褪黑素和左旋肉碱联合使用对小鼠卵母细胞体外成熟的影响:一项实验研究

IF 1.6 Q3 OBSTETRICS & GYNECOLOGY
International Journal of Reproductive Biomedicine Pub Date : 2024-09-12 eCollection Date: 2024-07-01 DOI:10.18502/ijrm.v22i7.16961
Raziye Chegini, Morteza Sadeghi, Sadegh Shirian, Fatemeh Sabbaghziarani, Ehsan Aali, Pouriya Soleimani, Mohammad Reza Ashtari Majelan, Fariba Zafari, Shahram Darabi
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引用次数: 0

摘要

背景:褪黑素和左旋肉碱是自由基清除剂,具有抗凋亡和抗氧化特性,可改善卵母细胞发育:本研究旨在寻找褪黑素和左旋肉碱这两种抗氧化剂在小鼠模型中对卵母细胞形态、成熟、凋亡以及骨形态发生蛋白15(BMP-15)和生长分化因子9(GDF-9)基因表达的可能影响:使用母马血清促性腺激素对 60 只雌性 NMRI 小鼠进行腹腔注射,以达到过度刺激的目的。注射后第 2 天,从每只小鼠体内收集 70 个积液-卵母细胞复合体。然后将收集的卵母细胞随机分为 4 组,包括对照组、褪黑素组、左旋肉碱组和褪黑素 + 左旋肉碱组。使用光学显微镜评估卵母细胞的形态和成熟率。用末端脱氧核苷酸转移酶介导的 dUTP 缺口标记法鉴定细胞凋亡,并用实时聚合酶链反应评估 BMP-15 和生长与分化因子 GDF-9 基因的表达:结果:与其他组相比,左旋肉碱和褪黑素联合治疗组的卵母细胞直径明显增大(P 0.05)。左旋肉碱组的卵母细胞细胞质形态平均百分比最高。末端脱氧核苷酸转移酶介导的 dUTP 缺口标记结果表明,褪黑素+左旋肉碱组的细胞凋亡率最低。此外,组合组的卵母细胞数量和成熟率最高。与对照组相比,所有治疗组的BMP-15和GDF-9基因均明显上调:我们的研究结果表明,褪黑素+左旋肉碱联合用药是体外促进卵母细胞成熟的更有效选择。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effects of combination of melatonin and L-carnitine on in vitro maturation in mouse oocytes: An experimental study.

Background: Melatonin and L-carnitine are free radical scavengers with antiapoptotic and antioxidant properties that improve oocyte development.

Objective: This study aimed to find the possible effect of combining 2 antioxidant agents of melatonin and L-carnitine on oocyte morphology, maturation, apoptosis, and expression of bone morphogenetic protein 15 (BMP-15) and growth differentiation factor 9 (GDF-9) genes in a mice model.

Materials and methods: To overstimulation, 60 female NMRI mice were injected intraperitoneally using mare serum gonadotropin. On day 2 post-injection, 70 cumulus-oocyte complexes were collected from each mouse. The collected oocytes randomly were then divided into 4 groups including, the control, melatonin, L-carnitine, and melatonin + L-carnitine groups. The morphology and maturation rate of the oocytes was evaluated using a light microscope. Apoptosis was identified by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling assay and the expression of BMP-15 and growth and differentiation factor GDF-9 genes was also evaluated by real-time polymerase chain reaction.

Results: Oocyte diameter significantly was increased in combination treatment of L-carnitine and melatonin compared to other groups (p < 0.05). L-carnitine group showed the highest mean percentage of oocyte cytoplasmic pattern. Results of the terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling indicated that the lowest apoptosis rate belonged to the melatonin + L-carnitine group. Moreover, the combination groups showed the highest number of oocytes and maturation rate. The BMP-15 and GDF-9 genes were significantly upregulated in all treatment groups compared to the control group.

Conclusion: Our results suggested a combination of melatonin + L-carnitine administration as a more effective choice for in vitro promotion of oocyte maturation.

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来源期刊
CiteScore
2.40
自引率
7.70%
发文量
93
审稿时长
16 weeks
期刊介绍: The International Journal of Reproductive BioMedicine (IJRM), formerly published as "Iranian Journal of Reproductive Medicine (ISSN: 1680-6433)", is an international monthly scientific journal for who treat and investigate problems of infertility and human reproductive disorders. This journal accepts Original Papers, Review Articles, Short Communications, Case Reports, Photo Clinics, and Letters to the Editor in the fields of fertility and infertility, ethical and social issues of assisted reproductive technologies, cellular and molecular biology of reproduction including the development of gametes and early embryos, assisted reproductive technologies in model system and in a clinical environment, reproductive endocrinology, andrology, epidemiology, pathology, genetics, oncology, surgery, psychology, and physiology. Emerging topics including cloning and stem cells are encouraged.
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