International Journal of Reproductive Biomedicine最新文献

{"title":"Protective effects of resveratrol on the expression of <i>catalase, glutathione peroxidase</i>, and <i>superoxide dismutase</i> genes in the ovary and their activity in the serum of rats exposed to lead acetate: An experimental study.","authors":"Mohammad Karimian, Mozhdeh Ghadiri, Seyedeh Mahsa Poormoosavi, Hossein Najafzadehvarzi","doi":"10.18502/ijrm.v22i11.17821","DOIUrl":"10.18502/ijrm.v22i11.17821","url":null,"abstract":"<p><strong>Background: </strong>Lead (Pb) could be toxic to the female reproductive system, and resveratrol (Res) may overcome this toxicity.</p><p><strong>Objective: </strong>To investigate the Res impact on the catalase (<i>Cat</i>), glutathione peroxidase (<i>Gpx</i>), and superoxide dismutase (<i>Sod</i>) gene expression in the ovary and on the Cat and Gpx enzyme activity in the serum of rats exposed to lead acetate.</p><p><strong>Materials and methods: </strong>In this experimental study, 33 female Wistar rats (8-10 wk, 180-200 gr) were divided into 6 groups: a control group (normal saline), a Res group (40 mg/kg), and a Pb group (lead acetate 30 mg/kg). 3 additional groups received lead acetate (30 mg/kg) with Res at 20, 40, and 80 mg/kg for 21 days. Gene expression of <i>Cat</i>, <i>Gpx</i>, and <i>Sod</i> was measured via qPCR, and serum Cat and Gpx activity was assessed using standard methods. Bioinformatics tools were used to evaluate Res effects on gene and protein function.</p><p><strong>Results: </strong>Lead acetate significantly downregulates <i>Cat</i>, <i>Gpx</i>, and <i>Sod</i> gene expression, but Res significantly upregulates gene expression, especially at doses of 40 mg/kg for <i>Cat</i>, 20 mg/kg and 40 mg/kg for <i>Gpx</i>, and 80 mg/kg for <i>Sod</i>. Cat and Gpx enzyme activity increased and decreased in the lead acetate group, respectively. However, Res in all doses decreased only the Cat enzyme activity. Bioinformatics analysis indicates that Res can interact with the promoter regions and cavities of all 3 enzymes.</p><p><strong>Conclusion: </strong>Pb can dysregulate the expression and activity of the studied enzymes. However, the impact of Res is influenced by the dose, with 40 mg/kg frequently being the most effective.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"22 11","pages":"883-894"},"PeriodicalIF":1.6,"publicationDate":"2024-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11757674/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143046490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The association between the amount of fetal fraction in cell-free DNA testing and adverse pregnancy outcomes: A cohort study.","authors":"Maryam Aryavand, Maryam Nurzadeh, Marjan Ghaemi, Sina Eskandari Delfan, Vajiheh Marsoosi","doi":"10.18502/ijrm.v22i11.17824","DOIUrl":"10.18502/ijrm.v22i11.17824","url":null,"abstract":"<p><strong>Background: </strong>Noninvasive perinatal testing is a new method of screening for aneuploidy called cell-free DNA (cfDNA). Fetal fraction (FF) plays a crucial role in assessing the reliability of aneuploidy detection through noninvasive perinatal testing.</p><p><strong>Objective: </strong>We aimed to investigate the association between the amount of FF in cfDNA testing and adverse pregnancy outcomes.</p><p><strong>Materials and methods: </strong>This cohort study was conducted on 619 singleton pregnant women who were candidates for cfDNA testing and were referred to the perinatology clinics of Shariati hospital and Arash Women's hospital, both affiliated with Tehran University of Medical Sciences, Tehran, Iran from March 2019 to June 2020. The FF was extracted from the cfDNA test results, and the participants were followed until delivery.</p><p><strong>Results: </strong>A total of 619 singleton pregnant women with a mean <math><mo>±</mo></math> SD age and FF of 34.4 <math><mo>±</mo></math> 4.85 and 8.39 <math><mo>±</mo></math> 3.95, respectively, participated in the study. A significant association between maternal age and FF was not found (p = 0.12). A lower FF was associated with a rise in the incidence of gestational diabetes mellitus (p = 0.02) and a higher FF was associated with a rise in the incidence of fetal growth restriction (p <math><mo><</mo></math> 0.001). However, high or low FF was not associated with pre-eclampsia, premature rupture of membranes, birth weight, or delivery time. No significant association was found between FF and multiple of the median of pregnancy-associated plasma protein-A and free β-human chorionic gonadotropin.</p><p><strong>Conclusion: </strong>The amount of FF may be considered a predictor of certain adverse pregnancy outcomes. Therefore, maternity care should be performed more carefully for women with high or low FF.