International Journal of Reproductive Biomedicine最新文献

{"title":"Platelet to lymphocyte and neutrophil to lymphocyte ratio in the first trimester of pregnancy, are they useful for predicting spontaneous miscarriage? A case-control study.","authors":"Maryam Yazdizadeh,&nbsp;Nafisseh Hivehchi,&nbsp;Marjan Ghaemi,&nbsp;Sepideh Azizi,&nbsp;Maryam Saeedzarandi,&nbsp;Narjes Afrooz,&nbsp;Pegah Keshavarz,&nbsp;Melika Ansarin,&nbsp;Maliheh Fakehi,&nbsp;Mina Yazdizadeh,&nbsp;Mojgan Mokhtari","doi":"10.18502/ijrm.v21i6.13632","DOIUrl":"https://doi.org/10.18502/ijrm.v21i6.13632","url":null,"abstract":"<p><strong>Background: </strong>In 15% of all clinical pregnancies, a miscarriage can occur, but the exact cause of this phenomenon is not fully understood. However, it is believed that a faulty placenta, which triggers an inflammatory response in the mother's body, may be one of the causes. Medical literature has increasingly focused on 2 indicators of inflammation, the platelet-lymphocyte ratio (PLR) and neutrophil-lymphocyte ratio (NLR). Despite this, there has yet to be a study conducted that examines the rates of PLR and NLR in cases of miscarriage.</p><p><strong>Objective: </strong>This study aims to determine whether there is an increase in complete blood count inflammatory parameters such as NLR and PLR in women who experience miscarriages.</p><p><strong>Materials and methods: </strong>This retrospective case-control study was conducted from March 2021 to March 2022, across 3 academic hospitals in Tehran, Iran. A total of 240 participants were enrolled comprising individuals with either miscarriages or normal pregnancies (n = 120/each). Data were collected from the medical records of participants aged between 18-42 yr old, with gestational age ranging from 6-13 wk. The demographic information, including age, body mass index, parity, history of abortion, number of abortions, number of living children, hematocrit and hemoglobin levels, platelet distribution width (PDW), PLR, NLR, mean platelet volume, and platelet were extracted from their records. The gestational age was also recorded.</p><p><strong>Results: </strong>A total of 240 participants were recruited for the study. PDW, NLR, PLR, and lymphocyte values were higher in the miscarriage group compared to the healthy normal pregnant women (p <math><mo><</mo></math> 0.001). Mean platelet volumes were found to be lower in the miscarriage group compared to the healthy normal pregnant women (p <math><mo><</mo></math> 0.001).</p><p><strong>Conclusion: </strong>Although, no statistically significant difference was observed in the hemoglobin, hematocrit, platelets, and neutrophils in these 2 groups of pregnant women. The higher inflammatory markers including PDW, NLR, and PLR could potentially aid in the speculation of defective placentation as a contributing factor to the development of miscarriage. Measurement of these markers may be useful to predict pregnancy leading to miscarriage.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"21 6","pages":"463-470"},"PeriodicalIF":1.3,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10407914/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9976209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Testosterone propionate and <i>Swarna Bhasma</i> treatment modulated D-galactose induced reproductive alterations in male Wistar rats: An experimental study.","authors":"Aashish Kumar Netam, Vikas Pankaj Bhargava, Rambir Singh, Poonam Sharma","doi":"10.18502/ijrm.v21i4.13270","DOIUrl":"10.18502/ijrm.v21i4.13270","url":null,"abstract":"<p><strong>Background: </strong>The male reproductive system undergoes several adverse age-related changes like decreased hormone synthesis, sperm count, and testicular alteration that can impact on fertility.</p><p><strong>Objective: </strong>The study aims to investigate the effects of testosterone propionate (TP), and ayurvedic formulation <i>Swarna Bhasma</i> (SB) on D-galactose (D-gal) induced reproductive aging in male Wistar rats.