotide Supplementation. PRPS1 （p.V42L）突变诱导神经干细胞发育异常和神经衰老样表型：通过补充烟酰胺单核苷酸来拯救

IF 2.6 4区医学

International journal of stem cells Pub Date : 2026-05-30 Epub Date: 2026-03-06 DOI: 10.15283/ijsc25127

Ji-Hee Kim, Su-Jung Park, Jeong-A Lee, Chong Kun Cheon

{"title":"PRPS1 (p.V42L) Mutation in Arts Syndrome Induces Aberrant Neural Stem Cell Development and Neuronal Senescence-Like Phenotype: Rescue by Nicotinamide Mononucleotide Supplementation.","authors":"Ji-Hee Kim, Su-Jung Park, Jeong-A Lee, Chong Kun Cheon","doi":"10.15283/ijsc25127","DOIUrl":"10.15283/ijsc25127","url":null,"abstract":"Arts syndrome is a rare X-linked recessive neurodevelopmental disorder arising from pathogenic variants in PRPS1, which encodes phosphoribosyl pyrophosphate synthetase 1-an enzyme essential for de novo nucleotide biosynthesis. Affected individuals typically exhibit sensorineural hearing loss, intellectual disability, cerebellar ataxia, and recurrent infections. However, despite the severity of these clinical manifestations, therapeutic interventions remain limited, largely due to an incomplete understanding of the cellular pathophysiology underlying the disorder. In this study, we generated patient-specific induced pluripotent stem cells harboring the PRPS1 p.V42L variant and differentiated them into neural stem cells (NSCs) and neurons to elucidate disease mechanisms and explore potential therapeutic strategies. Patient-derived NSCs demonstrated significantly reduced proliferative capacity, aberrant nuclear morphology, and increased neuronal senescence, while mitochondrial integrity and function were largely preserved. Neurons differentiated from these NSCs exhibited impaired neurite outgrowth and reduced branching complexity, indicative of disrupted neurodevelopmental processes. Notably, supplementation with nicotinamide mononucleotide, a precursor of nicotinamide adenine dinucleotide (NAD＋), partially ameliorated defects in NSC proliferation, nuclear architecture, and neuronal morphology. Collectively, these findings delineate key cellular mechanisms underlying PRPS1-associated neurodevelopmental pathology and identify NAD＋ metabolic augmentation as a promising therapeutic avenue for Arts syndrome and related PRPS1-mediated disorders.","PeriodicalId":14392,"journal":{"name":"International journal of stem cells","volume":" ","pages":"186-196"},"PeriodicalIF":2.6,"publicationDate":"2026-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13218841/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147365346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stem Cell-Based Regeneration of Atrophied Vocal Folds after Recurrent Laryngeal Nerve Injury. 喉返神经损伤后萎缩声带的干细胞再生。

IF 2.6 4区医学

International journal of stem cells Pub Date : 2026-05-30 Epub Date: 2026-05-14 DOI: 10.15283/ijsc25106

Min Ji Lee, Se-Young Oh, Soo Yeon Jung, Ha Yeong Kim, So Jeong Kim, Han Su Kim

{"title":"Stem Cell-Based Regeneration of Atrophied Vocal Folds after Recurrent Laryngeal Nerve Injury.","authors":"Min Ji Lee, Se-Young Oh, Soo Yeon Jung, Ha Yeong Kim, So Jeong Kim, Han Su Kim","doi":"10.15283/ijsc25106","DOIUrl":"10.15283/ijsc25106","url":null,"abstract":"This study evaluated the therapeutic potential of calcium hydroxylapatite (CaHA) and tonsil-derived mesenchymal stem cells (TMSCs) suspended in hyaluronic acid (HA) for regenerating atrophied vocal fold in a rabbit model of recurrent laryngeal nerve (RLN) injury. Vocal fold atrophy was induced by transecting the unilateral RLN in rabbits. Histological changes in muscle fiber (H&E), cartilage (Safranin O), and collagen deposition (Masson's trichrome), were assessed at 8, 12, 16, and 26 weeks post-injury to confirm the establishment of vocal fold atrophy model. CaHA or TMSC＋HA was injected into the atrophied vocal folds. After 8 weeks, laryngeal tissues were harvested to evaluate tissue morphology and the engraftment of transplanted cells using histological staining (H&E, Safranin O, Masson's trichrome, Alizarin Red S) and immunofluorescence analysis for human mitochondria. At 8 weeks after RLN transection, CaHA injection-maintained medialization of the atrophied vocal fold but failed to promote tissue regeneration and induced localized foreign body reaction characterized by multinucleated giant cells and granular calcium deposit. In contrast, TMSC＋HA injection markedly enhanced muscle fiber regeneration, reduced interstitial spaces between muscle fibers, and did not form fibrous capsule. Moreover, TMSC＋HA did not elicit immune reactivity or tissue calcification, suggesting superior biocompatibility and tissue integration. These findings indicate that while CaHA acts as a durable filler regenerating potential, TMSC＋HA offers distinct advantages by promoting muscle regeneration, and extracellular matrix remodeling. TMSC＋HA hydrogel presents a promising regenerative strategy for restoring atrophied vocal folds following RLN injury, demonstrating excellent biocompatibility and effective tissue integration.","PeriodicalId":14392,"journal":{"name":"International journal of stem cells","volume":" ","pages":"197-211"},"PeriodicalIF":2.6,"publicationDate":"2026-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13222849/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147929668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigating the Causal Links between the Aging Process and Alzheimer's Disease Pathogenesis. 研究衰老过程与阿尔茨海默病发病机制的因果关系。