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"22 11","pages":"919-926"},"PeriodicalIF":1.6,"publicationDate":"2024-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11757670/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143046504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The relationship between maternal vitamin D levels and osteopenia development in preterm infants: A cross-sectional study.","authors":"Razieh Sadat Tabatabaei, Seyed Reza Mirjalili, Atefeh Ashrafi, Farimah Shamsi","doi":"10.18502/ijrm.v22i11.17818","DOIUrl":"10.18502/ijrm.v22i11.17818","url":null,"abstract":"<p><strong>Background: </strong>Osteopenia of prematurity (OP) is characterized by reduced bone mineral content, and vitamin D deficiency may worsen OP by affecting bone metabolism.</p><p><strong>Objective: </strong>This study aimed to investigate the correlation between maternal vitamin D levels and biochemical markers related to OP.</p><p><strong>Materials and methods: </strong>This analytical cross-sectional study, conducted at Shahid Sadoughi hospital, Yazd, Iran, from June 2022 to September 2023, included 49 pregnant women and their preterm infants. Based on the serum alkaline phosphatase and phosphorus levels of the infants at birth and 4 wk after birth, they were divided into osteopenic and non-osteopenic groups. Maternal and neonatal vitamin D and calcium levels were then compared between these groups.</p><p><strong>Results: </strong>The mean maternal vitamin D level was 24.9 <math><mo>±</mo></math> 15.09 ng/ml, with 36% showing deficiency. 11 neonates at 4 wk exhibited osteopenia based on phosphorus and serum alkaline phosphatase levels. Those with osteopenia had significantly lower vitamin D (p = 0.032) and calcium levels compared to non-osteopenic neonates (p = 0.043), although maternal vitamin D mean was not a significant risk factor for OP (p = 0.313).</p><p><strong>Conclusion: </strong>The results suggest that maternal vitamin D levels do not have a significant association with the incidence of osteopenia in neonates, as the mean maternal vitamin D concentration was not identified as a risk factor for this condition. Therefore, it is recommended that future research should investigate alternative factors that may contribute to the development of osteopenia in preterm infants.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"22 11","pages":"845-856"},"PeriodicalIF":1.6,"publicationDate":"2024-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11757673/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143046510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of utilization of amplified blastocoel fluid DNA gel electrophoresis band intensity as an additional minimally invasive approach in embryo selection: A cross-sectional study.","authors":"Fattaneh Khajehoseini, Zahra Noormohammadi, Poopak Eftekhari-Yazdi, Hamid Gourabi, Reza Pazhoomand, Shirzad Hosseinishenatal, Masood Bazrgar","doi":"10.18502/ijrm.v22i11.17823","DOIUrl":"10.18502/ijrm.v22i11.17823","url":null,"abstract":"<p><strong>Background: </strong>Embryo selection for transfer is critical in assisted reproduction. The presence of DNA in the blastocoel cavity of human blastocysts is assumed to be a consequence of common preimplantation chromosomal abnormalities.</p><p><strong>Objective: </strong>This study examined the relationship between the amount of blastocoel fluid (BF) DNA and the band intensity of amplified BF-DNA in gel electrophoresis, considering the influence of ploidy status.</p><p><strong>Materials and methods: </strong>This cross-sectional study categorizes blastocysts into 2 groups based on the array comparative genomic hybridization results by trophectoderm biopsy -the euploid and aneuploid group. After morphological scoring, a biopsy of BF was performed for whole genome amplification, followed by an assessment of band intensity and BF-DNA quantification. The relationship between BF-DNA levels, band intensity, and ploidy status were analyzed.</p><p><strong>Results: </strong>The level of BF-DNA was higher in the aneuploid group compared to the euploid group, but the difference was not statistically significant (p = 0.2). We observed that the band intensity was affected by the ploidy status of the embryos, although this difference was not statistically significant (p = 0.059). Notably, electrophoresis band of all embryos with chromosomal loss was strong. No correlation was observed between embryo morphology and chromosomal ploidy (p = 0.8).</p><p><strong>Conclusion: </strong>Our findings indicate that BF-DNA band intensity on agarose gel is not currently applicable for preimplantation embryo selection. It seems that embryos with chromosomal loss are more prone to DNA release to BF. The disrelation between embryo morphology and ploidy status represents the necessity of minimally invasive embryo screening methods based on ploidy status.