</p><p><strong>Materials and methods: </strong>60 male Wistar rats were divided into 10 groups of 6 animals. Reproductive aging was induced by D-gal (150 mg/kg Bwt) exposure for 60 days. The rats were then treated by post and combination treatment with TP (2 mg/kg Bwt) and SB (6.75 mg/kg Bwt). Then sperm parameters, reproductive hormones, inflammatory markers, testicular antioxidant enzymes, steroidogenic enzymes, and histological manifestation of testis were evaluated.</p><p><strong>Results: </strong>Exposure of D-gal caused significant (p <math><mo><</mo></math> 0.001) decrease in serum testosterone (T), testicular steroidogenic, and antioxidant enzymes. Administration of TP increased the serum T level, testicular antioxidant enzymes, and spermatogenic profile at a significant level of (p <math><mo><</mo></math> 0.001) compared to D-gal. Further, the SB treatment significantly (p <math><mo><</mo></math> 0.001) elevated the serum T level, sperm count, testicular antioxidant enzymes, steroidogenic enzymes, when compared to D-gal.</p><p><strong>Conclusion: </strong>Both the treatment of TP and SB treatments recovered the reproductive impairments caused by D-gal. However, exogenous T supplementation via TP administration is associated with various side effects during long-term use. SB is an Ayurvedic formulation having a long history of usage in India. The current findings suggest that the SB may be used as a good alternative for potentiating reproductive function in aging males.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"21 4","pages":"303-322"},"PeriodicalIF":1.6,"publicationDate":"2023-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10227353/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9566936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of leaf hydroalcoholic extract of Ephedra pachyclada infertility in male rats treated with cyclophosphamide: An experimental study.","authors":"Shiva Saleh,&nbsp;Aref Ghanaatpisheh,&nbsp;Hoda Haghshenas,&nbsp;Negar Parvin,&nbsp;Elmira Mikaeiliagah,&nbsp;Hossein Kargar Jahromi,&nbsp;Bahare Ebrahimi","doi":"10.18502/ijrm.v21i4.13268","DOIUrl":"https://doi.org/10.18502/ijrm.v21i4.13268","url":null,"abstract":"<p><strong>Background: </strong>Cyclophosphamide (CP) has clinical applications in treating diverse malignancies and autoimmune disorders; at the same time, it also has harmful effects on the body tissues, particularly the genitals. The most significant side effects of CP are changing the reproductive system's function and infertility.</p><p><strong>Objective: </strong>This study determines the Ephedra hydroalcoholic extract (EP) role on testicular tissue and the pituitary-gonadal axis in CP-treated male rats.</p><p><strong>Materials and methods: </strong>In this experimental study, 48 adult Wistar rats were separated into 6 groups (n = 8/each): control, sham, CP recipients, and CP recipients with gavage-fed EP (250, 500, and 1000 mg/kg). On the 29<math><msup><mrow></mrow><mtext>th</mtext></msup></math> day, the blood of the weighed animals' was drawn from their heart, and serum concentrations of follicle-stimulating hormone, luteinizing hormone, and testosterone were measured. After preparing testicular tissue segments, cells were counted.</p><p><strong>Results: </strong>While CP decreased follicle-stimulating hormone, luteinizing hormone, and testosterone levels (p <math><mo><</mo></math> 0.05), the use of EP changed them and even reached the control. Serum gonadotropin-releasing hormone increased significantly in all EP groups compared to the control and CP groups. Compared to the control, a significant decrease in total antioxidant capacity and plasma glutathione peroxidase was observed in the CP groups. EP (all doses) significantly increased their concentration compared to the CP group (p <math><mo><</mo></math> 0.05); significant reduction in serum total oxidant status and malondialdehyde in CP groups changed by EP (p <math><mo><</mo></math> 0.05). Although CP's role on spermatogonia counts (57.5 <math><mo>±</mo></math> 5.2 in CP, 67.1 <math><mo>±</mo></math> 6.0 in control), higher doses of EP had no significant effect on this but did affect spermatocyte and spermatid cells count.