International Journal of Reproductive Biomedicine最新文献

{"title":"IL-6-174 G/C transversion might decrease male infertility risk: A case-control study.","authors":"Tayyebeh Zamani-Badi, Mohammad Karimian, Javad Amini Mahabadi, Atieh Rafatmanesh, Hossein Nikzad","doi":"10.18502/ijrm.v22i12.18067","DOIUrl":"10.18502/ijrm.v22i12.18067","url":null,"abstract":"<p><strong>Background: </strong>Genetic predispositions have been identified as important factors in male infertility. Among the many genes related to male reproductive function, interleukin 6 (IL-6) has emerged as a key player. Despite the growing recognition of genetic factors in male infertility, the specific association between the IL-6-174 G/C genetic polymorphism and male infertility remains an area that needs further investigation.</p><p><strong>Objective: </strong>This investigation explores the correlation between the IL-6-174 G/C transversion and male infertility.</p><p><strong>Materials and methods: </strong>In a case-control study, a total of 314 men who referred to the Kashan Infertility Center, Shahid Beheshti hospital, Kashan, Iran, were enrolled for IL-6-174 G/C polymorphism analysis. The study comprised 163 infertile participants as the case group and 151 fertile men as the control group. Following the screening, 2 ml of whole blood was collected from each participant. Cases were categorized into 3 subgroups based on World Health Organization criteria: (i) nonobstructive azoospermia (n = 42), (ii) oligozoospermia (n = 61), and (iii) asthenozoospermia (n = 60). After DNA extraction, genotypes of the samples at the -174 G/C (rs1800795) locus were determined using the polymerase chain reaction-restriction fragment length polymorphism method.</p><p><strong>Results: </strong>Our genetic investigation demonstrated a significant association between the GC genotype and male infertility. Furthermore, a correlation was observed between the heterozygous GC genotype and reduced risk of oligozoospermia and asthenospermia. Additionally, the C allele was correlated with a decreased risk of infertility and specific subgroups such as oligozoospermia and asthenospermia.</p><p><strong>Conclusion: </strong>Our findings suggest that the IL-6-174 G/C transversion could potentially serve as a protective genetic factor against male infertility.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"22 12","pages":"1015-1024"},"PeriodicalIF":1.6,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11830927/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143449003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing psychological health and reproductive function: Depression, anxiety, and stress in infertile men compared to controls: A case-control study.","authors":"Seyedeh Narjes Roudbaraki, Maryam Ramezani, Bita Saifi, Mostafa Salimi, Massood Issapour Cheshani","doi":"10.18502/ijrm.v22i12.18068","DOIUrl":"10.18502/ijrm.v22i12.18068","url":null,"abstract":"<p><strong>Background: </strong>With the increasing prevalence of infertility and its links to depression, anxiety, and stress, it is essential to compare these mental health levels between infertile men and a control group.</p><p><strong>Objective: </strong>This study aimed to compare depression, anxiety, and stress among infertile and fertile men. Also, assessing demographic factors affecting these challenges among both groups.</p><p><strong>Materials and methods: </strong>This case-control study was conducted on 120 men at Milad Infertility Research Center, Mashhad, Iran from January 2023 to February 2023. Participants were divided into 2 groups: 60 infertile men and 60 healthy men who did not have fertility problems. Demographic information such as age, education, occupation, duration of the marriage, and duration of infertility was collected from their medical records, and they also completed the depression, anxiety, and stress scale 21 questionnaire through a telephonic interview. Finally, the findings were statistically analyzed.</p><p><strong>Results: </strong>Severe and very severe depression, anxiety, and stress were observed in 65%, 60%, and 43.4% of infertile men and 16.7%, 23.3%, and 11.7% of fertile men, respectively. Which was significantly more than the fertile group, and there was a significant relationship between depression (p <math><mo>≤</mo></math> 0.001), anxiety (p = 0.001), stress (p <math><mo>≤</mo></math> 0.001), and infertility. Also, a significant relationship was observed between the duration of infertility and depression (p = 0.031).</p><p><strong>Conclusion: </strong>Our study found infertile men had higher stress, anxiety, and depression than the control group. Limitations included phone-based data collection and the depression, anxiety, and stress scale 21 questionnaire's screening nature. Future studies should involve larger populations and consider economic status as a variable related to mental health.