International Journal of Reproductive Biomedicine最新文献

{"title":"Sexual function and sexual satisfaction in individuals undergoing infertility: A systematic review.","authors":"Armin Firoozi, Farah Lotfi Kashani, Shahram Vaziri","doi":"10.18502/ijrm.v21i9.14404","DOIUrl":"10.18502/ijrm.v21i9.14404","url":null,"abstract":"<p><strong>Background: </strong>Although sexual function (SF) and sexual satisfaction (SS) are the essential factors influenced negatively by infertility and may be associated with delaying or disrupting infertility treatment, no systematic review has assessed the results of these studies so far.</p><p><strong>Objective: </strong>The present study aimed to systematically review published interventional research regarding SF and SS among infertile individuals.</p><p><strong>Materials and methods: </strong>In this systematic review, the databases of PubMed, MEDLINE, Web of Science, Scopus, ScienceDirect, Cochrane Library, PsycInfo, Google Scholar, and SID were searched to retrieve the relevant studies in Persian and English languages up to August 2021.</p><p><strong>Results: </strong>23 interventional studies were included in this systematic review, of which only 2 were low quality based on the critical appraisal skills program checklist. The interventions of the included studies were classified into 2 main categories: educational and psychological interventions for increasing the SF and SS among infertile women or couples.</p><p><strong>Conclusion: </strong>There was no clear evidence to understand the most effective method for increasing SF and satisfaction among couples with infertility. Based on the results of the included studies, all of them showed the effectiveness of the various interventions performed on sexual health dimensions among couples with infertility. This systematic review showed that most SF studies were educational, whereas the SS were more psychologically interventional. Conducting standardized and high-quality randomized controlled trials focusing on SF and SS is recommended.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"21 9","pages":"697-714"},"PeriodicalIF":1.6,"publicationDate":"2023-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10643682/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134648944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent advances in developing 3D culture systems of spermatogonial stem cell preservation and differentiation: A narrative review.","authors":"Leyla Fath-Bayati, Leila Naserpour, Mohadeseh Khoshandam, Rahil Jannatifar, Hoda Fazaeli","doi":"10.18502/ijrm.v21i9.14397","DOIUrl":"https://doi.org/10.18502/ijrm.v21i9.14397","url":null,"abstract":"<p><p>Male infertility has received vast attention in recent years and has no clear etiology in almost 40% of cases. Several methods have been suggested for preserving sperm and spermatogonial stem cells (SSCs) in both in vivo and in vitro conditions. The efficacy of these methods is related to their abilities, including providing an optimal environment for sperm preservation and long-term SSC culture for in vivo and in vitro differentiation of these cells. In this review article, a full MEDLINE/PubMed search was performed using the following search terms: \"Spermatogonial Progenitor Cells, Stem Cells, Fertility Preservations, Sperm Freezing, Cell Differentiations, Tissue Scaffold, 3-Dimensional Cell Culture\", which retrieved results from 1973-2022. Related articles were added to the bibliography of selected articles. Exclusion criteria included non-English language, abstract only, and unrelated articles. The production of functioning male germ cells is suggested by introducing modern bioengineered systems as a new hope for the maintenance of male fertility. Till now, few in vitro spermatogenesis investigations have provided appreciable amounts of mature gametes. Each method had benefits and disadvantages, but the 3-dimensional culture method had the greatest impact on the differentiation and preservation of SSCs. One of the critical elements of research is the preservation of sperm and the differentiation of SSCs. Several methods have been employed in this area. Various scaffolds providing an environment similar to an extracellular matrix and conditions for germ cell development and survival have been employed in recent research.