</p><p><strong>Conclusion: </strong>Due to its antioxidant characteristics, EP mitigated the effects of CP on the investigated parameters in rats.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"21 4","pages":"285-294"},"PeriodicalIF":1.3,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10227357/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9570840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of nifedipine with and without sildenafil citrate for the management of preterm labor in pregnant women: A randomized clinical trial.","authors":"Shahla Nasrolahei,&nbsp;Seyedeh Arezoo Hoseini,&nbsp;Seyedeh Azadeh Hosseini,&nbsp;Seyedeh Narjes Khatoon Hosseini,&nbsp;Seyedeh Sahar Hosseini,&nbsp;Parsa Moradian Lotfi","doi":"10.18502/ijrm.v21i5.13471","DOIUrl":"https://doi.org/10.18502/ijrm.v21i5.13471","url":null,"abstract":"<p><strong>Background: </strong>Preterm labor is one of the main causes of neonatal mortality and its treatment is still challenging.</p><p><strong>Objective: </strong>The study aimed to compare the effectiveness of nifedipine (Nif) with and without sildenafil citrate (SC) for the treatment of preterm labor in pregnant women.</p><p><strong>Materials and methods: </strong>In this clinical trial study, 126 pregnant women referred to the Fatemieh hospital, Hamadan, Iran with a complaint of preterm labor were evaluated. Participants were randomly divided into 2 groups of Nif 20 mg orally (single dose), then 10 mg every 6-hr, and at the same time vaginal SC 25 mg every 8 hr (Nif + SC) or Nif alone. Treatment was continued for 48-72 hr if uterine contractions did not resolve in both groups. Delivery rates at the time of hospitalization and neonatal outcome were compared between the 2 groups.</p><p><strong>Results: </strong>No statistically significant difference was observed between the 2 study groups in terms of mean age, gestational age, body mass index, and parity. 76.2% of Nif + SC participants in the first 72 hr of hospitalization and 57.2% of Nif participants remained without delivery (p = 0.02). The neonatal hospitalization rate of the Nif + SC group in the neonatal intensive care unit was 25.4% and in the Nif group was 42.9% (p = 0.03).</p><p><strong>Conclusion: </strong>Nif with SC is superior to Nif alone in women at risk of preterm labor due to increasing gestational age and better neonatal outcomes.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"21 5","pages":"379-386"},"PeriodicalIF":1.3,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10285191/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10075317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Does dual trigger improve euploidy rate in normoresponder? A cross-sectional study.","authors":"Sule Yildirim Kopuk,&nbsp;Zeynep Ece Utkan Korun,&nbsp;Aysen Yuceturk,&nbsp;Ozge Karaosmanoglu,&nbsp;Caglar Yazicioglu,&nbsp;Bulent Tiras,&nbsp;Yigit Cakiroglu","doi":"10.18502/ijrm.v21i5.13473","DOIUrl":"https://doi.org/10.18502/ijrm.v21i5.13473","url":null,"abstract":"<p><strong>Background: </strong>With the introduction of the dual triggering-gonadotropin-releasing hormone (GnRH) analog and recombinant human chorionic gonadotropin (hCG) combination, women with a history of low mature oocyte proportion and empty follicle syndrome were shown to benefit from the dual trigger.</p><p><strong>Objective: </strong>To investigate whether dual triggering of oocyte maturation with a GnRH agonist (GnRHa) combined with hCG can affect the euploidy rate and improve in vitro fertilization outcomes for normoresponder women.</p><p><strong>Materials and methods: </strong>In this cross-sectional study, 494 women who underwent controlled ovarian stimulation with hCG (n = 274) or dual triggering (hCG+GnRHa, n = 220) at Acibadem Maslak hospital, Assisted Reproductive Unit, from January 2019-2022 were enrolled in this study. Preimplantation genetic testing for aneuploidy was performed on all participants.</p><p><strong>Results: </strong>Both groups had similar baseline and clinical characteristics. Of the 881 embryos biopsied, 312 (35.4%) were reported as euploid in the hCG trigger group; in the dual trigger group, 186 (29.8%) of 623 screening embryos were reported as euploid. The hCG group had a higher euploidy rate per biopsied embryo, although the difference was not statistically significant (31.4 <math><mo>±</mo></math> 26.5 vs. 26.5 <math><mo>±</mo></math> 33.3, p <math><mo>></mo></math> 0.05).