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"22 12","pages":"1025-1034"},"PeriodicalIF":1.6,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11830923/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143448973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Decreased expression of <i>miR-200a</i> and <i>miR-223-3p</i> in endometriosis during the secretory phase of menstrual cycle: Insights from a case-control study on molecular biomarkers and disease-related infertility.","authors":"Yasaman Nazari Hagh, Mohamadreza Ahmadifard, Sedigheh Esmaelzadeh, Soheila Abbaszadeh, Naser Shokrzadeh","doi":"10.18502/ijrm.v22i12.18066","DOIUrl":"10.18502/ijrm.v22i12.18066","url":null,"abstract":"<p><strong>Background: </strong>Endometriosis (EM) is a condition that causes infertility with decreasing uterine receptivity. It is reported that it affects about 20-25% of all infertile women. Some genetic markers play a crucial role in pathogenesis and infertility.</p><p><strong>Objective: </strong>This study investigates the role of <i>miR-200a</i> and <i>miR-223-3p</i> in embryo implantation and their association with EM-related infertility.</p><p><strong>Materials and methods: </strong>In this case-control study, 36 women who referred to the Center for Research on Reproductive Health and Infertility of Babol University of Medical Sciences and Fatemeh Al-Zahra Infertility Specialized Treatment Center in Babol, Iran between June 2022 and July 2023 were evaluated. Participants were divided into 2 EM and control groups (n = 18/each). Endometrial samples were collected from participants between 17 <math> <msup><mrow><mi> </mi></mrow> <mtext>th</mtext></msup> </math> and 24 <math> <msup><mrow><mi> </mi></mrow> <mtext>th</mtext></msup> </math> days of their menstrual cycle. Histopathological examination (hematoxylin and eosin and periodic acid schiff) was performed to confirm the secretory stage, and <i>miR-200a</i> and <i>miR-223-3p</i> expressions were analyzed by quantitative reverse transcriptase-polymerase chain reaction.</p><p><strong>Results: </strong>Histological analysis confirmed that both groups were in the secretory stage. Additionally, miRNA expression results showed a significant decrease in the <i>miR-200a</i> and <i>miR-223-3p</i> expression levels in EM group compared to control group. The expression level of <i>miR-223-3p</i> and <i>miR-200a</i> in the eutopic endometrial tissue of women with EM was notably lower than those in the control group.</p><p><strong>Conclusion: </strong>Our results suggest that <i>miR-200a</i> and <i>miR-223-3p</i> are involved in the EM pathogenesis, while other genes and signaling pathways are probably involved in the implantation failure.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"22 12","pages":"1003-1014"},"PeriodicalIF":1.6,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11830922/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143449001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Closer look at circulating nitric oxide levels and their association with polycystic ovary syndrome: A meta-analytical exploration.","authors":"Seyed Sobhan Bahreiny, Akram Ahangarpour, Elnaz Harooni, Mahdi Amraei, Mojtaba Aghaei, Reza Mohammadpour Fard","doi":"10.18502/ijrm.v22i12.18061","DOIUrl":"10.18502/ijrm.v22i12.18061","url":null,"abstract":"<p><strong>Background: </strong>Polycystic ovary syndrome (PCOS) casts a wide shadow over the reproductive health of millions of women worldwide, emerging as one of the most complex and multifaceted endocrine disorders. In addition, nitric oxide (NO) stands out as a pivotal signaling molecule, orchestrating a symphony of physiological processes.</p><p><strong>Objective: </strong>This meta-analysis aims to elucidate the association between NO levels and PCOS, investigate the potential of NO as a biomarker for PCOS diagnosis, and evaluate its clinical significance.</p><p><strong>Materials and methods: </strong>A systematic review was conducted in several electronic databases, including PubMed, Web of Science, Cochrane Library, Scopus, EMBASE, and Google Scholar, to identify relevant studies published up to January 2024. Standardized mean difference and 95% CI were calculated using a random effects model to assess the overall effect size. Meta-regressions and subgroup analysis were performed to investigate sources of heterogeneity.</p><p><strong>Results: </strong>A meta-analysis of 14 studies with 1171 participants showed that NO levels were significantly lower in the PCOS group than in the control group. The pooled analysis yielded a standardized mean difference of -0.482; 95% CI: -0.908 to -0.056; p = 0.027. Subgroup analyses further demonstrated variations in NO levels between different PCOS phenotypes and in relation to metabolic parameters.