IF 2.6 4区医学

International journal of stem cells Pub Date : 2026-05-30 Epub Date: 2026-03-06 DOI: 10.15283/ijsc25100

Jaewoo Seok, Hyein Lee, Jinsoo Seo

引用次数: 0

Exploring the Potential of Wharton's Jelly-Derived Mesenchymal Stem Cells as a Therapeutic Approach for Preterm Premature Rupture of Membranes. 探索沃顿果冻源间充质干细胞作为早产儿胎膜早破治疗方法的潜力。

IF 2.6 4区医学

International journal of stem cells Pub Date : 2026-05-30 Epub Date: 2026-03-11 DOI: 10.15283/ijsc25048

Hyeongseop Kim, Hyunju Kim, Suyeon Jeon, Soo-Young Oh, Sang Eon Park, Hong Bae Jeon, Jung-Sun Kim, Suk-Joo Choi, Jong Wook Chang

{"title":"Exploring the Potential of Wharton's Jelly-Derived Mesenchymal Stem Cells as a Therapeutic Approach for Preterm Premature Rupture of Membranes.","authors":"Hyeongseop Kim, Hyunju Kim, Suyeon Jeon, Soo-Young Oh, Sang Eon Park, Hong Bae Jeon, Jung-Sun Kim, Suk-Joo Choi, Jong Wook Chang","doi":"10.15283/ijsc25048","DOIUrl":"10.15283/ijsc25048","url":null,"abstract":"Preterm premature rupture of membranes (PPROM) is a major cause of preterm birth and neonatal morbidity, with no established treatment to restore membrane integrity. Mesenchymal stem cells (MSCs), known for their regenerative and immunomodulatory properties, harbor promising therapeutic potential for fetal membranes repair. This study aimed to evaluate the rapid barrier reinforcement effect of Wharton's jelly-derived mesenchymal stem cells (WJ-MSCs) using in vitro and ex vivo models of PPROM. A wound healing assay was conducted to assess the effect of WJ-MSCs on human amniotic epithelial cells proliferation and migration. An ex vivo PPROM model was established using incised human fetal membranes to simulate membrane rupture. WJ-MSCs were directly applied to the lesion site at varying doses. Therapeutic efficacy was evaluated by a leak test and histological analysis. WJ-MSCs and their conditioned medium significantly enhanced epithelial wound closure in vitro, demonstrating that soluble paracrine factors secreted by WJ-MSCs have the potential to aid membrane injury restoration. In the ex vivo PPROM model, WJ-MSCs treatment reduced both leakage area and frequency of fetal membranes, with the most significant effect observed at a medium dose (5.0×105 cells). Histological evaluations revealed partial recovery of the fetal membranes, particularly the chorion layer. These findings suggest that WJ-MSCs contribute to rapid barrier reinforcement of injured fetal membranes. Taken together, WJ-MSCs enhance epithelial healing and support restoration of membrane integrity, highlighting their potential as a therapeutic approach for PPROM. Further, in vivo studies are required to confirm therapeutic efficacy and evaluate safety.","PeriodicalId":14392,"journal":{"name":"International journal of stem cells","volume":" ","pages":"212-223"},"PeriodicalIF":2.6,"publicationDate":"2026-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13218840/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147432899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neuroprotective Effects of Transplanted Induced Pluripotent Stem Cell-Derived Neural Precursors in Huntington's Disease Models. 移植诱导多能干细胞衍生的神经前体在亨廷顿病模型中的神经保护作用。

IF 2.6 4区医学

International journal of stem cells Pub Date : 2026-05-30 Epub Date: 2026-03-27 DOI: 10.15283/ijsc26001

Hyeonjoong Jeon, Il-Shin Lee, Dong Gyu Lee, Kyoung Seo Park, Sanghun Lee, Hyo Chang Park, Beomsoo Kim, Hyun Sook Kim, Jihwan Song