</p><p><strong>Conclusion: </strong>In normoresponders, adding GnRHa for final follicular maturation to hCG did not improve the euploidy rate.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"21 5","pages":"395-402"},"PeriodicalIF":1.3,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10285196/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10075320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fresh embryo transfer in the cleavage and blastocyst stages and pregnancy outcomes: A retrospective cross-sectional study.","authors":"Seyedeh Mahsa Poormoosavi,&nbsp;Mohammad Amin Behmanesh,&nbsp;Sara Aryannejad,&nbsp;Sima Janati","doi":"10.18502/ijrm.v21i5.13477","DOIUrl":"https://doi.org/10.18502/ijrm.v21i5.13477","url":null,"abstract":"<p><strong>Background: </strong>Advances in cell culture media have led to a trend of embryo transfer from the early cleavage stages to blastocyst stage.</p><p><strong>Objective: </strong>The study aims to compare the effects of fresh embryo transfer in cleavage and blastocyst stage on pregnancy outcomes.</p><p><strong>Materials and methods: </strong>This cross-sectional study was conducted on 1422 cases referred to the Umm-al-Banin Infertility Clinic Center, Dezful, Iran, between July 2013 and December 2020 who were candidates for in vitro fertilization/intracytoplasmic sperm injection for fresh embryo transfer. A total of 1246 cases were divided into 4 categories on days 2-5 or 6. Chemical and clinical pregnancy, abortion, multifetal pregnancy, ongoing pregnancy, and live birth rates were analyzed.</p><p><strong>Results: </strong>Fresh embryo transfer was performed in 28.5% of the cases on the 2<math><msup><mrow></mrow><mtext>nd</mtext></msup></math> day, 45.8% on the 3<math><msup><mrow></mrow><mtext>rd</mtext></msup></math>, 15.3% on the 4<math><msup><mrow></mrow><mtext>th</mtext></msup></math> day, and 10.4% on days 5 or 6. The overall clinical pregnancy and live birth rates were estimated at 20.6% and 17.6% in the cleavage, and 17% and 14% in the blastocyst stages, respectively. However, no significant difference was observed in either group. In addition, there was no significant difference between groups in terms of the abortion, multifetal pregnancy, and ongoing pregnancy rate (p <math><mo>></mo></math> 0.05).</p><p><strong>Conclusion: </strong>According to the results, the consequences of pregnancy in fresh embryo transfer at the blastocyst stage were not superior to embryo transfer at different stages of the cleavage process.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"21 5","pages":"425-432"},"PeriodicalIF":1.3,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10285195/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9719864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intrauterine administration of autologous peripheral blood mononuclear cells regulates the endometrium estrogen and progesterone receptor expression: An RCT.","authors":"Fattaneh Farifteh,&nbsp;Elham Fazeli,&nbsp;Seyedeh Zeinab Hosseini,&nbsp;Seyedeh Soheila Arefi,&nbsp;Ashraf Moini,&nbsp;Robabeh Taheripanah,&nbsp;Fatemeh Rouhollah,&nbsp;Mohammad Salehi,&nbsp;Ahmad Hosseini,&nbsp;Moncef Benkhalifa","doi":"10.18502/ijrm.v21i4.13273","DOIUrl":"https://doi.org/10.18502/ijrm.v21i4.13273","url":null,"abstract":"<p><strong>Background: </strong>Repeated implantation failure (RIF) affects 15% of women of reproductive age. There is a high endometrial expression of both estrogen receptors and progesterone receptors (PRs) during the window of implantation in women with RIF.</p><p><strong>Objective: </strong>To evaluate the effects of intrauterine administration of human peripheral blood mononuclear cells (PBMC) on estrogen receptor α (ERα) and PRs expression in the endometrium of women with RIF during the implantation window.