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"22 11","pages":"907-918"},"PeriodicalIF":1.6,"publicationDate":"2024-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11757668/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143046134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vimentin as a contributing factor in SARS-CoV-2-induced orchitis on postmortem testicular autopsy of COVID-19 cases: A case-control study.","authors":"Soheila Akaberi-Nasrabadi, Azam Sabbaghi, Behzad M Toosi, Parsa Ghorbanifaraz, Gholam-Reza Mahmoudiasl, Abbas Aliaghaei, Hajarsadat Faghihi Hosseinabadi, Shahrokh Paktinat, Mohammad-Amin Abdollahifar","doi":"10.18502/ijrm.v22i11.17822","DOIUrl":"10.18502/ijrm.v22i11.17822","url":null,"abstract":"<p><strong>Background: </strong>Coronavirus disease 2019 (COVID-19) was identified in China in late December 2019 and led to a pandemic that resulted in millions of confirmed cases and deaths. The causative agent, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), uses distinct receptors and co-receptors to enter host cells. Vimentin has emerged as a potential co-receptor for SARS-CoV-2 due to the high level of vimentin expression in testis tissue.</p><p><strong>Objective: </strong>The present study investigated the link between vimentin expression level and SARS-CoV-2-induced orchitis.</p><p><strong>Materials and methods: </strong>In this case-control study, testis autopsy samples were collected immediately after the death of both COVID-19 cases and a control group that included individuals who died due to accidental causes. Gene expression and immunohistochemical assays were conducted to evaluate the level of vimentin expression, cell proliferation, and leukocyte infiltration.</p><p><strong>Results: </strong>A significant expression of vimentin and infiltration of immune cells (CD68+, CD38+, and CD138+) in the testicular tissue of COVID-19 cases, along with extensive immunoglobulin G precipitation and reduced inhibin expression (p = 0.001) were observed. Additionally, gene expression analysis revealed increased expression of vimentin and decreased expression of the proliferation markers Ki67 and proliferating cell nuclear antigen, suggesting that SARS-CoV-2 may disrupt spermatogenesis through immune responses and the arrest of cell proliferation.</p><p><strong>Conclusion: </strong>There may be a strong link between vimentin expression and COVID-19-induced orchitis. Further studies are needed to confirm these findings. Considering some limitations, vimentin can be used as a biomarker option for testicular damage following COVID-19-induced orchitis.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"22 11","pages":"895-906"},"PeriodicalIF":1.6,"publicationDate":"2024-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11757669/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143046529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro fertilization pregnancy may cause fetal thymic volume involution: A case-control study.","authors":"Nurgul Selin Kaya, Emine Seda Guvendag Guven, Suleyman Guven","doi":"10.18502/ijrm.v21i10.14537","DOIUrl":"https://doi.org/10.18502/ijrm.v21i10.14537","url":null,"abstract":"<p><strong>Background: </strong>The effect of modern infertility treatment modalities on fetal thymic volume has not been well known.</p><p><strong>Objective: </strong>3-dimensional (3D) fetal thymus volumes of 18-24 wk in vitro fertilization (IVF) pregnancies and spontaneous pregnancy cases were compared.</p><p><strong>Materials and methods: </strong>135 cases were evaluated in this prospective case-control study. The study was conducted between July 2019 and July 2020 at a university hospital in Trabzon, Turkey. Fetal thymus volume was calculated in the pregnant cases included in the study with the help of the virtual organ computer-assisted analysis system included in the advanced ultrasonography system. The fetal thymus volumes were compared between pregnant women with IVF and spontaneous pregnant women.</p><p><strong>Results: </strong>The fetal thymus size was significantly lower in the IVF pregnancy group than in spontaneous pregnancy cases (p <math><mo><</mo></math> 0.001). It was found that the fetal complications, such as non-reassuring fetal health status and requirement for neonatal intensive care, were higher in cases who became pregnant after IVF treatment. It was also found that the rate of any pregnancy complication was significantly higher in IVF pregnancy group (p = 0.02).</p><p><strong>Conclusion: </strong>In light of these results, it may be concluded that small fetal thymus size may be another fetal complication of IVF pregnancies.