</p><p><strong>Conclusion: </strong>This meta-analysis provides evidence for an association between PCOS and dysregulated NO levels and suggests a potential role of NO as a biomarker in the diagnosis and pathogenesis of PCOS.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"22 12","pages":"943-962"},"PeriodicalIF":1.6,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11830925/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143448996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of salivary antioxidant level in polycystic ovary syndrome women under assisted reproductive technology treatment: A case-control study.","authors":"Narges Gholizadeh, Maryam Koopaie, Ashraf Aleyasin, Atousa Mortazavi Milani, Marziyeh Aghahosseini, Mohammad Javad Kharrazifard, Mohadeseh Bahmaee","doi":"10.18502/ijrm.v22i12.18063","DOIUrl":"10.18502/ijrm.v22i12.18063","url":null,"abstract":"<p><strong>Background: </strong>Infertility is defined as the inability to conceive after 12 months of regular unprotected intercourse. Approximately 85% of infertile couples have an identifiable cause, one of the most common causes of infertility is polycystic ovary syndrome (PCOS). The reduction of antioxidant levels in serum and follicular fluid in infertile women compared to healthy women shows the importance of further studying these markers.</p><p><strong>Objective: </strong>To study salivary and serum antioxidant levels in PCOS participants under assisted reproductive technology.</p><p><strong>Materials and methods: </strong>This case-control study was conducted on 80 women in 2 groups including normal participants as control and PCOS groups (n = 40/each). Serum and salivary antioxidant levels such as saliva superoxide dismutase (SOD), saliva anti-Müllerian hormone (AMH), serum SOD, serum total oxidant status, and serum AMH were measured.</p><p><strong>Results: </strong>The average age of participants was 31.6 <math><mo>±</mo></math> 5.4 yr. In both the saliva and serum, antioxidant levels differed significantly between the PCOS and control groups. Key findings showed that the PCOS group had different antioxidant levels and higher serum AMH levels compared to the control group, with all differences being statistically significant (p <math><mo><</mo></math> 0.05).</p><p><strong>Conclusion: </strong>Our finding underscored that saliva antioxidant levels, especially SOD, are a good marker for PCOS diagnosis. It is noninvasive, can easily be performed by the participants, and can be collected in various settings without specialized equipment.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"22 12","pages":"975-984"},"PeriodicalIF":1.6,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11830924/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143449021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of dienogest and gonadotropin-releasing hormone agonist on pelvic pain after laparoscopic surgery for endometriosis: An RCT.","authors":"Fatemeh Davari Tanha, Azam Rasti, Hamideh Pakniat, Shohreh Salimi Setudeh","doi":"10.18502/ijrm.v22i12.18065","DOIUrl":"10.18502/ijrm.v22i12.18065","url":null,"abstract":"<p><strong>Background: </strong>Endometriosis is a chronic inflammatory condition associated with debilitating chronic pelvic pain that affects women's quality of life. Several drugs have been used to reduce pain and psychological distress associated with this disease. Currently, gonadotropin-releasing hormone (GnRH) agonists and dienogest are the most widely used medical therapies for endometriosis.</p><p><strong>Objective: </strong>This study aimed to investigate the efficacy of dienogest and GnRH agonists in improving pelvic pain after laparoscopic surgery for endometriosis.</p><p><strong>Materials and methods: </strong>In this randomized clinical trial study, 104 women with endometriosis who were referred to the Department of Reproductive Medicine of Yas hospital, Tehran, Iran, between April 2022 and March 2023 were studied. After laparoscopic surgery, individuals were randomly assigned into 2 groups (n = 52/each): the dienogest-administered group and the GnRH agonist-administered group. Participants were followed up at 3 months and pelvic pain was measured using the visual analog scale. Pelvic pain and adverse effects of drugs were compared between the groups.</p><p><strong>Results: </strong>Pelvic pain significantly improved in both treatment groups (p <math><mo><</mo></math> 0.0001). No significant difference was observed in hot flashes and joint pain between the dienogest and GnRH agonist groups. However, a significant difference was found in vaginal dryness (p = 0.03) and decreased libido (p = 0.02). GnRH agonists and dienogest reduced irregular vaginal bleeding.</p><p><strong>Conclusion: </strong>Our results suggested that the effect of GnRH agonists and dienogest in improving pelvic pain for endometriosis is the same after a 3-month treatment period. However, these 2 drugs caused different adverse effects.