An Efficient Endothelial Cell Differentiation Protocol Using Bioactive Lipid O-Cyclic Phytosphingosine-1-Phosphate in Human Embryonic Stem Cells.

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS

ACS Applied Bio Materials Pub Date : 2024-09-10 DOI:10.15283/ijsc24068

Ki-Sang Jo,Won-Jun Jo,Ainsley Mike Antao,Janardhan Keshav Karapurkar,Young Jun Park,Myeong-Jun Choi,Suresh Ramakrishna,Kye-Seong Kim

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Abstract

Bioactive lipids like sphingosine-1-phosphate (S1P) and lysophosphatidic acid have gained significant attention as signaling molecules with regulatory roles in stem cell proliferation and differentiation. The novel chemically synthesized sphingosine metabolite O-cyclic phytosphingosine-1-phosphate (cP1P) is derived from phytosphingosine-1-phosphate (P1P) and shares structural similarities with S1P. Previously, the role of cP1P in regulating ALK3/BMPR signaling during cardiomyocyte differentiation from human embryonic stem cells (hESCs) was demonstrated. In this study, the applicability of cP1P for endothelial cells (ECs) differentiation from hESCs was investigated an efficient method to obtain a high yield of functional ECs over several passages was standardized. The ECs derived from hESCs showed cellular and molecular characteristics similar to the native ECs. Thus, the results of this study open avenues for further research into cP1P-based stem cell differentiation for regenerative therapies.

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在人胚胎干细胞中使用生物活性脂质 O-Cyclic Phytosphingosine-1-Phosphate 的高效内皮细胞分化方案。

鞘氨醇-1-磷酸（S1P）和溶血磷脂酸等生物活性脂类作为在干细胞增殖和分化过程中起调控作用的信号分子，受到了广泛关注。化学合成的新型鞘氨醇代谢物 O-环植物鞘氨醇-1-磷酸（cP1P）来源于植物鞘氨醇-1-磷酸（P1P），与 S1P 结构相似。此前，研究证实了 cP1P 在人类胚胎干细胞（hESCs）心肌细胞分化过程中调节 ALK3/BMPR 信号传导的作用。本研究调查了cP1P在从hESCs分化内皮细胞（ECs）过程中的适用性。从 hESCs 中分化出的 ECs 显示出与原生 ECs 相似的细胞和分子特征。因此，这项研究结果为进一步研究基于cP1P的干细胞分化再生疗法开辟了道路。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ACS Applied Bio Materials Chemistry-Chemistry (all)

CiteScore

9.40

自引率

2.10%

发文量

464