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"21 9","pages":"681-696"},"PeriodicalIF":1.3,"publicationDate":"2023-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10643686/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134648943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Maternal and perinatal outcomes in pregnant women with COVID-19 in a referral academic center in Iran.","authors":"Paria Boustani, Laleh Eslamian, Ashraf Aleyasin, Ashraf Jamal, Nasim Eshraghi, Marjan Ghaemi","doi":"10.18502/ijrm.v21i9.14403","DOIUrl":"https://doi.org/10.18502/ijrm.v21i9.14403","url":null,"abstract":"The Article Abstract is not available.","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"21 9","pages":"767-769"},"PeriodicalIF":1.3,"publicationDate":"2023-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10643680/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134648941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pregnancy outcome in long- versus short-acting gonadotropin-releasing hormone agonist cycles in participants with normal ovarian reserve: An RCT.","authors":"Roya Kabodmehri, Nasrin Ghanami Gashti, Azadeh Raoufi, Marzieh Mehrafza, Zahra Nikpouri, Elmira Hosseinzadeh, Ahmad Hosseini","doi":"10.18502/ijrm.v21i9.14402","DOIUrl":"https://doi.org/10.18502/ijrm.v21i9.14402","url":null,"abstract":"<p><strong>Background: </strong>There is no agreement on which of the 2 gonadotropin-releasing hormone (GnRH) agonist protocols are the most efficient, neither there is any consensus on which one yields a better clinical pregnancy percentage.</p><p><strong>Objective: </strong>The present study aims to compare the effectiveness of reduced dosages of long- and short-acting GnRH agonists on pregnancy outcomes.</p><p><strong>Materials and methods: </strong>In this randomized controlled clinical trial, 400 women were randomly assigned to 2 groups (n = 200/group): the reduced dosage of long-acting GnRH agonist group (group 1, 1.25 mg Decapeptyl) and the short-acting GnRH agonist group (group 2, 0.5 mg/day Buserelin Acetate). The study was conducted at Mehr Medical Institute, Rasht, Iran between July 2019 and July 2020. Biochemical and clinical pregnancy were compared between groups.</p><p><strong>Results: </strong>No significant differences were observed in the endometrial lining, the total number of retrieved and metaphase-II oocytes, progesterone, and serum estradiol levels on human chorionic gonadotropin day, fertilization rate, and top-quality embryos between the groups. The duration of induction (10.8 <math><mo>±</mo></math> 1.7 vs. 10 <math><mo>±</mo></math> 2.1, p <math><mo><</mo></math> 0.001) and the total dosage of gonadotropins (2939.4 <math><mo>±</mo></math> 945.9 vs. 2441 <math><mo>±</mo></math> 1247.1, p <math><mo><</mo></math> 0.001) were significantly greater in group 2 than in group 1. No significant differences were observed between the 2 groups in terms of implantation rate, chemical pregnancy rate, and clinical pregnancy rate. A higher percentage of ovarian hyperstimulation syndrome was observed in group 2 (p = 0.005).</p><p><strong>Conclusion: </strong>Due to a lower percentage of ovarian hyperstimulation syndrome in group 1 and similar assisted reproductive technology outcomes in both groups, the long protocol was found to be superior to the short protocol.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"21 9","pages":"759-766"},"PeriodicalIF":1.3,"publicationDate":"2023-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10643683/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134648942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the effect of endometrial scratch by hysteroscopic scissors on frozen embryo transfer outcomes: A historical cohort study.","authors":"Sara Saedi, Amirhossein Tayebi, Maedeh Ghorbani Kahrizsangi, Fatemeh Jalalinezhad, Aryan Ayati, Alireza Hadizadeh, Bita Badehnoosh, Atousa Karimi","doi":"10.18502/ijrm.v21i9.14400","DOIUrl":"https://doi.org/10.18502/ijrm.v21i9.14400","url":null,"abstract":"<p><strong>Background: </strong>Endometrial scratch (ES) has been suggested to improve assisted reproductive techniques success rates by investigating implantation failure.