</p><p><strong>Materials and methods: </strong>This randomized clinical trial study was conducted on 22 women with RIF history from January 2018 to August 2019 in Erfan hospital, Tehran, Iran. Participantswere divided into 2 groups (PBMC-treated group [n = 11] and control group [n = 11]). Endometrial tissue samples were collected at the implantation window time, during the mid-secretory phase (luteinizing hormone surge +7 days) of each menstrual cycle. The quantitative real-time polymerase chain reaction technique was used to measure the mRNA levels of ERα and PRs isoforms (PR-A and PR-B) in endometrial tissues. Furthermore, the protein expression of ERα and PRs was investigated using immunohistochemical staining.</p><p><strong>Results: </strong>PBMC treatment significantly decreased the mRNA expression of endometrial ERα and PRs isoforms at the time of the implantation window (p <math><mo><</mo></math> 0.001). Moreover, the endometrial ERα and PRs protein localization were significantly lower in PBMC-treated women compared with controls (p = 0.01, and p <math><mo><</mo></math> 0.001 respectively).</p><p><strong>Conclusion: </strong>The intrauterine administration of PBMC decreased the endometrial ERα and PRs expression during the window of implantation in women with RIF. This local response to PBMC therapy could promote endometrial receptivity and embryo implantation.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"21 4","pages":"343-354"},"PeriodicalIF":1.3,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10227356/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9558572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A new hypothesis may explain human parthenogenesis and ovarian teratoma: A review study.","authors":"Abdelmonem Awad Hegazy,&nbsp;Aiman Ibraheem Al-Qtaitat,&nbsp;Raafat Awad Hegazy","doi":"10.18502/ijrm.v21i4.13267","DOIUrl":"https://doi.org/10.18502/ijrm.v21i4.13267","url":null,"abstract":"<p><p>Parthenogenesis (PG) is a rare phenomenon occurring in humans, and understanding this may help us develop an explanation for such occurrences. Moreover, it may help reveal the cause of idiopathic ovarian teratoma (OT). We aim to explain the occurrence of PG and OT in humans based on a new hypothesis. Previous literature has been searched through relevant scientific websites and international journals on the causes and mechanisms of PG and OT in humans. The previous literature on human PG was sparse and mostly contained case reports. It appears that human PG is not as rare as previously reported but may occur spontaneously, resulting in OT formation. The difference between PG and sexual reproduction is that PG has no embryonic diversity. The biopsied embryonic samples in the PG correspond exclusively to those of the maternal side. Spontaneous PG in humans often degrades or leads to formation of OT. The cause and mechanism of spontaneous PG remain unclear in the available literature. Here, we hypothesized that in some cases the secondary oocyte and first polar body enclosed in the zona pellucida may fuse together to form a single cell that restores the diploid number of chromosomes and initiates cell division to form PG. It may go unnoticed or be represented by the OT. Future studies are recommended to investigate this hypothesis.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"21 4","pages":"277-284"},"PeriodicalIF":1.3,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10227352/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9566937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of mouse ovarian follicular development and gene expression in the presence of ovarian tissue extract and sodium selenite: An experimental study.","authors":"Hamed Shoorei,&nbsp;Mina Jafarabadi,&nbsp;Shahram PourBayranvand,&nbsp;Mojdeh Salehnia","doi":"10.18502/ijrm.v21i5.13476","DOIUrl":"https://doi.org/10.18502/ijrm.v21i5.13476","url":null,"abstract":"<p><strong>Background: </strong>Ovarian tissue extract (OTE) and sodium selenite (SS) enhance the growth and maturation of preantral follicles in a dose-dependent manner.</p><p><strong>Objective: </strong>The present study was designed to bring more information regarding the mechanism of OTE and SS on the mRNA expression of follicle-stimulating hormone receptors (FSHR) and the proliferation cell nuclear antigens (PCNA) of in vitro matured isolated follicles.