International Journal of Reproductive Biomedicine最新文献

{"title":"Increased expression level of <i>Dicer</i> in placenta is associated with the early onset of preeclampsia.","authors":"Fatemeh Mohseni, Mohammad Shekari, Kianoosh Malekzadeh, Pooneh Nikuei, Fatemeh Poordarvish, Ali Atashabparvar, Kourosh Bamdad","doi":"10.18502/ijrm.v21i12.15043","DOIUrl":"10.18502/ijrm.v21i12.15043","url":null,"abstract":"","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"21 12","pages":"1031-1034"},"PeriodicalIF":1.3,"publicationDate":"2024-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10869960/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139899868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Parental consanguinity and ovarian reserve: A retrospective cohort study.","authors":"Shadya Al Saeghi, Tamadhir Al-Mahrouqi, Maha Al-Khadhuri, Rahma Al-Ghabshi, Jokha Al-Sabti, Sachin Jose, Vaidyanathan Gowri","doi":"10.18502/ijrm.v21i12.15039","DOIUrl":"10.18502/ijrm.v21i12.15039","url":null,"abstract":"<p><strong>Background: </strong>Infertility affects around 10-15% of couples worldwide and is both a social and medical problem. Parental consanguinity is considered to reduce fertility reserve. Consanguineous marriages, especially first cousin marriages, are very common in Oman according to the Oman National Health Survey data.</p><p><strong>Objective: </strong>This study aimed to determine whether women born to consanguineous parents have reduced ovarian reserve.</p><p><strong>Materials and methods: </strong>This cohort study was conducted on 414 women aged <math><mo>≤</mo></math> 39, treated for infertility at Sultan Qaboos University hospital and Royal hospital, Muscat, Oman from January 2019-December 2020. Each participant was interviewed and a complete history, including parental consanguinity and physical examination, were recorded. On day 2 of the menstrual cycle, serum concentration of the following was performed: follicle-stimulating hormone (FSH), luteinizing hormone, estradiol, prolactin, thyroid stimulating hormone, and anti-Müllerian hormone (AMH). AMH was done, if necessary, on other days of the cycle. Antral follicle count (AFC) was done on day 2 and 3 of the menstrual cycle.</p><p><strong>Results: </strong>Of the 414 women, parental consanguinity was present in 40.2% of couples. In women with low AFC, parental consanguinity was present in 15.3% compared to 13.0% in the non-consanguineous group. About 15% of women with low AMH had consanguineous parents, compared to 20.2% from the non-consanguineous group. High levels of FSH were present in 6.5% and 4.2% of the consanguineous and non-consanguineous groups, respectively. No significant difference was observed in AFC with reference to body mass index.</p><p><strong>Conclusion: </strong>The results from this study showed no statistically significant difference in low ovarian reserves (AFC, AMH, and FSH) in women whose parents had a consanguineous marriage.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"21 12","pages":"1013-1020"},"PeriodicalIF":1.3,"publicationDate":"2024-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10869961/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139899869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The male-focused marital relationship enrichment and sexual well-being interventions: A scoping review.","authors":"Solmaz Abdollahzadeh Sardehaei, Effat Merghati Khoei, Beheshteh Niusha, Zahed Rezaei","doi":"10.18502/ijrm.v21i12.15035","DOIUrl":"10.18502/ijrm.v21i12.15035","url":null,"abstract":"<p><strong>Background: </strong>There is an increasing need for sexual well-being and health promotion strategies that effectively engage men. Researchers have evaluated the efficacy of sexual and reproductive health-related and marital enhancement interventions in male-dominated societies. However, few have focused on culturally appropriate and gender-specific program effects.</p><p><strong>Objective: </strong>This review aims to evaluate evidence of existing interventions aimed at enriching marital relationships and sexual well-being in adult men.</p><p><strong>Materials and methods: </strong>This scoping review was conducted by searching various databases (CINAHL, PsycINFO, EMBASE, Google Scholar, PubMed, Scopus, SID, and Noormags), and other available resources in both English and Persian languages. We located all publications up to January 2023 with no time restriction. Inclusion criteria were studies targeting men in the enrichment of marital and sexual relationships, which focus on marital/sexual enrichment, sexual and reproductive health (SRH) program, passionate relationship, or sexual satisfaction as the main outcomes. PRISMA guidelines were utilized in this review.