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"22 12","pages":"995-1002"},"PeriodicalIF":1.6,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11830921/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143449019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Outcomes of antibiotic therapy and transvaginal ultrasound-guided efficacy of transvaginal ultrasound-guided drainage in treating tubo-ovarian abscesses: Three case reports.","authors":"Zahra Heidar, Tayebeh Esfidani, Atefeh Moridi, Mahtab Anvari","doi":"10.18502/ijrm.v22i11.17825","DOIUrl":"10.18502/ijrm.v22i11.17825","url":null,"abstract":"<p><strong>Background: </strong>To introduce minimally invasive methods for the successful treatment of tubo-ovarian abscesses (TOAs), an antibiotic regimen was considered the first line of treatment. However, in some cases, this approach fails, and another intervention (laparotomy or minimally guidance drainage) is required.</p><p><strong>Case presentations: </strong>3 women with a history of long-time infertility, all of them were candidates for in vitro fertilization referred to the obstetrics and gynecology department with similar manifestations. For these 3 cases (30-40 yr) the first approach was a broad-spectrum antibiotic therapy. In 2 cases the last step in treatment was transvaginal ultrasound guidance drainage, and in one case laparotomy was done after antibiotic regimen failure; however, in all of 3 cases the best results were seen in transvaginal ultrasound guidance drainage.</p><p><strong>Conclusion: </strong>Patients who have ovarian endometrioma and undergo an assisted reproductive technology cycle, as well as ovum pick up, increase the possibility of TOA occurrence in them. The use of transvaginal ultrasound guidance drainage approach for the treatment of TOA in selective cases, in addition to broad-spectrum antibiotics in patients might reduce their need for invasive treatment with laparotomy.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"22 11","pages":"927-934"},"PeriodicalIF":1.8,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11757675/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143046367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of L-carnitine and pentoxifylline on long-term preservation of the human sperms: An experimental study.","authors":"Elham Aliabadi, Zohre Nateghian, Mohammad Hossein Nasr-Esfahani, Marziyeh Tavalaee, Tahereh Talaei-Khozani","doi":"10.18502/ijrm.v22i11.17820","DOIUrl":"10.18502/ijrm.v22i11.17820","url":null,"abstract":"<p><strong>Background: </strong>In infertility clinics, long-time preserving high-quality spermatozoa is a challenging problem.</p><p><strong>Objective: </strong>The present study aimed to prolong preserving of the human spermatozoa by adding pentoxifylline (PT) and L-carnitine (LC) without using high-cost freezing techniques.</p><p><strong>Materials and methods: </strong>In this experimental study, semen samples of 26 normozoospermia men aged between 28-34 yr, were firstly prepared using the swim-up technique, and each sample was divided into the following 3 aliquots: untreated control group, the LC, and PT-treated groups. The samples were stored for up to 12 days at 4-6 C, and sperm motility was assessed. The percentages of the sperms with double-stranded DNA, apoptotic, and acrosomal interacted sperms were evaluated by sperm chromatin structure assay, AnnexinV-PI staining, and peanut agglutinin, respectively.</p><p><strong>Results: </strong>On day 7, 26.83% <math><mo>±</mo></math> 4.26 of sperms were motile in the PT group which was significantly more than LC (6.67% <math><mo>±</mo></math> 0.61) and control (0.83 <math><mo>±</mo></math> 0.17) groups (p <math><mo><</mo></math> 0.001). At day 12, while all sperms lost their motility in LC and control groups, adding PT led to 3.17% <math><mo>±</mo></math> 0.47 sperms remaining motile (p <math><mo><</mo></math> 0.001). Moreover, on day 12, the percent of apoptotic sperms in the PT-treated group (8% <math><mo>±</mo></math> 0.20) was significantly lower than in LC-treated group (5.9% <math><mo>±</mo></math> 0.28, p = 0.03). None of the additives positively affected the number of sperms with double-stranded DNA (p <math><mo>></mo></math> 0.05). LC could also maintain acrosomal integrity over a storage time of up to 12 days.</p><p><strong>Conclusion: </strong>Despite PT's improved sperm motility, LC was more efficient in preventing apoptosis and acrosomal reactions. However, DNA was resistant to denaturation regardless of the treatments.