</p><p><strong>Materials and methods: </strong>The tissue extract was prepared from adult ovaries. The preantral follicles (n = 266) were isolated from 12-16-day-old mice and cultured in the control, experimental I (10 ng/ml SS), and experimental II (OTE) groups for 12 days. The follicular diameter, survival, and maturation rates, also, the production of 17-β-estradiol and progesterone, and the follicular expression of <i>PCNA</i> and <i>FSH</i> receptor genes were analyzed.</p><p><strong>Results: </strong>The survival rate of follicles in the SS-treated group (84.58%) was significantly higher than that OTE (75.63%; p = 0.023) and control (69.38%; p = 0.032) groups. The mean diameter of culture follicles in experimental group I (403.8 μm) and experimental group II (383.97 μm) increased significantly in comparison with the control group (342.05 μm; p = 0.032). The developmental rate of follicles, percentages of antrum formation, released metaphase II oocytes (p = 0.027; p = 0.019 respectively), production of hormones and the expression of 2 studied genes were significantly increased in both experimental groups in compare with control group (p = 0.021; p = 0.023 respectively).</p><p><strong>Conclusion: </strong>The OTE and SS have a positive effect on development of mouse preantral follicles via over-expression of <i>FSHR</i> and <i>PCNA</i> genes.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"21 5","pages":"415-424"},"PeriodicalIF":1.3,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10285190/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9707644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Circulating serum preptin levels in women with polycystic ovary syndrome: A systematic review and meta-analysis.","authors":"Seyed Sobhan Bahreiny,&nbsp;Elnaz Harooni,&nbsp;Mohammad Reza Dabbagh,&nbsp;Reza Ebrahimi","doi":"10.18502/ijrm.v21i5.13470","DOIUrl":"https://doi.org/10.18502/ijrm.v21i5.13470","url":null,"abstract":"<p><strong>Background: </strong>Polycystic ovary syndrome (PCOS) is a prevalent endocrine disorder with complex pathogenesis and metabolic complications, such as insulin resistance. Among the new markers, preptin seems to play a significant role in metabolic disorders.</p><p><strong>Objective: </strong>This meta-analysis was conducted to determine the relationship between circulating preptin levels and PCOS.</p><p><strong>Materials and methods: </strong>A systematic review and meta-analysis was performed to identify relevant articles in electronic databases such as PubMed, Web of Science, Scopus, Cochrane, EMBASE, and the Google Scholar search engine, using a predefined search strategy. A random-effects model was used to combine standard mean difference (SMD) and 95% CI to compare results between groups. Meta-regression and subgroup analysis were also performed to reveal the sources of heterogeneity.</p><p><strong>Results: </strong>The meta-analysis encompassed a total of 8 studies and 582 participants. The results indicate a statistically significant association between PCOS and serum preptin levels, with a pooled standardized mean difference (SMD = 1.35; 95% CI]: 0.63-2.08; p <math><mo><</mo></math> 0.001). Further analysis suggested a significant difference in serum preptin levels between women with PCOS and higher homeostatic model assessment for insulin resistance ratio (SMD = 2.40; 95% CI: 1.17-3.63; p <math><mo><</mo></math> 0.001) within the subgroup.</p><p><strong>Conclusion: </strong>Our meta-analysis shows that increased serum preptin levels are associated with PCOS, suggesting that preptin may be related to the pathogenesis of PCOS and may be recognized as a novel diagnostic biomarker for PCOS. However, further studies are needed to confirm our results.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"21 5","pages":"367-378"},"PeriodicalIF":1.3,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10285194/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10075315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}