</p><p><strong>Results: </strong>Of records 34,405 retrieved by searching, after removing duplicate articles, 8 articles were included based on the inclusion criteria. SRH program was the main focus of 6 articles. Marital and sexual enhancement were common areas of focus in 2 studies. Research methods included 2 semi-experimental, 3 clinical trials, 1 systematic review, 1 content analysis, and a mixed method. According to the assessment result, 4 articles were moderate and 4 were of high quality.</p><p><strong>Conclusion: </strong>Our findings reveal that a small number of interventions specifically offering SRH or marital and sexual enhancement programs to men should be conducted. As the majority were heterosexual couples, we recommend male-focused programs recognizing men's sexuality.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"21 12","pages":"975-984"},"PeriodicalIF":1.6,"publicationDate":"2024-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10869958/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139899872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Outcomes of antibiotic therapy and transvaginal ultrasound-guided efficacy of transvaginal ultrasound-guided drainage in treating tubo-ovarian abscesses: Three case reports.","authors":"Zahra Heidar, Tayebeh Esfidani, Atefeh Moridi, Mahtab Anvari","doi":"10.18502/ijrm.v22i11.17825","DOIUrl":"10.18502/ijrm.v22i11.17825","url":null,"abstract":"<p><strong>Background: </strong>To introduce minimally invasive methods for the successful treatment of tubo-ovarian abscesses (TOAs), an antibiotic regimen was considered the first line of treatment. However, in some cases, this approach fails, and another intervention (laparotomy or minimally guidance drainage) is required.</p><p><strong>Case presentations: </strong>3 women with a history of long-time infertility, all of them were candidates for in vitro fertilization referred to the obstetrics and gynecology department with similar manifestations. For these 3 cases (30-40 yr) the first approach was a broad-spectrum antibiotic therapy. In 2 cases the last step in treatment was transvaginal ultrasound guidance drainage, and in one case laparotomy was done after antibiotic regimen failure; however, in all of 3 cases the best results were seen in transvaginal ultrasound guidance drainage.</p><p><strong>Conclusion: </strong>Patients who have ovarian endometrioma and undergo an assisted reproductive technology cycle, as well as ovum pick up, increase the possibility of TOA occurrence in them. The use of transvaginal ultrasound guidance drainage approach for the treatment of TOA in selective cases, in addition to broad-spectrum antibiotics in patients might reduce their need for invasive treatment with laparotomy.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"22 11","pages":"927-934"},"PeriodicalIF":1.6,"publicationDate":"2024-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11757675/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143046367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of L-carnitine and pentoxifylline on long-term preservation of the human sperms: An experimental study.","authors":"Elham Aliabadi, Zohre Nateghian, Mohammad Hossein Nasr-Esfahani, Marziyeh Tavalaee, Tahereh Talaei-Khozani","doi":"10.18502/ijrm.v22i11.17820","DOIUrl":"10.18502/ijrm.v22i11.17820","url":null,"abstract":"<p><strong>Background: </strong>In infertility clinics, long-time preserving high-quality spermatozoa is a challenging problem.</p><p><strong>Objective: </strong>The present study aimed to prolong preserving of the human spermatozoa by adding pentoxifylline (PT) and L-carnitine (LC) without using high-cost freezing techniques.</p><p><strong>Materials and methods: </strong>In this experimental study, semen samples of 26 normozoospermia men aged between 28-34 yr, were firstly prepared using the swim-up technique, and each sample was divided into the following 3 aliquots: untreated control group, the LC, and PT-treated groups. The samples were stored for up to 12 days at 4-6 C, and sperm motility was assessed. The percentages of the sperms with double-stranded DNA, apoptotic, and acrosomal interacted sperms were evaluated by sperm chromatin structure assay, AnnexinV-PI staining, and peanut agglutinin, respectively.