</p><p><strong>Objective: </strong>In this study, we evaluated the effect of ES on the outcomes of frozen embryo transfer (FET) in women with at least 2 failed embryo transfer cycles.</p><p><strong>Materials and methods: </strong>In this historical cohort study, medical data of 236 infertile women who underwent in-vitro fertilization-FET at Ebne-sina Infertility Center, Tehran, Iran, from January 2015-December 2021 was extracted from their medical records. Based on having ES before FET, they were assigned to either the scratch (n = 118) or the no-scratch group (n = 118). We compared these groups regarding pregnancy rates and outcomes.</p><p><strong>Results: </strong>The demographic characteristics were similar in both groups regarding weight, body mass index, the number of previous embryo transfers, and the duration of infertility. However, the scratch group had a slightly higher mean age (32.31 vs. 29.96 yr, p <math><mo><</mo></math> 0.001). No statistically significant difference was observed between groups regarding pregnancy rate (p = 0.89). No significant association was observed between scratch, infertility duration, the number of previous FET attempts, and the likelihood of pregnancy in a logistic regression model. No major complications were observed.</p><p><strong>Conclusion: </strong>Hysteroscopic endometrial scratching with scissors probably has no effect on FET outcomes, including pregnancy or live birth rates.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"21 9","pages":"737-744"},"PeriodicalIF":1.3,"publicationDate":"2023-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10643685/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134648892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"The status of depression and anxiety in infertile Turkish couples\" [Iran J Reprod Med 2011; 9: 99-104].","authors":"Mert Kazandi,&nbsp;Ozlem Gunday,&nbsp;Timucin Kurtulus Mermer,&nbsp;Nuray Erturk,&nbsp;Erdinc Ozkınay","doi":"10.18502/ijrm.v21i8.14023","DOIUrl":"https://doi.org/10.18502/ijrm.v21i8.14023","url":null,"abstract":"<p><p>[This corrects the article on p. 99 in vol. 9 PMCPMC4216443.].</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"21 8","pages":"673"},"PeriodicalIF":1.3,"publicationDate":"2023-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10598472/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"54229082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The protective effect of hydroalcoholic extract of <i>Ephedra pachyclada</i> leaves on ovarian damage induced by cyclophosphamide in rat: An experimental study.","authors":"Hassanali Abedi,&nbsp;Mahnaz Nemati,&nbsp;Bahare Ebrahimi,&nbsp;Maryam Dehghani,&nbsp;Elmira Mikaeiliagah,&nbsp;Pegah Abdollahzadeh,&nbsp;Aref Ghanaatpishe,&nbsp;Nazanin Shafiee Jahromi,&nbsp;Hossein Kargar Jahromi","doi":"10.18502/ijrm.v21i8.14018","DOIUrl":"10.18502/ijrm.v21i8.14018","url":null,"abstract":"<p><strong>Background: </strong>Cyclophosphamide (CP) is an anticancer drug that acts as an alkylation agent after metabolism in the liver. CP has toxic effects on the body's cells, especially the reproductive system's function, and causes infertility. Moreover, medicinal plants have few side effects and are psychologically acceptable to patients.</p><p><strong>Objective: </strong>This study aimed to investigate the impact of <i>Ephedra pachyclada</i> hydroalcoholic extract (EPHE) on ovarian tissue and hypothalamic-pituitary-gonadal axis in rats treated with CP.</p><p><strong>Materials and methods: </strong>In this experimental study, 48 adult female Wistar rats (180-200 gr, 9-10 wk) were randomly assigned to 6 experimental groups (n = 8/each): (a) control; (b) sham; (c) CP; (d) CP+250 mg/kg EPHE; (e) CP+500 mg/kg EPHE; (f) CP+1000 mg/kg EPHE. On the 29<math><msup><mrow></mrow><mtext>th</mtext></msup></math> day of the experiment, serum was collected; serum concentration of the luteinizing hormone, follicle-stimulating hormone, estrogen, progesterone, and antioxidant activity were measured. The number of ovarian follicles were also counted.