白藜芦醇对醋酸铅暴露大鼠卵巢过氧化氢酶、谷胱甘肽过氧化物酶和超氧化物歧化酶基因表达及其血清活性的保护作用:实验研究。

IF 1.6 Q3 OBSTETRICS & GYNECOLOGY
International Journal of Reproductive Biomedicine Pub Date : 2024-01-10 eCollection Date: 2024-11-01 DOI:10.18502/ijrm.v22i11.17821
Mohammad Karimian, Mozhdeh Ghadiri, Seyedeh Mahsa Poormoosavi, Hossein Najafzadehvarzi
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引用次数: 0

摘要

背景:铅(Pb)可能对女性生殖系统有毒性,而白藜芦醇(Res)可以克服这种毒性。目的:探讨Res对醋酸铅暴露大鼠卵巢过氧化氢酶(Cat)、谷胱甘肽过氧化物酶(Gpx)、超氧化物歧化酶(Sod)基因表达及血清Cat、Gpx酶活性的影响。材料与方法:选用雌性Wistar大鼠33只(8 ~ 10周,180 ~ 200 gr),分为6组:对照组(生理盐水)、Res组(40 mg/kg)、Pb组(醋酸铅30 mg/kg)。另外3组服用醋酸铅(30 mg/kg),剂量分别为20、40和80 mg/kg,连续21 d。采用qPCR检测Cat、Gpx和Sod基因表达,采用标准方法检测血清Cat和Gpx活性。使用生物信息学工具评估Res对基因和蛋白质功能的影响。结果:乙酸铅显著下调Cat、Gpx和Sod基因表达,而Res显著上调Cat、Gpx和Gpx分别为40 mg/kg、20 mg/kg和40 mg/kg, Sod为80 mg/kg。Cat和Gpx酶活性在乙酸铅组分别升高和降低。然而,所有剂量的Res仅降低Cat酶活性。生物信息学分析表明,Res可与3种酶的启动子区和空腔相互作用。结论:铅可调节上述酶的表达和活性。然而,Res的影响受剂量的影响,40 mg/kg通常是最有效的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Protective effects of resveratrol on the expression of catalase, glutathione peroxidase, and superoxide dismutase genes in the ovary and their activity in the serum of rats exposed to lead acetate: An experimental study.

Background: Lead (Pb) could be toxic to the female reproductive system, and resveratrol (Res) may overcome this toxicity.

Objective: To investigate the Res impact on the catalase (Cat), glutathione peroxidase (Gpx), and superoxide dismutase (Sod) gene expression in the ovary and on the Cat and Gpx enzyme activity in the serum of rats exposed to lead acetate.

Materials and methods: In this experimental study, 33 female Wistar rats (8-10 wk, 180-200 gr) were divided into 6 groups: a control group (normal saline), a Res group (40 mg/kg), and a Pb group (lead acetate 30 mg/kg). 3 additional groups received lead acetate (30 mg/kg) with Res at 20, 40, and 80 mg/kg for 21 days. Gene expression of Cat, Gpx, and Sod was measured via qPCR, and serum Cat and Gpx activity was assessed using standard methods. Bioinformatics tools were used to evaluate Res effects on gene and protein function.

Results: Lead acetate significantly downregulates Cat, Gpx, and Sod gene expression, but Res significantly upregulates gene expression, especially at doses of 40 mg/kg for Cat, 20 mg/kg and 40 mg/kg for Gpx, and 80 mg/kg for Sod. Cat and Gpx enzyme activity increased and decreased in the lead acetate group, respectively. However, Res in all doses decreased only the Cat enzyme activity. Bioinformatics analysis indicates that Res can interact with the promoter regions and cavities of all 3 enzymes.

Conclusion: Pb can dysregulate the expression and activity of the studied enzymes. However, the impact of Res is influenced by the dose, with 40 mg/kg frequently being the most effective.

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来源期刊
CiteScore
2.40
自引率
7.70%
发文量
93
审稿时长
16 weeks
期刊介绍: The International Journal of Reproductive BioMedicine (IJRM), formerly published as "Iranian Journal of Reproductive Medicine (ISSN: 1680-6433)", is an international monthly scientific journal for who treat and investigate problems of infertility and human reproductive disorders. This journal accepts Original Papers, Review Articles, Short Communications, Case Reports, Photo Clinics, and Letters to the Editor in the fields of fertility and infertility, ethical and social issues of assisted reproductive technologies, cellular and molecular biology of reproduction including the development of gametes and early embryos, assisted reproductive technologies in model system and in a clinical environment, reproductive endocrinology, andrology, epidemiology, pathology, genetics, oncology, surgery, psychology, and physiology. Emerging topics including cloning and stem cells are encouraged.
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