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"22 11","pages":"871-882"},"PeriodicalIF":1.8,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11757672/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143045888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improving reproductive outcomes in frozen embryo transfer over fresh embryo transfer in women with endometrioma: A historical cohort study.","authors":"Parisa Pirooznia, Mehri Mashayekhi, Firouzeh Ghaffari, Nadia Jahangiri, Zahra Zolfaghari, Firoozeh Ahmadi, Fateme Hasani, Nima Narimani","doi":"10.18502/ijrm.v22i11.17819","DOIUrl":"10.18502/ijrm.v22i11.17819","url":null,"abstract":"<p><strong>Background: </strong>Endometrioma, a common manifestation of endometriosis, often indicates the severity of the disease. In vitro fertilization and embryo transfer (ET) are key therapeutic strategies for infertility associated with endometriosis. However, the optimal type of ET (frozen or fresh) and its impact on pregnancy success rates remain debated, with limited studies available.</p><p><strong>Objective: </strong>This historical cohort study aimed to compare fertility and neonatal outcomes, focusing on live birth rate (LBR), clinical pregnancy, and implantation rates in women with endometrioma-associated infertility, between fresh and frozen embryo transfer (FET).</p><p><strong>Materials and methods: </strong>In this historical cohort study, the medical records (files) of 289 women diagnosed with endometrioma-related infertility, who underwent in vitro fertilization/intracytoplasmic sperm injection treatment at Royan Institute, Tehran, Iran between March 2016-2021 were reviewed. Ultimately, 200 files that met the established criteria were selected for review. The extracted data was then compared between groups: FET (n = 121) and fresh ET (n = 79).</p><p><strong>Results: </strong>No significant differences were observed between the groups in terms of demographic characteristics and endometrioma size. The only significant difference in fertility outcomes was the LBR, which was 36.4% for the FET group compared to 22.8% for the fresh ET group (p = 0.04). No significant differences were observed in neonatal outcomes between the groups. Overall, our study suggests that FET may lead to higher LBRs in women diagnosed with endometrioma.</p><p><strong>Conclusion: </strong>Our study suggests that FET may lead to higher LBRs in women diagnosed with endometrioma.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"22 11","pages":"857-870"},"PeriodicalIF":1.8,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11757671/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143046257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective effects of resveratrol on the expression of <i>catalase, glutathione peroxidase</i>, and <i>superoxide dismutase</i> genes in the ovary and their activity in the serum of rats exposed to lead acetate: An experimental study.","authors":"Mohammad Karimian, Mozhdeh Ghadiri, Seyedeh Mahsa Poormoosavi, Hossein Najafzadehvarzi","doi":"10.18502/ijrm.v22i11.17821","DOIUrl":"10.18502/ijrm.v22i11.17821","url":null,"abstract":"<p><strong>Background: </strong>Lead (Pb) could be toxic to the female reproductive system, and resveratrol (Res) may overcome this toxicity.</p><p><strong>Objective: </strong>To investigate the Res impact on the catalase (<i>Cat</i>), glutathione peroxidase (<i>Gpx</i>), and superoxide dismutase (<i>Sod</i>) gene expression in the ovary and on the Cat and Gpx enzyme activity in the serum of rats exposed to lead acetate.</p><p><strong>Materials and methods: </strong>In this experimental study, 33 female Wistar rats (8-10 wk, 180-200 gr) were divided into 6 groups: a control group (normal saline), a Res group (40 mg/kg), and a Pb group (lead acetate 30 mg/kg). 3 additional groups received lead acetate (30 mg/kg) with Res at 20, 40, and 80 mg/kg for 21 days. Gene expression of <i>Cat</i>, <i>Gpx</i>, and <i>Sod</i> was measured via qPCR, and serum Cat and Gpx activity was assessed using standard methods. Bioinformatics tools were used to evaluate Res effects on gene and protein function.</p><p><strong>Results: </strong>Lead acetate significantly downregulates <i>Cat</i>, <i>Gpx</i>, and <i>Sod</i> gene expression, but Res significantly upregulates gene expression, especially at doses of 40 mg/kg for <i>Cat</i>, 20 mg/kg and 40 mg/kg for <i>Gpx</i>, and 80 mg/kg for <i>Sod</i>. Cat and Gpx enzyme activity increased and decreased in the lead acetate group, respectively. However, Res in all doses decreased only the Cat enzyme activity. Bioinformatics analysis indicates that Res can interact with the promoter regions and cavities of all 3 enzymes.</p><p><strong>Conclusion: </strong>Pb can dysregulate the expression and activity of the studied enzymes. However, the impact of Res is influenced by the dose, with 40 mg/kg frequently being the most effective.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"22 11","pages":"883-894"},"PeriodicalIF":1.8,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11757674/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143046490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}