</p><p><strong>Results: </strong>In the CP groups, serum concentrations of follicle-stimulating hormone and luteinizing hormone significantly increased, and estrogen and progesterone significantly decreased (p <math><mo>≤</mo></math> 0.05). EPHE significantly compensated for the complications caused by CP and 1000 mg/kg had the greatest effect. Antioxidant reduction by CP was significantly enhanced by EPHE, especially at higher doses (p <math><mo>≤</mo></math> 0.05). The number of primordial, primary, secondary, and Graafian follicles showed a significant decrease in CP groups and EPHE groups showed a significant increase compared to the CP. EPHE showed that the concentration of 1000 mg/kg was more effective than other doses (p <math><mo>≤</mo></math> 0.05).</p><p><strong>Conclusion: </strong>In addition to proving the effect of EPHE on the hypothalamic-pituitary-gonadal axis, our investigation showed antioxidant properties, which can be an effective factor in CP-treated rats.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"21 8","pages":"629-638"},"PeriodicalIF":1.3,"publicationDate":"2023-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10598471/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"54229096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between anogenital distance as a noninvasive index in the diagnosis and prognosis of reproductive disorder: A systematic review.","authors":"Parisa Zamani,&nbsp;Zeinab Hemati,&nbsp;Roya Kelishadi,&nbsp;Sakineh Kolahdozan,&nbsp;Mostafa Dianatinasab,&nbsp;Mojtaba Keikha","doi":"10.18502/ijrm.v21i8.14016","DOIUrl":"10.18502/ijrm.v21i8.14016","url":null,"abstract":"<p><strong>Background: </strong>There are 2 measures of anogenital distance (AGD) in men and women. AGD has been used as an indicator of fetal androgen dysfunction and an adverse outcome in adulthood. Some studies have shown the association of AGD as a predictor in the diagnosis and prognosis of diseases and disorders.</p><p><strong>Objective: </strong>To systematically summarize the latest evidence for presenting AGD as a new approach for prognosis and early diagnosis of diseases.</p><p><strong>Materials and methods: </strong>A systematic review of the available literature was performed using Medline via PubMed, Scopus, and ISI Web of Knowledge up to July 2021, using search terms \"anogenital distance\" OR \"anogenital index\" OR \"ano genital distance\" OR \"ano genital index\". Language restrictions were not imposed.</p><p><strong>Results: </strong>After reviewing the retrieved articles, 47 unique studies were included in this systematic review. Different outcomes, including endometriosis, prostate cancer, polycystic ovary syndrome, pelvic organ prolapse, hypospadias, cryptorchidism, fertility and semen parameters, maternal and birth development, and ovarian and gynecological-related disorders, have been studied in the included evidence. A negative association was observed between AGD and endometriosis and hypospadias and a positive association between AGD and prostate cancer, polycystic ovary syndrome, male fetal gender, and fertility parameters.</p><p><strong>Conclusion: </strong>Using quantitative indicators such as AGD may be a useful clinical tool for the diagnosis of diseases. Although many studies have shown an association between AGD and diseases, some factors, including different measurement methods, different measurement tools, age, and different definitions of AGD, can be involved in the variation of AGD.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"21 8","pages":"599-618"},"PeriodicalIF":1.3,"publicationDate":"2023-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10598470/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"54229081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of congenital anomalies and related factors in live births in Zahedan, Southeast of Iran: A cross-sectional study.","authors":"Azam Asemi-Rad,&nbsp;Zahra Heidari,&nbsp;Hamidreza Mahmoudzadeh-Sagheb,&nbsp;Yousef Mehdipour,&nbsp;Bita Moudi,&nbsp;Nadia Sheibak,&nbsp;Saeid Ebrahimi","doi":"10.18502/ijrm.v21i8.14020","DOIUrl":"10.18502/ijrm.v21i8.14020","url":null,"abstract":"<p><strong>Background: </strong>The term congenital anomalies (CAs) refers to structural or functional abnormalities at the time of conception. Approximately 12 deaths related to congenital disabilities occur in every 10,000 babies born.</p><p><strong>Objective: </strong>This study aimed to evaluate the prevalence and associated factors of single and multiple CAs in live births in Zahedan, Southeast Iran.