</p><p><strong>Results: </strong>On day 7, 26.83% <math><mo>±</mo></math> 4.26 of sperms were motile in the PT group which was significantly more than LC (6.67% <math><mo>±</mo></math> 0.61) and control (0.83 <math><mo>±</mo></math> 0.17) groups (p <math><mo><</mo></math> 0.001). At day 12, while all sperms lost their motility in LC and control groups, adding PT led to 3.17% <math><mo>±</mo></math> 0.47 sperms remaining motile (p <math><mo><</mo></math> 0.001). Moreover, on day 12, the percent of apoptotic sperms in the PT-treated group (8% <math><mo>±</mo></math> 0.20) was significantly lower than in LC-treated group (5.9% <math><mo>±</mo></math> 0.28, p = 0.03). None of the additives positively affected the number of sperms with double-stranded DNA (p <math><mo>></mo></math> 0.05). LC could also maintain acrosomal integrity over a storage time of up to 12 days.</p><p><strong>Conclusion: </strong>Despite PT's improved sperm motility, LC was more efficient in preventing apoptosis and acrosomal reactions. However, DNA was resistant to denaturation regardless of the treatments.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"22 11","pages":"871-882"},"PeriodicalIF":1.6,"publicationDate":"2024-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11757672/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143045888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improving reproductive outcomes in frozen embryo transfer over fresh embryo transfer in women with endometrioma: A historical cohort study.","authors":"Parisa Pirooznia, Mehri Mashayekhi, Firouzeh Ghaffari, Nadia Jahangiri, Zahra Zolfaghari, Firoozeh Ahmadi, Fateme Hasani, Nima Narimani","doi":"10.18502/ijrm.v22i11.17819","DOIUrl":"10.18502/ijrm.v22i11.17819","url":null,"abstract":"<p><strong>Background: </strong>Endometrioma, a common manifestation of endometriosis, often indicates the severity of the disease. In vitro fertilization and embryo transfer (ET) are key therapeutic strategies for infertility associated with endometriosis. However, the optimal type of ET (frozen or fresh) and its impact on pregnancy success rates remain debated, with limited studies available.</p><p><strong>Objective: </strong>This historical cohort study aimed to compare fertility and neonatal outcomes, focusing on live birth rate (LBR), clinical pregnancy, and implantation rates in women with endometrioma-associated infertility, between fresh and frozen embryo transfer (FET).</p><p><strong>Materials and methods: </strong>In this historical cohort study, the medical records (files) of 289 women diagnosed with endometrioma-related infertility, who underwent in vitro fertilization/intracytoplasmic sperm injection treatment at Royan Institute, Tehran, Iran between March 2016-2021 were reviewed. Ultimately, 200 files that met the established criteria were selected for review. The extracted data was then compared between groups: FET (n = 121) and fresh ET (n = 79).</p><p><strong>Results: </strong>No significant differences were observed between the groups in terms of demographic characteristics and endometrioma size. The only significant difference in fertility outcomes was the LBR, which was 36.4% for the FET group compared to 22.8% for the fresh ET group (p = 0.04). No significant differences were observed in neonatal outcomes between the groups. Overall, our study suggests that FET may lead to higher LBRs in women diagnosed with endometrioma.</p><p><strong>Conclusion: </strong>Our study suggests that FET may lead to higher LBRs in women diagnosed with endometrioma.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"22 11","pages":"857-870"},"PeriodicalIF":1.6,"publicationDate":"2024-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11757671/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143046257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective effects of resveratrol on the expression of <i>catalase, glutathione peroxidase</i>, and <i>superoxide dismutase</i> genes in the ovary and their activity in the serum of rats exposed to lead acetate: An experimental study.","authors":"Mohammad Karimian, Mozhdeh Ghadiri, Seyedeh Mahsa Poormoosavi, Hossein Najafzadehvarzi","doi":"10.18502/ijrm.v22i11.17821","DOIUrl":"10.18502/ijrm.v22i11.17821","url":null,"abstract":"<p><strong>Background: </strong>Lead (Pb) could be toxic to the female reproductive system, and resveratrol (Res) may overcome this toxicity.</p><p><strong>Objective: </strong>To investigate the Res impact on the catalase (<i>Cat</i>), glutathione peroxidase (<i>Gpx</i>), and superoxide dismutase (<i>Sod</i>) gene expression in the ovary and on the Cat and Gpx enzyme activity in the serum of rats exposed to lead acetate.