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"21 10","pages":"819-826"},"PeriodicalIF":1.3,"publicationDate":"2023-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10698353/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138803983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Renal artery Doppler in fetal sonography: A narrative review.","authors":"Ashraf Sadat Jamal, Mahdieh Modarresi","doi":"10.18502/ijrm.v21i10.14534","DOIUrl":"https://doi.org/10.18502/ijrm.v21i10.14534","url":null,"abstract":"<p><p>Doppler imaging is a non-invasive method in evaluating fetal circulation. Renal artery doppler (RAD) has been used for assessing fetal well-being in several studies. The aim of this narrative review was to accumulate and classify current evidence on RAD in fetal sonography. Articles until November 2022 were searched. After removing ineligible articles, 51 studies were included. Present articles were about RAD assessment in cases with amniotic fluid level changes, fetal growth restriction, fetal renal diseases, monochorionic twin pregnancies, preeclampsia, and gestational diabetes mellitus. The complex physiology of fetal kidney function may explain different results observed in different studies about the role of RAD in fetal assessment. It seems this factor can be useful in assessing some groups like diabetic pregnant women, and it should be used accompanying other related factors like kidney size. Further research is needed to evaluate the effectiveness of RAD in clinical management.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"21 10","pages":"789-800"},"PeriodicalIF":1.3,"publicationDate":"2023-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10698356/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138803985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expressions of vascular endothelial growth factor A, mucin-1, colony-stimulating factor-1, heparin-binding epidermal growth factor-like growth factor, and fibroblast growth factor 2 genes in the female reproductive tracts of women with ectopic pregnancy: A case-control study.","authors":"Sima Golkar, Zahra Chekini, Fatemehsadat Amjadi, Parvaneh Afsharian, Aida Najafian, Firouzeh Ghaffari, Reza Aflatoonian","doi":"10.18502/ijrm.v21i10.14535","DOIUrl":"https://doi.org/10.18502/ijrm.v21i10.14535","url":null,"abstract":"<p><strong>Background: </strong>Ectopic pregnancy (EP) is defined as embryo implantation in a location other than the uterine cavity.</p><p><strong>Objective: </strong>We aimed to evaluate the expression of several genes, which may play a role in EP, in the ampulla region of fallopian tubes and endometrial tissue of women with EP.</p><p><strong>Materials and methods: </strong>In this case-control study, 5 women who underwent salpingectomy due to EP, comprised the 5 pseudo-pregnant women as a control group. These participants referred to the Royan Institute, Shariati, and Arash hospital, Tehran, Iran during 2019-2021. We evaluated the expressions of vascular endothelial growth factor A<i>,</i> mucin-1<i>,</i> colony-stimulating factor-1<i>,</i> heparin-binding epidermal growth factor-like growth factor <i>(HBEGF),</i> and fibroblast growth factor 2 genes in the fallopian tube and endometrium of EP cases by real-time polymerase chain reaction using specific primers.</p><p><strong>Results: </strong>The vascular endothelial growth factor expression was significantly higher in the ampulla region of the controls. However, no significant differences were observed in endometrial tissue. Assessments of colony-stimulating factor-1 and fibroblast growth factor 2 showed no significant differences between the case and control groups. <i>HBEGF</i> showed significantly higher expression in the ampulla region of EP cases, but no significant difference was observed in <i>HBEGF</i> expression in the endometrial tissues of the study groups. Mucin-1expression was significantly higher in both study regions of the EP cases.</p><p><strong>Conclusion: </strong>Our results have strongly suggested that these genes play important roles in proper implantation, and disruptions in their expression patterns could lead to EP. However, more studies are needed to confirm the current findings.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"21 10","pages":"801-808"},"PeriodicalIF":1.3,"publicationDate":"2023-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10698352/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138803866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of druggable hub genes and key pathways associated with cervical cancer by protein-protein interaction analysis: An in silico study.","authors":"Azizeh Asadzadeh, Nafiseh Ghorbani, Katayoun Dastan","doi":"10.18502/ijrm.v21i10.14536","DOIUrl":"https://doi.org/10.18502/ijrm.v21i10.14536","url":null,"abstract":"<p><strong>Background: </strong>The uncontrolled growth of abnormal cells in the cervix leads to cervical cancer (CC), the fourth most common gynecologic cancer. So far, many studies have been conducted on CC; however, it is still necessary to discover the hub gene, key pathways, and the exact underlying mechanisms involved in developing this disease.