</p><p><strong>Materials and methods: </strong>This cross-sectional study was conducted on 59,087 live births in a referral hospital in Zahedan located in the southeast of Iran from 2009 to 2019. All live births were examined by pediatricians and the CAs and categorized based on the international classification of diseases.</p><p><strong>Results: </strong>Of 59,085 live births, at least 883 had a significant anomaly, and the prevalence rate of CAs was about 149 per 10,000. Anomalies of the nervous (24.1%) and cardiovascular systems (21.10%) were the most frequent, occurring in 213 and 187 of the live births, respectively. Spina bifida is the most common anomaly of the central nervous system. The most common anomalies in the cardiovascular system were unspecified heart malformations (17.1%), cardiovascular malformations (18.7%), and patent ductus arteriosus (11.7%). Significant correlations were found between the parent's consanguinity marriage, the mother's age, an existing anomaly in the family, and relatives in single and multiple CAs (p = 0.02, p = 0.02, p <math><mo><</mo></math> 0.001, p = 0.01, respectively).</p><p><strong>Conclusion: </strong>The prevalence of CAs was 149 per 10,000 live births. The highest prevalence of CAs was related to the central nervous system. Increasing the public's knowledge about fetal defects can reduce the prevalence of CAs.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"21 8","pages":"647-656"},"PeriodicalIF":1.3,"publicationDate":"2023-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10598469/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"54229095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Xeno-free generation of new Yazd human embryonic stem cell lines (Yazd4-7) as a prior stage toward good manufacturing practice of clinical-grade raw materials from discarded embryos: A lab resources report.","authors":"Fatemeh Hajizadeh-Tafti,&nbsp;Jalal Golzadeh,&nbsp;Fatemeh Akyash,&nbsp;Somayyeh-Sadat Tahajjodi,&nbsp;Ehsan Farashahi-Yazd,&nbsp;Hassan Heidarian-Meimandi,&nbsp;Behrouz Aflatoonian","doi":"10.18502/ijrm.v21i8.14017","DOIUrl":"10.18502/ijrm.v21i8.14017","url":null,"abstract":"<p><strong>Background: </strong>Xeno-free generation of human embryonic stem cells (hESCs) is important to prevent potential animal contaminations in culture for advanced cell-based therapeutic applications. Xeno-free production of hESCs is the first step for manufacturing clinical-grade hESC lines.</p><p><strong>Objective: </strong>To produce new hESC lines in xeno-free condition.</p><p><strong>Materials and methods: </strong>This lab resources report was conducted at Stem Cell Biology Research Center, Yazd, Iran from 2019-2022. 4 new hESC lines from 11 (10 fresh and 1 frozen) donated surplus discarded human embryos were established. In this study, we report the xeno-free derivation of new Yazd hESC lines (Yazd4-7), without using immunosurgery, by culturing intact zona-free blastocysts obtained from discarded embryos onto the YhFF#8 cells as a feeder layer in a microdrop culture system. The pluripotency gene expression profile of the cell lines was assessed by reverse transcription polymerase chain reaction and the expression of specific surface markers was detected using immunofluorescent staining. In vitro differentiation was induced using embryoid body formation and gene expression profile of 3 germ layers and germ cells. Reverse transcriptase polymerase chain reaction was investigated to prove their pluripotent capacity.</p><p><strong>Results: </strong>In sum, we have been able to generate 4 new hESC lines (Yazd4-7) from 11 discarded embryos in xeno-free culture conditions using a micro drop culture system and YhFF#8 as a human source feeder layer.</p><p><strong>Conclusion: </strong>The outcome of this work can be the foundation for the future allogeneic cell-based therapeutic application using clinical grade good manufacturing practice-derived hESC derivatives.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"21 8","pages":"619-628"},"PeriodicalIF":1.3,"publicationDate":"2023-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10598466/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"54229098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}