</p><p><strong>Materials and methods: </strong>In this experimental study, 33 female Wistar rats (8-10 wk, 180-200 gr) were divided into 6 groups: a control group (normal saline), a Res group (40 mg/kg), and a Pb group (lead acetate 30 mg/kg). 3 additional groups received lead acetate (30 mg/kg) with Res at 20, 40, and 80 mg/kg for 21 days. Gene expression of <i>Cat</i>, <i>Gpx</i>, and <i>Sod</i> was measured via qPCR, and serum Cat and Gpx activity was assessed using standard methods. Bioinformatics tools were used to evaluate Res effects on gene and protein function.</p><p><strong>Results: </strong>Lead acetate significantly downregulates <i>Cat</i>, <i>Gpx</i>, and <i>Sod</i> gene expression, but Res significantly upregulates gene expression, especially at doses of 40 mg/kg for <i>Cat</i>, 20 mg/kg and 40 mg/kg for <i>Gpx</i>, and 80 mg/kg for <i>Sod</i>. Cat and Gpx enzyme activity increased and decreased in the lead acetate group, respectively. However, Res in all doses decreased only the Cat enzyme activity. Bioinformatics analysis indicates that Res can interact with the promoter regions and cavities of all 3 enzymes.</p><p><strong>Conclusion: </strong>Pb can dysregulate the expression and activity of the studied enzymes. However, the impact of Res is influenced by the dose, with 40 mg/kg frequently being the most effective.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"22 11","pages":"883-894"},"PeriodicalIF":1.6,"publicationDate":"2024-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11757674/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143046490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The association between the amount of fetal fraction in cell-free DNA testing and adverse pregnancy outcomes: A cohort study.","authors":"Maryam Aryavand, Maryam Nurzadeh, Marjan Ghaemi, Sina Eskandari Delfan, Vajiheh Marsoosi","doi":"10.18502/ijrm.v22i11.17824","DOIUrl":"10.18502/ijrm.v22i11.17824","url":null,"abstract":"<p><strong>Background: </strong>Noninvasive perinatal testing is a new method of screening for aneuploidy called cell-free DNA (cfDNA). Fetal fraction (FF) plays a crucial role in assessing the reliability of aneuploidy detection through noninvasive perinatal testing.</p><p><strong>Objective: </strong>We aimed to investigate the association between the amount of FF in cfDNA testing and adverse pregnancy outcomes.</p><p><strong>Materials and methods: </strong>This cohort study was conducted on 619 singleton pregnant women who were candidates for cfDNA testing and were referred to the perinatology clinics of Shariati hospital and Arash Women's hospital, both affiliated with Tehran University of Medical Sciences, Tehran, Iran from March 2019 to June 2020. The FF was extracted from the cfDNA test results, and the participants were followed until delivery.</p><p><strong>Results: </strong>A total of 619 singleton pregnant women with a mean <math><mo>±</mo></math> SD age and FF of 34.4 <math><mo>±</mo></math> 4.85 and 8.39 <math><mo>±</mo></math> 3.95, respectively, participated in the study. A significant association between maternal age and FF was not found (p = 0.12). A lower FF was associated with a rise in the incidence of gestational diabetes mellitus (p = 0.02) and a higher FF was associated with a rise in the incidence of fetal growth restriction (p <math><mo><</mo></math> 0.001). However, high or low FF was not associated with pre-eclampsia, premature rupture of membranes, birth weight, or delivery time. No significant association was found between FF and multiple of the median of pregnancy-associated plasma protein-A and free β-human chorionic gonadotropin.</p><p><strong>Conclusion: </strong>The amount of FF may be considered a predictor of certain adverse pregnancy outcomes. Therefore, maternity care should be performed more carefully for women with high or low FF.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"22 11","pages":"919-926"},"PeriodicalIF":1.6,"publicationDate":"2024-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11757670/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143046504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The relationship between maternal vitamin D levels and osteopenia development in preterm infants: A cross-sectional study.","authors":"Razieh Sadat Tabatabaei, Seyed Reza Mirjalili, Atefeh Ashrafi, Farimah Shamsi","doi":"10.18502/ijrm.v22i11.17818","DOIUrl":"10.18502/ijrm.v22i11.17818","url":null,"abstract":"<p><strong>Background: </strong>Osteopenia of prematurity (OP) is characterized by reduced bone mineral content, and vitamin D deficiency may worsen OP by affecting bone metabolism.