{"title":"Neuroprotective Effects of Transplanted Induced Pluripotent Stem Cell-Derived Neural Precursors in Huntington's Disease Models.","authors":"Hyeonjoong Jeon, Il-Shin Lee, Dong Gyu Lee, Kyoung Seo Park, Sanghun Lee, Hyo Chang Park, Beomsoo Kim, Hyun Sook Kim, Jihwan Song","doi":"10.15283/ijsc26001","DOIUrl":"10.15283/ijsc26001","url":null,"abstract":"Huntington's disease (HD) is characterized by progressive striatal degeneration associated with mutant huntingtin (mHTT)-related proteostatic disruption and chronic neuroinflammation. Although mHTT-lowering approaches hold therapeutic promise, their capacity to restore the degenerating neural microenvironment remains limited. Here, we evaluated the therapeutic potential of human induced pluripotent stem cell (iPSC)-derived neural precursor cells (s513-NPCs) in two complementary HD models, the acute R6/2 transgenic fragment model and the protracted, full-length YAC128 genomic model. Intrastriatal transplantation of s513-NPCs resulted in sustained functional improvement, including stabilization of motor coordination and attenuation of neuromuscular decline, across both disease contexts. These neuroprotective effects were accompanied by efficient donor cell engraftment and integration within the host striatum. At the molecular level, transplantation was associated with coordinated changes in proteostasis-related pathways, reflected by reduced mHTT aggregate burden and modulation of proteasomal and autophagic markers. In parallel, enhanced local BDNF-TrkB signaling was observed in grafted regions, consistent with improved neuronal support. Notably, transplanted NPCs exhibited context-dependent immunological responses, characterized by attenuation of pro-inflammatory signatures in aggressive disease stages and features of a reparative microenvironment in more protracted settings. Collectively, these findings demonstrate that iPSC-derived neural precursor transplantation confers robust neuroprotective effects in HD models, supporting its potential as a stem cell-based strategy to mitigate striatal pathology and functional decline.","PeriodicalId":14392,"journal":{"name":"International journal of stem cells","volume":" ","pages":"172-185"},"PeriodicalIF":2.6,"publicationDate":"2026-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13218842/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147520756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multiscale Convergence of Signaling, Epigenetic, and Environmental Networks in Mammalian Palatogenesis and Cleft Palate Pathogenesis. 哺乳动物腭裂发病过程中信号、表观遗传和环境网络的多尺度趋同。

IF 2.6 4区医学

International journal of stem cells Pub Date : 2026-05-30 Epub Date: 2026-05-18 DOI: 10.15283/ijsc26020

Hyung-Jin Won, Beomjong Song, Yujeong Song, Jeong-Oh Shin

{"title":"Multiscale Convergence of Signaling, Epigenetic, and Environmental Networks in Mammalian Palatogenesis and Cleft Palate Pathogenesis.","authors":"Hyung-Jin Won, Beomjong Song, Yujeong Song, Jeong-Oh Shin","doi":"10.15283/ijsc26020","DOIUrl":"10.15283/ijsc26020","url":null,"abstract":"Palatogenesis is tightly regulated at cellular and molecular levels. Cranial neural crest cells (CNCCs), multipotent stem cells that generate most palatal mesenchyme, self-renew, decide fate, and differentiate under cues from their stem cell niche. Orofacial clefts (OFC), among the most common congenital birth defects, arise when CNCC-mediated palate formation is disturbed. Here we review genes, epigenetic machineries, and environmental insults regulating secondary palate development, focusing on CNCC stem cell biology and translational perspectives in tissue engineering, gene therapy, and regenerative medicine for OFC. Canonical signaling pathways regulating palatal shelf proliferation, growth, elevation, and fusion, including Tgf-beta superfamily and Bmp, Fgf, Wnt, and Shh, function as morphogens directing tissue morphogenesis and as regulators of the progenitor niche derived from neural crest cells, balancing proliferation and differentiation. We also overview debated topics such as signaling crosstalk and dissolution of MES. DNA methylation, histone marks, and noncoding RNAs comprise the epigenetic machinery preserving CNCC identity and plasticity. Environmental exposures including smoking, alcohol consumption, and folate deficiency disrupt genetic and epigenetic programs, perturb normal palate development, and elevate OFC risk. Finally, we discuss emerging technologies including single-cell multi-omics, organoids, and CRISPR-based epigenome editing. We propose an integrative conceptual model that places CNCC state transitions at the center of a complex regulatory network. This model emphasizes that disruption at any level, whether arising from niche interactions, epigenetic programming, or environmental exposures, can precipitate cleft palate pathogenesis.","PeriodicalId":14392,"journal":{"name":"International journal of stem cells","volume":" ","pages":"129-145"},"PeriodicalIF":2.6,"publicationDate":"2026-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13218834/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147963653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Altered Properties of Bone Marrow-Derived Mesenchymal Stem Cells Exposed to Serum from Patients with Acute-On-Chronic Liver Failure. 急性-慢性肝衰竭患者血清中骨髓间充质干细胞特性的改变

