The possible regulatory role of miR-4463 and its target gene CYP19A1 on the ovarian response in the women with diminished ovarian reserve: A case-control study.

IF 1.6 Q3 OBSTETRICS & GYNECOLOGY
International Journal of Reproductive Biomedicine Pub Date : 2024-10-14 eCollection Date: 2024-08-01 DOI:10.18502/ijrm.v22i8.17237
Azam Yazdanian, Marzieh Lotfi, Fateme Montazeri, Saeideh Dashti, Mohammad Hasan Sheikhha
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引用次数: 0

Abstract

Background: Diminished ovarian reserve (DOR) is a condition that affects fertility by reducing the reproductive potential of the ovary. The altered expression profile of cumulus cells (CCs) can negatively affect the quality and quantity of oocytes in the ovaries. Recent studies suggest that circulating miRNAs play a significant role in the ovary function, and their serum expression changes can be valuable biomarkers for predicting ovarian function.

Objective: Investigating the expression levels of circulating miRNA-4463 and its target cytochrome P450 19A1 gene (CYP19A1) in DOR-CCs in order to find a molecular pathway involved in DOR.

Materials and methods: In this case-control study, a total of 20 DOR-women and 20 women with normal ovarian reservation aged between 20-34 yr referred to Yazd Reproductive Science Institute, Yazd, Iran were included in the study. Serum and CCs were collected, and real time-polymerase chain reaction was performed to investigate the expression level of miR-4463, and its target gene CYP19A1.

Results: Our results showed an inverse relationship between miR-4463 and CYP19A1 expression levels. Therefore, the increase in the expression of miR-4463 was significantly evident in DOR-women compared to the control group (p = 0.0019), while the expression of its target gene, CYP19A1, has significantly decreased in these women (p = 0.001).

Conclusion: The present study suggests that miR-4463 and CYP19A1 pathways could regulate ovary function. Therefore, examination of this miRNA could be a promising parameter for predicting ovarian reserve and their response to stimulation protocols.

miR-4463 及其靶基因 CYP19A1 对卵巢储备功能减退妇女卵巢反应的可能调控作用:一项病例对照研究
背景:卵巢储备功能减退(DOR)是一种通过降低卵巢生殖潜能来影响生育能力的疾病。积层细胞(CCs)表达谱的改变会对卵巢中卵母细胞的质量和数量产生负面影响。最新研究表明,循环 miRNA 在卵巢功能中发挥着重要作用,其血清表达变化可作为预测卵巢功能的重要生物标志物:调查DOR-CCs中循环miRNA-4463及其靶细胞色素P450 19A1基因(CYP19A1)的表达水平,以寻找参与DOR的分子途径:在这项病例对照研究中,共纳入了 20 名 DOR 妇女和 20 名在伊朗亚兹德生殖科学研究所(Yazd Reproductive Science Institute, Yazd, Iran)就诊的卵巢保留正常的 20-34 岁妇女。研究人员采集了血清和 CCs,并进行了实时聚合酶链反应,以调查 miR-4463 及其靶基因 CYP19A1 的表达水平:结果表明,miR-4463 与 CYP19A1 的表达水平呈反比关系。因此,与对照组相比,DOR 妇女中 miR-4463 的表达明显增加(p = 0.0019),而其靶基因 CYP19A1 的表达在这些妇女中明显下降(p = 0.001):本研究表明,miR-4463 和 CYP19A1 通路可调控卵巢功能。结论:本研究表明,miR-4463 和 CYP19A1 通路可调控卵巢功能,因此,对该 miRNA 的检测可能是预测卵巢储备功能及其对刺激方案反应的一个有前途的参数。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
2.40
自引率
7.70%
发文量
93
审稿时长
16 weeks
期刊介绍: The International Journal of Reproductive BioMedicine (IJRM), formerly published as "Iranian Journal of Reproductive Medicine (ISSN: 1680-6433)", is an international monthly scientific journal for who treat and investigate problems of infertility and human reproductive disorders. This journal accepts Original Papers, Review Articles, Short Communications, Case Reports, Photo Clinics, and Letters to the Editor in the fields of fertility and infertility, ethical and social issues of assisted reproductive technologies, cellular and molecular biology of reproduction including the development of gametes and early embryos, assisted reproductive technologies in model system and in a clinical environment, reproductive endocrinology, andrology, epidemiology, pathology, genetics, oncology, surgery, psychology, and physiology. Emerging topics including cloning and stem cells are encouraged.
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