</p><p><strong>Objective: </strong>This study aimed to investigate the correlation between maternal vitamin D levels and biochemical markers related to OP.</p><p><strong>Materials and methods: </strong>This analytical cross-sectional study, conducted at Shahid Sadoughi hospital, Yazd, Iran, from June 2022 to September 2023, included 49 pregnant women and their preterm infants. Based on the serum alkaline phosphatase and phosphorus levels of the infants at birth and 4 wk after birth, they were divided into osteopenic and non-osteopenic groups. Maternal and neonatal vitamin D and calcium levels were then compared between these groups.</p><p><strong>Results: </strong>The mean maternal vitamin D level was 24.9 <math><mo>±</mo></math> 15.09 ng/ml, with 36% showing deficiency. 11 neonates at 4 wk exhibited osteopenia based on phosphorus and serum alkaline phosphatase levels. Those with osteopenia had significantly lower vitamin D (p = 0.032) and calcium levels compared to non-osteopenic neonates (p = 0.043), although maternal vitamin D mean was not a significant risk factor for OP (p = 0.313).</p><p><strong>Conclusion: </strong>The results suggest that maternal vitamin D levels do not have a significant association with the incidence of osteopenia in neonates, as the mean maternal vitamin D concentration was not identified as a risk factor for this condition. Therefore, it is recommended that future research should investigate alternative factors that may contribute to the development of osteopenia in preterm infants.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"22 11","pages":"845-856"},"PeriodicalIF":1.6,"publicationDate":"2024-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11757673/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143046510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of utilization of amplified blastocoel fluid DNA gel electrophoresis band intensity as an additional minimally invasive approach in embryo selection: A cross-sectional study.","authors":"Fattaneh Khajehoseini, Zahra Noormohammadi, Poopak Eftekhari-Yazdi, Hamid Gourabi, Reza Pazhoomand, Shirzad Hosseinishenatal, Masood Bazrgar","doi":"10.18502/ijrm.v22i11.17823","DOIUrl":"10.18502/ijrm.v22i11.17823","url":null,"abstract":"<p><strong>Background: </strong>Embryo selection for transfer is critical in assisted reproduction. The presence of DNA in the blastocoel cavity of human blastocysts is assumed to be a consequence of common preimplantation chromosomal abnormalities.</p><p><strong>Objective: </strong>This study examined the relationship between the amount of blastocoel fluid (BF) DNA and the band intensity of amplified BF-DNA in gel electrophoresis, considering the influence of ploidy status.</p><p><strong>Materials and methods: </strong>This cross-sectional study categorizes blastocysts into 2 groups based on the array comparative genomic hybridization results by trophectoderm biopsy -the euploid and aneuploid group. After morphological scoring, a biopsy of BF was performed for whole genome amplification, followed by an assessment of band intensity and BF-DNA quantification. The relationship between BF-DNA levels, band intensity, and ploidy status were analyzed.</p><p><strong>Results: </strong>The level of BF-DNA was higher in the aneuploid group compared to the euploid group, but the difference was not statistically significant (p = 0.2). We observed that the band intensity was affected by the ploidy status of the embryos, although this difference was not statistically significant (p = 0.059). Notably, electrophoresis band of all embryos with chromosomal loss was strong. No correlation was observed between embryo morphology and chromosomal ploidy (p = 0.8).</p><p><strong>Conclusion: </strong>Our findings indicate that BF-DNA band intensity on agarose gel is not currently applicable for preimplantation embryo selection. It seems that embryos with chromosomal loss are more prone to DNA release to BF. The disrelation between embryo morphology and ploidy status represents the necessity of minimally invasive embryo screening methods based on ploidy status.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"22 11","pages":"907-918"},"PeriodicalIF":1.6,"publicationDate":"2024-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11757668/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143046134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}