IF 2.6 4区医学

International journal of stem cells Pub Date : 2026-05-30 DOI: 10.15283/ijsc25085

Wenxin Zhang, Shurong Liu, Zhiru Wang, Chang Sun, Baikun Liu, Lei Zhang

{"title":"Altered Properties of Bone Marrow-Derived Mesenchymal Stem Cells Exposed to Serum from Patients with Acute-On-Chronic Liver Failure.","authors":"Wenxin Zhang, Shurong Liu, Zhiru Wang, Chang Sun, Baikun Liu, Lei Zhang","doi":"10.15283/ijsc25085","DOIUrl":"10.15283/ijsc25085","url":null,"abstract":"Acute-on-chronic liver failure (ACLF) is a high-mortality syndrome characterized by acute decompensation of liver function. Oxidative stress exerts a pivotal regulatory role in ACLF. Mesenchymal stem cell (MSC)-based therapy is a promising strategy for ACLF, with well-documented safety and efficacy profiles. However, long-term exposure to an oxidative microenvironment can impair MSC regenerative capacity and trigger senescence. Thus, elucidating how the pathological ACLF serum microenvironment affects the biological characteristics of MSCs is important for advancing MSC-based therapeutic strategies. This study investigated the biological alterations of MSCs exposed to ACLF patient serum. We demonstrated that ACLF serum markedly impaired MSC morphology, proliferation, and migration, induced energy metabolism dysfunction, and promoted senescence in vitro. In addition, the levels of advanced oxidation protein products (AOPPs), which are oxidative stress markers, were significantly elevated in the serum of ACLF patients, and this increase was positively correlated with the extent of MSCs senescence. Furthermore, we discovered that ACLF patient serum could lead to elevated intracellular reactive oxygen species (ROS) levels, which in turn drove MSCs senescence. Taken together, these findings suggested that the oxidative microenvironment of ACLF patient serum could alter the functional properties of MSCs and accelerate MSCs senescence through the induction of excessive ROS production. These results help clarify the pivotal role of AOPPs in mediating MSCs senescence and functional impairment, and highlight that monitoring serum AOPPs levels may be a promising biomarker for determining the optimal time window for MSCs transplantation in patients with ACLF.","PeriodicalId":14392,"journal":{"name":"International journal of stem cells","volume":"19 2","pages":"224-241"},"PeriodicalIF":2.6,"publicationDate":"2026-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13218836/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148054560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated Electrophysiological and Optical Analysis of Human iPSC-Derived Cardiomyocytes Using Transparent ITO Microelectrode Chip. 利用透明ITO微电极芯片对人ipsc衍生心肌细胞进行电生理和光学综合分析。

IF 2.6 4区医学

International journal of stem cells Pub Date : 2026-05-30 Epub Date: 2026-02-03 DOI: 10.15283/ijsc25077

Seul-Gi Lee, Shinhye Park, C-Yoon Kim, Hyung Min Chung

{"title":"Integrated Electrophysiological and Optical Analysis of Human iPSC-Derived Cardiomyocytes Using Transparent ITO Microelectrode Chip.","authors":"Seul-Gi Lee, Shinhye Park, C-Yoon Kim, Hyung Min Chung","doi":"10.15283/ijsc25077","DOIUrl":"10.15283/ijsc25077","url":null,"abstract":"Cardiotoxicity assessment is a crucial step in the drug development process. With growing interest in alternatives to animal testing, preclinical cardiotoxicity evaluation has become increasingly important. Human-induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) offer a physiologically relevant in vitro model for this purpose. As a result, electrophysiological analysis platforms using iPSC-CMs have gained attention. However, conventional microelectrode array (MEA) chips rely on metal electrodes, which are costly and optically opaque. This lack of transparency limits detailed morphological observation of the cells. In this study, we employed an MEA chip incorporating transparent and conductive indium tin oxide (ITO) electrodes to simultaneously monitor both morphological changes and field potential (FP) of iPSC-CMs. iPSC-CMs cultured on ITO chips exhibited stable electrophysiological signals reflecting coupled depolarization and repolarization, along with self-organization. Short-term exposure to ion channel blockers did not induce noticeable morphological alterations; however, dose-dependent changes in FPs were observed. In contrast, treatment with cardiotoxic drugs resulted in morphological damage and reduced cell viability, accompanied by progressive alterations in key FP parameters over the treatment period. These findings demonstrate the potential of ITO-based MEA as next-generation cardiotoxicity evaluation platforms capable of real-time monitoring of both drug-induced electrophysiological responses and optical cellular changes.","PeriodicalId":14392,"journal":{"name":"International journal of stem cells","volume":" ","pages":"146-156"},"PeriodicalIF":2.6,"publicationDate":"2026-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13218844/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146105438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0