</p><p><strong>Objective: </strong>This study aims to use gene expression patterns and protein-protein interaction (PPI) network analysis to identify key pathways and druggable hub genes in CC.</p><p><strong>Materials and methods: </strong>In this in silico analysis, 2 microarray gene expression datasets; GSE63514 (104 cancer and 24 normal samples), and GSE9750 (42 cancer and 24 normal samples) were extracted from gene expression omnibus to identify common differentially expressed genes between them. Gene ontology and Kyoto encyclopedia of genes and genomes pathway analysis were performed via the Enrichr database. STRING 12.0 database and CytoHubba plugin in Cytoscape 3.9.1 software were implemented to create and analyze the PPI network. Finally, druggable hub genes were screened.</p><p><strong>Results: </strong>Based on the degree method, 10 key genes were known as the hub genes after the screening of PPI networks by the CytoHubba plugin. <i>NCAPG</i>, <i>KIF11</i>, <i>TTK</i>, <i>PBK</i>, <i>MELK</i>, <i>ASPM</i>, <i>TPX2</i>, <i>BUB1</i>, <i>TOP2A</i>, and <i>KIF2C</i> are the key genes, of which 5 genes (<i>KIF11</i>, <i>TTK</i>, <i>PBK</i>, <i>MELK</i>, and <i>TOP2A</i>) were druggable.</p><p><strong>Conclusion: </strong>This research provides a novel vision for designing therapeutic targets in patients with CC. However, these findings should be verified through additional experiments.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"21 10","pages":"809-818"},"PeriodicalIF":1.3,"publicationDate":"2023-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10698354/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138803980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of proprotein convertase subtilisin/kexin type 9 protein and oxidative stress indicators in women with preeclampsia: A case-control study.","authors":"Anahita Abbasifard, Shohreh Alimohammadi, Ebrahim Abbasi Oshaghi, Gholamreza Shafiee","doi":"10.18502/ijrm.v21i10.14540","DOIUrl":"https://doi.org/10.18502/ijrm.v21i10.14540","url":null,"abstract":"<p><strong>Background: </strong>Proprotein convertase subtilisin/kexin type 9 (PCSK9) protein is one of the factors associated with oxidative stress and dyslipidemia disorders.</p><p><strong>Objective: </strong>This study aimed to evaluate the lipid profile, PCSK9 levels, and oxidative stress in preeclampsia.</p><p><strong>Materials and methods: </strong>This case-control study was conducted at Sina hospital in Hamadan University of Medical Sciences, Hamadan, Iran from August 2020-May 2021. The average maternal age of included participants was 30 yr with 30 preeclampsia and 30 healthy pregnant women. After clinical examination, the fasting blood samples were collected, and the serum PCSK9 protein concentration, superoxide dismutase, glutathione peroxidase activities, and glutathione levels were determined by enzyme-linked immunosorbent assay. Total antioxidant capacity, total oxidant status, and malondialdehyde levels were determined manually.</p><p><strong>Results: </strong>The average maternal age of participants were 29.97 <math><mo>±</mo></math> 4.75 and 31.23 <math><mo>±</mo></math> 5.85 yr, respectively. The concentrations of total cholesterol, low-density lipoprotein cholesterol (LDL-C), PCSK9, total antioxidant capacity, and malondialdehyde levels were higher in the preeclampsia group compared with control (p <math><mo><</mo></math> 0.02). Total oxidant status, glutathione levels, superoxide dismutase, glutathione peroxidase activities were lower in the cases group compared with the control group (p <math><mo><</mo></math> 0.01). The PCSK9 variable had a significant negative association with antioxidant parameters; however, a significant positive association was observed between PCSK9 level and parameters of LDL-C.</p><p><strong>Conclusion: </strong>PCSK9 is associated with increased serum levels of LDL-C and oxidative factors in pregnant women that increase the risk of endothelial damage and hypertension in preeclampsia.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"21 10","pages":"845-852"},"PeriodicalIF":1.3,"publicationDate":"2023-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10698358/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138803825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}